AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033603

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033603 MtrunA17_Chr2g0308841 67.407 135 44 0 168 302 92 226 9.37e-63 204
MsaG033603 MtrunA17_Chr1g0203051 34.302 172 107 2 1 172 9 174 1.52e-26 113
MsaG033603 MtrunA17_Chr1g0203051 37.179 156 87 3 145 290 467 621 1.60e-24 107
MsaG033603 MtrunA17_Chr1g0171741 36.126 191 95 4 8 172 38 227 1.04e-23 100
MsaG033603 MtrunA17_Chr8g0389811 36.441 118 75 0 55 172 62 179 1.18e-23 98.6
MsaG033603 MtrunA17_Chr2g0287651 29.730 259 162 5 172 425 501 744 2.03e-22 100
MsaG033603 MtrunA17_Chr2g0287651 34.021 97 64 0 76 172 74 170 1.91e-14 75.9
MsaG033603 MtrunA17_Chr2g0331371 36.029 136 78 1 64 199 42 168 2.34e-21 92.0
MsaG033603 MtrunA17_Chr6g0473421 38.760 129 75 2 27 152 3 130 4.41e-21 89.7
MsaG033603 MtrunA17_Chr1g0172931 37.931 116 70 1 44 159 45 158 1.64e-19 85.5
MsaG033603 MtrunA17_Chr3g0118611 30.435 161 108 2 175 335 374 530 1.83e-15 79.3
MsaG033603 MtrunA17_Chr5g0448511 31.008 129 88 1 175 303 270 397 1.07e-13 73.2
MsaG033603 MtrunA17_Chr5g0440451 28.022 182 125 4 175 355 477 653 1.33e-13 73.6
MsaG033603 MtrunA17_Chr5g0448761 28.205 156 109 2 175 328 498 652 1.78e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033603 AT2G27110.2 31.298 131 89 1 176 306 453 582 3.81e-15 78.6
MsaG033603 AT2G27110.1 31.298 131 89 1 176 306 453 582 3.81e-15 78.6
MsaG033603 AT2G27110.3 31.298 131 89 1 176 306 308 437 4.61e-15 78.2
MsaG033603 AT4G38170.1 31.818 132 89 1 172 303 269 399 1.48e-12 70.1

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAAAGCATTGCTTATC+AGG 0.267259 6:-95092147 MsaT033603.1:CDS
CAATCTGCAGCCAGATATTC+TGG 0.285407 6:-95091839 MsaT033603.1:CDS
CGATGAGCTTCCCAATTGTT+TGG 0.309675 6:-95091939 MsaT033603.1:CDS
CGACTAAATTTAAATCTTCC+TGG 0.318399 6:-95091518 MsaT033603.1:CDS
TGTAGTAATTGTGGTGTAAT+AGG 0.325728 6:-95091563 MsaT033603.1:CDS
ATATTTACCGTAGGCAAAAC+TGG 0.339586 6:+95091680 None:intergenic
ATTCAAGTGGTTCTTTGTAC+TGG 0.339799 6:-95091868 MsaT033603.1:CDS
CAGCAACTTCTTTGCACAAC+TGG 0.354915 6:+95091810 None:intergenic
GCAATTATGTTTAACGATAT+AGG 0.362555 6:-95093617 MsaT033603.1:CDS
TCTGATTTCCAATCTAACTA+TGG 0.363449 6:-95092262 MsaT033603.1:CDS
CGTTGCGTGGCTCACGCTTT+CGG 0.364192 6:+95093366 None:intergenic
CTGGACTATACCAGAATATC+TGG 0.365414 6:+95091829 None:intergenic
TGTTGGTTTGCTAGAATGTC+CGG 0.368843 6:-95093503 MsaT033603.1:CDS
