AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035599


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035599 MtrunA17_Chr7g0218891 83.150 273 46 0 1 273 1 273 2.83e-157 438
MsaG035599 MtrunA17_Chr4g0075861 33.761 234 146 4 26 259 20 244 2.02e-32 122
MsaG035599 MtrunA17_Chr4g0075861 37.086 151 89 2 112 262 20 164 7.73e-25 102
MsaG035599 MtrunA17_Chr4g0075861 35.088 171 102 5 5 173 81 244 2.91e-18 83.6
MsaG035599 MtrunA17_Chr4g0075971 33.333 234 147 4 26 259 20 244 1.02e-29 117
MsaG035599 MtrunA17_Chr4g0075971 35.762 151 91 2 112 262 20 164 1.60e-22 96.7
MsaG035599 MtrunA17_Chr4g0075971 35.673 171 101 5 5 173 81 244 2.20e-17 81.6
MsaG035599 MtrunA17_Chr3g0077891 39.053 169 95 3 99 261 8 174 1.96e-27 104
MsaG035599 MtrunA17_Chr3g0077891 37.349 166 96 3 9 168 4 167 5.28e-24 95.5
MsaG035599 MtrunA17_Chr1g0206771 43.066 137 71 2 118 254 33 162 1.28e-25 99.8
MsaG035599 MtrunA17_Chr1g0206771 37.821 156 86 4 14 168 17 162 4.57e-21 87.8
MsaG035599 MtrunA17_Chr3g0077941 37.500 168 98 3 99 261 8 173 6.49e-24 95.1
MsaG035599 MtrunA17_Chr3g0077941 35.882 170 102 5 9 173 4 171 8.03e-20 84.3
MsaG035599 MtrunA17_Chr3g0077881 37.952 166 94 4 99 258 8 170 1.51e-23 94.4
MsaG035599 MtrunA17_Chr3g0077881 34.756 164 99 3 11 168 6 167 3.84e-19 82.4
MsaG035599 MtrunA17_Chr3g0077881 46.250 80 42 1 186 265 9 87 4.85e-14 68.6
MsaG035599 MtrunA17_Chr8g0364231 40.580 138 78 3 115 250 56 191 5.11e-23 98.6
MsaG035599 MtrunA17_Chr8g0364231 38.406 138 81 3 29 164 56 191 3.74e-19 87.4
MsaG035599 MtrunA17_Chr8g0364231 39.785 93 55 1 171 262 25 117 8.86e-14 71.2
MsaG035599 MtrunA17_Chr1g0205821 34.337 166 103 3 99 262 13 174 7.66e-23 92.4
MsaG035599 MtrunA17_Chr1g0205821 30.864 162 106 3 11 170 11 168 2.61e-17 77.8
MsaG035599 MtrunA17_Chr3g0077861 37.748 151 90 3 105 252 7 156 7.94e-23 92.0
MsaG035599 MtrunA17_Chr3g0077861 38.129 139 78 3 32 166 22 156 1.58e-19 83.2
MsaG035599 MtrunA17_Chr3g0077911 36.774 155 94 3 101 252 3 156 2.20e-22 90.9
MsaG035599 MtrunA17_Chr3g0077911 38.849 139 77 3 32 166 22 156 2.12e-21 88.2
MsaG035599 MtrunA17_Chr1g0205831 34.356 163 86 4 102 262 5 148 4.22e-21 87.8
MsaG035599 MtrunA17_Chr1g0205831 34.568 162 89 3 16 177 5 149 6.87e-21 87.4
MsaG035599 MtrunA17_Chr5g0415031 35.915 142 81 3 115 251 57 193 4.19e-19 87.0
