AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033793


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033793 MtrunA17_Chr6g0478581 96.970 429 13 0 1 429 1 429 0.0 846
MsaG033793 MtrunA17_Chr1g0161031 30.714 420 181 15 1 394 1 336 4.65e-32 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033793 AT3G17860.1 39.706 408 153 19 1 391 1 332 8.71e-55 186
MsaG033793 AT3G17860.2 38.978 372 141 17 34 391 20 319 1.87e-44 158
MsaG033793 AT3G17860.3 38.770 374 137 18 35 391 1 299 6.11e-44 156
MsaG033793 AT3G17860.4 38.770 374 137 18 35 391 1 299 6.11e-44 156
MsaG033793 AT1G48500.1 33.732 418 132 18 1 396 1 295 6.55e-38 140
MsaG033793 AT1G48500.2 33.333 399 122 17 1 378 1 276 3.50e-33 126
MsaG033793 AT1G48500.3 32.143 364 108 14 35 378 2 246 2.22e-27 109
MsaG033793 AT1G70700.2 41.593 113 58 4 125 237 58 162 1.21e-12 67.8
MsaG033793 AT1G70700.1 41.593 113 58 4 125 237 58 162 1.96e-12 67.8
MsaG033793 AT1G70700.3 41.593 113 58 4 125 237 58 162 2.72e-12 67.4