CAGGTTGACCATAGTTAGAT+TGG 0.371643 6:+95092254 None:intergenic
TACGTAAGGCTCAAATGATT+AGG 0.371897 6:-95093472 MsaT033603.1:CDS
ATAAAACAGCATAATGTTAC+AGG 0.374647 6:-95093795 None:intergenic
TCTAGGCAAAACCAAACAAT+TGG 0.377581 6:+95091928 None:intergenic
GAAGATGGATTAGATGGTTC+AGG 0.385947 6:-95093647 MsaT033603.1:CDS
CCTGAGGTTGTTAGAACAAA+AGG 0.400378 6:-95091638 MsaT033603.1:CDS
ACATAAAATGTTCTTGTTTG+AGG 0.406668 6:-95092021 MsaT033603.1:CDS
TGAAGGATTTAGAGAGGATA+GGG 0.408657 6:-95093405 MsaT033603.1:CDS
GATAAACGATTGTCAAGAAA+TGG 0.410498 6:-95092125 MsaT033603.1:CDS
TCTGTTGAAGATGGATTAGA+TGG 0.422289 6:-95093653 MsaT033603.1:CDS
ACGTAAGGCTCAAATGATTA+GGG 0.435617 6:-95093471 MsaT033603.1:CDS
TTCAAATTCGTCTTCACCAT+TGG 0.445525 6:+95091478 None:intergenic
GTCAACCGGTTCTTCGCAAA+AGG 0.448183 6:+95092042 None:intergenic
TAGCAAACCAACAATAAAAC+AGG 0.448653 6:+95093513 None:intergenic
GGCTCAAATGATTAGGGATC+GGG 0.451003 6:-95093465 MsaT033603.1:CDS
TGCGTGCGTTTGCGCCTGCC+TGG 0.457056 6:+95091590 None:intergenic
TTTGCAAATTTCGACCATCT+AGG 0.458519 6:+95091911 None:intergenic
TTCTAACAACCTCAGGATCA+AGG 0.458940 6:+95091645 None:intergenic
AAACTCACAAGTATCTTTGC+CGG 0.460720 6:-95093699 MsaT033603.1:CDS
GAGGCAAATTCTGTTGAAGA+TGG 0.463355 6:-95093662 MsaT033603.1:CDS
AATTCGTCTTCACCATTGGC+AGG 0.467391 6:+95091482 None:intergenic
CCACATCTTGTTTCGTTGCG+TGG 0.468890 6:+95093353 None:intergenic
CGAAACAAGATGTGGATGTA+AGG 0.474068 6:-95093345 MsaT033603.1:CDS
TATCGTTAAACATAATTGCT+TGG 0.479970 6:+95093621 None:intergenic
AAAATGTTCTTGTTTGAGGA+TGG 0.482756 6:-95092017 MsaT033603.1:CDS
TTCAAGTGGTTCTTTGTACT+GGG 0.484456 6:-95091867 MsaT033603.1:CDS
GATTGTCAAGAAATGGCTAC+AGG 0.494454 6:-95092118 MsaT033603.1:CDS
TGTTTGAGGATGGAGTCATT+CGG 0.499022 6:-95092007 MsaT033603.1:CDS
CAGTTGTGCAAAGAAGTTGC+TGG 0.503687 6:-95091809 MsaT033603.1:CDS
GTCCGGGTTCTCTGTACGTA+AGG 0.510316 6:-95093486 MsaT033603.1:CDS
GCCTCTTCTTCAGATTCGAC+CGG 0.514599 6:+95093680 None:intergenic
AGGATGTGGTTCAATTGCTA+CGG 0.521795 6:-95091618 MsaT033603.1:CDS
TTGCTACGGGTACTCCAGGC+AGG 0.522386 6:-95091604 MsaT033603.1:CDS
GTGAAGGATTTAGAGAGGAT+AGG 0.527849 6:-95093406 MsaT033603.1:CDS
CGTAGACTGGTTTGTAACAC+AGG 0.531887 6:+95092235 None:intergenic
GTTGTTAGAACAAAAGGATG+TGG 0.535006 6:-95091632 MsaT033603.1:CDS
TCAATTGCTACGGGTACTCC+AGG 0.535102 6:-95091608 MsaT033603.1:CDS
