AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034398

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034398 MtrunA17_Chr6g0485431 89.474 361 38 0 1 361 1 361 0.0 664
MsaG034398 MtrunA17_Chr1g0189391 45.075 335 152 7 58 361 1 334 5.58e-81 250
MsaG034398 MtrunA17_Chr7g0249311 43.089 123 68 2 103 225 342 462 3.77e-24 103
MsaG034398 MtrunA17_Chr7g0249311 40.741 81 46 2 155 235 180 258 1.32e-13 72.0
MsaG034398 MtrunA17_Chr5g0418191 50.000 98 40 2 140 237 306 394 1.06e-23 101
MsaG034398 MtrunA17_Chr5g0418191 39.450 109 55 5 126 230 119 220 1.07e-13 72.0
MsaG034398 MtrunA17_Chr7g0263531 34.868 152 94 3 80 231 291 437 3.49e-23 100
MsaG034398 MtrunA17_Chr7g0263531 46.667 75 38 2 155 229 190 262 5.85e-14 72.8
MsaG034398 MtrunA17_Chr4g0042831 56.000 75 32 1 140 214 264 337 1.45e-22 97.8
MsaG034398 MtrunA17_Chr4g0042831 47.059 68 34 2 148 214 138 204 5.47e-13 69.7
MsaG034398 MtrunA17_Chr7g0218081 45.833 96 51 1 119 214 375 469 1.67e-22 98.6
MsaG034398 MtrunA17_Chr7g0218081 48.387 62 30 2 153 214 227 286 3.26e-12 67.8
MsaG034398 MtrunA17_Chr3g0129321 35.606 132 77 3 84 215 55 178 3.66e-22 93.2
MsaG034398 MtrunA17_Chr7g0249301 55.263 76 33 1 139 214 277 351 1.01e-21 95.1
MsaG034398 MtrunA17_Chr7g0249301 40.964 83 47 2 153 235 61 141 3.60e-13 70.1
MsaG034398 MtrunA17_Chr3g0101131 37.179 156 82 5 74 214 320 474 1.11e-21 96.3
MsaG034398 MtrunA17_Chr5g0445311 34.211 152 96 2 63 214 276 423 1.17e-21 96.3
MsaG034398 MtrunA17_Chr4g0051881 49.383 81 40 1 140 220 164 243 1.34e-21 94.4
MsaG034398 MtrunA17_Chr7g0270191 43.182 88 49 1 141 228 132 218 1.75e-21 93.2
MsaG034398 MtrunA17_Chr1g0194481 46.835 79 41 1 140 218 144 221 6.78e-21 92.0
MsaG034398 MtrunA17_Chr5g0443131 48.148 81 41 1 140 220 143 222 1.38e-20 90.9
MsaG034398 MtrunA17_Chr3g0079691 45.631 103 55 1 112 214 453 554 5.98e-20 91.3
MsaG034398 MtrunA17_Chr3g0079691 46.154 65 33 2 155 219 281 343 7.36e-12 66.6
MsaG034398 MtrunA17_Chr4g0004911 52.632 76 35 1 140 215 322 396 1.07e-19 90.1
MsaG034398 MtrunA17_Chr4g0004911 44.615 65 35 1 155 219 185 248 2.91e-13 70.9
MsaG034398 MtrunA17_Chr2g0294231 44.444 81 44 1 140 220 189 268 1.86e-19 88.6
MsaG034398 MtrunA17_Chr3g0129361 48.611 72 36 1 140 211 130 200 1.91e-19 85.9
MsaG034398 MtrunA17_Chr8g0383351 47.945 73 36 2 140 211 145 216 4.06e-19 85.5
MsaG034398 MtrunA17_Chr5g0418781 48.718 78 36 2 140 214 110 186 5.11e-19 85.9
MsaG034398 MtrunA17_Chr4g0068541 44.872 78 42 1 141 218 195 271 6.26e-19 87.4
MsaG034398 MtrunA17_Chr7g0248721 50.685 73 34 2 139 211 126 196 8.82e-19 84.0
