AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG033948

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033948 MtrunA17_Chr6g0481041 97.805 729 13 3 1 727 1 728 0.0 1484
MsaG033948 MtrunA17_Chr7g0246631 49.721 718 322 12 24 726 85 778 0.0 679
MsaG033948 MtrunA17_Chr8g0350321 48.966 725 331 10 21 726 106 810 0.0 679
MsaG033948 MtrunA17_Chr4g0024351 47.784 722 334 13 19 727 43 734 0.0 662
MsaG033948 MtrunA17_Chr5g0410711 48.801 709 337 15 30 727 89 782 0.0 662
MsaG033948 MtrunA17_Chr2g0322731 48.540 719 330 14 12 723 81 766 0.0 655
MsaG033948 MtrunA17_Chr1g0203671 47.331 712 343 12 28 722 93 789 0.0 639
MsaG033948 MtrunA17_Chr7g0224451 64.000 500 112 6 19 510 3 442 0.0 634
MsaG033948 MtrunA17_Chr2g0331581 41.026 702 350 12 34 724 143 791 0.0 564
MsaG033948 MtrunA17_Chr6g0472471 42.458 716 362 15 34 722 73 765 0.0 535
MsaG033948 MtrunA17_Chr7g0224481 62.148 391 86 5 19 401 3 339 9.79e-162 470
MsaG033948 MtrunA17_Chr7g0224491 66.460 322 82 7 404 725 1 296 3.55e-135 400
MsaG033948 MtrunA17_Chr5g0394111 35.235 701 345 13 37 722 40 646 9.98e-135 412
MsaG033948 MtrunA17_Chr2g0292731 42.188 512 265 11 221 722 58 548 1.85e-126 387
MsaG033948 MtrunA17_Chr2g0292741 42.745 510 260 11 221 722 50 535 5.74e-126 385
MsaG033948 MtrunA17_Chr2g0292761 33.601 622 323 19 109 722 1 540 4.07e-100 318
MsaG033948 MtrunA17_Chr5g0419821 61.194 134 51 1 18 150 21 154 1.46e-51 177
MsaG033948 MtrunA17_Chr7g0224431 54.938 162 31 2 250 411 1 120 3.09e-47 163
MsaG033948 MtrunA17_Chr7g0224461 63.077 130 35 4 596 725 1 117 7.08e-37 134
MsaG033948 MtrunA17_Chr2g0319671 40.462 173 95 3 281 453 104 268 7.27e-33 129
MsaG033948 MtrunA17_Chr2g0319681 32.231 242 142 6 486 722 1 225 8.16e-32 124
MsaG033948 MtrunA17_Chr2g0319701 35.270 241 126 10 490 722 5 223 5.03e-29 116
MsaG033948 MtrunA17_Chr2g0304191 43.284 134 74 2 235 367 1 133 1.49e-28 111
MsaG033948 MtrunA17_Chr2g0319691 40.870 115 60 3 341 453 1 109 7.03e-21 90.1
MsaG033948 MtrunA17_Chr1g0205981 29.592 196 105 6 85 270 1 173 7.99e-14 70.9
MsaG033948 MtrunA17_Chr1g0163701 86.486 37 5 0 341 377 238 274 1.37e-13 73.6
MsaG033948 MtrunA17_Chr4g0028991 30.220 182 96 5 14 164 9 190 1.56e-13 74.3
MsaG033948 MtrunA17_Chr7g0265521 38.835 103 60 1 26 125 56 158 3.49e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG033948 AT1G73360.1 63.483 712 229 13 26 727 28 718 0.0 887
MsaG033948 AT1G17920.2 59.972 707 227 14 33 727 24 686 0.0 836
MsaG033948 AT1G17920.1 59.972 707 227 14 33 727 24 686 0.0 836
MsaG033948 AT4G04890.2 51.836 708 304 16 27 724 57 737 0.0 700
MsaG033948 AT4G04890.1 51.836 708 304 16 27 724 57 737 0.0 700
