AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034647

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034647 MtrunA17_Chr6g0488471 95.082 244 6 2 1 244 52 289 5.68e-168 465
MsaG034647 MtrunA17_Chr8g0389241 50.000 226 76 8 26 244 85 280 1.16e-57 184
MsaG034647 MtrunA17_Chr2g0331031 70.000 90 20 1 29 111 85 174 5.02e-39 138
MsaG034647 MtrunA17_Chr4g0055931 65.217 92 28 1 24 111 69 160 2.86e-37 133
MsaG034647 MtrunA17_Chr1g0162081 60.784 102 33 2 40 134 116 217 1.02e-34 125
MsaG034647 MtrunA17_Chr4g0062741 73.239 71 19 0 40 110 132 202 2.47e-34 125
MsaG034647 MtrunA17_Chr7g0241761 78.873 71 15 0 40 110 134 204 7.14e-34 124
MsaG034647 MtrunA17_Chr5g0415191 65.909 88 27 1 26 110 79 166 1.37e-32 120
MsaG034647 MtrunA17_Chr3g0104071 73.611 72 19 0 40 111 141 212 5.75e-32 119
MsaG034647 MtrunA17_Chr5g0429521 74.648 71 18 0 40 110 126 196 7.72e-32 118
MsaG034647 MtrunA17_Chr1g0210471 71.429 70 20 0 41 110 119 188 9.95e-31 114
MsaG034647 MtrunA17_Chr4g0059701 76.712 73 17 0 38 110 76 148 1.51e-29 112
MsaG034647 MtrunA17_Chr5g0436501 69.863 73 20 1 40 110 119 191 1.75e-29 111
MsaG034647 MtrunA17_Chr3g0092621 60.976 82 30 1 31 110 67 148 2.40e-29 108
MsaG034647 MtrunA17_Chr3g0135671 60.714 84 29 2 43 123 88 170 3.26e-27 104
MsaG034647 MtrunA17_Chr6g0452791 67.797 59 19 0 46 104 80 138 5.47e-23 92.0
MsaG034647 MtrunA17_Chr5g0409841 52.857 70 31 1 42 109 88 157 1.53e-19 84.3
MsaG034647 MtrunA17_Chr5g0440731 50.704 71 32 1 42 109 101 171 3.15e-18 81.6
MsaG034647 MtrunA17_Chr5g0440741 55.385 65 26 1 48 109 102 166 5.31e-18 81.3
MsaG034647 MtrunA17_Chr5g0409831 58.929 56 23 0 42 97 73 128 4.39e-17 77.4
MsaG034647 MtrunA17_Chr8g0365011 51.948 77 23 2 48 110 94 170 1.37e-15 74.3
MsaG034647 MtrunA17_Chr1g0192061 50.633 79 33 3 48 123 63 138 1.97e-15 72.0
MsaG034647 MtrunA17_Chr8g0364731 64.912 57 18 1 56 110 11 67 2.85e-15 71.6
MsaG034647 MtrunA17_Chr5g0432921 47.500 80 38 3 48 124 115 193 9.37e-13 65.5
MsaG034647 MtrunA17_Chr5g0432941 46.250 80 39 3 48 124 115 193 2.85e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034647 AT5G56840.1 64.486 107 33 2 29 134 74 176 8.35e-43 145
MsaG034647 AT5G47390.1 70.652 92 24 1 23 111 70 161 5.69e-40 141
MsaG034647 AT5G61620.1 64.211 95 26 2 21 110 81 172 1.35e-35 129
MsaG034647 AT3G16350.1 43.049 223 89 8 10 206 85 295 1.13e-34 128
MsaG034647 AT1G49010.1 73.239 71 19 0 40 110 128 198 6.26e-34 124
MsaG034647 AT5G08520.1 67.949 78 25 0 40 117 112 189 2.11e-33 122
MsaG034647 AT2G38090.1 71.250 80 21 1 34 111 127 206 4.19e-33 122
MsaG034647 AT1G70000.1 67.442 86 25 1 29 111 78 163 6.10e-32 118
MsaG034647 AT1G70000.2 67.442 86 25 1 29 111 78 163 6.10e-32 118
MsaG034647 AT5G58900.1 74.286 70 18 0 41 110 136 205 2.45e-31 117
MsaG034647 AT5G01200.1 70.423 71 21 0 40 110 140 210 4.33e-30 113
MsaG034647 AT3G11280.2 69.014 71 22 0 40 110 121 191 6.55e-29 110
MsaG034647 AT3G11280.1 69.014 71 22 0 40 110 121 191 6.55e-29 110
MsaG034647 AT5G04760.1 65.714 70 24 0 41 110 94 163 1.30e-28 108
MsaG034647 AT5G05790.2 67.606 71 23 0 40 110 125 195 4.09e-28 108
MsaG034647 AT5G05790.1 67.606 71 23 0 40 110 125 195 4.09e-28 108
MsaG034647 AT1G19000.2 41.294 201 88 7 42 240 98 270 2.63e-27 106
MsaG034647 AT1G19000.1 41.294 201 88 7 42 240 98 270 2.63e-27 106
MsaG034647 AT1G74840.2 74.286 70 18 0 41 110 91 160 2.31e-26 102
MsaG034647 AT1G74840.1 74.286 70 18 0 41 110 91 160 3.22e-26 103
MsaG034647 AT5G23650.1 60.563 71 28 0 40 110 114 184 5.78e-24 98.6
MsaG034647 AT3G10580.2 56.627 83 34 1 42 124 91 171 1.68e-23 95.9
MsaG034647 AT3G10580.1 56.627 83 34 1 42 124 91 171 2.37e-23 95.9
MsaG034647 AT4G09450.1 57.971 69 29 0 42 110 87 155 2.33e-20 86.3
MsaG034647 AT3G10590.1 51.316 76 34 2 38 111 101 175 3.61e-16 75.1