MsaG035599 MtrunA17_Chr5g0415031 34.752 141 84 3 29 165 57 193 5.24e-16 77.8
MsaG035599 MtrunA17_Chr5g0415031 45.455 66 36 0 201 266 57 122 1.39e-11 64.7
MsaG035599 MtrunA17_Chr6g0452001 33.544 158 99 3 95 250 59 212 3.43e-18 84.3
MsaG035599 MtrunA17_Chr6g0452001 32.026 153 98 3 14 164 64 212 7.32e-15 74.7
MsaG035599 MtrunA17_Chr6g0452021 33.553 152 97 2 99 250 43 190 4.80e-18 83.6
MsaG035599 MtrunA17_Chr6g0452021 31.126 151 100 2 14 164 44 190 3.61e-15 75.1
MsaG035599 MtrunA17_Chr1g0205811 45.000 80 42 1 183 262 12 89 5.05e-14 67.4
MsaG035599 MtrunA17_Chr1g0205811 40.206 97 52 2 100 192 15 109 1.29e-12 63.5
MsaG035599 MtrunA17_Chr1g0205801 46.753 77 39 1 186 262 13 87 1.36e-13 65.9
MsaG035599 MtrunA17_Chr1g0205801 44.156 77 41 1 100 176 13 87 1.31e-12 63.2
MsaG035599 MtrunA17_Chr1g0205801 45.679 81 41 2 11 90 10 88 1.62e-12 62.8
MsaG035599 MtrunA17_Chr1g0206781 30.827 133 64 2 91 223 3 107 4.49e-13 64.7
MsaG035599 MtrunA17_Chr1g0206781 38.372 86 51 1 177 262 3 86 1.54e-12 63.2
MsaG035599 MtrunA17_Chr1g0206781 31.818 132 62 4 5 136 3 106 3.72e-12 62.0
MsaG035599 MtrunA17_Chr1g0205861 46.875 64 32 1 186 249 17 78 5.61e-11 58.9
MsaG035599 MtrunA17_Chr1g0205861 38.202 89 51 2 95 183 12 96 8.90e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035599 AT5G15020.2 37.975 158 94 3 93 250 46 199 3.25e-21 93.6
MsaG035599 AT5G15020.2 35.948 153 94 3 14 166 53 201 2.77e-17 82.0
MsaG035599 AT5G15020.1 37.975 158 94 3 93 250 46 199 3.32e-21 93.6
MsaG035599 AT5G15020.1 35.948 153 94 3 14 166 53 201 2.94e-17 82.0
MsaG035599 AT1G24190.2 37.037 162 96 5 91 250 6 163 5.82e-21 92.8
MsaG035599 AT1G24190.2 34.682 173 106 6 3 172 4 172 2.48e-18 85.1
MsaG035599 AT1G24190.2 41.975 81 45 1 186 266 15 93 4.60e-11 63.5
MsaG035599 AT1G24190.1 37.037 162 96 5 91 250 6 163 6.10e-21 92.8
MsaG035599 AT1G24190.1 34.682 173 106 6 3 172 4 172 2.75e-18 85.1
MsaG035599 AT1G24190.1 41.975 81 45 1 186 266 15 93 4.64e-11 63.5
MsaG035599 AT1G24190.3 37.037 162 96 5 91 250 6 163 6.65e-21 92.8
MsaG035599 AT1G24190.3 34.682 173 106 6 3 172 4 172 2.68e-18 85.1
MsaG035599 AT1G24190.3 41.975 81 45 1 186 266 15 93 4.44e-11 63.5
MsaG035599 AT1G70030.1 34.395 157 93 4 96 249 8 157 2.93e-20 85.5
MsaG035599 AT1G70030.1 35.762 151 91 3 14 163 12 157 8.91e-20 84.3
MsaG035599 AT1G70060.2 34.731 167 96 5 90 250 5 164 3.89e-19 87.4
