AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035056


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035056 MtrunA17_Chr5g0445971 82.114 123 20 1 1 123 1 121 1.20e-67 205
MsaG035056 MtrunA17_Chr5g0435401 75.556 90 22 0 34 123 44 133 2.47e-41 144
MsaG035056 MtrunA17_Chr5g0445951 72.131 61 16 1 123 182 74 134 4.61e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCAAACTCACTCAGCTTT+TGG 0.244787 7:+6670748 MsaT035056.1:CDS
CACCTTCTTGTAGGAACATC+TGG 0.271791 7:-6672165 None:intergenic
AGTTGTTTCTGCACTAAAAC+TGG 0.324340 7:+6671411 MsaT035056.1:CDS
AGGTTGGCCATGAAAAGAAA+TGG 0.416893 7:+6671383 MsaT035056.1:intron
TAGAAGGTCTCCAAGTCATT+AGG 0.434040 7:-6670725 None:intergenic
CTGAGTGAGTTTGACGTAGA+AGG 0.450835 7:-6670741 None:intergenic
GGTTGGCCATGAAAAGAAAT+GGG 0.469245 7:+6671384 MsaT035056.1:intron
GTACTTCTACAGATCACAAA+AGG 0.488119 7:+6671507 MsaT035056.1:CDS
CTCAATGGAAGACAAGGAAA+TGG 0.517620 7:+6670634 None:intergenic
ACCAGTGTTGTCTTGTCCGA+CGG 0.530488 7:+6670686 MsaT035056.1:CDS
TATTGAATCAAAAGAAGCTA+AGG 0.535783 7:-6672100 None:intergenic
CCAGTGTTGTCTTGTCCGAC+GGG 0.549085 7:+6670687 MsaT035056.1:CDS
GTATTGTTCTCCTAATGACT+TGG 0.554561 7:+6670715 MsaT035056.1:CDS
ACACTGGTGAGTTGTGTGTT+CGG 0.557134 7:-6670671 None:intergenic
ACAAGTCTAGGGATAGTTCT+CGG 0.558393 7:-6670964 None:intergenic
ACCCAGATGTTCCTACAAGA+AGG 0.578630 7:+6672163 MsaT035056.1:CDS
GTTTCTGCACTAAAACTGGA+AGG 0.584593 7:+6671415 MsaT035056.1:CDS
ACCTTCTTGTAGGAACATCT+GGG 0.599072 7:-6672164 None:intergenic
TACACCCATCTACAAAGGCA+TGG 0.605358 7:-6672413 None:intergenic
TTACAGAGATGACAATGCAA+TGG 0.612606 7:-6672062 None:intergenic
ACACAACTCAATGGAAGACA+AGG 0.625634 7:+6670628 None:intergenic
CTATAAGTCAAATATTAGCA+AGG 0.635319 7:+6672315 MsaT035056.1:CDS
AAGGATGTAGTGAGAGAACT+CGG 0.640037 7:+6672334 MsaT035056.1:CDS
GGCATTACACCCATCTACAA+AGG 0.641850 7:-6672418 None:intergenic
TGTAGTGAGAGAACTCGGTA+CGG 0.654606 7:+6672339 MsaT035056.1:CDS
CAAGGAAATGGTGAAGACGA+CGG 0.673865 7:+6670646 MsaT035056.1:CDS
TTGGCCATGAAAAGAAATGG+GGG 0.692749 7:+6671386 MsaT035056.1:CDS
CCCGTCGGACAAGACAACAC+TGG 0.699697 7:-6670687 None:intergenic
GTTGGCCATGAAAAGAAATG+GGG 0.704590 7:+6671385 MsaT035056.1:CDS
AGAACAATACTGTTTCCCGT+CGG 0.725382 7:-6670702 None:intergenic
TGGCCATGAAAAGAAATGGG+GGG 0.738244 7:+6671387 MsaT035056.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 6670638 6672426 6670638 ID=MsaG035056
Chr7 mRNA 6670638 6672426 6670638 ID=MsaT035056.1;Parent=MsaG035056
Chr7 exon 6670638 6670792 6670638 ID=MsaT035056.1.exon1;Parent=MsaT035056.1
Chr7 CDS 6670638 6670792 6670638 ID=cds.MsaT035056.1;Parent=MsaT035056.1
Chr7 exon 6670955 6671030 6670955 ID=MsaT035056.1.exon2;Parent=MsaT035056.1
Chr7 CDS 6670955 6671030 6670955 ID=cds.MsaT035056.1;Parent=MsaT035056.1
Chr7 exon 6671385 6671528 6671385 ID=MsaT035056.1.exon3;Parent=MsaT035056.1
Chr7 CDS 6671385 6671528 6671385 ID=cds.MsaT035056.1;Parent=MsaT035056.1
Chr7 exon 6672048 6672184 6672048 ID=MsaT035056.1.exon4;Parent=MsaT035056.1
Chr7 CDS 6672048 6672184 6672048 ID=cds.MsaT035056.1;Parent=MsaT035056.1
Chr7 exon 6672312 6672426 6672312 ID=MsaT035056.1.exon5;Parent=MsaT035056.1
Chr7 CDS 6672312 6672426 6672312 ID=cds.MsaT035056.1;Parent=MsaT035056.1
Gene Sequence

>MsaT035056.1

ATGGAAGACAAGGAAATGGTGAAGACGACGGTTCCGAACACACAACTCACCAGTGTTGTCTTGTCCGACGGGAAACAGTATTGTTCTCCTAATGACTTGGAGACCTTCTACGTCAAACTCACTCAGCTTTTGGACTTGCTTGGATTCACTCTCATTTTCAATGTCCGAGAACTATCCCTAGACTTGTACGTCTTTTTCTTGGAGGTCACAAAAAGAGGAGGTTATCACCAGGTTGGCCATGAAAAGAAATGGGGGGAAGTTGTTTCTGCACTAAAACTGGAAGGAAACAATGCAGTCTTATGTGCTCAAGTTGAAAAACTCTATGGTTATCTTCTTTACGAATTTGAGAAATTGTACTTCTACAGATCACAAAAGTTCTGCAATGCAAACCATTGCATTGTCATCTCTGTAAGCAATTTTCTGAAAACCTTAGCTTCTTTTGATTCAATACTTCTTTGTATGAATTCTATCTTTTTACAGGGAAGATGCTACCCAGATGTTCCTACAAGAAGATTCTATAAGTCAAATATTAGCAAGGATGTAGTGAGAGAACTCGGTACGGAATGTAAAGATTTTTGGCAATGTGTTTTATTTGTTGGTACTTTGTGTCTGTCCATGCCTTTGTAG

Protein sequence

>MsaT035056.1

MEDKEMVKTTVPNTQLTSVVLSDGKQYCSPNDLETFYVKLTQLLDLLGFTLIFNVRELSLDLYVFFLEVTKRGGYHQVGHEKKWGEVVSALKLEGNNAVLCAQVEKLYGYLLYEFEKLYFYRSQKFCNANHCIVISVSNFLKTLASFDSILLCMNSIFLQGRCYPDVPTRRFYKSNISKDVVRELGTECKDFWQCVLFVGTLCLSMPL*