AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035215


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035215 MtrunA17_Chr7g0214501 86.932 352 4 1 86 437 1 310 0.0 619
MsaG035215 MtrunA17_Chr1g0213311 61.598 388 132 6 1 377 1 382 1.12e-167 477
MsaG035215 MtrunA17_Chr7g0214491 97.647 85 2 0 1 85 1 85 5.20e-57 183
MsaG035215 MtrunA17_Chr3g0079841 38.079 302 124 6 22 323 26 264 2.43e-54 182
MsaG035215 MtrunA17_Chr3g0143581 51.852 135 61 3 25 156 20 153 2.64e-40 142
MsaG035215 MtrunA17_Chr3g0143571 45.098 153 78 2 190 337 23 174 9.26e-37 132
MsaG035215 MtrunA17_Chr5g0435401 23.711 291 159 7 67 353 70 301 2.59e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035215 AT1G04880.1 55.080 374 139 6 1 356 1 363 3.74e-121 360
MsaG035215 AT1G76110.1 42.812 313 156 3 25 327 27 326 1.08e-81 255
MsaG035215 AT3G13350.1 47.492 299 125 8 30 323 34 305 3.84e-79 248
MsaG035215 AT1G55650.1 39.867 301 140 6 32 331 30 290 2.76e-64 210