MsaG034398 MtrunA17_Chr7g0243891 53.333 75 34 1 140 214 360 433 1.73e-18 86.7
MsaG034398 MtrunA17_Chr7g0243891 38.750 80 47 2 150 229 212 289 6.80e-12 66.6
MsaG034398 MtrunA17_Chr8g0383251 46.753 77 40 1 141 217 95 170 1.78e-18 82.4
MsaG034398 MtrunA17_Chr5g0432161 48.193 83 42 1 132 214 399 480 1.87e-18 86.7
MsaG034398 MtrunA17_Chr5g0432161 43.750 80 43 2 152 231 242 319 5.72e-11 63.9
MsaG034398 MtrunA17_Chr5g0447701 35.714 126 80 1 89 214 187 311 2.37e-18 85.5
MsaG034398 MtrunA17_Chr3g0106491 52.000 75 35 1 140 214 351 424 3.44e-18 85.9
MsaG034398 MtrunA17_Chr3g0106491 42.105 76 42 2 145 220 189 262 2.44e-12 67.8
MsaG034398 MtrunA17_Chr8g0335811 47.222 72 37 1 140 211 140 210 7.28e-18 81.6
MsaG034398 MtrunA17_Chr7g0224801 50.000 68 33 1 144 211 83 149 1.66e-17 79.3
MsaG034398 MtrunA17_Chr1g0188941 46.667 75 39 1 140 214 37 110 2.58e-17 77.0
MsaG034398 MtrunA17_Chr1g0150801 47.297 74 38 1 138 211 86 158 2.61e-17 78.6
MsaG034398 MtrunA17_Chr8g0386241 46.479 71 37 1 141 211 90 159 3.97e-16 75.9
MsaG034398 MtrunA17_Chr3g0099641 40.517 116 55 5 134 248 232 334 7.77e-16 78.6
MsaG034398 MtrunA17_Chr4g0074001 45.000 80 43 1 140 219 345 423 1.31e-15 78.2
MsaG034398 MtrunA17_Chr7g0238921 34.921 126 72 3 87 211 64 180 1.55e-15 76.6
MsaG034398 MtrunA17_Chr4g0060271 46.053 76 39 2 141 215 242 316 4.44e-15 76.6
MsaG034398 MtrunA17_Chr2g0293481 39.048 105 59 4 155 258 268 368 7.09e-15 75.5
MsaG034398 MtrunA17_Chr2g0284321 51.613 62 28 2 154 214 35 95 8.45e-15 72.8
MsaG034398 MtrunA17_Chr7g0216891 30.846 201 108 7 49 232 105 291 8.79e-15 75.5
MsaG034398 MtrunA17_Chr8g0379461 49.206 63 30 2 154 215 76 137 1.02e-14 73.2
MsaG034398 MtrunA17_Chr7g0270831 46.667 75 38 2 141 214 203 276 1.24e-14 74.7
MsaG034398 MtrunA17_Chr5g0401821 34.821 112 60 3 110 220 166 265 1.38e-14 73.2
MsaG034398 MtrunA17_Chr8g0348311 47.561 82 38 4 147 226 147 225 1.52e-14 73.9
MsaG034398 MtrunA17_Chr1g0147881 49.254 67 32 2 155 220 263 328 2.08e-14 73.6
MsaG034398 MtrunA17_Chr2g0322391 46.667 75 38 2 141 214 336 409 3.56e-14 73.9
MsaG034398 MtrunA17_Chr2g0288871 45.333 75 39 2 141 214 292 365 3.57e-14 73.6
MsaG034398 MtrunA17_Chr2g0319961 50.794 63 29 2 154 215 80 141 3.61e-14 71.6
MsaG034398 MtrunA17_Chr7g0270051 46.250 80 35 3 142 214 35 113 3.83e-14 71.6
MsaG034398 MtrunA17_Chr7g0248691 50.000 78 35 3 150 226 150 224 5.98e-14 72.0
MsaG034398 MtrunA17_Chr4g0071521 47.059 68 34 2 151 217 242 308 6.18e-14 72.0
MsaG034398 MtrunA17_Chr3g0139021 39.806 103 49 3 110 211 281 371 9.24e-14 72.0
MsaG034398 MtrunA17_Chr1g0150281 39.773 88 47 4 130 212 112 198 1.51e-13 70.5
