AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034399

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034399 MtrunA17_Chr6g0485441 99.499 798 4 0 1 798 1 798 0.0 1621
MsaG034399 MtrunA17_Chr1g0189451 75.714 210 45 2 587 796 1 204 2.35e-91 285
MsaG034399 MtrunA17_Chr1g0189431 87.417 151 19 0 1 151 1 151 3.47e-90 281
MsaG034399 MtrunA17_Chr4g0013741 22.954 562 367 14 58 575 96 635 1.57e-25 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034399 AT2G40730.1 78.625 800 164 5 1 796 1 797 0.0 1212
MsaG034399 AT1G71410.1 20.940 468 309 10 58 483 96 544 1.12e-18 91.7
MsaG034399 AT1G71410.2 20.940 468 309 10 58 483 96 544 1.12e-18 91.7
MsaG034399 AT1G22870.4 21.165 515 344 10 58 530 96 590 1.26e-18 91.7
MsaG034399 AT1G22870.1 21.165 515 344 10 58 530 96 590 1.76e-18 91.3
MsaG034399 AT1G22870.3 21.698 424 299 7 133 530 9 425 2.05e-17 87.4

Find 227 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAACCTTGGTGGAATTT+TGG 0.064969 6:-111795272 None:intergenic
TTAGCTTCTGCCCTTGAATT+TGG 0.180640 6:+111788511 MsaT034399.1:CDS
ATCCGCTGAGCACCTAATTT+TGG 0.188714 6:-111795481 None:intergenic
TTTGACTGCATTGTTGAAAA+TGG 0.188813 6:+111788552 MsaT034399.1:CDS
CTCCATGGGCCATTGATTCT+TGG 0.193517 6:+111786344 MsaT034399.1:CDS
TGAAGAAATGACAAAATATT+TGG 0.211866 6:-111784966 None:intergenic
ATCCACCAACAACTTCTTTC+AGG 0.230681 6:-111784430 None:intergenic
TTATCTGAAAGTGGCATTAC+AGG 0.240664 6:-111785053 None:intergenic
GGAAGCTGCTCTGCTAAATT+TGG 0.248475 6:-111788356 None:intergenic
ACTTGATTATTCAGGCATTA+TGG 0.255952 6:+111790604 MsaT034399.1:intron
CCAAGTAAACTTGCATTTCC+TGG 0.262803 6:-111792127 None:intergenic
AAACCTGCTGCTCCAAAATT+AGG 0.271088 6:+111795469 MsaT034399.1:CDS
CTGTCTCAAAGGACCTTGTC+TGG 0.286397 6:+111789921 MsaT034399.1:CDS
CCAGGAAATGCAAGTTTACT+TGG 0.288202 6:+111792127 MsaT034399.1:CDS
TCCATGGGCCATTGATTCTT+GGG 0.291032 6:+111786345 MsaT034399.1:CDS
GTTGTCGAATTCAAATTTAA+AGG 0.302494 6:-111795355 None:intergenic
CTGAGTAGCCGCTGGTAATC+TGG 0.310087 6:-111787415 None:intergenic
CATTGTTGAAAATGGGTTCC+TGG 0.312737 6:+111788560 MsaT034399.1:CDS
TCTCAAGTCCATGTTAGTTC+TGG 0.320181 6:+111789003 MsaT034399.1:CDS
ACCCTTCCGCTTGGGGCTCT+TGG 0.325303 6:+111784498 MsaT034399.1:CDS
CTTTCTATTATAGACAAATA+TGG 0.328878 6:+111792063 MsaT034399.1:intron
GCCACTTTCAGATAAGATTC+AGG 0.330183 6:+111785061 MsaT034399.1:CDS
CTTAGCAGCTGGCCGAGGTC+GGG 0.342150 6:+111795441 MsaT034399.1:CDS
AAACAGTGATGTTCGATCTA+AGG 0.342598 6:+111791335 MsaT034399.1:intron
CTTTGTCTATTGCAGGTTCA+TGG 0.345575 6:+111785752 MsaT034399.1:intron
TCAGATAAGATTCAGGAGTT+AGG 0.347337 6:+111785068 MsaT034399.1:CDS
CGAACCTTACCCTTCCGCTT+GGG 0.349078 6:+111784490 MsaT034399.1:CDS
AAAGAAGTTGTTGGTGGATC+TGG 0.357725 6:+111784434 MsaT034399.1:CDS
GATGGAAGTTTATCAATTCC+AGG 0.362464 6:+111792109 MsaT034399.1:CDS