GAAGGATTTAGAGAGGATAG+GGG 0.551534 6:-95093404 MsaT033603.1:CDS
CTAGGCAAAACCAAACAATT+GGG 0.553863 6:+95091929 None:intergenic
AGGCTCAAATGATTAGGGAT+CGG 0.556672 6:-95093466 MsaT033603.1:CDS
TAGACATTGTTACAAAGCGT+TGG 0.557208 6:-95093304 MsaT033603.1:intron
ACCGTCTTGATGATAGTGAA+TGG 0.559451 6:-95092072 MsaT033603.1:CDS
GTTGGTTTGCTAGAATGTCC+GGG 0.563741 6:-95093502 MsaT033603.1:CDS
TTTCTCGATAGAACGTAGAC+TGG 0.573149 6:+95092222 None:intergenic
GGAAGATTTAAATTTAGTCG+TGG 0.577931 6:+95091521 None:intergenic
GGATGTGGTTCAATTGCTAC+GGG 0.579078 6:-95091617 MsaT033603.1:CDS
GCCGGTCGAATCTGAAGAAG+AGG 0.579413 6:-95093681 MsaT033603.1:CDS
TCGTCTTCCATATTTACCGT+AGG 0.581045 6:+95091671 None:intergenic
TTCATGTGAAGGATTTAGAG+AGG 0.585894 6:-95093411 MsaT033603.1:CDS
AAGGATTTAGAGAGGATAGG+GGG 0.589938 6:-95093403 MsaT033603.1:CDS
GTGGACAACATCGTCTATTG+TGG 0.591454 6:+95091540 None:intergenic
AGCCTTACGTACAGAGAACC+CGG 0.602665 6:+95093484 None:intergenic
AGCTGCAAAGTTGTTTACGA+AGG 0.607404 6:-95092197 MsaT033603.1:CDS
ACGCAAACTTGTAGTAATTG+TGG 0.610538 6:-95091572 MsaT033603.1:CDS
ATTCGTGAGAAGTATTCAAG+TGG 0.612122 6:-95091881 MsaT033603.1:CDS
TGATATAATGAAGTTTGACA+TGG 0.626135 6:-95093594 MsaT033603.1:CDS
AGACGAACTCCTTGATCCTG+AGG 0.627835 6:-95091654 MsaT033603.1:CDS
GATGGTAGCAACACGTCAAG+CGG 0.631845 6:-95093755 MsaT033603.1:CDS
ATTGTCAAGAAATGGCTACA+GGG 0.634645 6:-95092117 MsaT033603.1:CDS
CATACACTTAATCCTGCCAA+TGG 0.635369 6:-95091494 MsaT033603.1:CDS
TTTGCATTGATGATGTTGGA+AGG 0.635495 6:-95093565 MsaT033603.1:CDS
AAACCAACAATAAAACAGGT+AGG 0.641669 6:+95093517 None:intergenic
CTACTATAGTCAGACTCAGA+CGG 0.647730 6:-95091435 MsaT033603.1:intron
GCCATTCACTATCATCAAGA+CGG 0.655881 6:+95092071 None:intergenic
CCACGCAACGAAACAAGATG+TGG 0.660406 6:-95093353 MsaT033603.1:CDS
GGTAGCAACACGTCAAGCGG+TGG 0.661636 6:-95093752 MsaT033603.1:CDS
ACTGCTATTATATGATCACA+TGG 0.668256 6:+95091980 None:intergenic
GATGGATTAGATGGTTCAGG+TGG 0.668303 6:-95093644 MsaT033603.1:CDS
GCAGGATTAAGTGTATGACC+AGG 0.677736 6:+95091500 None:intergenic
GACACGACAGAAGAAAGTGA+TGG 0.695407 6:-95093773 MsaT033603.1:CDS
CAAATGATTAGGGATCGGGA+AGG 0.746751 6:-95093461 MsaT033603.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 95091300 95093804 95091300 ID=MsaG033603
Chr6 mRNA 95091300 95093804 95091300 ID=MsaT033603.1;Parent=MsaG033603
Chr6 exon 95091300 95091320 95091300 ID=MsaT033603.1.exon3;Parent=MsaT033603.1
