AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034415


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034415 MtrunA17_Chr6g0485571 82.051 351 30 4 1 324 1 345 0.0 513
MsaG034415 MtrunA17_Chr5g0410881 34.298 242 117 6 3 207 24 260 4.28e-35 133
MsaG034415 MtrunA17_Chr3g0135831 33.333 204 109 2 11 187 46 249 8.24e-31 119
MsaG034415 MtrunA17_Chr1g0204741 31.646 237 130 5 11 218 40 273 9.92e-30 115
MsaG034415 MtrunA17_Chr7g0260331 31.602 231 123 5 11 208 49 277 2.03e-29 115
MsaG034415 MtrunA17_Chr4g0039891 33.019 212 107 5 6 182 7 218 8.89e-29 115
MsaG034415 MtrunA17_Chr4g0071051 34.946 186 101 3 11 176 101 286 3.76e-26 107
MsaG034415 MtrunA17_Chr6g0467041 33.654 208 110 5 9 188 20 227 7.56e-24 99.8
MsaG034415 MtrunA17_Chr8g0375041 61.765 68 26 0 3 70 1 68 2.46e-23 96.7
MsaG034415 MtrunA17_Chr8g0392001 35.204 196 93 4 9 170 20 215 4.16e-23 98.2
MsaG034415 MtrunA17_Chr8g0379701 31.034 203 105 5 9 182 10 206 2.38e-22 96.7
MsaG034415 MtrunA17_Chr8g0378061 32.093 215 104 5 12 191 23 230 1.68e-20 92.0
MsaG034415 MtrunA17_Chr2g0330821 30.102 196 100 3 10 175 78 266 1.78e-20 90.9
MsaG034415 MtrunA17_Chr1g0203601 56.522 69 30 0 2 70 9 77 2.08e-20 89.7
MsaG034415 MtrunA17_Chr5g0402291 55.882 68 30 0 3 70 1 68 1.29e-19 87.4
MsaG034415 MtrunA17_Chr5g0397881 54.545 66 30 0 5 70 18 83 1.61e-19 88.2
MsaG034415 MtrunA17_Chr5g0441531 57.576 66 28 0 5 70 19 84 1.61e-19 86.3
MsaG034415 MtrunA17_Chr2g0284481 31.892 185 88 3 9 159 13 193 2.06e-19 87.8
MsaG034415 MtrunA17_Chr3g0134031 58.730 63 26 0 8 70 21 83 4.88e-19 86.7
MsaG034415 MtrunA17_Chr1g0185131 29.911 224 102 5 11 196 32 238 7.04e-19 85.9
MsaG034415 MtrunA17_Chr7g0257331 54.098 61 28 0 10 70 21 81 1.08e-18 83.6
MsaG034415 MtrunA17_Chr1g0179321 51.429 70 34 0 1 70 39 108 2.82e-18 79.0
MsaG034415 MtrunA17_Chr6g0472421 51.515 66 32 0 5 70 27 92 5.97e-17 80.5
MsaG034415 MtrunA17_Chr4g0047421 49.231 65 29 1 10 70 32 96 2.17e-14 71.2
MsaG034415 MtrunA17_Chr4g0023771 50.000 62 31 0 7 68 11 72 5.94e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034415 AT3G24520.1 48.113 212 58 4 8 189 14 203 1.41e-54 181
MsaG034415 AT4G17750.1 36.777 242 104 6 6 203 47 283 2.71e-34 131
MsaG034415 AT1G32330.2 36.406 217 97 6 6 181 32 248 2.67e-32 125
