AlfalfaGEDB | MsaG033396

M. sativa ssp. caerulea / MsaG033396

KEGG: -
GO: GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033396	XP_024630580.1	95.263	190	9	0	1	190	2	191	2.21e-130	374

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033396	sp\|Q9FLI1\|BH036_ARATH	41.176	170	93	3	5	168	2	170	1.22e-32	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033396	tr\|A0A396HJA8\|A0A396HJA8_MEDTR	95.263	190	9	0	1	190	2	191	1.06e-130	374

TFs/TRs:

Gene ID	Type	Classification
MsaG033396	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000287	MsaG033396	0.801297	1.612570e-48	2.245050e-46
MsaG000308	MsaG033396	0.816141	1.117910e-51	2.224489e-49
MsaG000514	MsaG033396	0.856109	7.792842e-62	5.241245e-59
MsaG000759	MsaG033396	0.801376	1.553740e-48	2.167035e-46
MsaG001496	MsaG033396	0.820924	9.312064e-53	2.098767e-50
MsaG001660	MsaG033396	0.811560	1.130084e-50	2.004839e-48
MsaG002021	MsaG033396	0.802878	7.653212e-49	1.104624e-46
MsaG002420	MsaG033396	0.813964	3.382880e-51	6.371342e-49
MsaG004279	MsaG033396	0.845921	5.644816e-59	2.666413e-56
MsaG004513	MsaG033396	0.807147	9.889848e-50	1.576921e-47
MsaG004938	MsaG033396	0.811178	1.366598e-50	2.401823e-48
MsaG005346	MsaG033396	0.800175	2.724622e-48	3.698317e-46
MsaG006572	MsaG033396	0.832130	2.036953e-55	6.269557e-53
MsaG007006	MsaG033396	0.808765	4.495050e-50	7.451058e-48
MsaG009059	MsaG033396	0.819191	2.310220e-52	4.973992e-50
MsaG009179	MsaG033396	0.813927	3.446798e-51	6.485759e-49
MsaG009781	MsaG033396	0.833927	7.311603e-56	2.371779e-53
MsaG010213	MsaG033396	0.812690	6.422834e-51	1.171726e-48
MsaG010596	MsaG033396	0.829099	1.115107e-54	3.144996e-52
MsaG010768	MsaG033396	0.810683	1.746553e-50	3.032644e-48
MsaG011468	MsaG033396	0.838065	6.593413e-57	2.423045e-54
MsaG012023	MsaG033396	0.808205	5.909934e-50	9.665176e-48
MsaG012206	MsaG033396	0.800207	2.684358e-48	3.646261e-46
MsaG013338	MsaG033396	0.802661	8.481334e-49	1.218113e-46
MsaG014725	MsaG033396	0.805580	2.108704e-49	3.240229e-47
MsaG015294	MsaG033396	0.815728	1.380538e-51	2.718356e-49
MsaG017354	MsaG033396	0.844097	1.745295e-58	7.763497e-56
MsaG017464	MsaG033396	0.813191	4.994640e-51	9.226388e-49
MsaG017589	MsaG033396	0.834184	6.309733e-56	2.062455e-53
MsaG017737	MsaG033396	0.840207	1.847030e-57	7.257080e-55
MsaG018367	MsaG033396	0.820627	1.088583e-52	2.434161e-50
MsaG018891	MsaG033396	0.815198	1.809068e-51	3.514690e-49
MsaG019018	MsaG033396	0.818923	2.656973e-52	5.680281e-50
MsaG019093	MsaG033396	0.812809	6.051233e-51	1.107192e-48
MsaG019221	MsaG033396	0.801395	1.539828e-48	2.148578e-46
MsaG019296	MsaG033396	0.802520	9.063722e-49	1.297570e-46
MsaG020104	MsaG033396	0.825621	7.537002e-54	1.928589e-51
MsaG020402	MsaG033396	0.833341	1.022719e-55	3.260925e-53
MsaG020566	MsaG033396	0.823495	2.373106e-53	5.729890e-51
MsaG023474	MsaG033396	0.813333	4.650682e-51	8.621275e-49
MsaG023609	MsaG033396	0.872716	5.272883e-67	6.861131e-64
MsaG023941	MsaG033396	0.827278	3.049524e-54	8.172050e-52
MsaG025944	MsaG033396	0.807182	9.725679e-50	1.552025e-47
MsaG027136	MsaG033396	0.810281	2.131426e-50	3.664874e-48
MsaG027243	MsaG033396	0.805392	2.307860e-49	3.530825e-47
MsaG027303	MsaG033396	0.812288	7.857008e-51	1.419037e-48
MsaG027416	MsaG033396	0.852418	8.965391e-61	5.285132e-58
MsaG027494	MsaG033396	0.805052	2.716584e-49	4.123593e-47
MsaG028142	MsaG033396	0.807563	8.083260e-50	1.301658e-47
MsaG028263	MsaG033396	0.819590	1.876006e-52	4.081271e-50
MsaG028488	MsaG033396	0.819939	1.562910e-52	3.431790e-50
MsaG028862	MsaG033396	0.816125	1.126719e-51	2.241092e-49
