AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG034850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034850 MtrunA17_Chr7g0223971 83.398 518 53 1 59 543 1 518 0.0 815
MsaG034850 MtrunA17_Chr7g0223991 76.056 213 31 3 214 424 24 218 2.02e-99 305
MsaG034850 MtrunA17_Chr6g0473731 45.370 432 166 12 57 471 10 388 1.29e-96 306
MsaG034850 MtrunA17_Chr2g0294911 39.810 211 115 4 5 203 57 267 1.37e-39 154
MsaG034850 MtrunA17_Chr4g0067981 36.150 213 122 2 5 203 59 271 1.02e-38 152
MsaG034850 MtrunA17_Chr1g0161431 33.740 246 138 5 4 225 38 282 1.16e-38 152
MsaG034850 MtrunA17_Chr1g0149841 36.000 225 114 3 4 205 32 249 1.06e-37 147
MsaG034850 MtrunA17_Chr8g0376881 34.894 235 135 5 5 221 53 287 1.47e-37 147
MsaG034850 MtrunA17_Chr7g0215541 57.746 142 49 2 549 687 92 225 1.75e-37 143
MsaG034850 MtrunA17_Chr3g0123131 33.460 263 147 6 5 242 41 300 3.52e-37 147
MsaG034850 MtrunA17_Chr3g0137101 37.500 224 116 4 4 205 35 256 2.41e-36 145
MsaG034850 MtrunA17_Chr7g0275401 36.818 220 120 3 2 203 30 248 6.42e-36 137
MsaG034850 MtrunA17_Chr4g0009851 31.419 296 172 8 5 291 39 312 6.54e-35 135
MsaG034850 MtrunA17_Chr4g0009981 29.391 279 167 6 22 291 2 259 7.04e-35 133
MsaG034850 MtrunA17_Chr4g0009901 35.784 204 111 3 4 203 37 224 3.44e-34 132
MsaG034850 MtrunA17_Chr6g0471041 35.238 210 122 3 1 209 38 234 5.92e-34 134
MsaG034850 MtrunA17_Chr7g0275891 32.618 233 124 5 4 205 32 262 1.69e-33 132
MsaG034850 MtrunA17_Chr2g0304841 32.917 240 130 4 4 214 45 282 9.68e-33 134
MsaG034850 MtrunA17_Chr3g0134481 33.333 195 84 2 4 198 35 183 1.45e-31 121
MsaG034850 MtrunA17_Chr4g0074411 32.589 224 138 2 1 211 42 265 1.95e-31 130
MsaG034850 MtrunA17_Chr3g0134461 34.536 194 90 2 4 196 35 192 3.52e-30 117
MsaG034850 MtrunA17_Chr1g0149851 33.193 238 114 4 4 203 32 262 1.23e-29 124
MsaG034850 MtrunA17_Chr4g0074401 31.441 229 141 3 1 213 41 269 3.05e-29 123
MsaG034850 MtrunA17_Chr4g0074421 35.638 188 108 3 26 201 1 187 1.28e-26 107
MsaG034850 MtrunA17_Chr1g0149831 34.359 195 87 5 34 205 7 183 2.79e-24 102
MsaG034850 MtrunA17_Chr2g0277281 41.667 156 81 4 545 697 138 286 2.10e-21 97.4
MsaG034850 MtrunA17_Chr7g0227241 45.455 132 69 3 569 699 24 153 8.71e-21 93.2
MsaG034850 MtrunA17_Chr2g0278091 44.167 120 58 3 587 697 135 254 7.37e-20 92.4
MsaG034850 MtrunA17_Chr4g0009991 37.121 132 61 3 70 196 2 116 7.54e-20 85.9
MsaG034850 MtrunA17_Chr3g0132631 46.903 113 60 0 585 697 147 259 1.53e-19 91.3
