Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032510 | XP_024636868.1 | 64.706 | 102 | 27 | 1 | 1 | 93 | 54 | 155 | 1.17e-31 | 131 |
| MsaG032510 | XP_024636868.1 | 64.831 | 236 | 39 | 1 | 132 | 323 | 89 | 324 | 1.48e-98 | 304 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032510 | tr|A0A396IHE6|A0A396IHE6_MEDTR | 64.706 | 102 | 27 | 1 | 1 | 93 | 54 | 155 | 5.60e-32 | 131 |
| MsaG032510 | tr|A0A396IHE6|A0A396IHE6_MEDTR | 64.831 | 236 | 39 | 1 | 132 | 323 | 89 | 324 | 7.05e-99 | 304 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG032510 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG004854 | MsaG032510 | 0.828758 | 1.347639e-54 | 3.764354e-52 |
| MsaG032510 | MsaG009144 | 0.806256 | 1.522182e-49 | 2.376725e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032510 | MtrunA17_Chr4g0067431 | 64.831 | 236 | 39 | 1 | 132 | 323 | 89 | 324 | 1.36e-102 | 304 |
| MsaG032510 | MtrunA17_Chr4g0067431 | 64.706 | 102 | 27 | 1 | 1 | 93 | 54 | 155 | 1.08e-35 | 131 |
| MsaG032510 | MtrunA17_Chr1g0163221 | 53.982 | 226 | 65 | 5 | 132 | 324 | 85 | 304 | 7.38e-62 | 201 |
| MsaG032510 | MtrunA17_Chr1g0163221 | 63.542 | 96 | 29 | 1 | 1 | 90 | 50 | 145 | 4.00e-30 | 117 |
| MsaG032510 | MtrunA17_Chr6g0467351 | 90.323 | 93 | 9 | 0 | 1 | 93 | 54 | 146 | 2.32e-56 | 182 |
| MsaG032510 | MtrunA17_Chr6g0467351 | 42.177 | 147 | 29 | 2 | 132 | 231 | 89 | 226 | 4.36e-24 | 98.2 |
| MsaG032510 | MtrunA17_Chr6g0467361 | 62.500 | 88 | 33 | 0 | 231 | 318 | 7 | 94 | 6.61e-34 | 120 |
| MsaG032510 | MtrunA17_Chr1g0163061 | 60.000 | 90 | 27 | 1 | 2 | 82 | 55 | 144 | 1.54e-25 | 103 |
| MsaG032510 | MtrunA17_Chr1g0163061 | 91.071 | 56 | 5 | 0 | 132 | 187 | 89 | 144 | 1.89e-18 | 84.0 |
| MsaG032510 | MtrunA17_Chr8g0390841 | 27.542 | 236 | 115 | 8 | 132 | 319 | 112 | 339 | 2.21e-13 | 70.9 |
| MsaG032510 | MtrunA17_Chr7g0233561 | 34.426 | 122 | 58 | 4 | 12 | 114 | 67 | 185 | 6.97e-12 | 65.5 |
| MsaG032510 | MtrunA17_Chr3g0131891 | 41.250 | 80 | 40 | 1 | 12 | 84 | 68 | 147 | 2.61e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTTCAATGTATGTCTTT+TGG | 0.144880 | 6:-54305002 | None:intergenic |
| GCTCACGATAATTTAAAATT+TGG | 0.151772 | 6:+54305974 | MsaT032510.1:CDS |
| CATCCTGTGCAGATGTTTCT+TGG | 0.182263 | 6:-54305840 | None:intergenic |
| AAATAAATTACCTTCAAAAT+AGG | 0.207151 | 6:-54306086 | None:intergenic |
| CTCTTTAATGTGATTGTATT+GGG | 0.236526 | 6:+54304502 | MsaT032510.1:CDS |
| CTTCATCATCACCTGGTAAA+AGG | 0.296364 | 6:+54300614 | MsaT032510.1:CDS |
| TCTCTTTAATGTGATTGTAT+TGG | 0.347950 | 6:+54304501 | MsaT032510.1:CDS |
| TTTGCGTGATTCACAACTTT+TGG | 0.378903 | 6:+54300474 | MsaT032510.1:CDS |
| AAACCAGTGATTATGATGTT+TGG | 0.387209 | 6:+54300413 | MsaT032510.1:CDS |
| AATAAATTACCTTCAAAATA+GGG | 0.393629 | 6:-54306085 | None:intergenic |
| TAACCTTCATGAAGGAAAAC+TGG | 0.398520 | 6:+54303863 | MsaT032510.1:CDS |
| CTCTTTGATGTGATTGTACT+CGG | 0.404696 | 6:+54300520 | MsaT032510.1:CDS |
| CTTCAGTAGTTTCTTTCAAA+GGG | 0.409737 | 6:-54304549 | None:intergenic |
| TGTTTCTTGGCAAATTCAAT+TGG | 0.419212 | 6:-54305827 | None:intergenic |
| AGGTAGTTGTGATGATCTTA+AGG | 0.427796 | 6:+54304906 | MsaT032510.1:CDS |
| AATAATCAATTTACACTAAG+TGG | 0.456060 | 6:+54305932 | MsaT032510.1:CDS |
| CGAGAGAGAAAATAGCAACT+TGG | 0.460403 | 6:-54306045 | None:intergenic |
| GAATTTGATCATCGGAGTAT+TGG | 0.472826 | 6:+54303810 | MsaT032510.1:CDS |