Chr6 CDS 95091300 95091320 95091300 ID=cds.MsaT033603.1;Parent=MsaT033603.1
Chr6 exon 95091436 95092318 95091436 ID=MsaT033603.1.exon2;Parent=MsaT033603.1
Chr6 CDS 95091436 95092318 95091436 ID=cds.MsaT033603.1;Parent=MsaT033603.1
Chr6 exon 95093305 95093804 95093305 ID=MsaT033603.1.exon1;Parent=MsaT033603.1
Chr6 CDS 95093305 95093804 95093305 ID=cds.MsaT033603.1;Parent=MsaT033603.1
Gene Sequence

>MsaT033603.1

ATGTTACAGGACACGACAGAAGAAAGTGATGGTAGCAACACGTCAAGCGGTGGTTGTAGTACTACAGTCGAAACTGAAAACTCAAACTCACAAGTATCTTTGCCGGTCGAATCTGAAGAAGAGGCAAATTCTGTTGAAGATGGATTAGATGGTTCAGGTGGCCAAGCAATTATGTTTAACGATATAGGTGATATAATGAAGTTTGACATGGAAAAGTTTTGCATTGATGATGTTGGAAGGTATGATTTTGCTAGTTTGGATGTTGCCTACCTGTTTTATTGTTGGTTTGCTAGAATGTCCGGGTTCTCTGTACGTAAGGCTCAAATGATTAGGGATCGGGAAGGTGTTGTTTTGCAGCAAACGTTTTTATGTTCATGTGAAGGATTTAGAGAGGATAGGGGGTTAACAAGGGAGAACCGAAAGCGTGAGCCACGCAACGAAACAAGATGTGGATGTAAGGCAAAATTTAGAGTTCATGTAGACATTGTTACAAAGCGTTGGTGTCTAACTTATTTTCGTTTCAGGGAAATTGAATCTGATTTCCAATCTAACTATGGTCAACCTGTGTTACAAACCAGTCTACGTTCTATCGAGAAATCAGCTGCAAAGTTGTTTACGAAGGAGATCTTTTTCTTGTTTAGGTCGGTACTAAAGAAAGCATTGCTTATCAGGATAAACGATTGTCAAGAAATGGCTACAGGGTGCATCTTTAAAGTTTCAAAGTACCGTCTTGATGATAGTGAATGGCTTGTTTCCTTTTGCGAAGAACCGGTTGACATAAAATGTTCTTGTTTGAGGATGGAGTCATTCGGATTGCCATGTGATCATATAATAGCAGTGTTGTTGTATCTTGATTTCGATGAGCTTCCCAATTGTTTGGTTTTGCCTAGATGGTCGAAATTTGCAAAAGATTTAATTCGTGAGAAGTATTCAAGTGGTTCTTTGTACTGGGATTCACAATCTGCAGCCAGATATTCTGGTATAGTCCAGTTGTGCAAAGAAGTTGCTGGATTAGTTTATGAAGATCTTGATGAGTATAATGATTTTGTTGAAAGCTTGAGTGGGGAAATCCATAGATTAAAATTAAAGAAAAATGTTGAATCTTCTGAATTATCTCCAGTTTTGCCTACGGTAAATATGGAAGACGAACTCCTTGATCCTGAGGTTGTTAGAACAAAAGGATGTGGTTCAATTGCTACGGGTACTCCAGGCAGGCGCAAACGCACGCAAACTTGTAGTAATTGTGGTGTAATAGGCCACAATAGACGATGTTGTCCACGACTAAATTTAAATCTTCCTGGTCATACACTTAATCCTGCCAATGGTGAAGACGAATTTGAAGAAGACGATGACAACTACTACTACTATAGTCAGACTCAGACGGATATTGGCACACAAGATTGA
Protein sequence

>MsaT033603.1

MLQDTTEESDGSNTSSGGCSTTVETENSNSQVSLPVESEEEANSVEDGLDGSGGQAIMFNDIGDIMKFDMEKFCIDDVGRYDFASLDVAYLFYCWFARMSGFSVRKAQMIRDREGVVLQQTFLCSCEGFREDRGLTRENRKREPRNETRCGCKAKFRVHVDIVTKRWCLTYFRFREIESDFQSNYGQPVLQTSLRSIEKSAAKLFTKEIFFLFRSVLKKALLIRINDCQEMATGCIFKVSKYRLDDSEWLVSFCEEPVDIKCSCLRMESFGLPCDHIIAVLLYLDFDELPNCLVLPRWSKFAKDLIREKYSSGSLYWDSQSAARYSGIVQLCKEVAGLVYEDLDEYNDFVESLSGEIHRLKLKKNVESSELSPVLPTVNMEDELLDPEVVRTKGCGSIATGTPGRRKRTQTCSNCGVIGHNRRCCPRLNLNLPGHTLNPANGEDEFEEDDDNYYYYSQTQTDIGTQD*