AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033633


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033633 MtrunA17_Chr6g0477001 96.850 254 8 0 25 278 86 339 0.0 519
MsaG033633 MtrunA17_Chr2g0305371 56.707 164 69 1 29 192 313 474 2.66e-61 201
MsaG033633 MtrunA17_Chr3g0125251 44.186 172 93 3 22 190 1294 1465 3.27e-40 148
MsaG033633 MtrunA17_Chr1g0201311 45.882 170 90 2 25 192 57 226 3.81e-39 142
MsaG033633 MtrunA17_Chr1g0201621 45.294 170 91 2 25 192 57 226 1.59e-38 140
MsaG033633 MtrunA17_Chr4g0075321 34.444 180 102 3 26 189 884 1063 3.92e-25 104
MsaG033633 MtrunA17_Chr8g0373821 34.682 173 100 2 26 185 361 533 6.70e-25 103
MsaG033633 MtrunA17_Chr5g0403241 35.000 180 101 3 26 189 1327 1506 1.02e-24 103
MsaG033633 MtrunA17_Chr7g0221611 38.129 139 81 2 51 186 905 1041 1.68e-20 91.3
MsaG033633 MtrunA17_Chr2g0297191 35.294 136 88 0 51 186 1032 1167 2.92e-20 90.5
MsaG033633 MtrunA17_Chr1g0149611 34.483 145 89 2 51 189 363 507 1.16e-19 88.6
MsaG033633 MtrunA17_Chr7g0275611 38.776 147 75 4 55 187 816 961 2.02e-19 88.2
MsaG033633 MtrunA17_Chr4g0003511 30.000 200 106 6 24 189 852 1051 1.03e-18 85.9
MsaG033633 MtrunA17_Chr5g0399801 29.949 197 104 6 23 185 888 1084 1.12e-18 85.9
MsaG033633 MtrunA17_Chr7g0255181 29.189 185 109 4 24 186 517 701 3.00e-18 84.3
MsaG033633 MtrunA17_Chr1g0149581 32.414 145 92 2 51 189 1521 1665 3.33e-18 84.7
MsaG033633 MtrunA17_Chr3g0126701 37.692 130 76 3 62 187 932 1060 4.32e-18 84.3
MsaG033633 MtrunA17_Chr4g0003501 29.703 202 102 6 24 189 757 954 1.03e-17 83.2
MsaG033633 MtrunA17_Chr3g0080761 27.778 198 100 4 24 187 459 647 2.96e-16 78.6
MsaG033633 MtrunA17_Chr1g0147121 36.923 130 77 3 62 187 903 1031 3.23e-16 78.6
MsaG033633 MtrunA17_Chr8g0347071 35.385 130 79 3 62 187 818 946 6.14e-16 77.8
MsaG033633 MtrunA17_Chr8g0354821 27.317 205 111 5 24 190 484 688 1.13e-14 73.9
MsaG033633 MtrunA17_Chr5g0401921 33.077 130 85 2 40 169 703 830 1.81e-14 73.6
MsaG033633 MtrunA17_Chr1g0194631 32.847 137 87 3 40 176 599 730 3.21e-14 72.4
MsaG033633 MtrunA17_Chr7g0235081 30.579 121 84 0 51 171 194 314 2.65e-12 66.2
MsaG033633 MtrunA17_Chr6g0460921 26.882 186 110 7 30 195 243 422 7.18e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033633 AT2G44150.1 63.475 282 68 6 30 276 82 363 6.00e-118 343
MsaG033633 AT3G59960.1 58.103 253 74 5 30 250 77 329 1.33e-90 272
MsaG033633 AT3G59960.3 71.823 181 38 1 30 197 77 257 1.69e-87 263
MsaG033633 AT3G59960.2 71.823 181 38 1 30 197 77 257 1.69e-87 263
MsaG033633 AT3G59960.4 71.823 181 38 1 30 197 77 257 1.69e-87 263
MsaG033633 AT4G30860.1 54.167 168 75 1 29 196 306 471 1.88e-60 199
MsaG033633 AT1G77300.2 42.286 175 98 3 22 193 996 1170 2.79e-37 140
MsaG033633 AT1G77300.1 42.286 175 98 3 22 193 996 1170 2.89e-37 140
MsaG033633 AT1G76710.4 44.910 167 90 2 28 192 65 231 8.93e-37 136
MsaG033633 AT1G76710.2 44.910 167 90 2 28 192 65 231 9.14e-37 136
