AlfalfaGEDB | MsaG032791

M. sativa ssp. caerulea / MsaG032791

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009889 GO:0010035 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032791	QSD99880.1	64.220	109	38	1	1	108	1	109	4.00e-41	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032791	sp\|Q6H8A9\|NAC23_ORYSJ	35.714	140	80	5	5	137	13	149	2.04e-16	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032791	tr\|A0A896WD20\|A0A896WD20_MELAB	64.220	109	38	1	1	108	1	109	1.91e-41	147

TFs/TRs:

Gene ID	Type	Classification
MsaG032791	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG011808	MsaG032791	0.834176	6.337472e-56	2.071077e-53
MsaG045786	MsaG032791	0.806554	1.318315e-49	2.072897e-47
MsaG026746	MsaG032791	0.800569	2.267139e-48	3.105014e-46
MsaG031473	MsaG032791	0.817122	6.754083e-52	1.378251e-49
MsaG018653	MsaG032791	0.805228	2.497465e-49	3.806431e-47
MsaG020694	MsaG032791	0.826711	4.159709e-54	1.097236e-51
MsaG026763	MsaG032791	0.815095	1.906615e-51	3.694426e-49
MsaG033645	MsaG032791	0.803716	5.142195e-49	7.567293e-47
MsaG039929	MsaG032791	0.801270	1.632529e-48	2.271448e-46
MsaG040596	MsaG032791	0.883300	1.078066e-70	2.274363e-67