MsaG034850 MtrunA17_Chr2g0278791 42.177 147 71 4 549 687 117 257 8.73e-19 89.4
MsaG034850 MtrunA17_Chr4g0031961 42.857 112 48 4 124 225 101 206 1.39e-18 87.4
MsaG034850 MtrunA17_Chr7g0255711 49.296 71 36 0 136 206 80 150 4.73e-18 85.5
MsaG034850 MtrunA17_Chr4g0011071 28.477 151 89 3 27 173 39 174 1.98e-17 80.9
MsaG034850 MtrunA17_Chr2g0278101 53.933 89 35 2 585 668 173 260 1.12e-16 81.6
MsaG034850 MtrunA17_Chr7g0215531 51.220 82 33 1 531 612 90 164 1.41e-16 77.8
MsaG034850 MtrunA17_Chr8g0391911 28.326 233 129 7 5 211 37 257 4.61e-16 79.0
MsaG034850 MtrunA17_Chr2g0278801 39.695 131 66 3 549 672 121 245 1.04e-15 77.4
MsaG034850 MtrunA17_Chr2g0324891 44.444 90 49 1 110 199 125 213 5.36e-15 77.8
MsaG034850 MtrunA17_Chr4g0040481 59.649 57 23 0 143 199 154 210 1.12e-14 77.0
MsaG034850 MtrunA17_Chr5g0438051 43.810 105 54 2 588 687 175 279 2.45e-14 75.9
MsaG034850 MtrunA17_Chr1g0207991 34.932 146 81 4 549 687 12 150 7.36e-14 72.4
MsaG034850 MtrunA17_Chr5g0400241 53.333 60 28 0 146 205 310 369 8.21e-14 74.7
MsaG034850 MtrunA17_Chr4g0054981 50.794 63 31 0 146 208 309 371 9.77e-14 74.7
MsaG034850 MtrunA17_Chr8g0383961 27.723 202 103 6 5 203 42 203 2.83e-13 70.9
MsaG034850 MtrunA17_Chr7g0235821 36.842 133 69 5 582 712 150 269 3.70e-13 71.6
MsaG034850 MtrunA17_Chr7g0246351 43.689 103 54 2 588 687 177 278 9.27e-13 70.9
MsaG034850 MtrunA17_Chr7g0235851 35.338 133 71 5 582 712 150 269 5.68e-12 68.2
MsaG034850 MtrunA17_Chr5g0417521 54.545 55 24 1 147 200 15 69 2.94e-11 65.9
MsaG034850 MtrunA17_Chr2g0278121 36.923 130 63 4 587 697 184 313 2.99e-11 66.2
MsaG034850 MtrunA17_Chr6g0461641 54.545 55 24 1 147 200 42 96 3.35e-11 65.9
MsaG034850 MtrunA17_Chr3g0091641 30.081 123 83 1 5 124 48 170 3.93e-11 66.2
MsaG034850 MtrunA17_Chr6g0469321 62.791 43 16 0 163 205 70 112 7.24e-11 61.6
MsaG034850 MtrunA17_Chr4g0061021 29.268 123 84 1 5 124 57 179 7.90e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG034850 AT3G16857.1 34.733 262 129 5 5 230 61 316 1.61e-40 158
MsaG034850 AT3G16857.2 34.733 262 129 5 5 230 61 316 1.91e-40 158
MsaG034850 AT3G16857.3 35.060 251 121 5 16 230 2 246 2.12e-39 154
MsaG034850 AT5G07210.2 36.260 262 123 7 4 223 39 298 4.89e-38 150
MsaG034850 AT5G07210.1 36.293 259 121 7 7 223 50 306 1.14e-36 146
MsaG034850 AT2G25180.1 36.916 214 123 2 4 205 40 253 2.91e-35 142
MsaG034850 AT4G31920.1 34.091 220 144 1 4 223 40 258 1.55e-34 139
MsaG034850 AT2G01760.1 36.486 222 118 4 5 203 35 256 2.83e-34 135
