AlfalfaGEDB | MsaG035718

M. sativa ssp. caerulea / MsaG035718

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035718	AES95190.2	61.603	237	43	7	1	189	1	237	6.17e-69	220

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035718	sp\|Q84JK2\|FD_ARATH	56.250	80	30	1	110	189	200	274	7.60e-18	82.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035718	tr\|G7JXL9\|G7JXL9_MEDTR	61.603	237	43	7	1	189	1	237	2.94e-69	220

TFs/TRs:

Gene ID	Type	Classification
MsaG035718	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035718	MtrunA17_Chr5g0405951	61.603	237	43	7	1	189	1	237	5.67e-73	220
MsaG035718	MtrunA17_Chr3g0133971	46.316	95	37	3	88	182	324	404	1.97e-14	70.5
MsaG035718	MtrunA17_Chr7g0254991	35.533	197	89	9	13	184	116	299	3.00e-14	69.7
MsaG035718	MtrunA17_Chr4g0044771	36.986	146	54	6	37	182	246	353	2.46e-13	67.4
MsaG035718	MtrunA17_Chr3g0080341	44.715	123	44	6	61	176	192	297	1.94e-12	64.3
MsaG035718	MtrunA17_Chr8g0355121	67.308	52	12	1	125	176	242	288	2.97e-11	60.8
MsaG035718	MtrunA17_Chr2g0321411	60.345	58	15	2	125	182	210	259	2.98e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035718	AT4G35900.1	56.250	80	30	1	110	189	200	274	7.73e-19	82.0
MsaG035718	AT2G17770.2	39.568	139	59	5	59	189	63	184	8.10e-18	77.8
MsaG035718	AT1G45249.1	48.421	95	34	3	88	182	310	389	2.24e-15	73.6
MsaG035718	AT1G45249.7	48.421	95	34	3	88	182	325	404	2.45e-15	73.6
MsaG035718	AT1G45249.6	48.421	95	34	3	88	182	325	404	2.45e-15	73.6
MsaG035718	AT1G45249.3	45.545	101	39	3	88	182	310	400	2.18e-14	70.9
MsaG035718	AT3G56850.1	71.154	52	10	1	125	176	226	272	3.81e-13	66.6
MsaG035718	AT4G34000.1	43.564	101	38	3	83	182	343	425	5.54e-13	66.6
MsaG035718	AT4G34000.4	43.564	101	38	3	83	182	343	425	5.54e-13	66.6
MsaG035718	AT4G34000.2	43.564	101	38	3	83	182	343	425	5.54e-13	66.6
MsaG035718	AT2G41070.4	65.000	60	12	2	125	184	200	250	1.14e-12	65.1
MsaG035718	AT2G41070.3	65.000	60	12	2	125	184	191	241	1.16e-12	65.1
MsaG035718	AT2G41070.1	65.000	60	12	2	125	184	191	241	1.16e-12	65.1
MsaG035718	AT2G41070.2	65.000	60	12	2	125	184	191	241	1.16e-12	65.1
MsaG035718	AT2G41070.5	73.469	49	8	1	125	173	191	234	3.47e-12	63.5
MsaG035718	AT4G34000.3	44.681	94	33	3	83	175	343	418	4.42e-12	64.3
MsaG035718	AT3G19290.4	41.935	93	37	3	90	182	329	404	8.57e-12	63.2
MsaG035718	AT2G36270.3	62.963	54	15	1	125	178	356	404	1.27e-11	62.8
MsaG035718	AT2G36270.1	62.963	54	15	1	125	178	356	404	1.33e-11	62.8
MsaG035718	AT2G36270.2	62.963	54	15	1	125	178	356	404	1.33e-11	62.8
MsaG035718	AT3G19290.1	41.935	93	37	3	90	182	329	404	1.40e-11	62.8
MsaG035718	AT3G19290.5	41.935	93	37	3	90	182	329	404	1.40e-11	62.8
MsaG035718	AT3G19290.3	41.935	93	37	3	90	182	329	404	1.44e-11	62.8
MsaG035718	AT1G49720.2	50.725	69	28	2	111	178	297	360	3.98e-11	61.2
MsaG035718	AT1G49720.1	50.725	69	28	2	111	178	297	360	5.69e-11	60.8
MsaG035718	AT1G49720.3	50.725	69	28	2	111	178	297	360	5.69e-11	60.8

