AlfalfaGEDB | MsaG035081

M. sativa ssp. caerulea / MsaG035081

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009986 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010154 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0080167 GO:0090558 GO:0090567 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000012
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035081	XP_003630022.1	91.710	193	4	2	1	186	1	188	6.53e-110	332

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035081	sp\|O64682\|PID_ARATH	69.189	185	44	6	8	186	6	183	7.66e-77	239

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035081	tr\|G7L9M4\|G7L9M4_MEDTR	91.710	193	4	2	1	186	1	188	3.12e-110	332

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035081	MtrunA17_Chr8g0381091	91.710	193	4	2	1	186	1	188	6.00e-114	332
MsaG035081	MtrunA17_Chr2g0285711	74.747	198	32	6	1	186	1	192	1.33e-88	266
MsaG035081	MtrunA17_Chr4g0063311	51.825	137	63	2	52	186	66	201	4.07e-45	155
MsaG035081	MtrunA17_Chr1g0179101	51.908	131	59	2	45	175	438	564	6.65e-41	146
MsaG035081	MtrunA17_Chr2g0290691	51.538	130	59	2	52	181	179	304	3.56e-39	140
MsaG035081	MtrunA17_Chr2g0312641	49.265	136	65	3	52	186	67	199	3.67e-38	136
MsaG035081	MtrunA17_Chr7g0266031	51.613	124	56	2	52	175	467	586	9.40e-38	137
MsaG035081	MtrunA17_Chr8g0335351	48.148	135	66	2	52	186	345	475	8.44e-37	135
MsaG035081	MtrunA17_Chr2g0309421	48.462	130	62	2	48	177	147	271	2.83e-36	132
MsaG035081	MtrunA17_Chr3g0080101	46.667	135	68	2	52	186	390	520	9.88e-36	132
MsaG035081	MtrunA17_Chr7g0250631	48.148	135	66	2	52	186	339	469	1.31e-35	131
MsaG035081	MtrunA17_Chr7g0227331	48.148	135	66	3	52	186	207	337	3.18e-35	130
MsaG035081	MtrunA17_Chr6g0449401	43.787	169	84	4	12	176	197	358	8.88e-35	129
MsaG035081	MtrunA17_Chr8g0356291	45.588	136	71	1	51	186	100	232	5.25e-34	125
MsaG035081	MtrunA17_Chr8g0369091	36.735	147	89	2	40	186	560	702	2.90e-26	104
MsaG035081	MtrunA17_Chr2g0321101	40.152	132	78	1	56	186	66	197	5.95e-26	103
MsaG035081	MtrunA17_Chr2g0327561	36.296	135	82	2	52	186	601	731	1.28e-22	94.4
MsaG035081	MtrunA17_Chr4g0030361	36.296	135	82	2	52	186	282	412	1.58e-22	94.0
MsaG035081	MtrunA17_Chr7g0234641	31.356	118	75	2	74	186	7	123	3.70e-15	72.4
MsaG035081	MtrunA17_Chr8g0338741	32.743	113	67	2	71	175	11	122	1.73e-14	70.5
MsaG035081	MtrunA17_Chr2g0329011	31.818	110	71	2	75	184	12	117	1.26e-13	68.2
MsaG035081	MtrunA17_Chr4g0032761	33.333	105	66	2	75	175	10	114	6.03e-13	66.2
MsaG035081	MtrunA17_Chr1g0197721	30.435	115	70	2	54	168	32	136	2.44e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035081	AT2G34650.1	69.189	185	44	6	8	186	6	183	7.79e-78	239
MsaG035081	AT2G26700.1	48.649	185	83	5	8	186	14	192	7.63e-46	158
MsaG035081	AT5G40030.1	48.148	135	67	1	52	186	86	217	2.85e-39	140
MsaG035081	AT3G52890.2	51.852	135	61	2	52	186	511	641	4.27e-39	142