Find 92 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTACAGTGTCTTCTTC+TGG 0.218418 7:-9088460 MsaT035215.1:intron
GGAGAAGCTTCATGCTTCTA+TGG 0.249345 7:-9090827 MsaT035215.1:CDS
GGAAGAAGTCAGAAATAAAA+AGG 0.265856 7:-9088264 MsaT035215.1:CDS
TGTTTCAGGGTTCCCATTAT+TGG 0.277531 7:-9090612 MsaT035215.1:intron
CGGTGGACCCCTGCATCTTC+TGG 0.281007 7:-9089420 MsaT035215.1:intron
TCTTCTGGATCTTCACTTGT+TGG 0.289352 7:-9088445 MsaT035215.1:CDS
GCCACTTCAACAACGGTTTC+CGG 0.299855 7:-9082179 MsaT035215.1:CDS
ATAGGAGCTACCACTTTCAT+AGG 0.334690 7:+9090925 None:intergenic
TCACTTGTTGGAGTTATTGA+TGG 0.342127 7:-9088433 MsaT035215.1:CDS
TAACCACTTCTGTTAGGTTT+CGG 0.349489 7:+9088226 None:intergenic
TAATATCCTTGTCCATGGTT+TGG 0.351606 7:+9088158 None:intergenic
AAGTCGACTGGATGTGTTCC+TGG 0.356132 7:+9089241 None:intergenic
TCCTCCTCCGACGGCGGCGT+TGG 0.371177 7:+9088284 None:intergenic
CAATATTGCATCTGCTGCAT+TGG 0.389249 7:-9088311 MsaT035215.1:CDS
GAAGCATTTGTTGCTGTTGA+TGG 0.394767 7:+9089513 None:intergenic
ACTCCCGAGGAGAGTAGTTC+TGG 0.398649 7:-9082103 MsaT035215.1:CDS
AGATGCAGGGGTCCACCGTT+TGG 0.409841 7:+9089425 None:intergenic
AAAATTGTCTACACTAAAGT+AGG 0.410247 7:+9081882 None:intergenic
AAATTGTAACCACTTCTGTT+AGG 0.413038 7:+9088220 None:intergenic
GTAGAATGATTGGCGAACTT+TGG 0.413562 7:-9088135 MsaT035215.1:CDS
TTATCAAGAAAAGGCTGTTA+AGG 0.420019 7:-9082278 MsaT035215.1:CDS
AAAGTAGGGATAACATGTTT+TGG 0.420130 7:+9081897 None:intergenic
AGAAAGGTACCAAGCAGAAA+TGG 0.423266 7:-9082251 MsaT035215.1:CDS
ATCAACATAGATGCTTCTTC+CGG 0.439105 7:-9082055 MsaT035215.1:CDS
CTGTTAGGTTTCGGATGTGC+AGG 0.443824 7:+9088235 None:intergenic
CAAGCTCTTTATGCTTACAT+TGG 0.448632 7:-9090848 MsaT035215.1:CDS
CCAGTCGACTTTCAGAAGTC+AGG 0.453438 7:-9089229 MsaT035215.1:CDS
CATTAAGGTTTATCAAGAAA+AGG 0.456846 7:-9082287 MsaT035215.1:intron
CAATGTAAGCATAAAGAGCT+TGG 0.458599 7:+9090849 None:intergenic
ATTGATGGAAAGTTTGAAAG+TGG 0.459678 7:-9088418 MsaT035215.1:CDS
AAGGATATTAGTAGAATGAT+TGG 0.462358 7:-9088145 MsaT035215.1:CDS
TCCGGAAGGAGATTCAGCTT+TGG 0.463701 7:-9082161 MsaT035215.1:CDS
GGAAGGAGATTCAGCTTTGG+TGG 0.464831 7:-9082158 MsaT035215.1:CDS
AGAATCCACTCTTGCTCCAC+CGG 0.466317 7:+9082036 None:intergenic
AATATTGCATCTGCTGCATT+GGG 0.466960 7:-9088310 MsaT035215.1:CDS
TGATTCAGACAAGGCAACTA+AGG 0.470199 7:-9082002 MsaT035215.1:CDS
TCCAACGCCGCCGTCGGAGG+AGG 0.470219 7:-9088285 MsaT035215.1:CDS
AGCTTATGAAAGAGAGAAGA+TGG 0.479121 7:-9089563 MsaT035215.1:CDS
ACCAAAGCTGAATCTCCTTC+CGG 0.486239 7:+9082160 None:intergenic
TATCTAGTTTCAGTTTCAGT+AGG 0.493509 7:-9088394 MsaT035215.1:CDS
AATGTAAGCATAAAGAGCTT+GGG 0.499008 7:+9090850 None:intergenic
TTGAAGTGGCCCCGCGCTGT+CGG 0.500198 7:+9082192 None:intergenic
TCCGGAAACCGTTGTTGAAG+TGG 0.505882 7:+9082178 None:intergenic
ACTGGATGTGTTCCTGGAGA+AGG 0.512094 7:+9089247 None:intergenic
TTCAGGGTTCCCATTATTGG+AGG 0.517053 7:-9090609 MsaT035215.1:intron
GAGAAGCTTCATGCTTCTAT+GGG 0.518689 7:-9090826 MsaT035215.1:CDS
GCGCGGGGCCACTTCAACAA+CGG 0.523750 7:-9082186 MsaT035215.1:CDS
TGGGCGTCCAACGCCGCCGT+CGG 0.523765 7:-9088291 MsaT035215.1:CDS
TTCATTGAAGTGACTTCTCG+TGG 0.526953 7:-9090561 MsaT035215.1:CDS
ATGCACAAACCAGAAGATGC+AGG 0.528390 7:+9089411 None:intergenic
CAATGATTTCCCTATGAAAG+TGG 0.531885 7:-9090935 MsaT035215.1:CDS
TTCACCAGAACTACTCTCCT+CGG 0.534121 7:+9082099 None:intergenic
GATAATCCTCCATTTCTGCT+TGG 0.539679 7:+9082242 None:intergenic
TCTACTAATATCCTTGTCCA+TGG 0.543804 7:+9088153 None:intergenic
CAGTCGACTTTCAGAAGTCA+GGG 0.544869 7:-9089228 MsaT035215.1:CDS