MsaG028908	MsaG033396	0.845529	7.204026e-59	3.358833e-56
MsaG029132	MsaG033396	0.800479	2.364304e-48	3.231452e-46
MsaG029600	MsaG033396	0.845544	7.135763e-59	3.328818e-56
MsaG032202	MsaG033396	0.817828	4.690285e-52	9.746547e-50
MsaG033298	MsaG033396	0.828233	1.801740e-54	4.959386e-52
MsaG033396	MsaG033540	0.857641	2.771265e-62	1.971614e-59
MsaG033396	MsaG033519	0.858305	1.763216e-62	1.286008e-59
MsaG033396	MsaG033744	0.833404	9.860695e-56	3.150042e-53
MsaG033396	MsaG034220	0.856054	8.085416e-62	5.426989e-59
MsaG033396	MsaG034132	0.892574	3.112434e-74	1.051075e-70
MsaG033396	MsaG034548	0.815567	1.498769e-51	2.939122e-49
MsaG033396	MsaG034875	0.801956	1.182951e-48	1.671769e-46
MsaG033396	MsaG035039	0.809424	3.252503e-50	5.477721e-48
MsaG033396	MsaG035001	0.808522	5.062182e-50	8.342149e-48
MsaG033396	MsaG035190	0.830471	5.185900e-55	1.521109e-52
MsaG033396	MsaG035203	0.824799	1.176192e-53	2.942473e-51
MsaG033396	MsaG035236	0.816187	1.091889e-51	2.175190e-49
MsaG033396	MsaG039946	0.817519	5.502926e-52	1.134443e-49
MsaG033396	MsaG041916	0.808760	4.506316e-50	7.468885e-48
MsaG033396	MsaG042222	0.830098	6.390238e-55	1.854610e-52
MsaG033396	MsaG042327	0.831239	3.368682e-55	1.010157e-52
MsaG033396	MsaG042557	0.854219	2.744469e-61	1.724381e-58
MsaG033396	MsaG042627	0.802163	1.073047e-48	1.523607e-46
MsaG033396	MsaG043225	0.803711	5.154583e-49	7.584618e-47
MsaG033396	MsaG044225	0.803278	6.332043e-49	9.224252e-47
MsaG033396	MsaG044227	0.840080	1.992160e-57	7.796460e-55
MsaG033396	MsaG045285	0.828242	1.792862e-54	4.936194e-52
MsaG033396	MsaG045974	0.807923	6.782675e-50	1.101786e-47
MsaG033396	MsaG047135	0.849609	5.506148e-60	2.945085e-57
MsaG033396	MsaG002575	0.805226	2.499396e-49	3.809178e-47
MsaG033396	MsaG001460	0.825981	6.196012e-54	1.601560e-51
MsaG033396	MsaG010579	0.811412	1.216610e-50	2.150506e-48
MsaG033396	MsaG016780	0.833774	7.983079e-56	2.578023e-53
MsaG033396	MsaG013404	0.827757	2.343326e-54	6.364583e-52
MsaG033396	MsaG030579	0.815668	1.423521e-51	2.798749e-49
MsaG033396	MsaG032134	0.811808	9.987627e-51	1.782709e-48
MsaG033396	MsaG036784	0.808954	4.097200e-50	6.822553e-48
MsaG033396	MsaG034989	0.800193	2.702001e-48	3.669107e-46
MsaG033396	MsaG036671	0.819472	1.994892e-52	4.326738e-50
MsaG033396	MsaG040778	0.830439	5.279559e-55	1.547236e-52
MsaG033396	MsaG042135	0.823829	1.984404e-53	4.834963e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033396	MtrunA17_Chr6g0474681	95.263	190	9	0	1	190	2	191	2.03e-134	374
MsaG033396	MtrunA17_Chr1g0204351	40.782	179	96	3	1	175	71	243	2.95e-36	126
MsaG033396	MtrunA17_Chr1g0204361	37.356	174	102	3	3	172	17	187	3.98e-35	122
MsaG033396	MtrunA17_Chr6g0474671	40.936	171	96	2	2	172	68	233	1.13e-33	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG033396	AT5G51780.1	41.176	170	93	3	5	168	2	170	1.24e-33	118
MsaG033396	AT5G51780.2	41.420	169	94	2	5	168	25	193	3.60e-33	117
MsaG033396	AT5G51790.1	40.782	179	101	4	2	176	24	201	5.19e-32	115
MsaG033396	AT4G25410.1	37.572	173	103	3	9	176	47	219	5.97e-32	115
MsaG033396	AT5G51790.3	40.782	179	101	4	2	176	89	266	3.88e-31	114
MsaG033396	AT5G51790.4	42.581	155	84	4	2	152	24	177	1.70e-29	108
MsaG033396	AT4G25400.1	37.870	169	98	3	2	169	21	183	2.20e-29	107
MsaG033396	AT1G12540.1	42.541	181	82	7	3	168	73	246	3.39e-29	108
MsaG033396	AT5G51790.2	42.581	155	84	4	2	152	89	242	8.02e-29	108
MsaG033396	AT4G25400.2	37.059	170	98	4	2	169	21	183	1.08e-27	103
MsaG033396	AT1G62975.1	36.364	187	96	3	3	168	72	256	4.05e-26	100
MsaG033396	AT1G62975.2	36.517	178	90	3	3	159	72	247	6.38e-26	100

