AlfalfaGEDB | MsaG035018

M. sativa ssp. caerulea / MsaG035018

KEGG: K02218
GO: -
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035018	XP_003621013.1	97.902	572	8	1	1	568	1	572	0.0	1168

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035018	sp\|Q852L0\|HD16N_ORYSJ	76.678	566	126	3	7	568	117	680	0.0	927

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035018	tr\|G7KWQ1\|G7KWQ1_MEDTR	97.902	572	8	1	1	568	1	572	0.0	1168

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002214	MsaG035018	0.841164	1.039939e-57	4.211178e-55
MsaG003422	MsaG035018	0.809887	2.589595e-50	4.410226e-48
MsaG003427	MsaG035018	0.803639	5.332967e-49	7.833811e-47
MsaG003536	MsaG035018	0.841556	8.208784e-58	3.365741e-55
MsaG003768	MsaG035018	0.803526	5.629094e-49	8.247315e-47
MsaG003966	MsaG035018	0.801064	1.798276e-48	2.490386e-46
MsaG005107	MsaG035018	0.806593	1.293725e-49	2.036128e-47
MsaG005496	MsaG035018	0.802048	1.132796e-48	1.604232e-46
MsaG005497	MsaG035018	0.818346	3.587307e-52	7.554507e-50
MsaG005510	MsaG035018	0.809051	3.906629e-50	6.520470e-48
MsaG006170	MsaG035018	0.827710	2.404093e-54	6.521120e-52
MsaG006452	MsaG035018	0.818643	3.073383e-52	6.522495e-50
MsaG006555	MsaG035018	0.817095	6.848288e-52	1.396499e-49
MsaG006623	MsaG035018	0.819907	1.589289e-52	3.486737e-50
MsaG006855	MsaG035018	0.801437	1.509655e-48	2.108492e-46
MsaG006988	MsaG035018	0.803795	4.951902e-49	7.300563e-47
MsaG009767	MsaG035018	0.808523	5.059019e-50	8.337216e-48
MsaG015184	MsaG035018	0.816180	1.095467e-51	2.181972e-49
MsaG015722	MsaG035018	0.807511	8.286668e-50	1.332818e-47
MsaG016389	MsaG035018	0.804007	4.477441e-49	6.633664e-47
MsaG017303	MsaG035018	0.815990	1.207299e-51	2.393116e-49
MsaG018078	MsaG035018	0.801051	1.809070e-48	2.504618e-46
MsaG018786	MsaG035018	0.816409	9.740985e-52	1.951573e-49
MsaG018793	MsaG035018	0.839329	3.117799e-57	1.191687e-54
MsaG019564	MsaG035018	0.802818	7.873821e-49	1.134895e-46
MsaG019910	MsaG035018	0.809135	3.748256e-50	6.268833e-48
MsaG020371	MsaG035018	0.801178	1.704965e-48	2.367293e-46
MsaG020420	MsaG035018	0.800862	1.976517e-48	2.724881e-46
MsaG020467	MsaG035018	0.809679	2.868181e-50	4.860245e-48
MsaG020474	MsaG035018	0.806633	1.268973e-49	1.999047e-47
MsaG023005	MsaG035018	0.808637	4.785235e-50	7.907493e-48
MsaG023623	MsaG035018	0.847938	1.592691e-59	8.049186e-57
MsaG023959	MsaG035018	0.823981	1.828753e-53	4.474057e-51
MsaG026478	MsaG035018	0.806903	1.113202e-49	1.764774e-47
MsaG026841	MsaG035018	0.811468	1.183045e-50	2.094092e-48
MsaG026849	MsaG035018	0.818190	3.889381e-52	8.157916e-50
MsaG030039	MsaG035018	0.800685	2.147684e-48	2.949149e-46
MsaG034946	MsaG035018	0.810052	2.386303e-50	4.080253e-48
MsaG035018	MsaG040093	0.809223	3.589466e-50	6.015661e-48
