Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033820 | XP_003619882.2 | 98.457 | 324 | 5 | 0 | 1 | 324 | 1 | 324 | 0.0 | 668 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033820 | sp|Q9FX43|M2K9_ARATH | 58.769 | 325 | 116 | 5 | 1 | 322 | 1 | 310 | 2.12e-120 | 351 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033820 | tr|G7KQC0|G7KQC0_MEDTR | 98.457 | 324 | 5 | 0 | 1 | 324 | 1 | 324 | 0.0 | 668 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033820 | MtrunA17_Chr6g0479211 | 98.457 | 324 | 5 | 0 | 1 | 324 | 1 | 324 | 0.0 | 668 |
| MsaG033820 | MtrunA17_Chr4g0000481 | 44.828 | 319 | 165 | 6 | 6 | 321 | 32 | 342 | 7.14e-92 | 278 |
| MsaG033820 | MtrunA17_Chr6g0487231 | 42.751 | 269 | 142 | 7 | 57 | 320 | 70 | 331 | 4.00e-64 | 206 |
| MsaG033820 | MtrunA17_Chr4g0070851 | 42.629 | 251 | 134 | 6 | 73 | 318 | 87 | 332 | 4.71e-56 | 186 |
| MsaG033820 | MtrunA17_Chr2g0299991 | 38.909 | 275 | 160 | 5 | 49 | 318 | 63 | 334 | 6.19e-56 | 185 |
| MsaG033820 | MtrunA17_Chr6g0449691 | 40.824 | 267 | 142 | 9 | 59 | 320 | 87 | 342 | 2.44e-51 | 177 |
| MsaG033820 | MtrunA17_Chr5g0424581 | 35.055 | 271 | 161 | 7 | 51 | 318 | 120 | 378 | 3.67e-38 | 139 |
| MsaG033820 | MtrunA17_Chr5g0424501 | 35.055 | 271 | 161 | 7 | 51 | 318 | 187 | 445 | 2.00e-37 | 139 |
| MsaG033820 | MtrunA17_Chr3g0130151 | 32.963 | 270 | 165 | 6 | 54 | 318 | 242 | 500 | 4.83e-37 | 140 |
| MsaG033820 | MtrunA17_Chr3g0133541 | 33.230 | 322 | 178 | 13 | 18 | 318 | 263 | 568 | 2.05e-36 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033820 | AT1G73500.1 | 58.769 | 325 | 116 | 5 | 1 | 322 | 1 | 310 | 2.15e-121 | 351 |
| MsaG033820 | AT1G18350.1 | 54.829 | 321 | 124 | 7 | 1 | 318 | 1 | 303 | 9.29e-116 | 337 |
| MsaG033820 | AT3G21220.2 | 50.909 | 275 | 124 | 5 | 50 | 320 | 60 | 327 | 5.69e-94 | 283 |
| MsaG033820 | AT3G21220.1 | 50.909 | 275 | 124 | 5 | 50 | 320 | 60 | 327 | 5.69e-94 | 283 |
| MsaG033820 | AT1G51660.1 | 51.128 | 266 | 123 | 4 | 55 | 320 | 78 | 336 | 8.39e-93 | 281 |
| MsaG033820 | AT3G06230.1 | 45.833 | 288 | 131 | 7 | 23 | 306 | 27 | 293 | 9.84e-82 | 250 |
| MsaG033820 | AT1G32320.1 | 45.522 | 268 | 136 | 5 | 52 | 318 | 44 | 302 | 3.80e-73 | 228 |
| MsaG033820 | AT4G29810.1 | 41.516 | 277 | 150 | 6 | 49 | 320 | 63 | 332 | 1.25e-63 | 206 |
| MsaG033820 | AT4G29810.2 | 41.516 | 277 | 150 | 6 | 49 | 320 | 72 | 341 | 4.17e-63 | 204 |
| MsaG033820 | AT5G56580.1 | 40.364 | 275 | 153 | 6 | 51 | 320 | 65 | 333 | 8.73e-63 | 203 |
