AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033883


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033883 MtrunA17_Chr6g0479551 87.732 269 27 1 1 269 1 263 9.06e-177 487
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033883 AT1G18335.3 50.566 265 119 4 3 263 6 262 2.12e-77 236
MsaG033883 AT1G18335.2 50.566 265 119 4 3 263 6 262 2.12e-77 236
MsaG033883 AT1G18335.1 50.379 264 119 4 3 262 6 261 4.33e-77 235

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTATATATTGTTTAAT+AGG 0.217352 6:-101386939 None:intergenic
TGTACATTACCGATTTGTTT+TGG 0.337734 6:+101388007 MsaT033883.1:CDS
ACCATTGGATAAACTTTAGA+AGG 0.346375 6:-101389198 None:intergenic
ATTTCGAAGCAGCGATAAAC+GGG 0.378315 6:-101385540 None:intergenic
GATTTCGAAGCAGCGATAAA+CGG 0.401891 6:-101385541 None:intergenic
CGTGTCCAGGGGAAAGGATT+GGG 0.426522 6:+101388077 MsaT033883.1:CDS
CAAAGCAGCATATAGCATAA+AGG 0.436019 6:+101385500 MsaT033883.1:CDS
ACCAAGGGACCACTATCTTC+TGG 0.440378 6:-101387978 None:intergenic
ATATATACAAAGACTTCTTA+AGG 0.442688 6:+101386950 MsaT033883.1:CDS
ACAGCTCGAGCCTCGTGTCC+AGG 0.444598 6:+101388064 MsaT033883.1:CDS
TCCAGAAGATAGTGGTCCCT+TGG 0.454435 6:+101387977 MsaT033883.1:CDS
AAAGGTGAAGCGTATAGAAA+TGG 0.455948 6:+101387854 MsaT033883.1:CDS
CTTAACATGGAAGGGCCATA+TGG 0.467460 6:+101387810 MsaT033883.1:CDS
GCTTTCCCAATCCTTTCCCC+TGG 0.468580 6:-101388082 None:intergenic
CCTCTTCTTCCAAAACAAAT+CGG 0.471830 6:-101388016 None:intergenic
AGGATTGGGAAAGCTTCTAA+TGG 0.474839 6:+101388091 MsaT033883.1:CDS
ACAAATATATAGCATGCTTC+AGG 0.475325 6:-101387882 None:intergenic
GGCGTTGTTGTGAGTTGCAT+TGG 0.483691 6:-101385332 None:intergenic
CTCTCAGTGTGTTTAACGTC+AGG 0.484898 6:+101386894 MsaT033883.1:CDS
CAGCTCGAGCCTCGTGTCCA+GGG 0.491833 6:+101388065 MsaT033883.1:CDS
GAAGATAGTGGTCCCTTGGT+TGG 0.495248 6:+101387981 MsaT033883.1:CDS
TCTTGCCACTCAGATCCATA+TGG 0.513626 6:-101387825 None:intergenic
TATATATTGTTTAATAGGAG+AGG 0.517646 6:-101386934 None:intergenic
TCGTGTCCAGGGGAAAGGAT+TGG 0.525244 6:+101388076 MsaT033883.1:CDS
TGAGTGGCAAGAGGAAGAAA+AGG 0.525897 6:+101387836 MsaT033883.1:CDS
ATCTACAGCTTAACATGGAA+GGG 0.529467 6:+101387802 MsaT033883.1:intron
TCTTCTGGAAGACTAGTTGA+AGG 0.533222 6:-101387963 None:intergenic
GAGCCTCGTGTCCAGGGGAA+AGG 0.535185 6:+101388071 MsaT033883.1:CDS
ATGCTATATATTTGTGCATG+AGG 0.542195 6:+101387890 MsaT033883.1:CDS
AATCTACAGCTTAACATGGA+AGG 0.544882 6:+101387801 MsaT033883.1:intron
ACCTTCTAAAGTTTATCCAA+TGG 0.547249 6:+101389197 MsaT033883.1:CDS
TGTCGTGCTAACTATTCAAA+AGG 0.560643 6:+101388406 MsaT033883.1:CDS
TGTGAGTTGCATTGGTTCCA+CGG 0.567819 6:-101385324 None:intergenic
ACTAGTCTTCCAGAAGATAG+TGG 0.569548 6:+101387969 MsaT033883.1:CDS
TGTAAAACCAGCAATGAACT+CGG 0.592834 6:+101385285 None:intergenic
AATCCTTTCCCCTGGACACG+AGG 0.597062 6:-101388074 None:intergenic
CATTGGTTCCACGGTCACGT+TGG 0.600954 6:-101385315 None:intergenic
GTGTGTTTAACGTCAGGACA+TGG 0.610060 6:+101386900 MsaT033883.1:CDS
AGCTCGAGCCTCGTGTCCAG+GGG 0.623716 6:+101388066 MsaT033883.1:CDS
AGTTCATACACATAAAGTAC+CGG 0.630714 6:-101388041 None:intergenic
CAATCTAATGGTGCACCGAG+TGG 0.640629 6:-101389361 None:intergenic
GAGACGCTCCAACGTGACCG+TGG 0.649093 6:+101385307 MsaT033883.1:CDS
ATATGGATCTGAGTGGCAAG+AGG 0.653218 6:+101387827 MsaT033883.1:CDS
TCTAAATCTACAGCTTAACA+TGG 0.653637 6:+101387797 MsaT033883.1:intron
TAATGTACAAATCCAACCAA+GGG 0.659606 6:-101387993 None:intergenic
ACGCCGTCGTAACTGAGAGA+AGG 0.663934 6:+101385350 MsaT033883.1:CDS
GTAATGTACAAATCCAACCA+AGG 0.665929 6:-101387994 None:intergenic
GACGACAACAGTGTTGACTG+AGG 0.677893 6:+101387935 MsaT033883.1:CDS
TAACCTTCTCTCAGTTACGA+CGG 0.694983 6:-101385353 None:intergenic
ATAAACTTTAGAAGGTGACG+TGG 0.706293 6:-101389190 None:intergenic
GATAAACGGGAAAAGAAGCG+AGG 0.710792 6:-101385527 None:intergenic
AAGGGCCATATGGATCTGAG+TGG 0.737924 6:+101387820 MsaT033883.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 101385298 101389379 101385298 ID=MsaG033883
Chr6 mRNA 101385298 101389379 101385298 ID=MsaT033883.1;Parent=MsaG033883
Chr6 exon 101385298 101385387 101385298 ID=MsaT033883.1.exon1;Parent=MsaT033883.1
Chr6 CDS 101385298 101385387 101385298 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101385465 101385567 101385465 ID=MsaT033883.1.exon2;Parent=MsaT033883.1
Chr6 CDS 101385465 101385567 101385465 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101386892 101386971 101386892 ID=MsaT033883.1.exon3;Parent=MsaT033883.1
Chr6 CDS 101386892 101386971 101386892 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101387810 101388139 101387810 ID=MsaT033883.1.exon4;Parent=MsaT033883.1
Chr6 CDS 101387810 101388139 101387810 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101388392 101388468 101388392 ID=MsaT033883.1.exon5;Parent=MsaT033883.1
Chr6 CDS 101388392 101388468 101388392 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101389176 101389218 101389176 ID=MsaT033883.1.exon6;Parent=MsaT033883.1
Chr6 CDS 101389176 101389218 101389176 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Chr6 exon 101389293 101389379 101389293 ID=MsaT033883.1.exon7;Parent=MsaT033883.1
Chr6 CDS 101389293 101389379 101389293 ID=cds.MsaT033883.1;Parent=MsaT033883.1
Gene Sequence

>MsaT033883.1

ATGAACTCGGAGACGCTCCAACGTGACCGTGGAACCAATGCAACTCACAACAACGCCGTCGTAACTGAGAGAAGGTTAACACGCAGCGAACTTCTCGCAAAGAAGAACGCAAATGAACAACTCATCAAAGCAGCATATAGCATAAAGGATCACCTCGCTTCTTTTCCCGTTTATCGCTGCTTCGAAATCAACAGTCTCTCAGTGTGTTTAACGTCAGGACATGGCACTAAACTTTCCTCTCCTATTAAACAATATATACAAAGACTTCTTAAGCTTAACATGGAAGGGCCATATGGATCTGAGTGGCAAGAGGAAGAAAAGGTGAAGCGTATAGAAATGGTTGATCCTGAAGCATGCTATATATTTGTGCATGAGGTTACTAATGCAAATGCTGACGAGACGACAACAGTGTTGACTGAGGAAGATCCTTCAACTAGTCTTCCAGAAGATAGTGGTCCCTTGGTTGGATTTGTACATTACCGATTTGTTTTGGAAGAAGAGGTACCGGTACTTTATGTGTATGAACTACAGCTCGAGCCTCGTGTCCAGGGGAAAGGATTGGGAAAGCTTCTAATGGAACTTATTGAGCTTATCGCCCAAAATAACTGCATGGGTGCTGTCGTGCTAACTATTCAAAAGGCGAACATATCAGCTATGAATTTTTACACTAGTAAACTAAGATATATCATATCAGCCACGTCACCTTCTAAAGTTTATCCAATGATGGAGAAGAGTTATGAAATTCTTTGCAGAATATTTAATGATGAAGCTAAAACTATTTTTGAGGTAAACCACTCGGTGCACCATTAG

Protein sequence

>MsaT033883.1

MNSETLQRDRGTNATHNNAVVTERRLTRSELLAKKNANEQLIKAAYSIKDHLASFPVYRCFEINSLSVCLTSGHGTKLSSPIKQYIQRLLKLNMEGPYGSEWQEEEKVKRIEMVDPEACYIFVHEVTNANADETTTVLTEEDPSTSLPEDSGPLVGFVHYRFVLEEEVPVLYVYELQLEPRVQGKGLGKLLMELIELIAQNNCMGAVVLTIQKANISAMNFYTSKLRYIISATSPSKVYPMMEKSYEILCRIFNDEAKTIFEVNHSVHH*