AlfalfaGEDB | MsaG035902

M. sativa ssp. caerulea / MsaG035902

KEGG: -
GO: GO:0002237 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0042221 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0071216 GO:0071219 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF13837.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035902	XP_003621623.1	99.413	341	2	0	1	341	1	341	0.0	695

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035902	sp\|Q8VZ20\|ASR3_ARATH	48.295	352	133	9	1	341	1	314	4.60e-95	288

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035902	tr\|G7KSV7\|G7KSV7_MEDTR	99.413	341	2	0	1	341	1	341	0.0	695

TFs/TRs:

Gene ID	Type	Classification
MsaG035902	TF	Trihelix

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035902	MtrunA17_Chr7g0221451	99.123	342	2	1	1	341	1	342	0.0	692
MsaG035902	MtrunA17_Chr1g0211061	67.147	347	77	9	1	341	1	316	5.38e-139	397
MsaG035902	MtrunA17_Chr8g0385751	36.232	345	155	12	36	341	43	361	7.73e-56	186
MsaG035902	MtrunA17_Chr1g0199044	29.730	148	64	5	41	148	56	203	9.79e-12	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG035902	AT2G33550.1	48.295	352	133	9	1	341	1	314	4.68e-96	288
MsaG035902	AT4G31270.1	33.913	115	70	1	39	153	17	125	8.38e-17	80.1
MsaG035902	AT2G35640.1	34.711	121	62	5	41	148	22	138	2.53e-12	67.4