MsaG033948 AT4G21750.2 50.419 716 316 15 30 724 62 759 0.0 686
MsaG033948 AT4G21750.1 50.419 716 316 15 30 724 62 759 0.0 686
MsaG033948 AT4G21750.3 50.419 716 316 15 30 724 62 759 0.0 686
MsaG033948 AT4G21750.4 50.419 716 316 15 30 724 62 759 0.0 686
MsaG033948 AT1G05230.3 47.665 728 324 12 10 727 39 719 0.0 672
MsaG033948 AT1G05230.8 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.4 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.9 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.10 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.7 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.11 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.2 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.6 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.5 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT1G05230.1 47.534 730 324 13 10 727 39 721 0.0 667
MsaG033948 AT4G00730.1 47.692 715 326 12 22 726 126 802 0.0 638
MsaG033948 AT3G61150.1 46.732 719 344 15 29 727 109 808 0.0 632
MsaG033948 AT5G46880.3 44.626 735 344 19 21 722 102 806 0.0 573
MsaG033948 AT5G46880.1 44.626 735 344 19 21 722 102 806 0.0 573
MsaG033948 AT5G46880.2 44.626 735 344 19 21 722 102 806 0.0 573
MsaG033948 AT1G05230.12 49.556 563 254 10 30 587 64 601 0.0 548
MsaG033948 AT5G52170.3 43.266 698 315 15 34 726 61 682 0.0 540
MsaG033948 AT5G52170.1 43.266 698 315 15 34 726 61 682 0.0 540
MsaG033948 AT5G52170.2 42.939 701 318 15 34 726 61 687 0.0 537
MsaG033948 AT3G03260.1 40.878 729 357 16 24 725 16 697 3.02e-179 528
MsaG033948 AT1G79840.3 39.093 706 357 18 28 722 41 684 3.36e-172 510
MsaG033948 AT1G79840.6 39.093 706 357 18 28 722 41 684 3.36e-172 510
MsaG033948 AT1G79840.1 39.093 706 357 18 28 722 99 742 2.16e-171 510
MsaG033948 AT1G79840.2 39.093 706 357 18 28 722 128 771 1.87e-170 509
MsaG033948 AT2G32370.1 39.448 725 370 18 19 727 54 725 5.70e-164 490
MsaG033948 AT5G17320.1 40.136 735 373 19 22 724 18 717 8.28e-162 484
MsaG033948 AT4G17710.1 39.660 706 323 18 30 722 88 703 1.10e-158 476
MsaG033948 AT1G34650.1 39.757 742 361 17 22 724 13 707 3.24e-154 464
MsaG033948 AT1G79840.4 40.767 574 281 17 28 589 99 625 2.54e-143 434
MsaG033948 AT4G00730.2 49.554 448 197 9 22 462 126 551 2.16e-138 419
MsaG033948 AT3G03260.2 41.651 545 285 11 24 557 16 538 9.54e-137 414
MsaG033948 AT4G25530.1 34.807 701 393 16 30 723 40 683 1.07e-127 395
MsaG033948 AT1G79840.5 41.138 457 212 14 28 473 99 509 4.59e-105 331
MsaG033948 AT5G07260.1 24.910 554 333 19 97 642 11 489 1.28e-39 154
