Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG034728 | RHN43614.1 | 55.769 | 156 | 59 | 2 | 1 | 146 | 1 | 156 | 1.17e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG034728 | tr|A0A396GVU6|A0A396GVU6_MEDTR | 55.769 | 156 | 59 | 2 | 1 | 146 | 1 | 156 | 5.57e-46 | 158 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG034728 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG034728 | MtrunA17_Chr8g0389811 | 55.769 | 156 | 59 | 2 | 1 | 146 | 1 | 156 | 1.07e-49 | 158 |
| MsaG034728 | MtrunA17_Chr1g0203051 | 55.446 | 101 | 45 | 0 | 39 | 139 | 44 | 144 | 6.18e-32 | 119 |
| MsaG034728 | MtrunA17_Chr8g0366311 | 50.909 | 110 | 49 | 1 | 1 | 105 | 1 | 110 | 4.18e-28 | 100 |
| MsaG034728 | MtrunA17_Chr6g0473421 | 47.826 | 92 | 48 | 0 | 55 | 146 | 36 | 127 | 2.67e-27 | 99.8 |
| MsaG034728 | MtrunA17_Chr1g0172931 | 42.975 | 121 | 60 | 3 | 35 | 146 | 28 | 148 | 5.52e-24 | 91.3 |
| MsaG034728 | MtrunA17_Chr2g0331371 | 44.660 | 103 | 52 | 1 | 55 | 152 | 36 | 138 | 7.27e-21 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGAGTATAGATGATTTA+TGG | 0.151163 | 6:+117143811 | MsaT034728.1:CDS |
| GCAACCTCTTCTGTTGTTCC+AGG | 0.291028 | 6:-117143708 | None:intergenic |
| AGCAGACCTTTGTATGTTAT+AGG | 0.299097 | 6:+117143952 | MsaT034728.1:CDS |
| TTGCTTATTATGTAGGATTT+GGG | 0.330556 | 6:+117143642 | MsaT034728.1:intron |
| CCAATTCAAGGGAATGCTTC+TGG | 0.366134 | 6:+117144145 | MsaT034728.1:intron |
| CCGCACCTCACACGAGCTCT+TGG | 0.404430 | 6:-117144026 | None:intergenic |
| GAGGTTGCAGATGGCACTAA+TGG | 0.478378 | 6:+117143723 | MsaT034728.1:CDS |
| GACAACTGCCGTGAATTGCT+TGG | 0.490891 | 6:+117143761 | MsaT034728.1:CDS |
| AATGTCTTCCAAGCAATTCA+CGG | 0.494836 | 6:-117143769 | None:intergenic |
| CCTTTGTATGTTATAGGCAA+GGG | 0.503564 | 6:+117143958 | MsaT034728.1:CDS |
| GCCGCAAAAGCAAAGTTGTA+AGG | 0.517061 | 6:+117143907 | MsaT034728.1:CDS |
| ACCTTTGTATGTTATAGGCA+AGG | 0.523646 | 6:+117143957 | MsaT034728.1:CDS |
| CACTAATGGTAACAAATTTG+AGG | 0.523859 | 6:+117143737 | MsaT034728.1:CDS |
| AATGAGCATAGTTATGATCC+TGG | 0.524091 | 6:+117143690 | MsaT034728.1:CDS |
| GGGAAATTTGTTATTGTCTG+AGG | 0.539782 | 6:+117143662 | MsaT034728.1:CDS |
| TCACCCAGTCGATTCACCAC+AGG | 0.578294 | 6:+117143548 | MsaT034728.1:CDS |
| CAAACCTGTGGTGAATCGAC+TGG | 0.589959 | 6:-117143552 | None:intergenic |
| AGGGAATGCTTCTGGCACAT+AGG | 0.590401 | 6:+117144153 | MsaT034728.1:intron |
| CCCTTGCCTATAACATACAA+AGG | 0.598990 | 6:-117143958 | None:intergenic |
| GGGATTAGTAAAGAGAAAAG+TGG | 0.608343 | 6:+117143978 | MsaT034728.1:CDS |
| ACAACAGAAGAGGTTGCAGA+TGG | 0.645232 | 6:+117143714 | MsaT034728.1:CDS |
| AAACCTGTGGTGAATCGACT+GGG | 0.650947 | 6:-117143551 | None:intergenic |
| TGATCCTGGAACAACAGAAG+AGG | 0.655464 | 6:+117143704 | MsaT034728.1:CDS |
| AAAGTTGTAAGGAACAACAA+AGG | 0.671963 | 6:+117143918 | MsaT034728.1:CDS |
| GTGTGCCAAGAGCTCGTGTG+AGG | 0.742198 | 6:+117144021 | MsaT034728.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 117143519 | 117144182 | 117143519 | ID=MsaG034728 |
| Chr6 | mRNA | 117143519 | 117144182 | 117143519 | ID=MsaT034728.1;Parent=MsaG034728 |
| Chr6 | exon | 117143519 | 117143569 | 117143519 | ID=MsaT034728.1.exon1;Parent=MsaT034728.1 |
| Chr6 | CDS | 117143519 | 117143569 | 117143519 | ID=cds.MsaT034728.1;Parent=MsaT034728.1 |
| Chr6 | exon | 117143657 | 117144042 | 117143657 | ID=MsaT034728.1.exon2;Parent=MsaT034728.1 |
| Chr6 | CDS | 117143657 | 117144042 | 117143657 | ID=cds.MsaT034728.1;Parent=MsaT034728.1 |
| Chr6 | exon | 117144155 | 117144182 | 117144155 | ID=MsaT034728.1.exon3;Parent=MsaT034728.1 |
| Chr6 | CDS | 117144155 | 117144182 | 117144155 | ID=cds.MsaT034728.1;Parent=MsaT034728.1 |
| Gene Sequence |
| Protein sequence |
