AlfalfaGEDB | MsaG035055

M. sativa ssp. caerulea / MsaG035055

KEGG: K11446
GO: GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010605 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0099402 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
PFAM: PF02373.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035055	RHN78372.1	89.080	174	19	0	1	174	167	340	2.94e-109	341

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035055	sp\|Q336N8\|JM706_ORYSJ	69.753	162	49	0	1	162	280	441	5.65e-76	254

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035055	tr\|A0A396JLW8\|A0A396JLW8_MEDTR	89.080	174	19	0	1	174	167	340	1.40e-109	341

TFs/TRs:

Gene ID	Type	Classification
MsaG035055	TR	Jumonji

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035055	MtrunA17_Chr1g0164761	89.080	174	19	0	1	174	167	340	2.70e-113	341
MsaG035055	MtrunA17_Chr1g0164731	86.286	175	24	0	1	175	222	396	1.52e-110	335
MsaG035055	MtrunA17_Chr5g0410721	69.697	165	50	0	1	165	266	430	1.58e-80	263
MsaG035055	MtrunA17_Chr4g0039841	75.595	168	15	4	196	339	201	366	5.84e-65	213
MsaG035055	MtrunA17_Chr7g0214951	83.621	116	1	2	1	115	103	201	7.20e-61	195
MsaG035055	MtrunA17_Chr5g0395341	60.000	155	47	5	196	338	173	324	9.31e-47	164
MsaG035055	MtrunA17_Chr1g0199511	44.516	155	82	1	1	155	324	474	2.56e-44	163
MsaG035055	MtrunA17_Chr3g0116441	44.505	182	87	3	1	174	205	380	8.67e-43	159
MsaG035055	MtrunA17_Chr5g0397501	44.022	184	87	4	2	179	418	591	1.21e-38	147
MsaG035055	MtrunA17_Chr8g0380981	52.143	140	60	2	2	141	285	417	1.79e-37	143
MsaG035055	MtrunA17_Chr2g0303101	40.099	202	103	4	2	196	294	484	2.44e-37	143
MsaG035055	MtrunA17_Chr1g0189211	45.455	154	77	2	2	155	391	537	1.03e-36	141
MsaG035055	MtrunA17_Chr7g0261401	36.025	161	85	3	2	155	368	517	3.76e-24	104

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035055	AT5G46910.2	65.946	185	61	1	1	185	280	462	1.90e-83	266
MsaG035055	AT5G46910.1	65.946	185	61	1	1	185	280	462	1.42e-82	267
MsaG035055	AT1G63490.1	45.078	193	82	2	2	177	160	345	6.58e-46	168
MsaG035055	AT1G63490.4	45.078	193	82	2	2	177	413	598	1.13e-45	168
MsaG035055	AT1G63490.2	45.078	193	82	2	2	177	413	598	1.16e-45	168
MsaG035055	AT5G16880.4	57.229	166	43	6	196	336	173	335	3.77e-44	157
MsaG035055	AT5G16880.1	57.229	166	43	6	196	336	173	335	3.77e-44	157
MsaG035055	AT5G16880.2	57.229	166	43	6	196	336	173	335	3.77e-44	157
MsaG035055	AT5G04240.1	46.452	155	79	1	1	155	292	442	6.16e-44	163
MsaG035055	AT3G48430.1	40.933	193	100	3	1	185	233	419	2.17e-41	155
MsaG035055	AT4G20400.1	44.654	159	81	2	2	160	297	448	1.16e-36	141
MsaG035055	AT4G20400.2	44.654	159	81	2	2	160	240	391	1.46e-36	141
MsaG035055	AT2G34880.1	42.941	170	90	2	2	171	295	457	3.05e-36	140
MsaG035055	AT1G30810.3	44.720	161	76	2	2	159	295	445	3.13e-36	140
MsaG035055	AT1G30810.2	44.720	161	76	2	2	159	295	445	3.13e-36	140
MsaG035055	AT1G30810.1	44.720	161	76	2	2	159	295	445	3.13e-36	140
MsaG035055	AT1G08620.3	45.455	154	77	2	2	155	409	555	2.79e-34	134
MsaG035055	AT1G08620.2	45.455	154	77	2	2	155	395	541	2.86e-34	134
MsaG035055	AT1G08620.4	45.455	154	77	2	2	155	395	541	2.86e-34	134
MsaG035055	AT1G08620.1	45.455	154	77	2	2	155	395	541	2.86e-34	134
MsaG035055	AT5G16880.3	57.143	119	31	5	196	297	173	288	2.74e-28	112
MsaG035055	AT1G63490.3	46.400	125	50	1	70	177	15	139	3.51e-27	113
MsaG035055	AT2G38950.1	35.065	154	93	2	2	155	322	468	1.02e-20	94.4
MsaG035055	AT1G06210.1	34.899	149	72	6	205	334	179	321	7.70e-11	63.5