MsaG035018	MsaG041721	0.801093	1.774216e-48	2.458710e-46
MsaG035018	MsaG041931	0.821170	8.175885e-53	1.854769e-50
MsaG035018	MsaG042579	0.805884	1.821664e-49	2.819276e-47
MsaG035018	MsaG042717	0.823924	1.884846e-53	4.604234e-51
MsaG035018	MsaG045439	0.803371	6.057899e-49	8.843912e-47
MsaG035018	MsaG045568	0.830627	4.751890e-55	1.400042e-52
MsaG035018	MsaG045699	0.806468	1.374455e-49	2.156835e-47
MsaG035018	MsaG045769	0.845557	7.079366e-59	3.303953e-56
MsaG035018	MsaG046104	0.826123	5.735444e-54	1.488314e-51
MsaG035018	MsaG046497	0.803571	5.510705e-49	8.082183e-47
MsaG035018	MsaG046946	0.817154	6.644093e-52	1.356902e-49
MsaG035018	MsaG047011	0.836716	1.455405e-56	5.132647e-54
MsaG035018	MsaG001564	0.833113	1.164455e-55	3.688352e-53
MsaG035018	MsaG002467	0.819138	2.375791e-52	5.107920e-50
MsaG035018	MsaG037798	0.809186	3.655377e-50	6.120781e-48
MsaG035018	MsaG037545	0.800209	2.681666e-48	3.642804e-46
MsaG035018	MsaG038853	0.808033	6.428876e-50	1.047052e-47
MsaG035018	MsaG035795	0.840333	1.712359e-57	6.755052e-55

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035018	MtrunA17_Chr7g0214531	97.902	572	8	1	1	568	1	572	0.0	1168
MsaG035018	MtrunA17_Chr1g0213321	83.333	576	83	3	1	568	79	649	0.0	1022
MsaG035018	MtrunA17_Chr1g0146301	77.408	571	122	3	3	568	89	657	0.0	948
MsaG035018	MtrunA17_Chr3g0140431	76.450	569	128	2	4	568	5	571	0.0	942
MsaG035018	MtrunA17_Chr7g0253211	78.881	554	112	2	7	556	294	846	0.0	939
MsaG035018	MtrunA17_Chr8g0340491	72.352	557	147	4	6	556	97	652	0.0	848
MsaG035018	MtrunA17_Chr2g0329981	39.636	275	153	5	37	308	9	273	1.16e-60	208
MsaG035018	MtrunA17_Chr4g0071031	39.273	275	154	5	37	308	9	273	1.20e-60	207
MsaG035018	MtrunA17_Chr2g0299661	38.545	275	156	5	37	308	9	273	3.57e-60	207
MsaG035018	MtrunA17_Chr1g0166821	39.298	285	158	6	31	312	6	278	8.60e-60	205
MsaG035018	MtrunA17_Chr7g0261501	38.710	279	158	5	37	312	9	277	3.28e-59	204
MsaG035018	MtrunA17_Chr1g0187571	38.947	285	159	6	31	312	6	278	3.10e-58	201
MsaG035018	MtrunA17_Chr1g0183631	37.895	285	162	6	31	312	5	277	8.52e-58	200
MsaG035018	MtrunA17_Chr8g0342821	37.818	275	157	6	37	308	9	272	1.11e-57	200
MsaG035018	MtrunA17_Chr6g0486611	36.786	280	164	5	37	313	9	278	2.66e-56	195
MsaG035018	MtrunA17_Chr8g0390211	37.586	290	165	8	27	313	2	278	9.89e-56	194
MsaG035018	MtrunA17_Chr1g0167261	37.024	289	165	6	29	312	4	280	1.70e-54	188
MsaG035018	MtrunA17_Chr1g0146151	35.943	281	165	6	31	308	5	273	1.73e-53	187
MsaG035018	MtrunA17_Chr3g0140071	35.587	281	166	6	31	308	5	273	1.76e-53	187

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035018	AT3G13670.1	83.333	576	86	3	1	568	103	676	0.0	1025
MsaG035018	AT2G25760.1	75.652	575	127	5	3	568	76	646	0.0	937