MsaG033633 AT1G76710.1 44.910 167 90 2 28 192 65 231 9.14e-37 136
MsaG033633 AT1G76710.3 44.910 167 90 2 28 192 65 231 1.13e-36 136
MsaG033633 AT2G23740.1 35.593 177 93 4 30 186 1196 1371 3.97e-23 99.4
MsaG033633 AT2G23740.3 35.593 177 93 4 30 186 1203 1378 4.01e-23 99.4
MsaG033633 AT2G23740.6 35.593 177 93 4 30 186 1203 1378 4.01e-23 99.4
MsaG033633 AT2G23740.2 35.593 177 93 4 30 186 1203 1378 4.01e-23 99.4
MsaG033633 AT5G42400.7 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.2 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.8 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.5 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.4 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.1 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.3 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT5G42400.6 39.683 126 76 0 64 189 1262 1387 1.12e-20 92.4
MsaG033633 AT2G35160.5 33.871 186 103 5 16 189 580 757 1.16e-20 92.0
MsaG033633 AT2G35160.2 33.871 186 103 5 16 189 616 793 1.29e-20 92.0
MsaG033633 AT2G35160.3 33.871 186 103 5 16 189 616 793 1.29e-20 92.0
MsaG033633 AT2G35160.1 33.871 186 103 5 16 189 616 793 1.29e-20 92.0
MsaG033633 AT2G35160.4 33.871 186 103 5 16 189 616 793 1.29e-20 92.0
MsaG033633 AT1G05830.2 36.691 139 83 2 51 186 919 1055 1.54e-18 85.9
MsaG033633 AT1G05830.4 36.691 139 83 2 51 186 919 1055 1.54e-18 85.9
MsaG033633 AT1G05830.1 36.691 139 83 2 51 186 919 1055 1.54e-18 85.9
MsaG033633 AT1G05830.3 36.691 139 83 2 51 186 919 1055 1.54e-18 85.9
MsaG033633 AT2G22740.2 31.944 216 105 8 13 189 577 789 2.04e-18 85.5
MsaG033633 AT2G22740.1 31.944 216 105 8 13 189 577 789 2.04e-18 85.5
MsaG033633 AT2G31650.1 36.691 139 83 2 51 186 898 1034 2.92e-18 85.1
MsaG033633 AT4G27910.1 38.462 130 75 3 62 187 896 1024 1.52e-17 82.8
MsaG033633 AT5G53430.1 36.923 130 77 3 62 187 912 1040 7.40e-16 77.8
MsaG033633 AT3G61740.1 38.168 131 75 4 62 187 886 1015 7.43e-16 77.8
MsaG033633 AT1G02580.1 34.177 158 94 3 26 175 511 666 8.35e-16 77.8
MsaG033633 AT3G61740.2 38.168 131 75 4 62 187 850 979 1.04e-15 77.4
MsaG033633 AT1G17770.1 28.774 212 112 6 12 186 480 689 1.38e-15 77.0
MsaG033633 AT3G03750.2 29.189 185 106 6 30 193 170 350 1.83e-14 72.8
MsaG033633 AT4G02020.1 32.847 137 87 3 40 176 696 827 2.29e-14 73.6
MsaG033633 AT5G04940.2 27.586 203 110 5 24 190 469 670 2.81e-14 73.2
MsaG033633 AT5G04940.1 27.586 203 110 5 24 190 469 670 2.81e-14 73.2
MsaG033633 AT5G13960.2 28.141 199 104 5 30 189 291 489 6.45e-14 72.0
MsaG033633 AT5G13960.1 28.141 199 104 5 30 189 425 623 1.21e-13 71.2
MsaG033633 AT2G23380.1 36.111 108 67 2 62 169 763 868 7.59e-12 65.9
MsaG033633 AT2G23380.2 36.111 108 67 2 62 169 536 641 8.60e-12 65.9
MsaG033633 AT2G24740.1 27.551 196 97 6 35 190 565 755 5.41e-11 63.2
MsaG033633 AT2G23740.4 35.398 113 60 2 30 130 1203 1314 7.34e-11 63.2
MsaG033633 AT2G23740.5 35.398 113 60 2 30 130 1203 1314 7.34e-11 63.2