Find 118 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGATGTAGAGCCCGTTTC+TGG	0.174990	7:+23998827	None:intergenic
CCTTCACCTGTTCCTATATC+AGG	0.202031	7:-24001606	MsaT035902.1:intron
ACAGTTCCATCATCGGAATT+AGG	0.210777	7:+24002239	None:intergenic
TTGGACTAGGCAGGAGATTT+TGG	0.244674	7:-24002141	MsaT035902.1:CDS
GATAAAGGAGTGGGAGAGTC+AGG	0.272993	7:-24001937	MsaT035902.1:CDS
AAACCGGGTCGGAATGGGTC+TGG	0.281955	7:-24002080	MsaT035902.1:CDS
CAGTTCCATCATCGGAATTA+GGG	0.288650	7:+24002240	None:intergenic
TTCATATTTATGATAGTAAT+AGG	0.301580	7:-24001707	MsaT035902.1:CDS
GGCTGGTTGTTTCTCATTGT+TGG	0.301660	7:+23998856	None:intergenic
TTTCATTCACTTTATGGCTT+TGG	0.307015	7:-24002273	None:intergenic
GGTCGGAATGGGTCTGGGTT+TGG	0.340314	7:-24002074	MsaT035902.1:CDS
GAATGTGTCCGTGTCGGTTT+CGG	0.345627	7:-24001754	MsaT035902.1:CDS
CTCTTCATTGCCGTCTCCAT+TGG	0.349024	7:+23998775	None:intergenic
TTCCTTCTCCTTGGCAGCCT+CGG	0.354622	7:+24000860	None:intergenic
CAAAATAACCAACCTGATAT+AGG	0.358309	7:+24001594	None:intergenic
GGCAGCATCGGTTGTTGTTC+CGG	0.362479	7:-24001778	MsaT035902.1:CDS
TCCATCCTCGCCACCGTTCA+CGG	0.362719	7:+24002187	None:intergenic
TCCGATGATGGAACTGTTAA+CGG	0.379041	7:-24002233	MsaT035902.1:CDS
GGCTGCAGCTGTGGCAGCAT+CGG	0.381376	7:-24001790	MsaT035902.1:CDS
CATTCCGACCCGGTTTGAAC+CGG	0.381786	7:+24002087	None:intergenic
GAAGAAACCAACGCCCATTT+CGG	0.382737	7:+24002041	None:intergenic
GAGATGAAACGGAGTCGTTT+TGG	0.385379	7:-24001911	MsaT035902.1:CDS
TTGCAGTTTCATTCTGCTGC+TGG	0.394241	7:+23998651	None:intergenic
CCCGTTTCTGGATTCGAGGC+TGG	0.399420	7:+23998839	None:intergenic
GTAAGAAACACGGTGTGAAC+CGG	0.402207	7:-24002013	MsaT035902.1:CDS
TAGTCAATTAATTGATGTAT+TGG	0.403614	7:-23998738	MsaT035902.1:CDS
AACCGGGTCGGAATGGGTCT+GGG	0.403971	7:-24002079	MsaT035902.1:CDS
TTGGTTCGAGTGAACCGAAA+TGG	0.407548	7:-24002055	MsaT035902.1:CDS
CCATCATCGGAATTAGGGTT+TGG	0.414944	7:+24002245	None:intergenic
TGCTGAAAGCCGGTTCAAAC+CGG	0.422601	7:-24002096	MsaT035902.1:CDS
GATGCTAAGTGAACAATTAG+AGG	0.427523	7:-23998702	MsaT035902.1:CDS
CGGAGATTATAAGAAGATAA+AGG	0.430532	7:-24001952	MsaT035902.1:CDS
CTTGAGCATTTCCTTCTCCT+TGG	0.433995	7:+24000851	None:intergenic
CTTTATCTTCTTATAATCTC+CGG	0.440339	7:+24001953	None:intergenic
GATAAGCTTGCAAATGCTCT+TGG	0.446812	7:-23998608	MsaT035902.1:CDS
GCCGTCGATGGCGGTGGCAG+CGG	0.449844	7:-24001811	MsaT035902.1:CDS
CACCGCTTCCGACACTGAAT+CGG	0.450184	7:+24001987	None:intergenic
GTCCGTGTCGGTTTCGGAGA+CGG	0.458922	7:-24001748	MsaT035902.1:CDS
AGGAAAGTGAGTGGTGAGGA+TGG	0.468097	7:-24001687	MsaT035902.1:CDS
CATCTCAAGGGGAACGTAAG+AGG	0.469277	7:-23998811	MsaT035902.1:CDS
CGGTTCAAACCGGGTCGGAA+TGG	0.469834	7:-24002086	MsaT035902.1:CDS
TCGGTTTCGGAGACGGTTGG+AGG	0.471862	7:-24001741	MsaT035902.1:CDS
GGTTCAAACCGGGTCGGAAT+GGG	0.472434	7:-24002085	MsaT035902.1:CDS
GGAGACGGTTGGAGGGGATG+AGG	0.473755	7:-24001733	MsaT035902.1:CDS