MsaG035018	AT2G25760.2	75.433	578	126	7	3	568	76	649	0.0	930
MsaG035018	AT5G18190.2	76.492	553	127	1	7	556	100	652	0.0	922
MsaG035018	AT5G18190.1	76.492	553	127	1	7	556	100	652	0.0	922
MsaG035018	AT3G03940.1	74.690	565	140	1	7	568	110	674	0.0	918
MsaG035018	AT3G03930.1	63.598	239	78	2	339	568	23	261	2.11e-103	314
MsaG035018	AT2G19470.1	39.068	279	157	5	37	312	9	277	2.99e-60	206
MsaG035018	AT5G44100.1	38.596	285	160	6	31	312	5	277	9.27e-60	206
MsaG035018	AT5G44100.2	38.596	285	160	6	31	312	5	277	9.27e-60	206
MsaG035018	AT1G03930.1	38.790	281	157	6	31	308	5	273	1.25e-59	206
MsaG035018	AT3G23340.1	38.947	285	159	6	31	312	5	277	2.29e-59	204
MsaG035018	AT4G14340.2	38.596	285	160	6	31	312	11	283	5.98e-59	201
MsaG035018	AT4G26100.1	37.634	279	161	5	37	312	9	277	7.65e-59	203
MsaG035018	AT4G28540.1	38.246	285	161	6	31	312	9	281	1.01e-58	203
MsaG035018	AT4G14340.1	38.596	285	160	6	31	312	11	283	1.82e-58	202
MsaG035018	AT4G28860.2	38.062	289	163	7	27	312	2	277	7.32e-58	198
MsaG035018	AT4G28860.1	38.408	289	162	8	27	312	2	277	2.48e-57	198
MsaG035018	AT1G72710.1	38.182	275	156	6	37	308	9	272	4.47e-57	199
MsaG035018	AT4G28880.3	38.754	289	161	8	27	312	2	277	5.14e-57	197
MsaG035018	AT4G28880.2	38.754	289	161	8	27	312	2	277	5.14e-57	197
MsaG035018	AT4G28880.1	38.754	289	161	8	27	312	2	277	5.14e-57	197
MsaG035018	AT4G28880.4	38.754	289	161	8	27	312	2	277	5.14e-57	197
MsaG035018	AT5G57015.1	36.559	279	164	5	37	312	9	277	7.04e-57	197
MsaG035018	AT1G04440.1	38.246	285	161	6	31	312	5	277	1.05e-56	197
MsaG035018	AT5G43320.1	38.246	285	161	6	31	312	5	277	1.61e-56	197
MsaG035018	AT5G43320.2	38.246	285	161	6	31	312	5	277	1.84e-56	194
MsaG035018	AT5G43320.3	38.246	285	161	6	31	312	5	277	1.84e-56	194
MsaG035018	AT3G23340.2	38.346	266	156	4	50	312	3	263	6.41e-53	186
MsaG035018	AT4G08800.1	32.975	279	142	6	37	312	9	245	4.93e-43	155

Find 153 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATCTCTGGGCACTTATTA+TGG	0.176547	7:+5968875	MsaT035018.1:CDS
CTTCCTTTATAATGTACTTT+AGG	0.233162	7:-5964966	None:intergenic
CATCTCTACCTTTGCTTCAT+TGG	0.235235	7:-5967854	None:intergenic
TGGCTTCTTGTTCCAATCTC+TGG	0.237696	7:+5968862	MsaT035018.1:CDS
AAAAGAAACCTGAACATATC+AGG	0.258582	7:-5967092	None:intergenic
TGAGCACCTTCAGTATTAAT+TGG	0.274374	7:-5967594	None:intergenic
GGTGGTAATGACCGTCTTAA+TGG	0.277319	7:+5964605	MsaT035018.1:CDS
AAACTTGGCAAAGGTGGATT+TGG	0.289497	7:+5964554	MsaT035018.1:CDS
TGCAAGTGTTCATGCTCATT+TGG	0.304706	7:+5967193	MsaT035018.1:CDS
AACAGTAAAGGTTGCAACTA+TGG	0.325296	7:+5964754	MsaT035018.1:CDS
GTTACCATGGCAAGGTTATC+AGG	0.332076	7:+5967286	MsaT035018.1:CDS
TTTCTTGTTGATCTCGGATT+AGG	0.333361	7:+5966935	MsaT035018.1:CDS
GTCCAAGCTTGTGGGATGTT+TGG	0.336979	7:+5965118	MsaT035018.1:CDS