CCTGACTTCTGAAAGTCGAC+TGG 0.548629 7:+9089229 None:intergenic
AGCATTGTAACCGACAGCGC+GGG 0.549400 7:-9082202 MsaT035215.1:CDS
TGCACAAACCAGAAGATGCA+GGG 0.551653 7:+9089412 None:intergenic
TCACCAGAACTACTCTCCTC+GGG 0.553627 7:+9082100 None:intergenic
GCGTCCAACGCCGCCGTCGG+AGG 0.562184 7:-9088288 MsaT035215.1:CDS
TTGAAGTGACTTCTCGTGGA+GGG 0.562395 7:-9090557 MsaT035215.1:CDS
AACATAGATGCTTCTTCCGG+TGG 0.562713 7:-9082052 MsaT035215.1:CDS
GAAGTTCTTAGCCATTGTGA+GGG 0.564422 7:-9081968 MsaT035215.1:CDS
TCAGGATATGAATGAGACAT+AGG 0.570116 7:+9090907 None:intergenic
TTCTTCCGGTGGAGCAAGAG+TGG 0.579438 7:-9082041 MsaT035215.1:CDS
TGAAGTTCTTAGCCATTGTG+AGG 0.580768 7:-9081969 MsaT035215.1:CDS
CTGACTTCTTCCTCCTCCGA+CGG 0.584477 7:+9088275 None:intergenic
TATTGAAGCTGGAACAGACA+TGG 0.585157 7:+9089285 None:intergenic
TGTGAGGGAGAAGTGAATGC+TGG 0.586039 7:-9081953 MsaT035215.1:CDS
AGGCTGTTAAGGATAAAGAA+AGG 0.597432 7:-9082267 MsaT035215.1:CDS
AAATTGTCTACACTAAAGTA+GGG 0.600990 7:+9081883 None:intergenic
TCAGACAAGGCAACTAAGGA+GGG 0.601091 7:-9081998 MsaT035215.1:CDS
TGCAAAATATGATGATGTTG+TGG 0.605066 7:-9090878 MsaT035215.1:CDS
ACTTCTTCCTCCTCCGACGG+CGG 0.605098 7:+9088278 None:intergenic
CATCCGAAACCTAACAGAAG+TGG 0.610656 7:-9088229 MsaT035215.1:CDS
TTCAGACAAGGCAACTAAGG+AGG 0.611052 7:-9081999 MsaT035215.1:CDS
AAAGCTACTAAACCAAACCA+TGG 0.615278 7:-9088170 MsaT035215.1:CDS
AATGCTTCTGAATTGCCTCA+AGG 0.618648 7:-9089202 MsaT035215.1:intron
CTTCAACAACGGTTTCCGGA+AGG 0.622063 7:-9082175 MsaT035215.1:CDS
TAGGAGCTACCACTTTCATA+GGG 0.623246 7:+9090926 None:intergenic
AAGGTACCAAGCAGAAATGG+AGG 0.630994 7:-9082248 MsaT035215.1:CDS
CATTCACTTCTCCCTCACAA+TGG 0.653715 7:+9081957 None:intergenic
TTCTCGTGGAGGGATTGAGA+AGG 0.661472 7:-9090547 MsaT035215.1:intron
GCACAAACCAGAAGATGCAG+GGG 0.670000 7:+9089413 None:intergenic
ACTAAACCAAACCATGGACA+AGG 0.670033 7:-9088164 MsaT035215.1:CDS
GTAGGTTCAGAGACACTCAA+AGG 0.670192 7:-9088376 MsaT035215.1:CDS
GCATTGTAACCGACAGCGCG+GGG 0.671610 7:-9082201 MsaT035215.1:CDS
ATTGAAGTGACTTCTCGTGG+AGG 0.683803 7:-9090558 MsaT035215.1:CDS
AGTCGACTTTCAGAAGTCAG+GGG 0.692181 7:-9089227 MsaT035215.1:CDS
GAGCATTGTAACCGACAGCG+CGG 0.694172 7:-9082203 MsaT035215.1:CDS
GAGGGAGAAGTGAATGCTGG+AGG 0.708907 7:-9081950 MsaT035215.1:CDS
CGATTCGTGTCAGACTCCCG+AGG 0.736217 7:-9082116 MsaT035215.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 9081895 9090986 9081895 ID=MsaG035215
Chr7 mRNA 9081895 9090986 9081895 ID=MsaT035215.1;Parent=MsaG035215
Chr7 exon 9081895 9082302 9081895 ID=MsaT035215.1.exon6;Parent=MsaT035215.1
Chr7 CDS 9081895 9082302 9081895 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Chr7 exon 9088108 9088472 9088108 ID=MsaT035215.1.exon5;Parent=MsaT035215.1
Chr7 CDS 9088108 9088472 9088108 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Chr7 exon 9089203 9089328 9089203 ID=MsaT035215.1.exon4;Parent=MsaT035215.1
Chr7 CDS 9089203 9089328 9089203 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Chr7 exon 9089421 9089583 9089421 ID=MsaT035215.1.exon3;Parent=MsaT035215.1
Chr7 CDS 9089421 9089583 9089421 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Chr7 exon 9090548 9090626 9090548 ID=MsaT035215.1.exon2;Parent=MsaT035215.1
Chr7 CDS 9090548 9090626 9090548 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Chr7 exon 9090814 9090986 9090814 ID=MsaT035215.1.exon1;Parent=MsaT035215.1
Chr7 CDS 9090814 9090986 9090814 ID=cds.MsaT035215.1;Parent=MsaT035215.1
Gene Sequence

