AlfalfaGEDB | MsaG032624

M. sativa ssp. caerulea / MsaG032624

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032624	KEH26198.1	89.503	181	19	0	26	206	156	336	1.96e-109	327

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032624	sp\|Q9T072\|BH025_ARATH	46.369	179	91	3	29	204	152	328	3.71e-41	145

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032624	tr\|A0A072UAR9\|A0A072UAR9_MEDTR	89.503	181	19	0	26	206	156	336	9.37e-110	327

TFs/TRs:

Gene ID	Type	Classification
MsaG032624	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002691	MsaG032624	0.811257	1.313982e-50	2.313832e-48
MsaG002692	MsaG032624	0.804954	2.847610e-49	4.312572e-47
MsaG006263	MsaG032624	0.800597	2.237383e-48	3.066214e-46
MsaG006521	MsaG032624	0.807976	6.608936e-50	1.074907e-47
MsaG012237	MsaG032624	0.800048	2.891322e-48	3.913466e-46
MsaG019176	MsaG032624	0.803001	7.222219e-49	1.045384e-46
MsaG024535	MsaG032624	0.800442	2.405384e-48	3.284928e-46
MsaG025587	MsaG032624	0.802565	8.876125e-49	1.272020e-46
MsaG032624	MsaG044874	0.804327	3.842938e-49	5.736056e-47
MsaG032624	MsaG047166	0.805464	2.229615e-49	3.416804e-47
MsaG032624	MsaG030428	0.813994	3.332409e-51	6.280913e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032624	MtrunA17_Chr6g0468491	90.449	178	17	0	29	206	137	314	1.12e-112	324
MsaG032624	MtrunA17_Chr6g0468491	69.048	42	13	0	1	42	1	42	1.17e-12	65.5
MsaG032624	MtrunA17_Chr2g0307131	77.401	177	40	0	29	205	179	355	1.82e-93	277
MsaG032624	MtrunA17_Chr2g0307141	75.843	178	43	0	29	206	183	360	2.18e-91	271
MsaG032624	MtrunA17_Chr4g0033391	71.667	180	48	1	25	204	143	319	7.71e-89	264
MsaG032624	MtrunA17_Chr4g0033291	74.176	182	44	1	25	206	151	329	2.21e-87	260
MsaG032624	MtrunA17_Chr7g0225901	79.618	157	32	0	25	181	84	240	1.46e-86	256
MsaG032624	MtrunA17_Chr4g0033281	72.928	181	45	1	25	205	149	325	2.66e-79	240
MsaG032624	MtrunA17_Chr4g0054581	66.667	183	55	2	29	206	198	379	8.33e-75	230
MsaG032624	MtrunA17_Chr4g0054621	58.791	182	68	3	25	206	168	342	4.76e-66	206
MsaG032624	MtrunA17_Chr6g0468461	71.975	157	28	3	26	181	170	311	5.31e-64	201
MsaG032624	MtrunA17_Chr6g0468461	66.667	42	14	0	1	42	23	64	9.50e-12	63.2
MsaG032624	MtrunA17_Chr5g0400621	56.593	182	79	0	24	205	152	333	3.18e-61	194
MsaG032624	MtrunA17_Chr5g0400641	54.375	160	65	3	32	187	1	156	6.98e-48	154
MsaG032624	MtrunA17_Chr4g0002801	42.935	184	99	1	25	202	148	331	4.62e-46	155
MsaG032624	MtrunA17_Chr4g0049331	46.154	182	96	2	24	204	130	310	6.18e-44	149
MsaG032624	MtrunA17_Chr2g0280161	45.405	185	94	4	24	204	172	353	6.78e-44	149
MsaG032624	MtrunA17_Chr5g0400661	43.850	187	90	4	29	201	149	334	4.30e-43	147
MsaG032624	MtrunA17_Chr4g0054551	44.379	169	90	2	29	196	155	320	1.04e-41	143
MsaG032624	MtrunA17_Chr2g0333621	42.162	185	100	3	25	205	134	315	1.59e-41	142
MsaG032624	MtrunA17_Chr4g0054561	45.562	169	88	3	29	196	155	320	2.21e-41	142
MsaG032624	MtrunA17_Chr2g0333641	44.509	173	92	3	25	196	133	302	4.87e-41	141
MsaG032624	MtrunA17_Chr4g0002781	40.884	181	104	1	25	202	170	350	5.12e-40	139
MsaG032624	MtrunA17_Chr4g0054571	45.213	188	90	3	26	203	147	331	1.69e-39	137