MsaG034850 AT2G01760.2 36.036 222 119 4 5 203 63 284 7.05e-33 132
MsaG034850 AT5G58080.1 29.907 321 173 9 4 283 41 350 3.66e-32 133
MsaG034850 AT1G67710.1 35.931 231 127 5 5 214 35 265 1.05e-31 130
MsaG034850 AT2G27070.1 33.205 259 129 5 7 223 45 301 3.24e-31 129
MsaG034850 AT3G62670.1 34.615 208 125 5 3 199 61 268 2.77e-27 115
MsaG034850 AT2G01760.3 36.126 191 99 4 36 203 1 191 3.38e-27 113
MsaG034850 AT5G49240.1 30.986 213 124 3 3 196 64 272 1.76e-26 110
MsaG034850 AT1G67710.2 36.041 197 105 5 39 214 4 200 3.69e-25 109
MsaG034850 AT1G49190.1 31.250 224 129 5 3 204 56 276 1.18e-23 104
MsaG034850 AT1G67710.3 36.667 180 93 5 56 214 12 191 3.71e-21 97.4
MsaG034850 AT5G59570.2 38.053 113 59 2 105 206 88 200 2.04e-18 87.0
MsaG034850 AT5G59570.1 38.053 113 59 2 105 206 88 200 2.04e-18 87.0
MsaG034850 AT3G10760.1 47.297 74 39 0 133 206 91 164 3.54e-18 87.0
MsaG034850 AT2G40970.1 50.725 69 34 0 138 206 96 164 6.88e-18 84.3
MsaG034850 AT5G05090.1 47.222 72 38 0 135 206 69 140 7.45e-18 84.7
MsaG034850 AT3G46640.2 49.296 71 34 1 138 206 133 203 1.80e-17 84.7
MsaG034850 AT3G46640.1 49.296 71 34 1 138 206 133 203 1.80e-17 84.7
MsaG034850 AT3G46640.3 49.296 71 34 1 138 206 133 203 2.90e-17 84.0
MsaG034850 AT5G44190.2 62.295 61 21 1 139 199 141 199 2.51e-16 81.6
MsaG034850 AT5G44190.1 62.295 61 21 1 139 199 141 199 2.62e-16 82.0
MsaG034850 AT2G20570.1 59.259 54 22 0 146 199 152 205 8.23e-14 74.7
MsaG034850 AT4G18020.8 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT4G18020.11 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT4G18020.4 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT4G18020.5 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT4G18020.10 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT4G18020.7 45.333 75 38 1 147 221 296 367 3.78e-13 72.8
MsaG034850 AT2G20570.2 60.784 51 20 0 149 199 171 221 4.12e-13 72.4
MsaG034850 AT4G18020.3 45.333 75 38 1 147 221 296 367 4.26e-13 72.8
MsaG034850 AT4G18020.2 45.333 75 38 1 147 221 296 367 4.26e-13 72.8
MsaG034850 AT4G18020.1 45.333 75 38 1 147 221 296 367 4.26e-13 72.8
MsaG034850 AT4G18020.6 45.333 75 38 1 147 221 296 367 4.26e-13 72.8
MsaG034850 AT4G18020.9 45.333 75 38 1 147 221 296 367 4.26e-13 72.8
MsaG034850 AT1G49190.2 32.653 98 66 0 3 100 56 153 7.08e-12 69.3
MsaG034850 AT3G04450.3 52.632 57 26 1 144 199 235 291 2.22e-11 66.2
MsaG034850 AT3G04450.1 46.479 71 31 2 144 207 235 305 3.45e-11 66.6