MsaG035081	AT3G52890.4	51.852	135	61	2	52	186	511	641	4.27e-39	142
MsaG035081	AT3G52890.1	51.852	135	61	2	52	186	511	641	4.27e-39	142
MsaG035081	AT3G52890.3	51.852	135	61	2	52	186	511	641	4.27e-39	142
MsaG035081	AT5G55910.2	48.889	135	65	2	52	186	82	212	7.65e-39	139
MsaG035081	AT5G55910.1	48.889	135	65	2	52	186	82	212	7.65e-39	139
MsaG035081	AT4G26610.1	44.186	172	78	4	15	186	73	226	8.43e-39	139
MsaG035081	AT2G36350.1	52.419	124	55	2	52	175	532	651	2.62e-38	139
MsaG035081	AT5G03640.2	41.954	174	95	3	14	186	476	644	1.21e-37	137
MsaG035081	AT5G03640.1	41.954	174	95	3	14	186	476	644	1.21e-37	137
MsaG035081	AT5G47750.1	51.200	125	57	2	52	176	164	284	3.06e-37	135
MsaG035081	AT3G12690.1	47.183	142	71	2	45	186	151	288	5.90e-37	134
MsaG035081	AT3G12690.3	47.183	142	71	2	45	186	151	288	5.90e-37	134
MsaG035081	AT3G12690.2	47.183	142	71	2	45	186	151	288	5.90e-37	134
MsaG035081	AT1G16440.1	47.407	135	69	2	52	186	86	218	2.38e-35	129
MsaG035081	AT2G44830.2	47.407	135	67	2	52	186	336	466	2.98e-35	130
MsaG035081	AT2G44830.3	47.407	135	67	2	52	186	336	466	2.98e-35	130
MsaG035081	AT2G44830.1	47.407	135	67	2	52	186	336	466	2.98e-35	130
MsaG035081	AT1G79250.1	47.407	135	67	2	52	186	119	249	4.22e-35	129
MsaG035081	AT1G79250.3	47.407	135	67	2	52	186	119	249	4.22e-35	129
MsaG035081	AT1G79250.2	47.407	135	67	2	52	186	119	249	4.22e-35	129
MsaG035081	AT1G79250.4	47.407	135	67	2	52	186	119	249	4.22e-35	129
MsaG035081	AT1G53700.1	46.269	134	69	3	54	186	66	197	1.19e-34	127
MsaG035081	AT3G27580.2	46.377	138	70	2	50	186	151	285	4.40e-34	127
MsaG035081	AT3G27580.1	46.377	138	70	2	50	186	151	285	4.40e-34	127
MsaG035081	AT3G14370.1	41.791	134	74	2	54	186	61	191	5.71e-31	117
MsaG035081	AT5G58140.4	36.567	134	81	2	53	186	551	680	1.48e-24	100
MsaG035081	AT5G58140.6	36.567	134	81	2	53	186	551	680	1.65e-24	100
MsaG035081	AT5G58140.7	36.567	134	81	2	53	186	551	680	1.65e-24	100
MsaG035081	AT5G58140.5	36.567	134	81	2	53	186	551	680	1.65e-24	100
MsaG035081	AT5G58140.2	36.567	134	81	2	53	186	551	680	1.65e-24	100
MsaG035081	AT5G58140.1	36.567	134	81	2	53	186	551	680	1.65e-24	100
MsaG035081	AT5G58140.3	36.567	134	81	2	53	186	551	680	1.95e-24	100
MsaG035081	AT3G44610.1	44.037	109	54	1	85	186	74	182	1.79e-23	96.7
MsaG035081	AT3G45780.2	33.333	147	94	2	40	186	624	766	1.30e-20	89.0
MsaG035081	AT3G45780.1	33.333	147	94	2	40	186	624	766	1.30e-20	89.0
MsaG035081	AT3G08720.1	33.333	96	61	1	73	168	133	225	3.21e-12	64.7
MsaG035081	AT3G08720.3	33.333	96	61	1	73	168	133	225	3.21e-12	64.7
MsaG035081	AT3G08720.4	33.333	96	61	1	73	168	133	225	3.21e-12	64.7
MsaG035081	AT3G08720.2	33.333	96	61	1	73	168	133	225	3.21e-12	64.7
MsaG035081	AT3G08730.1	29.245	106	72	1	63	168	117	219	6.55e-11	60.8
MsaG035081	AT3G08730.2	29.245	106	72	1	63	168	117	219	6.55e-11	60.8