MsaG035599 AT1G70060.2 33.333 165 103 4 3 164 4 164 2.09e-17 82.4
MsaG035599 AT1G70060.1 34.731 167 96 5 90 250 5 164 3.89e-19 87.4
MsaG035599 AT1G70060.1 33.333 165 103 4 3 164 4 164 2.09e-17 82.4
MsaG035599 AT3G01320.2 30.435 207 136 4 47 249 2 204 3.00e-18 85.1
MsaG035599 AT3G01320.2 29.911 224 144 6 14 232 58 273 4.85e-18 84.3
MsaG035599 AT3G01320.2 48.485 66 34 0 201 266 71 136 5.40e-12 66.2
MsaG035599 AT3G01320.1 30.435 207 136 4 47 249 2 204 3.27e-18 84.7
MsaG035599 AT3G01320.1 29.911 224 144 6 14 232 58 273 5.86e-18 84.0
MsaG035599 AT3G01320.1 48.485 66 34 0 201 266 71 136 5.56e-12 66.2
MsaG035599 AT1G24230.1 25.410 244 153 6 3 241 4 223 5.02e-18 81.6
MsaG035599 AT1G24230.1 26.347 167 107 4 95 261 10 160 5.76e-12 64.7
MsaG035599 AT1G59890.1 33.088 136 89 1 115 250 59 192 1.79e-17 82.8
MsaG035599 AT1G59890.1 32.836 134 88 1 31 164 61 192 2.23e-15 76.6
MsaG035599 AT1G59890.4 33.088 136 89 1 115 250 59 192 1.83e-17 82.8
MsaG035599 AT1G59890.4 32.836 134 88 1 31 164 61 192 2.40e-15 76.3
MsaG035599 AT1G59890.3 33.088 136 89 1 115 250 59 192 1.87e-17 82.8
MsaG035599 AT1G59890.3 32.836 134 88 1 31 164 61 192 2.49e-15 76.3
MsaG035599 AT1G59890.2 33.088 136 89 1 115 250 59 192 1.93e-17 82.4
MsaG035599 AT1G59890.2 32.836 134 88 1 31 164 61 192 2.66e-15 76.3
MsaG035599 AT1G10450.3 33.577 137 89 1 117 253 100 234 1.34e-16 80.1
MsaG035599 AT1G10450.3 31.410 156 104 2 31 186 100 252 2.09e-15 76.6
MsaG035599 AT1G10450.2 33.577 137 89 1 117 253 49 183 1.71e-16 79.7
MsaG035599 AT1G10450.2 31.410 156 104 2 31 186 49 201 2.62e-15 76.3
MsaG035599 AT1G10450.1 33.577 137 89 1 117 253 49 183 1.71e-16 79.7
MsaG035599 AT1G10450.1 31.410 156 104 2 31 186 49 201 2.62e-15 76.3
MsaG035599 AT1G24200.1 30.519 154 102 4 96 249 11 159 1.03e-13 68.6
MsaG035599 AT5G35610.1 42.647 68 39 0 201 268 24 91 4.28e-11 60.5
MsaG035599 AT1G70030.2 28.025 157 71 4 96 249 19 136 4.36e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCAATTTAACGTGAAAT+TGG 0.263604 7:+16937551 None:intergenic
GGACCAATTTCACGTTAAAT+TGG 0.283882 7:-16937554 MsaT035599.1:CDS
ATAGATTTATTCAAGTTAAC+AGG 0.306329 7:-16938601 MsaT035599.1:CDS
AACATCATTGACAAATTCTT+TGG 0.313079 7:+16937908 None:intergenic
AGTAGTTCAATCTTGATATA+AGG 0.348020 7:+16938006 None:intergenic
TAGAATTGATGATGCAAATC+TGG 0.365588 7:-16937677 MsaT035599.1:CDS
