Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033898 | XP_024642081.1 | 82.528 | 269 | 27 | 3 | 1 | 255 | 1 | 263 | 4.47e-156 | 444 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033898 | sp|Q568K5|NAA40_DANRE | 35.111 | 225 | 110 | 6 | 18 | 237 | 23 | 216 | 5.28e-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033898 | tr|A0A396HG85|A0A396HG85_MEDTR | 82.528 | 269 | 27 | 3 | 1 | 255 | 1 | 263 | 2.13e-156 | 444 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG033898 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG002532 | MsaG033898 | 0.801536 | 1.440869e-48 | 2.016948e-46 |
| MsaG004272 | MsaG033898 | 0.844359 | 1.485346e-58 | 6.664041e-56 |
| MsaG008874 | MsaG033898 | 0.802319 | 9.968462e-49 | 1.420554e-46 |
| MsaG016459 | MsaG033898 | 0.812337 | 7.664830e-51 | 1.386081e-48 |
| MsaG019803 | MsaG033898 | 0.806611 | 1.282390e-49 | 2.019182e-47 |
| MsaG028188 | MsaG033898 | 0.815634 | 1.448168e-51 | 2.844813e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033898 | MtrunA17_Chr6g0479551 | 82.528 | 269 | 27 | 3 | 1 | 255 | 1 | 263 | 4.11e-160 | 444 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG033898 | AT1G18335.3 | 51.230 | 244 | 108 | 3 | 10 | 249 | 26 | 262 | 2.93e-75 | 230 |
| MsaG033898 | AT1G18335.2 | 51.230 | 244 | 108 | 3 | 10 | 249 | 26 | 262 | 2.93e-75 | 230 |
| MsaG033898 | AT1G18335.1 | 51.029 | 243 | 108 | 3 | 10 | 248 | 26 | 261 | 6.26e-75 | 229 |
Find 51 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTATATATTGTTTAAT+AGG | 0.239001 | 6:-101905896 | None:intergenic |
| CCTCTTCTTCCAAAACAAAT+TGG | 0.253984 | 6:-101907114 | None:intergenic |
| ACCATTGGATAAACTTTAGA+AGG | 0.346375 | 6:-101908292 | None:intergenic |
| TGTACATTACCAATTTGTTT+TGG | 0.350136 | 6:+101907105 | MsaT033898.1:CDS |
| ATTTCGAAGCAGCGATAAAC+AGG | 0.379110 | 6:-101904737 | None:intergenic |
| TATCGCTGCTTCGAAATTAA+CGG | 0.393702 | 6:+101904743 | MsaT033898.1:CDS |
| CAAAGCAGCTTATAGCATAA+AGG | 0.405600 | 6:+101904697 | MsaT033898.1:CDS |
| GGCGTTGTTGTTTCTTGCAT+TGG | 0.412066 | 6:-101904536 | None:intergenic |
| CGTGTCCAGGGGAAAGGATT+GGG | 0.426522 | 6:+101907175 | MsaT033898.1:CDS |
| ACCAAGGGACCACTATCTTC+TGG | 0.440378 | 6:-101907076 | None:intergenic |
| ACAGCTCGAGCCTCGTGTCC+AGG | 0.444598 | 6:+101907162 | MsaT033898.1:CDS |
| ACAAATATATAGCGTGCTTC+AGG | 0.453405 | 6:-101906980 | None:intergenic |
| TCCAGAAGATAGTGGTCCCT+TGG | 0.454435 | 6:+101907075 | MsaT033898.1:CDS |
| AAAGGTGAAGCGTATAGAAA+TGG | 0.455948 | 6:+101906952 | MsaT033898.1:CDS |
| TGCTTTGATGAGTTGTTCAT+TGG | 0.464316 | 6:-101904681 | None:intergenic |
| CTTAACATGGAAGGGCCATA+TGG | 0.467460 | 6:+101906908 | MsaT033898.1:CDS |
| GCTTTCCCAATCCTTTCCCC+TGG | 0.468580 | 6:-101907180 | None:intergenic |
| AGGATTGGGAAAGCTTCTAA+TGG | 0.474839 | 6:+101907189 | MsaT033898.1:CDS |
| TATTGAGCTTATAGCCCAAA+AGG | 0.479862 | 6:+101907216 | MsaT033898.1:CDS |
| CTCTCAGTGTGTTTAACGTC+AGG | 0.484898 | 6:+101905851 | MsaT033898.1:CDS |
| CATTGGTTCCACGGTGATGT+TGG | 0.489688 | 6:-101904519 | None:intergenic |
| CAGCTCGAGCCTCGTGTCCA+GGG | 0.491833 | 6:+101907163 | MsaT033898.1:CDS |
| GAAGATAGTGGTCCCTTGGT+TGG | 0.495248 | 6:+101907079 | MsaT033898.1:CDS |
| TCTTGCCACTCAGATCCATA+TGG | 0.513626 | 6:-101906923 | None:intergenic |
| TATATATTGTTTAATAGGAG+AGG | 0.517646 | 6:-101905891 | None:intergenic |
| TCGTGTCCAGGGGAAAGGAT+TGG | 0.525244 | 6:+101907174 | MsaT033898.1:CDS |