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032791	MtrunA17_Chr5g0405211	31.818	154	85	6	5	145	9	155	3.55e-15	73.9
MsaG032791	MtrunA17_Chr8g0372121	33.548	155	81	6	5	145	7	153	6.35e-15	72.8
MsaG032791	MtrunA17_Chr4g0056541	32.903	155	82	6	5	145	8	154	7.65e-15	73.2
MsaG032791	MtrunA17_Chr4g0050701	27.957	186	98	8	5	165	12	186	7.95e-15	70.9
MsaG032791	MtrunA17_Chr7g0266701	28.161	174	101	7	1	152	1	172	1.40e-14	71.2
MsaG032791	MtrunA17_Chr8g0359921	35.766	137	70	7	5	133	15	141	1.69e-14	72.0
MsaG032791	MtrunA17_Chr2g0318101	31.618	136	81	4	5	134	16	145	3.13e-14	71.2
MsaG032791	MtrunA17_Chr2g0318081	34.058	138	73	7	5	134	17	144	2.16e-13	68.9
MsaG032791	MtrunA17_Chr4g0017921	30.460	174	100	8	5	165	9	174	2.72e-13	68.6
MsaG032791	MtrunA17_Chr3g0109821	30.000	160	82	8	5	145	7	155	1.03e-12	67.4
MsaG032791	MtrunA17_Chr3g0130731	33.577	137	72	8	5	133	8	133	1.06e-12	66.2
MsaG032791	MtrunA17_Chr2g0309891	30.657	137	81	6	5	134	7	136	7.44e-12	64.3
MsaG032791	MtrunA17_Chr1g0183741	32.026	153	83	9	5	145	11	154	8.26e-12	63.5
MsaG032791	MtrunA17_Chr6g0453131	30.827	133	77	6	6	131	17	141	1.19e-11	63.9
MsaG032791	MtrunA17_Chr3g0124761	32.353	136	75	6	5	133	8	133	1.58e-11	62.8
MsaG032791	MtrunA17_Chr8g0345691	28.846	156	87	7	1	142	9	154	2.20e-11	63.2
MsaG032791	MtrunA17_Chr7g0261411	32.680	153	82	9	5	145	11	154	2.60e-11	62.4
MsaG032791	MtrunA17_Chr1g0201021	32.593	135	73	6	6	131	21	146	2.77e-11	63.2
MsaG032791	MtrunA17_Chr4g0038751	29.530	149	90	6	6	145	17	159	5.04e-11	61.6
MsaG032791	MtrunA17_Chr5g0400461	26.452	155	95	6	5	144	7	157	5.04e-11	61.6
MsaG032791	MtrunA17_Chr4g0060931	29.375	160	89	8	1	145	15	165	5.16e-11	62.0
MsaG032791	MtrunA17_Chr8g0384351	29.310	174	97	9	5	174	11	162	6.49e-11	59.7
MsaG032791	MtrunA17_Chr2g0311661	32.192	146	82	7	2	137	19	157	9.35e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032791	AT4G36160.2	32.468	154	84	6	5	145	15	161	2.21e-16	78.2
MsaG032791	AT4G36160.1	32.468	154	84	6	5	145	11	157	2.25e-16	77.8
MsaG032791	AT4G36160.3	32.468	154	84	6	5	145	11	157	2.25e-16	77.8
MsaG032791	AT1G71930.2	29.677	155	93	5	5	148	10	159	1.18e-15	75.5
MsaG032791	AT1G71930.1	29.677	155	93	5	5	148	10	159	1.18e-15	75.5
MsaG032791	AT2G18060.3	31.818	154	85	6	5	145	10	156	1.29e-15	75.9
MsaG032791	AT2G18060.1	31.818	154	85	6	5	145	10	156	1.29e-15	75.9
MsaG032791	AT4G27410.2	31.677	161	85	8	5	150	15	165	1.99e-15	74.7
MsaG032791	AT4G27410.3	29.714	175	87	8	5	150	15	182	3.91e-14	71.2
MsaG032791	AT4G17980.1	30.435	138	80	4	5	133	7	137	1.28e-13	68.9
MsaG032791	AT4G17980.2	30.435	138	80	4	5	133	7	137	1.60e-13	68.9
MsaG032791	AT5G08790.1	28.972	214	117	11	5	196	8	208	2.51e-13	68.6
MsaG032791	AT3G15500.1	32.593	135	75	6	5	132	15	140	4.84e-13	67.8
MsaG032791	AT3G44290.2	25.874	143	93	5	6	142	16	151	5.27e-13	66.6
MsaG032791	AT5G63790.1	36.765	136	69	8	5	133	51	176	5.63e-13	67.8
MsaG032791	AT1G77450.1	36.496	137	68	7	5	133	11	136	5.66e-13	67.0
MsaG032791	AT3G44290.1	25.874	143	93	5	6	142	16	151	6.32e-13	67.8
MsaG032791	AT5G63790.2	36.765	136	69	8	5	133	61	186	6.72e-13	67.4
MsaG032791	AT1G52890.1	33.094	139	69	7	5	132	15	140	1.24e-12	66.6
MsaG032791	AT5G64530.2	33.077	130	69	5	5	131	4	118	1.36e-12	64.3
MsaG032791	AT5G64530.1	33.071	127	73	4	5	131	4	118	1.72e-12	64.7
MsaG032791	AT1G01720.1	33.577	137	72	6	5	133	8	133	2.15e-12	65.9
MsaG032791	AT5G17260.1	28.025	157	89	7	5	145	7	155	4.21e-12	65.9
MsaG032791	AT3G29035.1	32.090	134	75	6	5	131	25	149	5.35e-12	65.1
MsaG032791	AT3G03200.1	28.662	157	88	7	5	145	7	155	7.07e-12	65.1
MsaG032791	AT4G10350.1	31.210	157	84	8	5	145	10	158	7.16e-12	64.7
MsaG032791	AT4G28530.1	29.651	172	94	6	5	153	11	178	7.76e-12	64.7
MsaG032791	AT5G04395.1	31.293	147	81	6	5	134	29	172	7.97e-12	62.8
MsaG032791	AT5G50820.1	34.074	135	73	7	5	134	18	141	9.14e-12	62.8
MsaG032791	AT5G39610.1	30.573	157	84	8	5	145	21	168	9.14e-12	63.9
MsaG032791	AT1G54330.1	28.105	153	90	7	5	145	7	151	1.44e-11	63.5
MsaG032791	AT1G26870.1	29.231	130	80	4	6	129	18	141	1.83e-11	63.9
MsaG032791	AT5G64060.1	27.803	223	123	10	6	211	8	209	2.06e-11	63.5
MsaG032791	AT5G61430.1	29.697	165	92	8	1	150	14	169	2.34e-11	63.2
MsaG032791	AT5G07680.1	30.000	160	88	8	1	145	15	165	6.01e-11	62.0
MsaG032791	AT1G33280.1	29.870	154	87	7	5	145	9	154	6.11e-11	61.6
MsaG032791	AT5G07680.2	30.000	160	88	8	1	145	1	151	8.16e-11	61.6