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTTCAACTCTTCATATT+TGG	0.134665	7:+6660176	None:intergenic
AATCCGGCATGATATGAATT+TGG	0.274912	7:+6664927	None:intergenic
GGAGAGTGAGGAGTTATAAT+AGG	0.288102	7:+6660723	None:intergenic
TTGATGATTGGTTTCCATTC+GGG	0.289403	7:-6664309	MsaT035055.1:CDS
AAAGCTTCCTTTGTTTGTTC+AGG	0.294751	7:+6660630	None:intergenic
TTGTTCAATAACTGGAGTTC+AGG	0.304605	7:-6660057	MsaT035055.1:CDS
AGTTCCTCATATGGAATTAT+AGG	0.316333	7:+6664247	None:intergenic
TTACAAAGAAGTTCCTCATA+TGG	0.344918	7:+6664238	None:intergenic
GGTTCAAGTGGAGAAGCTTT+AGG	0.347577	7:+6660150	None:intergenic
CTCTACATTGGGATGCTATT+CGG	0.371427	7:-6665330	MsaT035055.1:CDS
GTTTGAAATCAAGGGGCATT+TGG	0.373941	7:-6660776	MsaT035055.1:CDS
TAAACTTTGCTATTGATGAT+TGG	0.380245	7:-6664321	MsaT035055.1:CDS
TGATTCTATGGATGGTAGAT+TGG	0.381924	7:+6660274	None:intergenic
AAGCCAGCTTGTTCAATAAC+TGG	0.386018	7:-6660065	MsaT035055.1:CDS
ATTGATGATTGGTTTCCATT+CGG	0.386443	7:-6664310	MsaT035055.1:CDS
CAACAGTCTCAATGATTCTA+TGG	0.389732	7:+6660262	None:intergenic
CCTATCATCATATCCGGTTC+TGG	0.393270	7:+6660129	None:intergenic
ACCTCAGCAAGATGTTCTTA+AGG	0.393840	7:-6660550	MsaT035055.1:intron
CTAAAATGAGGTGACTCATC+TGG	0.421690	7:+6660091	None:intergenic
TCAATTTGAAAAGACTGTCT+TGG	0.425142	7:-6665111	MsaT035055.1:CDS
TTCTCCACTTGAACCAGAAC+CGG	0.425437	7:-6660142	MsaT035055.1:CDS
TAATTAACCTCTATCTTTCG+AGG	0.430616	7:+6664192	None:intergenic
CAAAATCAATGGAGCTGAGA+TGG	0.439728	7:-6661994	MsaT035055.1:CDS
TGTTGCAGAGTTTGAAATCA+AGG	0.441356	7:-6660785	MsaT035055.1:intron
GATTCTATGGATGGTAGATT+GGG	0.453613	7:+6660275	None:intergenic
GCTACCTATAATTCCATATG+AGG	0.458631	7:-6664251	MsaT035055.1:CDS
TTAATAAAACATGGAGAGAA+TGG	0.461249	7:-6665065	MsaT035055.1:CDS
CCAGAACCGGATATGATGAT+AGG	0.469919	7:-6660129	MsaT035055.1:CDS
TGTTCAATAACTGGAGTTCA+GGG	0.471455	7:-6660056	MsaT035055.1:CDS
GAGAGTACCATGACTAAATC+CGG	0.473300	7:+6664911	None:intergenic
AATTGAGAGGCTGCAGAACT+AGG	0.476304	7:+6665128	None:intergenic
TACAAGGTGGTGCAAAAGCC+AGG	0.481676	7:-6664969	MsaT035055.1:CDS
TCAAGGGGCATTTGGTTCCC+TGG	0.484301	7:-6660768	MsaT035055.1:CDS
TCATCTGGCTCAATCGTAAC+CGG	0.488704	7:+6660106	None:intergenic
GGAGAACGTCTCAAAATCAA+TGG	0.490004	7:-6662005	MsaT035055.1:CDS
CTGATCCTATGCTCTACATT+GGG	0.491971	7:-6665341	None:intergenic
TACTATGAGTTTCAAGATGA+AGG	0.493177	7:-6661946	MsaT035055.1:CDS
GTTTGTTCAGGCTTGAGACT+CGG	0.497817	7:+6660642	None:intergenic
TACAACTCCTCGAAAGATAG+AGG	0.500398	7:-6664199	MsaT035055.1:intron
AGTGCAAATAAAACTTGGTA+TGG	0.503293	7:-6665155	MsaT035055.1:CDS
TGCTATTCGGTATGTTTGCA+TGG	0.504172	7:-6665317	MsaT035055.1:CDS
ACATAATGTGCCGGTTTACA+AGG	0.506507	7:-6664985	MsaT035055.1:CDS
AACCGGCCTATCATCATATC+CGG	0.511490	7:+6660123	None:intergenic
TAGAAGATCACTATTTGTAC+AGG	0.521776	7:-6665290	MsaT035055.1:intron
AAGCTTTCGTTGTTACGACC+AGG	0.522685	7:+6660750	None:intergenic
TGTAAATGATGAGATTCATA+AGG	0.524735	7:-6660205	MsaT035055.1:CDS
GCAAGACTGTGATCAATCTC+TGG	0.527784	7:+6660693	None:intergenic
TCGCTAGCTGCAGCCCCGAA+TGG	0.538348	7:+6664295	None:intergenic
ACTGATCCTATGCTCTACAT+TGG	0.538565	7:-6665342	None:intergenic