AGCTCAAGCTGTTGATGAAC+AGG 0.365464 6:+111788795 MsaT034399.1:CDS
CCGACCTCGGCCAGCTGCTA+AGG 0.373064 6:-111795440 None:intergenic
CAGCTCCTGCGCCCACTACA+AGG 0.373259 6:+111795410 MsaT034399.1:CDS
CTCCTACATCAACGGATGGC+TGG 0.376358 6:+111794645 MsaT034399.1:CDS
CTAATCTATGAGCTGTTCTC+TGG 0.378549 6:+111787185 MsaT034399.1:CDS
TCCTACATCAACGGATGGCT+GGG 0.381579 6:+111794646 MsaT034399.1:CDS
GTTTACCCTCATGTTGCTAC+TGG 0.382745 6:+111788944 MsaT034399.1:CDS
AATTTCATTGATGTCATAGT+TGG 0.383892 6:-111790645 None:intergenic
GGTAGACACAATACATTTCA+TGG 0.388081 6:+111788280 MsaT034399.1:CDS
GCCATTGATTCTTGGGGAAT+GGG 0.389642 6:+111786352 MsaT034399.1:CDS
TAAGGTTCGCCGATGTTGTA+TGG 0.391413 6:-111784476 None:intergenic
AAAGCATCTATCGAAGTTAC+AGG 0.393427 6:+111789953 MsaT034399.1:CDS
CGGATAAACCGGACATCATC+AGG 0.394990 6:+111795499 MsaT034399.1:CDS
CTCCGAATTGTTCAATATGT+TGG 0.396831 6:-111788762 None:intergenic
CAGGGATGAATATTATGCTT+GGG 0.398038 6:+111785599 MsaT034399.1:intron
ACAAATATGGCAGAGACTAC+TGG 0.399143 6:+111792076 MsaT034399.1:CDS
ATTGTGAGTACAACAACATT+AGG 0.400000 6:-111790683 None:intergenic
GCAAAGAGTTTAATTATTGT+TGG 0.401711 6:-111788706 None:intergenic
CGGTGTTAATTTCAGGATGA+TGG 0.402052 6:+111784602 MsaT034399.1:intron
CTCCAACATATTGAACAATT+CGG 0.407638 6:+111788760 MsaT034399.1:CDS
TTTAAAGGTCTTGATGTTGT+TGG 0.410922 6:-111795340 None:intergenic
TCAAGGGCAGAAGCTAATAA+AGG 0.423515 6:-111788505 None:intergenic
TTCAACAATGCAGTCAAAGC+AGG 0.425072 6:-111788547 None:intergenic
TCGGCCAGCTGCTAAGGGCC+TGG 0.432686 6:-111795434 None:intergenic
GCATGGTGGTCAGTGAAATC+TGG 0.432933 6:-111794614 None:intergenic
TTAGCAGCTGGCCGAGGTCG+GGG 0.437675 6:+111795442 MsaT034399.1:CDS
GATGTTCTATCAGAGTTTGA+TGG 0.438526 6:+111785836 MsaT034399.1:CDS
AGGGCCAGGCCCTTAGCAGC+TGG 0.442542 6:+111795430 MsaT034399.1:CDS
CTGGCCCTTGTAGTGGGCGC+AGG 0.445959 6:-111795415 None:intergenic
GGCCATTGATTCTTGGGGAA+TGG 0.447612 6:+111786351 MsaT034399.1:CDS
GACACGGACAGCTCGATCAT+TGG 0.450921 6:-111788731 None:intergenic
CTAGAACTCACAGAAGCAAC+TGG 0.451326 6:-111793513 None:intergenic
TCTTCATCCCTGCTCAACCT+TGG 0.453545 6:-111795283 None:intergenic
CCTTAGCAGCTGGCCGAGGT+CGG 0.453563 6:+111795440 MsaT034399.1:CDS
AAATTCCACCAAGGTTGAGC+AGG 0.454216 6:+111795275 MsaT034399.1:CDS
ATGAGCTCCTTGACTCTAAA+GGG 0.459180 6:+111793471 MsaT034399.1:CDS
TAGGTGCTCAGCGGATAAAC+CGG 0.460603 6:+111795488 MsaT034399.1:CDS
CCGCAGCTAAATGTCCATCC+TGG 0.462680 6:-111784664 None:intergenic
AGGCCCTTAGCAGCTGGCCG+AGG 0.465336 6:+111795436 MsaT034399.1:CDS
TGCAATATTTCCTAGCAAGA+TGG 0.466669 6:-111790289 None:intergenic
TTTGAAAGATAGTGTTGAGA+AGG 0.467964 6:+111788313 MsaT034399.1:CDS
ATTGATGTCATAGTTGGAAC+TGG 0.469305 6:-111790639 None:intergenic
ATTCAGGAGTTAGGTCTTGA+AGG 0.470304 6:+111785077 MsaT034399.1:CDS
TGTTGTTGTCACGCCAACTT+TGG 0.470386 6:+111785793 MsaT034399.1:CDS
