AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035799 MtrunA17_Chr7g0220721 79.930 284 15 4 1 249 1 277 7.17e-117 340
MsaG035799 MtrunA17_Chr4g0072871 39.759 166 68 6 14 175 47 184 1.41e-24 100
MsaG035799 MtrunA17_Chr3g0136701 41.667 120 50 2 24 143 38 137 2.09e-22 93.6
MsaG035799 MtrunA17_Chr1g0150471 42.105 114 42 3 24 136 38 128 5.70e-20 87.0
MsaG035799 MtrunA17_Chr4g0033641 39.474 76 40 1 35 104 5 80 3.34e-12 64.3
MsaG035799 MtrunA17_Chr2g0329511 38.158 76 41 1 35 104 5 80 1.38e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035799 AT3G02380.1 44.492 236 62 9 24 251 48 222 1.62e-45 156
MsaG035799 AT5G15840.2 43.348 233 89 7 24 248 52 249 2.60e-42 145
MsaG035799 AT5G15840.1 43.348 233 89 7 24 248 52 249 6.72e-42 147
MsaG035799 AT5G15850.1 43.277 238 77 7 24 249 44 235 6.76e-34 125
MsaG035799 AT5G57660.1 37.313 201 81 8 27 226 53 209 1.38e-25 103
MsaG035799 AT2G24790.2 39.583 144 61 5 24 164 40 160 4.15e-24 96.7
MsaG035799 AT5G24930.1 34.583 240 107 8 24 249 38 241 4.74e-24 99.4
MsaG035799 AT2G24790.1 39.583 144 61 5 24 164 40 160 1.10e-23 97.4
MsaG035799 AT1G06040.2 38.158 76 41 1 35 104 5 80 5.17e-11 60.5
MsaG035799 AT2G31380.1 38.462 78 38 2 35 104 5 80 7.93e-11 60.8

