AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034784

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034784 MtrunA17_Chr7g0217111 96.691 272 9 0 1 272 1 272 0.0 546
MsaG034784 MtrunA17_Chr6g0453021 60.825 291 73 10 1 272 1 269 2.02e-108 315
MsaG034784 MtrunA17_Chr8g0362151 51.761 284 115 9 1 271 1 275 6.24e-77 235
MsaG034784 MtrunA17_Chr5g0416391 49.291 282 123 8 1 271 4 276 1.36e-69 216
MsaG034784 MtrunA17_Chr5g0415941 59.459 111 43 1 65 175 74 182 4.95e-33 123
MsaG034784 MtrunA17_Chr3g0119291 50.360 139 57 3 71 197 47 185 6.19e-33 120
MsaG034784 MtrunA17_Chr4g0060031 40.654 214 108 6 48 252 57 260 1.04e-31 119
MsaG034784 MtrunA17_Chr6g0450991 58.416 101 41 1 75 175 63 162 3.30e-30 114
MsaG034784 MtrunA17_Chr3g0123691 59.794 97 39 0 79 175 59 155 3.65e-30 114
MsaG034784 MtrunA17_Chr8g0381741 57.732 97 41 0 79 175 53 149 4.14e-29 112
MsaG034784 MtrunA17_Chr7g0265521 47.692 130 64 2 67 196 51 176 1.40e-28 108
MsaG034784 MtrunA17_Chr8g0362751 58.333 96 40 0 80 175 89 184 7.56e-27 105
MsaG034784 MtrunA17_Chr1g0179581 58.537 82 34 0 78 159 26 107 7.65e-25 95.5
MsaG034784 MtrunA17_Chr4g0055851 39.241 158 87 2 16 173 61 209 3.16e-24 98.6
MsaG034784 MtrunA17_Chr3g0127511 44.355 124 60 3 83 200 41 161 9.50e-22 91.3
MsaG034784 MtrunA17_Chr8g0346251 49.451 91 39 1 87 170 48 138 6.11e-21 89.0
MsaG034784 MtrunA17_Chr5g0399491 42.537 134 63 3 49 173 97 225 2.03e-20 88.6
MsaG034784 MtrunA17_Chr7g0258831 40.157 127 69 1 48 174 128 247 4.10e-20 87.8
MsaG034784 MtrunA17_Chr1g0175651 45.714 105 50 1 70 174 144 241 1.02e-19 86.7
MsaG034784 MtrunA17_Chr1g0151971 48.958 96 42 1 79 174 129 217 2.92e-19 85.1
MsaG034784 MtrunA17_Chr4g0054421 44.860 107 51 2 68 174 116 214 4.66e-19 84.3
MsaG034784 MtrunA17_Chr5g0400781 45.192 104 50 1 71 174 114 210 5.29e-19 84.3
MsaG034784 MtrunA17_Chr8g0335131 49.474 95 44 1 79 173 145 235 1.26e-18 83.6
MsaG034784 MtrunA17_Chr2g0308611 44.954 109 56 2 68 176 188 292 2.22e-18 83.6
MsaG034784 MtrunA17_Chr4g0071411 48.235 85 44 0 82 166 90 174 5.00e-18 80.5
MsaG034784 MtrunA17_Chr4g0044091 45.263 95 48 1 79 173 64 154 1.64e-16 75.9
MsaG034784 MtrunA17_Chr5g0403701 48.889 90 46 0 80 169 53 142 9.79e-16 73.9
MsaG034784 MtrunA17_Chr5g0403691 54.167 72 33 0 80 151 53 124 8.70e-15 71.6
MsaG034784 MtrunA17_Chr3g0135331 32.298 161 93 3 59 205 120 278 5.82e-14 70.5
MsaG034784 MtrunA17_Chr2g0299031 43.299 97 51 1 77 173 79 171 1.18e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034784 AT3G01220.1 44.444 279 128 12 1 272 16 274 6.29e-64 202
