AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032789 MtrunA17_Chr6g0481091 35.032 157 80 7 4 146 6 154 6.28e-18 79.7
MsaG032789 MtrunA17_Chr4g0056541 33.758 157 81 6 4 146 7 154 1.62e-17 80.5
MsaG032789 MtrunA17_Chr5g0398971 33.333 159 81 8 4 146 7 156 2.38e-17 79.7
MsaG032789 MtrunA17_Chr5g0405211 33.117 154 86 5 4 146 8 155 4.89e-17 79.0
MsaG032789 MtrunA17_Chr1g0200561 32.484 157 89 5 4 149 19 169 5.52e-17 79.0
MsaG032789 MtrunA17_Chr4g0050701 30.380 158 86 6 4 147 11 158 1.12e-16 75.5
MsaG032789 MtrunA17_Chr1g0183741 34.641 153 82 7 4 146 10 154 2.07e-16 76.3
MsaG032789 MtrunA17_Chr7g0263931 33.750 160 80 7 4 145 11 162 5.57e-16 75.9
MsaG032789 MtrunA17_Chr2g0318101 33.121 157 84 6 4 154 15 156 7.00e-16 75.9
MsaG032789 MtrunA17_Chr8g0372121 33.766 154 85 5 4 146 6 153 9.93e-16 74.7
MsaG032789 MtrunA17_Chr4g0047841 35.556 135 74 5 4 132 15 142 1.86e-15 74.3
MsaG032789 MtrunA17_Chr5g0417791 30.625 160 85 6 4 146 3 153 2.17e-15 73.2
MsaG032789 MtrunA17_Chr2g0326361 31.447 159 85 5 4 145 14 165 4.50e-15 73.2
MsaG032789 MtrunA17_Chr4g0017921 31.351 185 101 10 4 175 8 179 5.27e-15 73.2
MsaG032789 MtrunA17_Chr8g0345671 34.416 154 80 8 4 145 11 155 5.97e-15 73.2
MsaG032789 MtrunA17_Chr3g0128151 34.375 160 77 10 4 146 22 170 1.43e-14 72.4
MsaG032789 MtrunA17_Chr1g0147421 32.692 156 88 6 2 146 25 174 1.70e-14 71.6
MsaG032789 MtrunA17_Chr3g0128111 33.544 158 75 10 7 146 45 190 5.08e-14 69.3
MsaG032789 MtrunA17_Chr8g0361731 29.940 167 88 7 2 146 12 171 5.53e-14 70.5
MsaG032789 MtrunA17_Chr8g0345691 31.410 156 88 6 1 145 9 156 7.37e-14 70.1
MsaG032789 MtrunA17_Chr1g0197351 34.356 163 79 7 4 146 42 196 1.10e-13 69.7
MsaG032789 MtrunA17_Chr3g0144271 31.757 148 89 5 4 145 23 164 1.15e-13 69.7
MsaG032789 MtrunA17_Chr4g0076731 35.294 153 78 8 6 146 56 199 1.22e-13 69.7
MsaG032789 MtrunA17_Chr6g0453131 30.723 166 83 8 4 146 15 171 2.50e-13 68.6
MsaG032789 MtrunA17_Chr5g0428991 34.161 161 76 10 4 146 25 173 4.43e-13 68.2
MsaG032789 MtrunA17_Chr7g0214071 31.613 155 83 7 4 146 15 158 9.44e-13 65.9
MsaG032789 MtrunA17_Chr8g0346001 27.381 168 88 7 4 146 15 173 1.03e-12 67.0
MsaG032789 MtrunA17_Chr6g0484441 32.051 156 86 7 2 146 3 149 1.18e-12 66.2
MsaG032789 MtrunA17_Chr7g0252971 28.571 168 87 8 4 146 8 167 7.53e-12 63.9
MsaG032789 MtrunA17_Chr2g0316971 30.380 158 88 5 4 146 23 173 1.04e-11 63.9
MsaG032789 MtrunA17_Chr8g0345711 32.000 150 79 8 2 137 10 150 1.15e-11 61.6
MsaG032789 MtrunA17_Chr4g0060931 31.646 158 84 8 4 146 17 165 2.43e-11 62.8
MsaG032789 MtrunA17_Chr3g0130321 29.518 166 93 5 4 152 7 165 4.00e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032789 AT4G36160.1 33.544 158 88 5 4 150 10 161 7.63e-19 84.7
MsaG032789 AT4G36160.3 33.544 158 88 5 4 150 10 161 7.63e-19 84.7