MsaG034398 MtrunA17_Chr4g0051181 45.000 80 42 2 137 215 226 304 1.61e-13 71.6
MsaG034398 MtrunA17_Chr8g0387501 39.583 96 48 4 155 249 130 216 2.07e-13 69.7
MsaG034398 MtrunA17_Chr2g0333851 35.789 95 57 3 141 232 154 247 2.51e-13 70.1
MsaG034398 MtrunA17_Chr2g0302391 46.753 77 39 2 155 230 212 287 2.65e-13 70.9
MsaG034398 MtrunA17_Chr4g0053281 35.714 98 50 3 117 213 240 325 3.68e-13 69.7
MsaG034398 MtrunA17_Chr4g0025061 42.500 80 44 2 141 219 347 425 5.48e-13 70.1
MsaG034398 MtrunA17_Chr8g0387981 43.750 80 36 3 138 209 111 189 6.91e-13 68.9
MsaG034398 MtrunA17_Chr7g0228941 51.724 58 26 2 155 211 53 109 1.62e-12 63.9
MsaG034398 MtrunA17_Chr1g0184691 50.847 59 27 2 155 212 294 351 1.63e-12 67.8
MsaG034398 MtrunA17_Chr1g0202461 47.945 73 34 3 155 226 262 331 5.24e-12 66.6
MsaG034398 MtrunA17_Chr1g0150271 33.684 95 59 3 123 214 68 161 9.87e-12 64.7
MsaG034398 MtrunA17_Chr5g0447711 40.000 75 44 1 140 214 216 289 8.55e-11 62.4
MsaG034398 MtrunA17_Chr3g0136961 40.984 61 34 2 153 212 137 196 9.22e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034398 AT5G41570.1 53.425 73 33 1 139 211 83 154 1.12e-22 94.0
MsaG034398 AT1G13960.1 54.667 75 33 1 140 214 395 468 1.65e-22 99.0
MsaG034398 AT1G13960.1 46.575 73 36 3 148 219 222 292 3.63e-13 70.9
MsaG034398 AT4G18170.1 56.000 75 32 1 140 214 158 231 1.76e-22 96.7
MsaG034398 AT1G29860.1 56.757 74 31 1 141 214 123 195 1.91e-22 95.9
MsaG034398 AT1G13960.2 54.667 75 33 1 140 214 242 315 2.55e-22 97.1
MsaG034398 AT1G13960.2 46.575 73 36 3 148 219 69 139 1.26e-12 68.6
MsaG034398 AT2G03340.1 54.667 75 33 1 140 214 401 474 3.32e-22 98.2
MsaG034398 AT2G03340.1 47.761 67 32 3 149 214 244 308 1.41e-12 69.3
MsaG034398 AT1G64000.1 53.521 71 32 1 141 211 101 170 3.52e-22 93.2
MsaG034398 AT5G46350.1 54.667 75 33 1 140 214 169 242 6.08e-22 95.5
MsaG034398 AT5G56270.1 38.194 144 85 3 71 214 407 546 9.31e-21 94.4
MsaG034398 AT5G56270.1 50.877 57 26 2 155 211 274 328 2.25e-11 65.9
MsaG034398 AT2G47260.1 47.436 78 40 1 141 218 161 237 1.76e-20 91.7
MsaG034398 AT3G01080.2 53.333 75 34 1 140 214 330 403 1.99e-20 92.8
MsaG034398 AT3G01080.2 48.387 62 30 2 153 214 204 263 4.93e-11 64.3
MsaG034398 AT1G69310.4 46.591 88 40 3 140 221 133 219 2.11e-20 90.5
MsaG034398 AT1G69310.2 46.591 88 40 3 140 221 133 219 2.11e-20 90.5
MsaG034398 AT1G69310.1 46.591 88 40 3 140 221 133 219 2.11e-20 90.5
MsaG034398 AT3G01080.1 53.333 75 34 1 140 214 292 365 2.40e-20 92.0
MsaG034398 AT3G01080.1 49.180 61 29 2 154 214 167 225 5.83e-11 63.9