MsaG034784 AT5G15150.1 45.051 293 125 12 1 271 22 300 1.28e-61 197
MsaG034784 AT1G69780.1 43.493 292 133 11 1 271 7 287 1.13e-59 191
MsaG034784 AT1G26960.1 42.238 277 118 11 1 271 8 248 9.84e-53 172
MsaG034784 AT5G65310.1 45.509 167 81 3 22 180 8 172 1.77e-33 124
MsaG034784 AT5G65310.2 50.000 136 63 1 50 180 19 154 1.94e-33 123
MsaG034784 AT2G22430.1 57.732 97 41 0 79 175 61 157 1.15e-30 116
MsaG034784 AT4G40060.1 56.701 97 42 0 79 175 58 154 1.87e-30 115
MsaG034784 AT3G01470.1 56.701 97 42 0 79 175 67 163 1.11e-29 113
MsaG034784 AT2G46680.1 50.549 91 45 0 83 173 35 125 7.19e-24 97.4
MsaG034784 AT1G27045.1 40.157 127 76 0 49 175 37 163 7.16e-23 94.0
MsaG034784 AT1G27045.4 40.323 124 74 0 49 172 37 160 3.10e-22 93.2
MsaG034784 AT5G03790.1 47.664 107 54 1 80 184 77 183 3.74e-22 92.4
MsaG034784 AT1G27045.5 44.545 110 61 0 66 175 11 120 5.98e-22 90.5
MsaG034784 AT1G27045.3 44.545 110 61 0 66 175 11 120 5.98e-22 90.5
MsaG034784 AT1G27045.2 44.545 110 61 0 66 175 3 112 7.28e-22 90.1
MsaG034784 AT3G61890.1 48.889 90 46 0 83 172 33 122 9.36e-22 91.3
MsaG034784 AT2G46680.2 48.837 86 44 0 83 168 35 120 2.14e-21 90.9
MsaG034784 AT5G03790.2 49.485 97 47 1 80 176 77 171 5.08e-21 88.2
MsaG034784 AT2G36610.1 45.946 111 45 2 72 174 63 166 4.70e-20 85.5
MsaG034784 AT4G17460.1 43.478 115 61 1 59 173 114 224 6.40e-20 87.4
MsaG034784 AT2G18550.1 33.871 186 96 5 15 195 7 170 2.77e-19 84.3
MsaG034784 AT5G47370.1 39.706 136 69 4 45 173 90 219 3.60e-19 85.1
MsaG034784 AT5G53980.1 47.872 94 49 0 77 170 8 101 9.58e-19 81.3
MsaG034784 AT3G60390.1 45.536 112 52 3 65 173 146 251 9.72e-19 84.7
MsaG034784 AT4G16780.1 44.545 110 57 1 64 173 113 218 1.30e-18 83.6
MsaG034784 AT5G06710.1 47.917 96 46 1 79 174 189 280 3.63e-18 83.2
MsaG034784 AT2G01430.1 44.118 102 57 0 66 167 125 226 4.90e-18 82.0
MsaG034784 AT4G37790.1 45.192 104 50 1 71 174 117 213 6.76e-18 81.6
MsaG034784 AT2G22800.1 42.241 116 51 3 59 174 101 200 3.14e-17 79.7
MsaG034784 AT2G44910.1 46.316 95 47 1 79 173 162 252 9.62e-17 79.0
MsaG034784 AT4G36740.1 42.056 107 57 1 80 181 55 161 3.35e-16 75.9
MsaG034784 AT4G36740.2 42.056 107 57 1 80 181 56 162 3.66e-16 75.9
MsaG034784 AT1G70920.1 41.818 110 58 2 64 173 55 158 1.67e-15 73.6
MsaG034784 AT1G70920.2 41.818 110 58 2 64 173 25 128 1.91e-15 72.8
MsaG034784 AT5G66700.1 48.750 80 38 1 66 145 60 136 1.42e-14 71.6
MsaG034784 AT5G06710.4 54.717 53 24 0 79 131 189 241 1.46e-11 63.5
MsaG034784 AT2G01430.2 48.485 66 34 0 66 131 125 190 2.32e-11 62.4