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAAGATATTGTCCATTTC+AGG	0.181886	6:+86813390	None:intergenic
TCTTCCTATAGAATATATTA+AGG	0.316735	6:-86816879	MsaT033396.1:intron
GAGTTCATTTGAACCTGAAA+TGG	0.385630	6:-86813403	MsaT033396.1:CDS
ATGTCTAACACTCTTGATAA+AGG	0.395077	6:+86815608	None:intergenic
CTATAGGAAGAAGCGATCTA+AGG	0.415824	6:+86816891	None:intergenic
TAATCAATTCCAGGCATTAT+CGG	0.420357	6:+86813576	None:intergenic
TCATGGAAGAAAACAAGAAA+TGG	0.438064	6:-86816970	MsaT033396.1:CDS
AGTTCTGAATAATCAATTCC+AGG	0.449519	6:+86813567	None:intergenic
ATCTCAAGTCCTCCAGGAAA+AGG	0.460588	6:+86815665	None:intergenic
TGTACGTCCAACATGATAGA+AGG	0.467567	6:-86815554	MsaT033396.1:CDS
ACTTGAGCATGATATTGCTT+CGG	0.469019	6:+86813530	None:intergenic
CTAACCTTAATATATTCTAT+AGG	0.474465	6:+86816875	None:intergenic
GAAGCAATTACAAGATAAGA+AGG	0.481546	6:-86815780	MsaT033396.1:CDS
GAATCTTCCTTCTATCATGT+TGG	0.498570	6:+86815547	None:intergenic
TTAGACATGCTGCTTAAAGA+AGG	0.501900	6:-86815593	MsaT033396.1:CDS
TTGAAGGAACCGATAATGCC+TGG	0.505304	6:-86813585	MsaT033396.1:intron
TAAGAAGGAAGAGTTAATGA+AGG	0.514489	6:-86815765	MsaT033396.1:CDS
CACACAATCTCAAGTCCTCC+AGG	0.527587	6:+86815659	None:intergenic
AATTTCTGATCATGTGAATG+AGG	0.530841	6:-86815837	MsaT033396.1:CDS
TCTAAGGTTGGTGCAGAGGT+TGG	0.539114	6:+86816907	None:intergenic
AAAGGTTGAACAATAACAAA+GGG	0.545126	6:+86815683	None:intergenic
GCGATCTAAGGTTGGTGCAG+AGG	0.557780	6:+86816903	None:intergenic
AAGAGACAGAAAGACAAAGA+AGG	0.564940	6:-86816940	MsaT033396.1:CDS
ACAAATGCCACTACTCAAGA+TGG	0.579156	6:-86815728	MsaT033396.1:CDS
AGGAAGAAGCGATCTAAGGT+TGG	0.595316	6:+86816895	None:intergenic
TAGACATGCTGCTTAAAGAA+GGG	0.597527	6:-86815592	MsaT033396.1:CDS
AAAAGGTTGAACAATAACAA+AGG	0.600268	6:+86815682	None:intergenic
CATATACACTATAAGATCTG+AGG	0.626322	6:-86815525	MsaT033396.1:intron
ACTAGAGCCATCTTGAGTAG+TGG	0.645479	6:+86815721	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	86813389	86816990	86813389	ID=MsaG033396
Chr6	mRNA	86813389	86816990	86813389	ID=MsaT033396.1;Parent=MsaG033396
Chr6	exon	86813389	86813435	86813389	ID=MsaT033396.1.exon4;Parent=MsaT033396.1
Chr6	CDS	86813389	86813435	86813389	ID=cds.MsaT033396.1;Parent=MsaT033396.1
Chr6	exon	86813532	86813601	86813532	ID=MsaT033396.1.exon3;Parent=MsaT033396.1
Chr6	CDS	86813532	86813601	86813532	ID=cds.MsaT033396.1;Parent=MsaT033396.1
Chr6	exon	86815526	86815870	86815526	ID=MsaT033396.1.exon2;Parent=MsaT033396.1
Chr6	CDS	86815526	86815870	86815526	ID=cds.MsaT033396.1;Parent=MsaT033396.1
Chr6	exon	86816880	86816990	86816880	ID=MsaT033396.1.exon1;Parent=MsaT033396.1
Chr6	CDS	86816880	86816990	86816880	ID=cds.MsaT033396.1;Parent=MsaT033396.1