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCGATTCTTCCAATTTC+CGG 0.213967 7:-21391916 MsaT035799.1:CDS
CTCATCATGATGAGTATTAT+TGG 0.214592 7:+21391570 None:intergenic
CTTCATCTTCGGCACCTAAA+AGG 0.221370 7:+21391869 None:intergenic
CCAATTTCCGGTTACCTTTA+CGG 0.243807 7:-21391904 MsaT035799.1:CDS
CCTCGAGGCACGAACGCGTC+TGG 0.290131 7:-21392062 MsaT035799.1:CDS
CCACGTTGCCGCAGACCTTA+TGG 0.314996 7:+21392198 None:intergenic
TTGTTATTGATGTTCTTTAG+AGG 0.325161 7:+21391733 None:intergenic
ATGAAAATGAAGCTGCTTCA+TGG 0.340820 7:-21391768 MsaT035799.1:CDS
AGCCGCCGGGGCACGCTCGC+AGG 0.350184 7:+21392029 None:intergenic
GGCTTTGTCGGCGGTGGTTG+TGG 0.350451 7:-21391847 MsaT035799.1:CDS
CATAATCAAGAGGGTAATAA+TGG 0.353323 7:-21391676 MsaT035799.1:CDS
CCGTAAAGGTAACCGGAAAT+TGG 0.358200 7:+21391904 None:intergenic
CAACAACAACAAGATCATTA+TGG 0.365626 7:-21391535 MsaT035799.1:CDS
TAACCGGAAATTGGAAGAAT+CGG 0.415877 7:+21391913 None:intergenic
GAGTTTGAGTCTTCCAAAGC+TGG 0.426808 7:-21391433 MsaT035799.1:CDS
GGTGGCTGTGGCTGAGGTGG+TGG 0.428121 7:+21392219 None:intergenic
GTCGGCGGTGGTTGTGGTGG+CGG 0.428147 7:-21391841 MsaT035799.1:CDS
GAAGAATCGGGACACGTTGA+TGG 0.431550 7:+21391926 None:intergenic
CCTTATGGTGGCTGTGGCTG+AGG 0.436720 7:+21392213 None:intergenic
ATTGTAACTGAATCCAGCTT+TGG 0.441148 7:+21391420 None:intergenic
TGTACCTGCTGTTGTTGAAC+AGG 0.442029 7:+21391475 None:intergenic
TGAAGGTGTTGATCATGATA+TGG 0.443702 7:-21391794 MsaT035799.1:CDS
GATGAAGGCTTTGTCGGCGG+TGG 0.445280 7:-21391853 MsaT035799.1:CDS
TTCATCTTCGGCACCTAAAA+GGG 0.458034 7:+21391870 None:intergenic
GGCCTGCGAGCGTGCCCCGG+CGG 0.459298 7:-21392031 MsaT035799.1:CDS
GCCGACAAAGCCTTCATCTT+CGG 0.465052 7:+21391858 None:intergenic
TGAGGCCTGCGAGCGTGCCC+CGG 0.465827 7:-21392034 MsaT035799.1:CDS
CTCGAGGCACGAACGCGTCT+GGG 0.468750 7:-21392061 MsaT035799.1:CDS
TGGAGAAGTTGATGAGTACT+TGG 0.471287 7:-21391641 MsaT035799.1:CDS
GCCGAAGATGAAGGCTTTGT+CGG 0.477058 7:-21391859 MsaT035799.1:CDS
CGGAGGACCGTAAAGGTAAC+CGG 0.478457 7:+21391897 None:intergenic
GTGGAAGAAGAGGAGGATGA+AGG 0.479258 7:-21391811 MsaT035799.1:CDS
TTGTGGTGGCGGCTGTGAGG+TGG 0.496832 7:-21391830 MsaT035799.1:CDS
CGCAGACCTTATGGTGGCTG+TGG 0.501654 7:+21392207 None:intergenic
CGGTTACCTTTACGGTCCTC+CGG 0.504333 7:-21391896 MsaT035799.1:CDS
CCTCAGCCACAGCCACCATA+AGG 0.513892 7:-21392213 MsaT035799.1:CDS
AACCGGAAATTGGAAGAATC+GGG 0.527364 7:+21391914 None:intergenic
CTTTACAAAGAAAAGCCGCC+GGG 0.532716 7:+21392016 None:intergenic
TATGGTGGCTGTGGCTGAGG+TGG 0.539082 7:+21392216 None:intergenic
TGGTTGTGGTGGCGGCTGTG+AGG 0.542306 7:-21391833 MsaT035799.1:CDS
CTGTGGCTGAGGTGGTGGTG+AGG 0.545540 7:+21392224 None:intergenic
CGTTGATGGCGGCTTGCAAG+AGG 0.550196 7:+21391940 None:intergenic
TTTGTCGGCGGTGGTTGTGG+TGG 0.554712 7:-21391844 MsaT035799.1:CDS
CTTGTTGATTGCAATTCTTG+TGG 0.560089 7:-21391616 MsaT035799.1:CDS
TCGGCACCTAAAAGGGTGGC+CGG 0.561181 7:+21391877 None:intergenic
CGGCTGTGAGGTGGAAGAAG+AGG 0.566525 7:-21391821 MsaT035799.1:CDS
GTTGATTGCAATTCTTGTGG+TGG 0.567048 7:-21391613 MsaT035799.1:CDS
CATAAGGTCTGCGGCAACGT+GGG 0.569294 7:-21392197 MsaT035799.1:CDS
TGTTCCTGTTCAACAACAGC+AGG 0.577069 7:-21391479 MsaT035799.1:CDS
GCTTTACAAAGAAAAGCCGC+CGG 0.590036 7:+21392015 None:intergenic
AATAACAACAACATTAGCAA+TGG 0.607541 7:-21391700 MsaT035799.1:CDS
CTGTGAGGTGGAAGAAGAGG+AGG 0.610586 7:-21391818 MsaT035799.1:CDS
TAGCAATGGTCATAATCAAG+AGG 0.613653 7:-21391686 MsaT035799.1:CDS
GGGTGGCCGGAGGACCGTAA+AGG 0.615076 7:+21391890 None:intergenic
CCATAAGGTCTGCGGCAACG+TGG 0.618452 7:-21392198 MsaT035799.1:CDS
AAAGCTGGATTCAGTTACAA+TGG 0.625769 7:-21391418 MsaT035799.1:CDS
AGCAATGGTCATAATCAAGA+GGG 0.627143 7:-21391685 MsaT035799.1:CDS
CACAGCCACCATAAGGTCTG+CGG 0.627512 7:-21392206 MsaT035799.1:CDS
GTTGATGGCGGCTTGCAAGA+GGG 0.631032 7:+21391941 None:intergenic
GTGGATATCAGCATCACAAG+TGG 0.632088 7:+21391972 None:intergenic
ATCTTCGGCACCTAAAAGGG+TGG 0.644787 7:+21391873 None:intergenic
TTGCAAGAGGGTTAGCAGAG+TGG 0.655218 7:+21391953 None:intergenic
GAAGATGAAGGCTTTGTCGG+CGG 0.655301 7:-21391856 MsaT035799.1:CDS
CACAAGAATTGCAATCAACA+AGG 0.655647 7:+21391617 None:intergenic
CGAACGCGTCTGGGTCTGTG+AGG 0.656001 7:-21392052 MsaT035799.1:CDS
GCTGGATTCAGTTACAATGG+TGG 0.656212 7:-21391415 MsaT035799.1:CDS
GAATCGGGACACGTTGATGG+CGG 0.662257 7:+21391929 None:intergenic
GCACCTAAAAGGGTGGCCGG+AGG 0.665880 7:+21391880 None:intergenic
CGTTGCCGCAGACCTTATGG+TGG 0.670034 7:+21392201 None:intergenic
CGGCAAACCGTGTAGCCTCG+AGG 0.686538 7:-21392077 MsaT035799.1:intron
TTTACAAAGAAAAGCCGCCG+GGG 0.712447 7:+21392017 None:intergenic
CCAGACGCGTTCGTGCCTCG+AGG 0.721870 7:+21392062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 21391300 21392263 21391300 ID=MsaG035799
Chr7 mRNA 21391300 21392263 21391300 ID=MsaT035799.1;Parent=MsaG035799
Chr7 exon 21391300 21391305 21391300 ID=MsaT035799.1.exon3;Parent=MsaT035799.1
Chr7 CDS 21391300 21391305 21391300 ID=cds.MsaT035799.1;Parent=MsaT035799.1
Chr7 exon 21391399 21392084 21391399 ID=MsaT035799.1.exon2;Parent=MsaT035799.1
Chr7 CDS 21391399 21392084 21391399 ID=cds.MsaT035799.1;Parent=MsaT035799.1
Chr7 exon 21392188 21392263 21392188 ID=MsaT035799.1.exon1;Parent=MsaT035799.1
Chr7 CDS 21392188 21392263 21392188 ID=cds.MsaT035799.1;Parent=MsaT035799.1
Gene Sequence

