AlfalfaGEDB | MsaG033044

M. sativa ssp. caerulea / MsaG033044

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033044	KEH26142.1	78.535	396	45	4	1	365	1	387	0.0	610

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033044	sp\|Q8L9Y3\|ARR14_ARATH	38.996	259	121	5	13	234	6	264	1.69e-50	176

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033044	tr\|A0A072U908\|A0A072U908_MEDTR	78.535	396	45	4	1	365	1	387	0.0	610

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TFs/TRs:

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Gene ID	Type	Classification
MsaG033044	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003444	MsaG033044	0.804691	3.230050e-49	4.861818e-47
MsaG017317	MsaG033044	0.806058	1.675348e-49	2.603477e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033044	MtrunA17_Chr6g0471041	81.967	366	48	3	1	357	1	357	0.0	601
MsaG033044	MtrunA17_Chr6g0471041	62.000	100	30	1	266	365	326	417	3.82e-28	114
MsaG033044	MtrunA17_Chr4g0009851	45.981	311	144	7	1	298	1	300	4.60e-72	227
MsaG033044	MtrunA17_Chr4g0009901	46.639	238	122	3	14	249	10	244	6.59e-62	199
MsaG033044	MtrunA17_Chr8g0383961	48.425	254	103	5	1	254	1	226	1.66e-60	196
MsaG033044	MtrunA17_Chr1g0161431	43.580	257	113	4	13	237	10	266	8.80e-60	203
MsaG033044	MtrunA17_Chr4g0009981	42.045	264	126	5	59	302	2	258	1.76e-53	177
MsaG033044	MtrunA17_Chr4g0067981	38.735	253	126	3	13	237	30	281	6.84e-50	177
MsaG033044	MtrunA17_Chr2g0294911	39.113	248	124	3	13	234	28	274	2.49e-49	175
MsaG033044	MtrunA17_Chr8g0376881	38.843	242	121	3	14	228	25	266	2.23e-48	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033044	AT2G01760.1	38.996	259	121	5	13	234	6	264	1.72e-51	176
MsaG033044	AT2G01760.2	35.192	287	121	6	13	234	6	292	1.70e-45	161
MsaG033044	AT1G67710.1	40.239	251	118	6	17	236	10	259	1.74e-45	163
MsaG033044	AT3G16857.1	34.909	275	130	6	8	234	27	300	1.78e-43	160
MsaG033044	AT3G16857.2	34.909	275	130	6	8	234	27	300	2.08e-43	160
MsaG033044	AT2G25180.1	34.109	258	144	4	1	233	1	257	2.47e-38	145
MsaG033044	AT4G31920.1	35.317	252	148	5	1	238	1	251	1.00e-37	142
MsaG033044	AT3G16857.3	33.913	230	105	4	52	234	1	230	2.06e-35	137
MsaG033044	AT2G01760.3	38.265	196	84	5	76	234	4	199	1.06e-34	130
MsaG033044	AT5G58080.1	33.740	246	138	4	13	234	13	257	2.15e-34	134

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Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGCATTAGAGCATG+TGG	0.613637	6:-74459113	None:intergenic
AAAAGAATCTCATGTTGATG+TGG	0.621750	6:+74459704	MsaT033044.1:CDS
AAACCCTCTAAGAATTGTTG+GGG	0.603847	6:-74458885	None:intergenic
AACAATTCTTAGAGGGTTTA+AGG	0.118099	6:+74458889	MsaT033044.1:CDS
AACGATGGCATTGTGGATAG+TGG	0.451069	6:-74458934	None:intergenic
AATGCATCAAGAATTTCTAA+GGG	0.619626	6:+74459785	MsaT033044.1:CDS
AATGGCTCTTTCAACTGAGA+TGG	0.494149	6:+74458854	None:intergenic
AATTGAGACTTACTTTAAAG+AGG	0.462807	6:+74461165	MsaT033044.1:CDS
AATTTGTTGGAGAAAATCAT+AGG	0.440238	6:-74459212	None:intergenic
ACAATTCTTAGAGGGTTTAA+GGG	0.385698	6:+74458890	MsaT033044.1:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	74458855	74461601	74458855	ID=MsaG033044
Chr6	mRNA	74458855	74461601	74458855	ID=MsaT033044.1;Parent=MsaG033044
Chr6	exon	74458855	74458984	74458855	ID=MsaT033044.1.exon1;Parent=MsaT033044.1
Chr6	CDS	74458855	74458984	74458855	ID=cds.MsaT033044.1;Parent=MsaT033044.1
Chr6	exon	74459110	74459262	74459110	ID=MsaT033044.1.exon2;Parent=MsaT033044.1
Chr6	CDS	74459110	74459262	74459110	ID=cds.MsaT033044.1;Parent=MsaT033044.1
Chr6	exon	74459532	74459831	74459532	ID=MsaT033044.1.exon3;Parent=MsaT033044.1
Chr6	CDS	74459532	74459831	74459532	ID=cds.MsaT033044.1;Parent=MsaT033044.1
Chr6	exon	74459925	74460001	74459925	ID=MsaT033044.1.exon4;Parent=MsaT033044.1
Chr6	CDS	74459925	74460001	74459925	ID=cds.MsaT033044.1;Parent=MsaT033044.1
Chr6	exon	74461164	74461601	74461164	ID=MsaT033044.1.exon5;Parent=MsaT033044.1
Chr6	CDS	74461164	74461601	74461164	ID=cds.MsaT033044.1;Parent=MsaT033044.1

Gene Sequence

>MsaT033044.1

ATGGCTCTTTCAACTGAGATGGAAGCACTTCCCCAACAATTCTTAGAGGGTTTAAGGGTTCTTGTGATTGATCATGACACCACTATCCACAATGCCATCGTTGAAAAGTCAATTGGATGGAATTACAAAGTTACCACATGCTCTAATGCTTCTTTTGCATTGACTCTTTTGAGGGAAGCAAAGGGTTGTTTTGATGTGATACTAATTGAAGAACTAATCTCAGACATGAATTCCTATGATTTTCTCCAACAAATTACCCAACAAATCAATATTCCTGTCATTATGATGGGTGTTGATGGATCAACAAGTGCATCCATGAAGGCTATTGCAAACGGGGCTTGTGAGTATTGTGTTAAGCCTTTGAGTGATGACCTAATCAAAAACATGTGCAAACACGTTCCAAGGAAAACTTTGAATGAAACTAAACATGATCAAATTCATGTTGATAATGGCACAAAAGAATCTCATGTTGATGTGGTTGAAAAGGATAATTATCAACCCCCAACAAAAAAGAACCGTTTAACATGGTCTCAAGCAATGCATCAAGAATTTCTAAGGGCTGTGAATCAATTTGGCCTTGATAATGCAAAACCAAAAAAAATTATTCAAGTGATGAATGTTCCTGGTTTGACAAGAGAGCATATTGCTAGCCATTTACAGAAATTGAGACTTACTTTAAAGAGGTCAAATGAAGTGACACAGTTAAAGAATGAAATACCAAAAGGTGAGTGGAAAAAATCAAAGCAGGATCAATGTCATCTTGAAGCTGCTAAGTCAACGCCAGAACTTGATCAAAATGTTAAAAATCCAGTCATACAATGTGACAATAATTCTCATGCTCCACAACATTCATCAAAATTGGCCAACATTTTTCCTGATCAGTCTAATGTTTTGAATTCGATCATACAATATGACAATAACTATCAAGCACTACAACATTCACTGACATTTACCAACAATTTTACTGATCAGTCTAATGTTATGAATTCAGTCATACAACATGACAATAATTCTCATGCACCACAACATTCACCGACATTCACCAATATTTTTACTGATCAGTCCAACATGAATCCAGGTGATGAGTTCTTTAATTGA

Protein sequence

>MsaT033044.1

MALSTEMEALPQQFLEGLRVLVIDHDTTIHNAIVEKSIGWNYKVTTCSNASFALTLLREAKGCFDVILIEELISDMNSYDFLQQITQQINIPVIMMGVDGSTSASMKAIANGACEYCVKPLSDDLIKNMCKHVPRKTLNETKHDQIHVDNGTKESHVDVVEKDNYQPPTKKNRLTWSQAMHQEFLRAVNQFGLDNAKPKKIIQVMNVPGLTREHIASHLQKLRLTLKRSNEVTQLKNEIPKGEWKKSKQDQCHLEAAKSTPELDQNVKNPVIQCDNNSHAPQHSSKLANIFPDQSNVLNSIIQYDNNYQALQHSLTFTNNFTDQSNVMNSVIQHDNNSHAPQHSPTFTNIFTDQSNMNPGDEFFN*