MsaG034415 AT1G32330.1 34.562 217 101 4 6 181 32 248 2.99e-32 125
MsaG034415 AT3G51910.1 32.692 208 115 5 7 193 25 228 5.23e-30 115
MsaG034415 AT2G26150.1 34.146 205 104 4 11 184 44 248 1.85e-29 115
MsaG034415 AT2G26150.4 34.146 205 104 4 11 184 44 248 1.85e-29 115
MsaG034415 AT3G22830.1 30.677 251 135 7 11 224 61 309 4.48e-29 115
MsaG034415 AT3G63350.1 34.359 195 100 5 10 177 27 220 6.42e-28 110
MsaG034415 AT3G02990.1 34.615 208 102 4 9 182 21 228 1.26e-27 112
MsaG034415 AT5G16820.1 33.962 212 104 5 7 182 23 234 3.34e-27 111
MsaG034415 AT5G16820.2 33.962 212 104 5 7 182 23 234 3.34e-27 111
MsaG034415 AT5G03720.2 31.250 224 117 5 5 191 49 272 4.42e-27 110
MsaG034415 AT5G03720.1 31.250 224 117 5 5 191 49 272 4.42e-27 110
MsaG034415 AT1G67970.1 30.729 192 101 5 9 170 17 206 4.11e-25 104
MsaG034415 AT5G54070.1 30.270 185 107 4 8 172 68 250 2.22e-24 101
MsaG034415 AT5G43840.1 31.214 173 98 4 10 165 18 186 9.26e-21 90.9
MsaG034415 AT1G46264.1 58.730 63 26 0 8 70 31 93 3.68e-19 87.4
MsaG034415 AT4G13980.1 30.256 195 112 4 12 182 24 218 4.78e-19 87.8
MsaG034415 AT5G45710.3 28.472 288 161 8 3 251 1 282 4.88e-19 87.0
MsaG034415 AT5G45710.1 28.472 288 161 8 3 251 1 282 4.88e-19 87.0
MsaG034415 AT4G18880.1 29.730 259 144 4 9 232 13 268 6.51e-19 87.0
MsaG034415 AT4G11660.1 56.061 66 29 0 5 70 53 118 1.02e-18 86.3
MsaG034415 AT4G36990.1 51.515 66 32 0 5 70 8 73 2.72e-18 84.0
MsaG034415 AT2G41690.1 56.667 60 26 0 11 70 40 99 3.79e-17 80.1
MsaG034415 AT5G62020.1 51.515 66 32 0 5 70 17 82 1.04e-16 79.7

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAAGTGATAGTAGTAAAA+AGG 0.204760 6:+112135697 MsaT034415.1:CDS
AAGAGAGTGAGAGAATTCTA+AGG 0.267094 6:-112134560 None:intergenic
TGGCTCCGGTGGGTGGTTGG+TGG 0.288941 6:+112135573 MsaT034415.1:CDS
GCTCCGGTGGGTGGTTGGTG+GGG 0.299967 6:+112135575 MsaT034415.1:CDS
CCACCTTCTAACAACGAAAA+AGG 0.302300 6:-112135776 None:intergenic
AAGAAACATGTTGGTGGTAT+TGG 0.321921 6:+112135053 MsaT034415.1:CDS
TTGATAAGGTTATCTGTGTT+TGG 0.326130 6:-112134499 None:intergenic
AGTTTGGACAGATGGGTTAT+AGG 0.339452 6:+112135606 MsaT034415.1:CDS
CTCCGGTGGGTGGTTGGTGG+GGG 0.345456 6:+112135576 MsaT034415.1:CDS
ATTTGTTGAAGAACATAGTT+AGG 0.347369 6:+112135030 MsaT034415.1:intron