>MsaT035799.1

ATGTTGGAGCAAGATTTCCTCACCACCACCTCAGCCACAGCCACCATAAGGTCTGCGGCAACGTGGGCACGAGCATCCTCGAGGCACGAACGCGTCTGGGTCTGTGAGGCCTGCGAGCGTGCCCCGGCGGCTTTTCTTTGTAAAGCTGATGCTGCTTCTCTTTGCTCCACTTGTGATGCTGATATCCACTCTGCTAACCCTCTTGCAAGCCGCCATCAACGTGTCCCGATTCTTCCAATTTCCGGTTACCTTTACGGTCCTCCGGCCACCCTTTTAGGTGCCGAAGATGAAGGCTTTGTCGGCGGTGGTTGTGGTGGCGGCTGTGAGGTGGAAGAAGAGGAGGATGAAGGTGTTGATCATGATATGGAAGATGAAAATGAAGCTGCTTCATGGCTTTTGTTGAATCCTCTAAAGAACATCAATAACAATAGTAACAACAATAACAACAACATTAGCAATGGTCATAATCAAGAGGGTAATAATGGATTTTTGTTTAGTGGAGAAGTTGATGAGTACTTGGACCTTGTTGATTGCAATTCTTGTGGTGGTGATGAAAACACTTTCAATACCAATAATACTCATCATGATGAGTATAGTCAACAACAACAACAACAAGATCATTATGGTGTTCCTCAAAAAAGCTATGTGGGTGATAGTGTTGTTCCTGTTCAACAACAGCAGGTACAGAATTTTCAACTTGGGTTGGAGTTTGAGTCTTCCAAAGCTGGATTCAGTTACAATGGTGGTTCTATTAGTCAAAGTGGTTGA
Protein sequence

>MsaT035799.1

MLEQDFLTTTSATATIRSAATWARASSRHERVWVCEACERAPAAFLCKADAASLCSTCDADIHSANPLASRHQRVPILPISGYLYGPPATLLGAEDEGFVGGGCGGGCEVEEEEDEGVDHDMEDENEAASWLLLNPLKNINNNSNNNNNNISNGHNQEGNNGFLFSGEVDEYLDLVDCNSCGGDENTFNTNNTHHDEYSQQQQQQDHYGVPQKSYVGDSVVPVQQQQVQNFQLGLEFESSKAGFSYNGGSISQSG*