MsaG033948 AT5G07260.2 24.729 554 329 20 97 642 11 484 3.76e-39 152
MsaG033948 AT4G26920.1 23.239 426 242 14 235 652 72 420 9.39e-19 90.5
MsaG033948 AT5G60690.1 37.121 132 56 5 19 123 7 138 5.82e-14 76.3
MsaG033948 AT1G30490.1 34.821 112 65 2 16 119 4 115 2.06e-11 68.2
MsaG033948 AT1G52150.3 21.429 462 294 17 314 723 228 672 4.26e-11 67.0
MsaG033948 AT1G52150.1 21.429 462 294 17 314 723 228 672 5.90e-11 66.6
MsaG033948 AT1G52150.2 20.996 462 297 16 314 723 228 673 8.10e-11 66.2

Find 158 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTGGTTGAAATGTTTA+TGG 0.134213 6:+103166564 MsaT033948.1:CDS
GACTTATCAATGGGAAATTT+TGG 0.144806 6:+103166211 MsaT033948.1:CDS
CGCAAATTGAGCAAGGTTTA+TGG 0.148416 6:+103166999 MsaT033948.1:CDS
AACAGTTGCATTCCAAATTA+TGG 0.179679 6:+103168806 MsaT033948.1:CDS
CTCCTAGACAGATCAAATTT+TGG 0.209657 6:+103164919 MsaT033948.1:CDS
GTAAACTACTAACCATAATT+TGG 0.226737 6:-103168818 None:intergenic
ACTATTGAAGTTATCTCTTC+TGG 0.240170 6:+103166727 MsaT033948.1:CDS
TTGCAATATGAGCAACTTCT+TGG 0.244055 6:-103168088 None:intergenic
TAGTGGGATGTTCTTTCTAA+TGG 0.252600 6:+103168056 MsaT033948.1:intron
GAGTCTGTTGCAACTGTTAA+TGG 0.255130 6:+103168861 MsaT033948.1:CDS
TTCAACCGATTATTAGTCTT+AGG 0.265573 6:-103166481 None:intergenic
GCAACTGTTAATGGACTTAT+TGG 0.266967 6:+103168870 MsaT033948.1:CDS
TTCATAGATGTTAATTCAAT+TGG 0.304841 6:-103168600 None:intergenic
GGTCACTTGGATTGAACATT+TGG 0.308899 6:+103167221 MsaT033948.1:intron
TGAAAGGCTTGCTTGTTCTA+TGG 0.312367 6:+103167347 MsaT033948.1:CDS
ATCTATAGTGGAGCTGCATT+TGG 0.334195 6:+103167288 MsaT033948.1:CDS
CACATTCCAGTAACACCTTC+AGG 0.343396 6:+103168651 MsaT033948.1:CDS
GCAACTCCATCTCTGTGCTT+AGG 0.352073 6:+103168126 MsaT033948.1:CDS
GATTGGTCTTCCAATGTATT+TGG 0.360707 6:-103166146 None:intergenic
AGTGGGATGTTCTTTCTAAT+GGG 0.366936 6:+103168057 MsaT033948.1:intron
ATGTGAGAATATAGCTATAA+GGG 0.368026 6:+103165446 MsaT033948.1:CDS
TGCATAGTTGATGAACTTCC+AGG 0.370504 6:-103166280 None:intergenic
GTGAAACCTGAAGGTGTTAC+TGG 0.378283 6:-103168657 None:intergenic
GCAAATTGAGCAAGGTTTAT+GGG 0.381720 6:+103167000 MsaT033948.1:CDS
TTCATCATTGGACTTATCAA+TGG 0.382138 6:+103166201 MsaT033948.1:CDS
ATCGAAATTGAGGAGCAAAT+TGG 0.385407 6:-103167060 None:intergenic
CTTGATCTTGATCTTCTACC+TGG 0.386174 6:+103166262 MsaT033948.1:CDS
AAGAAATTGAAAACAGTTTG+TGG 0.386401 6:-103167910 None:intergenic
TGCAACCTTTGTTTCTCATC+AGG 0.387813 6:-103164873 None:intergenic
GAAGCTTCAATGCGAATATT+CGG 0.387981 6:-103166508 None:intergenic
ACCAATTCACATTTCATCAT+TGG 0.388149 6:+103166189 MsaT033948.1:CDS
CGATGCCATCGTCTTCCATC+TGG 0.388366 6:+103167079 MsaT033948.1:CDS