GGGCATGGCGGTGGCAAATT+CGG 0.364204 6:+112135439 MsaT034415.1:CDS
ACGATGAAACTGTTGTTAGA+TGG 0.404226 6:-112134529 None:intergenic
GGCTCCGGTGGGTGGTTGGT+GGG 0.407249 6:+112135574 MsaT034415.1:CDS
CGGCGCCGGCGCCGATGATG+TGG 0.410102 6:+112135666 MsaT034415.1:CDS
GCCGGTGTTGTCTCCGGCGC+CGG 0.429564 6:+112135652 MsaT034415.1:CDS
CATGTTGGTGGTATTGGAAA+TGG 0.433048 6:+112135059 MsaT034415.1:CDS
TTCGGTGAAGATGGAGTTTG+AGG 0.446034 6:+112135457 MsaT034415.1:CDS
AGTATTCTAGACAAAATTTG+AGG 0.453954 6:-112135305 None:intergenic
TGTCGGAAATTTCAATGAAA+TGG 0.454809 6:+112135724 MsaT034415.1:CDS
GTCAACTTCCTCGGAGTCAT+CGG 0.460070 6:+112135418 MsaT034415.1:CDS
TAGATGGGCCCCACTTGATA+AGG 0.462941 6:-112134513 None:intergenic
CCACCTCCTGCGGTGGTGCA+CGG 0.463396 6:-112135553 None:intergenic
ATGTCGTCCTCGCCGGAGCT+TGG 0.468090 6:+112135509 MsaT034415.1:CDS
CTTGTGTTCTTAAATGGGAA+TGG 0.469203 6:+112134420 None:intergenic
TTTAGAAGAAGATGAAGCTA+TGG 0.473347 6:+112135148 MsaT034415.1:CDS
TTGTTGTGTTTGAAGAAAAG+AGG 0.474502 6:-112134586 None:intergenic
GGAGTCATCGGGCATGGCGG+TGG 0.474769 6:+112135430 MsaT034415.1:CDS
CACATCATCGGCGCCGGCGC+CGG 0.474975 6:-112135665 None:intergenic
CAGGAGGTGGCTCCGGTGGG+TGG 0.478583 6:+112135566 MsaT034415.1:CDS
ACCGCAGGAGGTGGCTCCGG+TGG 0.478779 6:+112135562 MsaT034415.1:CDS
GGCGCCGGCGCCGATGATGT+GGG 0.479475 6:+112135667 MsaT034415.1:CDS
GTTGATATCTCCGGTGGCTA+AGG 0.482556 6:+112135373 MsaT034415.1:CDS
GGTGGCTAAGGCGGCCGTGA+CGG 0.483148 6:+112135385 MsaT034415.1:CDS
CCGTGCACCACCGCAGGAGG+TGG 0.485061 6:+112135553 MsaT034415.1:CDS
ACCACCGCAGGAGGTGGCTC+CGG 0.485869 6:+112135559 MsaT034415.1:CDS
ACGGATGCCGGTGTTGTCTC+CGG 0.489094 6:+112135646 MsaT034415.1:CDS
AGGTGGCTCCGGTGGGTGGT+TGG 0.489246 6:+112135570 MsaT034415.1:CDS
AATTTAGAAGAAGAGCTTCA+AGG 0.492990 6:+112135206 MsaT034415.1:CDS
GCAGATGGAGTTTGGACAGA+TGG 0.497763 6:+112135598 MsaT034415.1:CDS
CCGCAGGAGGTGGCTCCGGT+GGG 0.505617 6:+112135563 MsaT034415.1:CDS
TCAACTTCCTCGGAGTCATC+GGG 0.508311 6:+112135419 MsaT034415.1:CDS
CGATGAAACTGTTGTTAGAT+GGG 0.511534 6:-112134528 None:intergenic
TGACGTCGTTGCCGCCGTCA+CGG 0.512284 6:-112135399 None:intergenic
AGAAGATGAAGCTATGGTGA+TGG 0.518088 6:+112135154 MsaT034415.1:CDS
GGCAACGACGTCAACTTCCT+CGG 0.518118 6:+112135409 MsaT034415.1:CDS