TAACTTTCTGAATGTCTTGA+AGG 0.368309 7:-16938200 MsaT035599.1:CDS
AGAACATTGTGACGTCAAAA+AGG 0.380889 7:-16938651 MsaT035599.1:CDS
ATCTGAGGAGGAACAATCTC+TGG 0.416325 7:-16938308 MsaT035599.1:CDS
AACTTCATTGACAAATTCCT+TGG 0.417950 7:+16938628 None:intergenic
TAACTTTCTGAATGTGTTGA+AGG 0.435901 7:-16937844 MsaT035599.1:CDS
AAGGGATTATTTGAAGGTCA+TGG 0.456386 7:-16938388 MsaT035599.1:CDS
AAATCTTGGTGAATTATTGA+AGG 0.463354 7:-16938051 MsaT035599.1:CDS
AGCTTGATTTCAAATCCATC+AGG 0.489510 7:+16938334 None:intergenic
AAATCTCAGTAAATTCTTGA+AGG 0.490603 7:-16938407 MsaT035599.1:CDS
TTCTTGAAGGGATTATTTGA+AGG 0.491097 7:-16938394 MsaT035599.1:CDS
AGGATTGACGCTGCAAATCT+TGG 0.492061 7:-16938065 MsaT035599.1:CDS
AGCTTGATTTCATATCCATC+AGG 0.493606 7:+16937589 None:intergenic
AATCTTGGTGAATTATTGAA+GGG 0.505800 7:-16938050 MsaT035599.1:CDS
AGCTTGATTTCAAATCCATC+GGG 0.513708 7:+16937978 None:intergenic
TTATTGAAGGGATTATTTCA+AGG 0.513778 7:-16938038 MsaT035599.1:CDS
ATAATGAAGAAAATATTTGA+AGG 0.513952 7:-16937649 MsaT035599.1:CDS
TTGAACTACTTCATGCCTGA+TGG 0.515459 7:-16938349 MsaT035599.1:CDS
AATCTCAGTAAATTCTTGAA+GGG 0.520509 7:-16938406 MsaT035599.1:CDS
TGAAATCAAGCTTCCATTGG+AGG 0.531076 7:-16937578 MsaT035599.1:CDS
AAGCTTGATTTCAAATCCAT+CGG 0.533316 7:+16937977 None:intergenic
AGAGATTCTTCCTCCTCCGA+TGG 0.538054 7:+16937954 None:intergenic
TGAAATTGGTCCTCCTCCAA+TGG 0.554062 7:+16937565 None:intergenic
TTAAACTACTTCCTACCTGA+TGG 0.558050 7:-16937604 MsaT035599.1:CDS
AATCAAGCTTCCATCGGAGG+AGG 0.577026 7:-16937964 MsaT035599.1:CDS
ATATGAAATCAAGCTTCCAT+TGG 0.577817 7:-16937581 MsaT035599.1:CDS
ATTTATTCAAGTTAATCAGA+GGG 0.582516 7:-16937877 MsaT035599.1:CDS
TGATGCATTACAAGAAACAA+AGG 0.583155 7:-16938547 MsaT035599.1:intron
TGAAATCAAGCTTCCATCGG+AGG 0.587204 7:-16937967 MsaT035599.1:CDS
GATTTATTCAAGTTAATCAG+AGG 0.587296 7:-16937878 MsaT035599.1:CDS
GATTTATTCAAGTTAATCAG+AGG 0.587296 7:-16938234 MsaT035599.1:CDS
TGAAATCAAGCTTCTATCTG+AGG 0.589635 7:-16938323 MsaT035599.1:CDS
ATTTATTCAAGTTAATCAGA+GGG 0.591574 7:-16938233 MsaT035599.1:CDS
GATTTATTCAAGTTAACAGG+AGG 0.594070 7:-16938598 MsaT035599.1:CDS
TGAAGGATTACAAGAGACAA+AGG 0.594478 7:-16937827 MsaT035599.1:intron
TTGAACTACTTAATGCCCGA+TGG 0.600196 7:-16937993 MsaT035599.1:CDS
AATCAAGCTTCCATTGGAGG+AGG 0.611644 7:-16937575 MsaT035599.1:CDS