AGCTCAAACCTAGGGAATAA+TGG 0.400178 6:+103168762 MsaT033948.1:CDS
GAGAATCAGAAGAACCTTCA+TGG 0.402346 6:-103164625 None:intergenic
GATGTGAGAATATAGCTATA+AGG 0.404730 6:+103165445 MsaT033948.1:CDS
CAACTCCATCTCTGTGCTTA+GGG 0.406270 6:+103168127 MsaT033948.1:CDS
TTTGGTGGGCATGGAATGGT+TGG 0.409505 6:+103166229 MsaT033948.1:CDS
ATTACCTTGGAGTAGCCATT+AGG 0.412515 6:-103167121 None:intergenic
TCCTAGTGGTTCTATGCAAC+TGG 0.413430 6:+103166762 MsaT033948.1:CDS
TCTCGTGTTGTTATCATGAA+TGG 0.413589 6:+103166538 MsaT033948.1:CDS
TATAGAAATGCTATCTATAG+TGG 0.415183 6:+103167276 MsaT033948.1:CDS
GAAAGCTGTGTTGATTCATC+AGG 0.415581 6:+103168558 MsaT033948.1:CDS
GATAACAATAATAATCTTCA+AGG 0.418346 6:+103168705 MsaT033948.1:CDS
TTTCTATAAAGCCTATGAAC+TGG 0.420383 6:-103167261 None:intergenic
ATGCAATGGAAGAGATGATT+AGG 0.426158 6:+103166371 MsaT033948.1:CDS
GGTGGGCATGGAATGGTTGG+AGG 0.429152 6:+103166232 MsaT033948.1:CDS
GTGCATCAGCTAGTCATAGA+TGG 0.431673 6:+103167770 MsaT033948.1:CDS
CTGCTCTTTCCCTCAGGTGA+TGG 0.432769 6:-103167697 None:intergenic
AAGAAACAATCATCATTGAT+TGG 0.440524 6:-103165510 None:intergenic
GAAGTTGCTCATATTGCAAA+TGG 0.443252 6:+103168092 MsaT033948.1:CDS
AGGGAGTCAGAGTTACTGTT+AGG 0.446651 6:+103167815 MsaT033948.1:CDS
GGTTTCACAATCACTTCTGA+TGG 0.448901 6:+103168672 MsaT033948.1:CDS
AGTTATCTCTTCTGGAATGA+TGG 0.450155 6:+103166735 MsaT033948.1:CDS
ATCATGCTGCTCTTTCCCTC+AGG 0.452513 6:-103167703 None:intergenic
TCCAATGATGAAATGTGAAT+TGG 0.452865 6:-103166190 None:intergenic
TATCATGAATGGCTTAGCTT+TGG 0.453058 6:+103166549 MsaT033948.1:CDS
TCAACCGATTATTAGTCTTA+GGG 0.457224 6:-103166480 None:intergenic
GAAAGACAAGCTGGTTGGTA+AGG 0.465607 6:-103166310 None:intergenic
ACTGGTGGAAGCTGTGTGAT+TGG 0.466182 6:-103166163 None:intergenic
CACACTTTCAGCTTTGAATG+AGG 0.466242 6:+103167795 MsaT033948.1:CDS
AAAACAGTTTGTGGAGGGAG+AGG 0.466739 6:-103167901 None:intergenic
AAGAAGTAGAATTCTCTGAT+AGG 0.466986 6:-103166965 None:intergenic
TTCATTCAAGACATGCCTAA+TGG 0.467119 6:+103167106 MsaT033948.1:CDS
TCATCATTGGACTTATCAAT+GGG 0.471502 6:+103166202 MsaT033948.1:CDS
AATAATAATCTTCAAGGAAG+TGG 0.472661 6:+103168711 MsaT033948.1:CDS
CACTACCACCATTATTCCCT+AGG 0.473826 6:-103168770 None:intergenic
CTAGGGAATAATGGTGGTAG+TGG 0.488409 6:+103168771 MsaT033948.1:CDS
TATGTGCAGCTACTACCATT+TGG 0.488886 6:+103167875 MsaT033948.1:CDS
GTTAAGCAGAGAATTAGCTT+TGG 0.491594 6:+103164896 MsaT033948.1:CDS
GTTTCAGCCACCCGTGATCT+CGG 0.495016 6:+103167369 MsaT033948.1:CDS
GCATCAACAAGCTCAAACCT+AGG 0.505348 6:+103168753 MsaT033948.1:CDS
CTTTGTTTCTCATCAGGATG+AGG 0.506519 6:-103164867 None:intergenic