MsaG032789 AT4G36160.2 33.544 158 88 5 4 150 14 165 9.18e-19 84.3
MsaG032789 AT1G71930.2 33.121 157 88 5 4 149 9 159 7.55e-18 81.3
MsaG032789 AT1G71930.1 33.121 157 88 5 4 149 9 159 7.55e-18 81.3
MsaG032789 AT2G24430.2 35.669 157 82 6 2 146 14 163 8.68e-18 81.3
MsaG032789 AT2G24430.1 35.669 157 82 6 2 146 14 163 8.68e-18 81.3
MsaG032789 AT2G18060.3 33.117 154 86 5 4 146 9 156 3.05e-17 80.1
MsaG032789 AT2G18060.1 33.117 154 86 5 4 146 9 156 3.05e-17 80.1
MsaG032789 AT4G28530.1 33.133 166 85 5 4 147 10 171 4.37e-16 76.6
MsaG032789 AT1G69490.1 32.500 160 82 7 4 146 9 159 4.87e-16 75.5
MsaG032789 AT1G26870.1 33.974 156 86 6 2 149 14 160 9.93e-16 75.9
MsaG032789 AT3G10490.1 33.544 158 82 7 4 146 27 176 3.23e-15 72.8
MsaG032789 AT4G10350.1 33.962 159 80 9 4 146 9 158 3.32e-15 73.9
MsaG032789 AT2G27300.1 29.167 144 92 4 6 145 16 153 3.87e-15 73.9
MsaG032789 AT4G17980.1 33.333 159 82 7 4 145 6 157 7.04e-15 72.4
MsaG032789 AT4G17980.2 33.125 160 83 7 3 145 5 157 1.06e-14 72.0
MsaG032789 AT1G01720.1 32.298 161 82 6 4 147 7 157 1.14e-14 72.0
MsaG032789 AT5G64530.2 33.588 131 74 4 4 134 3 120 1.36e-14 69.3
MsaG032789 AT5G63790.1 33.333 162 80 7 4 147 50 201 1.57e-14 72.0
MsaG032789 AT1G54330.1 32.026 153 87 6 4 146 6 151 1.59e-14 71.6
MsaG032789 AT5G64530.1 33.588 131 74 4 4 134 3 120 1.59e-14 70.1
MsaG032789 AT5G63790.2 33.333 162 80 7 4 147 60 211 1.90e-14 71.6
MsaG032789 AT1G61110.1 33.577 137 77 5 4 134 16 144 1.04e-13 69.7
MsaG032789 AT5G39610.1 32.278 158 83 7 4 146 20 168 1.23e-13 68.9
MsaG032789 AT3G10480.2 33.121 157 83 7 4 146 27 175 1.42e-13 69.7
MsaG032789 AT4G35580.3 31.902 163 89 8 1 149 6 160 1.65e-13 69.7
MsaG032789 AT4G35580.2 31.902 163 89 8 1 149 6 160 1.78e-13 69.7
MsaG032789 AT4G35580.1 31.902 163 89 8 1 149 6 160 1.88e-13 69.7
MsaG032789 AT5G04410.1 31.646 158 84 6 4 146 9 157 2.44e-13 69.3
MsaG032789 AT1G32770.1 28.729 181 85 8 4 149 16 187 3.24e-13 68.6
MsaG032789 AT5G39820.1 35.338 133 73 6 6 132 22 147 5.05e-13 67.8
MsaG032789 AT3G10500.1 32.278 158 83 7 4 146 9 157 5.94e-13 68.2
MsaG032789 AT3G18400.1 31.646 158 87 8 2 147 3 151 7.84e-13 67.0
MsaG032789 AT5G22290.1 26.178 191 116 7 6 179 23 205 8.77e-13 67.0
MsaG032789 AT2G46770.1 27.907 172 86 7 4 146 16 178 9.07e-13 67.0
MsaG032789 AT5G13180.1 30.464 151 91 5 4 146 14 158 1.12e-12 65.9
MsaG032789 AT3G29035.1 31.013 158 85 6 4 146 24 172 1.35e-12 66.2
MsaG032789 AT3G15510.1 32.576 132 76 5 10 135 23 147 1.74e-12 66.2
MsaG032789 AT4G27410.3 29.240 171 86 7 4 146 14 177 2.91e-12 65.5
MsaG032789 AT5G61430.1 32.278 158 83 7 4 146 16 164 3.19e-12 65.5
MsaG032789 AT1G32870.1 32.331 133 79 5 4 132 10 135 4.43e-12 65.5
MsaG032789 AT1G32870.2 32.331 133 79 5 4 132 10 135 4.62e-12 65.5
MsaG032789 AT2G17040.1 30.323 155 82 7 6 146 8 150 5.18e-12 64.3