TCTATAGCTGGGGTTACTAA+TGG	0.339143	7:+5969094	MsaT035018.1:CDS
CTTATTATGGATGCTGGAAC+TGG	0.352603	7:+5968888	MsaT035018.1:CDS
GGCTTCTTGTTCCAATCTCT+GGG	0.354817	7:+5968863	MsaT035018.1:CDS
TTTGTTGGTCGTCGAGTAAC+CGG	0.359465	7:+5964584	MsaT035018.1:CDS
TTCTTCTCTTGTGCTGTACC+TGG	0.364237	7:-5966908	None:intergenic
ATTGGCCGCAGTGCAGGATT+TGG	0.366177	7:-5967576	None:intergenic
CATAATAAGTGCCCAGAGAT+TGG	0.371273	7:-5968874	None:intergenic
GGCGATGGGATAATGGTTAC+AGG	0.374463	7:+5970861	MsaT035018.1:CDS
AGCAATGCATGCCACCATTT+CGG	0.376237	7:-5966697	None:intergenic
ATTTCCAAGCACACATGTTA+AGG	0.378927	7:+5970983	MsaT035018.1:CDS
GGCCACTGCTGGAAGTCGTT+GGG	0.383031	7:+5969936	MsaT035018.1:CDS
GCAAGTGTTCATGCTCATTT+GGG	0.388698	7:+5967194	MsaT035018.1:CDS
ATCCATAGGCGATGGGATAA+TGG	0.393182	7:+5970854	MsaT035018.1:CDS
CAGAAGCAGCATAGCATCTC+AGG	0.394079	7:-5967450	None:intergenic
CTTCTTGTTTATCCATTTGA+AGG	0.400317	7:-5969884	None:intergenic
TATTACATTACTTCTATAGC+TGG	0.401862	7:+5969082	MsaT035018.1:CDS
ATACCTAAAGTACATTATAA+AGG	0.411632	7:+5964963	MsaT035018.1:CDS
GATATTAGCTTGGAATAGTT+AGG	0.422192	7:-5967531	None:intergenic
ATTGATATCCATTGTGTAGC+AGG	0.425137	7:-5967815	None:intergenic
ACACATACCCTCTCAGGAAA+AGG	0.427758	7:-5963572	None:intergenic
GATAACCTTGCCATGGTAAC+CGG	0.435532	7:-5967283	None:intergenic
CTATAGCTGGGGTTACTAAT+GGG	0.437207	7:+5969095	MsaT035018.1:CDS
ACCAGCGCCCTGATATGTTC+AGG	0.437919	7:+5967084	MsaT035018.1:CDS
TTCAAAGTGGAGAGGAAACT+TGG	0.443586	7:+5964539	MsaT035018.1:CDS
GCGAGTCTTTCCCCTTCAAA+TGG	0.445977	7:+5969872	MsaT035018.1:CDS
CACATACCCTCTCAGGAAAA+GGG	0.446418	7:-5963571	None:intergenic
GTATTAATTGGCCGCAGTGC+AGG	0.451258	7:-5967582	None:intergenic
TCTCTTGTGGTGATGTCAAA+AGG	0.451724	7:+5969121	MsaT035018.1:CDS
TAATACTGAAGGTGCTCAAA+AGG	0.460450	7:+5967599	MsaT035018.1:CDS
TCCTTGTTTGATAGTGTGCT+TGG	0.461993	7:+5967552	MsaT035018.1:CDS
AACCATTATCCCATCGCCTA+TGG	0.469884	7:-5970856	None:intergenic
TGTGGAGTCATTGTCGATCT+TGG	0.469974	7:+5966719	MsaT035018.1:CDS
AAATTGTTTCTTGTTGATCT+CGG	0.470903	7:+5966929	MsaT035018.1:CDS
TTCACAGCAGGTCTTCGCCT+TGG	0.472756	7:-5970935	None:intergenic
TTCATTATAGATATGTGCAT+GGG	0.475655	7:+5966852	MsaT035018.1:intron
GTGACTTCAATGGCCACTGC+TGG	0.476110	7:+5969925	MsaT035018.1:CDS
ATGTAGGATGTCTGCCGAAA+TGG	0.477227	7:+5966683	MsaT035018.1:intron
ATTACATTACTTCTATAGCT+GGG	0.477779	7:+5969083	MsaT035018.1:CDS
GGAATGGATCATGGAGCAAT+GGG	0.480666	7:+5969051	MsaT035018.1:intron
GTTGTTATGTCACGGAATGC+TGG	0.481715	7:+5969961	MsaT035018.1:CDS
GAGAGGTAGATTGAATATTG+AGG	0.484728	7:+5967748	MsaT035018.1:CDS
TGGCAAAGGTGGATTTGGAC+AGG	0.492641	7:+5964559	MsaT035018.1:CDS
TTCCAAACATCCCACAAGCT+TGG	0.493712	7:-5965120	None:intergenic
CATAGGACACATACCCTCTC+AGG	0.494559	7:-5963578	None:intergenic
