AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033400 MtrunA17_Chr6g0474671 87.952 166 19 1 43 207 49 214 5.02e-102 294
MsaG033400 MtrunA17_Chr1g0204351 52.874 174 75 3 39 207 47 218 9.94e-55 174
MsaG033400 MtrunA17_Chr1g0204361 38.217 157 89 2 58 207 13 168 1.74e-32 115
MsaG033400 MtrunA17_Chr6g0474681 41.060 151 84 2 61 206 3 153 1.03e-30 111
MsaG033400 MtrunA17_Chr6g0472071 74.419 43 11 0 1 43 211 253 3.44e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033400 AT4G25400.1 42.500 160 71 4 58 207 19 167 8.81e-32 114
MsaG033400 AT4G25400.2 42.500 160 71 4 58 207 19 167 9.65e-32 114
MsaG033400 AT4G25410.1 39.333 150 81 2 68 207 47 196 1.20e-30 112
MsaG033400 AT5G51780.2 40.764 157 84 1 60 207 21 177 5.59e-30 110
MsaG033400 AT5G51780.1 40.909 154 82 1 63 207 1 154 8.96e-30 108
MsaG033400 AT5G51790.4 41.558 154 78 3 64 207 27 178 1.27e-28 106
MsaG033400 AT5G51790.1 41.558 154 78 3 64 207 27 178 1.45e-28 106
MsaG033400 AT5G51790.2 42.208 154 77 4 64 207 92 243 5.24e-28 106
MsaG033400 AT5G51790.3 41.558 154 78 3 64 207 92 243 5.38e-28 106
MsaG033400 AT1G62975.2 48.235 85 42 1 51 135 63 145 9.06e-20 84.7
MsaG033400 AT1G62975.1 48.235 85 42 1 51 135 63 145 9.95e-20 84.7
MsaG033400 AT1G12540.1 34.641 153 87 4 68 207 79 231 2.04e-18 81.3

