AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033879

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033879 MtrunA17_Chr6g0479901 98.324 179 3 0 1 179 1 179 7.10e-134 371
MsaG033879 MtrunA17_Chr7g0217231 38.776 196 61 5 19 160 88 278 5.57e-39 135
MsaG033879 MtrunA17_Chr1g0188441 35.795 176 77 5 19 160 9 182 3.05e-34 119
MsaG033879 MtrunA17_Chr5g0412501 36.979 192 69 4 19 160 78 267 1.80e-32 117
MsaG033879 MtrunA17_Chr2g0315941 32.701 211 82 6 6 160 57 263 3.19e-31 114
MsaG033879 MtrunA17_Chr6g0453231 35.567 194 69 6 19 160 91 280 1.22e-29 111
MsaG033879 MtrunA17_Chr8g0367691 32.057 209 85 5 7 160 57 263 5.82e-29 107
MsaG033879 MtrunA17_Chr3g0133181 32.308 195 80 5 19 163 80 272 1.06e-26 102
MsaG033879 MtrunA17_Chr8g0361671 56.338 71 30 1 101 171 271 340 2.93e-22 92.0
MsaG033879 MtrunA17_Chr8g0361671 66.667 51 16 1 19 68 64 114 5.05e-17 77.4
MsaG033879 MtrunA17_Chr4g0076821 56.667 60 25 1 101 160 284 342 4.18e-20 85.9
MsaG033879 MtrunA17_Chr4g0076821 62.000 50 18 1 19 68 113 161 5.76e-15 71.6
MsaG033879 MtrunA17_Chr5g0404271 56.667 60 25 1 101 160 233 291 1.41e-19 83.6
MsaG033879 MtrunA17_Chr5g0404271 61.538 52 15 2 19 68 99 147 1.00e-15 73.2
MsaG033879 MtrunA17_Chr5g0427051 53.125 64 29 1 97 160 201 263 1.41e-16 75.1
MsaG033879 MtrunA17_Chr5g0427051 56.000 50 21 1 19 68 58 106 7.43e-12 62.0
MsaG033879 MtrunA17_Chr8g0357741 46.377 69 35 1 92 160 26 92 3.11e-16 70.5
MsaG033879 MtrunA17_Chr3g0092371 50.794 63 30 1 6 68 7 68 2.91e-15 67.4
MsaG033879 MtrunA17_Chr8g0357721 33.594 128 67 5 28 139 14 139 1.40e-14 67.4
MsaG033879 MtrunA17_Chr6g0453221 60.000 50 15 1 19 68 21 65 2.49e-14 65.1
MsaG033879 MtrunA17_Chr4g0060731 52.459 61 25 1 8 68 14 70 6.04e-13 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033879 AT1G14440.1 41.270 189 58 6 19 158 90 274 1.48e-40 139
MsaG033879 AT1G14440.2 41.270 189 58 6 19 158 90 274 1.48e-40 139
MsaG033879 AT2G02540.2 35.981 214 75 5 19 172 87 298 6.14e-40 137
MsaG033879 AT2G02540.1 35.981 214 75 5 19 172 87 298 6.14e-40 137
MsaG033879 AT4G24660.1 42.012 169 69 3 19 160 49 215 2.88e-39 133
MsaG033879 AT4G24660.2 42.012 169 69 3 19 160 49 215 3.70e-39 133
MsaG033879 AT2G18350.1 40.678 177 68 5 19 160 82 256 9.95e-39 133
MsaG033879 AT5G65410.1 39.779 181 68 5 17 160 73 249 2.07e-38 133
MsaG033879 AT1G69600.1 39.894 188 69 6 19 163 31 217 4.54e-35 123
MsaG033879 AT3G28920.1 38.500 200 64 5 19 160 52 250 7.13e-35 124
MsaG033879 AT5G15210.1 37.634 186 68 5 19 160 56 237 1.33e-34 123
MsaG033879 AT5G42780.1 37.368 190 79 4 6 159 51 236 3.84e-34 121
MsaG033879 AT5G60480.1 39.412 170 72 5 18 158 36 203 1.00e-32 117
MsaG033879 AT3G50890.1 38.220 191 76 4 19 169 60 248 7.97e-31 112
MsaG033879 AT1G14687.1 38.554 166 72 5 18 159 6 165 2.60e-30 108
MsaG033879 AT1G75240.1 60.000 70 23 2 101 170 240 304 1.24e-23 95.1
MsaG033879 AT1G75240.1 62.000 50 18 1 19 68 76 124 2.18e-14 70.1
MsaG033879 AT5G39760.1 54.839 62 27 1 99 160 198 258 3.77e-18 80.5
MsaG033879 AT5G39760.1 48.649 74 37 1 17 89 54 127 2.59e-15 72.8
MsaG033879 AT1G74660.2 56.604 53 22 1 16 68 36 87 2.97e-15 68.2
MsaG033879 AT1G74660.1 56.604 53 22 1 16 68 36 87 6.72e-15 67.4
MsaG033879 AT3G28917.1 54.000 50 23 0 19 68 33 82 5.70e-14 65.1
MsaG033879 AT1G18835.1 54.717 53 20 1 16 68 23 71 2.38e-13 63.2