MsaG034398 AT5G49520.1 49.333 75 37 1 141 215 208 281 2.44e-20 92.0
MsaG034398 AT2G46130.1 51.389 72 34 1 140 211 16 86 7.62e-20 84.3
MsaG034398 AT5G07100.1 54.667 75 33 1 140 214 220 293 9.16e-20 89.0
MsaG034398 AT5G07100.1 33.636 110 64 4 131 237 91 194 3.06e-11 64.3
MsaG034398 AT5G07100.2 54.667 75 33 1 140 214 127 200 9.31e-20 87.0
MsaG034398 AT5G07100.4 54.667 75 33 1 140 214 127 200 9.31e-20 87.0
MsaG034398 AT5G07100.5 54.667 75 33 1 140 214 127 200 9.31e-20 87.0
MsaG034398 AT5G07100.3 54.667 75 33 1 140 214 127 200 9.31e-20 87.0
MsaG034398 AT1G55600.1 50.000 74 36 1 141 214 294 366 1.76e-19 90.1
MsaG034398 AT5G64810.1 32.847 137 84 2 75 211 38 166 4.63e-19 84.7
MsaG034398 AT4G26640.7 52.000 75 35 1 140 214 327 400 4.82e-19 89.0
MsaG034398 AT4G26640.2 52.000 75 35 1 140 214 367 440 5.54e-19 88.6
MsaG034398 AT4G26640.1 52.000 75 35 1 140 214 295 368 6.24e-19 88.2
MsaG034398 AT4G26640.5 52.000 75 35 1 140 214 191 264 7.46e-19 87.4
MsaG034398 AT4G26640.6 52.000 75 35 1 140 214 191 264 7.46e-19 87.4
MsaG034398 AT4G26640.4 52.000 75 35 1 140 214 191 264 7.46e-19 87.4
MsaG034398 AT4G26640.3 52.000 75 35 1 140 214 191 264 7.46e-19 87.4
MsaG034398 AT2G04880.2 50.000 74 36 1 141 214 270 342 9.88e-19 87.8
MsaG034398 AT2G04880.2 40.217 92 51 3 148 237 105 194 2.71e-11 65.1
MsaG034398 AT2G38470.1 52.000 75 35 1 140 214 348 421 9.96e-19 87.8
MsaG034398 AT2G04880.1 50.000 74 36 1 141 214 294 366 1.13e-18 87.4
MsaG034398 AT2G04880.1 39.216 102 56 4 148 245 105 204 3.32e-11 64.7
MsaG034398 AT2G21900.1 44.156 77 39 2 143 216 95 170 1.16e-18 83.6
MsaG034398 AT2G37260.3 52.174 69 32 1 143 211 258 325 1.61e-18 85.9
MsaG034398 AT2G37260.3 36.220 127 56 5 155 281 86 187 2.10e-12 67.8
MsaG034398 AT4G39410.1 50.000 72 35 1 140 211 209 279 1.62e-18 85.5
MsaG034398 AT2G37260.2 52.174 69 32 1 143 211 256 323 1.70e-18 85.9
MsaG034398 AT2G37260.2 36.220 127 56 5 155 281 84 185 2.26e-12 67.8
MsaG034398 AT2G37260.1 54.688 64 28 1 148 211 343 405 1.91e-18 86.7
MsaG034398 AT2G37260.1 36.220 127 56 5 155 281 166 267 1.75e-12 68.6
MsaG034398 AT5G26170.1 45.070 71 38 1 141 211 100 169 2.10e-18 82.0
MsaG034398 AT2G30250.1 45.679 81 43 1 140 220 314 393 2.23e-18 86.3
MsaG034398 AT2G44745.1 47.222 72 37 1 140 211 131 201 3.12e-18 82.8
MsaG034398 AT3G62340.1 46.154 78 41 1 139 216 103 179 4.54e-18 83.6
MsaG034398 AT4G30935.1 48.718 78 39 1 155 232 332 408 1.52e-17 84.0
MsaG034398 AT5G13080.1 47.887 71 36 1 141 211 54 123 5.73e-17 77.4
MsaG034398 AT4G26440.1 40.404 99 58 1 116 214 334 431 6.04e-17 82.4