MsaG032789 AT1G32870.3 32.331 133 79 5 4 132 46 171 6.20e-12 65.1
MsaG032789 AT3G04060.1 33.333 159 81 9 4 146 20 169 8.28e-12 64.3
MsaG032789 AT5G24590.2 30.247 162 82 8 4 146 13 162 1.12e-11 64.3
MsaG032789 AT5G53950.1 29.375 160 91 5 2 146 15 167 3.01e-11 62.8
MsaG032789 AT5G04395.1 31.373 153 82 7 4 138 28 175 3.02e-11 60.8
MsaG032789 AT5G46590.2 32.374 139 80 6 4 135 6 137 3.63e-11 62.0
MsaG032789 AT1G34190.1 30.519 154 87 6 6 146 18 164 3.65e-11 62.8
MsaG032789 AT1G02250.1 26.160 237 145 10 5 229 4 222 4.25e-11 62.4
MsaG032789 AT3G04420.2 26.490 151 96 6 5 146 4 148 4.47e-11 62.0
MsaG032789 AT3G04420.1 26.490 151 96 6 5 146 4 148 5.40e-11 62.0

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTAATGCTTTCAATTA+TGG 0.184656 6:+63113184 MsaT032789.1:CDS
CAATGGTTCTGACTTGATTA+AGG 0.258416 6:+63111037 MsaT032789.1:CDS
GGCAATGTAAGATTGGATTT+TGG 0.279853 6:+63111171 MsaT032789.1:CDS
CCTTGGGTGACGGAAGAAAA+AGG 0.350861 6:-63111108 None:intergenic
ATTGAAAGCATTAGTTGCTC+TGG 0.380713 6:-63113179 None:intergenic
ATGGATCTGGATGTTGAGTT+TGG 0.395501 6:+63111497 MsaT032789.1:CDS
CCTCATCTGCTCATCAACTT+TGG 0.414860 6:-63111150 None:intergenic
AACGGTTACTCGTAAACCTA+TGG 0.427747 6:+63111570 MsaT032789.1:CDS
GAAGAGGTTCAAAATTTCAA+TGG 0.434760 6:+63111020 MsaT032789.1:CDS
GGAGTAAGACGCAATTCAAA+TGG 0.441267 6:-63111083 None:intergenic
GCTGTTCGAGACATTATTGT+TGG 0.457958 6:+63111206 MsaT032789.1:CDS
GCAATTCAAATGGGTCATAC+TGG 0.459319 6:-63111073 None:intergenic
CAAATGGGTCATACTGGTAC+AGG 0.462094 6:-63111067 None:intergenic
CAGATGAGGCAATGTAAGAT+TGG 0.462721 6:+63111164 MsaT032789.1:CDS
TTGAAAGCATTAGTTGCTCT+GGG 0.473709 6:-63113178 None:intergenic
GAATTGCTTCCACCTGGTGT+TGG 0.483908 6:+63110942 MsaT032789.1:CDS
AACACACGACAAAGAACAAA+AGG 0.484780 6:-63111458 None:intergenic
TCAATTAATTTACACAAGAT+CGG 0.501330 6:-63113228 None:intergenic
TAACCGTTCGACATTGTTAG+CGG 0.507502 6:-63111555 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 6:-63111125 None:intergenic
AGAATGGCATCAAAACTAGT+TGG 0.512078 6:+63111291 MsaT032789.1:CDS
ACGGTTACTCGTAAACCTAT+GGG 0.512389 6:+63111571 MsaT032789.1:CDS
CTGCCCACCAGAGATGGATC+TGG 0.513629 6:+63111484 MsaT032789.1:CDS
TCAACATCCAGATCCATCTC+TGG 0.528153 6:-63111491 None:intergenic
CACTCTTGCTAACAATCCTC+AGG 0.528444 6:+63111328 MsaT032789.1:CDS
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 6:-63111118 None:intergenic
GAGTAAGACGCAATTCAAAT+GGG 0.549774 6:-63111082 None:intergenic
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 6:-63111124 None:intergenic
CCAAAGTTGATGAGCAGATG+AGG 0.560720 6:+63111150 MsaT032789.1:CDS
ACATCCAGATCCATCTCTGG+TGG 0.568257 6:-63111488 None:intergenic
GAAGGGATGAAGCCAACACC+AGG 0.588550 6:-63110954 None:intergenic
TTTGGAGCAGATGTTGATGA+AGG 0.590115 6:+63111515 MsaT032789.1:CDS
ATAGGAAATTCCGCTCAGAA+TGG 0.595921 6:+63111275 MsaT032789.1:CDS