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAGGTAGTGGAGGAGA+TGG 0.351040 6:+101354888 MsaT033879.1:CDS
ATGGGATTTGCTGAGAAGTT+AGG 0.355084 6:+101354963 MsaT033879.1:CDS
GAGGAGATGGGAGGAGGAAG+AGG 0.371577 6:+101354901 MsaT033879.1:CDS
GAATTAGCATTACCAGTAGA+AGG 0.429521 6:-101355116 None:intergenic
GAGGAGGTAGTGGAGGAGAT+GGG 0.433503 6:+101354889 MsaT033879.1:CDS
GAGGTAGTGGAGGAGATGGG+AGG 0.435653 6:+101354892 MsaT033879.1:CDS
GTAGTGGAGGAGATGGGAGG+AGG 0.441484 6:+101354895 MsaT033879.1:CDS
TGCCGCGGCCGCTGCAATGT+CGG 0.446748 6:+101354842 MsaT033879.1:CDS
ATCATCTTCTCCTTCTGATC+CGG 0.451236 6:-101354942 None:intergenic
TATTCCGGTGGAGGAGGTAG+TGG 0.451652 6:+101354879 MsaT033879.1:CDS
AGCCGCAGGCTGCACATTGG+AGG 0.456002 6:-101354773 None:intergenic
GCAAAATAACCGCGATCACA+AGG 0.458670 6:+101354814 MsaT033879.1:CDS
AGAAACCATGCAGCAAGCCT+TGG 0.459741 6:+101354684 MsaT033879.1:CDS
CGTCGGTGGCGTAGCTTCCA+AGG 0.465781 6:-101354701 None:intergenic
GAAATTTACGCCGGATCAGA+AGG 0.471585 6:+101354932 MsaT033879.1:CDS
ATCCGACATTGCAGCGGCCG+CGG 0.471777 6:-101354844 None:intergenic
AATTCTTCTGATCCTTCTAC+TGG 0.475357 6:+101355104 MsaT033879.1:CDS
GATCAGTTGATGGAATATTC+CGG 0.475527 6:+101354864 MsaT033879.1:CDS
AGCTTCCAAGGCTTGCTGCA+TGG 0.476710 6:-101354689 None:intergenic
GAAGTTCCGGTGACAGCCGC+AGG 0.479614 6:-101354787 None:intergenic
TGGGATTTGCTGAGAAGTTA+GGG 0.491743 6:+101354964 MsaT033879.1:CDS
ATGGGAGGAGGAAGAGGTAC+CGG 0.496163 6:+101354907 MsaT033879.1:CDS
TGCAATGTCGGATCAGTTGA+TGG 0.497838 6:+101354854 MsaT033879.1:CDS
GTGCAGCCTGCGGCTGTCAC+CGG 0.504708 6:+101354781 MsaT033879.1:CDS
GGATCAGAAGGAGAAGATGA+TGG 0.523708 6:+101354944 MsaT033879.1:CDS
AACTTGATGAAGAGATTGAA+AGG 0.524348 6:+101355006 MsaT033879.1:CDS
TCCTCCACTACCTCCTCCAC+CGG 0.526819 6:-101354883 None:intergenic
GATCCGGCGTAAATTTCGTC+CGG 0.537660 6:-101354926 None:intergenic
ATGGAATATTCCGGTGGAGG+AGG 0.538823 6:+101354873 MsaT033879.1:CDS
AGCTACGCCACCGACGGCTG+CGG 0.539403 6:+101354708 MsaT033879.1:CDS
TGGAGGCTGTTTATCGCGGA+TGG 0.549396 6:-101354756 None:intergenic
GAATTCACCGCAGCCGTCGG+TGG 0.562401 6:-101354715 None:intergenic
GGTGAATTCACCGCAGCCGT+CGG 0.578066 6:-101354718 None:intergenic
GACAGCCGCAGGCTGCACAT+TGG 0.578845 6:-101354776 None:intergenic
GGCACTCTCCCTTGTGATCG+CGG 0.582002 6:-101354823 None:intergenic
GATCAGAAGGAGAAGATGAT+GGG 0.588034 6:+101354945 MsaT033879.1:CDS
TCCGGTGGAGGAGGTAGTGG+AGG 0.589804 6:+101354882 MsaT033879.1:CDS
TTGATGGAATATTCCGGTGG+AGG 0.592071 6:+101354870 MsaT033879.1:CDS
CAAAATAACCGCGATCACAA+GGG 0.605300 6:+101354815 MsaT033879.1:CDS
GGATGGCGATGAGTCGTCGA+TGG 0.613786 6:-101354739 None:intergenic
CACACACTACTGAGTAAGAG+AGG 0.616345 6:-101355138 None:intergenic
AGCCTCCAATGTGCAGCCTG+CGG 0.622915 6:+101354771 MsaT033879.1:CDS
GATCACAAGGGAGAGTGCCG+CGG 0.625206 6:+101354827 MsaT033879.1:CDS
ACATTGGAGGCTGTTTATCG+CGG 0.631854 6:-101354760 None:intergenic
CTTGGAAGCTACGCCACCGA+CGG 0.637476 6:+101354702 MsaT033879.1:CDS
GATTTGCTGAGAAGTTAGGG+TGG 0.665461 6:+101354967 MsaT033879.1:CDS
CAGTTGATGGAATATTCCGG+TGG 0.694003 6:+101354867 MsaT033879.1:CDS
CAACTGATCCGACATTGCAG+CGG 0.694689 6:-101354850 None:intergenic
GTACCGGACGAAATTTACGC+CGG 0.738784 6:+101354923 MsaT033879.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 101354615 101355154 101354615 ID=MsaG033879
Chr6 mRNA 101354615 101355154 101354615 ID=MsaT033879.1;Parent=MsaG033879
Chr6 exon 101354615 101355154 101354615 ID=MsaT033879.1.exon1;Parent=MsaT033879.1
Chr6 CDS 101354615 101355154 101354615 ID=cds.MsaT033879.1;Parent=MsaT033879.1
Gene Sequence