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAACTTATGCTTGCAATTA+TGG	0.158474	6:+63295007	MsaT032791.1:CDS
ATGGATCTAGACTCTGAATT+TGG	0.204240	6:+63293830	MsaT032791.1:CDS
GGCATTGTAAGACTGGATTT+TGG	0.240614	6:+63293500	MsaT032791.1:CDS
CCATGGTTCTGACCGGATTA+AGG	0.270785	6:+63293366	MsaT032791.1:CDS
CCTCCTCGGCTCCTCAGTTT+TGG	0.293397	6:-63293479	None:intergenic
TGGATCTAGACTCTGAATTT+GGG	0.333596	6:+63293831	MsaT032791.1:CDS
CCTTGTGTGAGGGAAGAAAA+AGG	0.356880	6:-63293437	None:intergenic
TGTTGGAGCTGATGACGTTT+TGG	0.393392	6:+63293552	MsaT032791.1:CDS
AAAACACAGCAAACAACAAA+AGG	0.433446	6:-63293791	None:intergenic
TGAAGGAACTCAGCAACTTA+AGG	0.444240	6:+63293913	MsaT032791.1:CDS
ACAGTCTCAGCTCCTTAATC+CGG	0.483053	6:-63293378	None:intergenic
CCGAGGAGGCATTGTAAGAC+TGG	0.484776	6:+63293493	MsaT032791.1:CDS
TGAGCAAGATGTAATTCAAA+AGG	0.487325	6:-63293412	None:intergenic
ACTCAGCAACTTAAGGAGGC+TGG	0.487489	6:+63293920	MsaT032791.1:CDS
CTCAGCAACTTAAGGAGGCT+GGG	0.496847	6:+63293921	MsaT032791.1:CDS
GTTGGTGTTGGTGTTGAGCA+AGG	0.501020	6:+63293962	MsaT032791.1:CDS
GAGTCTAGATCCATCCCTCT+AGG	0.505901	6:-63293821	None:intergenic
GGCTACTCAAGCCACTCCTC+AGG	0.507680	6:+63294003	MsaT032791.1:CDS
TCTAGTGCAGGTGTTGGTGT+TGG	0.508728	6:+63293950	MsaT032791.1:CDS
GAAGAGGTTCAGAATTTCCA+TGG	0.526667	6:+63293349	MsaT032791.1:CDS
AGAACGCGATCAAAACTAGT+TGG	0.526855	6:+63293620	MsaT032791.1:CDS
GCTGTTAGAGACATTACTGT+TGG	0.528259	6:+63293535	MsaT032791.1:CDS
CACTCTTGTTAACAATCCTC+AGG	0.528444	6:+63293657	MsaT032791.1:CDS
TCTGATGACAAGGCGAATGA+AGG	0.528665	6:+63293896	MsaT032791.1:CDS
AGAATTTCCATGGTTCTGAC+CGG	0.533129	6:+63293359	MsaT032791.1:CDS
ACCTTCATCGACATCAGATA+AGG	0.533255	6:-63293855	None:intergenic
TTGTTCATCAGAAGGGAAGA+AGG	0.533280	6:-63293296	None:intergenic
GCAATGGATTTGCCTCCACC+TGG	0.541606	6:+63293268	None:intergenic
CCAGTCTTACAATGCCTCCT+CGG	0.543084	6:-63293493	None:intergenic
CCACATGGAAGCTATGACGC+CGG	0.546830	6:+63294983	MsaT032791.1:CDS
ATTGCAAGCATAAGTTGCTC+CGG	0.553025	6:-63295002	None:intergenic
AGGAGTGGCTTGAGTAGCCA+TGG	0.558328	6:-63293999	None:intergenic
AAGAAGGCAACACCAGGTGG+AGG	0.559720	6:-63293280	None:intergenic
CTAACAAGTTGTTCATCAGA+AGG	0.560217	6:-63293304	None:intergenic
GCTGGGACTAGTTCTAGTGC+AGG	0.570005	6:+63293938	MsaT032791.1:CDS
AGTCTAGATCCATCCCTCTA+GGG	0.570873	6:-63293820	None:intergenic
AGGTGATGCATCGCATTCCA+TGG	0.574376	6:+63293982	MsaT032791.1:CDS
CAGTAAAACTCCCTTGTGTG+AGG	0.577220	6:-63293448	None:intergenic
CCTTAATCCGGTCAGAACCA+TGG	0.580530	6:-63293366	None:intergenic
TAACAAGTTGTTCATCAGAA+GGG	0.581916	6:-63293303	None:intergenic
ACTAGTTCTAGTGCAGGTGT+TGG	0.583083	6:+63293944	MsaT032791.1:CDS
GAAGGGAAGAAGGCAACACC+AGG	0.588550	6:-63293286	None:intergenic
GCCTTATCTGATGTCGATGA+AGG	0.589295	6:+63293854	MsaT032791.1:CDS
AGTAAAACTCCCTTGTGTGA+GGG	0.603789	6:-63293447	None:intergenic
GTAATTCAAAAGGATCATAC+CGG	0.608058	6:-63293402	None:intergenic
TTAAGGAGCTGAGACTGTAC+CGG	0.611881	6:+63293383	MsaT032791.1:CDS
GGGAAGAAGGCAACACCAGG+TGG	0.613301	6:-63293283	None:intergenic
CTGCCAAAACTGAGGAGCCG+AGG	0.619650	6:+63293476	MsaT032791.1:CDS
CCGGCGTCATAGCTTCCATG+TGG	0.629672	6:-63294983	None:intergenic
TGACTATGTTGTGTCCCACA+TGG	0.631632	6:+63294968	MsaT032791.1:CDS
GGATCTAGACTCTGAATTTG+GGG	0.633562	6:+63293832	MsaT032791.1:CDS
CCAAAACTGAGGAGCCGAGG+AGG	0.634705	6:+63293479	MsaT032791.1:CDS
CGGCGTCATAGCTTCCATGT+GGG	0.637767	6:-63294982	None:intergenic
CTGCTTCACTGCCAAAACTG+AGG	0.647730	6:+63293468	MsaT032791.1:CDS
GATGAAGGTGATGTTGCTGA+TGG	0.651079	6:+63293869	MsaT032791.1:CDS
AGGAACTCAGCAACTTAAGG+AGG	0.651240	6:+63293916	MsaT032791.1:CDS
TGATGGCGACTCTGATGACA+AGG	0.669475	6:+63293886	MsaT032791.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	63293271	63295049	63293271	ID=MsaG032791
Chr6	mRNA	63293271	63295049	63293271	ID=MsaT032791.1;Parent=MsaG032791
Chr6	exon	63293271	63293678	63293271	ID=MsaT032791.1.exon1;Parent=MsaT032791.1
Chr6	CDS	63293271	63293678	63293271	ID=cds.MsaT032791.1;Parent=MsaT032791.1
Chr6	exon	63293788	63294024	63293788	ID=MsaT032791.1.exon2;Parent=MsaT032791.1
Chr6	CDS	63293788	63294024	63293788	ID=cds.MsaT032791.1;Parent=MsaT032791.1
Chr6	exon	63294948	63295049	63294948	ID=MsaT032791.1.exon3;Parent=MsaT032791.1
Chr6	CDS	63294948	63295049	63294948	ID=cds.MsaT032791.1;Parent=MsaT032791.1