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGATATGCCACTAATTT+AGG 0.249497 6:-95850699 None:intergenic
GCAGATTTGGGATTATTAAA+TGG 0.252262 6:+95855154 MsaT033633.1:intron
CATAGCAGGTTTGTTCAATT+TGG 0.254145 6:+95850609 MsaT033633.1:intron
CATTTCTGCATCTCAGTATT+CGG 0.269242 6:-95849975 None:intergenic
GCCTAAATTAGTGGCATATC+AGG 0.297012 6:+95850698 MsaT033633.1:CDS
CTTCTGTCTAGCTGTTCTTC+AGG 0.341155 6:+95849286 MsaT033633.1:CDS
CAGTATTCGGGCAGCAACTA+TGG 0.356191 6:-95849962 None:intergenic
GTGATAGATGCGACAAATAA+AGG 0.361111 6:+95849918 MsaT033633.1:CDS
AAACAAGCTTACCAGTTTGC+AGG 0.361797 6:-95854806 None:intergenic
TCCATCTTTAGGATAATAGA+TGG 0.401604 6:+95850132 MsaT033633.1:intron
TGTTCAATGTAGGCGTAAGC+TGG 0.427738 6:+95850659 MsaT033633.1:CDS
ATGTGAAATCAATCGAGATA+TGG 0.429587 6:+95849896 MsaT033633.1:CDS
AAACATGCGAACAGAGGCTT+TGG 0.435383 6:+95849838 MsaT033633.1:CDS
CAGAATGGAGGCCTGCAAAC+TGG 0.440962 6:+95854795 MsaT033633.1:CDS
ATGACATGTCAAAAGAAGAA+TGG 0.445126 6:+95855534 MsaT033633.1:CDS
CGAATACTGAGATGCAGAAA+TGG 0.448001 6:+95849976 MsaT033633.1:CDS
ACCTACCAAGCCTAAATTAG+TGG 0.455934 6:+95850689 MsaT033633.1:CDS
GTTCTTCAGGCTGCAAATGC+GGG 0.457663 6:+95849299 MsaT033633.1:CDS
TGACATGTCAAAAGAAGAAT+GGG 0.471345 6:+95855535 MsaT033633.1:CDS
TAGACTGAGAAATGTGGATC+TGG 0.471672 6:+95849643 MsaT033633.1:intron
ATTTCTGCATCTCAGTATTC+GGG 0.478549 6:-95849974 None:intergenic
TCTGGACAGGTTTATCAGAA+TGG 0.486271 6:+95854780 MsaT033633.1:intron
TGCAAGTCGTGATATAAAGA+AGG 0.505376 6:+95850176 MsaT033633.1:CDS
ATATGCCACTAATTTAGGCT+TGG 0.510433 6:-95850694 None:intergenic
AATTGAGTAAGCAATTTGAA+TGG 0.521092 6:-95854963 None:intergenic
TTTCAGATCATGTTTGAGGA+TGG 0.537141 6:+95855497 MsaT033633.1:intron
GTAATTGAATATGTCGGAGA+AGG 0.545438 6:+95849703 MsaT033633.1:CDS
TGTTCTTCAGGCTGCAAATG+CGG 0.552563 6:+95849298 MsaT033633.1:CDS
GCAGATGAAGATATAAAGCA+TGG 0.559769 6:+95849673 MsaT033633.1:CDS
ATAGATGGTGAAACTAGAAT+CGG 0.561402 6:+95850147 MsaT033633.1:CDS
GCCACTAATTTAGGCTTGGT+AGG 0.568833 6:-95850690 None:intergenic
GTTATTATGATAAAACAACT+TGG 0.570866 6:+95854997 MsaT033633.1:CDS
ACTGTGGTGCTGTTCAATGT+AGG 0.576878 6:+95850649 MsaT033633.1:CDS
TCTGATTCTACTGCTGTATG+TGG 0.586087 6:+95849100 MsaT033633.1:CDS
GAGTTTGTAATTGAATATGT+CGG 0.587237 6:+95849697 MsaT033633.1:CDS
CAGATGAAGATATAAAGCAT+GGG 0.595965 6:+95849674 MsaT033633.1:CDS
GTTCAATGTAGGCGTAAGCT+GGG 0.608277 6:+95850660 MsaT033633.1:CDS
CTTTGGAATATGAAAGATCG+TGG 0.610205 6:+95849855 MsaT033633.1:CDS
GCAAGTCGTGATATAAAGAA+GGG 0.610253 6:+95850177 MsaT033633.1:CDS
TTTGGAATATGAAAGATCGT+GGG 0.627929 6:+95849856 MsaT033633.1:CDS
ATGATAAAACATGCGAACAG+AGG 0.637191 6:+95849832 MsaT033633.1:CDS
TAAAACAACTTGGAAATGTG+AGG 0.644254 6:+95855007 MsaT033633.1:CDS
GTGGTAGAGATTGCCACTGT+GGG 0.651295 6:+95849119 MsaT033633.1:CDS
GGACAGGTTTATCAGAATGG+AGG 0.664470 6:+95854783 MsaT033633.1:intron
TGAATATTCTCGAAAACACA+AGG 0.669513 6:+95855182 MsaT033633.1:CDS
GAAATGTGGATCTGGAATTG+TGG 0.670067 6:+95849651 MsaT033633.1:CDS
AGATGAAGATATAAAGCATG+GGG 0.676792 6:+95849675 MsaT033633.1:CDS
TGTGGTAGAGATTGCCACTG+TGG 0.693149 6:+95849118 MsaT033633.1:CDS
TTGGAATATGAAAGATCGTG+GGG 0.693630 6:+95849857 MsaT033633.1:CDS
GCAGATCAAGATTGCCACTG+TGG 0.694777 6:+95850633 MsaT033633.1:CDS
TACATTGAACAGCACCACAG+TGG 0.726690 6:-95850647 None:intergenic
ACTTAGTTACTTACCCACAG+TGG 0.732787 6:-95849132 None:intergenic
TGCTTGTAGACTGAGAAATG+TGG 0.734376 6:+95849637 MsaT033633.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 95849074 95855574 95849074 ID=MsaG033633
Chr6 mRNA 95849074 95855574 95849074 ID=MsaT033633.1;Parent=MsaG033633
Chr6 exon 95849074 95849144 95849074 ID=MsaT033633.1.exon1;Parent=MsaT033633.1
Chr6 CDS 95849074 95849144 95849074 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95849282 95849381 95849282 ID=MsaT033633.1.exon2;Parent=MsaT033633.1
Chr6 CDS 95849282 95849381 95849282 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95849646 95849724 95849646 ID=MsaT033633.1.exon3;Parent=MsaT033633.1
Chr6 CDS 95849646 95849724 95849646 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95849826 95849997 95849826 ID=MsaT033633.1.exon4;Parent=MsaT033633.1
Chr6 CDS 95849826 95849997 95849826 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95850143 95850223 95850143 ID=MsaT033633.1.exon5;Parent=MsaT033633.1
Chr6 CDS 95850143 95850223 95850143 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95850617 95850719 95850617 ID=MsaT033633.1.exon6;Parent=MsaT033633.1
Chr6 CDS 95850617 95850719 95850617 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95854789 95854816 95854789 ID=MsaT033633.1.exon7;Parent=MsaT033633.1
Chr6 CDS 95854789 95854816 95854789 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95854944 95855028 95854944 ID=MsaT033633.1.exon8;Parent=MsaT033633.1
Chr6 CDS 95854944 95855028 95854944 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95855158 95855203 95855158 ID=MsaT033633.1.exon9;Parent=MsaT033633.1
Chr6 CDS 95855158 95855203 95855158 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Chr6 exon 95855503 95855574 95855503 ID=MsaT033633.1.exon10;Parent=MsaT033633.1
Chr6 CDS 95855503 95855574 95855503 ID=cds.MsaT033633.1;Parent=MsaT033633.1
Gene Sequence