MsaG034398 AT4G23550.1 38.655 119 61 5 137 247 124 238 1.57e-16 79.7
MsaG034398 AT4G01250.1 42.991 107 47 5 154 247 128 233 5.75e-16 77.8
MsaG034398 AT1G69310.3 43.182 88 39 4 140 221 133 215 1.01e-15 77.0
MsaG034398 AT1G29280.1 49.231 65 31 2 154 217 74 137 1.74e-15 75.9
MsaG034398 AT2G46130.2 47.222 72 33 2 140 211 16 82 3.09e-15 71.2
MsaG034398 AT3G01970.1 41.667 72 41 1 141 212 52 122 1.12e-14 71.2
MsaG034398 AT1G30650.1 49.206 63 30 2 154 215 217 278 2.38e-14 74.3
MsaG034398 AT2G34830.1 49.254 67 30 3 155 220 216 279 2.47e-14 74.3
MsaG034398 AT2G34830.2 49.254 67 30 3 155 220 225 288 2.56e-14 74.3
MsaG034398 AT5G15130.2 46.053 76 39 2 141 215 172 246 2.76e-14 74.3
MsaG034398 AT1G62300.1 48.000 75 37 2 141 214 299 372 2.81e-14 74.3
MsaG034398 AT5G15130.1 46.053 76 39 2 141 215 214 288 3.03e-14 74.3
MsaG034398 AT1G68150.1 41.304 92 52 2 137 227 218 308 3.29e-14 73.6
MsaG034398 AT4G04450.2 46.667 75 38 2 141 214 185 258 3.67e-14 73.9
MsaG034398 AT3G58710.1 40.385 104 57 4 154 254 71 172 3.93e-14 72.4
MsaG034398 AT3G58710.2 40.385 104 57 4 154 254 70 171 4.08e-14 72.0
MsaG034398 AT2G25000.2 46.032 63 32 2 153 214 102 163 4.76e-14 71.2
MsaG034398 AT2G25000.4 46.032 63 32 2 153 214 102 163 4.76e-14 71.2
MsaG034398 AT4G01720.1 48.000 75 37 2 141 214 226 299 4.87e-14 73.6
MsaG034398 AT2G30590.1 34.000 150 81 6 79 211 222 370 7.16e-14 72.8
MsaG034398 AT2G25000.3 46.032 63 32 2 153 214 124 185 8.25e-14 70.9
MsaG034398 AT2G25000.1 46.032 63 32 2 153 214 145 206 1.13e-13 70.9
MsaG034398 AT4G22070.4 46.667 75 38 2 141 214 284 357 1.28e-13 72.4
MsaG034398 AT4G22070.3 46.667 75 38 2 141 214 284 357 1.28e-13 72.4
MsaG034398 AT4G22070.2 46.667 75 38 2 141 214 284 357 1.28e-13 72.4
MsaG034398 AT4G22070.1 46.667 75 38 2 141 214 284 357 1.28e-13 72.4
MsaG034398 AT4G04450.1 46.667 75 38 2 141 214 279 352 1.30e-13 72.4
MsaG034398 AT4G31550.2 39.216 102 49 3 111 211 213 302 1.39e-13 71.2
MsaG034398 AT2G23320.1 40.541 111 42 5 112 213 204 299 1.74e-13 70.9
MsaG034398 AT4G31550.1 53.448 58 25 2 155 211 247 303 3.32e-13 70.1
MsaG034398 AT1G18860.1 43.529 85 45 3 131 214 217 299 3.88e-13 70.9
MsaG034398 AT4G24240.1 36.697 109 62 4 115 217 237 344 4.03e-13 70.1
MsaG034398 AT5G52830.1 42.308 104 49 4 150 252 161 254 5.02e-13 69.7
MsaG034398 AT5G43290.1 40.000 85 49 2 153 236 113 196 5.50e-13 68.9
MsaG034398 AT4G31800.2 40.000 80 44 3 153 229 110 188 8.78e-13 67.8
MsaG034398 AT5G28650.1 38.235 102 50 3 111 211 230 319 1.25e-12 68.6
MsaG034398 AT4G31800.1 40.000 80 44 3 153 229 175 253 1.37e-12 68.2
MsaG034398 AT4G11070.2 31.250 144 79 6 126 251 76 217 3.76e-12 66.6
MsaG034398 AT4G11070.1 31.250 144 79 6 126 251 108 249 5.81e-12 66.2
MsaG034398 AT2G24570.1 51.724 58 26 2 155 211 244 300 5.92e-12 66.2
MsaG034398 AT3G04670.1 38.235 102 50 3 111 211 230 319 6.05e-12 66.6
MsaG034398 AT1G80840.1 41.270 63 35 2 153 214 145 206 6.38e-12 66.2
MsaG034398 AT5G45050.4 46.875 64 32 2 154 216 1041 1103 9.89e-12 67.0
MsaG034398 AT5G45050.1 46.875 64 32 2 154 216 1180 1242 1.05e-11 67.0
MsaG034398 AT5G45050.2 46.875 64 32 2 154 216 1152 1214 1.06e-11 67.0
MsaG034398 AT5G24110.1 48.333 60 27 2 153 209 112 170 1.43e-11 65.1

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAACTTGTTGTTCATCTA+AGG 0.247246 6:-111778532 None:intergenic
GGGAAGGAAATATTTGAAAT+TGG 0.258518 6:-111778336 None:intergenic
CATCAGCTGCAACAACTTCT+TGG 0.283729 6:-111778494 None:intergenic
TATGAAACAATTACATATTT+CGG 0.286298 6:-111779634 None:intergenic
ATGCCTTATGGATATATTAA+TGG 0.289062 6:+111779895 MsaT034398.1:CDS
TATTTGAAATCCACCAATAC+TGG 0.290258 6:-111779974 None:intergenic
TTCAAGAAAGGATTATTATT+AGG 0.304221 6:-111779850 None:intergenic
TCAATTCTAAACACTACTTT+TGG 0.313871 6:-111778806 None:intergenic
CGAAGAAAATCATGAAGTTC+TGG 0.347870 6:-111780009 None:intergenic
AATACCATGATGATGCCTTA+TGG 0.355278 6:+111779883 MsaT034398.1:CDS
TCTGAAAGTTATGAATCTTA+TGG 0.371782 6:+111779811 MsaT034398.1:CDS
GGTTATCGTTGGCATAAGTA+TGG 0.380213 6:+111778854 MsaT034398.1:CDS
CCTAAATATCATTCATTACT+AGG 0.380869 6:-111780114 None:intergenic
TCGTTGGCTTCATGATTAAT+TGG 0.383142 6:-111780087 None:intergenic
AGTGTAGTACTTGACTTTGT+TGG 0.385433 6:-111779703 None:intergenic
TATGTAATTGTTTCATATGA+AGG 0.393620 6:+111779640 MsaT034398.1:CDS
CAAGTTACAAATACAACTAT+TGG 0.398555 6:+111778546 MsaT034398.1:CDS
AAGCAACATCATTCCTTCAA+TGG 0.416911 6:-111778583 None:intergenic
TTGGATGTTGAACTTCATCT+TGG 0.424960 6:-111778317 None:intergenic
TGTGAGAAAACAGGTGGAAA+GGG 0.431043 6:+111779600 MsaT034398.1:CDS
GCATCTTCACGATAATTCAT+TGG 0.435145 6:-111778465 None:intergenic
GATGGTTTCCACATGGCAAC+GGG 0.443847 6:+111778279 None:intergenic
CAAGCTGTTCCCAGTATTGG+TGG 0.453491 6:+111779964 MsaT034398.1:CDS
ATCAGCTGCAACAACTTCTT+GGG 0.455443 6:-111778493 None:intergenic
TCTCAAGCTGTTCCCAGTAT+TGG 0.455665 6:+111779961 MsaT034398.1:CDS
GGTGAGGAGGTGTGAAAGGT+TGG 0.471015 6:-111779786 None:intergenic
GGTGTGAAAGGTTGGGTCAT+AGG 0.474536 6:-111779778 None:intergenic
TTCCACATGGCAACGGGAGG+AGG 0.481747 6:+111778285 None:intergenic
TTATTGACCATGTTCAAGAA+AGG 0.487320 6:-111779862 None:intergenic
ATTGGTGCAGCACCTTCAAA+AGG 0.488078 6:-111780069 None:intergenic