MsaG032624	MtrunA17_Chr5g0400671	43.275	171	94	2	29	199	154	321	2.85e-38	134
MsaG032624	MtrunA17_Chr8g0336681	41.011	178	94	4	29	197	156	331	1.04e-34	125
MsaG032624	MtrunA17_Chr2g0333611	38.418	177	101	3	30	203	46	217	3.67e-34	121
MsaG032624	MtrunA17_Chr7g0249811	43.195	169	87	3	29	197	165	324	4.12e-34	124
MsaG032624	MtrunA17_Chr2g0333541	42.473	186	89	3	25	200	90	267	1.21e-33	121
MsaG032624	MtrunA17_Chr4g0002831	36.216	185	91	7	22	198	36	201	1.45e-25	98.6
MsaG032624	MtrunA17_Chr4g0002851	36.129	155	67	3	52	199	65	194	1.46e-23	93.2
MsaG032624	MtrunA17_Chr7g0233441	34.839	155	77	4	52	199	57	194	3.38e-22	89.4
MsaG032624	MtrunA17_Chr1g0185621	29.508	183	110	4	29	196	475	653	2.19e-17	79.7
MsaG032624	MtrunA17_Chr8g0366751	32.877	146	83	2	28	160	461	604	1.00e-16	77.8
MsaG032624	MtrunA17_Chr8g0346161	30.986	142	79	5	28	158	290	423	6.13e-16	75.5
MsaG032624	MtrunA17_Chr7g0275821	30.968	155	99	4	28	182	251	397	1.68e-15	73.9
MsaG032624	MtrunA17_Chr5g0411341	28.662	157	94	2	28	166	492	648	1.22e-14	72.0
MsaG032624	MtrunA17_Chr8g0346151	33.051	118	69	3	28	138	278	392	3.83e-13	67.4
MsaG032624	MtrunA17_Chr8g0386921	46.154	65	35	0	28	92	405	469	5.59e-13	67.0
MsaG032624	MtrunA17_Chr7g0260841	49.123	57	29	0	28	84	354	410	1.93e-12	65.5
MsaG032624	MtrunA17_Chr8g0365711	24.224	161	101	3	31	178	246	398	3.23e-12	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032624	AT4G37850.2	46.369	179	91	3	29	204	152	328	3.77e-42	145
MsaG032624	AT4G37850.1	46.369	179	91	3	29	204	152	328	3.77e-42	145
MsaG032624	AT2G22760.1	44.944	178	88	5	29	199	119	293	4.04e-40	139
MsaG032624	AT2G22750.2	43.094	181	95	3	29	203	126	304	6.08e-36	128
MsaG032624	AT2G22750.3	43.094	181	95	3	29	203	154	332	6.98e-36	129
MsaG032624	AT2G22750.5	43.094	181	95	3	29	203	150	328	7.96e-36	128
MsaG032624	AT2G22750.1	43.094	181	94	4	29	203	126	303	1.41e-34	124
MsaG032624	AT2G22750.4	43.094	181	94	4	29	203	154	331	1.55e-34	125
MsaG032624	AT2G22770.1	39.247	186	98	2	29	199	132	317	4.11e-34	124
MsaG032624	AT2G22760.2	44.025	159	80	4	29	181	119	274	2.15e-33	121
MsaG032624	AT5G46760.1	30.939	181	100	7	28	185	414	592	9.47e-18	81.3
MsaG032624	AT4G17880.1	35.811	148	78	4	28	163	415	557	4.04e-17	79.3
MsaG032624	AT1G32640.1	32.886	149	88	3	28	166	451	597	1.14e-16	78.2
MsaG032624	AT1G63650.3	30.714	140	79	3	29	150	405	544	2.96e-14	71.2
MsaG032624	AT1G63650.1	30.714	140	79	3	29	150	405	544	2.96e-14	71.2
MsaG032624	AT1G63650.2	30.714	140	79	3	29	150	405	544	2.96e-14	71.2
MsaG032624	AT1G01260.1	37.500	96	57	1	28	123	432	524	4.79e-14	70.5
MsaG032624	AT1G01260.2	37.500	96	57	1	28	123	432	524	4.79e-14	70.5
MsaG032624	AT1G01260.3	37.500	96	57	1	28	123	432	524	4.79e-14	70.5
MsaG032624	AT2G46510.1	46.479	71	35	1	28	95	394	464	1.24e-13	69.3
MsaG032624	AT4G09820.1	33.065	124	63	1	27	150	361	464	1.55e-13	68.9
MsaG032624	AT5G41315.1	43.478	69	39	0	29	97	441	509	1.78e-13	68.9
MsaG032624	AT5G41315.2	43.478	69	39	0	29	97	449	517	1.80e-13	68.9
MsaG032624	AT5G41315.3	43.478	69	39	0	29	97	446	514	1.84e-13	68.9
MsaG032624	AT4G16430.1	45.763	59	32	0	28	86	319	377	2.77e-12	65.1
MsaG032624	AT2G16910.1	26.452	155	92	4	30	162	315	469	7.19e-11	61.2

