AlfalfaGEDB | MsaG035781

M. sativa ssp. caerulea / MsaG035781

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035781	XP_013447699.1	99.387	163	1	0	1	163	29	191	3.29e-114	332

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035781	sp\|Q9FMC2\|BZP43_ARATH	57.042	142	56	3	11	148	20	160	2.19e-42	141

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035781	tr\|A0A072TWB0\|A0A072TWB0_MEDTR	99.387	163	1	0	1	163	29	191	1.57e-114	332

TFs/TRs:

Gene ID	Type	Classification
MsaG035781	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG007279	MsaG035781	0.800454	2.392071e-48	3.267598e-46
MsaG024877	MsaG035781	0.803440	5.864559e-49	8.575162e-47
MsaG027825	MsaG035781	0.801798	1.274375e-48	1.794542e-46
MsaG030595	MsaG035781	0.804310	3.875345e-49	5.782054e-47
MsaG035781	MsaG036882	0.820264	1.317992e-52	2.919216e-50
MsaG035781	MsaG044881	0.803617	5.390841e-49	7.914800e-47
MsaG035781	MsaG031490	0.827170	3.234495e-54	8.641594e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035781	MtrunA17_Chr7g0220571	99.387	163	1	0	1	163	29	191	3.03e-118	332
MsaG035781	MtrunA17_Chr6g0456061	73.050	141	34	3	13	149	41	181	5.09e-58	179
MsaG035781	MtrunA17_Chr7g0225661	64.179	134	43	4	32	162	57	188	9.47e-49	155
MsaG035781	MtrunA17_Chr8g0341311	59.864	147	52	6	19	162	35	177	1.50e-45	147
MsaG035781	MtrunA17_Chr4g0035414	52.326	86	38	2	42	127	16	98	1.07e-17	75.5
MsaG035781	MtrunA17_Chr1g0155551	44.330	97	54	0	33	129	4	100	4.68e-17	73.9
MsaG035781	MtrunA17_Chr3g0144941	50.000	80	40	0	48	127	19	98	1.50e-16	72.4
MsaG035781	MtrunA17_Chr4g0054291	33.974	156	85	3	9	146	7	162	2.51e-16	72.0
MsaG035781	MtrunA17_Chr1g0194681	37.405	131	70	4	34	158	7	131	7.29e-14	65.1
MsaG035781	MtrunA17_Chr5g0400901	37.705	122	71	2	28	144	25	146	7.46e-13	62.8
MsaG035781	MtrunA17_Chr5g0410531	37.736	106	61	2	26	126	272	377	2.65e-11	60.8
MsaG035781	MtrunA17_Chr8g0378901	32.292	96	65	0	32	127	8	103	5.67e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035781	AT3G30530.1	58.784	148	49	4	9	148	24	167	8.10e-48	153
MsaG035781	AT5G38800.1	57.042	142	56	3	11	148	20	160	2.23e-43	141
MsaG035781	AT2G04038.1	64.583	96	34	0	39	134	54	149	1.42e-36	124
MsaG035781	AT5G15830.1	61.616	99	38	0	37	135	52	150	3.83e-35	121
MsaG035781	AT1G13600.1	59.184	98	39	1	40	137	68	164	3.34e-34	119
MsaG035781	AT1G68880.1	44.348	115	59	1	23	137	16	125	9.58e-24	90.9
MsaG035781	AT5G60830.1	51.724	87	41	1	51	136	62	148	4.88e-19	80.5
MsaG035781	AT2G18160.1	50.000	72	36	0	56	127	28	99	1.54e-15	70.5
MsaG035781	AT3G62420.1	44.304	79	44	0	49	127	15	93	2.36e-15	69.3
MsaG035781	AT1G75390.1	40.187	107	61	1	24	127	3	109	2.44e-15	70.1
MsaG035781	AT1G75390.2	39.815	108	62	1	24	128	3	110	9.63e-15	67.0
MsaG035781	AT4G34590.1	45.946	74	40	0	54	127	22	95	1.98e-12	62.0