GCGAACCTTACCCTTCCGCT+TGG 0.471772 6:+111784489 MsaT034399.1:CDS
CATTAGAAGATGTTGGTGTA+AGG 0.476759 6:-111793539 None:intergenic
AAGGGCCTGGCCCTTGTAGT+GGG 0.480173 6:-111795421 None:intergenic
ATGTTCTATCAGAGTTTGAT+GGG 0.482527 6:+111785837 MsaT034399.1:CDS
GTTGTTGGTGGATCTGGAAC+TGG 0.482994 6:+111784440 MsaT034399.1:CDS
CTGCGCCCACTACAAGGGCC+AGG 0.483015 6:+111795416 MsaT034399.1:CDS
TTGACTGCATTGTTGAAAAT+GGG 0.485246 6:+111788553 MsaT034399.1:CDS
CAAATAATAAATCACCTGTC+AGG 0.485509 6:-111790710 None:intergenic
GGTTCATGGCAATGTTTGCT+TGG 0.485983 6:+111785766 MsaT034399.1:intron
TCCTGAATCTTATCTGAAAG+TGG 0.486236 6:-111785062 None:intergenic
CAACCTGTTTCACAGACAAA+AGG 0.486565 6:+111794803 MsaT034399.1:CDS
CGACCTCGGCCAGCTGCTAA+GGG 0.488359 6:-111795439 None:intergenic
GTTGGCTTTGTTTCTTCAAG+TGG 0.490626 6:-111794734 None:intergenic
CATTTAGCTGCGGGTCGTAA+TGG 0.491773 6:+111784674 MsaT034399.1:CDS
TATGAGCTCCTTGACTCTAA+AGG 0.491816 6:+111793470 MsaT034399.1:CDS
ACATCCCTCCTGATGATGTC+CGG 0.492711 6:-111795507 None:intergenic
GGTCTTGATGTTGTTGGAGC+GGG 0.492740 6:-111795334 None:intergenic
TAAGGGCCTGGCCCTTGTAG+TGG 0.492890 6:-111795422 None:intergenic
CAGCAGCTGAACCAAATTCA+AGG 0.493179 6:-111788522 None:intergenic
TTGTCACGCCAACTTTGGAC+TGG 0.493752 6:+111785798 MsaT034399.1:CDS
TCGTCAGATTGTGTTAAAGA+AGG 0.495220 6:+111788379 MsaT034399.1:CDS
GAGCCCCAAGCGGAAGGGTA+AGG 0.495780 6:-111784494 None:intergenic
GGGATGAAGATGAAGATTTG+TGG 0.496658 6:+111795296 MsaT034399.1:CDS
CAAATATGGCAGAGACTACT+GGG 0.496846 6:+111792077 MsaT034399.1:CDS
ATAATTGATTGTTACCTTGG+AGG 0.496898 6:-111784537 None:intergenic
GCAGGGATGAATATTATGCT+TGG 0.497318 6:+111785598 MsaT034399.1:intron
CAACTAATGATGATGATCCA+TGG 0.500400 6:+111795377 MsaT034399.1:CDS
ACCCATTCCCCAAGAATCAA+TGG 0.501604 6:-111786353 None:intergenic
TCATCCCTGCTCAACCTTGG+TGG 0.502638 6:-111795280 None:intergenic
TGTGAAACAGGTTGAACAAC+TGG 0.506071 6:-111794794 None:intergenic
TTTCCACCTGCTAGAGGAGC+AGG 0.506104 6:+111790489 MsaT034399.1:CDS
TCCGTTGATGTAGGAGATGT+AGG 0.506786 6:-111794638 None:intergenic
GGAGCTGCAATGGCAGCCCA+TGG 0.507768 6:-111795394 None:intergenic
CTAATACCTGCTCCTCTAGC+AGG 0.508317 6:-111790495 None:intergenic
GAACTCCTCAGCTGAAAGCC+AGG 0.510223 6:-111788578 None:intergenic
GAGAAGTCTGACCTTGGGAA+TGG 0.510862 6:-111787249 None:intergenic
GCTTGAATGTTAGTAAGAGC+TGG 0.513816 6:-111794758 None:intergenic
GAACCTTACCCTTCCGCTTG+GGG 0.515759 6:+111784491 MsaT034399.1:CDS
AAATTCCTGAAAGAAGTTGT+TGG 0.517801 6:+111784425 MsaT034399.1:CDS
TGGAGTTTCCAGTCCAAAGT+TGG 0.518208 6:-111785806 None:intergenic
CGGAGCTAGCAAAATCCGAC+TGG 0.519464 6:+111786302 MsaT034399.1:CDS
CAGGTTTGGCTCCCCGACCT+CGG 0.519595 6:-111795453 None:intergenic
CAGGATGGACATTTAGCTGC+GGG 0.519763 6:+111784665 MsaT034399.1:CDS
GGATGAAGATGAAGATTTGT+GGG 0.520644 6:+111795297 MsaT034399.1:CDS