Find 33 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGACTTCTAGTTAAAATTT+TGG	0.191143	6:+57649025	MsaT032624.1:CDS
CAAGTGGAAGCAAGAATCTT+AGG	0.299386	6:+57648963	MsaT032624.1:CDS
CTAGAAGTCCTTTGTGCTCT+TGG	0.306653	6:-57649013	None:intergenic
TTACTTAATGTACCTTCTTA+AGG	0.323115	6:-57648630	None:intergenic
CATCTGTATCAAGGAAATTC+AGG	0.330296	6:-57648175	None:intergenic
GTCCTTTGTGCTCTTGGCAT+TGG	0.348569	6:-57649007	None:intergenic
ATTCTTGCTTCCACTTGAAA+TGG	0.371460	6:-57648957	None:intergenic
ATCGTTGCAGAGGCCTAGTT+TGG	0.384101	6:-57648886	None:intergenic
AATAATCGTGTCTTACCCTT+TGG	0.384577	6:+57649080	MsaT032624.1:CDS
TAGGAAAATATATGTTGATT+CGG	0.438258	6:+57648982	MsaT032624.1:CDS
TAATGTACCTTCTTAAGGTT+AGG	0.447983	6:-57648625	None:intergenic
AAATTCAGGTTGCTTTCCTC+AGG	0.450905	6:-57648161	None:intergenic
CCGACAGTCTTGACCAAACT+AGG	0.471259	6:+57648873	MsaT032624.1:CDS
AGTTTCTACCTGAGCAATGA+TGG	0.495762	6:-57649123	None:intergenic
TCTTGTTCCTAACCTTAAGA+AGG	0.522439	6:+57648618	MsaT032624.1:CDS
AGACATTACCATCATTGCTC+AGG	0.528978	6:+57649115	MsaT032624.1:CDS
TCAGAACTTCATTGCTCTTG+CGG	0.540285	6:+57648594	MsaT032624.1:CDS
ACTCTACCACATCTGTATCA+AGG	0.544071	6:-57648184	None:intergenic
TGAATACAATTTATTTCCTG+AGG	0.546737	6:+57648145	MsaT032624.1:CDS
GAGCAATGATGGTAATGTCT+AGG	0.547782	6:-57649112	None:intergenic
AGGTTACAACATGAGCATAA+AGG	0.548654	6:+57649421	MsaT032624.1:CDS
TCATGGTCAAGATCATATCA+TGG	0.581035	6:+57648543	MsaT032624.1:intron
TGTTGATTTACCATTTCAAG+TGG	0.584256	6:+57648947	MsaT032624.1:CDS
GAGTTTCTCTCACATGACAT+AGG	0.592458	6:+57648203	MsaT032624.1:CDS
ACTTCAATGCTGCCATGCGT+AGG	0.601033	6:-57649457	None:intergenic
GTCTAGGATAGAATTTCCAA+AGG	0.606376	6:-57649096	None:intergenic
TCTAGGATAGAATTTCCAAA+GGG	0.607454	6:-57649095	None:intergenic
CCTAGTTTGGTCAAGACTGT+CGG	0.617301	6:-57648873	None:intergenic
GAATTTCCTTGATACAGATG+TGG	0.638336	6:+57648178	MsaT032624.1:CDS
AGGAGTTATCATCGTTGCAG+AGG	0.639176	6:-57648896	None:intergenic
ACTTGTGAAGAACCTACGCA+TGG	0.684270	6:+57649445	MsaT032624.1:CDS
ATCCAATGCCAAGAGCACAA+AGG	0.699971	6:+57649005	MsaT032624.1:CDS
AATGCTCTCATGAGATGACG+AGG	0.706550	6:-57648916	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	57648140	57649493	57648140	ID=MsaG032624
Chr6	mRNA	57648140	57649493	57648140	ID=MsaT032624.1;Parent=MsaG032624
Chr6	exon	57648140	57648224	57648140	ID=MsaT032624.1.exon1;Parent=MsaT032624.1
Chr6	CDS	57648140	57648224	57648140	ID=cds.MsaT032624.1;Parent=MsaT032624.1
Chr6	exon	57648554	57648639	57648554	ID=MsaT032624.1.exon2;Parent=MsaT032624.1
Chr6	CDS	57648554	57648639	57648554	ID=cds.MsaT032624.1;Parent=MsaT032624.1
Chr6	exon	57648768	57649136	57648768	ID=MsaT032624.1.exon3;Parent=MsaT032624.1
Chr6	CDS	57648768	57649136	57648768	ID=cds.MsaT032624.1;Parent=MsaT032624.1
Chr6	exon	57649413	57649493	57649413	ID=MsaT032624.1.exon4;Parent=MsaT032624.1
Chr6	CDS	57649413	57649493	57649413	ID=cds.MsaT032624.1;Parent=MsaT032624.1