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATTTCTCTTGCATTGTTA+TGG	0.264166	7:+6942783	MsaT035081.1:CDS
GAAATCACATAGAGCTGAAA+TGG	0.348283	7:+6942693	MsaT035081.1:CDS
CGTATCGGCGCCGGAGATAT+AGG	0.415019	7:+6942577	MsaT035081.1:CDS
TGTGGTACCATGTTAGAAAC+TGG	0.426673	7:+6942316	None:intergenic
GCATAGAGTGTAGGAAGAAA+AGG	0.432484	7:-6942745	None:intergenic
GTGAGGGTGTTTGTGGCGTA+TGG	0.435424	7:-6942833	None:intergenic
CGGAGGAGCGGTGAGGTTTG+AGG	0.441661	7:-6942474	None:intergenic
GGAGGAGCGGTGAGGTTTGA+GGG	0.445021	7:-6942473	None:intergenic
CTTCGTTTCTGAGGCGGCAG+AGG	0.467146	7:-6942609	None:intergenic
CTATATCTCCGGCGCCGATA+CGG	0.467445	7:-6942576	None:intergenic
CGACCTTCTTCCGCCTTCAC+CGG	0.471504	7:+6942441	MsaT035081.1:CDS
CGGCGCCGATACGGCGGAGG+AGG	0.472990	7:-6942567	None:intergenic
CACTTGTTTGTATGCGATGA+AGG	0.483898	7:+6942639	MsaT035081.1:CDS
GACGGTTGTGAGGGTGTTTG+TGG	0.483934	7:-6942840	None:intergenic
CTCCTCCGCCGTATCGGCGC+CGG	0.505028	7:+6942568	MsaT035081.1:CDS
ATTTACCTAGCGGAAGTAAG+AGG	0.510467	7:-6942865	None:intergenic
GGTTTGAGGGAGAGACTCTC+CGG	0.513127	7:-6942460	None:intergenic
TTTCACCTCCTCCGCCGTAT+CGG	0.514538	7:+6942562	MsaT035081.1:CDS
TCTCCGGTGAAGGCGGAAGA+AGG	0.524425	7:-6942444	None:intergenic
CTCCGGCGCCGATACGGCGG+AGG	0.524516	7:-6942570	None:intergenic
GTAAACAGTGCCTATATCTC+CGG	0.533869	7:-6942587	None:intergenic
TGTCATCTTCTTCGTTTCTG+AGG	0.535049	7:-6942618	None:intergenic
TAAGAGGAAGACGGTTGTGA+GGG	0.539144	7:-6942849	None:intergenic
TTTGTATGCGATGAAGGTTG+TGG	0.541682	7:+6942645	MsaT035081.1:CDS
GTAAGAGGAAGACGGTTGTG+AGG	0.548424	7:-6942850	None:intergenic
AGGGAGAGACTCTCCGGTGA+AGG	0.570898	7:-6942454	None:intergenic
ATGTGAGAGCGGACTTGCGA+CGG	0.580197	7:-6942531	None:intergenic
CATCTTCTTCGTTTCTGAGG+CGG	0.581374	7:-6942615	None:intergenic
TCAAACTCAGCATAGAGTGT+AGG	0.584379	7:-6942754	None:intergenic
TATCTCCGGCGCCGATACGG+CGG	0.593136	7:-6942573	None:intergenic
GAGAGACTCTCCGGTGAAGG+CGG	0.595659	7:-6942451	None:intergenic
GAAGGTAGCGGAACGGATGG+CGG	0.596507	7:-6942509	None:intergenic
GATGGCGGAGTAAGCGAAGT+CGG	0.597879	7:-6942494	None:intergenic
ACGGAAGGTAGCGGAACGGA+TGG	0.603297	7:-6942512	None:intergenic
GGACTTGCGACGGAAGGTAG+CGG	0.609884	7:-6942521	None:intergenic
TATGGAATGCAAGTCACCAC+CGG	0.613302	7:-6942815	None:intergenic
GATGAAGGTTGTGGATAAAG+AGG	0.646934	7:+6942654	MsaT035081.1:CDS
GAAGAAGGTCGAATGAAAGA+CGG	0.658019	7:-6942429	None:intergenic
AGTAAGCGAAGTCGGAGGAG+CGG	0.659359	7:-6942486	None:intergenic
GGCGGAGGAGGTGAAAGTCA+CGG	0.667380	7:-6942555	None:intergenic
GTCTTCCTCTTACTTCCGCT+AGG	0.667703	7:+6942860	MsaT035081.1:CDS
ACGTGTACCAGTTTCTAACA+TGG	0.671516	7:-6942323	None:intergenic
GAGAGCGGACTTGCGACGGA+AGG	0.672834	7:-6942527	None:intergenic
TAGCGGAAGTAAGAGGAAGA+CGG	0.675534	7:-6942858	None:intergenic
AAAGTCACGGAATGTGAGAG+CGG	0.682400	7:-6942542	None:intergenic
GCGAAGTCGGAGGAGCGGTG+AGG	0.701596	7:-6942481	None:intergenic
TGCGACGGAAGGTAGCGGAA+CGG	0.710050	7:-6942516	None:intergenic
GGCGGAGTAAGCGAAGTCGG+AGG	0.716616	7:-6942491	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	6942325	6942885	6942325	ID=MsaG035081
Chr7	mRNA	6942325	6942885	6942325	ID=MsaT035081.1;Parent=MsaG035081
Chr7	exon	6942325	6942885	6942325	ID=MsaT035081.1.exon1;Parent=MsaT035081.1
Chr7	CDS	6942325	6942885	6942325	ID=cds.MsaT035081.1;Parent=MsaT035081.1