Find 93 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGCAAACGTCGCCGGT+GGG | 0.601398 | 6:+100052407 | None:intergenic |
| AAACTCTCTGTCCTCGGCCA+TGG | 0.532287 | 6:-100052608 | MsaT033820.1:CDS |
| AACTCTGACGAAGCTGTCTC+AGG | 0.460599 | 6:+100051930 | None:intergenic |
| AAGCTCTCCACGGTGGCGCG+TGG | 0.571791 | 6:-100052317 | MsaT033820.1:CDS |
| AATATTGTCACCTCCGGCGA+CGG | 0.507149 | 6:+100052649 | None:intergenic |
| AATCGGGTCAACGACCCGAT+TGG | 0.513458 | 6:-100051998 | MsaT033820.1:CDS |
| AATTCCCTCCATAAACTTCT+GGG | 0.423320 | 6:+100052099 | None:intergenic |
| ACCAACGTCGTAAAATACCA+CGG | 0.733728 | 6:-100052443 | MsaT033820.1:CDS |
| ACCACCGCCTCCGTCGCCGG+AGG | 0.630233 | 6:-100052659 | MsaT033820.1:CDS |
| ACCCTATTTGAACTTTATGT+AGG | 0.506803 | 6:-100052038 | MsaT033820.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 100051824 | 100052801 | 100051824 | ID=MsaG033820 |
| Chr6 | mRNA | 100051824 | 100052801 | 100051824 | ID=MsaT033820.1;Parent=MsaG033820 |
| Chr6 | exon | 100051824 | 100052801 | 100051824 | ID=MsaT033820.1.exon1;Parent=MsaT033820.1 |
| Chr6 | CDS | 100051824 | 100052801 | 100051824 | ID=cds.MsaT033820.1;Parent=MsaT033820.1 |
| Gene Sequence |
>MsaT033820.1 ATGGCGCTTGTTCACCGCCGCAACAGCCCTAAACTTCGGCTACCTGAAATCTCCGACCACCGTCCTCGTTTCCCTGTTCCTCTTCCTGCAAACATCTACAAACAACCCTCCACCTCATCCACCACCGCCTCCGTCGCCGGAGGTGACAATATTACTGCCGGTGACTTTGAAAAACTCTCTGTCCTCGGCCATGGCAACGGTGGCACCGTCTACAAAGTCCGTCACAAACTCACCTCCATCATTTACGCTCTAAAAGCCAACCATTATGACTCTGACCCCACCACGCGCCGTCGTGCACTCACCGAAGTAAACATCCTCCGCCGTGCAACTGATTGCACCAACGTCGTAAAATACCACGGCTCCTTCGAGAAACCCACCGGCGACGTTTGCATTTTAATGGAATACATGGACTCCGGCAGCCTAGAAACCGCCCTCAAAACAACCGGCACATTCTCCGAATCAAAGCTCTCCACGGTGGCGCGTGGCATTTTAAACGGTCTCACGTACCTTCACGCGCGTAACATAGCGCATCGTGACATAAAACCTTCGAACATCCTCGTAAACATAAAAAACGAGGTTAAAATTGCTGATTTTGGTGTCAGTAAATTTATGGGTCGCACGCTTGAAGCATGTAACTCTTATGTTGGCACGTGTGCTTATATGAGTCCTGAAAGATTTGACCCAGAAGTTTATGGAGGGAATTATAACGGTTTTTCAGCTGATATATGGAGTTTGGGTTTAACCCTATTTGAACTTTATGTAGGTCATTTTCCATTTCTTCAATCGGGTCAACGACCCGATTGGGCTTCACTTATGTGCGCAATTTGTTTCTCTGACCCACCGAGTCTTCCTGAGACAGCTTCGTCAGAGTTTAGAAACTTTGTCGAATGTTGTCTTAAGAAGGAGTCTGGTGAACGGTGGTCGGCGGCTCAGTTGTTGACCCACCCGTTTTTGTGTAAAGATATGGAAAGTTGTTAA |
| Protein sequence |
>MsaT033820.1 MALVHRRNSPKLRLPEISDHRPRFPVPLPANIYKQPSTSSTTASVAGGDNITAGDFEKLSVLGHGNGGTVYKVRHKLTSIIYALKANHYDSDPTTRRRALTEVNILRRATDCTNVVKYHGSFEKPTGDVCILMEYMDSGSLETALKTTGTFSESKLSTVARGILNGLTYLHARNIAHRDIKPSNILVNIKNEVKIADFGVSKFMGRTLEACNSYVGTCAYMSPERFDPEVYGGNYNGFSADIWSLGLTLFELYVGHFPFLQSGQRPDWASLMCAICFSDPPSLPETASSEFRNFVECCLKKESGERWSAAQLLTHPFLCKDMESC* |