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGATTTGAATAGGGTTT+TGG	0.268357	7:-20899995	None:intergenic
TAGAATTGGTTGGAGAGTTT+TGG	0.269358	7:-20899963	None:intergenic
AAACTCTCCAACCAATTCTA+TGG	0.313604	7:+20899966	MsaT035781.1:CDS
GAAGAATCAGATTTAACATT+TGG	0.320345	7:-20900392	None:intergenic
AGCTTTGGTCACAGGTGGTT+TGG	0.335781	7:+20900180	MsaT035781.1:CDS
CTTTCAATTGAACATTCTCT+TGG	0.350205	7:-20900271	None:intergenic
GGTCACAGGTGGTTTGGCTT+AGG	0.364737	7:+20900186	MsaT035781.1:CDS
AAGTCTTGGAGGATTTGAAT+AGG	0.369300	7:-20900002	None:intergenic
GAGGTGTTTGGATGGTGTTT+TGG	0.378558	7:-20899935	None:intergenic
TTTGGTTGAAACCATAGAAT+TGG	0.382996	7:-20899977	None:intergenic
AGTCTTGGAGGATTTGAATA+GGG	0.412178	7:-20900001	None:intergenic
AGCTTCAGAACTTCGACAAA+TGG	0.463372	7:+20900298	MsaT035781.1:CDS
TGGATGTTGAGGTGTTTGGA+TGG	0.476729	7:-20899943	None:intergenic
TTTGTTTCCTCATTCGCGAC+CGG	0.491382	7:-20900145	None:intergenic
ACTTGATGAGCTTTGGTCAC+AGG	0.496643	7:+20900172	MsaT035781.1:CDS
AGGATGGAGGACTGAAGTCT+TGG	0.496834	7:-20900016	None:intergenic
ATGGAGGACTGAAGTCTTGG+AGG	0.500414	7:-20900013	None:intergenic
GCGACCGGCGTGCTGACTCT+CGG	0.500855	7:-20900130	None:intergenic
GTTGAAACCATAGAATTGGT+TGG	0.501172	7:-20899973	None:intergenic
CAGCACGCCGGTCGCGAATG+AGG	0.513300	7:+20900138	MsaT035781.1:CDS
CGAAAGAAAACATCGAAGAA+TGG	0.528388	7:+20900100	MsaT035781.1:CDS
ACATTTGGTGAAGTGATGCT+AGG	0.529651	7:-20900377	None:intergenic
TTCTTTCGTTGATTAGACCT+AGG	0.573011	7:-20900085	None:intergenic
ACATCTTCAAGAGGACTCAA+AGG	0.584334	7:-20900353	None:intergenic
TGATGAGCTTTGGTCACAGG+TGG	0.592404	7:+20900175	MsaT035781.1:CDS
TTGCTGCTTATGCATGAGGA+TGG	0.607001	7:-20900032	None:intergenic
CAACTCAACTTCCGACGAAG+CGG	0.609407	7:+20900052	MsaT035781.1:CDS
CTGCTTATGCATGAGGATGG+AGG	0.633472	7:-20900029	None:intergenic
ATGCTAGGAACATCTTCAAG+AGG	0.640644	7:-20900362	None:intergenic
TGAGTTGCTGCTTATGCATG+AGG	0.648721	7:-20900036	None:intergenic
GAAGCGGACGAACAAAACCT+AGG	0.674445	7:+20900068	MsaT035781.1:CDS
CGAACCGAGAGTCAGCACGC+CGG	0.746941	7:+20900126	MsaT035781.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	20899930	20900421	20899930	ID=MsaG035781
Chr7	mRNA	20899930	20900421	20899930	ID=MsaT035781.1;Parent=MsaG035781
Chr7	exon	20899930	20900421	20899930	ID=MsaT035781.1.exon1;Parent=MsaT035781.1
Chr7	CDS	20899930	20900421	20899930	ID=cds.MsaT035781.1;Parent=MsaT035781.1

Gene Sequence

>MsaT035781.1

ATGATCCAAAACACCATCCAAACACCTCAACATCCAAAACTCTCCAACCAATTCTATGGTTTCAACCAAAACCCTATTCAAATCCTCCAAGACTTCAGTCCTCCATCCTCATGCATAAGCAGCAACTCAACTTCCGACGAAGCGGACGAACAAAACCTAGGTCTAATCAACGAAAGAAAACATCGAAGAATGGTATCGAACCGAGAGTCAGCACGCCGGTCGCGAATGAGGAAACAAAAACAACTTGATGAGCTTTGGTCACAGGTGGTTTGGCTTAGGAATGAAAATCATCAATTACTAGATAAGCTCAATAATTTCTGTGAGACTCATGATAAAGTTGTCCAAGAGAATGTTCAATTGAAAGAACAAGCTTCAGAACTTCGACAAATGGTGTGTGATATGCAGTTACATAGTACTTGTCTTCCTTTGAGTCCTCTTGAAGATGTTCCTAGCATCACTTCACCAAATGTTAAATCTGATTCTTCAAACTAA

Protein sequence

>MsaT035781.1

MIQNTIQTPQHPKLSNQFYGFNQNPIQILQDFSPPSSCISSNSTSDEADEQNLGLINERKHRRMVSNRESARRSRMRKQKQLDELWSQVVWLRNENHQLLDKLNNFCETHDKVVQENVQLKEQASELRQMVCDMQLHSTCLPLSPLEDVPSITSPNVKSDSSN*