| TCTTCAGTAGTTTCTTTCAA+AGG | 0.474661 | 6:-54304550 | None:intergenic |
| TGACACAAATGAAAGTTACT+TGG | 0.482529 | 6:+54300386 | MsaT032510.1:CDS |
| GTTGGTGCAGGAAGGAAAGT+TGG | 0.505940 | 6:+54306467 | MsaT032510.1:intron |
| AATTTACACTAAGTGGTGGT+TGG | 0.508389 | 6:+54305939 | MsaT032510.1:CDS |
| TGGTTCTTTGAAAGTTAATG+AGG | 0.523148 | 6:+54300588 | MsaT032510.1:CDS |
| AAACCAAACATCATAATCAC+TGG | 0.538084 | 6:-54300416 | None:intergenic |
| GCACAGGATGGAAGGAAGAA+AGG | 0.547170 | 6:+54305853 | MsaT032510.1:CDS |
| TTTATATTAAACAAATGCAA+TGG | 0.550607 | 6:+54306010 | MsaT032510.1:CDS |
| AAACATCTGCACAGGATGGA+AGG | 0.555297 | 6:+54305845 | MsaT032510.1:CDS |
| CTTCAAAGTAACCTTCATGA+AGG | 0.561194 | 6:+54303855 | MsaT032510.1:CDS |
| CTTCTCAAGAATTTGATCAT+CGG | 0.561271 | 6:+54303802 | MsaT032510.1:CDS |
| GACACAAATGAAAGTTACTT+GGG | 0.564890 | 6:+54300387 | MsaT032510.1:CDS |
| TTGCCAAGAAACATCTGCAC+AGG | 0.566543 | 6:+54305837 | MsaT032510.1:CDS |
| ATCTCCATCACCATGTGTGA+AGG | 0.570345 | 6:+54300642 | MsaT032510.1:CDS |
| CAAACCTTCACACATGGTGA+TGG | 0.578924 | 6:-54300646 | None:intergenic |
| TTGTTGATAAAGACATAACA+TGG | 0.582622 | 6:+54305885 | MsaT032510.1:CDS |
| TGATTGTATTGGGTAAATGC+GGG | 0.584281 | 6:+54304512 | MsaT032510.1:CDS |
| GGTGCAGGAAGGAAAGTTGG+TGG | 0.585380 | 6:+54306470 | MsaT032510.1:intron |
| GTGATTGTATTGGGTAAATG+CGG | 0.585829 | 6:+54304511 | MsaT032510.1:CDS |
| AACTACTGAAGAATATGAAG+AGG | 0.597652 | 6:+54304561 | MsaT032510.1:CDS |
| TTCTTACAAACCTTCACACA+TGG | 0.605009 | 6:-54300652 | None:intergenic |
| AATCAATTTACACTAAGTGG+TGG | 0.617140 | 6:+54305935 | MsaT032510.1:CDS |
| ATACATTGAAAGAGATCACT+TGG | 0.619250 | 6:+54305011 | MsaT032510.1:CDS |
| CCTCCAAGAGAAAATTGTGA+TGG | 0.623137 | 6:+54300568 | MsaT032510.1:CDS |
| CAAGAAACATCTGCACAGGA+TGG | 0.624347 | 6:+54305841 | MsaT032510.1:CDS |
| GAGGATCCTTCATCATCACC+TGG | 0.635301 | 6:+54300607 | MsaT032510.1:CDS |
| GAGGATCCTTCATCATCGCG+AGG | 0.693909 | 6:+54304616 | MsaT032510.1:CDS |
| GTGATTGTACTCGGTAAATG+CGG | 0.696593 | 6:+54300529 | MsaT032510.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 54300385 | 54306499 | 54300385 | ID=MsaG032510 |
| Chr6 | mRNA | 54300385 | 54306499 | 54300385 | ID=MsaT032510.1;Parent=MsaG032510 |
| Chr6 | exon | 54300385 | 54300663 | 54300385 | ID=MsaT032510.1.exon1;Parent=MsaT032510.1 |
| Chr6 | CDS | 54300385 | 54300663 | 54300385 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Chr6 | exon | 54303771 | 54303884 | 54303771 | ID=MsaT032510.1.exon2;Parent=MsaT032510.1 |
| Chr6 | CDS | 54303771 | 54303884 | 54303771 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Chr6 | exon | 54304472 | 54304637 | 54304472 | ID=MsaT032510.1.exon3;Parent=MsaT032510.1 |
| Chr6 | CDS | 54304472 | 54304637 | 54304472 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Chr6 | exon | 54304902 | 54305032 | 54304902 | ID=MsaT032510.1.exon4;Parent=MsaT032510.1 |
| Chr6 | CDS | 54304902 | 54305032 | 54304902 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Chr6 | exon | 54305821 | 54306097 | 54305821 | ID=MsaT032510.1.exon5;Parent=MsaT032510.1 |
| Chr6 | CDS | 54305821 | 54306097 | 54305821 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Chr6 | exon | 54306477 | 54306499 | 54306477 | ID=MsaT032510.1.exon6;Parent=MsaT032510.1 |
| Chr6 | CDS | 54306477 | 54306499 | 54306477 | ID=cds.MsaT032510.1;Parent=MsaT032510.1 |
| Gene Sequence |
| Protein sequence |