TTATGTTCTAACAAGACATC+AGG	0.538881	7:+6665002	None:intergenic
TGATGATTGGTTTCCATTCG+GGG	0.543433	7:-6664308	MsaT035055.1:CDS
GTTGCAGAGTTTGAAATCAA+GGG	0.543815	7:-6660784	MsaT035055.1:intron
CTTGTTAGAACATAATGTGC+CGG	0.547471	7:-6664994	MsaT035055.1:CDS
CATAGAATCATTGAGACTGT+TGG	0.550434	7:-6660261	MsaT035055.1:CDS
TTTCCAAATTCATATCATGC+CGG	0.551032	7:-6664930	MsaT035055.1:CDS
ACTCAAGTGCAAATAAAACT+TGG	0.552288	7:-6665160	MsaT035055.1:CDS
TGATCAATCTCTGGTGCAGA+GGG	0.553009	7:+6660702	None:intergenic
TGGTCGTAACAACGAAAGCT+TGG	0.553081	7:-6660748	MsaT035055.1:CDS
TTCAGGCTTGAGACTCGGCA+CGG	0.554954	7:+6660647	None:intergenic
AGTCTCAATGATTCTATGGA+TGG	0.556941	7:+6660266	None:intergenic
AGCTGGCTTTCTCTAAAATG+AGG	0.560727	7:+6660079	None:intergenic
CAAGACATCAGGAGAGAACA+TGG	0.563327	7:+6665013	None:intergenic
AATCTCATCATTTACATGCA+AGG	0.566421	7:+6660212	None:intergenic
AAAATCAATGGAGCTGAGAT+GGG	0.573637	7:-6661993	MsaT035055.1:CDS
AAGGTGATAACAAATTCTCC+TGG	0.578556	7:+6664951	None:intergenic
TCTGGTGCAGAGGGAGAGTG+AGG	0.581678	7:+6660711	None:intergenic
ACTCCAGTTATTGAACAAGC+TGG	0.582394	7:+6660062	None:intergenic
TCAGGCTTGAGACTCGGCAC+GGG	0.582998	7:+6660648	None:intergenic
AACCGGATATGATGATAGGC+CGG	0.586672	7:-6660125	MsaT035055.1:CDS
GTGATCAATCTCTGGTGCAG+AGG	0.590336	7:+6660701	None:intergenic
TATCATGCCGGATTTAGTCA+TGG	0.595929	7:-6664918	MsaT035055.1:intron
TTGCAGAGTTTGAAATCAAG+GGG	0.603635	7:-6660783	MsaT035055.1:intron
GGATGGTAGATTGGGACTGA+TGG	0.607628	7:+6660283	None:intergenic
AGCTTTCGTTGTTACGACCA+GGG	0.609472	7:+6660751	None:intergenic
AACAAAATCTTAATAAAACA+TGG	0.617591	7:-6665074	MsaT035055.1:CDS
TCTCAAGCCTGAACAAACAA+AGG	0.625648	7:-6660637	MsaT035055.1:CDS
ATATCCGGTTCTGGTTCAAG+TGG	0.631567	7:+6660138	None:intergenic
AGCATCCCAATGTAGAGCAT+AGG	0.635201	7:+6665336	None:intergenic
ACCTTAAGAACATCTTGCTG+AGG	0.639392	7:+6660549	None:intergenic
GGCATGATATGAATTTGGAA+AGG	0.649539	7:+6664932	None:intergenic
TAATGTGCCGGTTTACAAGG+TGG	0.674784	7:-6664982	MsaT035055.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	6660044	6665355	6660044	ID=MsaG035055
Chr7	mRNA	6660044	6665355	6660044	ID=MsaT035055.1;Parent=MsaG035055
Chr7	exon	6660044	6660310	6660044	ID=MsaT035055.1.exon6;Parent=MsaT035055.1
Chr7	CDS	6660044	6660310	6660044	ID=cds.MsaT035055.1;Parent=MsaT035055.1
Chr7	exon	6660551	6660797	6660551	ID=MsaT035055.1.exon5;Parent=MsaT035055.1
Chr7	CDS	6660551	6660797	6660551	ID=cds.MsaT035055.1;Parent=MsaT035055.1
Chr7	exon	6661945	6662026	6661945	ID=MsaT035055.1.exon4;Parent=MsaT035055.1
Chr7	CDS	6661945	6662026	6661945	ID=cds.MsaT035055.1;Parent=MsaT035055.1
Chr7	exon	6664200	6664364	6664200	ID=MsaT035055.1.exon3;Parent=MsaT035055.1
Chr7	CDS	6664200	6664364	6664200	ID=cds.MsaT035055.1;Parent=MsaT035055.1
Chr7	exon	6664919	6665196	6664919	ID=MsaT035055.1.exon2;Parent=MsaT035055.1
Chr7	CDS	6664919	6665196	6664919	ID=cds.MsaT035055.1;Parent=MsaT035055.1
Chr7	exon	6665291	6665355	6665291	ID=MsaT035055.1.exon1;Parent=MsaT035055.1
Chr7	CDS	6665291	6665355	6665291	ID=cds.MsaT035055.1;Parent=MsaT035055.1