GCCGCTGCCACCGCCATCGA+CGG	0.475194	7:+24001810	None:intergenic
CCAAACCCTAATTCCGATGA+TGG	0.478565	7:-24002245	MsaT035902.1:CDS
TGGTTCGAGTGAACCGAAAT+GGG	0.480374	7:-24002054	MsaT035902.1:CDS
AACCAACCTGATATAGGAAC+AGG	0.482028	7:+24001600	None:intergenic
ACCAGCCTCGAATCCAGAAA+CGG	0.487810	7:-23998840	MsaT035902.1:CDS
GCCGTGAACGGTGGCGAGGA+TGG	0.493423	7:-24002188	MsaT035902.1:CDS
GGTGGCAGCGGCTGCAGCTG+TGG	0.495633	7:-24001799	MsaT035902.1:CDS
ACCGACACGGACACATTCAC+CGG	0.507822	7:+24001759	None:intergenic
CGAGGCTGCCACGTTGGACT+AGG	0.508267	7:-24002154	MsaT035902.1:CDS
GGAATGATTTGAGGAGAGAG+AGG	0.508729	7:-24001881	MsaT035902.1:CDS
GAGTCAGGTGAGAGATGAAA+CGG	0.509162	7:-24001922	MsaT035902.1:CDS
GCTGCCACGTTGGACTAGGC+AGG	0.510527	7:-24002150	MsaT035902.1:CDS
CCACTCCTCCGAGGCTGCCA+AGG	0.511072	7:-24000868	MsaT035902.1:CDS
GGTTGATGAGGAATGATTTG+AGG	0.515241	7:-24001890	MsaT035902.1:CDS
CCAGCCTCGAATCCAGAAAC+GGG	0.515344	7:-23998839	MsaT035902.1:CDS
TAAGAAACACGGTGTGAACC+GGG	0.516907	7:-24002012	MsaT035902.1:CDS
CTCCGAGGCTGCCAAGGAGA+AGG	0.523413	7:-24000862	MsaT035902.1:CDS
CGGTTTCGGAGACGGTTGGA+GGG	0.523976	7:-24001740	MsaT035902.1:CDS
TGATTCGCCGTCGATGGCGG+TGG	0.527873	7:-24001817	MsaT035902.1:CDS
CGATTCAGTGTCGGAAGCGG+TGG	0.529001	7:-24001986	MsaT035902.1:CDS
AGGAGAGAGAGGAAATTGCC+TGG	0.529803	7:-24001870	MsaT035902.1:CDS
AGAGCCCGTTTCTGGATTCG+AGG	0.531658	7:+23998835	None:intergenic
CCACCGTTCACGGCGGATGA+CGG	0.533916	7:+24002197	None:intergenic
ACCGTTAACAGTTCCATCAT+CGG	0.535210	7:+24002232	None:intergenic
GATACTTGATTCGCCGTCGA+TGG	0.535395	7:-24001823	MsaT035902.1:CDS
TTATAAGAAGATAAAGGAGT+GGG	0.535976	7:-24001946	MsaT035902.1:CDS
GTGTCGGTTTCGGAGACGGT+TGG	0.540037	7:-24001744	MsaT035902.1:CDS
GAAACGGGCTCTACATCTCA+AGG	0.544821	7:-23998824	MsaT035902.1:CDS
GTCCGATTCAGTGTCGGAAG+CGG	0.545969	7:-24001989	MsaT035902.1:CDS
CTTCTCCTTGGCAGCCTCGG+AGG	0.552038	7:+24000863	None:intergenic
ATTATAAGAAGATAAAGGAG+TGG	0.554162	7:-24001947	MsaT035902.1:CDS
AAGGGGAACGTAAGAGGAAG+CGG	0.555390	7:-23998805	MsaT035902.1:CDS
GGTTTCGGAGACGGTTGGAG+GGG	0.562864	7:-24001739	MsaT035902.1:CDS
GAAAGAGTGATGCTGAAAGC+CGG	0.563160	7:-24002106	MsaT035902.1:CDS
AACCCAGACCCATTCCGACC+CGG	0.563556	7:+24002077	None:intergenic
CCTTGGCAGCCTCGGAGGAG+TGG	0.564966	7:+24000868	None:intergenic
GACGGTTGGAGGGGATGAGG+AGG	0.566380	7:-24001730	MsaT035902.1:CDS
AGGAAGCGGTTCGCAACCAA+TGG	0.566583	7:-23998791	MsaT035902.1:CDS
CAACGTGGCAGCCTCGCCGT+TGG	0.571589	7:+24002161	None:intergenic
TCCGACACTGAATCGGACCC+CGG	0.576178	7:+24001994	None:intergenic
CAACGGCGAGGCTGCCACGT+TGG	0.578495	7:-24002160	MsaT035902.1:CDS
GCTGAAAGCCGGTTCAAACC+GGG	0.579950	7:-24002095	MsaT035902.1:CDS
CCACCGTCATCCGCCGTGAA+CGG	0.582016	7:-24002200	MsaT035902.1:CDS
GAGTGAACCGAAATGGGCGT+TGG	0.586856	7:-24002048	MsaT035902.1:CDS
AAGCGGTGGAGCAACCTCGC+CGG	0.587972	7:-24001972	MsaT035902.1:CDS
ATCCGCCGTGAACGGTGGCG+AGG	0.594053	7:-24002192	MsaT035902.1:CDS