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATTAAGTCACACTAAT+TGG 0.200852 6:-115617631 None:intergenic
TCCCCAAGTTCATTTCTTAT+TGG 0.248835 6:+115617607 MsaT034647.1:CDS
GCTGCTACATTAGAAGAATT+TGG 0.271044 6:-115617523 None:intergenic
CCCCAAGTTCATTTCTTATT+GGG 0.272509 6:+115617608 MsaT034647.1:CDS
TTGTTCCATGTTATTTGAAT+TGG 0.302002 6:-115617575 None:intergenic
CATATCATAATCATCTGATT+TGG 0.325159 6:-115617389 None:intergenic
ATATTTAAAGACTTTGTTAG+AGG 0.361817 6:-115617487 None:intergenic
ACGTCTCTTCTTCTTATTCA+AGG 0.365533 6:-115616363 None:intergenic
AGAAGCTTGGAAAGGGAGAT+TGG 0.367189 6:+115616255 MsaT034647.1:CDS
CTATGCTCTTCTTCTGTCCA+TGG 0.368105 6:-115616212 None:intergenic
GAGCATAGAAAATTCCTTGT+TGG 0.413219 6:+115616227 MsaT034647.1:CDS
CTTGTTGGACTTGAGAAGCT+TGG 0.417301 6:+115616242 MsaT034647.1:CDS
GTTAGAGGATGTAGCCAATC+TGG 0.418818 6:-115617472 None:intergenic
GCTTGGAAAGGGAGATTGGA+GGG 0.428206 6:+115616259 MsaT034647.1:CDS
ATTGATGATAGAGCCTCTAT+TGG 0.438397 6:+115615856 MsaT034647.1:CDS
AACATAAAGATGTGCCAGAT+TGG 0.460301 6:+115617458 MsaT034647.1:CDS
GAAGAAGAAAAGGAAGAAGT+AGG 0.461242 6:-115615823 None:intergenic
TCTATTGGTTATCTTTCAGA+TGG 0.468296 6:+115615871 MsaT034647.1:CDS
GGATAATCTTGAAGAAGAAA+AGG 0.479396 6:-115615833 None:intergenic
GATCTTGAGCTAACTCTGGC+AGG 0.479985 6:+115617550 MsaT034647.1:CDS
AATAGAGGCTCTATCATCAA+TGG 0.491413 6:-115615854 None:intergenic
GGACTTGAGAAGCTTGGAAA+GGG 0.498462 6:+115616248 MsaT034647.1:CDS
ATCTGAAAGATAACCAATAG+AGG 0.500436 6:-115615869 None:intergenic
AGCTTGGAAAGGGAGATTGG+AGG 0.502184 6:+115616258 MsaT034647.1:CDS
GGCCCAATAAGAAATGAACT+TGG 0.508375 6:-115617610 None:intergenic
TTTATTAATTAGGAGTTCCA+TGG 0.511389 6:+115616195 MsaT034647.1:intron
GCCAGAGTTAGCTCAAGATC+AGG 0.513405 6:-115617547 None:intergenic
TCATAGTTCGTACACAAGAA+AGG 0.523916 6:+115615896 MsaT034647.1:CDS
GCCCAATAAGAAATGAACTT+GGG 0.527445 6:-115617609 None:intergenic
GCATGGCTTGCAACTTGTGT+TGG 0.527834 6:-115616314 None:intergenic
GTAGGAGAAGAAGATGAAGT+AGG 0.532464 6:-115615805 None:intergenic
GAATATGTTTAGGTTGGTAG+TGG 0.550713 6:+115617232 MsaT034647.1:intron
AAAGTCTTTAAATATGACAT+TGG 0.555274 6:+115617495 MsaT034647.1:CDS
ACCTGATCTTGAGCTAACTC+TGG 0.566325 6:+115617546 MsaT034647.1:CDS
ATCTTGAGCTAACTCTGGCA+GGG 0.569532 6:+115617551 MsaT034647.1:CDS
TGGACTTGAGAAGCTTGGAA+AGG 0.582509 6:+115616247 MsaT034647.1:CDS
CCCAATAAGAAATGAACTTG+GGG 0.590590 6:-115617608 None:intergenic
CTTGGAAAGGGAGATTGGAG+GGG 0.594996 6:+115616260 MsaT034647.1:CDS
CAAGCTTCTCAAGTCCAACA+AGG 0.608243 6:-115616241 None:intergenic
TGATGATCATGAAGTTGAGA+AGG 0.617743 6:+115617324 MsaT034647.1:CDS
GATCATGAAGTTGAGAAGGA+TGG 0.622469 6:+115617328 MsaT034647.1:CDS
ACGTTCAAGTCTCTTTGACA+TGG 0.636224 6:+115616382 MsaT034647.1:CDS
AGGGTCCAATTCAAATAACA+TGG 0.651228 6:+115617570 MsaT034647.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 115615793 115617648 115615793 ID=MsaG034647
Chr6 mRNA 115615793 115617648 115615793 ID=MsaT034647.1;Parent=MsaG034647
Chr6 exon 115615793 115615925 115615793 ID=MsaT034647.1.exon1;Parent=MsaT034647.1
Chr6 CDS 115615793 115615925 115615793 ID=cds.MsaT034647.1;Parent=MsaT034647.1
Chr6 exon 115616207 115616403 115616207 ID=MsaT034647.1.exon2;Parent=MsaT034647.1
Chr6 CDS 115616207 115616403 115616207 ID=cds.MsaT034647.1;Parent=MsaT034647.1
Chr6 exon 115617244 115617648 115617244 ID=MsaT034647.1.exon3;Parent=MsaT034647.1
Chr6 CDS 115617244 115617648 115617244 ID=cds.MsaT034647.1;Parent=MsaT034647.1
Gene Sequence