CGCCTATGGATTCCCTCACT+AGG	0.503575	7:-5970842	None:intergenic
ATCAAACAAGGATATTAGCT+TGG	0.504809	7:-5967541	None:intergenic
TGGCCACTGCTGGAAGTCGT+TGG	0.506701	7:+5969935	MsaT035018.1:CDS
TGTGAAACTCAGGAAACTCT+TGG	0.507507	7:+5970952	MsaT035018.1:CDS
AGGAAATGTCTGAAAGGAGT+AGG	0.507896	7:-5967474	None:intergenic
GAATGCTGGATTCAGTGATC+AGG	0.508380	7:+5969975	MsaT035018.1:CDS
CATGCTTGGTCCAAGCTTGT+GGG	0.512086	7:+5965110	MsaT035018.1:CDS
TTCTTGTTTATCCATTTGAA+GGG	0.516197	7:-5969883	None:intergenic
TTCCTCTCCACTTTGAACAT+AGG	0.519828	7:-5964533	None:intergenic
AGCACCCTTGGTGGAAGTCA+TGG	0.521686	7:+5964939	MsaT035018.1:intron
TGGGCACTTATTATGGATGC+TGG	0.523125	7:+5968882	MsaT035018.1:CDS
TCACAGCAACTGCAGCTACA+TGG	0.524987	7:+5970885	MsaT035018.1:CDS
CTAACCTTAACATGTGTGCT+TGG	0.525324	7:-5970987	None:intergenic
AGGTGTTGCTCAAGAGGATG+AGG	0.525431	7:+5963538	MsaT035018.1:CDS
TCAAATGGATAAACAAGAAG+TGG	0.528244	7:+5969887	MsaT035018.1:CDS
TTCTTTATAGGAATGGATCA+TGG	0.534028	7:+5969042	MsaT035018.1:intron
CGGCCTTCTTATTAGTTGTG+TGG	0.534547	7:+5968842	MsaT035018.1:CDS
ACCTGAACATATCAGGGCGC+TGG	0.539965	7:-5967085	None:intergenic
GGTGGTTTGAGTGCTAATAA+AGG	0.540456	7:+5963518	MsaT035018.1:CDS
AGGAATGGATCATGGAGCAA+TGG	0.542705	7:+5969050	MsaT035018.1:intron
TAATAAAGGTGTTGCTCAAG+AGG	0.543294	7:+5963532	MsaT035018.1:CDS
TACTAATGGGAGCTCTCTTG+TGG	0.544290	7:+5969108	MsaT035018.1:CDS
TTCATAAAGGCCGGTTACCA+TGG	0.544662	7:+5967273	MsaT035018.1:CDS
GTGAGGGAATCCATAGGCGA+TGG	0.545096	7:+5970846	MsaT035018.1:CDS
ATGCAGGTTATGGACATGCT+TGG	0.546212	7:+5965096	MsaT035018.1:intron
GACCCAAAGGTTCAAGTAGG+AGG	0.548405	7:+5964506	MsaT035018.1:intron
GAAGACCTGCTGTGAAACTC+AGG	0.549057	7:+5970942	MsaT035018.1:CDS
TTTGAACATAGAAACAGTAA+AGG	0.551586	7:+5964742	MsaT035018.1:CDS
TGGGATGTTTGGAATACCAC+AGG	0.555511	7:+5965129	MsaT035018.1:CDS
AAGCCACACAACTAATAAGA+AGG	0.555747	7:-5968845	None:intergenic
TCTTGGAGAAGATGCACTCA+AGG	0.557515	7:+5966736	MsaT035018.1:CDS
CACAGCAACTGCAGCTACAT+GGG	0.559415	7:+5970886	MsaT035018.1:CDS
GAGAGGAAACTTGGCAAAGG+TGG	0.562906	7:+5964548	MsaT035018.1:CDS
GTTCATTATAGATATGTGCA+TGG	0.563871	7:+5966851	MsaT035018.1:intron
AGTTTCCTGAGTTTCACAGC+AGG	0.564323	7:-5970947	None:intergenic
ACATGCTTGGTCCAAGCTTG+TGG	0.566277	7:+5965109	MsaT035018.1:CDS
ATTAAGACGGTCATTACCAC+CGG	0.568388	7:-5964603	None:intergenic
TTGTACACTTGCCACTCGTA+AGG	0.569716	7:-5964781	None:intergenic
GGATTCCCTCACTAGGGTAG+AGG	0.571629	7:-5970835	None:intergenic
TACACTTGCCACTCGTAAGG+AGG	0.576490	7:-5964778	None:intergenic
ATAAACAAGAAGTGGAGAGA+AGG	0.576509	7:+5969895	MsaT035018.1:CDS
TTTGACCCAAAGGTTCAAGT+AGG	0.576801	7:+5964503	MsaT035018.1:intron
TGTTTGGAATACCACAGGCC+AGG	0.580670	7:+5965134	MsaT035018.1:CDS