CCTACATCAACGGATGGCTG+GGG 0.521802 6:+111794647 MsaT034399.1:CDS
CCGTTGATGTAGGAGATGTA+GGG 0.523241 6:-111794637 None:intergenic
ATCATTAAGCATACCTGAGC+TGG 0.523577 6:-111793562 None:intergenic
TACTCACAATTGATCCTGAC+AGG 0.528935 6:+111790696 MsaT034399.1:CDS
GACATCCTCTACGCAAATGC+TGG 0.529946 6:+111785868 MsaT034399.1:CDS
AGCCAAGAGCCCCAAGCGGA+AGG 0.530407 6:-111784500 None:intergenic
CGCAGCTAAATGTCCATCCT+GGG 0.530668 6:-111784663 None:intergenic
CCATGGGCCATTGATTCTTG+GGG 0.532546 6:+111786346 MsaT034399.1:CDS
CAGGAAATGCAAGTTTACTT+GGG 0.535552 6:+111792128 MsaT034399.1:CDS
GGGTTCCTGGCTTTCAGCTG+AGG 0.537536 6:+111788573 MsaT034399.1:CDS
TAAATGTCCATCCTGGGCAT+TGG 0.537721 6:-111784657 None:intergenic
TTCCTGAAAGAAGTTGTTGG+TGG 0.541191 6:+111784428 MsaT034399.1:CDS
GATGTATTTAATCTACGAGA+AGG 0.543062 6:-111787445 None:intergenic
TCAACAATGCAGTCAAAGCA+GGG 0.543212 6:-111788546 None:intergenic
ACACGGATAGGTGCTGTTGA+AGG 0.546664 6:-111794581 None:intergenic
AGCTGAGGAGTTCCGTGTCA+AGG 0.546667 6:+111788588 MsaT034399.1:CDS
ACAAATACAACCATCTTGCT+AGG 0.548057 6:+111790279 MsaT034399.1:CDS
TGTTGGAGCGGGAGCTGCTA+TGG 0.549412 6:-111795323 None:intergenic
AGGCTTGCCCTTTAGAGTCA+AGG 0.549491 6:-111793478 None:intergenic
CATGTTAGTTCTGGCTCCGA+AGG 0.549605 6:+111789012 MsaT034399.1:CDS
GCCAAGAGCCCCAAGCGGAA+GGG 0.551717 6:-111784499 None:intergenic
AGTGGGCGCAGGAGCTGCAA+TGG 0.552530 6:-111795404 None:intergenic
ATTGTTCAATATGTTGGAGA+AGG 0.552863 6:-111788756 None:intergenic
CTGCTAAATTTGGAAGCTTG+CGG 0.553281 6:-111788346 None:intergenic
ATCATTATTTAAGAAGCTCA+CGG 0.554036 6:-111785643 None:intergenic
TGCTCCTCTAGCAGGTGGAA+AGG 0.555846 6:-111790487 None:intergenic
AAGGTTCGCCGATGTTGTAT+GGG 0.556759 6:-111784475 None:intergenic
CTGATAGAACATCAAAAGCA+TGG 0.560392 6:-111785826 None:intergenic
GCAACTGGAGCATGATCAGA+AGG 0.561662 6:-111793498 None:intergenic
AGCTCCTGCGCCCACTACAA+GGG 0.562278 6:+111795411 MsaT034399.1:CDS
ATTGCAAGCTACTTAAACGA+AGG 0.562326 6:+111790306 MsaT034399.1:CDS
CTACATCAACGGATGGCTGG+GGG 0.562918 6:+111794648 MsaT034399.1:CDS
TTGCAAGCTACTTAAACGAA+GGG 0.564838 6:+111790307 MsaT034399.1:CDS
CATAATTGCAGCTGTCTCAA+AGG 0.565208 6:+111789910 MsaT034399.1:intron
ACATCTTCTAATGCCAGCTC+AGG 0.570241 6:+111793549 MsaT034399.1:CDS
CTTCGGAGCCAGAACTAACA+TGG 0.570780 6:-111789011 None:intergenic
CCCTACATCTCCTACATCAA+CGG 0.571117 6:+111794637 MsaT034399.1:CDS
TAATTAAGAAGTCTCCTCCA+TGG 0.572719 6:+111786329 MsaT034399.1:CDS
CTTCTTAATTACAGCCCAGT+CGG 0.573033 6:-111786317 None:intergenic
ATGTAGGAGATGTAGGGGCA+TGG 0.573984 6:-111794631 None:intergenic
ATGTTAGTAAGAGCTGGAGT+TGG 0.578383 6:-111794752 None:intergenic
ATTAGAAGATGTTGGTGTAA+GGG 0.578476 6:-111793538 None:intergenic
TGTCTCAAAGGACCTTGTCT+GGG 0.579463 6:+111789922 MsaT034399.1:CDS
