AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034778


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034778 MtrunA17_Chr7g0217231 95.107 327 10 3 17 342 20 341 0.0 518
MsaG034778 MtrunA17_Chr6g0453231 62.977 262 80 7 35 288 36 288 1.20e-76 238
MsaG034778 MtrunA17_Chr1g0188441 44.828 203 86 6 86 284 6 186 1.92e-52 171
MsaG034778 MtrunA17_Chr5g0404271 43.062 209 100 7 87 285 97 296 3.80e-48 164
MsaG034778 MtrunA17_Chr3g0133181 43.478 207 95 5 86 282 77 271 1.07e-47 162
MsaG034778 MtrunA17_Chr5g0412501 44.811 212 83 7 89 284 78 271 1.36e-46 159
MsaG034778 MtrunA17_Chr4g0076821 40.928 237 98 9 89 285 113 347 5.99e-45 157
MsaG034778 MtrunA17_Chr5g0427051 39.655 232 104 6 78 284 47 267 7.59e-45 154
MsaG034778 MtrunA17_Chr2g0315941 38.249 217 96 6 89 284 68 267 6.30e-40 141
MsaG034778 MtrunA17_Chr6g0479901 38.860 193 66 5 89 280 19 160 1.68e-37 132
MsaG034778 MtrunA17_Chr8g0367691 35.345 232 108 7 74 280 49 263 5.67e-36 131
MsaG034778 MtrunA17_Chr8g0361671 75.410 61 15 0 86 146 61 121 8.78e-27 109
MsaG034778 MtrunA17_Chr8g0357741 59.701 67 26 1 218 284 31 96 2.43e-19 81.6
MsaG034778 MtrunA17_Chr3g0092371 56.338 71 23 2 87 157 18 80 9.79e-18 77.0
MsaG034778 MtrunA17_Chr6g0453221 57.895 57 18 1 83 139 15 65 8.33e-16 71.6
MsaG034778 MtrunA17_Chr4g0060731 48.438 64 28 1 77 140 13 71 8.50e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034778 AT5G15210.1 49.817 273 100 9 22 286 3 246 1.57e-71 224
MsaG034778 AT1G69600.1 53.659 205 67 5 87 281 29 215 2.53e-65 207
MsaG034778 AT3G28920.1 48.693 306 105 10 22 300 1 281 1.67e-62 202
MsaG034778 AT5G39760.1 46.520 273 109 8 32 289 17 267 1.94e-55 184
MsaG034778 AT1G14440.1 43.363 226 105 5 84 300 85 296 1.08e-52 176
MsaG034778 AT1G14440.2 43.363 226 105 5 84 300 85 296 1.08e-52 176
MsaG034778 AT2G02540.2 42.152 223 108 6 86 296 84 297 6.21e-51 172
MsaG034778 AT2G02540.1 42.152 223 108 6 86 296 84 297 6.21e-51 172
MsaG034778 AT4G24660.1 45.455 198 83 4 87 284 47 219 4.27e-50 167
MsaG034778 AT4G24660.2 45.641 195 81 4 87 281 47 216 3.45e-49 165
MsaG034778 AT5G60480.1 43.939 198 78 5 86 278 34 203 1.88e-48 162
MsaG034778 AT5G65410.1 43.655 197 94 5 87 283 73 252 1.33e-44 154
MsaG034778 AT1G75240.1 38.559 236 104 4 84 283 71 301 1.76e-44 155
MsaG034778 AT2G18350.1 39.899 198 100 4 89 285 82 261 3.16e-41 145
MsaG034778 AT3G50890.1 39.252 214 99 8 89 295 60 249 1.48e-35 130
MsaG034778 AT5G42780.1 35.204 196 103 5 89 281 64 238 3.24e-28 110
MsaG034778 AT1G14687.1 28.283 198 106 4 84 279 2 165 4.34e-20 86.3
MsaG034778 AT1G74660.1 53.521 71 25 2 87 157 37 99 4.06e-19 81.6
MsaG034778 AT1G74660.2 61.111 54 19 1 87 140 37 88 8.18e-19 80.9
MsaG034778 AT1G18835.1 54.930 71 21 2 87 157 24 83 1.34e-17 77.0
MsaG034778 AT3G28917.1 61.404 57 21 1 86 142 30 85 2.97e-17 76.6

