AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035017

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035017 MtrunA17_Chr7g0214511 84.539 304 24 5 1 298 1 287 3.63e-169 470
MsaG035017 MtrunA17_Chr1g0174001 60.000 195 58 8 113 298 12 195 2.70e-63 198
MsaG035017 MtrunA17_Chr1g0193731 48.680 341 86 17 1 298 1 295 3.34e-61 196
MsaG035017 MtrunA17_Chr1g0169521 50.800 250 78 9 67 298 94 316 9.65e-60 193
MsaG035017 MtrunA17_Chr1g0169561 49.810 263 84 12 55 298 83 316 2.92e-59 192
MsaG035017 MtrunA17_Chr1g0180581 44.561 285 85 12 14 298 9 220 4.87e-49 162
MsaG035017 MtrunA17_Chr1g0174871 38.565 223 103 9 92 297 79 284 2.12e-23 97.1
MsaG035017 MtrunA17_Chr1g0174861 37.989 179 84 9 92 264 67 224 9.73e-21 89.0
MsaG035017 MtrunA17_Chr1g0180571 78.431 51 11 0 243 293 22 72 5.38e-19 81.6
MsaG035017 MtrunA17_Chr1g0172861 35.294 272 97 17 71 298 42 278 1.52e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAAGGTGTTACTGAATT+AGG 0.100666 7:-5948961 MsaT035017.1:CDS
TGAATCACCGTGGCGCTAAA+AGG 0.215611 7:-5949209 MsaT035017.1:CDS
TCGACACGGCCGCCGTTTCT+TGG 0.253917 7:+5949338 None:intergenic
TTCTTGGTGTTGTAGAATAT+TGG 0.276502 7:+5949354 None:intergenic
CTCTTCAATACCATGATTAG+AGG 0.329829 7:+5948993 None:intergenic
ATGAAGGTGTTACTGAATTA+GGG 0.357850 7:-5948960 MsaT035017.1:CDS
ATCGCTCCCTTCAAAATTCC+AGG 0.381362 7:-5949695 MsaT035017.1:CDS
CAAAGCATTGAATGGACATA+AGG 0.388435 7:-5949451 MsaT035017.1:CDS
TATATCTCGCGGAGGTTATG+AGG 0.390309 7:-5949253 MsaT035017.1:CDS
AGTACTTTGAACAGTCAATA+TGG 0.393472 7:+5949382 None:intergenic
AAAACTTTCTTGCAATATGA+TGG 0.405590 7:-5949184 MsaT035017.1:CDS
ATACCATGATTAGAGGTAGA+AGG 0.409626 7:+5949000 None:intergenic
GCATCATTAGTTGTTGAAGA+TGG 0.412853 7:+5949615 None:intergenic
ACTTCTAACAAAGCATTGAA+TGG 0.414800 7:-5949459 MsaT035017.1:CDS
GAATAGGGTTTGAGTAATCA+AGG 0.456225 7:+5949655 None:intergenic
AAAGCATTGAATGGACATAA+GGG 0.470952 7:-5949450 MsaT035017.1:CDS
GAAGATGTTGGTGCAGTTTG+TGG 0.476399 7:+5949417 None:intergenic
GAATCACCGTGGCGCTAAAA+GGG 0.488695 7:-5949208 MsaT035017.1:CDS
TAATCAAGGAAACGGAAAGA+AGG 0.496330 7:+5949669 None:intergenic
GTCAATATGGCTCTGTTCAA+AGG 0.497478 7:+5949395 None:intergenic
TCTTCAACAACTAATGATGC+TGG 0.499426 7:-5949612 MsaT035017.1:CDS
TGATAAACATCATTGGTTTG+TGG 0.499701 7:+5949717 None:intergenic
GGAGCGATGATAAACATCAT+TGG 0.502169 7:+5949710 None:intergenic
GAAGAGAAGAAGATTAATGA+AGG 0.510676 7:-5948976 MsaT035017.1:CDS
TCAAACATCACAAGATTGCA+AGG 0.513757 7:-5949025 MsaT035017.1:CDS
AAGATGTTGGTGCAGTTTGT+GGG 0.516549 7:+5949418 None:intergenic
GAACCTTCTACCTCTAATCA+TGG 0.517420 7:-5949003 MsaT035017.1:CDS
TTGGTGTTGTAGAATATTGG+CGG 0.527171 7:+5949357 None:intergenic
TTGAGGTTCAAGATTCCTAA+AGG 0.528982 7:-5949057 MsaT035017.1:CDS
GAAACGGAAAGAAGGGTACC+TGG 0.540917 7:+5949677 None:intergenic
TAGCCACAATGAAAAGAAGT+TGG 0.545096 7:-5949082 MsaT035017.1:CDS
CTCATGTTATGTTTGCAACA+AGG 0.547505 7:-5949487 MsaT035017.1:CDS
GGTTTGAGTAATCAAGGAAA+CGG 0.550662 7:+5949661 None:intergenic
TTCTGTTCTTGGTAATCACA+AGG 0.559776 7:-5949544 MsaT035017.1:CDS
TATTCTACAACACCAAGAAA+CGG 0.569291 7:-5949350 MsaT035017.1:CDS
TCATGATCATGAGAAGGAGA+TGG 0.576941 7:+5949741 None:intergenic
AATCAAGGAAACGGAAAGAA+GGG 0.583538 7:+5949670 None:intergenic
TCATCTTCAAAATCATCACT+TGG 0.584649 7:+5948901 None:intergenic
CAAGGTGTGTTTCTAACAAG+TGG 0.594003 7:-5949132 MsaT035017.1:CDS
ATGAAAAGAAGTTGGTGTTG+AGG 0.594249 7:-5949074 MsaT035017.1:CDS
ACAACGTGAAGAAGATCGTA+TGG 0.595118 7:-5949578 MsaT035017.1:CDS
TGTGGTTCATGATCATGAGA+AGG 0.604571 7:+5949735 None:intergenic
ATATCTCGCGGAGGTTATGA+GGG 0.614101 7:-5949252 MsaT035017.1:CDS
CTCTGTTCAAAGGAAGATGT+TGG 0.645368 7:+5949405 None:intergenic
ACTTTGTTGCATATATCTCG+CGG 0.675938 7:-5949264 MsaT035017.1:CDS
TCTACAACACCAAGAAACGG+CGG 0.738098 7:-5949347 MsaT035017.1:CDS
TTGTTGCATATATCTCGCGG+AGG 0.742758 7:-5949261 MsaT035017.1:CDS
AATATCGATGTGAATCACCG+TGG 0.797456 7:-5949219 MsaT035017.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 5948891 5949787 5948891 ID=MsaG035017
Chr7 mRNA 5948891 5949787 5948891 ID=MsaT035017.1;Parent=MsaG035017
Chr7 exon 5948891 5949787 5948891 ID=MsaT035017.1.exon1;Parent=MsaT035017.1
Chr7 CDS 5948891 5949787 5948891 ID=cds.MsaT035017.1;Parent=MsaT035017.1
Gene Sequence