Gene Sequence

>MsaT032624.1

ATGGATGAATACAATTTATTTCCTGAGGAAAGCAACCTGAATTTCCTTGATACAGATGTGGTAGAGTTTCTCTCACATGACATAGATCATATCATGGCTGAGAGAAAGAGAAGAGAGAAACTCACTCAGAACTTCATTGCTCTTGCGGCTCTTGTTCCTAACCTTAAGAAGGTGGACAAGTATTCTGTATTAGTTGACACTATCAAATACTTGAAAGAACTTAAAAAGCGTTTGAAATTTTTGGAAGAACAAAATGAGAAAACAAAAATAGAGTCACCGACAGTCTTGACCAAACTAGGCCTCTGCAACGATGATAACTCCTCGTCATCTCATGAGAGCATTGATTGTGTTGTTGATTTACCATTTCAAGTGGAAGCAAGAATCTTAGGAAAATATATGTTGATTCGGATCCAATGCCAAGAGCACAAAGGACTTCTAGTTAAAATTTTGGTTGAGGTTCAAAGGTTTCAACTATTTGTTGTCAATAATCGTGTCTTACCCTTTGGAAATTCTATCCTAGACATTACCATCATTGCTCAGTTGGGTGAAGGTTACAACATGAGCATAAAGGAACTTGTGAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCATAA

Protein sequence

>MsaT032624.1

MDEYNLFPEESNLNFLDTDVVEFLSHDIDHIMAERKRREKLTQNFIALAALVPNLKKVDKYSVLVDTIKYLKELKKRLKFLEEQNEKTKIESPTVLTKLGLCNDDNSSSSHESIDCVVDLPFQVEARILGKYMLIRIQCQEHKGLLVKILVEVQRFQLFVVNNRVLPFGNSILDITIIAQLGEGYNMSIKELVKNLRMAALKFMSS*