Gene Sequence

>MsaT035081.1

ATGTTAGAAACTGGTACACGTGATTCTGAGTTGAGTTTAGAAACTGTGAATTCAACACAACGAAGCTCAATGAGCAGTGAAAGCATTTGCAGCACTAGTTTCAGCCGTCTTTCATTCGACCTTCTTCCGCCTTCACCGGAGAGTCTCTCCCTCAAACCTCACCGCTCCTCCGACTTCGCTTACTCCGCCATCCGTTCCGCTACCTTCCGTCGCAAGTCCGCTCTCACATTCCGTGACTTTCACCTCCTCCGCCGTATCGGCGCCGGAGATATAGGCACTGTTTACCTCTGCCGCCTCAGAAACGAAGAAGATGACACTTGTTTGTATGCGATGAAGGTTGTGGATAAAGAGGTTGTTGCATTGAAGAAGAAATCACATAGAGCTGAAATGGAGAGAAAGATTCTGAAAATGCTTGATCATCCTTTTCTTCCTACACTCTATGCTGAGTTTGAAGCTTCTCATTTCTCTTGCATTGTTATGGAATTTTGTTCCGGTGGTGACTTGCATTCCATACGCCACAAACACCCTCACAACCGTCTTCCTCTTACTTCCGCTAGGTAA

Protein sequence

>MsaT035081.1

MLETGTRDSELSLETVNSTQRSSMSSESICSTSFSRLSFDLLPPSPESLSLKPHRSSDFAYSAIRSATFRRKSALTFRDFHLLRRIGAGDIGTVYLCRLRNEEDDTCLYAMKVVDKEVVALKKKSHRAEMERKILKMLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHSIRHKHPHNRLPLTSAR*