AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033189

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033189 MtrunA17_Chr6g0472471 93.888 769 35 1 1 757 1 769 0.0 1435
MsaG033189 MtrunA17_Chr1g0203671 57.087 769 293 13 11 755 36 791 0.0 860
MsaG033189 MtrunA17_Chr7g0246631 51.729 781 311 18 6 753 27 774 0.0 785
MsaG033189 MtrunA17_Chr8g0350321 53.699 730 310 11 34 753 95 806 0.0 769
MsaG033189 MtrunA17_Chr2g0322731 44.693 716 347 12 47 753 90 765 0.0 600
MsaG033189 MtrunA17_Chr4g0024351 44.309 738 361 14 26 753 32 729 0.0 600
MsaG033189 MtrunA17_Chr5g0410711 40.836 742 403 16 26 753 59 778 0.0 546
MsaG033189 MtrunA17_Chr6g0481041 41.935 713 382 14 56 753 28 723 0.0 541
MsaG033189 MtrunA17_Chr2g0331581 38.810 706 360 11 61 753 143 789 4.67e-170 509
MsaG033189 MtrunA17_Chr5g0394111 34.708 703 350 12 64 753 40 646 2.47e-133 409
MsaG033189 MtrunA17_Chr7g0224451 36.970 495 236 11 56 542 15 441 2.70e-100 317
MsaG033189 MtrunA17_Chr2g0292731 31.273 534 328 12 239 753 35 548 1.58e-86 284
MsaG033189 MtrunA17_Chr2g0292741 31.698 530 326 11 239 753 27 535 3.91e-85 280
MsaG033189 MtrunA17_Chr7g0224481 36.154 390 177 9 56 438 15 339 6.34e-72 238
MsaG033189 MtrunA17_Chr2g0292761 29.635 631 336 22 138 753 3 540 1.53e-70 241
MsaG033189 MtrunA17_Chr7g0224491 40.694 317 160 11 441 753 1 293 1.75e-56 195
MsaG033189 MtrunA17_Chr5g0419821 48.819 127 65 0 51 177 28 154 1.19e-37 138
MsaG033189 MtrunA17_Chr2g0319671 30.502 259 139 4 264 489 18 268 8.53e-34 132
MsaG033189 MtrunA17_Chr1g0205981 35.047 214 110 7 112 322 1 188 4.76e-26 106
MsaG033189 MtrunA17_Chr2g0319701 28.270 237 156 4 519 754 1 224 5.40e-24 101
MsaG033189 MtrunA17_Chr2g0319681 28.111 217 132 4 544 753 26 225 7.42e-22 95.5
MsaG033189 MtrunA17_Chr2g0319691 41.667 108 59 1 377 484 1 104 9.77e-22 92.4
MsaG033189 MtrunA17_Chr7g0224461 43.333 120 59 3 636 753 2 114 3.02e-16 75.5
MsaG033189 MtrunA17_Chr2g0304191 30.827 133 87 2 272 401 1 131 1.12e-15 74.3
MsaG033189 MtrunA17_Chr1g0179581 38.202 89 55 0 54 142 22 110 1.45e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033189 AT3G61150.1 50.137 732 334 13 35 753 90 803 0.0 713
MsaG033189 AT4G00730.1 51.377 726 306 13 34 753 114 798 0.0 710
MsaG033189 AT5G52170.3 47.283 736 311 15 23 757 23 682 0.0 631
MsaG033189 AT5G52170.1 47.283 736 311 15 23 757 23 682 0.0 631
MsaG033189 AT5G52170.2 46.892 740 313 15 23 757 23 687 0.0 627
MsaG033189 AT4G04890.2 44.782 757 341 16 29 753 24 735 0.0 621
MsaG033189 AT4G04890.1 44.782 757 341 16 29 753 24 735 0.0 621
MsaG033189 AT4G21750.2 44.882 762 357 14 29 754 24 758 0.0 615
MsaG033189 AT4G21750.1 44.882 762 357 14 29 754 24 758 0.0 615
MsaG033189 AT4G21750.3 44.882 762 357 14 29 754 24 758 0.0 615
MsaG033189 AT4G21750.4 44.882 762 357 14 29 754 24 758 0.0 615
