AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034136 MtrunA17_Chr6g0483051 82.567 631 61 3 385 967 6 635 0.0 1022
MsaG034136 MtrunA17_Chr6g0483071 83.333 240 28 5 1 234 1 234 2.93e-109 337
MsaG034136 MtrunA17_Chr6g0483061 69.565 115 4 1 301 384 1 115 1.00e-44 158
MsaG034136 MtrunA17_Chr3g0139671 36.111 144 78 3 450 579 35 178 2.51e-20 97.1
MsaG034136 MtrunA17_Chr3g0139671 32.667 150 87 3 396 532 34 182 7.32e-18 89.4
MsaG034136 MtrunA17_Chr3g0139671 31.538 130 76 3 364 482 55 182 5.15e-13 73.6
MsaG034136 MtrunA17_Chr3g0139671 42.105 95 53 2 487 579 33 127 1.22e-12 72.4
MsaG034136 MtrunA17_Chr7g0238631 36.364 143 77 3 451 579 64 206 8.25e-20 94.7
MsaG034136 MtrunA17_Chr7g0238631 35.065 154 86 3 396 536 62 214 1.42e-18 90.5
MsaG034136 MtrunA17_Chr7g0238631 39.474 114 65 3 470 579 42 155 1.26e-15 81.3
MsaG034136 MtrunA17_Chr7g0238631 32.308 130 75 3 364 482 83 210 3.47e-12 70.5
MsaG034136 MtrunA17_Chr5g0397841 38.255 149 75 4 448 579 55 203 1.19e-19 93.6
MsaG034136 MtrunA17_Chr5g0397841 30.872 149 88 3 395 528 55 203 1.14e-14 77.8
MsaG034136 MtrunA17_Chr1g0157731 34.722 144 80 3 450 579 41 184 3.30e-19 93.6
MsaG034136 MtrunA17_Chr1g0157731 33.333 150 86 3 396 532 40 188 7.68e-19 92.4
MsaG034136 MtrunA17_Chr1g0157731 43.158 95 52 2 487 579 39 133 1.27e-13 75.5
MsaG034136 MtrunA17_Chr1g0157731 30.769 130 77 3 364 482 61 188 2.44e-12 71.2
MsaG034136 MtrunA17_Chr4g0058991 42.157 102 56 2 479 579 203 302 1.03e-14 77.8
MsaG034136 MtrunA17_Chr5g0429661 39.362 94 56 1 487 579 122 215 2.22e-14 75.9
MsaG034136 MtrunA17_Chr7g0228531 40.000 120 65 3 485 603 218 331 2.76e-13 73.2
MsaG034136 MtrunA17_Chr7g0228531 33.333 96 52 1 445 528 217 312 2.33e-11 67.0
MsaG034136 MtrunA17_Chr3g0117511 40.426 94 55 1 487 579 183 276 3.95e-13 72.8
MsaG034136 MtrunA17_Chr5g0417621 31.538 130 86 2 487 615 134 261 6.65e-13 72.0
MsaG034136 MtrunA17_Chr4g0008551 40.426 94 55 1 487 579 38 131 1.17e-12 70.5
MsaG034136 MtrunA17_Chr4g0053431 36.170 94 59 1 487 579 5 98 2.99e-12 68.2
MsaG034136 MtrunA17_Chr5g0401731 37.234 94 58 1 487 579 15 108 9.31e-12 67.4
MsaG034136 MtrunA17_Chr4g0042501 28.767 146 99 3 487 629 105 248 4.67e-11 66.2
MsaG034136 MtrunA17_Chr6g0480581 34.021 97 63 1 487 582 4 100 6.17e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034136 AT3G18100.1 43.535 611 252 9 75 621 100 681 9.81e-157 483
MsaG034136 AT3G18100.2 49.682 471 183 6 202 621 1 468 4.32e-149 456
MsaG034136 AT3G18100.4 49.576 472 184 6 201 621 52 520 4.82e-149 458
MsaG034136 AT3G18100.3 38.512 605 281 13 75 621 62 633 1.20e-119 384
MsaG034136 AT5G11510.3 32.418 182 99 4 450 617 31 202 5.82e-23 106
MsaG034136 AT5G11510.3 32.667 150 87 3 396 532 30 178 3.65e-18 90.5
MsaG034136 AT5G11510.3 30.556 144 86 4 350 482 38 178 1.16e-12 72.8
MsaG034136 AT5G11510.1 32.418 182 99 4 450 617 31 202 5.82e-23 106
MsaG034136 AT5G11510.1 32.667 150 87 3 396 532 30 178 3.65e-18 90.5
MsaG034136 AT5G11510.1 30.556 144 86 4 350 482 38 178 1.16e-12 72.8
MsaG034136 AT5G11510.4 32.418 182 99 4 450 617 31 202 5.82e-23 106
MsaG034136 AT5G11510.4 32.667 150 87 3 396 532 30 178 3.65e-18 90.5
MsaG034136 AT5G11510.4 30.556 144 86 4 350 482 38 178 1.16e-12 72.8
MsaG034136 AT5G11510.5 32.418 182 99 4 450 617 31 202 7.84e-23 105
MsaG034136 AT5G11510.5 32.468 154 90 3 396 536 30 182 4.99e-18 90.1
MsaG034136 AT5G11510.5 30.556 144 86 4 350 482 38 178 1.74e-12 72.0
MsaG034136 AT5G11510.2 32.418 182 99 4 450 617 31 202 8.27e-23 105
MsaG034136 AT5G11510.2 32.667 150 87 3 396 532 30 178 5.15e-18 90.1
MsaG034136 AT5G11510.2 30.556 144 86 4 350 482 38 178 1.65e-12 72.0
MsaG034136 AT5G02320.3 37.063 143 76 3 451 579 18 160 8.88e-21 97.4
MsaG034136 AT5G02320.3 33.974 156 89 3 396 538 16 170 1.53e-19 93.6
MsaG034136 AT5G02320.3 40.952 105 58 3 479 579 5 109 5.93e-16 82.4
MsaG034136 AT5G02320.3 32.558 129 74 3 365 482 38 164 1.70e-12 71.6
MsaG034136 AT5G02320.1 37.063 143 76 3 451 579 78 220 1.44e-20 97.4
MsaG034136 AT5G02320.1 33.974 156 89 3 396 538 76 230 2.23e-19 93.6
MsaG034136 AT5G02320.1 40.952 105 58 3 479 579 65 169 8.36e-16 82.4
MsaG034136 AT5G02320.1 32.558 129 74 3 365 482 98 224 1.95e-12 71.6
MsaG034136 AT5G02320.2 37.063 143 76 3 451 579 78 220 1.44e-20 97.4
MsaG034136 AT5G02320.2 33.974 156 89 3 396 538 76 230 2.23e-19 93.6
MsaG034136 AT5G02320.2 40.952 105 58 3 479 579 65 169 8.36e-16 82.4
MsaG034136 AT5G02320.2 32.558 129 74 3 365 482 98 224 1.95e-12 71.6
MsaG034136 AT3G09370.2 36.364 143 77 3 451 579 86 228 3.69e-19 92.8
MsaG034136 AT3G09370.2 36.000 150 82 3 396 532 84 232 1.69e-18 90.5
MsaG034136 AT3G09370.2 45.000 100 51 3 484 579 78 177 3.19e-16 83.6
MsaG034136 AT3G09370.2 32.558 129 74 3 365 482 106 232 4.63e-12 70.1
MsaG034136 AT3G09370.1 36.364 143 77 3 451 579 81 223 4.06e-19 92.4
MsaG034136 AT3G09370.1 36.000 150 82 3 396 532 79 227 1.76e-18 90.5
MsaG034136 AT3G09370.1 45.000 100 51 3 484 579 73 172 3.33e-16 83.2
MsaG034136 AT3G09370.1 32.558 129 74 3 365 482 101 227 5.03e-12 70.1
MsaG034136 AT3G09370.3 36.364 143 77 3 451 579 94 236 4.71e-19 92.4
MsaG034136 AT3G09370.3 36.000 150 82 3 396 532 92 240 3.64e-18 89.7
MsaG034136 AT3G09370.3 45.000 100 51 3 484 579 86 185 3.83e-16 83.2
MsaG034136 AT3G09370.3 32.558 129 74 3 365 482 114 240 6.74e-12 69.7
MsaG034136 AT4G32730.5 29.891 184 105 5 450 619 37 210 3.02e-18 90.9
MsaG034136 AT4G32730.5 32.000 150 88 3 396 532 36 184 7.85e-18 89.7
MsaG034136 AT4G32730.5 42.105 95 53 2 487 579 35 129 1.03e-12 73.2
MsaG034136 AT4G32730.5 29.231 130 79 3 364 482 57 184 4.96e-12 70.9
MsaG034136 AT4G32730.4 29.891 184 105 5 450 619 37 210 3.02e-18 90.9
MsaG034136 AT4G32730.4 32.000 150 88 3 396 532 36 184 7.85e-18 89.7
MsaG034136 AT4G32730.4 42.105 95 53 2 487 579 35 129 1.03e-12 73.2
MsaG034136 AT4G32730.4 29.231 130 79 3 364 482 57 184 4.96e-12 70.9
MsaG034136 AT4G32730.3 29.891 184 105 5 450 619 37 210 3.02e-18 90.9
MsaG034136 AT4G32730.3 32.000 150 88 3 396 532 36 184 7.85e-18 89.7
MsaG034136 AT4G32730.3 42.105 95 53 2 487 579 35 129 1.03e-12 73.2
MsaG034136 AT4G32730.3 29.231 130 79 3 364 482 57 184 4.96e-12 70.9
MsaG034136 AT4G32730.2 29.891 184 105 5 450 619 37 210 3.02e-18 90.9
MsaG034136 AT4G32730.2 32.000 150 88 3 396 532 36 184 7.85e-18 89.7
MsaG034136 AT4G32730.2 42.105 95 53 2 487 579 35 129 1.03e-12 73.2
MsaG034136 AT4G32730.2 29.231 130 79 3 364 482 57 184 4.96e-12 70.9
MsaG034136 AT4G32730.1 29.891 184 105 5 450 619 37 210 3.24e-18 90.5
MsaG034136 AT4G32730.1 32.000 150 88 3 396 532 36 184 6.22e-18 89.7
MsaG034136 AT4G32730.1 42.105 95 53 2 487 579 35 129 1.08e-12 72.8
MsaG034136 AT4G32730.1 29.231 130 79 3 364 482 57 184 5.72e-12 70.5
MsaG034136 AT4G00540.2 31.746 189 88 5 396 579 47 199 1.78e-16 83.2
MsaG034136 AT4G00540.1 31.746 189 88 5 396 579 45 197 3.02e-16 82.8
MsaG034136 AT3G27785.1 41.667 96 55 1 485 579 187 282 1.88e-14 77.4
MsaG034136 AT5G11050.1 36.458 96 60 1 485 579 103 198 9.72e-13 72.0
MsaG034136 AT4G18770.1 39.362 94 56 1 487 579 217 310 1.25e-12 71.6
MsaG034136 AT5G67300.1 36.170 94 59 1 487 579 6 99 1.45e-12 70.1
MsaG034136 AT1G17950.1 37.113 97 60 1 487 582 5 101 6.21e-12 67.4
MsaG034136 AT3G55730.1 39.362 94 56 1 487 579 56 149 3.30e-11 67.0
MsaG034136 AT1G69560.1 35.354 99 63 1 485 582 105 203 3.41e-11 66.2
MsaG034136 AT2G39880.1 38.298 94 57 1 487 579 50 143 3.74e-11 66.6
MsaG034136 AT5G17800.1 35.052 97 62 1 487 582 93 189 3.75e-11 66.2
MsaG034136 AT1G79180.2 26.946 167 98 3 388 545 9 160 4.36e-11 65.9
MsaG034136 AT1G69560.2 35.354 99 63 1 485 582 121 219 4.69e-11 65.9
MsaG034136 AT5G39700.1 36.735 98 61 1 486 582 55 152 5.56e-11 63.2
MsaG034136 AT5G58850.1 34.314 102 66 1 479 579 97 198 6.33e-11 66.2
MsaG034136 AT5G40360.1 36.458 96 60 1 485 579 156 251 6.58e-11 65.9

Find 227 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCAACCTTTGATTTATT+TGG 0.096240 6:+106623140 None:intergenic
CTCTCCTTCCAAATTGAAAA+AGG 0.121481 6:-106618349 MsaT034136.1:CDS
TTGCAGATGCTTTCTTATTA+TGG 0.166772 6:+106622569 None:intergenic
ACTTACTTCTAAGAAATTTC+TGG 0.179080 6:+106623324 None:intergenic
ATCTAAAAGACGTGCTAAAA+AGG 0.216264 6:-106618829 MsaT034136.1:CDS
AACCTTATTGAATCAGTTAA+AGG 0.235058 6:-106622230 MsaT034136.1:CDS
GATACGGCCAGGAACATATT+TGG 0.246910 6:+106619880 None:intergenic
AGACCAGCAACATCCATATT+TGG 0.276579 6:+106619674 None:intergenic
GACCTTTAACTGATTCAATA+AGG 0.285862 6:+106622228 None:intergenic
AATCGATCCCAATTAACTTT+AGG 0.296877 6:+106622170 None:intergenic
GAAGCTGCAATCACCAAATA+TGG 0.298716 6:-106619687 MsaT034136.1:CDS
TTCTCGATCGACAAAGTTCC+TGG 0.302389 6:+106619428 None:intergenic
TGGCAGATACCTTAATAGTT+TGG 0.316146 6:-106619760 MsaT034136.1:intron
TTGTCTCTTACATGTGTTAC+TGG 0.325805 6:-106618567 MsaT034136.1:CDS
TCAACGACATTGATGTTTGA+TGG 0.327079 6:+106618210 None:intergenic
AAGAAGGGTCCAAACTATTA+AGG 0.329261 6:+106619751 None:intergenic
AATCCGTCTTGCTCATTAGA+TGG 0.331360 6:+106618588 None:intergenic
TTCAACTTGCTGTCATTCTC+AGG 0.333946 6:+106623482 None:intergenic
CATGCTTTGGTCAGTGTGAT+TGG 0.337147 6:-106620977 MsaT034136.1:intron
GAACTCTCTTTAGACATTCT+AGG 0.346245 6:+106625496 None:intergenic
GGATCAGGCCTCGAGTTTAA+TGG 0.348097 6:-106623579 MsaT034136.1:CDS
GTTTGGACCCTTCTTTGAAA+TGG 0.349718 6:-106619743 MsaT034136.1:CDS
GGACAATGTTAAACTCCTTA+AGG 0.355715 6:-106623258 MsaT034136.1:CDS
AGCAAAGGTCTCAGCTTTCC+AGG 0.357183 6:-106619446 MsaT034136.1:CDS
TTCTCAGGGTCTTGAGAATC+AGG 0.359272 6:+106623497 None:intergenic
TAAACTCCTTAAGGATTTCC+AGG 0.364402 6:-106623249 MsaT034136.1:CDS
GCAACAACTTTATTTCCTAA+TGG 0.374157 6:+106618059 None:intergenic
TCCTTCGCCGGTGCCGATGA+TGG 0.374712 6:-106625442 MsaT034136.1:CDS
TGGAAAGGAAGCATGGGCAG+AGG 0.382172 6:-106623603 MsaT034136.1:CDS
TTGTCCAAGAGAAGGGTATC+AGG 0.390155 6:-106621140 MsaT034136.1:CDS
ACTGTAGCGGTCATGCTGTT+TGG 0.394003 6:-106619923 MsaT034136.1:CDS
CAGGCTTCCATTTCAATGCT+TGG 0.397187 6:-106621082 MsaT034136.1:CDS
ACTTTCTCTTCCGATTCCTT+CGG 0.403956 6:+106619322 None:intergenic
GTAAGGCGTTCGTCCTCCTC+AGG 0.404385 6:+106619944 None:intergenic
TGGCAAGGATAAGCCTCTTC+AGG 0.404536 6:-106618430 MsaT034136.1:CDS
CACAGTTCAGAGTGTTTACC+TGG 0.412332 6:-106622272 MsaT034136.1:intron
AGACATTCTAGGTCATCATC+AGG 0.413793 6:+106625507 None:intergenic
GCAACACGGGAATTCTCATC+TGG 0.414429 6:+106622535 None:intergenic
GTTTGACATTGCTGTATCAT+TGG 0.415041 6:-106621112 MsaT034136.1:CDS
TGAGAAGGAAAACAAAGAAA+TGG 0.416166 6:-106618401 MsaT034136.1:CDS
CCACCTTGCAAAACAAGATA+AGG 0.417091 6:-106618101 MsaT034136.1:CDS
CAAATAGCCAAATATGTTCC+TGG 0.418057 6:-106619887 MsaT034136.1:CDS
AAAGCATCTGCAATGTGTTA+TGG 0.421920 6:-106622559 MsaT034136.1:CDS
ATATGGATGTTGCTGGTCTA+AGG 0.424272 6:-106619670 MsaT034136.1:CDS
CTTCTTTGAAATGGAGTGAA+TGG 0.428476 6:-106619734 MsaT034136.1:CDS