Find 98 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATACATGTTCTTCTTAA+AGG 0.090775 6:+99357491 None:intergenic
CTTGTTTCCTACTTGCTTTA+TGG 0.147271 6:+99359391 None:intergenic
TGTTCTTCTTAAAGGCAAAT+TGG 0.268291 6:+99357499 None:intergenic
GTCTAATCATAAATCATTCT+TGG 0.289760 6:-99361261 MsaT033793.1:CDS
TTTGATGCAGTTAGGAGTTC+AGG 0.316880 6:-99361434 MsaT033793.1:intron
GTTACAAACTCACAAACTTT+TGG 0.317063 6:-99359242 MsaT033793.1:CDS
AAATGCTTCTACTAACCATT+TGG 0.323976 6:-99358298 MsaT033793.1:CDS
AATCTCTATTCCTTCCAAAA+AGG 0.344054 6:-99358601 MsaT033793.1:CDS
AACTGTACCACCATAAAATA+TGG 0.350649 6:+99358864 None:intergenic
AAAACGATACCTTGGTCCTT+TGG 0.357357 6:+99365313 None:intergenic
GTTGGAAGCATCTTCTTTCA+TGG 0.359919 6:+99365339 None:intergenic
AGTTTCTCCATTGATTTCTC+TGG 0.361158 6:-99357542 MsaT033793.1:CDS
ACCTTGGTCCTTTGGTTTGT+TGG 0.375341 6:+99365321 None:intergenic
CCTGGTTGAATCACATTTGT+AGG 0.375793 6:+99358240 None:intergenic
CGGTGTAGTACCATTGGATT+TGG 0.378173 6:+99358897 None:intergenic
TTTGAGGGAAGAACAGATAA+AGG 0.390189 6:+99359200 None:intergenic
GTCACAATAATAAGATCTTT+AGG 0.396082 6:-99358327 MsaT033793.1:CDS
TCTGATCCAGTGCTGCATTC+AGG 0.408286 6:+99357572 None:intergenic
CAATCTAATCATGTTACTAC+TGG 0.412033 6:-99359284 MsaT033793.1:CDS
TGAGAGGACTTTGCAATTGA+AGG 0.412113 6:+99358447 None:intergenic
TAAGTCAGTAGAGCCAACTA+TGG 0.416073 6:+99359172 None:intergenic
GTTGCTACTTGAACCTCCCT+TGG 0.419319 6:+99358356 None:intergenic
TAGTTGGCTCTACTGACTTA+AGG 0.429024 6:-99359169 MsaT033793.1:intron
CAGTAGTAACATGATTAGAT+TGG 0.431253 6:+99359285 None:intergenic
TTATCTGTTCTTCCCTCAAA+AGG 0.432397 6:-99359197 MsaT033793.1:CDS
CCTATTGTTGCTACTTCTGT+AGG 0.433479 6:-99358270 MsaT033793.1:CDS
CTGATTGTGCATCAGAACTT+TGG 0.440135 6:+99359345 None:intergenic
CCTACAGAAGTAGCAACAAT+AGG 0.446555 6:+99358270 None:intergenic
GAAGTCATTGGGAGAGAACT+TGG 0.446941 6:+99358396 None:intergenic
GAACTTGCATGAGAAGTCAT+TGG 0.447319 6:+99358384 None:intergenic
TTGCTACTTGATCCTCCCCT+TGG 0.449675 6:+99358501 None:intergenic
GCCTTTAAGAAGAACATGTA+TGG 0.452229 6:-99357492 MsaT033793.1:CDS
GCTTGGCAATGGTGAGGGAT+AGG 0.455476 6:+99358557 None:intergenic
TTGCTACTTGAACCTCCCTT+GGG 0.466052 6:+99358357 None:intergenic
GAGGTCATTGGAAGAGGGCT+TGG 0.466655 6:+99358540 None:intergenic
GTACTTGCATGAGAGGTCAT+TGG 0.470357 6:+99358528 None:intergenic
TCTAATCATAAATCATTCTT+GGG 0.475638 6:-99361260 MsaT033793.1:CDS
GTTTCCAGTTGGACTGCCTC+AGG 0.477866 6:-99358001 MsaT033793.1:intron
AGAATAACACTCATGAAGAT+AGG 0.479954 6:-99361355 MsaT033793.1:CDS
CCCTCAAAAGGTCCCATAGT+TGG 0.482615 6:-99359185 MsaT033793.1:CDS
AACTTGCATGAGAAGTCATT+GGG 0.486290 6:+99358385 None:intergenic
ATATATCCTGATGAAGCTAC+TGG 0.487462 6:+99361311 None:intergenic
TAAAATATGGTCAACTGAGC+CGG 0.491802 6:+99358877 None:intergenic
CTTGGCAATGGTGAGGGATA+GGG 0.492080 6:+99358558 None:intergenic
GCATGAGAGGTCATTGGAAG+AGG 0.495990 6:+99358534 None:intergenic
ATGGATCCAGTAGCTTCATC+AGG 0.498303 6:-99361317 MsaT033793.1:CDS
ATCATGTTACTAGCTGGAAA+TGG 0.499618 6:-99358642 MsaT033793.1:CDS
CTGAGCCGGTGTAGTACCAT+TGG 0.502183 6:+99358891 None:intergenic
GTCACAATAATAAGATCCTT+AGG 0.503685 6:-99358471 MsaT033793.1:CDS
AACTTGGCAAAGGTGAGAGA+TGG 0.504464 6:+99358412 None:intergenic
TTCTTGAATGACATGAATTG+AGG 0.505348 6:+99361374 None:intergenic
ATCATTCTTGGGTGTGACAC+AGG 0.505809 6:-99361249 MsaT033793.1:intron
CAACAATAGGAGATTCCAAA+TGG 0.511822 6:+99358283 None:intergenic
CTTTGCAATTGAAGGTCCTA+AGG 0.512362 6:+99358455 None:intergenic
TAGGTCAAGAAACAGTGTTA+TGG 0.516069 6:-99361336 MsaT033793.1:CDS
AACAGATAAAGGTGTGGCAG+AGG 0.516274 6:+99359211 None:intergenic
AAGGCAATCATGTTACTAGC+TGG 0.519440 6:-99358648 MsaT033793.1:CDS
TCATGCAAGTTCTTATCCCA+AGG 0.526316 6:-99358373 MsaT033793.1:CDS
CTTCTTAAAGGCAAATTGGT+GGG 0.529876 6:+99357503 None:intergenic