MsaG033189 AT1G05230.8 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.4 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.9 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.10 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.7 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.11 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.2 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.6 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.5 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.1 42.686 752 366 13 12 753 20 716 0.0 588
MsaG033189 AT1G05230.3 42.420 752 366 13 12 753 20 714 0.0 582
MsaG033189 AT1G73360.1 41.160 707 383 14 56 753 31 713 0.0 537
MsaG033189 AT4G00730.2 53.333 465 190 7 34 498 114 551 8.13e-169 498
MsaG033189 AT1G05230.12 43.569 622 303 11 12 623 20 603 2.04e-168 499
MsaG033189 AT1G17920.2 37.517 725 397 14 40 753 2 681 1.63e-166 496
MsaG033189 AT1G17920.1 37.517 725 397 14 40 753 2 681 1.63e-166 496
MsaG033189 AT1G79840.3 37.902 715 359 14 56 755 42 686 2.67e-158 475
MsaG033189 AT1G79840.6 37.902 715 359 14 56 755 42 686 2.67e-158 475
MsaG033189 AT1G79840.1 37.902 715 359 14 56 755 100 744 1.39e-157 476
MsaG033189 AT2G32370.1 36.265 739 395 10 26 751 43 718 2.65e-157 474
MsaG033189 AT1G79840.2 37.902 715 359 14 56 755 129 773 4.48e-157 475
MsaG033189 AT5G46880.3 36.176 727 419 15 50 754 104 807 2.33e-143 441
MsaG033189 AT5G46880.1 36.176 727 419 15 50 754 104 807 2.33e-143 441
MsaG033189 AT5G46880.2 36.176 727 419 15 50 754 104 807 2.33e-143 441
MsaG033189 AT4G25530.1 36.200 721 383 15 44 753 28 682 3.31e-143 436
MsaG033189 AT4G17710.1 36.072 718 354 17 48 753 79 703 1.24e-132 410
MsaG033189 AT1G79840.4 37.651 579 288 12 56 619 100 620 1.51e-126 391
MsaG033189 AT3G03260.1 32.060 733 407 17 54 753 20 694 7.84e-119 374
MsaG033189 AT5G17320.1 32.661 744 440 24 36 753 7 715 1.65e-110 352
MsaG033189 AT1G79840.5 38.085 449 214 9 56 496 100 492 5.14e-96 308
MsaG033189 AT1G34650.1 30.704 710 445 19 64 753 23 705 5.14e-93 306
MsaG033189 AT3G03260.2 31.227 554 326 11 54 589 20 536 1.85e-88 289
MsaG033189 AT5G07260.2 25.220 567 330 20 122 683 9 486 1.83e-38 151
MsaG033189 AT5G07260.1 25.132 569 333 20 122 683 9 491 4.66e-38 150

Find 213 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAGTTCGAAGCCAGAATT+TGG 0.202187 6:+79000415 None:intergenic
GCTTCCTTCTGGCTGTATTT+TGG 0.205155 6:-78999522 MsaT033189.1:CDS
TTCTTCTTCTTCTTGCTCTT+TGG 0.214124 6:+79001604 None:intergenic
TCCTCTCTGGATTAAGAGTT+TGG 0.239974 6:-79000173 MsaT033189.1:CDS
ATTGGAGAAAACTTTGATTC+TGG 0.275478 6:-79001726 MsaT033189.1:CDS
TTTAACGATCCGAAACAAAA+TGG 0.310819 6:-79000097 MsaT033189.1:CDS
AATGTTGGAATGGGACTTAT+TGG 0.311960 6:-79001744 MsaT033189.1:CDS
TGCCAGACTGAAAGAGGAAT+TGG 0.317670 6:-79000512 MsaT033189.1:CDS
AGCAGCATGTCGGAGGCATT+TGG 0.324222 6:-78999616 MsaT033189.1:CDS
CGAACAAGCAAGTCAAGTTC+TGG 0.326569 6:-79001133 MsaT033189.1:CDS
GGACAACACTCTCATCATAT+TGG 0.340934 6:+78998899 None:intergenic
TCGAAGCCAGAATTTGGATT+TGG 0.350110 6:+79000421 None:intergenic
CCAAATTCTGGCTTCGAACT+TGG 0.355973 6:-79000415 MsaT033189.1:CDS