Gene Sequence

>MsaT032791.1

ATGGATTTGCCTCCACCTGGTGTTGCCTTCTTCCCTTCTGATGAACAACTTGTTAGCTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCCATGGTTCTGACCGGATTAAGGAGCTGAGACTGTACCGGTATGATCCTTTTGAATTACATCTTGCTCAGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAACTGAGGAGCCGAGGAGGCATTGTAAGACTGGATTTTGGATGCAGAAGGGAGCTGTTAGAGACATTACTGTTGGAGCTGATGACGTTTTGGTGGGAAAGAAGTCTCTCTTCATTTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGTTAACAATCCTCAGGCTCCTTTTGTTGTTTGCTGTGTTTTTGACTGCCCTAGAGGGATGGATCTAGACTCTGAATTTGGGGCCTTATCTGATGTCGATGAAGGTGATGTTGCTGATGGCGACTCTGATGACAAGGCGAATGAAGGAACTCAGCAACTTAAGGAGGCTGGGACTAGTTCTAGTGCAGGTGTTGGTGTTGGTGTTGAGCAAGGTGATGCATCGCATTCCATGGCTACTCAAGCCACTCCTCAGGCGAGTTATACTGCATTTCTTGACTATGTTGTGTCCCACATGGAAGCTATGACGCCGGAGCAACTTATGCTTGCAATTATGGATGAGAAAGATTTTATTTAA

Protein sequence

>MsaT032791.1

MDLPPPGVAFFPSDEQLVSYLANKNNEEVQNFHGSDRIKELRLYRYDPFELHLAQSFFFPHTREFYCFTAKTEEPRRHCKTGFWMQKGAVRDITVGADDVLVGKKSLFIFYIGNSPQNAIKTSWTMYEYTLVNNPQAPFVVCCVFDCPRGMDLDSEFGALSDVDEGDVADGDSDDKANEGTQQLKEAGTSSSAGVGVGVEQGDASHSMATQATPQASYTAFLDYVVSHMEAMTPEQLMLAIMDEKDFI*