TCTTCTTTCATGGTAGTCCA+TGG 0.530904 6:+99365349 None:intergenic
AAATCACCTGAATGCAGCAC+TGG 0.539481 6:-99357578 MsaT033793.1:CDS
ACTTGGCAAAGGTGAGAGAT+GGG 0.542203 6:+99358413 None:intergenic
ACTTGAACCAGAGAAATCAA+TGG 0.546674 6:+99357535 None:intergenic
AGAGGGCTTGGCAATGGTGA+GGG 0.558559 6:+99358552 None:intergenic
TGGTTGAGTACTTGCATGAG+AGG 0.558968 6:+99358521 None:intergenic
TCTTCTTAAAGGCAAATTGG+TGG 0.560082 6:+99357502 None:intergenic
TGGTTAGTAGAAGCATTTGA+AGG 0.561148 6:+99358303 None:intergenic
CATATGGAAAGAGAATTCCT+TGG 0.576225 6:-99365391 MsaT033793.1:CDS
CATGAGAGGTCATTGGAAGA+GGG 0.576369 6:+99358535 None:intergenic
TGAATGACATGAATTGAGGA+AGG 0.578633 6:+99361378 None:intergenic
ATTGGAAGAGGGCTTGGCAA+TGG 0.579330 6:+99358546 None:intergenic
TTGTGCATCAGAACTTTGGA+TGG 0.580883 6:+99359349 None:intergenic
AAGAGGGCTTGGCAATGGTG+AGG 0.587772 6:+99358551 None:intergenic
CTTAAAGGCAAATTGGTGGG+TGG 0.595542 6:+99357506 None:intergenic
CGCGAAAAGCATCGTTAACG+CGG 0.596341 6:-99357978 MsaT033793.1:CDS
CCTACAAATGTGATTCAACC+AGG 0.596560 6:-99358240 MsaT033793.1:intron
TCATGCAAGTACTCAACCAA+GGG 0.597542 6:-99358517 MsaT033793.1:CDS
AAGTCAGTAGAGCCAACTAT+GGG 0.597778 6:+99359173 None:intergenic
CATGCAAGTTCTTATCCCAA+GGG 0.608931 6:-99358372 MsaT033793.1:CDS
TGATGACATCTCTCCTGAGA+AGG 0.609439 6:-99358833 MsaT033793.1:intron
CTCATGCAAGTACTCAACCA+AGG 0.611643 6:-99358518 MsaT033793.1:CDS
AGGAATTGTTCCAAATCCAA+TGG 0.614409 6:-99358907 MsaT033793.1:CDS
ATGGGATAGGTCATTGTGAG+AGG 0.619583 6:+99358431 None:intergenic
GCAAAGGTGAGAGATGGGAT+AGG 0.630123 6:+99358418 None:intergenic
AATTTGGCCATAAAGCAAGT+AGG 0.634556 6:-99359398 MsaT033793.1:CDS
GCAATGGTGAGGGATAGGGT+TGG 0.638300 6:+99358562 None:intergenic
AAGCAAGTAGGAAACAAGCA+TGG 0.639462 6:-99359386 MsaT033793.1:CDS
GCAAGTTCTTATCCCAAGGG+AGG 0.641995 6:-99358369 MsaT033793.1:CDS
TGTGCATCAGAACTTTGGAT+GGG 0.644773 6:+99359350 None:intergenic
ATTGGGAGAGAACTTGGCAA+AGG 0.651165 6:+99358402 None:intergenic
CAAATCCAATGGTACTACAC+CGG 0.651773 6:-99358896 MsaT033793.1:CDS
AGATGCTTCCAACAAACCAA+AGG 0.662188 6:-99365329 MsaT033793.1:CDS
TCCAACAAACCAAAGGACCA+AGG 0.670257 6:-99365322 MsaT033793.1:intron
GCAAGTACTCAACCAAGGGG+AGG 0.678862 6:-99358513 MsaT033793.1:CDS
TTAGGAGTTCAGGAATGCAG+TGG 0.686957 6:-99361424 MsaT033793.1:CDS
TGTTACTACTGGAATCAACA+TGG 0.687462 6:-99359273 MsaT033793.1:CDS
GGGAAGAACAGATAAAGGTG+TGG 0.711636 6:+99359205 None:intergenic
CATGCAAGTACTCAACCAAG+GGG 0.756359 6:-99358516 MsaT033793.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 99357478 99365410 99357478 ID=MsaG033793
Chr6 mRNA 99357478 99365410 99357478 ID=MsaT033793.1;Parent=MsaG033793
Chr6 exon 99357478 99357634 99357478 ID=MsaT033793.1.exon7;Parent=MsaT033793.1
Chr6 CDS 99357478 99357634 99357478 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99357955 99358015 99357955 ID=MsaT033793.1.exon6;Parent=MsaT033793.1
Chr6 CDS 99357955 99358015 99357955 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99358241 99358673 99358241 ID=MsaT033793.1.exon5;Parent=MsaT033793.1
Chr6 CDS 99358241 99358673 99358241 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99358834 99358933 99358834 ID=MsaT033793.1.exon4;Parent=MsaT033793.1
Chr6 CDS 99358834 99358933 99358834 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99359170 99359423 99359170 ID=MsaT033793.1.exon3;Parent=MsaT033793.1
Chr6 CDS 99359170 99359423 99359170 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99361250 99361446 99361250 ID=MsaT033793.1.exon2;Parent=MsaT033793.1
Chr6 CDS 99361250 99361446 99361250 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Chr6 exon 99365323 99365410 99365323 ID=MsaT033793.1.exon1;Parent=MsaT033793.1
Chr6 CDS 99365323 99365410 99365323 ID=cds.MsaT033793.1;Parent=MsaT033793.1
Gene Sequence