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAGAATAATCGATGCTTT+TGG	0.258209	7:+19555970	None:intergenic
TCTAATTCTTTCATTTCTTT+TGG	0.267657	7:-19555761	MsaT035718.1:CDS
AGTACTCGTAATTGTACTTT+TGG	0.310858	7:-19556043	MsaT035718.1:CDS
CCATGGAAGAGGTGTGGAAT+TGG	0.340299	7:-19556075	MsaT035718.1:CDS
CATTGAACTAGGGCAATAAA+AGG	0.373167	7:+19555898	None:intergenic
CCCTTGGTGCAGGAATGTTT+TGG	0.413920	7:+19555837	None:intergenic
ATAAGAGGATGATCAAGAAT+CGG	0.446318	7:-19555691	MsaT035718.1:CDS
AGAGCAGCAAGTTCAACTAT+TGG	0.448847	7:-19554936	MsaT035718.1:CDS
TTTGGGTGGGTCTGAAGTGA+GGG	0.456936	7:+19555988	None:intergenic
CTTGAGTGTGAAAGTCTTGA+AGG	0.469462	7:+19556011	None:intergenic
GATGGTGGGGTCATTGAACT+AGG	0.476787	7:+19555887	None:intergenic
CCAAAACATTCCTGCACCAA+GGG	0.498072	7:-19555837	MsaT035718.1:CDS
CTCGCAGAAACCCTTGGTGC+AGG	0.502817	7:+19555827	None:intergenic
TAAGAGGATGATCAAGAATC+GGG	0.505249	7:-19555690	MsaT035718.1:CDS
CCAATTCCACACCTCTTCCA+TGG	0.509593	7:+19556075	None:intergenic
TCTTATTTCTCCTTTCACTA+CGG	0.532558	7:+19555708	None:intergenic
GCGTTCACGAGCTAGAAAAC+AGG	0.535637	7:-19555657	MsaT035718.1:CDS
TTTACGCTCGCAGAAACCCT+TGG	0.536841	7:+19555821	None:intergenic
TGAAAATCAGTGACAAGAGA+TGG	0.550991	7:+19555869	None:intergenic
AAAGAAGTTAACCATGGAAG+AGG	0.555448	7:-19556086	None:intergenic
ATCAGTGACAAGAGATGGTG+GGG	0.566918	7:+19555874	None:intergenic
ACACCCACATTCTGATCATA+CGG	0.580440	7:+19555791	None:intergenic
CTGATATTAGCCGTAGTGAA+AGG	0.586903	7:-19555718	MsaT035718.1:CDS
AATCAGTGACAAGAGATGGT+GGG	0.594382	7:+19555873	None:intergenic
GCAGCAAGTTCAACTATTGG+AGG	0.594793	7:-19554933	MsaT035718.1:CDS
AGTTAACCATGGAAGAGGTG+TGG	0.606460	7:-19556081	None:intergenic
ACCAAAACATTCCTGCACCA+AGG	0.607070	7:-19555838	MsaT035718.1:CDS
AAATCAGTGACAAGAGATGG+TGG	0.615325	7:+19555872	None:intergenic
ACTTAGAAGACAGCAACAAG+AGG	0.615687	7:-19554897	MsaT035718.1:intron
TTCACCGTATGATCAGAATG+TGG	0.616125	7:-19555795	MsaT035718.1:CDS
ATGGTGGGGTCATTGAACTA+GGG	0.621945	7:+19555888	None:intergenic
TCACCGTATGATCAGAATGT+GGG	0.625766	7:-19555794	MsaT035718.1:CDS
GTAGTGAAAGGAGAAATAAG+AGG	0.627501	7:-19555706	MsaT035718.1:CDS
TTACGGGAAGCAGAAAGCGT+TGG	0.638395	7:-19553904	MsaT035718.1:CDS
ACATTCTGATCATACGGTGA+AGG	0.644907	7:+19555797	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	19553888	19556095	19553888	ID=MsaG035718
Chr7	mRNA	19553888	19556095	19553888	ID=MsaT035718.1;Parent=MsaG035718
Chr7	exon	19553888	19553926	19553888	ID=MsaT035718.1.exon3;Parent=MsaT035718.1
Chr7	CDS	19553888	19553926	19553888	ID=cds.MsaT035718.1;Parent=MsaT035718.1
Chr7	exon	19554898	19554975	19554898	ID=MsaT035718.1.exon2;Parent=MsaT035718.1
Chr7	CDS	19554898	19554975	19554898	ID=cds.MsaT035718.1;Parent=MsaT035718.1
Chr7	exon	19555643	19556095	19555643	ID=MsaT035718.1.exon1;Parent=MsaT035718.1
Chr7	CDS	19555643	19556095	19555643	ID=cds.MsaT035718.1;Parent=MsaT035718.1

Gene Sequence

>MsaT035718.1

ATGGAAGAGGTGTGGAATTGGAAATACATGAGTACTCGTAATTGTACTTTTGGTGGTGTTATCCTTCAAGACTTTCACACTCAAGCCCTCACTTCAGACCCACCCAAAAGCATCGATTATTCTTCTAATAATTTATCTTCCTTCGTTGCTTCAGATCAAAATAACAATAATGCTTCCTTTTATTGCCCTAGTTCAATGACCCCACCATCTCTTGTCACTGATTTTCACTTTGTTCACCAAAACATTCCTGCACCAAGGGTTTCTGCGAGCGTAAATCCTTCACCGTATGATCAGAATGTGGGTGTTGCTGCATCTAATTCTTTCATTTCTTTTGGTAAGAGATCTTGTGAAGCAACTGATATTAGCCGTAGTGAAAGGAGAAATAAGAGGATGATCAAGAATCGGGAATCTGCTGCGCGTTCACGAGCTAGAAAACAGGCAAAAATTCTTTCTGCTTACACGATTGAACTAGAGCAGCAAGTTCAACTATTGGAGGAAGAGAATGCAAGACTTAGAAGACAGCAACAAGAGTTACGGGAAGCAGAAAGCGTTGGTCAGCAAAAGAAATAG

Protein sequence

>MsaT035718.1

MEEVWNWKYMSTRNCTFGGVILQDFHTQALTSDPPKSIDYSSNNLSSFVASDQNNNNASFYCPSSMTPPSLVTDFHFVHQNIPAPRVSASVNPSPYDQNVGVAASNSFISFGKRSCEATDISRSERRNKRMIKNRESAARSRARKQAKILSAYTIELEQQVQLLEEENARLRRQQQELREAESVGQQKK*