ACCTGTCCCTACACTGAGAT+TGG 0.432516 6:+106619858 None:intergenic
CTGAAAATCTCCGAAGGAAT+CGG 0.437709 6:-106619332 MsaT034136.1:CDS
TTAAGCAGAAGAGCTTGTTC+AGG 0.440920 6:+106619483 None:intergenic
TCACCAAATATGGATGTTGC+TGG 0.441147 6:-106619677 MsaT034136.1:CDS
CTGTATCATTGGCCACAAAC+AGG 0.441542 6:-106621101 MsaT034136.1:CDS
ATCCTCATTGGCGGTGGCAG+TGG 0.445074 6:+106625536 None:intergenic
CACTAGAATCAACTTCTGTC+TGG 0.446682 6:+106618523 None:intergenic
TCATGCTGTTTGGACGGAAA+TGG 0.447710 6:-106619913 MsaT034136.1:CDS
ATGAAACCTGGAAATCCTTA+AGG 0.447834 6:+106623243 None:intergenic
TCCTAGAGAGAGACGAAGTC+TGG 0.449339 6:+106618646 None:intergenic
TCCATGTCATTTGCATCTCC+AGG 0.450089 6:+106622254 None:intergenic
CATTCACTCCATTTCAAAGA+AGG 0.450882 6:+106619735 None:intergenic
TAAAGTTAATTGGGATCGAT+TGG 0.454166 6:-106622168 MsaT034136.1:CDS
TTCAATTTCGTCTTCATCGG+CGG 0.455242 6:+106625611 None:intergenic
TTCTTCAATTTCGTCTTCAT+CGG 0.459661 6:+106625608 None:intergenic
TTCTTCCATGTTGATGTCTG+TGG 0.462817 6:+106618146 None:intergenic
AATCGTGCCAAGCATTGAAA+TGG 0.463782 6:+106621075 None:intergenic
ATCGGAGGAGGTGGCGGAGA+GGG 0.463824 6:+106625692 None:intergenic
CAATTTGTCCTGTGAGGAAA+GGG 0.467162 6:-106619976 MsaT034136.1:CDS
AGTTCCTGATACCCTTCTCT+TGG 0.467311 6:+106621136 None:intergenic
AGGAGGTGGCGGAGAGGGAA+TGG 0.468689 6:+106625697 None:intergenic
GACCGCATCAATAAAAGCTT+GGG 0.469114 6:+106623364 None:intergenic
TGACTTAATTCCTTCACAAA+TGG 0.469153 6:-106619379 MsaT034136.1:CDS
CTTCTCAATTACCGAAGAAA+AGG 0.474981 6:-106623406 MsaT034136.1:CDS
GAAGGAATTAAGTCATTCAC+TGG 0.476334 6:+106619387 None:intergenic
TGACCGCATCAATAAAAGCT+TGG 0.479293 6:+106623363 None:intergenic
TGCTAACTATAAGATGGTAT+TGG 0.482158 6:-106622515 MsaT034136.1:CDS
CTCGAGTTTAATGGATGAGC+AGG 0.483115 6:-106623570 MsaT034136.1:CDS
GGGGAATTCTGTTCCTTCGC+CGG 0.486482 6:-106625454 MsaT034136.1:CDS
TCTTCCATGTTGATGTCTGT+GGG 0.486592 6:+106618147 None:intergenic
AAGAGAAGGGTATCAGGAAC+TGG 0.487054 6:-106621134 MsaT034136.1:CDS
GGAAAGGAAGCATGGGCAGA+GGG 0.488737 6:-106623602 MsaT034136.1:CDS
ATGGGCCATGGTTGATCAAA+GGG 0.489303 6:+106621196 None:intergenic
ACTTTCCGAACTCTTGCTAC+AGG 0.490694 6:+106618749 None:intergenic
TTCGTCCTCCTCAGGGGTAA+AGG 0.491814 6:+106619952 None:intergenic
ATGTATGCCACTGGCCGTAC+TGG 0.492414 6:-106622140 MsaT034136.1:CDS
GAGACCATCGTCGTCGTCAT+CGG 0.492841 6:+106625674 None:intergenic
GTCGTCATCGGAGGAGGTGG+CGG 0.493389 6:+106625686 None:intergenic
CGGGAACCCAGTGCTCGAAT+AGG 0.493876 6:-106620923 MsaT034136.1:intron
CTTCGATTATAAATAGTGAA+TGG 0.496259 6:-106621035 MsaT034136.1:CDS
CGGTGGCAGTGGAGGTGAAA+GGG 0.498619 6:+106625547 None:intergenic
CTTCAGAAGTCCATGGGCCA+TGG 0.498659 6:+106621184 None:intergenic
AAGGGATCTATGTTACTCAA+TGG 0.498782 6:-106618330 MsaT034136.1:CDS
ATCCGTCTTGCTCATTAGAT+GGG 0.504207 6:+106618589 None:intergenic
TTTGATTATTGTCCAAGAGA+AGG 0.507766 6:-106621148 MsaT034136.1:CDS
TTATAAATAGTGAATGGACT+GGG 0.510551 6:-106621029 MsaT034136.1:CDS
CTTCTCACATAGACCTGAAG+AGG 0.511308 6:+106618417 None:intergenic
TCAATAAAAGCTTGGGCCGA+TGG 0.518217 6:+106623371 None:intergenic
AAGTCATTCACTGGAAGGAC+GGG 0.518743 6:+106619396 None:intergenic
CCTTCGCCGGTGCCGATGAT+GGG 0.519488 6:-106625441 MsaT034136.1:CDS
GAACCAACCTATTCGAGCAC+TGG 0.520039 6:+106620916 None:intergenic
CAAGACCTGTAGCAAGAGTT+CGG 0.520718 6:-106618754 MsaT034136.1:CDS
AAGGTCAAGCTTTCCTCCAT+CGG 0.523147 6:-106623387 MsaT034136.1:CDS
CTCATTGGCGGTGGCAGTGG+AGG 0.523736 6:+106625539 None:intergenic
CAGTCTCTCTACAAACCTAC+GGG 0.524073 6:-106625474 MsaT034136.1:CDS