MsaG034850 AT3G04450.4 46.479 71 31 2 144 207 195 265 3.73e-11 66.2
MsaG034850 AT3G04450.2 46.479 71 31 2 144 207 195 265 3.73e-11 66.2
MsaG034850 AT5G29000.4 44.286 70 38 1 144 212 228 297 4.35e-11 65.1
MsaG034850 AT3G04030.1 52.727 55 25 1 147 200 45 99 8.94e-11 65.1
MsaG034850 AT3G04030.3 52.727 55 25 1 147 200 45 99 9.95e-11 64.7

Find 116 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACTTCCTCTGAATTTC+TGG 0.123486 7:+1905710 None:intergenic
TGGATTCTATTTCTGATATT+AGG 0.142363 7:-1909888 MsaT034850.1:CDS
TTTGAGTGAGGGTAATATTA+TGG 0.147248 7:+1907646 None:intergenic
CTGAAGACTTCAAAGATATT+TGG 0.172247 7:-1911321 MsaT034850.1:CDS
GTAAGAAACAATAACCATTT+TGG 0.206140 7:-1910087 MsaT034850.1:CDS
AAATTCTAACTTTGGATCTT+TGG 0.211667 7:-1910148 MsaT034850.1:CDS
GAACTATGAATAATGGATTA+AGG 0.228641 7:-1910026 MsaT034850.1:CDS
CAATTCCCTTACATACAAAA+AGG 0.229371 7:-1910444 MsaT034850.1:CDS
TTCTGGGTATGCTCACTTCT+TGG 0.242331 7:+1905727 None:intergenic
ACTTACTTTCCAATCCAATT+TGG 0.246719 7:+1911083 None:intergenic
TAGATCCGAATCGGAGTATT+TGG 0.249167 7:+1907943 None:intergenic
CTGCTTGCTATTAACCAAAT+TGG 0.262572 7:-1911097 MsaT034850.1:CDS
GGATTCTATTTCTGATATTA+GGG 0.272190 7:-1909887 MsaT034850.1:CDS
AACTTGTGAGGCATAATATT+AGG 0.274422 7:+1910120 None:intergenic
TTCTACAGATAGAACCATTC+AGG 0.277804 7:-1907406 MsaT034850.1:CDS
AAATGTAGCAAGTCACTTAC+AGG 0.277878 7:-1910880 MsaT034850.1:intron
GACATTAGGTTTGAACGATA+TGG 0.280554 7:-1907608 MsaT034850.1:CDS
ATGACACACTTACTTATTAT+AGG 0.282733 7:+1912219 None:intergenic
TAAGCTCAGTTATCTTTATA+CGG 0.284237 7:-1907690 MsaT034850.1:intron
CAAAGCATGACGAGCATCTT+TGG 0.288698 7:+1912353 None:intergenic
TTTGGAATTCAGCTGAATAA+TGG 0.297513 7:-1910069 MsaT034850.1:CDS
CAACATGATGCGTTGGAAAT+TGG 0.299414 7:+1910469 None:intergenic
ACTTACTTATTATAGGTATC+TGG 0.300133 7:+1912226 None:intergenic
TATTCAGCTGAATTCCAAAA+TGG 0.301790 7:+1910073 None:intergenic
ATTCAATAATTTGACTCATT+TGG 0.307731 7:-1907276 MsaT034850.1:intron
TTAAGAAACAAAAGCTTGTT+TGG 0.309580 7:-1911144 MsaT034850.1:CDS
GGAATTGGAACTATGAATAA+TGG 0.315352 7:-1910033 MsaT034850.1:CDS
TTGAAGTCTTCAGCACAAAA+AGG 0.324880 7:+1911331 None:intergenic
ATCTCAAAGACACTAGAAAA+TGG 0.331289 7:-1911376 MsaT034850.1:CDS
TTTACTCAACGTGACTTAGA+TGG 0.347030 7:-1909796 MsaT034850.1:CDS
TGCTCTATTCAACTTTGTTA+AGG 0.351980 7:+1907484 None:intergenic
TTCAAGATTGGGAACAAGTT+AGG 0.352069 7:-1910413 MsaT034850.1:CDS