ACTATGCCAAATCCAAATTC+TGG 0.358829 6:-79000427 MsaT033189.1:CDS
TATTGTCGAACCGGCACTAA+TGG 0.359494 6:+78999659 None:intergenic
TGCGGTGGCCCAGCAATTCC+TGG 0.366906 6:-79000574 MsaT033189.1:CDS
GCTATGCTTATTGATCTTGC+AGG 0.368676 6:-79000238 MsaT033189.1:CDS
TATCGGTCCATGAGTCTTGC+AGG 0.370886 6:+78997802 None:intergenic
ATGTCGCTGCCGATGGGATT+TGG 0.374806 6:-79000352 MsaT033189.1:CDS
GGTGGATGCTTGCTGACATT+TGG 0.378763 6:-78997585 MsaT033189.1:CDS
TATCGTCCATTGGTTAATTC+CGG 0.379223 6:-78998868 MsaT033189.1:CDS
GCACACTCCTCAACAAATTC+AGG 0.380093 6:-79001572 MsaT033189.1:CDS
TCAGTTCTTTGCTTCTCATC+AGG 0.380799 6:+79001179 None:intergenic
GTTAATTCCGGCAATGCATT+TGG 0.384755 6:-78998856 MsaT033189.1:CDS
TTCTGGCTTCGAACTTGGTA+TGG 0.384994 6:-79000410 MsaT033189.1:CDS
ATTTGGATTTGGCATAGTTA+TGG 0.388515 6:+79000432 None:intergenic
AGCTGTAAGAAGCTTCCTTC+TGG 0.391319 6:-78999533 MsaT033189.1:CDS
GAAAATTGTCCAGGAATTGC+TGG 0.395809 6:+79000565 None:intergenic
ACCTTGACCCACAAGTTCTT+CGG 0.397052 6:-79000478 MsaT033189.1:CDS
ACTGTCGCCCATGTTACTAA+TGG 0.400218 6:+78998418 None:intergenic
GGTATGGGAGACGGAGTTAT+GGG 0.401823 6:-79000331 MsaT033189.1:CDS
AACTTTGATTCTGGTCTGCT+TGG 0.408156 6:-79001717 MsaT033189.1:CDS
ATGAGCACCAAATGCATTGC+CGG 0.408651 6:+78998849 None:intergenic
GACAACACTCTCATCATATT+GGG 0.408834 6:+78998900 None:intergenic
TTACAATGTTGCAGGTTACT+TGG 0.408841 6:-78998933 MsaT033189.1:intron
GTCGGCCGTAATGCTGCTAA+TGG 0.409360 6:-78999569 MsaT033189.1:CDS
GCAGCATGTCGGAGGCATTT+GGG 0.409865 6:-78999615 MsaT033189.1:CDS
TGTTTCCATTAGCAGCATTA+CGG 0.413443 6:+78999564 None:intergenic
TTTCTTGAATTAGACGCAAC+TGG 0.414309 6:-79000695 MsaT033189.1:intron
TGGTATGGGAGACGGAGTTA+TGG 0.416058 6:-79000332 MsaT033189.1:CDS
AGGAAAATGGAAGGAAATGT+TGG 0.420660 6:-79001759 MsaT033189.1:CDS
CTCAGCCGGATTCTCCTCTC+TGG 0.420917 6:-79000186 MsaT033189.1:CDS
CACCGGAGGACCACCAGCTT+AGG 0.422085 6:-79000543 MsaT033189.1:CDS
GATCGGAAATAGTGCTCAAC+AGG 0.422993 6:-79000266 MsaT033189.1:CDS
CGATATTGTTGACGTTTGAA+TGG 0.425015 6:+78997658 None:intergenic
ACTGAAGCTACAAGACAAAC+TGG 0.426102 6:-79000067 MsaT033189.1:CDS
TGCATCTTTGATTCTCTGAA+TGG 0.427477 6:+78997479 None:intergenic
GGAGCTTCGGCGCTACAATC+TGG 0.428974 6:-79000310 MsaT033189.1:CDS
GTTGACACTAAGAATAGAAA+TGG 0.431047 6:+78998187 None:intergenic
ACCAATCGATTCACAATCCT+TGG 0.431465 6:-78997754 MsaT033189.1:CDS
AGCGACATGTTGAAATTGCT+TGG 0.435381 6:+79000367 None:intergenic
TTGATTCTGGTCTGCTTGGA+AGG 0.437279 6:-79001713 MsaT033189.1:intron
TCTTAGCAAGAAGCTTGGTT+TGG 0.442737 6:-79001156 MsaT033189.1:CDS
ATGCATTTGGTGCTCATCGA+TGG 0.444476 6:-78998843 MsaT033189.1:CDS
ATGTTTCCATGTATGATTGC+TGG 0.445315 6:-78999859 MsaT033189.1:CDS
CAGTGGTGGAGAGTCTTCAT+TGG 0.446749 6:-78997721 MsaT033189.1:CDS
CTGTCGCCCATGTTACTAAT+GGG 0.449407 6:+78998419 None:intergenic
AGAAGAAGAAGAAATATCAT+AGG 0.449873 6:-79001594 MsaT033189.1:CDS
AGAGGATGTCGGATTACTTC+TGG 0.459296 6:-78998484 MsaT033189.1:CDS