Find 96 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGTGTTGGTGTTGGAGTT+GGG 0.196157 7:-516114 None:intergenic
TGCTCTCTCCGCCGCCTTGC+CGG 0.214605 7:+516681 MsaT034778.1:CDS
ACAATTCTAGAAAGCGTTTC+AGG 0.216225 7:+516752 MsaT034778.1:CDS
GTGGAAAGTGGTGGCTCTTC+AGG 0.239612 7:-516517 None:intergenic
TTGAGAACACCATTTGAAAA+AGG 0.255600 7:-516265 None:intergenic
GGTGGTGTTGGTGTTGGAGT+TGG 0.272963 7:-516115 None:intergenic
AGGGGCGGAGGGGTAGTAAG+AGG 0.274253 7:-516647 None:intergenic
GGTGGTGGTGAGGAGGAATT+TGG 0.278567 7:-516616 None:intergenic
TCAGTTTCATGTTGTTGTTC+TGG 0.293454 7:-516196 None:intergenic
TAATATTCTCATTGTTGTTA+AGG 0.328995 7:-516954 None:intergenic
TGGTGTTGGAGTTGGGGTTA+AGG 0.330499 7:-516107 None:intergenic
TGTTGTTGAGGTTGTTAATA+TGG 0.330756 7:-516158 None:intergenic
ATCGTAGTGTTCTTAAGGTT+TGG 0.334173 7:+516893 MsaT034778.1:CDS
GAGACATTCTTTGTAGGTTA+TGG 0.340619 7:-516353 None:intergenic
GGTTGAAGTGGTGGTGGTGG+TGG 0.346158 7:-516300 None:intergenic
TTCATCAACATGGTTTGGTT+TGG 0.350778 7:-516718 None:intergenic
TGTTGATCGTAGTGTTCTTA+AGG 0.355414 7:+516888 MsaT034778.1:CDS
CAGAATGAAGGTGGAGTTGT+TGG 0.373866 7:+517063 MsaT034778.1:CDS
GGAGTAACTGTTCGTGCTAA+TGG 0.375764 7:+517084 MsaT034778.1:CDS
AAGAAGCATGTGAGGGGCGG+AGG 0.375917 7:-516659 None:intergenic
GAGAATTCATCAACATGGTT+TGG 0.380377 7:-516723 None:intergenic
TGTAGGTTATGGTAGGTTGA+TGG 0.383764 7:-516342 None:intergenic
CACAAGAATGTTGAGATTCA+TGG 0.386670 7:+517021 MsaT034778.1:CDS
ATTTGGGCTCCGGTGGGAAA+AGG 0.392682 7:-516599 None:intergenic
GTTGGTGGTGGTGTTGGTGT+TGG 0.400036 7:-516121 None:intergenic
TGGTGTTGGTGTTGGAGTTG+GGG 0.405042 7:-516113 None:intergenic
CTGAAATTTGCTGAGAAAGT+TGG 0.415196 7:+516808 MsaT034778.1:CDS
GCTCTTCAGGTTCACGGCGG+TGG 0.421733 7:-516504 None:intergenic
TGAATTCACCACAACCATCT+AGG 0.423468 7:-516408 None:intergenic
TATGGTAGGTTGATGGTTGT+TGG 0.424329 7:-516335 None:intergenic
ATTGATGTTGGTGGTGGTGT+TGG 0.426088 7:-516127 None:intergenic
CGGTGGGAAAAGGGTGGTGG+TGG 0.426898 7:-516589 None:intergenic
GAAAAGGGTGGTGGTGGAAG+TGG 0.427112 7:-516583 None:intergenic
TTGTTCTGGTGATTTAGTTA+AGG 0.427703 7:-516182 None:intergenic
CTCCGGTGGGAAAAGGGTGG+TGG 0.429757 7:-516592 None:intergenic
GGTGGTTGAAGTGGTGGTGG+TGG 0.435301 7:-516303 None:intergenic
GGAGGAGATCGGTGGTGGTG+AGG 0.437213 7:-516626 None:intergenic
GGGTAGTAAGAGGAGGAGAT+CGG 0.438670 7:-516637 None:intergenic
GTTGAGGTTGTTAATATGGT+TGG 0.439657 7:-516154 None:intergenic
GGCGGAGGGGTAGTAAGAGG+AGG 0.440166 7:-516644 None:intergenic
GTGGTGGTGAGGAGGAATTT+GGG 0.444504 7:-516615 None:intergenic
CTAGGTGGTCATGCCCTAGA+TGG 0.446320 7:+516394 MsaT034778.1:CDS
GGGCTCCGGTGGGAAAAGGG+TGG 0.457386 7:-516595 None:intergenic
GTTCACGGCGGTGGAAGTTA+CGG 0.458240 7:-516495 None:intergenic
TTTAAGGATGTTGGGTCGGT+TGG 0.464594 7:-516451 None:intergenic
TGAAATTTGCTGAGAAAGTT+GGG 0.467964 7:+516809 MsaT034778.1:CDS
TTCAATGACATGGGTGGTTG+TGG 0.469669 7:-516536 None:intergenic
GGGTCGGTTGGTGTTGCTGT+GGG 0.476226 7:-516439 None:intergenic
TGTGGTGGTTGAAGTGGTGG+TGG 0.478966 7:-516306 None:intergenic
ACATGGGTGGTTGTGGAAAG+TGG 0.487507 7:-516529 None:intergenic