>MsaT034647.1

ATGGATTCTTTTCCTACTTCATCTTCTTCTCCTACTTCTTCCTTTTCTTCTTCAAGATTATCCATTGATGATAGAGCCTCTATTGGTTATCTTTCAGATGGTCTCATAGTTCGTACACAAGAAAGGAAAAAAGGAGTTCCATGGACAGAAGAAGAGCATAGAAAATTCCTTGTTGGACTTGAGAAGCTTGGAAAGGGAGATTGGAGGGGTATCTCTAGAAACTATGTGACTACAAGAACACCAACACAAGTTGCAAGCCATGCTCAAAAGTATTTTATTCGACTTGCAACCTTGAATAAGAAGAAGAGACGTTCAAGTCTCTTTGACATGGTTGGTAGTGGCAAGACAAACAAAGCAGTTGATCCAAATAACAGCTCAAAAAGCAAGTCAGGTGATTCAGTTTGTAGACATGATGATCATGAAGTTGAGAAGGATGGAACTTTGTCACTACTAATAAACTCACTTCAACAACAAACCAAATCAGATGATTATGATATGCAAAAAATTGAAGATGTTGAAGTTGAAGATGATTCTGAAGAAGCAGAACATAAAGATGTGCCAGATTGGCTACATCCTCTAACAAAGTCTTTAAATATGACATTGGTGATACCAAATTCTTCTAATGTAGCAGCACCTGATCTTGAGCTAACTCTGGCAGGGTCCAATTCAAATAACATGGAACAAGATAAAACATCCCCAAGTTCATTTCTTATTGGGCCAATTAGTGTGACTTAA
Protein sequence

>MsaT034647.1

MDSFPTSSSSPTSSFSSSRLSIDDRASIGYLSDGLIVRTQERKKGVPWTEEEHRKFLVGLEKLGKGDWRGISRNYVTTRTPTQVASHAQKYFIRLATLNKKKRRSSLFDMVGSGKTNKAVDPNNSSKSKSGDSVCRHDDHEVEKDGTLSLLINSLQQQTKSDDYDMQKIEDVEVEDDSEEAEHKDVPDWLHPLTKSLNMTLVIPNSSNVAAPDLELTLAGSNSNNMEQDKTSPSSFLIGPISVT*