TCAATTTCTTCAAAGATGAA+AGG 0.508178 6:+103167923 MsaT033948.1:CDS
CTGCATTTGGAGCTGAAAGA+TGG 0.510050 6:+103167301 MsaT033948.1:CDS
GAAATGTGAATTGGTGCAAC+TGG 0.511399 6:-103166181 None:intergenic
AGTGGAGCAAGTGGGTCCCC+TGG 0.514639 6:+103164578 MsaT033948.1:CDS
TGTTCCCTAAGACTAATAAT+CGG 0.516268 6:+103166476 MsaT033948.1:CDS
CTTTCCTACCATTGTGACAA+TGG 0.517329 6:+103166699 MsaT033948.1:CDS
AGACGATGGCATCGAAATTG+AGG 0.521276 6:-103167070 None:intergenic
AGCACAGAGATGGAGTTGCC+AGG 0.521625 6:-103168122 None:intergenic
GCCACCCGTGATCTCGGTGG+AGG 0.523884 6:+103167375 MsaT033948.1:CDS
TATTCGCATTGAAGCTTCGA+GGG 0.530981 6:+103166513 MsaT033948.1:CDS
TCCATGTCAGAAAGACAAGC+TGG 0.531398 6:-103166319 None:intergenic
CTTGCCATTGTCACAATGGT+AGG 0.533442 6:-103166703 None:intergenic
CTCTTCAGAGAATGTGTGAA+AGG 0.534959 6:+103167331 MsaT033948.1:CDS
GAGCTGATAACTGTCAACTA+AGG 0.535625 6:+103165403 MsaT033948.1:CDS
GTTATCTCTTCTGGAATGAT+GGG 0.546482 6:+103166736 MsaT033948.1:CDS
GGGAATAATGGTGGTAGTGG+AGG 0.551862 6:+103168774 MsaT033948.1:CDS
GAACAGAAGGACTCAGATGA+AGG 0.553957 6:+103164947 MsaT033948.1:CDS
AGCATTGCTGCCAAATACAT+TGG 0.554442 6:+103166136 MsaT033948.1:CDS
ATATACCTCCACCGAGATCA+CGG 0.554654 6:-103167380 None:intergenic
GTTGTCATGAGGAAAATCAT+AGG 0.556836 6:-103167038 None:intergenic
ACACTTTCAGCTTTGAATGA+GGG 0.558221 6:+103167796 MsaT033948.1:CDS
AATTGAAAACAGTTTGTGGA+GGG 0.561046 6:-103167906 None:intergenic
TACTGTACGCAAATTGAGCA+AGG 0.562069 6:+103166992 MsaT033948.1:CDS
GCACAGAGATGGAGTTGCCA+GGG 0.562213 6:-103168121 None:intergenic
AGTGGTAATGTTAATGCTGA+TGG 0.563501 6:+103168729 MsaT033948.1:CDS
AGATGAAAGGAAAAGATCAC+AGG 0.565819 6:+103167936 MsaT033948.1:CDS
ATCTCTTCTGGAATGATGGG+TGG 0.566526 6:+103166739 MsaT033948.1:CDS
GTGATCTGCCCCTCTTGTGG+TGG 0.567379 6:+103165483 MsaT033948.1:CDS
ACCAGCTTGTCTTTCTGACA+TGG 0.569306 6:+103166318 MsaT033948.1:CDS
ACCATCTGCTAAACTTAACA+TGG 0.570228 6:+103168839 MsaT033948.1:CDS
CATCATCATCATCACCATGA+AGG 0.573382 6:+103164611 MsaT033948.1:CDS
GTTAGGAAGAGTACTGAACT+TGG 0.574526 6:+103167832 MsaT033948.1:CDS
GCACATAGCACAACACCACT+AGG 0.575221 6:-103167859 None:intergenic
TTAATCTTGAAAACTATGAG+AGG 0.580418 6:+103166452 MsaT033948.1:CDS
TCAGCGATTCCATCACCTGA+GGG 0.581584 6:+103167688 MsaT033948.1:intron
AAATGTGCAACTCAAAGAAG+AGG 0.585426 6:+103165557 MsaT033948.1:CDS
AACCAAAATTTGATCTGTCT+AGG 0.585787 6:-103164921 None:intergenic
TCTTGAATGAAGCAGCCAGA+TGG 0.586658 6:-103167094 None:intergenic
CTGAAGGTGTTACTGGAATG+TGG 0.586831 6:-103168650 None:intergenic
ATTGCAAATGGTTCTCACCC+TGG 0.587680 6:+103168104 MsaT033948.1:CDS