ATGGTTGGTGCTATTGATGT+TGG 0.487966 7:-516139 None:intergenic
GGAGATCGGTGGTGGTGAGG+AGG 0.490231 7:-516623 None:intergenic
CACAATCATTATCAGAATGA+AGG 0.494541 7:+517051 MsaT034778.1:CDS
AGAAGCATGTGAGGGGCGGA+GGG 0.495592 7:-516658 None:intergenic
GGTTGTTGGTGTTGTTGTGG+TGG 0.497322 7:-516321 None:intergenic
TAAGAGGAGGAGATCGGTGG+TGG 0.498462 7:-516631 None:intergenic
TGGGGTTAAGGTTATGTCCA+TGG 0.499789 7:-516095 None:intergenic
GTGAGGAGGAATTTGGGCTC+CGG 0.500275 7:-516609 None:intergenic
GTGGTGGAAGTGGTTGATGA+CGG 0.500583 7:-516573 None:intergenic
TTTAGTTAAGGTTGTTGTTG+AGG 0.502769 7:-516170 None:intergenic
AGGAGGAATTTGGGCTCCGG+TGG 0.505710 7:-516606 None:intergenic
TTTGGGCTCCGGTGGGAAAA+GGG 0.507171 7:-516598 None:intergenic
GATGGTTGTTGGTGTTGTTG+TGG 0.510023 7:-516324 None:intergenic
CATTCTTTGTAGGTTATGGT+AGG 0.517382 7:-516349 None:intergenic
GGTGCTATTGATGTTGGTGG+TGG 0.518237 7:-516133 None:intergenic
GTGGTGGCTCTTCAGGTTCA+CGG 0.521077 7:-516510 None:intergenic
GTTGGTGCTATTGATGTTGG+TGG 0.524202 7:-516136 None:intergenic
AACAATGAGAATATTAATGA+TGG 0.527160 7:+516961 MsaT034778.1:CDS
TGTTGTTGTGGTGGTTGAAG+TGG 0.528276 7:-516312 None:intergenic
GACCACCTAGGTTTGCTGCA+TGG 0.528612 7:-516381 None:intergenic
TGTTGTGGTGGTTGAAGTGG+TGG 0.528860 7:-516309 None:intergenic
GAATTCACCACAACCATCTA+GGG 0.539304 7:-516407 None:intergenic
GAGAGAGCAAGAAGCATGTG+AGG 0.550052 7:-516667 None:intergenic
GAAGCATGTGAGGGGCGGAG+GGG 0.553313 7:-516657 None:intergenic
TGGGTCGGTTGGTGTTGCTG+TGG 0.558596 7:-516440 None:intergenic
TCCTTAAAATGTGCTGCTTG+TGG 0.574232 7:+516466 MsaT034778.1:CDS
GGTCATGCCCTAGATGGTTG+TGG 0.575703 7:+516400 MsaT034778.1:CDS
TAGTAAGAGGAGGAGATCGG+TGG 0.577001 7:-516634 None:intergenic
TTGTGTTCTTCATCTTCAAG+AGG 0.585430 7:-517003 None:intergenic
AGCAAGAAGCATGTGAGGGG+CGG 0.594492 7:-516662 None:intergenic
AGAGAGCAAGAAGCATGTGA+GGG 0.597359 7:-516666 None:intergenic
AATCATTATCAGAATGAAGG+TGG 0.598316 7:+517054 MsaT034778.1:CDS
GTGGAAGTGGTTGATGACGG+TGG 0.599219 7:-516570 None:intergenic
GAGAGCAAGAAGCATGTGAG+GGG 0.602424 7:-516665 None:intergenic
AGTGGTTGATGACGGTGGTG+AGG 0.625162 7:-516565 None:intergenic
GGAGGAATTTGGGCTCCGGT+GGG 0.630361 7:-516605 None:intergenic
TGGGTGGTTGTGGAAAGTGG+TGG 0.636395 7:-516526 None:intergenic
AGAGTGAGAATTCATCAACA+TGG 0.636406 7:-516728 None:intergenic
CATCTAGGGCATGACCACCT+AGG 0.648371 7:-516393 None:intergenic
CACGGCGGTGGAAGTTACGG+TGG 0.670619 7:-516492 None:intergenic
AACCATGCAGCAAACCTAGG+TGG 0.685477 7:+516379 MsaT034778.1:CDS
GAGGTTGATATTCAATGACA+TGG 0.686425 7:-516546 None:intergenic
AATTTGCTGAGAAAGTTGGG+TGG 0.726025 7:+516812 MsaT034778.1:CDS
AGGTTGATATTCAATGACAT+GGG 0.727348 7:-516545 None:intergenic
TTGATATTCAATGACATGGG+TGG 0.731584 7:-516542 None:intergenic
GTGGCTCTTCAGGTTCACGG+CGG 0.764896 7:-516507 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 516097 517125 516097 ID=MsaG034778
Chr7 mRNA 516097 517125 516097 ID=MsaT034778.1;Parent=MsaG034778
Chr7 exon 516097 517125 516097 ID=MsaT034778.1.exon1;Parent=MsaT034778.1
Chr7 CDS 516097 517125 516097 ID=cds.MsaT034778.1;Parent=MsaT034778.1
Gene Sequence