CTGAAAATGACAAGGATGGG+TGG 0.581469 6:+111794699 MsaT034399.1:CDS
TCATCATCGCGGTACCTCCA+AGG 0.585096 6:+111784523 MsaT034399.1:CDS
AAGTCTGACCTTGGGAATGG+AGG 0.585606 6:-111787246 None:intergenic
AACCTGAAAATGACAAGGAT+GGG 0.586228 6:+111794696 MsaT034399.1:CDS
ATTATCTGGAACCAATGCCC+AGG 0.586298 6:+111784646 MsaT034399.1:CDS
CCCCAGCCATCCGTTGATGT+AGG 0.587906 6:-111794647 None:intergenic
GCGTGGAACTGAGTAGCCGC+TGG 0.590412 6:-111787423 None:intergenic
AACTAATGATGATGATCCAT+GGG 0.594078 6:+111795378 MsaT034399.1:CDS
TCTGGAACCAATGCCCAGGA+TGG 0.595289 6:+111784650 MsaT034399.1:CDS
TTTCCATTCAGCAATATGCA+TGG 0.596821 6:+111786254 MsaT034399.1:intron
AATTAAGAAGTCTCCTCCAT+GGG 0.608185 6:+111786330 MsaT034399.1:CDS
AGGGATGAATATTATGCTTG+GGG 0.608296 6:+111785600 MsaT034399.1:intron
CCCCAAGAATCAATGGCCCA+TGG 0.613929 6:-111786346 None:intergenic
AATTCCACCAAGGTTGAGCA+GGG 0.616045 6:+111795276 MsaT034399.1:CDS
AATTATTAGTTACCTTGACA+CGG 0.618397 6:-111788600 None:intergenic
CTTTAATAGTGACCCAGACA+AGG 0.619287 6:-111789934 None:intergenic
GATACCTTTCCACCTGCTAG+AGG 0.619442 6:+111790483 MsaT034399.1:CDS
AGCAGCTGAACCAAATTCAA+GGG 0.620239 6:-111788521 None:intergenic
AATTACCAGCATTTGCGTAG+AGG 0.620808 6:-111785873 None:intergenic
GAACCTGAAAATGACAAGGA+TGG 0.621311 6:+111794695 MsaT034399.1:CDS
TATGTTGGAGAAGGCTGACA+CGG 0.621891 6:-111788747 None:intergenic
ATACCTGCTCCTCTAGCAGG+TGG 0.623925 6:-111790492 None:intergenic
ACATTCAAGCAGCTCAAAGA+CGG 0.624681 6:+111794771 MsaT034399.1:CDS
GGTGTGGAATTTACACGGAT+AGG 0.624750 6:-111794593 None:intergenic
ACGGACAGCTCGATCATTGG+AGG 0.625109 6:-111788728 None:intergenic
ATTTAATCTACGAGAAGGCG+TGG 0.626292 6:-111787440 None:intergenic
CTCTGCTTCCAGATTACCAG+CGG 0.627746 6:+111787407 MsaT034399.1:CDS
AGGTCTTGATGTTGTTGGAG+CGG 0.630097 6:-111795335 None:intergenic
GAGAAACCAGTAGCAACATG+AGG 0.635595 6:-111788950 None:intergenic
GCTAGAGAATGGAATTGATG+AGG 0.638647 6:+111794673 MsaT034399.1:CDS
TACATCAACGGATGGCTGGG+GGG 0.641241 6:+111794649 MsaT034399.1:CDS
CAACAATGCAGTCAAAGCAG+GGG 0.641805 6:-111788545 None:intergenic
TTCTTTAACACAATCTGACG+AGG 0.643601 6:-111788377 None:intergenic
GGCTCTTGGCTTCATCATCG+CGG 0.646469 6:+111784512 MsaT034399.1:CDS
TGAAAATGACAAGGATGGGT+GGG 0.647528 6:+111794700 MsaT034399.1:CDS
TGCAACACAATACAAATCAA+CGG 0.647901 6:+111786282 MsaT034399.1:CDS
ACATCTCCTACATCAACGGA+TGG 0.648116 6:+111794641 MsaT034399.1:CDS
TAACACTGCCTCCATTCCCA+AGG 0.650706 6:+111787238 MsaT034399.1:CDS
GGCAAAGCAACACTACGAGA+AGG 0.650955 6:+111791389 MsaT034399.1:CDS
CGTTGATGTAGGAGATGTAG+GGG 0.651141 6:-111794636 None:intergenic
GAAAGGTATCACGTAAAGCA+CGG 0.652734 6:-111790470 None:intergenic
AATCTGGTGTGGAATTTACA+CGG 0.654113 6:-111794598 None:intergenic
AGAAACCAGTAGCAACATGA+GGG 0.659703 6:-111788949 None:intergenic
CCAGGATGGACATTTAGCTG+CGG 0.659961 6:+111784664 MsaT034399.1:CDS