>MsaT033633.1

ATGGTATATTCTGTTCTTGCGCATCTTCTGATTCTACTGCTGTATGTGGTAGAGATTGCCACTGTGGGTAAGATGCTTCTGTCTAGCTGTTCTTCAGGCTGCAAATGCGGGATCTCATGTCTCAACAAAGCTTTTCAGCACCGTCCTATTAAAAAGATGAAATTAATCAAGACTGAGAAATGTGGATCTGGAATTGTGGCAGATGAAGATATAAAGCATGGGGAGTTTGTAATTGAATATGTCGGAGAAGTTATCGATGATAAAACATGCGAACAGAGGCTTTGGAATATGAAAGATCGTGGGGAAACAAACTTCTACTTATGTGAAATCAATCGAGATATGGTGATAGATGCGACAAATAAAGGAAACAAGTCAAGATACATCAACCATAGTTGCTGCCCGAATACTGAGATGCAGAAATGGATAATAGATGGTGAAACTAGAATCGGCATATTTGCAAGTCGTGATATAAAGAAGGGCGAACATCTGACTTACGACTACCAGTTTGTTCAATTTGGTGCAGATCAAGATTGCCACTGTGGTGCTGTTCAATGTAGGCGTAAGCTGGGTGTGCGACCTACCAAGCCTAAATTAGTGGCATATCAGGTTTATCAGAATGGAGGCCTGCAAACTGGTGGTTCTCGTGTTGTTGACCATTCAAATTGCTTACTCAATTGCATCGATGAAGTTATTATGATAAAACAACTTGGAAATGTGAGATTTGGGATTATTAAATGGTTCGATGAATATTCTCGAAAACACAAGATCATGTTTGAGGATGGTTGTGTTGAAATCTATGACATGTCAAAAGAAGAATGGGAATTAGTGAGATTGTGA

Protein sequence

>MsaT033633.1

MVYSVLAHLLILLLYVVEIATVGKMLLSSCSSGCKCGISCLNKAFQHRPIKKMKLIKTEKCGSGIVADEDIKHGEFVIEYVGEVIDDKTCEQRLWNMKDRGETNFYLCEINRDMVIDATNKGNKSRYINHSCCPNTEMQKWIIDGETRIGIFASRDIKKGEHLTYDYQFVQFGADQDCHCGAVQCRRKLGVRPTKPKLVAYQVYQNGGLQTGGSRVVDHSNCLLNCIDEVIMIKQLGNVRFGIIKWFDEYSRKHKIMFEDGCVEIYDMSKEEWELVRL*