TTGATTATTGTCCAAGAGAA+GGG 0.525222 6:-106621147 MsaT034136.1:CDS
TCTGCATCGTGTACTTCCTC+AGG 0.527014 6:+106619072 None:intergenic
TCAATTTGTCCTGTGAGGAA+AGG 0.527537 6:-106619977 MsaT034136.1:CDS
CCCATCATCGGCACCGGCGA+AGG 0.527852 6:+106625441 None:intergenic
ACCTCTGACAACTCAGATGC+CGG 0.528355 6:-106623530 MsaT034136.1:CDS
GTCGTCGTCATCGGAGGAGG+TGG 0.528578 6:+106625683 None:intergenic
GTAAAGGTCCCTTTCCTCAC+AGG 0.529986 6:+106619968 None:intergenic
GAGGATGCACATAATATGAA+TGG 0.530539 6:-106618456 MsaT034136.1:CDS
ATCGTCGTCGTCATCGGAGG+AGG 0.530850 6:+106625680 None:intergenic
CCGGCATTGACAATCAGTGC+GGG 0.533494 6:+106619629 None:intergenic
TCAACTTGCTGTCATTCTCA+GGG 0.533513 6:+106623483 None:intergenic
ATTATAAATAGTGAATGGAC+TGG 0.534017 6:-106621030 MsaT034136.1:CDS
AATGCTTGGCACGATTTCAA+AGG 0.535163 6:-106621068 MsaT034136.1:CDS
ACATCGCTTTGCTCAGACAA+TGG 0.537325 6:+106618867 None:intergenic
CACTGAGATTGGATACGGCC+AGG 0.538017 6:+106619869 None:intergenic
CATGGGCCATGGTTGATCAA+AGG 0.541123 6:+106621195 None:intergenic
GCCAGACTTCGTCTCTCTCT+AGG 0.542228 6:-106618647 MsaT034136.1:CDS
CATTCACTATTTATAATCGA+AGG 0.542434 6:+106621036 None:intergenic
AAAATTGTATCTAAGCAGGT+TGG 0.543436 6:-106618621 MsaT034136.1:CDS
AAACATCAATGTCGTTGAAG+AGG 0.543823 6:-106618205 MsaT034136.1:CDS
CCCTACACTGAGATTGGATA+CGG 0.544793 6:+106619864 None:intergenic
GAAAGGGACAATAGCGTCAC+CGG 0.546779 6:+106625563 None:intergenic
CCCGCACTGATTGTCAATGC+CGG 0.546948 6:-106619629 MsaT034136.1:CDS
CCGTGTTGCTAACTATAAGA+TGG 0.547911 6:-106622521 MsaT034136.1:CDS
TCCAATCTCAGTGTAGGGAC+AGG 0.550474 6:-106619859 MsaT034136.1:intron
TGTTCGTATTCGTCGGTGCG+TGG 0.551737 6:+106625732 None:intergenic
ATCAGGGTCAGAATCCTCAT+TGG 0.551780 6:+106625524 None:intergenic
ACAGCATGACCGCTACAGTA+AGG 0.553023 6:+106619927 None:intergenic
TAAGTCATTCACTGGAAGGA+CGG 0.556782 6:+106619395 None:intergenic
TTATCCTCTTCAGAAGTCCA+TGG 0.556985 6:+106621177 None:intergenic
CTTTGATCAACCATGGCCCA+TGG 0.557051 6:-106621194 MsaT034136.1:CDS
ACGACATCACACTTGCATGC+TGG 0.557543 6:-106618716 MsaT034136.1:CDS
GCCGTATCCAATCTCAGTGT+AGG 0.557965 6:-106619865 MsaT034136.1:CDS
GCGGTGGCAGTGGAGGTGAA+AGG 0.559657 6:+106625546 None:intergenic
TAAGGCGTTCGTCCTCCTCA+GGG 0.560504 6:+106619945 None:intergenic
TCAGTCTCTCTACAAACCTA+CGG 0.561114 6:-106625475 MsaT034136.1:CDS
TTCACTGCCGTCAGAAATGA+TGG 0.561447 6:+106618296 None:intergenic
TTGAAATGGAAGCCTGTTTG+TGG 0.562015 6:+106621089 None:intergenic
CCGTATCCAATCTCAGTGTA+GGG 0.562137 6:-106619864 MsaT034136.1:CDS
TTGAAGGTGGAAAGGAAGCA+TGG 0.562281 6:-106623610 MsaT034136.1:intron
AAAGGGTCGAATTCATCTCA+AGG 0.563869 6:-106618811 MsaT034136.1:CDS
ATTCACTCCATTTCAAAGAA+GGG 0.563989 6:+106619736 None:intergenic
GAAAGGAAGCATGGGCAGAG+GGG 0.564022 6:-106623601 MsaT034136.1:CDS
AAAGGAAGCATGGGCAGAGG+GGG 0.565557 6:-106623600 MsaT034136.1:CDS
TTGGCTTCTATGTATGCCAC+TGG 0.566806 6:-106622149 MsaT034136.1:CDS
CTCTTCAGGTCTATGTGAGA+AGG 0.567546 6:-106618416 MsaT034136.1:CDS
GTCAGAATCCTCATTGGCGG+TGG 0.567681 6:+106625530 None:intergenic
GGTGGCGGAGAGGGAATGGT+CGG 0.568069 6:+106625701 None:intergenic
ATTTGGAAGGAGAGCAAGCA+CGG 0.571481 6:+106618358 None:intergenic
CTGCCGTCAGAAATGATGGT+TGG 0.573978 6:+106618300 None:intergenic
TAATATGAATGGTGATGGCA+AGG 0.579147 6:-106618445 MsaT034136.1:CDS
CTTGCAGTCACCAGAAAGAG+AGG 0.580959 6:-106619127 MsaT034136.1:intron
ACGTATTTAGAACGGCGGAC+GGG 0.581277 6:-106620942 MsaT034136.1:CDS
TTCTGCACCAAATAAATCAA+AGG 0.581623 6:-106623147 MsaT034136.1:intron
AACCAACCTATTCGAGCACT+GGG 0.582462 6:+106620917 None:intergenic
