AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033943

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033943 MtrunA17_Chr1g0203051 61.006 159 60 1 1 157 64 222 2.37e-66 213
MsaG033943 MtrunA17_Chr8g0389811 45.669 127 67 1 1 125 69 195 1.49e-38 130
MsaG033943 MtrunA17_Chr1g0171741 37.063 143 89 1 3 144 120 262 2.97e-28 105
MsaG033943 MtrunA17_Chr2g0331371 37.903 124 75 1 1 122 40 163 2.09e-25 96.7
MsaG033943 MtrunA17_Chr2g0287651 32.895 152 100 1 7 156 66 217 1.77e-23 95.9
MsaG033943 MtrunA17_Chr6g0473421 40.659 91 52 1 1 89 40 130 2.12e-22 87.4
MsaG033943 MtrunA17_Chr1g0172931 42.857 98 54 1 1 96 61 158 1.22e-19 80.5
MsaG033943 MtrunA17_Chr2g0290391 33.333 129 83 2 28 153 63 191 7.32e-17 77.0
MsaG033943 MtrunA17_Chr1g0198761 35.766 137 76 6 28 153 59 194 5.83e-16 74.3
MsaG033943 MtrunA17_Chr6g0458391 31.757 148 96 4 5 149 80 225 1.96e-15 72.8
MsaG033943 MtrunA17_Chr6g0458391 34.259 108 67 4 28 133 237 342 3.84e-11 60.5
MsaG033943 MtrunA17_Chr2g0276841 31.061 132 84 4 28 153 82 212 9.65e-15 70.9
MsaG033943 MtrunA17_Chr5g0431001 29.577 142 90 3 22 153 67 208 2.82e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033943 AT5G28530.4 33.846 130 81 3 28 153 67 195 1.67e-20 87.8
MsaG033943 AT5G28530.5 33.846 130 81 3 28 153 67 195 1.87e-20 87.4
MsaG033943 AT5G28530.1 33.846 130 81 3 28 153 67 195 2.15e-20 87.4
MsaG033943 AT5G28530.3 33.846 130 81 3 28 153 67 195 2.23e-20 87.4
MsaG033943 AT5G28530.2 33.846 130 81 3 28 153 67 195 2.23e-20 87.4
MsaG033943 AT1G10240.2 30.303 132 85 4 28 153 60 190 1.11e-14 70.9
MsaG033943 AT1G10240.1 30.303 132 85 4 28 153 60 190 1.11e-14 70.9