Gene Sequence

>MsaT035055.1

ATGCTCTACATTGGGATGCTATTCGGTATGTTTGCATGGCATGTAGAAGATCACTATTTGTACAGTATAAATTACCATCACTCAAGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGGATCATGTGTATTGCAACAAAATCTTAATAAAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGTGGTGCAAAAGCCAGGAGAATTTGTTATCACCTTTCCAAATTCATATCATGCCGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGATGATTGGTTTCCATTCGGGGCTGCAGCTAGCGAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCGAAAGATAGAGGAGAACGTCTCAAAATCAATGGAGCTGAGATGGGTCGAAAAATGAGTGCTGGAATAGGTACTATGAGTTTCAAGATGAAGGATTTGAAATCAAGGGGCATTTGGTTCCCTGGTCGTAACAACGAAAGCTTGGCTCCTATTATAACTCCTCACTCTCCCTCTGCACCAGAGATTGATCACAGTCTTGCACATCTTATTCAGTGTGAAAATCCCGTGCCGAGTCTCAAGCCTGAACAAACAAAGGAAGCTTTTGATGTTGCAAGAAACAGCACTGAGACTGAGCTTCTCTCTTCTGTGTTATCTTCTTCACCTCAGCAAGATGTTCTTAAGCAATGCCATCAGTCCCAATCTACCATCCATAGAATCATTGAGACTGTTGGCGAAAATGAAGCATTGCTTTTTGAGGCCTTGCATGTAAATGATGAGATTCATAAGGTTCTCTCCAAATATGAAGAGTTGAAGAAGAAGCCTAAAGCTTCTCCACTTGAACCAGAACCGGATATGATGATAGGCCGGTTACGATTGAGCCAGATGAGTCACCTCATTTTAGAGAAAGCCAGCTTGTTCAATAACTGGAGTTCAGGGATTGAGCCATGA

Protein sequence

>MsaT035055.1

MLYIGMLFGMFAWHVEDHYLYSINYHHSSANKTWYGVPSSAASQFEKTVLDHVYCNKILIKHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKVVQKPGEFVITFPNSYHAGFSHGFNCGEAVNFAIDDWFPFGAAASERYSHLKMLPIIPYEELLCKEALLIYNSSKDRGERLKINGAEMGRKMSAGIGTMSFKMKDLKSRGIWFPGRNNESLAPIITPHSPSAPEIDHSLAHLIQCENPVPSLKPEQTKEAFDVARNSTETELLSSVLSSSPQQDVLKQCHQSQSTIHRIIETVGENEALLFEALHVNDEIHKVLSKYEELKKKPKASPLEPEPDMMIGRLRLSQMSHLILEKASLFNNWSSGIEP*