>MsaT033879.1

ATGGAAGGAGGAGGAATGTTATCATCTTCATCATCACATTCACAAAACTACATCTACAGAGAGTGTTTAAGAAACCATGCAGCAAGCCTTGGAAGCTACGCCACCGACGGCTGCGGTGAATTCACCATCGACGACTCATCGCCATCCGCGATAAACAGCCTCCAATGTGCAGCCTGCGGCTGTCACCGGAACTTCCACCGCAAAATAACCGCGATCACAAGGGAGAGTGCCGCGGCCGCTGCAATGTCGGATCAGTTGATGGAATATTCCGGTGGAGGAGGTAGTGGAGGAGATGGGAGGAGGAAGAGGTACCGGACGAAATTTACGCCGGATCAGAAGGAGAAGATGATGGGATTTGCTGAGAAGTTAGGGTGGAAATTGCAGAGAAAAGAACTTGATGAAGAGATTGAAAGGTTTTGTGAAAGTGTTGGTGTGAGTAGACAAGTTTTTAAGGTTTGGATGCATAATCATAAGAATTCTTGTTTCTCAAATTCTTCTGATCCTTCTACTGGTAATGCTAATTCCTCTCTTACTCAGTAG
Protein sequence

>MsaT033879.1

MEGGGMLSSSSSHSQNYIYRECLRNHAASLGSYATDGCGEFTIDDSSPSAINSLQCAACGCHRNFHRKITAITRESAAAAAMSDQLMEYSGGGGSGGDGRRKRYRTKFTPDQKEKMMGFAEKLGWKLQRKELDEEIERFCESVGVSRQVFKVWMHNHKNSCFSNSSDPSTGNANSSLTQ*