AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG035844


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035844 MtrunA17_Chr1g0203051 57.721 272 109 1 1 272 1 266 9.45e-103 320
MsaG035844 MtrunA17_Chr8g0389811 42.391 184 88 2 30 196 13 195 3.64e-45 156
MsaG035844 MtrunA17_Chr2g0331371 39.881 168 87 2 34 193 2 163 3.61e-32 122
MsaG035844 MtrunA17_Chr6g0473421 40.000 135 67 2 34 160 2 130 9.87e-28 107
MsaG035844 MtrunA17_Chr2g0287651 29.703 202 136 2 76 273 66 265 3.15e-27 115
MsaG035844 MtrunA17_Chr1g0171741 33.158 190 119 3 33 215 74 262 1.55e-26 107
MsaG035844 MtrunA17_Chr1g0172931 42.727 110 63 0 58 167 49 158 6.84e-22 92.0
MsaG035844 MtrunA17_Chr8g0340801 51.389 72 35 0 196 267 1 72 2.50e-20 85.9
MsaG035844 MtrunA17_Chr2g0290391 31.169 154 102 3 97 247 63 215 1.38e-17 85.5
MsaG035844 MtrunA17_Chr1g0198761 30.556 180 108 6 61 224 16 194 9.74e-16 79.7
MsaG035844 MtrunA17_Chr2g0276841 35.433 127 77 4 97 219 82 207 6.62e-14 73.9
MsaG035844 MtrunA17_Chr8g0366311 47.458 59 31 0 58 116 52 110 2.23e-12 63.5
MsaG035844 MtrunA17_Chr7g0228061 44.828 87 43 3 373 455 5 90 4.03e-12 62.4
MsaG035844 MtrunA17_Chr5g0448761 26.882 186 127 4 91 270 66 248 1.63e-11 66.6
MsaG035844 MtrunA17_Chr5g0431001 30.435 138 86 3 96 224 72 208 2.16e-11 65.9
MsaG035844 MtrunA17_Chr6g0458391 25.131 191 113 7 97 261 103 289 3.05e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG035844 AT5G28530.4 32.374 139 91 2 97 233 67 204 1.72e-19 91.7
MsaG035844 AT5G28530.1 29.474 190 117 5 97 271 67 254 1.85e-19 91.7
MsaG035844 AT5G28530.3 29.474 190 117 5 97 271 67 254 1.87e-19 91.7
MsaG035844 AT5G28530.2 29.474 190 117 5 97 271 67 254 1.87e-19 91.7
MsaG035844 AT5G28530.5 32.374 139 91 2 97 233 67 204 2.04e-19 91.7
MsaG035844 AT1G10240.2 30.851 188 110 9 97 273 60 238 7.24e-13 71.2
MsaG035844 AT1G10240.1 30.851 188 110 9 97 273 60 238 7.24e-13 71.2

Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGCATCTTTAGCACTTT+TGG 0.220681 7:+22535032 None:intergenic
CGTTCCATCACAACAGTTTC+CGG 0.225407 7:+22535060 None:intergenic
CCTCCACATACTCCACATTT+AGG 0.288554 7:+22534688 None:intergenic
CTTTCCTCATTGCTTTCATT+TGG 0.313855 7:+22536312 None:intergenic
TTTGTCATAGAGAAGGTTTC+CGG 0.338339 7:-22536547 MsaT035844.1:CDS
CCAGCGACGGGGAGAAGTTT+AGG 0.341691 7:-22534796 MsaT035844.1:CDS
CCTTGAGAAAATTAATTTCA+AGG 0.345881 7:-22536723 MsaT035844.1:CDS
TTTGGTGTGGGTCCAGCGAC+GGG 0.348536 7:-22534808 MsaT035844.1:CDS
ATTCCTACGTCACGTATCTA+TGG 0.365780 7:-22536284 MsaT035844.1:CDS
GCGTTCCTCGGATGATGATA+TGG 0.366309 7:-22536813 MsaT035844.1:CDS
GGTTGTGTGCACGGATTTGC+CGG 0.389576 7:-22536626 MsaT035844.1:CDS
AGCTAGTAGGTGTGGGTGTA+TGG 0.394921 7:-22536477 MsaT035844.1:CDS
TATACACAAAACCATTGAAA+TGG 0.395440 7:-22534887 MsaT035844.1:CDS
TATGAACATAAGAAAATTGC+CGG 0.401136 7:-22535079 MsaT035844.1:CDS
CTTGGACAGTGGCTCTTCTT+CGG 0.429033 7:-22536846 MsaT035844.1:CDS
TTGTCATAGAGAAGGTTTCC+GGG 0.431471 7:-22536546 MsaT035844.1:CDS
CTCAGAAACTGATTATGAAC+AGG 0.434115 7:-22534614 MsaT035844.1:CDS
ATCCCGGTGACTTGAACTAT+TGG 0.441196 7:+22534996 None:intergenic
TCAAATGTGAGCAACATAAC+GGG 0.446705 7:-22537029 MsaT035844.1:CDS
GTTTGGTGTGGGTCCAGCGA+CGG 0.454406 7:-22534809 MsaT035844.1:CDS
CTTGAATTGCTCATTGTACA+TGG 0.461269 7:+22536208 None:intergenic
TGTGGAGTATGTGGAGGAAA+GGG 0.464409 7:-22534682 MsaT035844.1:CDS
GGTCAAGTTGATGAGGTGTT+TGG 0.465868 7:-22534826 MsaT035844.1:CDS
AACTACGTATGTTATGTTTG+TGG 0.474919 7:-22534962 MsaT035844.1:CDS
TGCATTTCGAGTTGAAGAAT+CGG 0.477767 7:+22534735 None:intergenic
TAGGTAATCCGCAGCGGCGT+AGG 0.486624 7:-22534777 MsaT035844.1:CDS
GCTGGTGGATTCAGACATCT+AGG 0.489368 7:-22536245 MsaT035844.1:CDS
TTTATGATGACAATGACGAT+TGG 0.491653 7:-22534588 MsaT035844.1:CDS
TCACTCATCTTTCTATGTGC+AGG 0.492964 7:+22536347 None:intergenic
GAGAGCAGAAGCCAGCTAGT+AGG 0.495906 7:-22536490 MsaT035844.1:CDS
CCTAAACTTCTCCCCGTCGC+TGG 0.500888 7:+22534796 None:intergenic
TTCAAATGTGAGCAACATAA+CGG 0.501010 7:-22537030 MsaT035844.1:CDS
GGAAAGTGTCGTGCGTTCCT+CGG 0.501486 7:-22536825 MsaT035844.1:CDS
ATGAAAGCAATGAGGAAAGC+TGG 0.503092 7:-22536308 MsaT035844.1:CDS
TGACGATTGGAACGTTCCTA+TGG 0.504057 7:-22534575 MsaT035844.1:intron
GACACGTACAATTCGACGTA+TGG 0.507400 7:-22536788 MsaT035844.1:CDS
ATGCCAATAGTTCAAGTCAC+CGG 0.516528 7:-22534999 MsaT035844.1:CDS
CCGCAGCGGCGTAGGAAAAG+AGG 0.517276 7:-22534769 MsaT035844.1:CDS
CAGAAGCCAGCTAGTAGGTG+TGG 0.527642 7:-22536485 MsaT035844.1:CDS
ATGTGGAGTATGTGGAGGAA+AGG 0.529949 7:-22534683 MsaT035844.1:CDS
TATTGTCATTCCTTGCATCC+CGG 0.530083 7:+22536528 None:intergenic
ACAATGAGCAATTCAAGGAT+AGG 0.534325 7:-22536202 MsaT035844.1:CDS
ACGCCTAAATGTGGAGTATG+TGG 0.534842 7:-22534691 MsaT035844.1:CDS
CAATGAGCAATTCAAGGATA+GGG 0.537667 7:-22536201 MsaT035844.1:CDS
AACAACACGGGATCTTCTAC+CGG 0.539063 7:+22536607 None:intergenic
CATGTACAATGAGCAATTCA+AGG 0.539489 7:-22536207 MsaT035844.1:CDS
GTTGATGAGGTGTTTGGTGT+GGG 0.541519 7:-22534820 MsaT035844.1:CDS
ACCTTTGTTTGTCATAGAGA+AGG 0.542041 7:-22536554 MsaT035844.1:CDS
GGAAGCAATTGAATTTCTGA+AGG 0.545431 7:-22536156 MsaT035844.1:CDS
AATAGGTACGGTTGTGTGCA+CGG 0.547142 7:-22536635 MsaT035844.1:CDS
GACGAAGAGTTCTGATGCAG+AGG 0.547324 7:-22536177 MsaT035844.1:CDS
GAAGGCATCGTAGGTGTGGA+AGG 0.550437 7:-22537005 MsaT035844.1:CDS
AGTTGATGAGGTGTTTGGTG+TGG 0.553811 7:-22534821 MsaT035844.1:CDS