>MsaT035017.1

ATGTATTCTTCTTCTTCTTCATCTCCATCTCCTTCTCATGATCATGAACCACAAACCAATGATGTTTATCATCGCTCCCTTCAAAATTCCAGGTACCCTTCTTTCCGTTTCCTTGATTACTCAAACCCTATTCAGAATGAACTCAAAAATCCATCTTCAACAACTAATGATGCTGGAAAACAACAGAACAACGTGAAGAAGATCGTATGGTTTTTTTCTTATTCTGTTCTTGGTAATCACAAGGATGTTGAAAAACAGAACAAACATACATACTCATACTCATGTTATGTTTGCAACAAGGTTTTTACTTCTAACAAAGCATTGAATGGACATAAGGGTGTTATTCACCCACAAACTGCACCAACATCTTCCTTTGAACAGAGCCATATTGACTGTTCAAAGTACTTGCCGCCAATATTCTACAACACCAAGAAACGGCGGCCGTGTCGAAGAAGAAGAAGAAGATATATGAACATTGATGATGATGTGATTGCTGCTGAAACTTTGTTGCATATATCTCGCGGAGGTTATGAGGGATCATCTGTCAATATCGATGTGAATCACCGTGGCGCTAAAAGGGAAAAACTTTCTTGCAATATGATGGATGATGATGAGAAGAAGAAGAAGAAGGAACAAGGTGTGTTTCTAACAAGTGGCGATATTGATGAAACATTGAAAGATAATAGCCACAATGAAAAGAAGTTGGTGTTGAGGTTCAAGATTCCTAAAGGCAAGATTTTTCAAACATCACAAGATTGCAAGGAACCTTCTACCTCTAATCATGGTATTGAAGAGAAGAAGATTAATGAAGGTGTTACTGAATTAGGGTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTGATGATTTTGAAGATGAAACTAATTAG
Protein sequence

>MsaT035017.1

MYSSSSSSPSPSHDHEPQTNDVYHRSLQNSRYPSFRFLDYSNPIQNELKNPSSTTNDAGKQQNNVKKIVWFFSYSVLGNHKDVEKQNKHTYSYSCYVCNKVFTSNKALNGHKGVIHPQTAPTSSFEQSHIDCSKYLPPIFYNTKKRRPCRRRRRRYMNIDDDVIAAETLLHISRGGYEGSSVNIDVNHRGAKREKLSCNMMDDDEKKKKKEQGVFLTSGDIDETLKDNSHNEKKLVLRFKIPKGKIFQTSQDCKEPSTSNHGIEEKKINEGVTELGSKVVKNFDLNELPSDDFEDETN*