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAGAAGAGCTTTGAATT+TGG 0.186029 7:+749071 MsaT034784.1:CDS
GTGCAACTTGCTAAGGCTTT+AGG 0.214413 7:+749119 MsaT034784.1:CDS
GAAGAAGATCAGGCTGAATT+TGG 0.249178 7:+749034 MsaT034784.1:CDS
TGCAACTTGCTAAGGCTTTA+GGG 0.260540 7:+749120 MsaT034784.1:CDS
AAGTTGGTAGAAGGCTAATT+AGG 0.295400 7:-750997 None:intergenic
AGAAGTTGGGGTATATTGTT+AGG 0.301981 7:-751028 None:intergenic
TTGAGAAGAGCTTTGAATTT+GGG 0.313267 7:+749072 MsaT034784.1:CDS
AGCCTAGACAAATTGCTATA+TGG 0.333381 7:+749147 MsaT034784.1:CDS
TGAAAGAAACTGAGGGTTCA+TGG 0.388104 7:+750882 MsaT034784.1:CDS
AGCATCAGAACCTCTGGCCT+TGG 0.392592 7:+751116 MsaT034784.1:CDS
GGTTTAATGAAGTTGGTAGA+AGG 0.399111 7:-751006 None:intergenic
TGATGTGAATTGAAGAAGTT+GGG 0.408685 7:-751041 None:intergenic
GCTGAATTTGGATCAAGTTC+AGG 0.410690 7:+749046 MsaT034784.1:CDS
GTGGAGAGAAGAAGAAGATC+AGG 0.413666 7:+749024 MsaT034784.1:CDS
TTGTTAGGGTTTAATGAAGT+TGG 0.422091 7:-751013 None:intergenic
TCTTCTTCTTCTCTCCACTT+TGG 0.431006 7:-749019 None:intergenic
TTGATGTGAATTGAAGAAGT+TGG 0.467181 7:-751042 None:intergenic
GATGATGATTTATCTGATGA+TGG 0.471832 7:+748993 MsaT034784.1:CDS
AAGTGTTGAGGTGGAAAAGA+AGG 0.489869 7:-748449 None:intergenic
AACAAGAAACTATGAGGTGG+TGG 0.493336 7:-748374 None:intergenic
GAAGTTGGGGTATATTGTTA+GGG 0.501507 7:-751027 None:intergenic
AAGAAAAGTGCAACTTGCTA+AGG 0.504058 7:+749112 MsaT034784.1:CDS
TTGATGAGCATCAGAACCTC+TGG 0.507447 7:+751110 MsaT034784.1:CDS
AGTGTTGAGGTGGAAAAGAA+GGG 0.508674 7:-748448 None:intergenic
CAAATTCAAAGCTCTTCTCA+AGG 0.519428 7:-749070 None:intergenic
AGTTCTGTTTCTTCACAGAA+TGG 0.523751 7:+750968 MsaT034784.1:CDS
GAAGAAATTTGAAGCTGTCA+AGG 0.526329 7:+749232 MsaT034784.1:CDS
AAAAGCAAGGATTGGTGTGA+AGG 0.539700 7:+750842 MsaT034784.1:CDS
CCACCTCAACACTTTCAACA+AGG 0.552469 7:+748458 MsaT034784.1:CDS
CCTTGTTGAAAGTGTTGAGG+TGG 0.554667 7:-748458 None:intergenic
AAGGTGTTAAGAGAAGTTGA+AGG 0.567749 7:-748425 None:intergenic
TTGAGGTGGAAAAGAAGGGA+AGG 0.568416 7:-748444 None:intergenic
AGCAAGGATTGGTGTGAAGG+TGG 0.575668 7:+750845 MsaT034784.1:CDS
AGGTGTTAAGAGAAGTTGAA+GGG 0.587354 7:-748424 None:intergenic
AAATTCAAAGCTCTTCTCAA+GGG 0.589863 7:-749069 None:intergenic
ATCAATGTTGTGAAACATGT+TGG 0.592933 7:-751092 None:intergenic
AACCATATAGCAATTTGTCT+AGG 0.597887 7:-749149 None:intergenic
ATGCATAATGAAAGAAACTG+AGG 0.602715 7:+750874 MsaT034784.1:CDS
ACTGAGGGTTCATGGAGCAA+TGG 0.602832 7:+750890 MsaT034784.1:CDS
ACAAGAAACTATGAGGTGGT+GGG 0.605048 7:-748373 None:intergenic