AACTCAAGGAAATGTCTGAA+AGG	0.580963	7:-5967480	None:intergenic
GTTGGGGTGTTGTTATGTCA+CGG	0.581602	7:+5969953	MsaT035018.1:CDS
TCATGTTCACTACAAACTCA+AGG	0.582669	7:-5967494	None:intergenic
GTGTTCATGCTCATTTGGGA+AGG	0.584104	7:+5967198	MsaT035018.1:CDS
GGGGTGATGGGTGATGAAAG+TGG	0.587284	7:+5963497	None:intergenic
TATCATTACAATGTAGCGGA+TGG	0.588065	7:+5968783	MsaT035018.1:CDS
CAAGAATGCCAATGAAGCAA+AGG	0.595613	7:+5967846	MsaT035018.1:CDS
GGAAATGTCTGAAAGGAGTA+GGG	0.595669	7:-5967473	None:intergenic
TGATTTCCTCTACCCTAGTG+AGG	0.596385	7:+5970829	MsaT035018.1:CDS
CTTGGAGAAGATGCACTCAA+GGG	0.598220	7:+5966737	MsaT035018.1:CDS
GATTTCCTCTACCCTAGTGA+GGG	0.598868	7:+5970830	MsaT035018.1:CDS
CTGCGGCCAATTAATACTGA+AGG	0.599700	7:+5967588	MsaT035018.1:CDS
AAGTGGAGAGACACCTCCAC+TGG	0.602028	7:+5967044	MsaT035018.1:CDS
AAAGAAACCTGAACATATCA+GGG	0.602522	7:-5967091	None:intergenic
GCCTATGGATTCCCTCACTA+GGG	0.605702	7:-5970841	None:intergenic
TACCAGGTCGGGCAAAAGAG+AGG	0.609022	7:+5967731	MsaT035018.1:intron
TGAGGGAATCCATAGGCGAT+GGG	0.614592	7:+5970847	MsaT035018.1:CDS
TCTTGTTTATCCATTTGAAG+GGG	0.615159	7:-5969882	None:intergenic
CGCCTATGTTCAAAGTGGAG+AGG	0.617102	7:+5964531	MsaT035018.1:CDS
TATTCCATGACTTCCACCAA+GGG	0.620145	7:-5964943	None:intergenic
ACCCCAACGACTTCCAGCAG+TGG	0.622356	7:-5969938	None:intergenic
TTATACTCACGCCTGGCCTG+TGG	0.625190	7:-5965145	None:intergenic
TTCAACATGCTGACCAGTGG+AGG	0.626398	7:-5967057	None:intergenic
ACCCTAGTGAGGGAATCCAT+AGG	0.629058	7:+5970840	MsaT035018.1:CDS
GTGGCACTGGGCCATTAAGA+CGG	0.629072	7:-5964616	None:intergenic
TTACATTACTTCTATAGCTG+GGG	0.629898	7:+5969084	MsaT035018.1:CDS
ATATTCAACATGCTGACCAG+TGG	0.633992	7:-5967060	None:intergenic
ACATACCCTCTCAGGAAAAG+GGG	0.634258	7:-5963570	None:intergenic
AGCACCTGATAACCTTGCCA+TGG	0.635229	7:-5967290	None:intergenic
TTGGAGAAGATGCACTCAAG+GGG	0.638893	7:+5966738	MsaT035018.1:CDS
AAAGGCCGGTTACCATGGCA+AGG	0.640441	7:+5967278	MsaT035018.1:CDS
ACAAGGAGAATATTACGTGA+TGG	0.641400	7:+5964989	MsaT035018.1:CDS
ACACTTGCCACTCGTAAGGA+GGG	0.643172	7:-5964777	None:intergenic
GTAGAGCGAGGGATTGCAGA+CGG	0.643300	7:+5968822	MsaT035018.1:CDS
CTCTTATATTAAGCAAACCA+AGG	0.643646	7:+5970918	MsaT035018.1:CDS
GCCACTGCTGGAAGTCGTTG+GGG	0.646395	7:+5969937	MsaT035018.1:CDS
TTAGCGCAGCATGTAGAGCG+AGG	0.648996	7:+5968810	MsaT035018.1:CDS
TCCAAGCACACTATCAAACA+AGG	0.651722	7:-5967553	None:intergenic
GTGGAGAGGAAACTTGGCAA+AGG	0.654106	7:+5964545	MsaT035018.1:CDS
TAGCGCAGCATGTAGAGCGA+GGG	0.654538	7:+5968811	MsaT035018.1:CDS
TAGGATGTCTGCCGAAATGG+TGG	0.657696	7:+5966686	MsaT035018.1:intron
GTACATTATAAAGGAAGACA+AGG	0.658821	7:+5964972	MsaT035018.1:CDS
GTTGGTCGTCGAGTAACCGG+TGG	0.658919	7:+5964587	MsaT035018.1:CDS
GGTGTTGCTCAAGAGGATGA+GGG	0.659331	7:+5963539	MsaT035018.1:CDS