AGATATTTGGAAATATGTTA+TGG 0.355548 7:-1911308 MsaT034850.1:CDS
TTGAGTGAGGGTAATATTAT+GGG 0.358347 7:+1907647 None:intergenic
GGCTGTTCCAAAGAAAATTC+TGG 0.359742 7:-1910934 MsaT034850.1:CDS
AACTATGAATAATGGATTAA+GGG 0.363152 7:-1910025 MsaT034850.1:CDS
TTTCATCTGGTGGGAATCAA+TGG 0.369590 7:-1912282 MsaT034850.1:CDS
AAGGGTTGGCTCTTCAAGAT+TGG 0.372343 7:-1910425 MsaT034850.1:CDS
ACTTGTGAGGCATAATATTA+GGG 0.374491 7:+1910121 None:intergenic
CCCTACATTTGAAAGACATT+AGG 0.378941 7:-1907622 MsaT034850.1:CDS
GGAGCAGAAATGGTTGGAAT+TGG 0.379114 7:-1910048 MsaT034850.1:CDS
TGCTATTAACCAAATTGGAT+TGG 0.390260 7:-1911092 MsaT034850.1:CDS
CGAATCGGAGTATTTGGTTT+CGG 0.397326 7:+1907949 None:intergenic
CGTTATTGCAGAATTTCATC+TGG 0.397811 7:-1912295 MsaT034850.1:CDS
TAAGGTTTCACAGCGTCCTT+TGG 0.408651 7:+1907502 None:intergenic
GGCCATGGTCAAAACAGGTT+TGG 0.410656 7:-1910345 MsaT034850.1:CDS
CCTAATGTCTTTCAAATGTA+GGG 0.413224 7:+1907622 None:intergenic
TAGAGGAGAACAAGGATTGA+TGG 0.421210 7:-1910244 MsaT034850.1:CDS
CTACTTTCACTAATGAGGTT+GGG 0.423849 7:-1909738 MsaT034850.1:CDS
ATCACTCTTCAAGAAAATGA+AGG 0.424779 7:-1912330 MsaT034850.1:CDS
ACCTAATGTCTTTCAAATGT+AGG 0.428587 7:+1907621 None:intergenic
GCTGAATAATGGAGCAGAAA+TGG 0.428747 7:-1910058 MsaT034850.1:CDS
CTGCGAGATGTTGAAGAGAA+AGG 0.429447 7:-1910672 MsaT034850.1:CDS
TCAACTTCCTCTGAATTTCT+GGG 0.430371 7:+1905711 None:intergenic
CAGTGATGTCTGAAGACAAT+AGG 0.437039 7:-1911408 MsaT034850.1:CDS
AGGGTTGGCTCTTCAAGATT+GGG 0.442206 7:-1910424 MsaT034850.1:CDS
GCGTTGGAAATTGGTTGAAA+TGG 0.443167 7:+1910478 None:intergenic
ATAATGGCCATGGTCAAAAC+AGG 0.444362 7:-1910350 MsaT034850.1:CDS
CTTCTTCAAGTTTGTTAACA+AGG 0.448083 7:-1910215 MsaT034850.1:CDS
AGTTGAAGATCTTCAAGAAC+TGG 0.448679 7:+1907557 None:intergenic
GCTACTTTCACTAATGAGGT+TGG 0.473474 7:-1909739 MsaT034850.1:CDS
TGAGTCAAATTATTGAATGT+AGG 0.480068 7:+1907281 None:intergenic
GGTTCAAGGAGGGTTTGCTC+TGG 0.484414 7:-1909908 MsaT034850.1:CDS
GAGTCAAATTATTGAATGTA+GGG 0.484959 7:+1907282 None:intergenic
GCAGGATCAGTTAATAATGA+AGG 0.491730 7:-1909841 MsaT034850.1:CDS
TTCATCTGGTGGGAATCAAT+GGG 0.491841 7:-1912281 MsaT034850.1:CDS
AGTGTCTTTGAGATTAAACT+TGG 0.491853 7:+1911385 None:intergenic
GTTGAAGAGAAAGGAATGGT+TGG 0.491902 7:-1910663 MsaT034850.1:CDS
TCTGATATTAGGGGAGGGAG+TGG 0.493957 7:-1909877 MsaT034850.1:CDS
CTTGAATATCTCTCTCTCCT+TGG 0.495918 7:+1911170 None:intergenic
GATTGGGAACAAGTTAGGCT+TGG 0.497594 7:-1910408 MsaT034850.1:CDS