CAGATGGAGTTTGGACAGAT+GGG 0.518140 6:+112135599 MsaT034415.1:CDS
GAAAAGACCACAACAAATGA+TGG 0.518696 6:+112135256 MsaT034415.1:CDS
GGCTAAGGCGGCCGTGACGG+CGG 0.526324 6:+112135388 MsaT034415.1:CDS
GAGGAAGATGAGGTAACGGT+TGG 0.526402 6:+112135476 MsaT034415.1:CDS
GATGGAGTTTGAGGAAGATG+AGG 0.529799 6:+112135466 MsaT034415.1:CDS
ACCGGAGCCACCTCCTGCGG+TGG 0.537226 6:-112135560 None:intergenic
TAACATGATGTCGTCCTCGC+CGG 0.538658 6:+112135502 MsaT034415.1:CDS
TTCCTCGGAGTCATCGGGCA+TGG 0.539288 6:+112135424 MsaT034415.1:CDS
GAAACGACGGTTGATATCTC+CGG 0.543586 6:+112135364 MsaT034415.1:CDS
GGTGGCAAATTCGGTGAAGA+TGG 0.545610 6:+112135448 MsaT034415.1:CDS
GTCACGGCCGCCTTAGCCAC+CGG 0.545636 6:-112135383 None:intergenic
TCAATTTCAAAACCAAGCTC+CGG 0.549174 6:-112135521 None:intergenic
TCACCCCACATCATCGGCGC+CGG 0.555196 6:-112135671 None:intergenic
GATATCTCCGGTGGCTAAGG+CGG 0.568045 6:+112135376 MsaT034415.1:CDS
TTGTGAAGTTCCACGGATGC+CGG 0.574030 6:+112135634 MsaT034415.1:CDS
CTATTCTCACCCCACATCAT+CGG 0.575525 6:-112135677 None:intergenic
TTTGCACCGTGCACCACCGC+AGG 0.578267 6:+112135547 MsaT034415.1:CDS
AGAGAGTGAGAGAATTCTAA+GGG 0.578397 6:-112134559 None:intergenic
GGAGACAACACCGGCATCCG+TGG 0.579394 6:-112135644 None:intergenic
GCACCGTGCACCACCGCAGG+AGG 0.584699 6:+112135550 MsaT034415.1:CDS
GGCGTGATTGTGAAGTTCCA+CGG 0.595018 6:+112135627 MsaT034415.1:CDS
CCCACCGGAGCCACCTCCTG+CGG 0.595130 6:-112135563 None:intergenic
GTTTGAGGAAGATGAGGTAA+CGG 0.595921 6:+112135472 MsaT034415.1:CDS
ACACAGATAACCTTATCAAG+TGG 0.597268 6:+112134503 MsaT034415.1:CDS
CACAGATAACCTTATCAAGT+GGG 0.597725 6:+112134504 MsaT034415.1:CDS
GCCGGCGCCGGAGACAACAC+CGG 0.598769 6:-112135653 None:intergenic
GCGCCGGCGCCGATGATGTG+GGG 0.609629 6:+112135668 MsaT034415.1:CDS
TTCAAAACCAAGCTCCGGCG+AGG 0.611782 6:-112135516 None:intergenic
GTTAGGAAGAAACATGTTGG+TGG 0.621782 6:+112135047 MsaT034415.1:CDS
CGCCATGCCCGATGACTCCG+AGG 0.624450 6:-112135426 None:intergenic
AAATTTCAATGAAATGGCGG+CGG 0.625567 6:+112135730 MsaT034415.1:CDS
CATTATGAAGACATACCACA+TGG 0.631169 6:+112134468 MsaT034415.1:CDS
TGTTTGGTTCATTAACCATG+TGG 0.639928 6:-112134483 None:intergenic
CGGAAATTTCAATGAAATGG+CGG 0.648772 6:+112135727 MsaT034415.1:CDS
ATAGTTAGGAAGAAACATGT+TGG 0.649558 6:+112135044 MsaT034415.1:CDS