TAGGTATCATTACAATGTAG+CGG	0.659418	7:+5968779	MsaT035018.1:intron
AATGGTGGCATGCATTGCTG+TGG	0.668208	7:+5966701	MsaT035018.1:CDS
GTATTCCATGACTTCCACCA+AGG	0.671722	7:-5964944	None:intergenic
AGGTTCGCCTATGTTCAAAG+TGG	0.676871	7:+5964526	MsaT035018.1:CDS
ACTATGGCCCTCCTTACGAG+TGG	0.689741	7:+5964770	MsaT035018.1:CDS
TGGAGAAGATGCACTCAAGG+GGG	0.711646	7:+5966739	MsaT035018.1:CDS
TCATTATAGATATGTGCATG+GGG	0.716228	7:+5966853	MsaT035018.1:intron
GTGATGGGTGATGAAAGTGG+TGG	0.743174	7:+5963500	None:intergenic
TTGGACCAAATCCTGCACTG+CGG	0.818000	7:+5967571	MsaT035018.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	5963503	5971003	5963503	ID=MsaG035018
Chr7	mRNA	5963503	5971003	5963503	ID=MsaT035018.1;Parent=MsaG035018
Chr7	exon	5963503	5963586	5963503	ID=MsaT035018.1.exon1;Parent=MsaT035018.1
Chr7	CDS	5963503	5963586	5963503	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5964515	5964631	5964515	ID=MsaT035018.1.exon2;Parent=MsaT035018.1
Chr7	CDS	5964515	5964631	5964515	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5964730	5964803	5964730	ID=MsaT035018.1.exon3;Parent=MsaT035018.1
Chr7	CDS	5964730	5964803	5964730	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5964941	5965010	5964941	ID=MsaT035018.1.exon4;Parent=MsaT035018.1
Chr7	CDS	5964941	5965010	5964941	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5965102	5965157	5965102	ID=MsaT035018.1.exon5;Parent=MsaT035018.1
Chr7	CDS	5965102	5965157	5965102	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5966689	5966760	5966689	ID=MsaT035018.1.exon6;Parent=MsaT035018.1
Chr7	CDS	5966689	5966760	5966689	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5966862	5966956	5966862	ID=MsaT035018.1.exon7;Parent=MsaT035018.1
Chr7	CDS	5966862	5966956	5966862	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5967039	5967105	5967039	ID=MsaT035018.1.exon8;Parent=MsaT035018.1
Chr7	CDS	5967039	5967105	5967039	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5967178	5967307	5967178	ID=MsaT035018.1.exon9;Parent=MsaT035018.1
Chr7	CDS	5967178	5967307	5967178	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5967402	5967620	5967402	ID=MsaT035018.1.exon10;Parent=MsaT035018.1
Chr7	CDS	5967402	5967620	5967402	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5967737	5967867	5967737	ID=MsaT035018.1.exon11;Parent=MsaT035018.1
Chr7	CDS	5967737	5967867	5967737	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5968782	5968956	5968782	ID=MsaT035018.1.exon12;Parent=MsaT035018.1
Chr7	CDS	5968782	5968956	5968782	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5969052	5969142	5969052	ID=MsaT035018.1.exon13;Parent=MsaT035018.1