TAGTGCTACTTTCACTAATG+AGG 0.497764 7:-1909743 MsaT034850.1:CDS
GATGTTATCACTTTCAAATG+TGG 0.498865 7:-1907432 MsaT034850.1:CDS
TTAGTGATATATACCCTGAA+TGG 0.499073 7:+1907392 None:intergenic
TAGGCTTGGAAAATCAAAGT+TGG 0.508790 7:-1910394 MsaT034850.1:CDS
ATTTCTCTGATCACAGAACT+TGG 0.510262 7:+1910594 None:intergenic
GATGTTGTTGCGGAGAATAA+TGG 0.517615 7:-1910366 MsaT034850.1:CDS
TTGTGCTCGCGAAAAGCTGC+TGG 0.522839 7:-1907899 MsaT034850.1:intron
CAGTTATCTTTATACGGATG+AGG 0.523960 7:-1907684 MsaT034850.1:CDS
TCTACAGATAGAACCATTCA+GGG 0.526659 7:-1907405 MsaT034850.1:intron
GAATAAGTGTAGATCCGAAT+CGG 0.532900 7:+1907934 None:intergenic
ATTGCAGAATTTCATCTGGT+GGG 0.533922 7:-1912291 MsaT034850.1:CDS
TTCTTCAAGTTTGTTAACAA+GGG 0.536372 7:-1910214 MsaT034850.1:CDS
ACTTCAATATTTGCAAACAC+TGG 0.542849 7:-1910535 MsaT034850.1:CDS
AGTGGAAGAAAGTTTCTAGC+AGG 0.545083 7:-1909859 MsaT034850.1:CDS
AGCTAGTGGAAGCACCATCA+TGG 0.545478 7:+1910277 None:intergenic
GACTTAGATGGAAATGTTGT+GGG 0.546520 7:-1909784 MsaT034850.1:CDS
TATTGCAGAATTTCATCTGG+TGG 0.554479 7:-1912292 MsaT034850.1:CDS
AATAATGGAGCAGAAATGGT+TGG 0.555811 7:-1910054 MsaT034850.1:CDS
TCCACTCGTCAAAATTCCAA+AGG 0.556141 7:-1907518 MsaT034850.1:CDS
CGAAACCAAATACTCCGATT+CGG 0.558067 7:-1907948 MsaT034850.1:CDS
CTATTTCTGATATTAGGGGA+GGG 0.562145 7:-1909882 MsaT034850.1:CDS
TCTATATGATGAAAAGCTAG+TGG 0.562508 7:+1910263 None:intergenic
AATTTGGTTTGGTTCAAGGA+GGG 0.563542 7:-1909918 MsaT034850.1:CDS
GAAGAGAAAGGAATGGTTGG+AGG 0.566539 7:-1910660 MsaT034850.1:CDS
GATATTATTGATTGTGATTG+CGG 0.570803 7:-1907461 MsaT034850.1:CDS
GTTTGCAAATATTGAAGTGT+TGG 0.578096 7:+1910540 None:intergenic
AATTGGCCAACATGATGCGT+TGG 0.583725 7:+1910462 None:intergenic
TCTATTTCTGATATTAGGGG+AGG 0.587568 7:-1909883 MsaT034850.1:CDS
GAGCATACCCAGAAATTCAG+AGG 0.591726 7:-1905718 MsaT034850.1:CDS
AAATTTGGTTTGGTTCAAGG+AGG 0.591953 7:-1909919 MsaT034850.1:CDS
TCATCATATAGAGGAGAACA+AGG 0.594028 7:-1910252 MsaT034850.1:CDS
TTGCTAATATACTCATGGCA+TGG 0.599578 7:-1915268 None:intergenic
GACATTAGAAACCAGACTCA+TGG 0.599860 7:-1910177 MsaT034850.1:CDS
GATTCTATTTCTGATATTAG+GGG 0.603781 7:-1909886 MsaT034850.1:CDS
AGAAAGAGTAAAGAATGCCA+AGG 0.604346 7:-1911187 MsaT034850.1:CDS
CAATTTCCAACGCATCATGT+TGG 0.607673 7:-1910468 MsaT034850.1:CDS
TGACTTAGATGGAAATGTTG+TGG 0.614635 7:-1909785 MsaT034850.1:CDS
GTTGGAAGCTGATGTTGTTG+CGG 0.645784 7:-1910376 MsaT034850.1:CDS
AAAGTCTTCATACCTTGTTG+CGG 0.645913 7:+1909659 None:intergenic