GTCCTTGATCAAGTGTTGTT+GGG 0.488952 6:-111778356 None:intergenic
GCTTGAGAGGCAGCAATTGA+AGG 0.494366 6:-111779946 None:intergenic
CTTATGGTAAGAACTGTACT+TGG 0.502973 6:+111779827 MsaT034398.1:CDS
TTCCCAACAACACTTGATCA+AGG 0.508819 6:+111778354 MsaT034398.1:CDS
GTTGAAGCAAGTTCCATTGA+AGG 0.510335 6:+111778570 MsaT034398.1:CDS
GCTAAACGCTGAAATTCTAA+AGG 0.517982 6:+111778425 MsaT034398.1:CDS
CATAATGATACTAGTGCTAG+TGG 0.520978 6:+111778609 MsaT034398.1:CDS
AACCTGTAATGTGAGAAAAC+AGG 0.528589 6:+111779591 MsaT034398.1:CDS
AATGAAGACAAAGTGAATGA+TGG 0.529985 6:+111778833 MsaT034398.1:CDS
TTGATCAAGTGTTGTTGGGA+AGG 0.530594 6:-111778352 None:intergenic
GTGAGGAGGTGTGAAAGGTT+GGG 0.531143 6:-111779785 None:intergenic
ATGTGAGAAAACAGGTGGAA+AGG 0.535021 6:+111779599 MsaT034398.1:CDS
TAATAATCCTTTCTTGAACA+TGG 0.535267 6:+111779855 MsaT034398.1:CDS
AAATCATGAAGTTCTGGTAG+TGG 0.536148 6:-111780003 None:intergenic
TGATGGTTTCCACATGGCAA+CGG 0.539953 6:+111778278 None:intergenic
TGTCCATTAATATATCCATA+AGG 0.552023 6:-111779898 None:intergenic
TGTCCTTGATCAAGTGTTGT+TGG 0.552454 6:-111778357 None:intergenic
AATACTGGGAACAGCTTGAG+AGG 0.558169 6:-111779959 None:intergenic
TCATGAAGTTCTGGTAGTGG+TGG 0.567685 6:-111780000 None:intergenic
GAGCGGTGAGGAGGTGTGAA+AGG 0.568345 6:-111779790 None:intergenic
TTCATTACTAGGACTGTCGT+TGG 0.572830 6:-111780103 None:intergenic
TGGGTCATAGGCAATGCAAA+AGG 0.578767 6:-111779766 None:intergenic
AAGGAAATGCTAAATACCCT+AGG 0.580382 6:+111778891 MsaT034398.1:CDS
ATTTGAAATCCACCAATACT+GGG 0.584480 6:-111779973 None:intergenic
GCAACATTTATCATGGTGCA+AGG 0.584736 6:-111778782 None:intergenic
GAAATTCTAAAGGCTAGGAA+AGG 0.587867 6:+111778435 MsaT034398.1:CDS
ACGCTGAAATTCTAAAGGCT+AGG 0.591343 6:+111778430 MsaT034398.1:CDS
TAATTGCATTACTTTGCACA+TGG 0.597072 6:-111778401 None:intergenic
TATGGACGCAAGGAGATCAA+AGG 0.600725 6:+111778872 MsaT034398.1:CDS
TGCATTATAGGGGATACTAC+AGG 0.601431 6:+111779557 MsaT034398.1:intron
GCTGCAACAACTTCTTGGGT+AGG 0.614907 6:-111778489 None:intergenic
TTGGCATAAGTATGGACGCA+AGG 0.617304 6:+111778862 MsaT034398.1:CDS
CTAGTGGATCTTCTTCACAA+AGG 0.623228 6:+111778625 MsaT034398.1:CDS
TAACTTTCAGAGCGGTGAGG+AGG 0.625278 6:-111779799 None:intergenic
TATGAAGGCAAACATAATCA+CGG 0.626212 6:+111779655 MsaT034398.1:CDS
AAGTGAATGATGGTTATCGT+TGG 0.626452 6:+111778843 MsaT034398.1:CDS
GTCATAGGCAATGCAAAAGG+TGG 0.645286 6:-111779763 None:intergenic
CTGTAATGTGAGAAAACAGG+TGG 0.647364 6:+111779594 MsaT034398.1:CDS
CAACATTTATCATGGTGCAA+GGG 0.652471 6:-111778781 None:intergenic