CGATGGTCTCAAAGATGACA+TGG 0.584029 6:-106625661 MsaT034136.1:CDS
GGCATCTGAGTTGTCAGAGG+TGG 0.585358 6:+106623532 None:intergenic
TATCCTCTTCAGAAGTCCAT+GGG 0.585905 6:+106621178 None:intergenic
AGGGTCAGAATCCTCATTGG+CGG 0.586291 6:+106625527 None:intergenic
TTTGCAAGGTGGAAAGAGGA+AGG 0.587368 6:+106618112 None:intergenic
CATGTCAAAAGTTGTTGTAG+AGG 0.587522 6:-106618268 MsaT034136.1:CDS
TACGTATTTAGAACGGCGGA+CGG 0.587695 6:-106620943 MsaT034136.1:CDS
TCATCATCATCACCCATCAT+CGG 0.587696 6:+106625429 None:intergenic
TGGTTCCCTTGAAGGTGGAA+AGG 0.589368 6:-106623618 MsaT034136.1:intron
ATTCACATTCAGCACCAGTA+CGG 0.592033 6:+106622126 None:intergenic
TCATCACCCATCATCGGCAC+CGG 0.592774 6:+106625435 None:intergenic
TTCATTGGTCAGGAGATGGA+TGG 0.593162 6:-106619514 MsaT034136.1:intron
TGAAGGTGGAAAGGAAGCAT+GGG 0.594158 6:-106623609 MsaT034136.1:intron
CTCCACTGCCACCGCCAATG+AGG 0.595663 6:-106625538 MsaT034136.1:CDS
GAGTGAATGGACTAAAGAAG+AGG 0.598139 6:-106619721 MsaT034136.1:CDS
GCACATAATATGAATGGTGA+TGG 0.601762 6:-106618450 MsaT034136.1:CDS
AAGAGTTCGGAAAGTCAAGA+TGG 0.602465 6:-106618741 MsaT034136.1:CDS
GACATTCTAGGTCATCATCA+GGG 0.605057 6:+106625508 None:intergenic
CATCGGAGGAGGTGGCGGAG+AGG 0.609122 6:+106625691 None:intergenic
AGAATCAGGACACAATTCAC+CGG 0.611779 6:+106623511 None:intergenic
TAGCGGTCATGCTGTTTGGA+CGG 0.612282 6:-106619919 MsaT034136.1:CDS
TCTCTTACATGTGTTACTGG+TGG 0.612638 6:-106618564 MsaT034136.1:CDS
TGGCCCATGGACTTCTGAAG+AGG 0.614566 6:-106621181 MsaT034136.1:CDS
ATAAAAGCTTGGGCCGATGG+AGG 0.615824 6:+106623374 None:intergenic
CAAGTCTCATCAGCACCACA+TGG 0.617617 6:+106623449 None:intergenic
ACCATCGTCGTCGTCATCGG+AGG 0.622403 6:+106625677 None:intergenic
GGTGGAACTACCATTTGTGA+AGG 0.623695 6:+106619369 None:intergenic
CATCTTATAGTTAGCAACAC+GGG 0.625194 6:+106622522 None:intergenic
CTGCTCATCCATTAAACTCG+AGG 0.629224 6:+106623571 None:intergenic
CTGGTGCTGAATGTGAATCA+AGG 0.629877 6:-106622121 MsaT034136.1:intron
GAATTAAGTCATTCACTGGA+AGG 0.629989 6:+106619391 None:intergenic
GAATATAGCATTTAGTGACA+AGG 0.631425 6:-106618778 MsaT034136.1:CDS
CAACACGGGAATTCTCATCT+GGG 0.633868 6:+106622536 None:intergenic
ACCTGGAGATGCAAATGACA+TGG 0.634244 6:-106622255 MsaT034136.1:CDS
AAATGCAGCTAGAAAATCAG+AGG 0.638930 6:-106618475 MsaT034136.1:CDS
ATCCCATCTAATGAGCAAGA+CGG 0.639074 6:-106618591 MsaT034136.1:CDS
AAGCCAACCATCATTTCTGA+CGG 0.643084 6:-106618303 MsaT034136.1:CDS
ATGCAGATCCCAAGAAAGAG+AGG 0.643195 6:-106619056 MsaT034136.1:CDS
CCATCTTATAGTTAGCAACA+CGG 0.644909 6:+106622521 None:intergenic
GAAGACCCTTTGATCAACCA+TGG 0.645219 6:-106621201 MsaT034136.1:CDS
AAATGGAGTGAAGATACTGA+AGG 0.645956 6:-106618384 MsaT034136.1:CDS
ACCGGCATCTGAGTTGTCAG+AGG 0.654962 6:+106623529 None:intergenic
TCAGCACCAACATCAGATGG+TGG 0.655822 6:+106619351 None:intergenic
GATGATATTAACTGCACACG+CGG 0.657130 6:+106623179 None:intergenic
TTCAGCACCAGTACGGCCAG+TGG 0.657884 6:+106622133 None:intergenic
GTAGTTCCACCATCTGATGT+TGG 0.658029 6:-106619357 MsaT034136.1:CDS
TTGGTGCTGAAAATCTCCGA+AGG 0.658248 6:-106619338 MsaT034136.1:CDS
GGACGAACGCCTTACTGTAG+CGG 0.658493 6:-106619936 MsaT034136.1:CDS
CTGTAGCTACGTATTTAGAA+CGG 0.659399 6:-106620950 MsaT034136.1:CDS
AGGGACCTTTACCCCTGAGG+AGG 0.664445 6:-106619957 MsaT034136.1:CDS
CGGCATTGACAATCAGTGCG+GGG 0.666902 6:+106619630 None:intergenic
AGGGACAATAGCGTCACCGG+TGG 0.670416 6:+106625566 None:intergenic
GAAGGAACAGAATTCCCCGT+AGG 0.671408 6:+106625459 None:intergenic
GCGTCACCGGTGGATAAAAG+CGG 0.676532 6:+106625576 None:intergenic
TCCGGCATTGACAATCAGTG+CGG 0.678171 6:+106619628 None:intergenic