TCTTACCCTAAGCACAGAGA+TGG 0.590044 6:-103168132 None:intergenic
TCCATGTTAAGTTTAGCAGA+TGG 0.590444 6:-103168840 None:intergenic
CTCATTCAAAGCTGAAAGTG+TGG 0.591958 6:-103167794 None:intergenic
ATATTCGCATTGAAGCTTCG+AGG 0.592812 6:+103166512 MsaT033948.1:CDS
CTCATCCTGATGAGAAACAA+AGG 0.606148 6:+103164868 MsaT033948.1:CDS
TAGGAAAGAGAGCCATCCAC+TGG 0.606397 6:-103166684 None:intergenic
AGTCCTTGCCATTGTCACAA+TGG 0.607736 6:-103166707 None:intergenic
TGGAGGGAGAGGTAACCAAA+TGG 0.608618 6:-103167890 None:intergenic
GATTGGAGGACCACCACAAG+AGG 0.608794 6:-103165493 None:intergenic
AAACAATCATCATTGATTGG+AGG 0.609512 6:-103165507 None:intergenic
TATACCTCCACCGAGATCAC+GGG 0.611974 6:-103167379 None:intergenic
GAGTATCATAGTGGAGCAAG+TGG 0.613997 6:+103164569 MsaT033948.1:CDS
TGACATTGCATCAAATGCAA+TGG 0.614917 6:+103166357 MsaT033948.1:CDS
TGGATGAAATCAAACTCTGA+TGG 0.615224 6:+103166418 MsaT033948.1:CDS
ACACTGCTAATCAGATTCAA+AGG 0.615484 6:+103164675 MsaT033948.1:CDS
GATGAAGCTAGCACAAAGGA+TGG 0.618354 6:+103167723 MsaT033948.1:CDS
TTCAGCGATTCCATCACCTG+AGG 0.619756 6:+103167687 MsaT033948.1:intron
CATCAACAAGCTCAAACCTA+GGG 0.620380 6:+103168754 MsaT033948.1:CDS
AGAAGATCAAGATCAAGAGA+AGG 0.622073 6:-103166256 None:intergenic
CTACCATTGTGACAATGGCA+AGG 0.622296 6:+103166704 MsaT033948.1:CDS
GGAATGATGGGTGGTCCTAG+TGG 0.629709 6:+103166748 MsaT033948.1:CDS
AAATTGAAAACAGTTTGTGG+AGG 0.630363 6:-103167907 None:intergenic
AATTCTCTGATAGGAACAAG+TGG 0.634478 6:-103166956 None:intergenic
TGCAGACTAATGAGCCTCTG+TGG 0.639149 6:+103166398 MsaT033948.1:CDS
TGTCAGAAAGACAAGCTGGT+TGG 0.640801 6:-103166315 None:intergenic
ATGTGAATTGGTGCAACTGG+TGG 0.640938 6:-103166178 None:intergenic
GGAGCAAATTGGTTGTCATG+AGG 0.644218 6:-103167049 None:intergenic
ACCTCCACCGAGATCACGGG+TGG 0.644662 6:-103167376 None:intergenic
AATGTGATCTGCCCCTCTTG+TGG 0.645075 6:+103165480 MsaT033948.1:CDS
ATTGGAGGACCACCACAAGA+GGG 0.645788 6:-103165492 None:intergenic
CATGCCTAATGGCTACTCCA+AGG 0.648553 6:+103167117 MsaT033948.1:CDS
GAAGTGATTGTGAAACCTGA+AGG 0.654254 6:-103168666 None:intergenic
GAATCTGATTAGCAGTGTGA+CGG 0.657531 6:-103164670 None:intergenic
TCAGCCACCCGTGATCTCGG+TGG 0.670096 6:+103167372 MsaT033948.1:CDS
TGAAGGTGTTACTGGAATGT+GGG 0.675743 6:-103168649 None:intergenic
GCATGATGAAGCTAGCACAA+AGG 0.680662 6:+103167719 MsaT033948.1:CDS
ACTGAACTTGGACAGCCTAG+TGG 0.686089 6:+103167844 MsaT033948.1:CDS
TCAAACCTAGGGAATAATGG+TGG 0.686611 6:+103168765 MsaT033948.1:CDS
GAGTTTGATTTCATCCACAG+AGG 0.690229 6:-103166412 None:intergenic
TTGGAGGACCACCACAAGAG+GGG 0.698087 6:-103165491 None:intergenic
AGCAGCCAGATGGAAGACGA+TGG 0.710624 6:-103167084 None:intergenic