TGACAAGAATCTCCGCAACA+AGG 0.646714 7:-1909671 MsaT034850.1:intron
CTCATGGCATGGCAATACAA+AGG 0.648754 7:-1915257 MsaT034850.1:intron
ACACTGGCATATCAAACAAG+AGG 0.654807 7:-1910519 MsaT034850.1:CDS
AGATGTTGAAGAGAAAGGAA+TGG 0.660542 7:-1910667 MsaT034850.1:CDS
ACATCAGAAGTAAACTTGTG+AGG 0.669577 7:+1910108 None:intergenic
GTTGCGGAGAATAATGGCCA+TGG 0.690083 7:-1910360 MsaT034850.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 1905680 1915276 1905680 ID=MsaG034850
Chr7 mRNA 1905680 1915276 1905680 ID=MsaT034850.1;Parent=MsaG034850
Chr7 exon 1905680 1905763 1905680 ID=MsaT034850.1.exon9;Parent=MsaT034850.1
Chr7 CDS 1905680 1905763 1905680 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1907277 1907324 1907277 ID=MsaT034850.1.exon8;Parent=MsaT034850.1
Chr7 CDS 1907277 1907324 1907277 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1907406 1907708 1907406 ID=MsaT034850.1.exon7;Parent=MsaT034850.1
Chr7 CDS 1907406 1907708 1907406 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1907900 1907983 1907900 ID=MsaT034850.1.exon6;Parent=MsaT034850.1
Chr7 CDS 1907900 1907983 1907900 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1909672 1910709 1909672 ID=MsaT034850.1.exon5;Parent=MsaT034850.1
Chr7 CDS 1909672 1910709 1909672 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1910881 1910957 1910881 ID=MsaT034850.1.exon4;Parent=MsaT034850.1
Chr7 CDS 1910881 1910957 1910881 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1911089 1911427 1911089 ID=MsaT034850.1.exon3;Parent=MsaT034850.1
Chr7 CDS 1911089 1911427 1911089 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1912232 1912384 1912232 ID=MsaT034850.1.exon2;Parent=MsaT034850.1
Chr7 CDS 1912232 1912384 1912232 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Chr7 exon 1915258 1915276 1915258 ID=MsaT034850.1.exon1;Parent=MsaT034850.1
Chr7 CDS 1915258 1915276 1915258 ID=cds.MsaT034850.1;Parent=MsaT034850.1
Gene Sequence

>MsaT034850.1

ATGGCATGGCAATACAAAGTTGTTACTGCCAAAGATGCTCGTCATGCTTTGATCACTCTTCAAGAAAATGAAGGCTTGTTTGATCTCGTTATTGCAGAATTTCATCTGGTGGGAATCAATGGGTTAGAATTTCAAAAGCATATTAAAGATCAATTCCAGATACCTATAATAATGATGTCTGAAGACAATAGGCCAAGTTTAATCTCAAAGACACTAGAAAATGGAGCTGCACAATATATTGTGAAACCTTTTTGTGCTGAAGACTTCAAAGATATTTGGAAATATGTTATGGAAGCAAAGAAAGAGAAACTTTTCATTGAAAGTTTATTTGTGAGAAGCGAAGAAGAAGAGATATCAAGTGAACCTAAGAACAAAAAGAAGCATTGCAAGAGAAAGAGTAAAGAATGCCAAGGAGAGAGAGATATTCAAGTTGTTAAGAAACAAAAGCTTGTTTGGACACCTTATCTGCACAAAATGTTTCTGCTTGCTATTAACCAAATTGGATTGGAAAAGGCTGTTCCAAAGAAAATTCTGGAGATCATGAATATACCAAACCTGACCAGAGAAAATGTAGCAAGTCACTTACAGAAGTATCGCATATTTCTGCGAGATGTTGAAGAGAAAGGAATGGTTGGAGGCATATCTCACAGAGCTATAAGATCAAGTTTTGCATCAGGTCTACCAAGTTCTGTGATCAGAGAAATCGAAGAAAAAAGGACAAACAAACTTCATGCGCCAACACTTCAATATTTGCAAACACTGGCATATCAAACAAGAGGTGCAAACAATGTTTTTAACCATTTCAACCAATTTCCAACGCATCATGTTGGCCAATTCCCTTACATACAAAAAGGGTTGGCTCTTCAAGATTGGGAACAAGTTAGGCTTGGAAAATCAAAGTTGGAAGCTGATGTTGTTGCGGAGAATAATGGCCATGGTCAAAACAGGTTTGGAATAAGCTCTTCAAACCTTCCATTTTTTCAAGCAAAAAACTCATTCCATGATGGTGCTTCCACTAGCTTTTCATCATATAGAGGAGAACAAGGATTGATGGTTTCTTCTTCTTCAAGTTTGTTAACAAGGGATTTTAAATTTGGTGACATTAGAAACCAGACTCATGGTGTTAAAAATTCTAACTTTGGATCTTTGGCAAGCCCTAATATTATGCCTCACAAGTTTACTTCTGATGTAAGAAACAATAACCATTTTGGAATTCAGCTGAATAATGGAGCAGAAATGGTTGGAATTGGAACTATGAATAATGGATTAAGGGATTCTAGTGCAAATAACAATAGTTTTTGCTTCAACGAAAGGGGTCAAAATGAAAATATAAATTTTAGATCTTTTGTGAATGAAAAATTTGGTTTGGTTCAAGGAGGGTTTGCTCTGGATTCTATTTCTGATATTAGGGGAGGGAGTGGAAGAAAGTTTCTAGCAGGATCAGTTAATAATGAAGGAAATGTTTCAAAGAATAGACAATTTACTCAACGTGACTTAGATGGAAATGTTGTGGGAAATGAGCAAAAGTATAATAGTGCTACTTTCACTAATGAGGTTGGGTTCAACAAAATTGAATTCAACAATTTTGATTACTTAATGGATGATGACAAGAATCTCCGCAACAAGATATCAACTTTACCGAAACCAAATACTCCGATTCGGATCTACACTTATTCGTTGACTCCATCTTGTGCTCGCGAAAAGCTGCTGCTCAGTTATCTTTATACGGATGAGGACTTTTGTTATTATCCCATAATATTACCCTCACTCAAAACCCTACATTTGAAAGACATTAGGTTTGAACGATATGGAGATCTTAAGCGTCTTCTAGAGTATTGTCCAGTTCTTGAAGATCTTCAACTATATGTTGTAATCTTTTCCACTCGTCAAAATTCCAAAGGACGCTGTGAAACCTTAACAAAGTTGAATAGAGCAGATATTATTGATTGTGATTGCGGTGTTCTGATGTTATCACTTTCAAATGTGGAATTTCTACAGATAGAACCATTCAGGGTTTATCTGCATAACGACTTCCCTACATTCAATAATTTGACTCATTTGGGCGCCGAAGAAAACCAAGAAGTGAGCATACCCAGAAATTCAGAGGAAGTTGAATCTTTTTATGGATTTGGCATAACCCTGTAG
Protein sequence

>MsaT034850.1

MAWQYKVVTAKDARHALITLQENEGLFDLVIAEFHLVGINGLEFQKHIKDQFQIPIIMMSEDNRPSLISKTLENGAAQYIVKPFCAEDFKDIWKYVMEAKKEKLFIESLFVRSEEEEISSEPKNKKKHCKRKSKECQGERDIQVVKKQKLVWTPYLHKMFLLAINQIGLEKAVPKKILEIMNIPNLTRENVASHLQKYRIFLRDVEEKGMVGGISHRAIRSSFASGLPSSVIREIEEKRTNKLHAPTLQYLQTLAYQTRGANNVFNHFNQFPTHHVGQFPYIQKGLALQDWEQVRLGKSKLEADVVAENNGHGQNRFGISSSNLPFFQAKNSFHDGASTSFSSYRGEQGLMVSSSSSLLTRDFKFGDIRNQTHGVKNSNFGSLASPNIMPHKFTSDVRNNNHFGIQLNNGAEMVGIGTMNNGLRDSSANNNSFCFNERGQNENINFRSFVNEKFGLVQGGFALDSISDIRGGSGRKFLAGSVNNEGNVSKNRQFTQRDLDGNVVGNEQKYNSATFTNEVGFNKIEFNNFDYLMDDDKNLRNKISTLPKPNTPIRIYTYSLTPSCAREKLLLSYLYTDEDFCYYPIILPSLKTLHLKDIRFERYGDLKRLLEYCPVLEDLQLYVVIFSTRQNSKGRCETLTKLNRADIIDCDCGVLMLSLSNVEFLQIEPFRVYLHNDFPTFNNLTHLGAEENQEVSIPRNSEEVESFYGFGITL*