GCCCAATTCCTCTTTCAGTC+TGG 0.459513 6:+79000510 None:intergenic
GTCATATCTAGTGGAATGAA+TGG 0.463447 6:-78999820 MsaT033189.1:CDS
TTCGACAATATAGCTTCCTC+AGG 0.463726 6:-78999646 MsaT033189.1:CDS
CGAGGGAATCACACTTCCTC+TGG 0.467613 6:+78998809 None:intergenic
TGATCTTGCAGGAGCTGCTA+TGG 0.468204 6:-79000227 MsaT033189.1:CDS
ACCGGAGGACCACCAGCTTA+GGG 0.470424 6:-79000542 MsaT033189.1:CDS
GGATTCCGTTGCCTCGGCAA+AGG 0.472456 6:-78998322 MsaT033189.1:CDS
CTTTGCTTCTCATCAGGATG+TGG 0.472677 6:+79001185 None:intergenic
TGTCGGAGGCATTTGGGTTG+TGG 0.474116 6:-78999609 MsaT033189.1:CDS
TTAATCCAGAGAGGAGAATC+CGG 0.476039 6:+79000181 None:intergenic
TTGACGTTTGAATGGCCGTC+CGG 0.476807 6:+78997666 None:intergenic
GTCCTTCGAGTGCGTGCAAG+TGG 0.478216 6:-78998451 MsaT033189.1:CDS
ATGGGAAGGAATGGTGTTGT+TGG 0.478257 6:-79000391 MsaT033189.1:CDS
AGCTTGTCCTTTAGCAATGT+GGG 0.478850 6:+78998220 None:intergenic
ACCCTAAGCTGGTGGTCCTC+CGG 0.481325 6:+79000541 None:intergenic
GATTCCGTTGCCTCGGCAAA+GGG 0.482104 6:-78998321 MsaT033189.1:CDS
GATTGCTGGAGCTTCAACCT+TGG 0.482566 6:-78999845 MsaT033189.1:CDS
ATTGCTAAAGGACAAGCTCT+TGG 0.483173 6:-78998215 MsaT033189.1:CDS
ATGTGGGCCACCTCCTGCAT+TGG 0.484739 6:+78998236 None:intergenic
TACAATGTTGCAGGTTACTT+GGG 0.486862 6:-78998932 MsaT033189.1:intron
CGGTAGCGGCGGCAATGGCG+AGG 0.488679 6:-78997619 MsaT033189.1:CDS
TTTCTTACCATGTGGAGTCA+TGG 0.491235 6:+78998526 None:intergenic
GACCGATAGCTCTGGCTCAA+TGG 0.491819 6:-78997787 MsaT033189.1:CDS
AGACGGAGTTATGGGAGCTT+CGG 0.494120 6:-79000323 MsaT033189.1:CDS
ATGTCGGATTACTTCTGGTC+GGG 0.495633 6:-78998479 MsaT033189.1:CDS
ATGGAAGGAAATGTTGGAAT+GGG 0.496065 6:-79001753 MsaT033189.1:CDS
CTCTGGATTAAGAGTTTGGA+TGG 0.496804 6:-79000169 MsaT033189.1:CDS
AGGAAGCTATATTGTCGAAC+CGG 0.497244 6:+78999650 None:intergenic
CTCCTGCATTGGTCCACCAT+TGG 0.497322 6:+78998247 None:intergenic
ACTGATCTTAGCAAGAAGCT+TGG 0.500826 6:-79001161 MsaT033189.1:CDS
GCTGCCGATGGGATTTGGTA+TGG 0.501037 6:-79000347 MsaT033189.1:CDS
GTCTATACCTGCAAGACTCA+TGG 0.502681 6:-78997809 MsaT033189.1:CDS
TTTCAACATGTCGCTGCCGA+TGG 0.503516 6:-79000359 MsaT033189.1:CDS
TCCTCCGGTGAAAATTGTCC+AGG 0.504749 6:+79000556 None:intergenic
TGAGAATATGATTCTGAGGC+AGG 0.505324 6:-79000665 MsaT033189.1:CDS
GCCAGACTGAAAGAGGAATT+GGG 0.506100 6:-79000511 MsaT033189.1:CDS
GGTAGCGGCGGCAATGGCGA+GGG 0.507624 6:-78997618 MsaT033189.1:CDS
GTCCAATGGTGGACCAATGC+AGG 0.512521 6:-78998249 MsaT033189.1:CDS
ATGGAGGCAAATGCCGAGAC+TGG 0.514571 6:+79000451 None:intergenic
TGAAAATGCCAGACTGAAAG+AGG 0.521331 6:-79000518 MsaT033189.1:CDS
TCTGGCTTCGAACTTGGTAT+GGG 0.522216 6:-79000409 MsaT033189.1:CDS
TGGTGGACCAATGCAGGAGG+TGG 0.523454 6:-78998243 MsaT033189.1:CDS
CTGCCGATGGGATTTGGTAT+GGG 0.523570 6:-79000346 MsaT033189.1:CDS
TGCATTTGGTGCTCATCGAT+GGG 0.525055 6:-78998842 MsaT033189.1:CDS
ATCTGGATTGCAATCACCAA+TGG 0.526445 6:-79000293 MsaT033189.1:CDS
CGCCTCTCATCTGTGCCTCA+CGG 0.527454 6:+78998285 None:intergenic