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGAGTTACTAAAATTAT+TGG 0.157017 6:+86928585 MsaT033400.1:CDS
TTCTCAAAATTCCATTATTC+TGG 0.212014 6:-86928522 None:intergenic
TCATAAAGTAGTTCATTATT+TGG 0.217906 6:-86927425 None:intergenic
TTAAATGTTCACCAGAATAA+TGG 0.283582 6:+86928511 MsaT033400.1:CDS
ACTAAAATTATTGGCTAAAG+AGG 0.322448 6:+86928594 MsaT033400.1:CDS
GGAACAAAAGTTACTCCTAT+TGG 0.337704 6:+86922420 MsaT033400.1:CDS
GGATCGGAGAGATGCATAAT+AGG 0.379636 6:-86927537 None:intergenic
ATAGAGAGATTGAGAAGAAA+AGG 0.391251 6:+86927498 MsaT033400.1:CDS
CAAAATAATATCAATAAACT+TGG 0.435192 6:+86928412 MsaT033400.1:CDS
AAATAATGAACTACTTTATG+AGG 0.483202 6:+86927427 MsaT033400.1:CDS
TACATGACTTGTACTCCAAT+AGG 0.494899 6:-86922435 None:intergenic
AAAACTCGCCAATTCTAAGC+TGG 0.500520 6:+86928456 MsaT033400.1:CDS
GAAGAAAAGGAGGCAAGAAA+TGG 0.503359 6:+86927511 MsaT033400.1:CDS
ATTTACAAGTGGCTTAAAAG+AGG 0.506030 6:+86928549 MsaT033400.1:CDS
ATACCTCACACATGACACTT+TGG 0.523655 6:-86928666 None:intergenic
GCTTCCTCTTGAGTTCATCA+AGG 0.524427 6:+86927562 MsaT033400.1:CDS
TTGAGAATTGAATTTACAAG+TGG 0.546189 6:+86928538 MsaT033400.1:CDS
CTAAAATTATTGGCTAAAGA+GGG 0.551865 6:+86928595 MsaT033400.1:CDS
CAAGTGGCTTAAAAGAGGAA+AGG 0.561553 6:+86928554 MsaT033400.1:CDS
TGCGTTACAACAGAAATCAA+TGG 0.564243 6:+86928634 MsaT033400.1:CDS
ATAATTCTAATGAACAAATG+AGG 0.576628 6:+86927465 MsaT033400.1:CDS
CATTTAAATTGCATGATGCA+TGG 0.579018 6:-86928495 None:intergenic
AATATCTGATCATACGAATG+AGG 0.579968 6:+86928366 MsaT033400.1:CDS
CTAACCTTGATGAACTCAAG+AGG 0.593616 6:-86927566 None:intergenic
GATGAACTCAAGAGGAAGCA+AGG 0.597305 6:-86927558 None:intergenic
GAGAGATTGAGAAGAAAAGG+AGG 0.599248 6:+86927501 MsaT033400.1:CDS
ACTCAAGAGGAAGCAAGGAT+CGG 0.611742 6:-86927553 None:intergenic
GAACTTGATCAAAAGCAATG+AGG 0.650045 6:+86922495 MsaT033400.1:CDS
GCTCCAAAGTGTCATGTGTG+AGG 0.704417 6:+86928663 MsaT033400.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 86922418 86928690 86922418 ID=MsaG033400
Chr6 mRNA 86922418 86928690 86922418 ID=MsaT033400.1;Parent=MsaG033400
Chr6 exon 86922418 86922546 86922418 ID=MsaT033400.1.exon1;Parent=MsaT033400.1
Chr6 CDS 86922418 86922546 86922418 ID=cds.MsaT033400.1;Parent=MsaT033400.1
Chr6 exon 86927425 86927583 86927425 ID=MsaT033400.1.exon2;Parent=MsaT033400.1
Chr6 CDS 86927425 86927583 86927425 ID=cds.MsaT033400.1;Parent=MsaT033400.1
Chr6 exon 86928355 86928690 86928355 ID=MsaT033400.1.exon3;Parent=MsaT033400.1
Chr6 CDS 86928355 86928690 86928355 ID=cds.MsaT033400.1;Parent=MsaT033400.1
Gene Sequence

>MsaT033400.1

ATGGAACAAAAGTTACTCCTATTGGAGTACAAGTCATGTACTGAAGCTAAGCTAGAAGAAGCTAGAGCAAGAGAAGAGAACTTGATCAAAAGCAATGAGGAATTCAAGAATGATATGAAGCTACAACAACCAAATAATGAACTACTTTATGAGGCTGCTAAGAATCATGATAATTCTAATGAACAAATGAGGAAAATGATTCATAGAGAGATTGAGAAGAAAAGGAGGCAAGAAATGGCAACCTATTATGCATCTCTCCGATCCTTGCTTCCTCTTGAGTTCATCAAGGGAAAGCGATCAATATCTGATCATACGAATGAGGCAGTGAATTACATAAAACACATGCAAAATAATATCAATAAACTTGGTGCCAAGAGAGATGAACTCAAAAAACTCGCCAATTCTAAGCTGGAAAATAATGAGAGCAACCATGCATCATGCAATTTAAATGTTCACCAGAATAATGGAATTTTGAGAATTGAATTTACAAGTGGCTTAAAAGAGGAAAGGCTCAAACTTTCAGAGTTACTAAAATTATTGGCTAAAGAGGGACTTGAAGTTGATAGTTGCGTTACAACAGAAATCAATGGTAGATTGCTCCAAAGTGTCATGTGTGAGGTATAA

Protein sequence

>MsaT033400.1

MEQKLLLLEYKSCTEAKLEEARAREENLIKSNEEFKNDMKLQQPNNELLYEAAKNHDNSNEQMRKMIHREIEKKRRQEMATYYASLRSLLPLEFIKGKRSISDHTNEAVNYIKHMQNNINKLGAKRDELKKLANSKLENNESNHASCNLNVHQNNGILRIEFTSGLKEERLKLSELLKLLAKEGLEVDSCVTTEINGRLLQSVMCEV*