GGGCAACCCGTGATAATGGA+AGG 0.616033 7:-16938683 None:intergenic
ATTTATTCAAGTTAACAGGA+GGG 0.617938 7:-16938597 MsaT035599.1:CDS
ATTTGAAATCAAGCTTCCAT+CGG 0.624462 7:-16937970 MsaT035599.1:CDS
TGATTTCATATCCATCAGGT+AGG 0.626891 7:+16937593 None:intergenic
AATCAAGCTTCTATCTGAGG+AGG 0.631110 7:-16938320 MsaT035599.1:CDS
TGAAGGATTACAAAACACAA+AGG 0.632152 7:-16938183 MsaT035599.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 16937534 16938690 16937534 ID=MsaG035599
Chr7 mRNA 16937534 16938690 16937534 ID=MsaT035599.1;Parent=MsaG035599
Chr7 exon 16937534 16937696 16937534 ID=MsaT035599.1.exon4;Parent=MsaT035599.1
Chr7 CDS 16937534 16937696 16937534 ID=cds.MsaT035599.1;Parent=MsaT035599.1
Chr7 exon 16937828 16938085 16937828 ID=MsaT035599.1.exon3;Parent=MsaT035599.1
Chr7 CDS 16937828 16938085 16937828 ID=cds.MsaT035599.1;Parent=MsaT035599.1
Chr7 exon 16938184 16938441 16938184 ID=MsaT035599.1.exon2;Parent=MsaT035599.1
Chr7 CDS 16938184 16938441 16938184 ID=cds.MsaT035599.1;Parent=MsaT035599.1
Chr7 exon 16938548 16938690 16938548 ID=MsaT035599.1.exon1;Parent=MsaT035599.1
Chr7 CDS 16938548 16938690 16938548 ID=cds.MsaT035599.1;Parent=MsaT035599.1
Gene Sequence

>MsaT035599.1

ATGGAAGGACATTCTCAAGAACATTGTGACGTCAAAAAGGCCAAGGAATTTGTCAATGAAGTTAAGCATAGATTTATTCAAGTTAACAGGAGGGATAAGTATGATGACTTTTTGAATGTCTTGATGCATTACAAGAAACAAAGGATCGATGATGCAAATCTCAGTAAATTCTTGAAGGGATTATTTGAAGGTCATGGAGATCTTATATCAAGATTGAACTACTTCATGCCTGATGGATTTGAAATCAAGCTTCTATCTGAGGAGGAACAATCTCTGGAGCATTTTGTAGCCAAAAATGCGAAAGAATTTGTCAATGATGTTAAGTGTAGATTTATTCAAGTTAATCAGAGGGATAAGTATGATAACTTTCTGAATGTCTTGAAGGATTACAAAACACAAAGGATTGACGCTGCAAATCTTGGTGAATTATTGAAGGGATTATTTCAAGGTCATAGAGACCTTATATCAAGATTGAACTACTTAATGCCCGATGGATTTGAAATCAAGCTTCCATCGGAGGAGGAAGAATCTCTTGAACATTCTGAAGAAAAAACTGCCAAAGAATTTGTCAATGATGTTAAGTGTAGATTTATTCAAGTTAATCAGAGGGAAAAGTATGTTAACTTTCTGAATGTGTTGAAGGATTACAAGAGACAAAGAATTGATGATGCAAATCTGGTTAAAATAATGAAGAAAATATTTGAAGGTCATAGCGATTTAATATCAAGATTAAACTACTTCCTACCTGATGGATATGAAATCAAGCTTCCATTGGAGGAGGACCAATTTCACGTTAAATTGGAAGATGCTACAAGTTCTTGA

Protein sequence

>MsaT035599.1

MEGHSQEHCDVKKAKEFVNEVKHRFIQVNRRDKYDDFLNVLMHYKKQRIDDANLSKFLKGLFEGHGDLISRLNYFMPDGFEIKLLSEEEQSLEHFVAKNAKEFVNDVKCRFIQVNQRDKYDNFLNVLKDYKTQRIDAANLGELLKGLFQGHRDLISRLNYLMPDGFEIKLPSEEEESLEHSEEKTAKEFVNDVKCRFIQVNQREKYVNFLNVLKDYKRQRIDDANLVKIMKKIFEGHSDLISRLNYFLPDGYEIKLPLEEDQFHVKLEDATSS*