ACATACGTAGTTACAAAAGC+AGG 0.568046 7:+22534973 None:intergenic
TAGTTACAAAAGCAGGATCC+CGG 0.574230 7:+22534980 None:intergenic
GAAGCAATTGAATTTCTGAA+GGG 0.574630 7:-22536155 MsaT035844.1:CDS
ATTCAAGTTCACGTTGACTA+CGG 0.576810 7:-22536449 MsaT035844.1:CDS
CGTAGGTGTGGAAGGTTCAG+AGG 0.578719 7:-22536997 MsaT035844.1:intron
GACATCTAGGATATTCAAGA+AGG 0.580326 7:-22536232 MsaT035844.1:CDS
AGAAGCCAGCTAGTAGGTGT+GGG 0.582078 7:-22536484 MsaT035844.1:CDS
TGCCAATAGTTCAAGTCACC+GGG 0.582672 7:-22534998 MsaT035844.1:CDS
GAGAAGGTTTCCGGGATGCA+AGG 0.585476 7:-22536538 MsaT035844.1:CDS
GTGTCATGTCGGTTCCGTCA+CGG 0.585630 7:-22534649 MsaT035844.1:CDS
ACACGTACAATTCGACGTAT+GGG 0.592382 7:-22536787 MsaT035844.1:CDS
ATTGCCGGAAACTGTTGTGA+TGG 0.595496 7:-22535064 MsaT035844.1:CDS
AGTCTATAGTTGCTCCGTGA+CGG 0.595691 7:+22534635 None:intergenic
AAACGGAACACGCCTAAATG+TGG 0.598130 7:-22534700 MsaT035844.1:CDS
ATTCTACAGGACTTGGACAG+TGG 0.602899 7:-22536857 MsaT035844.1:intron
TGGATTGCCACACTTTAACA+TGG 0.603575 7:+22534915 None:intergenic
GACATTGGAATCATTCAACA+TGG 0.604480 7:-22534848 MsaT035844.1:CDS
CATGCTTGTTCTTAACAACA+CGG 0.605806 7:+22536594 None:intergenic
TGTGGAGCGTTGCAAGAGGA+TGG 0.606817 7:-22534944 MsaT035844.1:CDS
ATACACCCACACCTACTAGC+TGG 0.608717 7:+22536479 None:intergenic
CGTGTTGTTAAGAACAAGCA+TGG 0.609169 7:-22536593 MsaT035844.1:CDS
CAACATGGGTCAAGTTGATG+AGG 0.613553 7:-22534833 MsaT035844.1:CDS
CAAATGTGAGCAACATAACG+GGG 0.613878 7:-22537028 MsaT035844.1:CDS
ACCTTCTCTATGACAAACAA+AGG 0.615884 7:+22536553 None:intergenic
AAGCAATTGAATTTCTGAAG+GGG 0.619311 7:-22536154 MsaT035844.1:CDS
ATGCTTGTTCTTAACAACAC+GGG 0.628391 7:+22536595 None:intergenic
TTGGTGTGGGTCCAGCGACG+GGG 0.643872 7:-22534807 MsaT035844.1:CDS
GTCACAACCGCGTGTCATGT+CGG 0.644843 7:-22534660 MsaT035844.1:CDS
GTCTTCCATATCATCATCCG+AGG 0.646493 7:+22536808 None:intergenic
GCAAACGCCATGTTAAAGTG+TGG 0.657355 7:-22534922 MsaT035844.1:CDS
TGTTTGTGGAGCGTTGCAAG+AGG 0.662336 7:-22534948 MsaT035844.1:CDS
CAGCGGCGTAGGAAAAGAGG+CGG 0.664927 7:-22534766 MsaT035844.1:CDS
GACGGAACCGACATGACACG+CGG 0.668500 7:+22534653 None:intergenic
AATACCAAATGAAAGCAATG+AGG 0.671984 7:-22536316 MsaT035844.1:CDS
CCTAAATGTGGAGTATGTGG+AGG 0.673219 7:-22534688 MsaT035844.1:CDS
ACGTTGACTACGGTAGTGAA+CGG 0.682166 7:-22536439 MsaT035844.1:CDS
AAACTGTTGTGATGGAACGA+TGG 0.688506 7:-22535056 MsaT035844.1:CDS
AAACCATAGATACGTGACGT+AGG 0.700125 7:+22536281 None:intergenic
ACATTGGAATCATTCAACAT+GGG 0.703646 7:-22534847 MsaT035844.1:CDS
GAAGTTTAGGTAATCCGCAG+CGG 0.723333 7:-22534783 MsaT035844.1:CDS
AAATGTGAGCAACATAACGG+GGG 0.740353 7:-22537027 MsaT035844.1:CDS
TTGACTACGGTAGTGAACGG+TGG 0.752541 7:-22536436 MsaT035844.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 22534466 22537087 22534466 ID=MsaG035844
Chr7 mRNA 22534466 22537087 22534466 ID=MsaT035844.1;Parent=MsaG035844
Chr7 exon 22534466 22534486 22534466 ID=MsaT035844.1.exon4;Parent=MsaT035844.1
Chr7 CDS 22534466 22534486 22534466 ID=cds.MsaT035844.1;Parent=MsaT035844.1
Chr7 exon 22534576 22535114 22534576 ID=MsaT035844.1.exon3;Parent=MsaT035844.1
Chr7 CDS 22534576 22535114 22534576 ID=cds.MsaT035844.1;Parent=MsaT035844.1
Chr7 exon 22536147 22536870 22536147 ID=MsaT035844.1.exon2;Parent=MsaT035844.1
Chr7 CDS 22536147 22536870 22536147 ID=cds.MsaT035844.1;Parent=MsaT035844.1
Chr7 exon 22536998 22537087 22536998 ID=MsaT035844.1.exon1;Parent=MsaT035844.1
Chr7 CDS 22536998 22537087 22536998 ID=cds.MsaT035844.1;Parent=MsaT035844.1
Gene Sequence