AGTGACGGTAGCGGCGGCAA+TGG 0.531829 6:-78997624 MsaT033189.1:CDS
CCAATGGACGATAGGCTTGG+TGG 0.532530 6:+78998878 None:intergenic
AATGGAAGGAAATGTTGGAA+TGG 0.532593 6:-79001754 MsaT033189.1:CDS
GTTTAGGCTGTGAATCCGAC+TGG 0.533808 6:-78997846 MsaT033189.1:intron
TCAACAAACTGTTCTCAGCT+CGG 0.535021 6:+79000629 None:intergenic
TCTGGATTGCAATCACCAAT+GGG 0.536322 6:-79000292 MsaT033189.1:CDS
CAGAGAATCAAAGATGCACT+TGG 0.539207 6:-78997474 MsaT033189.1:CDS
ACGATGCTAGAAGTGGCATC+TGG 0.540329 6:+78998368 None:intergenic
AATCAACAGTGCAGGTCTTG+TGG 0.541045 6:-79000035 MsaT033189.1:CDS
AACACCCTTTGCCGAGGCAA+CGG 0.542039 6:+78998317 None:intergenic
CAATCACCAATGGGAATGAT+CGG 0.542363 6:-79000283 MsaT033189.1:CDS
GAAAAGCCAGAAGGTAAAAG+AGG 0.543396 6:+78997696 None:intergenic
GACCATTGAGCCAGAGCTAT+CGG 0.544879 6:+78997785 None:intergenic
TCGAGTTCCTGAATTTGTTG+AGG 0.546869 6:+79001565 None:intergenic
AATGGCGAGGGCGGAGACAA+TGG 0.548601 6:-78997606 MsaT033189.1:CDS
CTGTGTGGATTCCGTTGCCT+CGG 0.548818 6:-78998328 MsaT033189.1:CDS
GCCGAAGAACTTGTGGGTCA+AGG 0.548931 6:+79000477 None:intergenic
ACCTTGGATGTCATATCTAG+TGG 0.549602 6:-78999829 MsaT033189.1:CDS
TTGCAGAACAGGAAAATGGA+AGG 0.550574 6:-79001768 MsaT033189.1:intron
GTTCTTCGGCCGACCAGTCT+CGG 0.550974 6:-79000464 MsaT033189.1:CDS
GACTCATGGACCGATAGCTC+TGG 0.552145 6:-78997795 MsaT033189.1:CDS
ACTAATCAATATGTCTCAGC+CGG 0.552787 6:-79000200 MsaT033189.1:CDS
CCACCAAGCCTATCGTCCAT+TGG 0.553933 6:-78998878 MsaT033189.1:CDS
CTCCGTGAGGCACAGATGAG+AGG 0.554603 6:-78998287 MsaT033189.1:CDS
TGGAGAAGGCACTTGAACCT+CGG 0.554900 6:+78999679 None:intergenic
CGCCCATGTTACTAATGGGA+AGG 0.556790 6:+78998423 None:intergenic
AGCGGCGGCAATGGCGAGGG+CGG 0.557775 6:-78997615 MsaT033189.1:CDS
TTCAACATGTCGCTGCCGAT+GGG 0.557834 6:-79000358 MsaT033189.1:CDS
TCCTTCGAGTGCGTGCAAGT+GGG 0.557945 6:-78998450 MsaT033189.1:CDS
TCAAACGTCAACAATATCGT+TGG 0.558105 6:-78997654 MsaT033189.1:CDS
AGTGCCTTCTCCATTAGTGC+CGG 0.558202 6:-78999669 MsaT033189.1:CDS
GAGGTGGCCCACATTGCTAA+AGG 0.558752 6:-78998227 MsaT033189.1:CDS
CTATTTCCGATCATTCCCAT+TGG 0.561989 6:+79000277 None:intergenic
GTGACATGAAAATCATGTAT+CGG 0.563427 6:-78998397 MsaT033189.1:CDS
TGGATTTGGCATAGTTATGG+AGG 0.563900 6:+79000435 None:intergenic
TCCCACTTGCACGCACTCGA+AGG 0.564609 6:+78998449 None:intergenic
CTGGTCGGCCGAAGAACTTG+TGG 0.566405 6:+79000470 None:intergenic
TGTGGAAACGATGTTGGACG+CGG 0.568028 6:-79000017 MsaT033189.1:intron
GCCTCAGAATCATATTCTCA+TGG 0.568131 6:+79000668 None:intergenic
TAACCAATGGACGATAGGCT+TGG 0.569894 6:+78998875 None:intergenic
CTGCTTCCCTCGTTTCCAGT+CGG 0.570440 6:+78997831 None:intergenic
CACTTGCAAAGATCCACTCC+TGG 0.570849 6:+78999795 None:intergenic
TCCTTGGACATCGTGATCAG+TGG 0.574710 6:-78997738 MsaT033189.1:CDS
TTTATGTCAATAGGATCAGT+GGG 0.577370 6:-78999887 MsaT033189.1:intron
GATCCTTCCCATTAGTAACA+TGG 0.577737 6:-78998426 MsaT033189.1:CDS
ATCCTTCCCATTAGTAACAT+GGG 0.578409 6:-78998425 MsaT033189.1:CDS
ATTTATGTCAATAGGATCAG+TGG 0.578761 6:-78999888 MsaT033189.1:intron
GAGCTTGTCCTTTAGCAATG+TGG 0.579228 6:+78998219 None:intergenic
GAGTGGGATGCTTTGTCCAA+TGG 0.579467 6:-78998263 MsaT033189.1:CDS
GAACTTGGTATGGGAAGGAA+TGG 0.581764 6:-79000400 MsaT033189.1:CDS
GGAATGAATGGAACCAGGAG+TGG 0.582304 6:-78999808 MsaT033189.1:CDS
ATGGGATTTGGTATGGGAGA+CGG 0.583168 6:-79000340 MsaT033189.1:CDS
AAAATTGTCCAGGAATTGCT+GGG 0.583398 6:+79000566 None:intergenic
GCTGATCATCAGCAGCATCA+AGG 0.584879 6:+79001626 None:intergenic
GAAGCTCGCGCAGAGGATGT+CGG 0.587056 6:-78998495 MsaT033189.1:CDS
GGAATTAACCAATGGACGAT+AGG 0.591089 6:+78998870 None:intergenic
TGGTCGGCCGAAGAACTTGT+GGG 0.591669 6:+79000471 None:intergenic
GAATCGCCGAACTCAGATGA+AGG 0.591844 6:-79001105 MsaT033189.1:intron
GAAGCTCCAGCAATCATACA+TGG 0.592792 6:+78999853 None:intergenic
CCACCGCAATTGCTGCATGT+CGG 0.593169 6:+79000589 None:intergenic
AGGCAAATGCCGAGACTGGT+CGG 0.593975 6:+79000455 None:intergenic
TTGGACATCGTGATCAGTGG+TGG 0.594344 6:-78997735 MsaT033189.1:CDS
AATCCGACATGCAGCAATTG+CGG 0.597113 6:-79000592 MsaT033189.1:CDS
ACCACTGATCACGATGTCCA+AGG 0.597274 6:+78997737 None:intergenic
CTAGTGGAATGAATGGAACC+AGG 0.599081 6:-78999813 MsaT033189.1:CDS
GCTTCGAACTTGGTATGGGA+AGG 0.599779 6:-79000405 MsaT033189.1:CDS
AACAAATTCAGGAACTCGAA+AGG 0.602744 6:-79001561 MsaT033189.1:intron
GGCACAGATGAGAGGCGAGT+GGG 0.605317 6:-78998279 MsaT033189.1:CDS
ATCGTTGGAACTTCAAGTGA+CGG 0.606411 6:-78997639 MsaT033189.1:CDS
CTGTTATTAATCAACAGTGC+AGG 0.607247 6:-79000043 MsaT033189.1:CDS
GGAACTTCAAGTGACGGTAG+CGG 0.608019 6:-78997633 MsaT033189.1:CDS
ACTTAGCACGATGCTAGAAG+TGG 0.613778 6:+78998361 None:intergenic
TCCTCCAAAATACAGCCAGA+AGG 0.624254 6:+78999518 None:intergenic
CGAACCGGCACTAATGGAGA+AGG 0.624381 6:+78999665 None:intergenic
TCCAAGGATTGTGAATCGAT+TGG 0.625878 6:+78997753 None:intergenic
TGTGAATCCGACTGGAAACG+AGG 0.632294 6:-78997838 MsaT033189.1:CDS
AAATCAAACACCCTTTGCCG+AGG 0.632804 6:+78998311 None:intergenic
GGCGAGGGCGGAGACAATGG+TGG 0.633411 6:-78997603 MsaT033189.1:CDS
TCCACTAGATATGACATCCA+AGG 0.637956 6:+78999828 None:intergenic
TGGGATGCTTTGTCCAATGG+TGG 0.638372 6:-78998260 MsaT033189.1:CDS
GCATGCTGAAGCTCGCGCAG+AGG 0.639288 6:-78998502 MsaT033189.1:CDS
GCCATGAGAATATGATTCTG+AGG 0.640637 6:-79000669 MsaT033189.1:CDS
GATGTCGGATTACTTCTGGT+CGG 0.644453 6:-78998480 MsaT033189.1:CDS
CTGTGACATGAGGACTGCGA+GGG 0.644493 6:+78998792 None:intergenic
GCATTGCCGGAATTAACCAA+TGG 0.645151 6:+78998862 None:intergenic
AGGCACAGATGAGAGGCGAG+TGG 0.645582 6:-78998280 MsaT033189.1:CDS
AACAAATTTGCATACCTGTG+GGG 0.645738 6:+78998761 None:intergenic
CCGACATGCAGCAATTGCGG+TGG 0.651218 6:-79000589 MsaT033189.1:CDS
GTTGATGTTTCAGTCAACGT+CGG 0.651473 6:-78999587 MsaT033189.1:CDS
TTTGCAAAGCAGCATGTCGG+AGG 0.652279 6:-78999623 MsaT033189.1:CDS
ACTTCAAGTGACGGTAGCGG+CGG 0.655300 6:-78997630 MsaT033189.1:CDS
CAGGAGTGGATCTTTGCAAG+TGG 0.658333 6:-78999794 MsaT033189.1:intron
CATGCTGCTTTGCAAACCTG+AGG 0.658678 6:+78999630 None:intergenic
TCACAGTCTGATGACCCCAC+AGG 0.660401 6:-78998775 MsaT033189.1:intron
TTCTGAGGCAGGAAAATGAG+AGG 0.661509 6:-79000654 MsaT033189.1:CDS
GATGGGTTGCAACTCTCCAG+AGG 0.663990 6:-78998825 MsaT033189.1:CDS
GGAAGAATTGAAAAGCCAGA+AGG 0.664256 6:+78997687 None:intergenic
AGGTTTGCAAAGCAGCATGT+CGG 0.665230 6:-78999626 MsaT033189.1:CDS
GGTCATCAGACTGTGACATG+AGG 0.673200 6:+78998782 None:intergenic
GTGAATCCGACTGGAAACGA+GGG 0.674287 6:-78997837 MsaT033189.1:CDS
ACTGTGACATGAGGACTGCG+AGG 0.681781 6:+78998791 None:intergenic
TCTCCCATACCAAATCCCAT+CGG 0.689519 6:+79000343 None:intergenic
TAAGTGCTGCAACTTCTGTG+TGG 0.690299 6:-78998343 MsaT033189.1:CDS
TCCAAACTCTTAATCCAGAG+AGG 0.692635 6:+79000172 None:intergenic
TCAAACAAGTAAGCTCACAA+TGG 0.693857 6:-78997538 MsaT033189.1:CDS
TGTGGGTCAAGGTAGACACT+CGG 0.700917 6:+79000488 None:intergenic
AGGTCTTGTGGAAACGATGT+TGG 0.712153 6:-79000023 MsaT033189.1:CDS
CAATGGTGGACCAATGCAGG+AGG 0.741696 6:-78998246 MsaT033189.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 78997461 79002140 78997461 ID=MsaG033189
Chr6 mRNA 78997461 79002140 78997461 ID=MsaT033189.1;Parent=MsaG033189
Chr6 exon 78997461 78997862 78997461 ID=MsaT033189.1.exon10;Parent=MsaT033189.1
Chr6 CDS 78997461 78997862 78997461 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 78998184 78998548 78998184 ID=MsaT033189.1.exon9;Parent=MsaT033189.1
Chr6 CDS 78998184 78998548 78998184 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 78998776 78998941 78998776 ID=MsaT033189.1.exon8;Parent=MsaT033189.1
Chr6 CDS 78998776 78998941 78998776 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 78999496 78999708 78999496 ID=MsaT033189.1.exon7;Parent=MsaT033189.1
Chr6 CDS 78999496 78999708 78999496 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 78999795 78999896 78999795 ID=MsaT033189.1.exon6;Parent=MsaT033189.1
Chr6 CDS 78999795 78999896 78999795 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 79000018 79000704 79000018 ID=MsaT033189.1.exon5;Parent=MsaT033189.1
Chr6 CDS 79000018 79000704 79000018 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 79001106 79001223 79001106 ID=MsaT033189.1.exon4;Parent=MsaT033189.1
Chr6 CDS 79001106 79001223 79001106 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 79001562 79001678 79001562 ID=MsaT033189.1.exon3;Parent=MsaT033189.1
Chr6 CDS 79001562 79001678 79001562 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 79001714 79001784 79001714 ID=MsaT033189.1.exon2;Parent=MsaT033189.1
Chr6 CDS 79001714 79001784 79001714 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Chr6 exon 79002108 79002140 79002108 ID=MsaT033189.1.exon1;Parent=MsaT033189.1
Chr6 CDS 79002108 79002140 79002108 ID=cds.MsaT033189.1;Parent=MsaT033189.1
Gene Sequence

>MsaT033189.1

ATGAAAATGGTCTCTAACTTGTCTTTGATCACTAACAGGAAAATGGAAGGAAATGTTGGAATGGGACTTATTGGAGAAAACTTTGATTCTGGTCTGCTTGGAAGTGAGAATTTTGATGATTTTTCTGGTGATGACCTTGATGCTGCTGATGATCAGCCAAAGAGCAAGAAGAAGAAGAAATATCATAGGCACACTCCTCAACAAATTCAGGAACTCGAAAGTTTCTTCAAGGAGTGTCCACATCCTGATGAGAAGCAAAGAACTGATCTTAGCAAGAAGCTTGGTTTGGCGAACAAGCAAGTCAAGTTCTGGTTTCAGAATCGCCGAACTCAGATGAAGACGCAACTGGAACGCCATGAGAATATGATTCTGAGGCAGGAAAATGAGAGGCTCCGAGCTGAGAACAGTTTGTTGAAAGATGCTGTGTCAAATCCGACATGCAGCAATTGCGGTGGCCCAGCAATTCCTGGACAATTTTCACCGGAGGACCACCAGCTTAGGGTTGAAAATGCCAGACTGAAAGAGGAATTGGGCCGAGTGTCTACCTTGACCCACAAGTTCTTCGGCCGACCAGTCTCGGCATTTGCCTCCATAACTATGCCAAATCCAAATTCTGGCTTCGAACTTGGTATGGGAAGGAATGGTGTTGTTGGTCCAAGCAATTTCAACATGTCGCTGCCGATGGGATTTGGTATGGGAGACGGAGTTATGGGAGCTTCGGCGCTACAATCTGGATTGCAATCACCAATGGGAATGATCGGAAATAGTGCTCAACAGGAGAGAGCTATGCTTATTGATCTTGCAGGAGCTGCTATGGATGAACTAATCAATATGTCTCAGCCGGATTCTCCTCTCTGGATTAAGAGTTTGGATGGTGTGAATGAAGTGCTGAACTATGAAGAGTATGCAAAGATCAATTCTCCTTTTAACGATCCGAAACAAAATGGTTTTGTAACTGAAGCTACAAGACAAACTGGACTGTTATTAATCAACAGTGCAGGTCTTGTGGAAACGATGTTGGACGCGGATCAGTGGGCAGAGATGTTTCCATGTATGATTGCTGGAGCTTCAACCTTGGATGTCATATCTAGTGGAATGAATGGAACCAGGAGTGGATCTTTGCAAGTGATGCAAGCCGAGGTTCAAGTGCCTTCTCCATTAGTGCCGGTTCGACAATATAGCTTCCTCAGGTTTGCAAAGCAGCATGTCGGAGGCATTTGGGTTGTGGTTGATGTTTCAGTCAACGTCGGCCGTAATGCTGCTAATGGAAACACTTACATGAGCTGTAAGAAGCTTCCTTCTGGCTGTATTTTGGAGGATTTGCCCAATGGTTTTTGCAAGGTTACTTGGGTTGATCATTCCCAATATGATGAGAGTGTTGTCCACCAAGCCTATCGTCCATTGGTTAATTCCGGCAATGCATTTGGTGCTCATCGATGGGTTGCAACTCTCCAGAGGAAGTGTGATTCCCTCGCAGTCCTCATGTCACAGTCTGATGACCCCACAGCCATGACTCCACATGGTAAGAAAAGCATGCTGAAGCTCGCGCAGAGGATGTCGGATTACTTCTGGTCGGGAATCTGTCCTTCGAGTGCGTGCAAGTGGGAGATCCTTCCCATTAGTAACATGGGCGACAGTGACATGAAAATCATGTATCGGCAACATCCAGATGCCACTTCTAGCATCGTGCTAAGTGCTGCAACTTCTGTGTGGATTCCGTTGCCTCGGCAAAGGGTGTTTGATTTTCTCCGTGAGGCACAGATGAGAGGCGAGTGGGATGCTTTGTCCAATGGTGGACCAATGCAGGAGGTGGCCCACATTGCTAAAGGACAAGCTCTTGGAAATTCCATTTCTATTCTTAGTGTCAACAATGCTGTGAATCCGACTGGAAACGAGGGAAGCAGTCTATACCTGCAAGACTCATGGACCGATAGCTCTGGCTCAATGGTCGTGTACTCACCAATCGATTCACAATCCTTGGACATCGTGATCAGTGGTGGAGAGTCTTCATTGGTTCCTCTTTTACCTTCTGGCTTTTCAATTCTTCCGGACGGCCATTCAAACGTCAACAATATCGTTGGAACTTCAAGTGACGGTAGCGGCGGCAATGGCGAGGGCGGAGACAATGGTGGATGCTTGCTGACATTTGGATTACAAATGCTGCTGAACAACACTCAAACAAGTAAGCTCACAATGGAGTCAGTTGAGTCTGTGAATGCACAAATCTCAGTGACCATTCAGAGAATCAAAGATGCACTTGGAGTTGTTGCATGA
Protein sequence

>MsaT033189.1

MKMVSNLSLITNRKMEGNVGMGLIGENFDSGLLGSENFDDFSGDDLDAADDQPKSKKKKKYHRHTPQQIQELESFFKECPHPDEKQRTDLSKKLGLANKQVKFWFQNRRTQMKTQLERHENMILRQENERLRAENSLLKDAVSNPTCSNCGGPAIPGQFSPEDHQLRVENARLKEELGRVSTLTHKFFGRPVSAFASITMPNPNSGFELGMGRNGVVGPSNFNMSLPMGFGMGDGVMGASALQSGLQSPMGMIGNSAQQERAMLIDLAGAAMDELINMSQPDSPLWIKSLDGVNEVLNYEEYAKINSPFNDPKQNGFVTEATRQTGLLLINSAGLVETMLDADQWAEMFPCMIAGASTLDVISSGMNGTRSGSLQVMQAEVQVPSPLVPVRQYSFLRFAKQHVGGIWVVVDVSVNVGRNAANGNTYMSCKKLPSGCILEDLPNGFCKVTWVDHSQYDESVVHQAYRPLVNSGNAFGAHRWVATLQRKCDSLAVLMSQSDDPTAMTPHGKKSMLKLAQRMSDYFWSGICPSSACKWEILPISNMGDSDMKIMYRQHPDATSSIVLSAATSVWIPLPRQRVFDFLREAQMRGEWDALSNGGPMQEVAHIAKGQALGNSISILSVNNAVNPTGNEGSSLYLQDSWTDSSGSMVVYSPIDSQSLDIVISGGESSLVPLLPSGFSILPDGHSNVNNIVGTSSDGSGGNGEGGDNGGCLLTFGLQMLLNNTQTSKLTMESVESVNAQISVTIQRIKDALGVVA*