AGTATCATAGTGGAGCAAGT+GGG 0.711511 6:+103164570 MsaT033948.1:CDS
TCTGATTAGCAGTGTGACGG+TGG 0.712245 6:-103164667 None:intergenic
ACCAGTTGCATAGAACCACT+AGG 0.723246 6:-103166763 None:intergenic
TCTATGAATGTAGCAATGAG+TGG 0.727295 6:+103168615 MsaT033948.1:CDS
ATGATGATGATGATCACCAG+GGG 0.774959 6:-103164594 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 103164566 103168941 103164566 ID=MsaG033948
Chr6 mRNA 103164566 103168941 103164566 ID=MsaT033948.1;Parent=MsaG033948
Chr6 exon 103164566 103164705 103164566 ID=MsaT033948.1.exon1;Parent=MsaT033948.1
Chr6 CDS 103164566 103164705 103164566 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103164851 103164968 103164851 ID=MsaT033948.1.exon2;Parent=MsaT033948.1
Chr6 CDS 103164851 103164968 103164851 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103165390 103165578 103165390 ID=MsaT033948.1.exon3;Parent=MsaT033948.1
Chr6 CDS 103165390 103165578 103165390 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103166121 103166591 103166121 ID=MsaT033948.1.exon4;Parent=MsaT033948.1
Chr6 CDS 103166121 103166591 103166121 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103166682 103166783 103166682 ID=MsaT033948.1.exon5;Parent=MsaT033948.1
Chr6 CDS 103166682 103166783 103166682 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103166929 103167138 103166929 ID=MsaT033948.1.exon6;Parent=MsaT033948.1
Chr6 CDS 103166929 103167138 103166929 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103167222 103167396 103167222 ID=MsaT033948.1.exon7;Parent=MsaT033948.1
Chr6 CDS 103167222 103167396 103167222 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103167692 103167957 103167692 ID=MsaT033948.1.exon8;Parent=MsaT033948.1
Chr6 CDS 103167692 103167957 103167692 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103168059 103168148 103168059 ID=MsaT033948.1.exon9;Parent=MsaT033948.1
Chr6 CDS 103168059 103168148 103168059 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Chr6 exon 103168519 103168941 103168519 ID=MsaT033948.1.exon10;Parent=MsaT033948.1
Chr6 CDS 103168519 103168941 103168519 ID=cds.MsaT033948.1;Parent=MsaT033948.1
Gene Sequence

>MsaT033948.1

ATGGAGTATCATAGTGGAGCAAGTGGGTCCCCTGGTGATCATCATCATCATCATCATCACCATGAAGGTTCTTCTGATTCTCAGAGAAGAAAGAAACGTTACCACCGTCACACTGCTAATCAGATTCAAAGGCTTGAAGCTATGTTCAAGGAGTGTCCTCATCCTGATGAGAAACAAAGGTTGCAGTTAAGCAGAGAATTAGCTTTGGCTCCTAGACAGATCAAATTTTGGTTTCAGAACAGAAGGACTCAGATGAAGGCACAGCATGAAAGAGCTGATAACTGTCAACTAAGGCAAGAGAATGATAAAATAAGATGTGAGAATATAGCTATAAGGGAAGCCCTTAAAAATGTGATCTGCCCCTCTTGTGGTGGTCCTCCAATCAATGATGATTGTTTCTTTGATGAGCAAAAGCTGAGAATTGAAAATGTGCAACTCAAAGAAGAGCTTGATAGAGTATCAAGCATTGCTGCCAAATACATTGGAAGACCAATCACACAGCTTCCACCAGTTGCACCAATTCACATTTCATCATTGGACTTATCAATGGGAAATTTTGGTGGGCATGGAATGGTTGGAGGACCTTCTCTTGATCTTGATCTTCTACCTGGAAGTTCATCAACTATGCAGCATATTCCTTACCAACCAGCTTGTCTTTCTGACATGGAAAAATCTCTCATGTCTGACATTGCATCAAATGCAATGGAAGAGATGATTAGGCTTTTGCAGACTAATGAGCCTCTGTGGATGAAATCAAACTCTGATGGTAAAGATGTTCTTAATCTTGAAAACTATGAGAGGATGTTCCCTAAGACTAATAATCGGTTGAAGAATCCGAATATTCGCATTGAAGCTTCGAGGGATTCTCGTGTTGTTATCATGAATGGCTTAGCTTTGGTTGAAATGTTTATGGATCCAAACCAGTGGATGGCTCTCTTTCCTACCATTGTGACAATGGCAAGGACTATTGAAGTTATCTCTTCTGGAATGATGGGTGGTCCTAGTGGTTCTATGCAACTGATGTATGAGGAGTTGCAAATGCTGTCACCACTTGTTCCTATCAGAGAATTCTACTTCTTGCGTTACTGTACGCAAATTGAGCAAGGTTTATGGGCAATTGTTGATGTTTCCTATGATTTTCCTCATGACAACCAATTTGCTCCTCAATTTCGATGCCATCGTCTTCCATCTGGCTGCTTCATTCAAGACATGCCTAATGGCTACTCCAAGGTCACTTGGATTGAACATTTGGAGATTGAAGACAAAAATCCAGTTCATAGGCTTTATAGAAATGCTATCTATAGTGGAGCTGCATTTGGAGCTGAAAGATGGCTCACTACTCTTCAGAGAATGTGTGAAAGGCTTGCTTGTTCTATGGTTTCAGCCACCCGTGATCTCGGTGGAGCGATTCCATCACCTGAGGGAAAGAGCAGCATGATGAAGCTAGCACAAAGGATGGTAACTAATTTCTGTGCAAGCATAAGTGCATCAGCTAGTCATAGATGGACCACACTTTCAGCTTTGAATGAGGGAGTCAGAGTTACTGTTAGGAAGAGTACTGAACTTGGACAGCCTAGTGGTGTTGTGCTATGTGCAGCTACTACCATTTGGTTACCTCTCCCTCCACAAACTGTTTTCAATTTCTTCAAAGATGAAAGGAAAAGATCACAGTGGGATGTTCTTTCTAATGGGAATGCTGTCCAAGAAGTTGCTCATATTGCAAATGGTTCTCACCCTGGCAACTCCATCTCTGTGCTTAGGGCTTTCAACACAAGTCAAAACAACATGTTGATACTTCAAGAAAGCTGTGTTGATTCATCAGGTTCAATTGTAGTCTATTGTCCAATTGAATTAACATCTATGAATGTAGCAATGAGTGGTGAAGACACTTCCCACATTCCAGTAACACCTTCAGGTTTCACAATCACTTCTGATGGAAACACACTTGATAACAATAATAATCTTCAAGGAAGTGGTAATGTTAATGCTGATGGAGCATCAACAAGCTCAAACCTAGGGAATAATGGTGGTAGTGGAGGATCGTTGATAACAGTTGCATTCCAAATTATGGTTAGTAGTTTACCATCTGCTAAACTTAACATGGAGTCTGTTGCAACTGTTAATGGACTTATTGGTGAGACTGTTCAGCACATAAAAGCTGCTTTGAATTGCCCTAGTTCTTGA
Protein sequence

>MsaT033948.1

MEYHSGASGSPGDHHHHHHHHEGSSDSQRRKKRYHRHTANQIQRLEAMFKECPHPDEKQRLQLSRELALAPRQIKFWFQNRRTQMKAQHERADNCQLRQENDKIRCENIAIREALKNVICPSCGGPPINDDCFFDEQKLRIENVQLKEELDRVSSIAAKYIGRPITQLPPVAPIHISSLDLSMGNFGGHGMVGGPSLDLDLLPGSSSTMQHIPYQPACLSDMEKSLMSDIASNAMEEMIRLLQTNEPLWMKSNSDGKDVLNLENYERMFPKTNNRLKNPNIRIEASRDSRVVIMNGLALVEMFMDPNQWMALFPTIVTMARTIEVISSGMMGGPSGSMQLMYEELQMLSPLVPIREFYFLRYCTQIEQGLWAIVDVSYDFPHDNQFAPQFRCHRLPSGCFIQDMPNGYSKVTWIEHLEIEDKNPVHRLYRNAIYSGAAFGAERWLTTLQRMCERLACSMVSATRDLGGAIPSPEGKSSMMKLAQRMVTNFCASISASASHRWTTLSALNEGVRVTVRKSTELGQPSGVVLCAATTIWLPLPPQTVFNFFKDERKRSQWDVLSNGNAVQEVAHIANGSHPGNSISVLRAFNTSQNNMLILQESCVDSSGSIVVYCPIELTSMNVAMSGEDTSHIPVTPSGFTITSDGNTLDNNNNLQGSGNVNADGASTSSNLGNNGGSGGSLITVAFQIMVSSLPSAKLNMESVATVNGLIGETVQHIKAALNCPSS*