TCCTCCGATGACGACGACGA+TGG 0.678470 6:-106625678 MsaT034136.1:CDS
AAGATGAAGACTTCTGATCG+AGG 0.680787 6:-106618894 MsaT034136.1:CDS
GATGATGGGTGATGATGATG+AGG 0.682359 6:-106625427 MsaT034136.1:CDS
TTGTCGATCGAGAATCTGAA+CGG 0.683321 6:-106619419 MsaT034136.1:CDS
TATAAATAGTGAATGGACTG+GGG 0.683823 6:-106621028 MsaT034136.1:CDS
GGAATCGGAAGAGAAAGTCA+AGG 0.696658 6:-106619317 MsaT034136.1:intron
AAGGCGTTCGTCCTCCTCAG+GGG 0.696660 6:+106619946 None:intergenic
AGAGACTGGACTTTGTCCTG+AGG 0.697235 6:-106619088 MsaT034136.1:CDS
ATGATATTAACTGCACACGC+GGG 0.699180 6:+106623180 None:intergenic
GAACAAGTGAGAAAACCAGA+AGG 0.701210 6:-106618501 MsaT034136.1:CDS
TTGAAATTGATTAAGCCATG+TGG 0.707688 6:-106623464 MsaT034136.1:CDS
TGAACCCACAGACATCAACA+TGG 0.723844 6:-106618151 MsaT034136.1:CDS
GAAGAGGAGACTGATCTGCA+TGG 0.727157 6:-106618189 MsaT034136.1:CDS
TAGCTACGTATTTAGAACGG+CGG 0.743423 6:-106620947 MsaT034136.1:CDS
GAAAGGGACCTTTACCCCTG+AGG 0.748237 6:-106619960 MsaT034136.1:CDS
GAGGAGACTGATCTGCATGG+TGG 0.779129 6:-106618186 MsaT034136.1:CDS
AGTCTCTCTACAAACCTACG+GGG 0.834290 6:-106625473 MsaT034136.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 106618068 106625760 106618068 ID=MsaG034136
Chr6 mRNA 106618068 106625760 106618068 ID=MsaT034136.1;Parent=MsaG034136
Chr6 exon 106618068 106618927 106618068 ID=MsaT034136.1.exon11;Parent=MsaT034136.1
Chr6 CDS 106618068 106618927 106618068 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106619033 106619142 106619033 ID=MsaT034136.1.exon10;Parent=MsaT034136.1
Chr6 CDS 106619033 106619142 106619033 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106619318 106619524 106619318 ID=MsaT034136.1.exon9;Parent=MsaT034136.1
Chr6 CDS 106619318 106619524 106619318 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106619627 106619776 106619627 ID=MsaT034136.1.exon8;Parent=MsaT034136.1
Chr6 CDS 106619627 106619776 106619627 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106619860 106620009 106619860 ID=MsaT034136.1.exon7;Parent=MsaT034136.1
Chr6 CDS 106619860 106620009 106619860 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106620924 106620985 106620924 ID=MsaT034136.1.exon6;Parent=MsaT034136.1
Chr6 CDS 106620924 106620985 106620924 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106621020 106621236 106621020 ID=MsaT034136.1.exon5;Parent=MsaT034136.1
Chr6 CDS 106621020 106621236 106621020 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106622122 106622284 106622122 ID=MsaT034136.1.exon4;Parent=MsaT034136.1
Chr6 CDS 106622122 106622284 106622122 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106622482 106622593 106622482 ID=MsaT034136.1.exon3;Parent=MsaT034136.1
Chr6 CDS 106622482 106622593 106622482 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106623148 106623626 106623148 ID=MsaT034136.1.exon2;Parent=MsaT034136.1
Chr6 CDS 106623148 106623626 106623148 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Chr6 exon 106625364 106625760 106625364 ID=MsaT034136.1.exon1;Parent=MsaT034136.1
Chr6 CDS 106625364 106625760 106625364 ID=cds.MsaT034136.1;Parent=MsaT034136.1
Gene Sequence

>MsaT034136.1