ACGACGGTTGATATCTCCGG+TGG 0.680566 6:+112135367 MsaT034415.1:CDS
CTCGGAGTCATCGGGCATGG+CGG 0.681310 6:+112135427 MsaT034415.1:CDS
TGGTATGTCTTCATAATGAA+CGG 0.681423 6:-112134463 None:intergenic
TTGTTGATAACAAGAAACGA+CGG 0.686402 6:+112135351 MsaT034415.1:CDS
ACAGATAACCTTATCAAGTG+GGG 0.700643 6:+112134505 MsaT034415.1:CDS
TTGGTGGTATTGGAAATGGA+AGG 0.715173 6:+112135063 MsaT034415.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 112134433 112135805 112134433 ID=MsaG034415
Chr6 mRNA 112134433 112135805 112134433 ID=MsaT034415.1;Parent=MsaG034415
Chr6 exon 112134433 112134642 112134433 ID=MsaT034415.1.exon1;Parent=MsaT034415.1
Chr6 CDS 112134433 112134642 112134433 ID=cds.MsaT034415.1;Parent=MsaT034415.1
Chr6 exon 112135041 112135805 112135041 ID=MsaT034415.1.exon2;Parent=MsaT034415.1
Chr6 CDS 112135041 112135805 112135041 ID=cds.MsaT034415.1;Parent=MsaT034415.1
Gene Sequence

>MsaT034415.1

ATGGGAATGGAGCAAAACAACGTGATTGCACCGTTCATTATGAAGACATACCACATGGTTAATGAACCAAACACAGATAACCTTATCAAGTGGGGCCCATCTAACAACAGTTTCATCGTTCTTGATCCCTTAGAATTCTCTCACTCTCTTCTTCCTCTTTTCTTCAAACACAACAATTTCTCAAGCTTTGTTCGTCAACTCAACACTTATAACATAGTTAGGAAGAAACATGTTGGTGGTATTGGAAATGGAAGGAATAATAATAATAATAATTATTATAACAATAATAACTATTTTAATGAGGTGAAGTTTGAAGATTTAGAAGAAGATGAAGCTATGGTGATGGAAATAGCAAAGTTGAAAGAAGAACAAAGAAATTTAGAAGAAGAGCTTCAAGGAATGAACAAAAGATTAGAAACAACTGAGAAAAGACCACAACAAATGATGGCTTTTTTATGTAATGTCGTTGAAGATCCTCAAATTTTGTCTAGAATACTAAATGAAAGAGAGAGTAAAAAATTTGTTGATAACAAGAAACGACGGTTGATATCTCCGGTGGCTAAGGCGGCCGTGACGGCGGCAACGACGTCAACTTCCTCGGAGTCATCGGGCATGGCGGTGGCAAATTCGGTGAAGATGGAGTTTGAGGAAGATGAGGTAACGGTTGGTAATAACATGATGTCGTCCTCGCCGGAGCTTGGTTTTGAAATTGATTATTTGCACCGTGCACCACCGCAGGAGGTGGCTCCGGTGGGTGGTTGGTGGGGGCAGATGGAGTTTGGACAGATGGGTTATAGGCGTGATTGTGAAGTTCCACGGATGCCGGTGTTGTCTCCGGCGCCGGCGCCGATGATGTGGGGTGAGAATAGAAGTGATAGTAGTAAAAAGGGGGGTGTCGGAAATTTCAATGAAATGGCGGCGGAAAATAGTAAGTTGCCACCTTATCCTTTTTCGTTGTTAGAAGGTGGCTTTTAG

Protein sequence

>MsaT034415.1

MGMEQNNVIAPFIMKTYHMVNEPNTDNLIKWGPSNNSFIVLDPLEFSHSLLPLFFKHNNFSSFVRQLNTYNIVRKKHVGGIGNGRNNNNNNYYNNNNYFNEVKFEDLEEDEAMVMEIAKLKEEQRNLEEELQGMNKRLETTEKRPQQMMAFLCNVVEDPQILSRILNERESKKFVDNKKRRLISPVAKAAVTAATTSTSSESSGMAVANSVKMEFEEDEVTVGNNMMSSSPELGFEIDYLHRAPPQEVAPVGGWWGQMEFGQMGYRRDCEVPRMPVLSPAPAPMMWGENRSDSSKKGGVGNFNEMAAENSKLPPYPFSLLEGGF*