>MsaT035215.1

ATGGCATCAACATCATGTTTTTTCAACAACAATGATTTCCCTATGAAAGTGGTAGCTCCTATGTCTCATTCATATCCTGAACCTCTTGCAAAATATGATGATGTTGTGGCAAATCCCAAGCTCTTTATGCTTACATTGGAGAAGCTTCATGCTTCTATGGGTACTAAGTTCATGGTTCCCATTATTGGAGGAAAAGAACTAGATTTGTGTCGACTCTTCATTGAAGTGACTTCTCGTGGAGGGATTGAGAAGCTTATGAAAGAGAGAAGATGGAAAGAAGTAACTGCAGCTTTCAGTTTTCCATCAACAGCAACAAATGCTTCCTTTGTGCTCAGAAAATACTATTCTTCATTGCTTTATCACTATGAGCAAATCTACTATTTTAGATCCAAACGGTGGACCCCTGCATCTTCTGATGCTTTGCAGAATCAATCAACCATGTCTGTTCCAGCTTCAATAACGCAATTTTTGCAGCCTTCTCCAGGAACACATCCAGTCGACTTTCAGAAGTCAGGGGTTAATGCTTCTGAATTGCCTCAAGTGTCTTCTTCTGGATCTTCACTTGTTGGAGTTATTGATGGAAAGTTTGAAAGTGGATATCTAGTTTCAGTTTCAGTAGGTTCAGAGACACTCAAAGGTGTACTATATGAGTCTCCACAAAGCATTAAAATTAATAATAACAATATTGCATCTGCTGCATTGGGCGTCCAACGCCGCCGTCGGAGGAGGAAGAAGTCAGAAATAAAAAGGAGAGATCCTGCACATCCGAAACCTAACAGAAGTGGTTACAATTTCTTCTTTGCTGAACAACATGCAAGACTAAAGCTACTAAACCAAACCATGGACAAGGATATTAGTAGAATGATTGGCGAACTTTGGAACAACTTAAAAGAGTCTGAGAAAACAGTTTATCAAGAAAAGGCTGTTAAGGATAAAGAAAGGTACCAAGCAGAAATGGAGGATTATCGCGATAAAATGAAGACGAGCATTGTAACCGACAGCGCGGGGCCACTTCAACAACGGTTTCCGGAAGGAGATTCAGCTTTGGTGGACGTTGACATAAAAATGCACGATTCGTGTCAGACTCCCGAGGAGAGTAGTTCTGGTGAAAGTGACTATGTTGCAGATGATATCAACATAGATGCTTCTTCCGGTGGAGCAAGAGTGGATTCTGAAGCTTTTGTTGATTCAGACAAGGCAACTAAGGAGGGAGTTATTGAAGTTCTTAGCCATTGTGAGGGAGAAGTGAATGCTGGAGGAATTCAACAGACACAAAAGATGAATGAGAGCCAAAACATGTTATCCCTACTTTAG

Protein sequence

>MsaT035215.1

MASTSCFFNNNDFPMKVVAPMSHSYPEPLAKYDDVVANPKLFMLTLEKLHASMGTKFMVPIIGGKELDLCRLFIEVTSRGGIEKLMKERRWKEVTAAFSFPSTATNASFVLRKYYSSLLYHYEQIYYFRSKRWTPASSDALQNQSTMSVPASITQFLQPSPGTHPVDFQKSGVNASELPQVSSSGSSLVGVIDGKFESGYLVSVSVGSETLKGVLYESPQSIKINNNNIASAALGVQRRRRRRKKSEIKRRDPAHPKPNRSGYNFFFAEQHARLKLLNQTMDKDISRMIGELWNNLKESEKTVYQEKAVKDKERYQAEMEDYRDKMKTSIVTDSAGPLQQRFPEGDSALVDVDIKMHDSCQTPEESSSGESDYVADDINIDASSGGARVDSEAFVDSDKATKEGVIEVLSHCEGEVNAGGIQQTQKMNESQNMLSLL*