| TGAGTGGCAAGAGGAAGAAA+AGG | 0.525897 | 6:+101906934 | MsaT033898.1:CDS |
| ATCTACAGCTTAACATGGAA+GGG | 0.529467 | 6:+101906900 | MsaT033898.1:intron |
| TGTTTCTTGCATTGGTTCCA+CGG | 0.532357 | 6:-101904528 | None:intergenic |
| TCTTCTGGAAGACTAGTTGA+AGG | 0.533222 | 6:-101907061 | None:intergenic |
| GAGCCTCGTGTCCAGGGGAA+AGG | 0.535185 | 6:+101907169 | MsaT033898.1:CDS |
| AATCTACAGCTTAACATGGA+AGG | 0.544882 | 6:+101906899 | MsaT033898.1:intron |
| ACCTTCTAAAGTTTATCCAA+TGG | 0.559757 | 6:+101908291 | MsaT033898.1:CDS |
| TGTCGTGCTAACTATTCAAA+AGG | 0.560643 | 6:+101907504 | MsaT033898.1:CDS |
| CAATCTAATGGTGCAGTGAG+TGG | 0.567174 | 6:-101908456 | None:intergenic |
| ACTAGTCTTCCAGAAGATAG+TGG | 0.569548 | 6:+101907067 | MsaT033898.1:CDS |
| ACGCTATATATTTGTGCATG+AGG | 0.569652 | 6:+101906988 | MsaT033898.1:CDS |
| AATCCTTTCCCCTGGACACG+AGG | 0.597062 | 6:-101907172 | None:intergenic |
| ACGGTGATGTTGGAGCATCT+CGG | 0.600011 | 6:-101904509 | None:intergenic |
| GTGTGTTTAACGTCAGGACA+TGG | 0.610060 | 6:+101905857 | MsaT033898.1:CDS |
| AGCTCGAGCCTCGTGTCCAG+GGG | 0.623716 | 6:+101907164 | MsaT033898.1:CDS |
| AGTTCATACACATAAAGTAC+CGG | 0.630714 | 6:-101907139 | None:intergenic |
| GTAATGTACAAATCCAACCA+AGG | 0.648535 | 6:-101907092 | None:intergenic |
| ATATGGATCTGAGTGGCAAG+AGG | 0.653218 | 6:+101906925 | MsaT033898.1:CDS |
| TCTAAATCTACAGCTTAACA+TGG | 0.653637 | 6:+101906895 | MsaT033898.1:intron |
| ATAAACTTTAGAAGGTGATG+TGG | 0.664725 | 6:-101908284 | None:intergenic |
| TAATGTACAAATCCAACCAA+GGG | 0.675619 | 6:-101907091 | None:intergenic |
| GACGACAACAGTGTTGACTG+AGG | 0.677893 | 6:+101907033 | MsaT033898.1:CDS |
| GATAAACAGGAAAAGCAGCG+AGG | 0.735199 | 6:-101904724 | None:intergenic |
| AAGGGCCATATGGATCTGAG+TGG | 0.737924 | 6:+101906918 | MsaT033898.1:CDS |
| GAGATGCTCCAACATCACCG+TGG | 0.782648 | 6:+101904511 | MsaT033898.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 101904502 | 101908474 | 101904502 | ID=MsaG033898 |
| Chr6 | mRNA | 101904502 | 101908474 | 101904502 | ID=MsaT033898.1;Parent=MsaG033898 |
| Chr6 | exon | 101904502 | 101904558 | 101904502 | ID=MsaT033898.1.exon1;Parent=MsaT033898.1 |
| Chr6 | CDS | 101904502 | 101904558 | 101904502 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101904662 | 101904764 | 101904662 | ID=MsaT033898.1.exon2;Parent=MsaT033898.1 |
| Chr6 | CDS | 101904662 | 101904764 | 101904662 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101905849 | 101905919 | 101905849 | ID=MsaT033898.1.exon3;Parent=MsaT033898.1 |
| Chr6 | CDS | 101905849 | 101905919 | 101905849 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101906908 | 101907237 | 101906908 | ID=MsaT033898.1.exon4;Parent=MsaT033898.1 |
| Chr6 | CDS | 101906908 | 101907237 | 101906908 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101907490 | 101907566 | 101907490 | ID=MsaT033898.1.exon5;Parent=MsaT033898.1 |
| Chr6 | CDS | 101907490 | 101907566 | 101907490 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101908270 | 101908312 | 101908270 | ID=MsaT033898.1.exon6;Parent=MsaT033898.1 |
| Chr6 | CDS | 101908270 | 101908312 | 101908270 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Chr6 | exon | 101908388 | 101908474 | 101908388 | ID=MsaT033898.1.exon7;Parent=MsaT033898.1 |
| Chr6 | CDS | 101908388 | 101908474 | 101908388 | ID=cds.MsaT033898.1;Parent=MsaT033898.1 |
| Gene Sequence |
| Protein sequence |