>MsaT034778.1

ATGGACATAACCTTAACCCCAACTCCAACACCAACACCACCACCAACATCAATAGCACCAACCATATTAACAACCTCAACAACAACCTTAACTAAATCACCAGAACAACAACATGAAACTGAAACACCCACAACAAAATTCCCAACCTCAACAACACCAAAAATCTCACCTTTTTCAAATGGTGTTCTCAAACGTTTCCACCACCACCACCACCACCACTTCAACCACCACAACAACACCAACAACCATCAACCTACCATAACCTACAAAGAATGTCTCAAAAACCATGCAGCAAACCTAGGTGGTCATGCCCTAGATGGTTGTGGTGAATTCATGACATCACCCACAGCAACACCAACCGACCCAACATCCTTAAAATGTGCTGCTTGTGGCTGCCACCGTAACTTCCACCGCCGTGAACCTGAAGAGCCACCACTTTCCACAACCACCCATGTCATTGAATATCAACCTCACCACCGTCATCAACCACTTCCACCACCACCCTTTTCCCACCGGAGCCCAAATTCCTCCTCACCACCACCGATCTCCTCCTCTTACTACCCCTCCGCCCCTCACATGCTTCTTGCTCTCTCCGCCGCCTTGCCGGAAAACGTTGCAGCTCCAAACCAAACCATGTTGATGAATTCTCACTCTAACAATTCTAGAAAGCGTTTCAGGACAAAGTTTACACAAGATCAGAAAGATAAGATGCTGAAATTTGCTGAGAAAGTTGGGTGGAAGATGCAGAAAAAAGATGATGAATTTGTTCATGAGTTTTGTAATGAGATTGGTGTTGATCGTAGTGTTCTTAAGGTTTGGATGCATAACAACAAGAACACTTTAGCCAAACGAGAAAACCTTAACAACAATGAGAATATTAATGATGGTGTTAAGAGTTTTCAGCTACCTCTTGAAGATGAAGAACACAAGAATGTTGAGATTCATGGTCTTAATCACAATCATTATCAGAATGAAGGTGGAGTTGTTGGAGTAACTGTTCGTGCTAATGGTTCATCATCTTCTTCTTAA

Protein sequence

>MsaT034778.1

MDITLTPTPTPTPPPTSIAPTILTTSTTTLTKSPEQQHETETPTTKFPTSTTPKISPFSNGVLKRFHHHHHHHFNHHNNTNNHQPTITYKECLKNHAANLGGHALDGCGEFMTSPTATPTDPTSLKCAACGCHRNFHRREPEEPPLSTTTHVIEYQPHHRHQPLPPPPFSHRSPNSSSPPPISSSYYPSAPHMLLALSAALPENVAAPNQTMLMNSHSNNSRKRFRTKFTQDQKDKMLKFAEKVGWKMQKKDDEFVHEFCNEIGVDRSVLKVWMHNNKNTLAKRENLNNNENINDGVKSFQLPLEDEEHKNVEIHGLNHNHYQNEGGVVGVTVRANGSSSSS*