GTGGTCAGTGAAATCTGGTG+TGG 0.662798 6:-111794609 None:intergenic
CTCCAAAATTAGGTGCTCAG+CGG 0.663729 6:+111795479 MsaT034399.1:CDS
GGAGCTAGCAAAATCCGACT+GGG 0.664324 6:+111786303 MsaT034399.1:CDS
ACATCAATGAAATTGCCACT+CGG 0.673599 6:+111790654 MsaT034399.1:CDS
TGCAAGCTACTTAAACGAAG+GGG 0.676042 6:+111790308 MsaT034399.1:CDS
GAGCTGGCATTAGAAGATGT+TGG 0.677571 6:-111793546 None:intergenic
TAGGAGATGTAGGGGCATGG+TGG 0.678043 6:-111794628 None:intergenic
CGCGACCCAAAATTCCACCA+AGG 0.678187 6:+111795266 MsaT034399.1:CDS
AGAGATCTCCCATACAACAT+CGG 0.678627 6:+111784467 MsaT034399.1:CDS
TTTGAAGTTGAGCAAAACAG+AGG 0.685379 6:+111787208 MsaT034399.1:CDS
ATAAACCGGACATCATCAGG+AGG 0.688981 6:+111795502 MsaT034399.1:CDS
TCAAAGCTTATAGAAAACAG+TGG 0.696006 6:+111787466 MsaT034399.1:CDS
TAAACCGGACATCATCAGGA+GGG 0.696522 6:+111795503 MsaT034399.1:CDS
TGTTGTATGGGAGATCTCTG+AGG 0.714551 6:-111784463 None:intergenic
ATGAAGCCAAGAGCCCCAAG+CGG 0.722990 6:-111784504 None:intergenic
AACATTAGGAAGAATCCGAG+TGG 0.723039 6:-111790669 None:intergenic
GATGTCATAGTTGGAACTGG+TGG 0.723344 6:-111790636 None:intergenic
TAGGTCTTGAAGGTACACAA+AGG 0.723793 6:+111785087 MsaT034399.1:CDS
CAAGAATCAATGGCCCATGG+AGG 0.724818 6:-111786343 None:intergenic
TGAGGAACCTGAAAATGACA+AGG 0.725485 6:+111794691 MsaT034399.1:CDS
AAATATGGCAGAGACTACTG+GGG 0.748805 6:+111792078 MsaT034399.1:CDS
GATGAAGATGAAGATTTGTG+GGG 0.753066 6:+111795298 MsaT034399.1:CDS
AATATGGCAGAGACTACTGG+GGG 0.765761 6:+111792079 MsaT034399.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 111784419 111795531 111784419 ID=MsaG034399
Chr6 mRNA 111784419 111795531 111784419 ID=MsaT034399.1;Parent=MsaG034399
Chr6 exon 111784419 111784544 111784419 ID=MsaT034399.1.exon1;Parent=MsaT034399.1
Chr6 CDS 111784419 111784544 111784419 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111784617 111784715 111784617 ID=MsaT034399.1.exon2;Parent=MsaT034399.1
Chr6 CDS 111784617 111784715 111784617 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111784957 111785108 111784957 ID=MsaT034399.1.exon3;Parent=MsaT034399.1
Chr6 CDS 111784957 111785108 111784957 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111785602 111785674 111785602 ID=MsaT034399.1.exon4;Parent=MsaT034399.1
Chr6 CDS 111785602 111785674 111785602 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111785767 111785889 111785767 ID=MsaT034399.1.exon5;Parent=MsaT034399.1
Chr6 CDS 111785767 111785889 111785767 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111786265 111786373 111786265 ID=MsaT034399.1.exon6;Parent=MsaT034399.1
Chr6 CDS 111786265 111786373 111786265 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111787180 111787259 111787180 ID=MsaT034399.1.exon7;Parent=MsaT034399.1
Chr6 CDS 111787180 111787259 111787180 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111787406 111787487 111787406 ID=MsaT034399.1.exon8;Parent=MsaT034399.1