ATATCCATAAGGCATCATCA+TGG 0.655849 6:-111779887 None:intergenic
GGTTTCCACATGGCAACGGG+AGG 0.656503 6:+111778282 None:intergenic
ACAACACTTGATCAAGGACA+TGG 0.685805 6:+111778360 MsaT034398.1:CDS
TCATAACTTTCAGAGCGGTG+AGG 0.687777 6:-111779802 None:intergenic
AAGATTCATAACTTTCAGAG+CGG 0.728852 6:-111779807 None:intergenic
CACTGACAACAGTAGCACGT+AGG 0.750828 6:+111779722 MsaT034398.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 111778291 111780128 111778291 ID=MsaG034398
Chr6 mRNA 111778291 111780128 111778291 ID=MsaT034398.1;Parent=MsaG034398
Chr6 exon 111778291 111778646 111778291 ID=MsaT034398.1.exon1;Parent=MsaT034398.1
Chr6 CDS 111778291 111778646 111778291 ID=cds.MsaT034398.1;Parent=MsaT034398.1
Chr6 exon 111778745 111778912 111778745 ID=MsaT034398.1.exon2;Parent=MsaT034398.1
Chr6 CDS 111778745 111778912 111778745 ID=cds.MsaT034398.1;Parent=MsaT034398.1
Chr6 exon 111779567 111780128 111779567 ID=MsaT034398.1.exon3;Parent=MsaT034398.1
Chr6 CDS 111779567 111780128 111779567 ID=cds.MsaT034398.1;Parent=MsaT034398.1
Gene Sequence

>MsaT034398.1

ATGGCAACGGGAGGAGGTGTAGAACTCCAAGATGAAGTTCAACATCCAATTTCAAATATTTCCTTCCCAACAACACTTGATCAAGGACATGGTGCAGAAAAAGAACAAGTCCATGTGCAAAGTAATGCAATTACGCTAAACGCTGAAATTCTAAAGGCTAGGAAAGGAAAGATGCCAATGAATTATCGTGAAGATGCACCTACCCAAGAAGTTGTTGCAGCTGATGAAGCAAAAACGGTATCCTTAGATGAACAACAAGTTACAAATACAACTATTGGTGTTGAAGCAAGTTCCATTGAAGGAATGATGTTGCTTCAACATAATGATACTAGTGCTAGTGGATCTTCTTCACAAAGGAAGAGGAAAATTGACTTCTCTGAACACTATAAACTCCCTTGCACCATGATAAATGTTGCGCCAAAAGTAGTGTTTAGAATTGAAATCAATGAAGACAAAGTGAATGATGGTTATCGTTGGCATAAGTATGGACGCAAGGAGATCAAAGGAAATGCTAAATACCCTAGGGGATACTACAGGTGTACAACAAAAACCTGTAATGTGAGAAAACAGGTGGAAAGGGATGCACAGAATCCGAAATATGTAATTGTTTCATATGAAGGCAAACATAATCACGGATTCCCTATCATAACAAAAAAAAATCCAACAAAGTCAAGTACTACACTGACAACAGTAGCACGTAGGAATGCTAGCATTGTATCGCCACCTTTTGCATTGCCTATGACCCAACCTTTCACACCTCCTCACCGCTCTGAAAGTTATGAATCTTATGGTAAGAACTGTACTTGGCCTAATAATAATCCTTTCTTGAACATGGTCAATAATACCATGATGATGCCTTATGGATATATTAATGGACAACATTTTCGAGATTGTTATGCTGCTCCTTCAATTGCTGCCTCTCAAGCTGTTCCCAGTATTGGTGGATTTCAAATATCTCCACCACTACCAGAACTTCATGATTTTCTTCGACTTAATGGGTACAATATTGCTGCTGCTAGAATGTTTCCTTTTGAAGGTGCTGCACCAATTAATCATGAAGCCAACGACAGTCCTAGTAATGAATGA
Protein sequence

>MsaT034398.1

MATGGGVELQDEVQHPISNISFPTTLDQGHGAEKEQVHVQSNAITLNAEILKARKGKMPMNYREDAPTQEVVAADEAKTVSLDEQQVTNTTIGVEASSIEGMMLLQHNDTSASGSSSQRKRKIDFSEHYKLPCTMINVAPKVVFRIEINEDKVNDGYRWHKYGRKEIKGNAKYPRGYYRCTTKTCNVRKQVERDAQNPKYVIVSYEGKHNHGFPIITKKNPTKSSTTLTTVARRNASIVSPPFALPMTQPFTPPHRSESYESYGKNCTWPNNNPFLNMVNNTMMMPYGYINGQHFRDCYAAPSIAASQAVPSIGGFQISPPLPELHDFLRLNGYNIAAARMFPFEGAAPINHEANDSPSNE*