TCTGATGATGGATTCCAAAG+TGG 0.607438 7:+749005 MsaT034784.1:CDS
TAATCAGAAACTACAAGGAG+AGG 0.617126 7:+749280 MsaT034784.1:CDS
GTTCATAATCAGAAACTACA+AGG 0.617357 7:+749275 MsaT034784.1:CDS
AGTTGATCATGATCTTCATG+AGG 0.620183 7:-748401 None:intergenic
AAATGATGCATCTATGTCCA+AGG 0.620204 7:-751133 None:intergenic
GGGTTAAACAAGAAACTATG+AGG 0.624170 7:-748380 None:intergenic
CATGATCATCATGAAGTGCA+TGG 0.624861 7:+748969 MsaT034784.1:CDS
TGACCTTGTTGAAAGTGTTG+AGG 0.631537 7:-748461 None:intergenic
TGCATAATGAAAGAAACTGA+GGG 0.634219 7:+750875 MsaT034784.1:CDS
GTTGATCATGATCTTCATGA+GGG 0.635617 7:-748400 None:intergenic
GCATCTATGTCCAAGGCCAG+AGG 0.638650 7:-751126 None:intergenic
GATGTGAATTGAAGAAGTTG+GGG 0.704596 7:-751040 None:intergenic
TTAAACAAGAAACTATGAGG+TGG 0.721896 7:-748377 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 748365 751144 748365 ID=MsaG034784
Chr7 mRNA 748365 751144 748365 ID=MsaT034784.1;Parent=MsaG034784
Chr7 exon 748365 748479 748365 ID=MsaT034784.1.exon1;Parent=MsaT034784.1
Chr7 CDS 748365 748479 748365 ID=cds.MsaT034784.1;Parent=MsaT034784.1
Chr7 exon 748913 749301 748913 ID=MsaT034784.1.exon2;Parent=MsaT034784.1
Chr7 CDS 748913 749301 748913 ID=cds.MsaT034784.1;Parent=MsaT034784.1
Chr7 exon 750830 751144 750830 ID=MsaT034784.1.exon3;Parent=MsaT034784.1
Chr7 CDS 750830 751144 750830 ID=cds.MsaT034784.1;Parent=MsaT034784.1
Gene Sequence

>MsaT034784.1

ATGGCATTCCCACCACCTCATAGTTTCTTGTTTAACCCTCATGAAGATCATGATCAACTCCCTTCAACTTCTCTTAACACCTTCCCTTCTTTTCCACCTCAACACTTTCAACAAGGTGCACCTTTTTTGTTGAAGAAATCAATGTCATTTTCGGGCATTGAGAACAAATGTCATGATCATCATGAAGTGCATGGAGATGATGATTTATCTGATGATGGATTCCAAAGTGGAGAGAAGAAGAAGATCAGGCTGAATTTGGATCAAGTTCAGGCCCTTGAGAAGAGCTTTGAATTTGGGAACAAGCTTGACCCAGAAAGAAAAGTGCAACTTGCTAAGGCTTTAGGGTTGCAGCCTAGACAAATTGCTATATGGTTTCAGAATAGAAGAGCAAGATGCAAAACAAAGCAACTTGAGAATGAATATGAAGTACTCAAGAAGAAATTTGAAGCTGTCAAGGATGAAAATGATGCACTTAAAGTTCATAATCAGAAACTACAAGGAGAGTTACAAGCATTAAAAAGCAAGGATTGGTGTGAAGGTGGAACAAAATGCATAATGAAAGAAACTGAGGGTTCATGGAGCAATGGAAGTGATAATAGCTTAAACATTAATTTAGATCTCTCAAGAACACAAGCTTTGAACAGTTCTGTTTCTTCACAGAATGGTAGAAACCTAATTAGCCTTCTACCAACTTCATTAAACCCTAACAATATACCCCAACTTCTTCAATTCACATCAAGACCAGATCTCCAAGATGAAAACTTCACCAACATGTTTCACAACATTGATGAGCATCAGAACCTCTGGCCTTGGACATAG
Protein sequence

>MsaT034784.1

MAFPPPHSFLFNPHEDHDQLPSTSLNTFPSFPPQHFQQGAPFLLKKSMSFSGIENKCHDHHEVHGDDDLSDDGFQSGEKKKIRLNLDQVQALEKSFEFGNKLDPERKVQLAKALGLQPRQIAIWFQNRRARCKTKQLENEYEVLKKKFEAVKDENDALKVHNQKLQGELQALKSKDWCEGGTKCIMKETEGSWSNGSDNSLNINLDLSRTQALNSSVSSQNGRNLISLLPTSLNPNNIPQLLQFTSRPDLQDENFTNMFHNIDEHQNLWPWT*