AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035798

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035798 MtrunA17_Chr7g0220721 100.000 109 0 0 1 109 288 396 4.13e-74 224
MsaG035798 MtrunA17_Chr4g0072871 49.606 127 43 4 1 109 252 375 8.52e-34 120
MsaG035798 MtrunA17_Chr1g0150471 54.545 110 39 4 1 108 215 315 7.63e-28 103
MsaG035798 MtrunA17_Chr3g0136701 53.211 109 25 4 1 108 206 289 3.48e-27 100
MsaG035798 MtrunA17_Chr8g0390971 70.455 44 13 0 40 83 358 401 1.23e-13 65.5
MsaG035798 MtrunA17_Chr7g0251781 63.043 46 17 0 39 84 340 385 1.41e-13 65.1
MsaG035798 MtrunA17_Chr1g0209861 69.767 43 13 0 40 82 382 424 1.92e-13 64.7
MsaG035798 MtrunA17_Chr2g0322951 63.043 46 17 0 36 81 88 133 2.22e-12 59.3
MsaG035798 MtrunA17_Chr3g0120711 62.791 43 16 0 39 81 353 395 2.67e-12 61.6
MsaG035798 MtrunA17_Chr4g0030421 46.296 54 29 0 32 85 27 80 2.90e-12 58.9
MsaG035798 MtrunA17_Chr7g0268691 55.319 47 21 0 35 81 323 369 8.82e-12 60.1
MsaG035798 MtrunA17_Chr5g0431351 65.116 43 15 0 40 82 174 216 1.57e-11 58.5
MsaG035798 MtrunA17_Chr7g0226451 62.791 43 16 0 40 82 309 351 1.72e-11 59.3
MsaG035798 MtrunA17_Chr2g0327621 44.231 52 29 0 35 86 150 201 6.13e-11 57.0
MsaG035798 MtrunA17_Chr2g0311591 39.286 84 43 3 27 104 158 239 6.45e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035798 AT3G02380.1 67.544 114 32 1 1 109 234 347 1.23e-42 142
MsaG035798 AT5G15850.1 66.667 114 30 3 1 109 245 355 3.37e-41 139
MsaG035798 AT5G15840.1 63.793 116 33 4 1 109 260 373 1.27e-40 138
MsaG035798 AT5G57660.1 54.464 112 42 3 1 109 250 355 2.98e-28 105
MsaG035798 AT5G24930.1 50.926 108 47 2 2 109 261 362 1.03e-26 101
MsaG035798 AT2G24790.1 61.905 84 23 2 1 84 199 273 6.80e-25 95.5
MsaG035798 AT1G73870.1 66.000 50 17 0 36 85 341 390 4.76e-14 67.0
MsaG035798 AT1G68520.1 43.529 85 47 1 1 85 319 402 1.19e-13 65.9
MsaG035798 AT2G33500.2 63.043 46 17 0 39 84 355 400 1.55e-13 65.5
MsaG035798 AT2G33500.1 63.043 46 17 0 39 84 356 401 1.55e-13 65.5
MsaG035798 AT1G25440.1 44.186 86 29 1 16 82 318 403 5.22e-13 63.9
MsaG035798 AT2G47890.1 51.852 54 26 0 31 84 278 331 5.36e-13 63.9
MsaG035798 AT5G14370.1 47.619 63 33 0 22 84 276 338 1.66e-12 62.4
MsaG035798 AT3G07650.3 53.571 56 25 1 27 81 301 356 6.20e-12 60.8
MsaG035798 AT3G07650.2 53.571 56 25 1 27 81 301 356 6.20e-12 60.8
MsaG035798 AT3G07650.4 53.571 56 25 1 27 81 301 356 6.20e-12 60.8
MsaG035798 AT3G07650.1 53.571 56 25 1 27 81 301 356 6.20e-12 60.8
MsaG035798 AT3G21880.1 60.417 48 19 0 37 84 304 351 6.77e-12 60.8
MsaG035798 AT1G49130.2 62.222 45 17 0 39 83 270 314 7.45e-12 60.5
MsaG035798 AT1G49130.1 62.222 45 17 0 39 83 277 321 7.84e-12 60.5
MsaG035798 AT3G21880.3 60.417 48 19 0 37 84 308 355 8.36e-12 60.5
MsaG035798 AT3G21880.2 60.417 48 19 0 37 84 308 355 8.36e-12 60.5
MsaG035798 AT1G07050.1 62.222 45 17 0 38 82 149 193 2.50e-11 57.8
MsaG035798 AT2G33350.5 34.146 82 52 1 14 93 291 372 2.54e-11 59.3
MsaG035798 AT2G33350.4 34.146 82 52 1 14 93 271 352 3.98e-11 58.5
MsaG035798 AT2G33350.2 34.146 82 52 1 14 93 282 363 4.00e-11 58.5
MsaG035798 AT5G57180.2 50.000 62 21 1 21 82 374 425 4.17e-11 58.5
MsaG035798 AT4G25990.1 48.438 64 23 1 19 82 330 383 4.33e-11 58.5
MsaG035798 AT2G33350.1 34.146 82 52 1 14 93 283 364 4.41e-11 58.5
MsaG035798 AT1G28050.1 54.348 46 21 0 39 84 384 429 6.84e-11 57.8
MsaG035798 AT5G48250.1 54.717 53 22 1 29 81 307 357 9.47e-11 57.4
MsaG035798 AT5G41380.1 47.059 51 27 0 35 85 210 260 9.51e-11 57.4