>MsaT035844.1

ATGGATATGAGTGAAATTCTTGAAATTTTTCAAGTTTCAAATGTGAGCAACATAACGGGGGAAGGCATCGTAGGTGTGGAAGGTTCAGAGGACTTGGACAGTGGCTCTTCTTCGGAAAGTGTCGTGCGTTCCTCGGATGATGATATGGAAGACACGTACAATTCGACGTATGGGTGCAGTTTTGATGATGAATTTGCGGTTGATTGCATGACTGACCTTGAGAAAATTAATTTCAAGGAATTAAGTTATGATGACTTTATGATGTACCATTTTTCGGATCGTCTAGTTGCATTCAAGTTTTACAATAGGTACGGTTGTGTGCACGGATTTGCCGGTAGAAGATCCCGTGTTGTTAAGAACAAGCATGGCGATGCTATTCAACAAACCTTTGTTTGTCATAGAGAAGGTTTCCGGGATGCAAGGAATGACAATAATCCGTCTAGAAAAAGAGAGCAGAAGCCAGCTAGTAGGTGTGGGTGTATGGCAAGAATTCAAGTTCACGTTGACTACGGTAGTGAACGGTGGTACATAAAAATGTTTGACGATGTTCATAATCACTCATTTTTGGATAATAAGTATGAGGGGATGCTACCTGCACATAGAAAGATGAGTGATTATGACAAATACCAAATGAAAGCAATGAGGAAAGCTGGCATTCCTACGTCACGTATCTATGGTTTTTTTGCTTCACAAGCTGGTGGATTCAGACATCTAGGATATTCAAGAAGGACCATGTACAATGAGCAATTCAAGGATAGGGAGACGAAGAGTTCTGATGCAGAGGAAGCAATTGAATTTCTGAAGGGGTTGTCTAGTGTTTTAGTGCATATGAACATAAGAAAATTGCCGGAAACTGTTGTGATGGAACGATGGACCAAAAGTGCTAAAGATGCAATCAATGCACAAAATGCCAATAGTTCAAGTCACCGGGATCCTGCTTTTGTAACTACGTATGTTATGTTTGTGGAGCGTTGCAAGAGGATGGCAAACGCCATGTTAAAGTGTGGCAATCCAGATTATATACACAAAACCATTGAAATGGTTGAAAAACACACTGAGACATTGGAATCATTCAACATGGGTCAAGTTGATGAGGTGTTTGGTGTGGGTCCAGCGACGGGGAGAAGTTTAGGTAATCCGCAGCGGCGTAGGAAAAGAGGCGGAGCAACAGCCGATTCTTCAACTCGAAATGCAAATGCGAAGAAAAAACGGAACACGCCTAAATGTGGAGTATGTGGAGGAAAGGGTCACAACCGCGTGTCATGTCGGTTCCGTCACGGAGCAACTATAGACTCAGAAACTGATTATGAACAGGAATTTTATGATGACAATGACGATTGGAACGTTCCTATGGAGGACTTGAATTACGTGTGA

Protein sequence

>MsaT035844.1

MDMSEILEIFQVSNVSNITGEGIVGVEGSEDLDSGSSSESVVRSSDDDMEDTYNSTYGCSFDDEFAVDCMTDLEKINFKELSYDDFMMYHFSDRLVAFKFYNRYGCVHGFAGRRSRVVKNKHGDAIQQTFVCHREGFRDARNDNNPSRKREQKPASRCGCMARIQVHVDYGSERWYIKMFDDVHNHSFLDNKYEGMLPAHRKMSDYDKYQMKAMRKAGIPTSRIYGFFASQAGGFRHLGYSRRTMYNEQFKDRETKSSDAEEAIEFLKGLSSVLVHMNIRKLPETVVMERWTKSAKDAINAQNANSSSHRDPAFVTTYVMFVERCKRMANAMLKCGNPDYIHKTIEMVEKHTETLESFNMGQVDEVFGVGPATGRSLGNPQRRRKRGGATADSSTRNANAKKKRNTPKCGVCGGKGHNRVSCRFRHGATIDSETDYEQEFYDDNDDWNVPMEDLNYV*