ATGTCACCACGCACCGACGAATACGAACAAGAAAACACCGACCATTCCCTCTCCGCCACCTCCTCCGATGACGACGACGATGGTCTCAAAGATGACATGGCAGCTCTCGCACGCGCTTGTAGATTCACCGCCGATGAAGACGAAATTGAAGAAGTAGAAGATCCGCTTTTATCCACCGGTGACGCTATTGTCCCTTTCACCTCCACTGCCACCGCCAATGAGGATTCTGACCCTGATGATGACCTAGAATGTCTAAAGAGAGTTCAGTCTCTCTACAAACCTACGGGGAATTCTGTTCCTTCGCCGGTGCCGATGATGGGTGATGATGATGAGGATGATGAAGATGATTTTGAAACTGTTCGCGCTATTTTCAAACGGTTTTCTGCTTACGATGGAGGTGGAAAGGAAGCATGGGCAGAGGGGGATCAGGCCTCGAGTTTAATGGATGAGCAGGATGTTGTCAAGAGCTCCACCTCTGACAACTCAGATGCCGGTGAATTGTGTCCTGATTCTCAAGACCCTGAGAATGACAGCAAGTTGAAATTGATTAAGCCATGTGGTGCTGATGAGACTTGTAAAAATGATGCGGGCTTGGCTTCTCAATTACCGAAGAAAAGGTCAAGCTTTCCTCCATCGGCCCAAGCTTTTATTGATGCGGTCAAGAAGAATAGAGCTCTCCAGAAATTTCTTAGAAGTAAGTTAATTGAAATTGAAGCAAAAATTGGGGAAAACAAAAAGATAAGGGACAATGTTAAACTCCTTAAGGATTTCCAGGTTTCATGCATTAGAAAAACGGGAAGTGCTTTTTCACTGAGAAAGGACCCGCGTGTGCAGTTAATATCATCCAAAAAGTCTTCTGCACCAAATAAATCAAAGAGCCATAATAAGAAAGCATCTGCAATGTGTTATGGCCCAGATGAGAATTCCCGTGTTGCTAACTATAAGATGGTATTGGAAAGATTTCCGCTATCATTGACTAAGAAAAATGAGTGTTTACCTGGAGATGCAAATGACATGGATAACCTTATTGAATCAGTTAAAGGTCTTGAAATCACACCTGCGATGATTAGAGAATTTTTACCTAAAGTTAATTGGGATCGATTGGCTTCTATGTATGCCACTGGCCGTACTGGTGCTGAATGTGAATCAAGGTGGTTGAATTGTGAAGACCCTTTGATCAACCATGGCCCATGGACTTCTGAAGAGGATAAATCTCTTTTGATTATTGTCCAAGAGAAGGGTATCAGGAACTGGTTTGACATTGCTGTATCATTGGCCACAAACAGGCTTCCATTTCAATGCTTGGCACGATTTCAAAGGAGCTTAAATCCTTCGATTATAAATAGTGAATGGACTGGGGAAGAAGATTGTGATTGGCAGTCTGTAGCTACGTATTTAGAACGGCGGACGGGAACCCAGTGCTCGAATAGATGGAAAAAATCAATTTGTCCTGTGAGGAAAGGGACCTTTACCCCTGAGGAGGACGAACGCCTTACTGTAGCGGTCATGCTGTTTGGACGGAAATGGAAGCAAATAGCCAAATATGTTCCTGGCCGTATCCAATCTCAGTGTAGGGACAGATACCTTAATAGTTTGGACCCTTCTTTGAAATGGAGTGAATGGACTAAAGAAGAGGATTCAAGATTAGAAGCTGCAATCACCAAATATGGATGTTGCTGGTCTAAGGTTGCTGAAGATGTGACTCCCCGCACTGATTGTCAATGCCGGAAGAGATGGATGGTGTTACATCCTGAACAAGCTCTTCTGCTTAAAGAAGCAAAAAAGAAGCAAAGGTCTCAGCTTTCCAGGAACTTTGTCGATCGAGAATCTGAACGGCCCGTCCTTCCAGTGAATGACTTAATTCCTTCACAAATGGTAGTTCCACCATCTGATGTTGGTGCTGAAAATCTCCGAAGGAATCGGAAGAGAAAGTCAAGTCACCAGAAAGAGAGGTCCAAAAAACATGCAAAGAGACTGGACTTTGTCCTGAGGAAGTACACGATGCAGATCCCAAGAAAGAGAGGCCTAAAAGACATGCAAAAAGAAGCATATAGTGATAAGATGAAGACTTCTGATCGAGGTGTTCCATTGTCTGAGCAAAGCGATGTTCTCAATAATATGAGATCTAAAAGACGTGCTAAAAAGGGTCGAATTCATCTCAAGGAAGTAGAGAAGAATATAGCATTTAGTGACAAGGTCAAGACCTGTAGCAAGAGTTCGGAAAGTCAAGATGGAGACGACATCACACTTGCATGCTGGCGCAACAAATTAAAGAAGAAACTATCTAACTGTACCAAAAATGCGAGCCAGACTTCGTCTCTCTCTAGGACAAAAATTGTATCTAAGCAGGTTGGAAATCAAATCCCATCTAATGAGCAAGACGGATTGTCTCTTACATGTGTTACTGGTGGAACTAAAGATTTGTTAATCCAGACAGAAGTTGATTCTAGTGAACAAGTGAGAAAACCAGAAGGTGAAAATGCAGCTAGAAAATCAGAGGATGCACATAATATGAATGGTGATGGCAAGGATAAGCCTCTTCAGGTCTATGTGAGAAGGAAAACAAAGAAATGGAGTGAAGATACTGAAGGATGCCGTGCTTGCTCTCCTTCCAAATTGAAAAAGGGATCTATGTTACTCAATGGAAACAAGCCAACCATCATTTCTGACGGCAGTGAACCATCCATGTCAAAAGTTGTTGTAGAGGAGACTGTTTTACATGGCGGTGTGGCTGAAGCTGAACCATCAAACATCAATGTCGTTGAAGAGGAGACTGATCTGCATGGTGGTGTTGCTCATGCTGAACCCACAGACATCAACATGGAAGAAAATGATGACGACCTTCCTCTTTCCACCTTGCAAAACAAGATAAGGAGACGAAAAAGACGGCACACCATTAGGAAATAA
Protein sequence

>MsaT034136.1

MSPRTDEYEQENTDHSLSATSSDDDDDGLKDDMAALARACRFTADEDEIEEVEDPLLSTGDAIVPFTSTATANEDSDPDDDLECLKRVQSLYKPTGNSVPSPVPMMGDDDEDDEDDFETVRAIFKRFSAYDGGGKEAWAEGDQASSLMDEQDVVKSSTSDNSDAGELCPDSQDPENDSKLKLIKPCGADETCKNDAGLASQLPKKRSSFPPSAQAFIDAVKKNRALQKFLRSKLIEIEAKIGENKKIRDNVKLLKDFQVSCIRKTGSAFSLRKDPRVQLISSKKSSAPNKSKSHNKKASAMCYGPDENSRVANYKMVLERFPLSLTKKNECLPGDANDMDNLIESVKGLEITPAMIREFLPKVNWDRLASMYATGRTGAECESRWLNCEDPLINHGPWTSEEDKSLLIIVQEKGIRNWFDIAVSLATNRLPFQCLARFQRSLNPSIINSEWTGEEDCDWQSVATYLERRTGTQCSNRWKKSICPVRKGTFTPEEDERLTVAVMLFGRKWKQIAKYVPGRIQSQCRDRYLNSLDPSLKWSEWTKEEDSRLEAAITKYGCCWSKVAEDVTPRTDCQCRKRWMVLHPEQALLLKEAKKKQRSQLSRNFVDRESERPVLPVNDLIPSQMVVPPSDVGAENLRRNRKRKSSHQKERSKKHAKRLDFVLRKYTMQIPRKRGLKDMQKEAYSDKMKTSDRGVPLSEQSDVLNNMRSKRRAKKGRIHLKEVEKNIAFSDKVKTCSKSSESQDGDDITLACWRNKLKKKLSNCTKNASQTSSLSRTKIVSKQVGNQIPSNEQDGLSLTCVTGGTKDLLIQTEVDSSEQVRKPEGENAARKSEDAHNMNGDGKDKPLQVYVRRKTKKWSEDTEGCRACSPSKLKKGSMLLNGNKPTIISDGSEPSMSKVVVEETVLHGGVAEAEPSNINVVEEETDLHGGVAHAEPTDINMEENDDDLPLSTLQNKIRRRKRRHTIRK*