>MsaT033793.1

ATGGAAAGAGAATTCCTTGGCTTCAGTTCCAAAAATAGTCCATGGACTACCATGAAAGAAGATGCTTCCAACAAACCAAAGGACCAAGTTAGGAGTTCAGGAATGCAGTGGTCATTTTCAAATAAGGTTTCAAACCTTCCTCAATTCATGTCATTCAAGAATAACACTCATGAAGATAGGTCAAGAAACAGTGTTATGGATCCAGTAGCTTCATCAGGATATATGACTATTTCAACTAAAGATGCTTTTGAGTCTAATCATAAATCATTCTTGGGTGTGACACAGGAAAATTTGGCCATAAAGCAAGTAGGAAACAAGCATGGAATTACAATTTATCCCATCCAAAGTTCTGATGCACAATCAGTTTGCAATCAAGAAGCAAGAACATTTTCAGTTTCAAACCAATCTAATCATGTTACTACTGGAATCAACATGGTTAATTCAGTTACAAACTCACAAACTTTTGGTTCAAAATCCTCTGCCACACCTTTATCTGTTCTTCCCTCAAAAGGTCCCATAGTTGGCTCTACTGACTTAAGGAGTAGGAATTGTTCCAAATCCAATGGTACTACACCGGCTCAGTTGACCATATTTTATGGTGGTACAGTTTGTGTGTATGATGACATCTCTCCTGAGAAGGCTAAGGCAATCATGTTACTAGCTGGAAATGGCACTAAAATGCAGCAAGAAATCTCTATTCCTTCCAAAAAGGATAATTTCATTATAAGCCAACCCTATCCCTCACCATTGCCAAGCCCTCTTCCAATGACCTCTCATGCAAGTACTCAACCAAGGGGAGGATCAAGTAGCAACAATGAAGTCACAATAATAAGATCCTTAGGACCTTCAATTGCAAAGTCCTCTCACAATGACCTATCCCATCTCTCACCTTTGCCAAGTTCTCTCCCAATGACTTCTCATGCAAGTTCTTATCCCAAGGGAGGTTCAAGTAGCAACAATGAAGTCACAATAATAAGATCTTTAGGACCTTCAAATGCTTCTACTAACCATTTGGAATCTCCTATTGTTGCTACTTCTGTAGGATCAACACCTACAAATGTGATTCAACCAGTTGGACTGCCTCAGGCGCGAAAAGCATCGTTAACGCGGTTTTTGGAGAAGCGAAAGGAAAGGGCAATGAGCACATCACCATACTATATGAGCAAAAAATCACCTGAATGCAGCACTGGATCAGACAATGCTAGTTTCTCCATTGATTTCTCTGGTTCAAGTACTCAACCACCCACCAATTTGCCTTTAAGAAGAACATGTATGGAAGTGATTAGATAA

Protein sequence

>MsaT033793.1

MEREFLGFSSKNSPWTTMKEDASNKPKDQVRSSGMQWSFSNKVSNLPQFMSFKNNTHEDRSRNSVMDPVASSGYMTISTKDAFESNHKSFLGVTQENLAIKQVGNKHGITIYPIQSSDAQSVCNQEARTFSVSNQSNHVTTGINMVNSVTNSQTFGSKSSATPLSVLPSKGPIVGSTDLRSRNCSKSNGTTPAQLTIFYGGTVCVYDDISPEKAKAIMLLAGNGTKMQQEISIPSKKDNFIISQPYPSPLPSPLPMTSHASTQPRGGSSSNNEVTIIRSLGPSIAKSSHNDLSHLSPLPSSLPMTSHASSYPKGGSSSNNEVTIIRSLGPSNASTNHLESPIVATSVGSTPTNVIQPVGLPQARKASLTRFLEKRKERAMSTSPYYMSKKSPECSTGSDNASFSIDFSGSSTQPPTNLPLRRTCMEVIR*