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTAAGCGATCCTGAAAA+TGG 0.320523 6:+102961844 None:intergenic
CTCATACTTCCCATCCAAAA+AGG 0.333792 6:+102961557 None:intergenic
ATTCCTACGTCACGCATCTA+TGG 0.380981 6:-102961462 MsaT033943.1:CDS
TTTGTCATAGAGAAGGTTAC+AGG 0.397074 6:-102961719 MsaT033943.1:CDS
AAAGCCGTGTTGTTAAGAAC+AGG 0.447436 6:-102961770 MsaT033943.1:CDS
GTACACGTTGACTTGGTAAT+GGG 0.464517 6:-102961621 MsaT033943.1:CDS
AGTACACGTTGACTTGGTAA+TGG 0.469899 6:-102961622 MsaT033943.1:CDS
GGGTCGATGGTACATAAAAT+CGG 0.475635 6:-102961601 MsaT033943.1:CDS
AACTAGTAGGTGTGGTTGCA+TGG 0.492607 6:-102961655 MsaT033943.1:CDS
ATGAAAATGATGAGGAAAGC+TGG 0.493711 6:-102961486 MsaT033943.1:CDS
GAGAGCAGAAGCCAACTAGT+AGG 0.503280 6:-102961668 MsaT033943.1:CDS
TCGCTCATCTTCCTATGTGC+AGG 0.519995 6:+102961525 None:intergenic
CCTTAAAATTAATTCTGTCA+AGG 0.525060 6:+102961895 None:intergenic
TAGGGTCGGATGAAATCATA+AGG 0.528599 6:-102961869 MsaT033943.1:CDS
AATTCAAGTACACGTTGACT+TGG 0.536738 6:-102961628 MsaT033943.1:CDS
ACCTTTGTTTGTCATAGAGA+AGG 0.542041 6:-102961726 MsaT033943.1:CDS
ATGCAACCACACCTACTAGT+TGG 0.551707 6:+102961657 None:intergenic
AATTTGTACGGTTGTGTACG+TGG 0.562187 6:-102961807 MsaT033943.1:CDS
CATCCCTGTTCTTAACAACA+CGG 0.581390 6:+102961766 None:intergenic
CAGAAGCCAACTAGTAGGTG+TGG 0.587238 6:-102961663 MsaT033943.1:CDS
TTGAAAGTAATGCCGCCGAT+AGG 0.598358 6:-102961695 MsaT033943.1:CDS
TTGTCATAGAGAAGGTTACA+GGG 0.601328 6:-102961718 MsaT033943.1:CDS
CGTGTTGTTAAGAACAGGGA+TGG 0.609459 6:-102961765 MsaT033943.1:CDS
ACCTTCTCTATGACAAACAA+AGG 0.615884 6:+102961725 None:intergenic
TTGACTTGGTAATGGGTCGA+TGG 0.615996 6:-102961614 MsaT033943.1:CDS
AGGGAATGCTACCTGCACAT+AGG 0.625271 6:-102961536 MsaT033943.1:CDS
AATACCAAATGAAAATGATG+AGG 0.636392 6:-102961494 MsaT033943.1:CDS
AAACCATAGATGCGTGACGT+AGG 0.642746 6:+102961459 None:intergenic
AAGCCGTGTTGTTAAGAACA+GGG 0.681295 6:-102961769 MsaT033943.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 102961431 102961925 102961431 ID=MsaG033943
Chr6 mRNA 102961431 102961925 102961431 ID=MsaT033943.1;Parent=MsaG033943
Chr6 exon 102961431 102961925 102961431 ID=MsaT033943.1.exon1;Parent=MsaT033943.1
Chr6 CDS 102961431 102961925 102961431 ID=cds.MsaT033943.1;Parent=MsaT033943.1
Gene Sequence

>MsaT033943.1

ATGGCTGACCTTGACAGAATTAATTTTAAGGAAATAGGGTCGGATGAAATCATAAGGTACCATTTTCAGGATCGCTTAGTTGCATTCAAGTTTTACAATTTGTACGGTTGTGTACGTGGATTTTCCGGTAGAAAAAGCCGTGTTGTTAAGAACAGGGATGGCAATGTTGTTCAACAAACCTTTGTTTGTCATAGAGAAGGTTACAGGGTTGAAAGTAATGCCGCCGATAGGAAAAGAGAGCAGAAGCCAACTAGTAGGTGTGGTTGCATGGCAAAAATTCAAGTACACGTTGACTTGGTAATGGGTCGATGGTACATAAAATCGGTTGACGATATTCACAATCACACCTTTTTGGATGGGAAGTATGAGGGAATGCTACCTGCACATAGGAAGATGAGCGATTATGACAAATACCAAATGAAAATGATGAGGAAAGCTGGCATTCCTACGTCACGCATCTATGGTTTTTTTTGCTTCACAGGCTGGTGGATTTAA
Protein sequence

>MsaT033943.1

MADLDRINFKEIGSDEIIRYHFQDRLVAFKFYNLYGCVRGFSGRKSRVVKNRDGNVVQQTFVCHREGYRVESNAADRKREQKPTSRCGCMAKIQVHVDLVMGRWYIKSVDDIHNHTFLDGKYEGMLPAHRKMSDYDKYQMKMMRKAGIPTSRIYGFFCFTGWWI*