Chr6 CDS 111787406 111787487 111787406 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111788261 111788400 111788261 ID=MsaT034399.1.exon9;Parent=MsaT034399.1
Chr6 CDS 111788261 111788400 111788261 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111788499 111788609 111788499 ID=MsaT034399.1.exon10;Parent=MsaT034399.1
Chr6 CDS 111788499 111788609 111788499 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111788700 111788816 111788700 ID=MsaT034399.1.exon11;Parent=MsaT034399.1
Chr6 CDS 111788700 111788816 111788700 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111788944 111789033 111788944 ID=MsaT034399.1.exon12;Parent=MsaT034399.1
Chr6 CDS 111788944 111789033 111788944 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111789921 111789974 111789921 ID=MsaT034399.1.exon13;Parent=MsaT034399.1
Chr6 CDS 111789921 111789974 111789921 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111790255 111790329 111790255 ID=MsaT034399.1.exon14;Parent=MsaT034399.1
Chr6 CDS 111790255 111790329 111790255 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111790435 111790510 111790435 ID=MsaT034399.1.exon15;Parent=MsaT034399.1
Chr6 CDS 111790435 111790510 111790435 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111790618 111790717 111790618 ID=MsaT034399.1.exon16;Parent=MsaT034399.1
Chr6 CDS 111790618 111790717 111790618 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111791341 111791410 111791341 ID=MsaT034399.1.exon17;Parent=MsaT034399.1
Chr6 CDS 111791341 111791410 111791341 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111792076 111792149 111792076 ID=MsaT034399.1.exon18;Parent=MsaT034399.1
Chr6 CDS 111792076 111792149 111792076 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111793464 111793570 111793464 ID=MsaT034399.1.exon19;Parent=MsaT034399.1
Chr6 CDS 111793464 111793570 111793464 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111794561 111794824 111794561 ID=MsaT034399.1.exon20;Parent=MsaT034399.1
Chr6 CDS 111794561 111794824 111794561 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Chr6 exon 111795257 111795531 111795257 ID=MsaT034399.1.exon21;Parent=MsaT034399.1
Chr6 CDS 111795257 111795531 111795257 ID=cds.MsaT034399.1;Parent=MsaT034399.1
Gene Sequence

>MsaT034399.1

ATGTTCAAATTCCTGAAAGAAGTTGTTGGTGGATCTGGAACTGGCCTCAGAGATCTCCCATACAACATCGGCGAACCTTACCCTTCCGCTTGGGGCTCTTGGCTTCATCATCGCGGTACCTCCAAGGATGATGGTTCACCAGTTTCAATTTTTTCATTATCTGGAACCAATGCCCAGGATGGACATTTAGCTGCGGGTCGTAATGGTGTCAAGCGTCTACGAACTGTTAGGCATCCAAATATTTTGTCATTTCTTCATAGTTCTGAGATTGAAACTTTTGATGGCGGTTCTTCTAAGGTTACAATTTATATTGTGACTGAACCTGTAATGCCACTTTCAGATAAGATTCAGGAGTTAGGTCTTGAAGGTACACAAAGGGATGAATATTATGCTTGGGGACTGCTTCAAATAGCGAAAGCCGTGAGCTTCTTAAATAATGATTGTAAACTTGTTCATGGCAATGTTTGCTTGGCTAGTGTTGTTGTCACGCCAACTTTGGACTGGAAACTCCATGCTTTTGATGTTCTATCAGAGTTTGATGGGAGTAATGAGACATCCTCTACGCAAATGCTGCAATATGCATGGCTTGTTGCAACACAATACAAATCAACGGAGCTAGCAAAATCCGACTGGGCTGTAATTAAGAAGTCTCCTCCATGGGCCATTGATTCTTGGGGAATGGGTTGTCTAATCTATGAGCTGTTCTCTGGTTTGAAGTTGAGCAAAACAGAGGACTTACGTAACACTGCCTCCATTCCCAAGTCTCTGCTTCCAGATTACCAGCGGCTACTCAGTTCCACGCCTTCTCGTAGATTAAATACATCAAAGCTTATAGAAAACAGTGAGTATTTTCAGAATAAGTTGGTAGACACAATACATTTCATGGAAATTCTTAGTTTGAAAGATAGTGTTGAGAAGGACACTTTCTTCCGCAAGCTTCCAAATTTAGCAGAGCAGCTTCCTCGTCAGATTGTGTTAAAGAAGTTACTTCCTTTATTAGCTTCTGCCCTTGAATTTGGTTCAGCTGCTGCCCCTGCTTTGACTGCATTGTTGAAAATGGGTTCCTGGCTTTCAGCTGAGGAGTTCCGTGTCAAGGTTCTTCCAACAATAATTAAACTCTTTGCCTCCAATGATCGAGCTGTCCGTGTCAGCCTTCTCCAACATATTGAACAATTCGGAGAGTCATTATCAGCTCAAGCTGTTGATGAACAGGTTTACCCTCATGTTGCTACTGGTTTCTCTGATACATCTGCTTTTTTGAGGGAACTTACTCTCAAGTCCATGTTAGTTCTGGCTCCGAAGCTGTCTCAAAGGACCTTGTCTGGGTCACTATTAAAGCATCTATCGAAGTTACAGGTCGATGAAGAGGCTGCGATACGAACAAATACAACCATCTTGCTAGGAAATATTGCAAGCTACTTAAACGAAGGGACAAGAAAAAGAGTGTTGATTAATGCATTTACAGTCCGTGCTTTACGTGATACCTTTCCACCTGCTAGAGGAGCAGGCATTATGGCTTTATGTGCCACCAGTTCCAACTATGACATCAATGAAATTGCCACTCGGATTCTTCCTAATGTTGTTGTACTCACAATTGATCCTGACAGTGATGTTCGATCTAAGGCATTTCAAGCTATTGATCAATTTTTGCAAATGGCAAAGCAACACTACGAGAAGACAAATATGGCAGAGACTACTGGGGGTGCAAGCGATGGAAGTTTATCAATTCCAGGAAATGCAAGTTTACTTGGATGGGCTATGAGCTCCTTGACTCTAAAGGGCAAGCCTTCTGATCATGCTCCAGTTGCTTCTGTGAGTTCTAGTGCCCTTACACCAACATCTTCTAATGCCAGCTCAGGCATTCTAGTTGCAGACACTCCTTCAACAGCACCTATCCGTGTAAATTCCACACCAGATTTCACTGACCACCATGCCCCTACATCTCCTACATCAACGGATGGCTGGGGGGAGCTAGAGAATGGAATTGATGAGGAACCTGAAAATGACAAGGATGGGTGGGATGATTTAGAACCACTTGAAGAAACAAAGCCAACTCCAGCTCTTACTAACATTCAAGCAGCTCAAAGACGGCCAGTTGTTCAACCTGTTTCACAGACAAAAGCTTCAAGTTCGCGACCCAAAATTCCACCAAGGTTGAGCAGGGATGAAGATGAAGATTTGTGGGGTGCCATAGCAGCTCCCGCTCCAACAACATCAAGACCTTTAAATTTGAATTCGACAACAACTAATGATGATGATCCATGGGCTGCCATTGCAGCTCCTGCGCCCACTACAAGGGCCAGGCCCTTAGCAGCTGGCCGAGGTCGGGGAGCCAAACCTGCTGCTCCAAAATTAGGTGCTCAGCGGATAAACCGGACATCATCAGGAGGGATGTAA
Protein sequence

>MsaT034399.1

MFKFLKEVVGGSGTGLRDLPYNIGEPYPSAWGSWLHHRGTSKDDGSPVSIFSLSGTNAQDGHLAAGRNGVKRLRTVRHPNILSFLHSSEIETFDGGSSKVTIYIVTEPVMPLSDKIQELGLEGTQRDEYYAWGLLQIAKAVSFLNNDCKLVHGNVCLASVVVTPTLDWKLHAFDVLSEFDGSNETSSTQMLQYAWLVATQYKSTELAKSDWAVIKKSPPWAIDSWGMGCLIYELFSGLKLSKTEDLRNTASIPKSLLPDYQRLLSSTPSRRLNTSKLIENSEYFQNKLVDTIHFMEILSLKDSVEKDTFFRKLPNLAEQLPRQIVLKKLLPLLASALEFGSAAAPALTALLKMGSWLSAEEFRVKVLPTIIKLFASNDRAVRVSLLQHIEQFGESLSAQAVDEQVYPHVATGFSDTSAFLRELTLKSMLVLAPKLSQRTLSGSLLKHLSKLQVDEEAAIRTNTTILLGNIASYLNEGTRKRVLINAFTVRALRDTFPPARGAGIMALCATSSNYDINEIATRILPNVVVLTIDPDSDVRSKAFQAIDQFLQMAKQHYEKTNMAETTGGASDGSLSIPGNASLLGWAMSSLTLKGKPSDHAPVASVSSSALTPTSSNASSGILVADTPSTAPIRVNSTPDFTDHHAPTSPTSTDGWGELENGIDEEPENDKDGWDDLEPLEETKPTPALTNIQAAQRRPVVQPVSQTKASSSRPKIPPRLSRDEDEDLWGAIAAPAPTTSRPLNLNSTTTNDDDPWAAIAAPAPTTRARPLAAGRGRGAKPAAPKLGAQRINRTSSGGM*