AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035314 MtrunA17_Chr2g0281511 86.885 122 16 0 1 122 1 122 1.95e-75 219
MsaG035314 MtrunA17_Chr2g0309121 81.967 122 21 1 1 122 1 121 8.82e-69 202
MsaG035314 MtrunA17_Chr3g0081891 88.750 80 9 0 1 80 1 80 1.07e-46 145
MsaG035314 MtrunA17_Chr7g0276661 32.847 137 72 4 2 120 33 167 1.88e-17 73.6
MsaG035314 MtrunA17_Chr0c01g0489371 41.758 91 51 1 22 110 224 314 2.23e-16 73.2
MsaG035314 MtrunA17_Chr5g0430431 36.667 90 47 1 37 116 165 254 1.17e-15 70.5
MsaG035314 MtrunA17_Chr8g0356961 39.560 91 53 1 22 110 75 165 1.31e-15 68.9
MsaG035314 MtrunA17_Chr5g0434641 37.363 91 55 1 22 110 208 298 9.72e-15 68.6
MsaG035314 MtrunA17_Chr8g0372431 26.804 97 71 0 24 120 6 102 1.96e-14 64.3
MsaG035314 MtrunA17_Chr8g0356951 38.462 91 52 3 24 110 217 307 7.10e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTTACTACTTCTATTT+TGG 0.135781 7:+10740441 MsaT035314.1:CDS
GAAGTCAATGGTACTGAATT+AGG 0.159497 7:+10740382 MsaT035314.1:CDS
AAAATGTCGATGTTCATTAT+TGG 0.262235 7:+10740413 MsaT035314.1:CDS
TACCGGTTTCTTCATCAAAA+AGG 0.272684 7:-10740635 None:intergenic
TGCATGCTACTGTCGTTAAC+TGG 0.310301 7:+10740802 MsaT035314.1:CDS
TGTTCATTATTGGAAGTATC+AGG 0.316986 7:+10740423 MsaT035314.1:CDS
ATTACAATCATAAGATTTAC+CGG 0.343726 7:-10740652 None:intergenic
ACAGTAGCATGCATCCTATT+AGG 0.354672 7:-10740792 None:intergenic
CGGTCAATAATCCTCCTAAT+AGG 0.355854 7:+10740778 MsaT035314.1:CDS
TTTGGATGGAAACAATGTTT+TGG 0.356448 7:+10740459 MsaT035314.1:CDS
CCTCGACATGTTGCTGAGAA+TGG 0.367959 7:+10740585 MsaT035314.1:CDS
GTACGACTTTGTTCTTGAAA+AGG 0.412286 7:+10740725 MsaT035314.1:CDS
AAGTCAATGGTACTGAATTA+GGG 0.437113 7:+10740383 MsaT035314.1:CDS
TACGACTTTGTTCTTGAAAA+GGG 0.450393 7:+10740726 MsaT035314.1:CDS
GCATGCATCAGAAATACATA+GGG 0.517782 7:+10740694 MsaT035314.1:CDS
AGCATGCATCAGAAATACAT+AGG 0.534012 7:+10740693 MsaT035314.1:CDS
GCATGCTACTGTCGTTAACT+GGG 0.542979 7:+10740803 MsaT035314.1:CDS
GAGATTGAAGTTGAAGTCAA+TGG 0.550805 7:+10740370 MsaT035314.1:CDS
CCATTCTCAGCAACATGTCG+AGG 0.623541 7:-10740585 None:intergenic
GTAGCATGCATCCTATTAGG+AGG 0.626434 7:-10740789 None:intergenic
ATGCATCAGAAATACATAGG+GGG 0.708581 7:+10740696 MsaT035314.1:CDS
CATGCATCAGAAATACATAG+GGG 0.718850 7:+10740695 MsaT035314.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 10740364 10740830 10740364 ID=MsaG035314
Chr7 mRNA 10740364 10740830 10740364 ID=MsaT035314.1;Parent=MsaG035314
Chr7 exon 10740364 10740480 10740364 ID=MsaT035314.1.exon1;Parent=MsaT035314.1
Chr7 CDS 10740364 10740480 10740364 ID=cds.MsaT035314.1;Parent=MsaT035314.1
Chr7 exon 10740579 10740830 10740579 ID=MsaT035314.1.exon2;Parent=MsaT035314.1
Chr7 CDS 10740579 10740830 10740579 ID=cds.MsaT035314.1;Parent=MsaT035314.1
Gene Sequence

>MsaT035314.1

ATGAATGAGATTGAAGTTGAAGTCAATGGTACTGAATTAGGGAATGTTCAAAATGTCGATGTTCATTATTGGAAGTATCAGGTTACTACTTCTATTTTGGATGGAAACAATGTTTTGCATTTTCCTCGACATGTTGCTGAGAATGGTTTGTATATCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACCGGTAAATCTTATGATTGTAATGTTCACACTGCGTCAAAAAGCATGCATCAGAAATACATAGGGGGAGGATGGTACGACTTTGTTCTTGAAAAGGGCCTAAAAGTTGATGACATTATTTTGTTCACGGTCAATAATCCTCCTAATAGGATGCATGCTACTGTCGTTAACTGGGACTGA
Protein sequence

>MsaT035314.1

MNEIEVEVNGTELGNVQNVDVHYWKYQVTTSILDGNNVLHFPRHVAENGLYINQSKILLFDEETGKSYDCNVHTASKSMHQKYIGGGWYDFVLEKGLKVDDIILFTVNNPPNRMHATVVNWD*