Gene Sequence

>MsaT033396.1

ATGGAAGAAAACAAGAAATGGATGCATAAAGAGACAGAAAGACAAAGAAGGCAAGAGATGACCAACCTCTGCACCAACCTTAGATCGCTTCTTCCTATAGAATATATTAAGGGAAAACGCTCAATTTCTGATCATGTGAATGAGGCTACGAATTATATCAACCACCTACAAAATAAAGTGAAGCAATTACAAGATAAGAAGGAAGAGTTAATGAAGGTATCTAATTTGAGTACAAATGCCACTACTCAAGATGGCTCTAGTAGTTCAAATATACAACCCTTTGTTATTGTTCAACCTTTTCCTGGAGGACTTGAGATTGTGTGCAGTTACAGCTTCAACAAGTGTTTGTTTCCTTTATCAAGAGTGTTAGACATGCTGCTTAAAGAAGGGCTTAATTTGATAAGTTGTACGTCCAACATGATAGAAGGAAGATTCATATACACTATAAGATCTGAGGAACCGATAATGCCTGGAATTGATTATTCAGAACTGCAAAGAAAGCTCACCGAAGCAATATCATGCTCAAGTTTGCAAGAGAGTTCATTTGAACCTGAAATGGACAATATCTTGTGA

Protein sequence

>MsaT033396.1

MEENKKWMHKETERQRRQEMTNLCTNLRSLLPIEYIKGKRSISDHVNEATNYINHLQNKVKQLQDKKEELMKVSNLSTNATTQDGSSSSNIQPFVIVQPFPGGLEIVCSYSFNKCLFPLSRVLDMLLKEGLNLISCTSNMIEGRFIYTIRSEEPIMPGIDYSELQRKLTEAISCSSLQESSFEPEMDNIL*