Chr7	CDS	5969052	5969142	5969052	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5969839	5969996	5969839	ID=MsaT035018.1.exon14;Parent=MsaT035018.1
Chr7	CDS	5969839	5969996	5969839	ID=cds.MsaT035018.1;Parent=MsaT035018.1
Chr7	exon	5970818	5971003	5970818	ID=MsaT035018.1.exon15;Parent=MsaT035018.1
Chr7	CDS	5970818	5971003	5970818	ID=cds.MsaT035018.1;Parent=MsaT035018.1

Gene Sequence

>MsaT035018.1

ATGGGTGATGAAAGTGGTGGTTTGAGTGCTAATAAAGGTGTTGCTCAAGAGGATGAGGGCAATACTACCCCTTTTCCTGAGAGGGTTCAAGTAGGAGGTTCGCCTATGTTCAAAGTGGAGAGGAAACTTGGCAAAGGTGGATTTGGACAGGTTTTTGTTGGTCGTCGAGTAACCGGTGGTAATGACCGTCTTAATGGCCCAGTGGCTTTGAAATTTGAACATAGAAACAGTAAAGGTTGCAACTATGGCCCTCCTTACGAGTGGCAAGTGTACAACACCCTTGGTGGAAGTCATGGAATACCTAAAGTACATTATAAAGGAAGACAAGGAGAATATTACGTGATGGTTATGGACATGCTTGGTCCAAGCTTGTGGGATGTTTGGAATACCACAGGCCAGGCGATGTCTGCCGAAATGGTGGCATGCATTGCTGTGGAGTCATTGTCGATCTTGGAGAAGATGCACTCAAGGGGATATGTGCATGGGGATGTAAAACCAGAGAACTTTTTACTAGGTCAGCCAGGTACAGCACAAGAGAAGAAATTGTTTCTTGTTGATCTCGGATTAGCAACAAAGTGGAGAGACACCTCCACTGGTCAGCATGTTGAATATGACCAGCGCCCTGATATGTTCAGAGGAACTGTTCGATATGCAAGTGTTCATGCTCATTTGGGAAGGACTGCTAGCAGAAGAGATGATCTTGAATCTCTTGCATATACTCTCATTTTTCTTCATAAAGGCCGGTTACCATGGCAAGGTTATCAGGGAGATAATAAGTCGTATCTTGTTTGCAAAAAGAAGATGGGAACATCTCCTGAGATGCTATGCTGCTTCTGCCCTACTCCTTTCAGACATTTCCTTGAGTTTGTAGTGAACATGAAATTCGATGAAGAGCCTAACTATTCCAAGCTAATATCCTTGTTTGATAGTGTGCTTGGACCAAATCCTGCACTGCGGCCAATTAATACTGAAGGTGCTCAAAAGGTCGGGCAAAAGAGAGGTAGATTGAATATTGAGGAAGAAGATGATTCGCAGCCTAAAAAGAAGGTTCGTTTGGGGGTTCCTGCTACACAATGGATATCAATTTACAATGCAAGAATGCCAATGAAGCAAAGGTATCATTACAATGTAGCGGATGGACGATTAGCGCAGCATGTAGAGCGAGGGATTGCAGACGGCCTTCTTATTAGTTGTGTGGCTTCTTGTTCCAATCTCTGGGCACTTATTATGGATGCTGGAACTGGATTTACAAATCAAGTTTACAAGTTGTCACCTTTTTTCTTACACAAGGAATGGATCATGGAGCAATGGGAGAAGAACTATTACATTACTTCTATAGCTGGGGTTACTAATGGGAGCTCTCTTGTGGTGATGTCAAAAGGCACACAGTATACACAACAATCATACAAAGTAAGCGAGTCTTTCCCCTTCAAATGGATAAACAAGAAGTGGAGAGAAGGTTTTCACGTGACTTCAATGGCCACTGCTGGAAGTCGTTGGGGTGTTGTTATGTCACGGAATGCTGGATTCAGTGATCAGGTTGTTGAACTTGATTTCCTCTACCCTAGTGAGGGAATCCATAGGCGATGGGATAATGGTTACAGGATCACAGCAACTGCAGCTACATGGGATCAATCTGCTCTTATATTAAGCAAACCAAGGCGAAGACCTGCTGTGAAACTCAGGAAACTCTTGGACATCTCAATTTCCAAGCACACATGTTAA

Protein sequence

>MsaT035018.1

MGDESGGLSANKGVAQEDEGNTTPFPERVQVGGSPMFKVERKLGKGGFGQVFVGRRVTGGNDRLNGPVALKFEHRNSKGCNYGPPYEWQVYNTLGGSHGIPKVHYKGRQGEYYVMVMDMLGPSLWDVWNTTGQAMSAEMVACIAVESLSILEKMHSRGYVHGDVKPENFLLGQPGTAQEKKLFLVDLGLATKWRDTSTGQHVEYDQRPDMFRGTVRYASVHAHLGRTASRRDDLESLAYTLIFLHKGRLPWQGYQGDNKSYLVCKKKMGTSPEMLCCFCPTPFRHFLEFVVNMKFDEEPNYSKLISLFDSVLGPNPALRPINTEGAQKVGQKRGRLNIEEEDDSQPKKKVRLGVPATQWISIYNARMPMKQRYHYNVADGRLAQHVERGIADGLLISCVASCSNLWALIMDAGTGFTNQVYKLSPFFLHKEWIMEQWEKNYYITSIAGVTNGSSLVVMSKGTQYTQQSYKVSESFPFKWINKKWREGFHVTSMATAGSRWGVVMSRNAGFSDQVVELDFLYPSEGIHRRWDNGYRITATAATWDQSALILSKPRRRPAVKLRKLLDISISKHTC*