Find 19 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGGTCAATTGTTCCTTT+TGG 0.257022 7:+21388803 None:intergenic
GAATAGGTCATGGTTGATTC+TGG 0.297791 7:+21388833 None:intergenic
GAAACTCGGCCGCGTATAAA+AGG 0.298232 7:-21388644 MsaT035798.1:CDS
GAAAATTTGAGAAAACAATT+AGG 0.345101 7:-21388691 MsaT035798.1:CDS
TTTAACATCATTTCAGAAAG+AGG 0.365033 7:+21388532 None:intergenic
CTAATTACAGAAGTTGGATA+TGG 0.366492 7:-21388563 MsaT035798.1:CDS
TGGGAAGACATTTGAATAGA+AGG 0.393236 7:+21388773 None:intergenic
GAATAGAAGGACCTGAAAAT+AGG 0.406008 7:+21388786 None:intergenic
ACATCACTAATTACAGAAGT+TGG 0.453991 7:-21388569 MsaT035798.1:CDS
ACTCTTGCTTCTCTATCCAT+TGG 0.478486 7:+21388743 None:intergenic
TCAGTTTCATCAATGGATGT+TGG 0.481745 7:-21388863 None:intergenic
TGGAGGTCTTGAATAGGTCA+TGG 0.494897 7:+21388823 None:intergenic
AAACAATTAGGTATGCTTCA+AGG 0.513566 7:-21388679 MsaT035798.1:CDS
TTAACATCATTTCAGAAAGA+GGG 0.529345 7:+21388533 None:intergenic
ACCTATTCAAGACCTCCAAA+AGG 0.546280 7:-21388818 MsaT035798.1:CDS
AACTTCTGTAATTAGTGATG+TGG 0.558143 7:+21388571 None:intergenic
ATAGAGAAGCAAGAGTCCTA+AGG 0.578755 7:-21388736 MsaT035798.1:CDS
ACGCGGCCGAGTTTCAGCAT+AGG 0.616320 7:+21388652 None:intergenic
GGAAAGCCTATGCTGAAACT+CGG 0.623923 7:-21388658 MsaT035798.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 21388544 21388873 21388544 ID=MsaG035798
Chr7 mRNA 21388544 21388873 21388544 ID=MsaT035798.1;Parent=MsaG035798
Chr7 exon 21388544 21388873 21388544 ID=MsaT035798.1.exon1;Parent=MsaT035798.1
Chr7 CDS 21388544 21388873 21388544 ID=cds.MsaT035798.1;Parent=MsaT035798.1
Gene Sequence

>MsaT035798.1

ATGGATGTTGGTGTTGTACCAGAATCAACCATGACCTATTCAAGACCTCCAAAAGGAACAATTGACCTATTTTCAGGTCCTTCTATTCAAATGTCTTCCCATTTTTCTCCAATGGATAGAGAAGCAAGAGTCCTAAGGTACAGAGAGAAAAAGAAGACAAGAAAATTTGAGAAAACAATTAGGTATGCTTCAAGGAAAGCCTATGCTGAAACTCGGCCGCGTATAAAAGGTCGATTCGCGAAGAGAACAGATGTAGAAGCTGAAGTTGATCAAATGTTTTCCACATCACTAATTACAGAAGTTGGATATGGAATTGTTCCCTCTTTCTGA
Protein sequence

>MsaT035798.1

MDVGVVPESTMTYSRPPKGTIDLFSGPSIQMSSHFSPMDREARVLRYREKKKTRKFEKTIRYASRKAYAETRPRIKGRFAKRTDVEAEVDQMFSTSLITEVGYGIVPSF*