GTTTGACTGCCCACCAGAGA+TGG 0.602762 6:+63111478 MsaT032789.1:CDS
TGTTGGAACTGATAACGTGT+TGG 0.614058 6:+63111223 MsaT032789.1:CDS
TAACAAGGAGTTCATCAGAA+GGG 0.616296 6:-63110971 None:intergenic
CGGTTACTCGTAAACCTATG+GGG 0.619101 6:+63111572 MsaT032789.1:CDS
GCAATGGAATTGCTTCCACC+TGG 0.624048 6:+63110936 None:intergenic
TGGAACTGATAACGTGTTGG+TGG 0.634013 6:+63111226 MsaT032789.1:CDS
TGACGATGTTGTCTTGCACA+TGG 0.636821 6:+63113145 MsaT032789.1:CDS
TGGCAAGGTAGTAGATAACA+AGG 0.641793 6:-63110986 None:intergenic
ATAACAAGGAGTTCATCAGA+AGG 0.650392 6:-63110972 None:intergenic
CATCCAGATCCATCTCTGGT+GGG 0.656418 6:-63111487 None:intergenic
GGTTACTCGTAAACCTATGG+GGG 0.660106 6:+63111573 MsaT032789.1:CDS
GATGAAGGTGATGCTCGAGA+TGG 0.661006 6:+63111530 MsaT032789.1:CDS
GGGATGAAGCCAACACCAGG+TGG 0.665656 6:-63110951 None:intergenic
GGAACTGATAACGTGTTGGT+GGG 0.675682 6:+63111227 MsaT032789.1:CDS
AGTGCTGCTGTTGAGCAACG+CGG 0.701822 6:+63111616 MsaT032789.1:CDS
TGAAAGCATTAGTTGCTCTG+GGG 0.714933 6:-63113177 None:intergenic
GCTCCGCTAACAATGTCGAA+CGG 0.715597 6:+63111552 MsaT032789.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 63110939 63113241 63110939 ID=MsaG032789
Chr6 mRNA 63110939 63113241 63110939 ID=MsaT032789.1;Parent=MsaG032789
Chr6 exon 63110939 63111349 63110939 ID=MsaT032789.1.exon1;Parent=MsaT032789.1
Chr6 CDS 63110939 63111349 63110939 ID=cds.MsaT032789.1;Parent=MsaT032789.1
Chr6 exon 63111455 63111637 63111455 ID=MsaT032789.1.exon2;Parent=MsaT032789.1
Chr6 CDS 63111455 63111637 63111455 ID=cds.MsaT032789.1;Parent=MsaT032789.1
Chr6 exon 63113125 63113241 63113125 ID=MsaT032789.1.exon3;Parent=MsaT032789.1
Chr6 CDS 63113125 63113241 63113125 ID=cds.MsaT032789.1;Parent=MsaT032789.1
Gene Sequence

>MsaT032789.1

ATGGAATTGCTTCCACCTGGTGTTGGCTTCATCCCTTCTGATGAACTCCTTGTTATCTACTACCTTGCCAACAAAAATAATGAAGAGGTTCAAAATTTCAATGGTTCTGACTTGATTAAGGAGCTAAACCTGTACCAGTATGACCCATTTGAATTGCGTCTTACTCCGTCCTTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGATGAGGCAATGTAAGATTGGATTTTGGATGCAGAAGGGGGCTGTTCGAGACATTATTGTTGGAACTGATAACGTGTTGGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCGCTCAGAATGGCATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGCTAACAATCCTCAGGCTCCTTTTGTTCTTTGTCGTGTGTTTGACTGCCCACCAGAGATGGATCTGGATGTTGAGTTTGGAGCAGATGTTGATGAAGGTGATGCTCGAGATGGCTCCGCTAACAATGTCGAACGGTTACTCGTAAACCTATGGGGGCTAGGGATGGGGCTGGTACTAGTGCTGCTGTTGAGCAACGCGGCGAGTTATGCTGCATTTCTTGACGATGTTGTCTTGCACATGGAAGCTATGACCCCAGAGCAACTAATGCTTTCAATTATGGATGACGATTTTCTTGAAATAGCCGATCTTGTGTAA
Protein sequence

>MsaT032789.1

MELLPPGVGFIPSDELLVIYYLANKNNEEVQNFNGSDLIKELNLYQYDPFELRLTPSFFFRHPREFYCFTAKVDEQMRQCKIGFWMQKGAVRDIIVGTDNVLVGKKSLFMFYIGNSAQNGIKTSWTMYEYTLANNPQAPFVLCRVFDCPPEMDLDVEFGADVDEGDARDGSANNVERLLVNLWGLGMGLVLVLLLSNAASYAAFLDDVVLHMEAMTPEQLMLSIMDDDFLEIADLV*