TCCGGTGAATGTGTCCGTGT+CGG	0.602281	7:-24001760	MsaT035902.1:CDS
CAATGGAGACGGCAATGAAG+AGG	0.604269	7:-23998774	MsaT035902.1:CDS
GGAAAGTGAGTGGTGAGGAT+GGG	0.605368	7:-24001686	MsaT035902.1:CDS
CCGTCATCCGCCGTGAACGG+TGG	0.609094	7:-24002197	MsaT035902.1:CDS
TAATAGGAAAGTGAGTGGTG+AGG	0.611943	7:-24001691	MsaT035902.1:CDS
GGAGAGAGAGGAAATTGCCT+GGG	0.612198	7:-24001869	MsaT035902.1:CDS
ACTTGATTCGCCGTCGATGG+CGG	0.613112	7:-24001820	MsaT035902.1:CDS
CGGTTCGCAACCAATGGAGA+CGG	0.613454	7:-23998785	MsaT035902.1:CDS
AAACGGGCTCTACATCTCAA+GGG	0.623139	7:-23998823	MsaT035902.1:CDS
CAAGGAGAAGGAAATGCTCA+AGG	0.623679	7:-24000850	MsaT035902.1:intron
AACCGTCTCCGAAACCGACA+CGG	0.629472	7:+24001746	None:intergenic
GATGGAACTGTTAACGGTAA+CGG	0.629880	7:-24002227	MsaT035902.1:CDS
CCGTTCACGGCGGATGACGG+TGG	0.635532	7:+24002200	None:intergenic
CCTGATATAGGAACAGGTGA+AGG	0.639915	7:+24001606	None:intergenic
AACGGGCTCTACATCTCAAG+GGG	0.641332	7:-23998822	MsaT035902.1:CDS
TGGAGACGGCAATGAAGAGG+AGG	0.641529	7:-23998771	MsaT035902.1:CDS
TCTTCGTATTGTAAGAAACA+CGG	0.643626	7:-24002023	MsaT035902.1:CDS
ACCGGGGTCCGATTCAGTGT+CGG	0.645672	7:-24001995	MsaT035902.1:CDS
GATAGTAATAGGAAAGTGAG+TGG	0.654357	7:-24001696	MsaT035902.1:CDS
ACCTCCTTATCAAAATACCC+AGG	0.654771	7:+24001852	None:intergenic
TGGCGAGGATGGAAAACCAA+CGG	0.670801	7:-24002177	MsaT035902.1:CDS
TCTTCTTATAATCTCCGGCG+AGG	0.675112	7:+24001958	None:intergenic
AAGAAACACGGTGTGAACCG+GGG	0.696776	7:-24002011	MsaT035902.1:CDS
AAAGCCGGTTCAAACCGGGT+CGG	0.703772	7:-24002091	MsaT035902.1:CDS
AGGATGGAAAACCAACGGCG+AGG	0.725001	7:-24002172	MsaT035902.1:CDS
ATCTCCTGCCTAGTCCAACG+TGG	0.727740	7:+24002146	None:intergenic
ATCCTCGCCACCGTTCACGG+CGG	0.730721	7:+24002190	None:intergenic
CAGTTTCTACCACTCCTCCG+AGG	0.732051	7:-24000877	MsaT035902.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	23998586	24002282	23998586	ID=MsaG035902
Chr7	mRNA	23998586	24002282	23998586	ID=MsaT035902.1;Parent=MsaG035902
Chr7	exon	23998586	23998881	23998586	ID=MsaT035902.1.exon3;Parent=MsaT035902.1
Chr7	CDS	23998586	23998881	23998586	ID=cds.MsaT035902.1;Parent=MsaT035902.1
Chr7	exon	24000851	24000904	24000851	ID=MsaT035902.1.exon2;Parent=MsaT035902.1
Chr7	CDS	24000851	24000904	24000851	ID=cds.MsaT035902.1;Parent=MsaT035902.1
Chr7	exon	24001607	24002282	24001607	ID=MsaT035902.1.exon1;Parent=MsaT035902.1
Chr7	CDS	24001607	24002282	24001607	ID=cds.MsaT035902.1;Parent=MsaT035902.1

Gene Sequence

>MsaT035902.1

ATGGCTTTGGAACAACCAAACCCTAATTCCGATGATGGAACTGTTAACGGTAACGGAACACCACCGTCATCCGCCGTGAACGGTGGCGAGGATGGAAAACCAACGGCGAGGCTGCCACGTTGGACTAGGCAGGAGATTTTGGTTTTGATACAGGGAAAGAGTGATGCTGAAAGCCGGTTCAAACCGGGTCGGAATGGGTCTGGGTTTGGTTCGAGTGAACCGAAATGGGCGTTGGTTTCTTCGTATTGTAAGAAACACGGTGTGAACCGGGGTCCGATTCAGTGTCGGAAGCGGTGGAGCAACCTCGCCGGAGATTATAAGAAGATAAAGGAGTGGGAGAGTCAGGTGAGAGATGAAACGGAGTCGTTTTGGTTGATGAGGAATGATTTGAGGAGAGAGAGGAAATTGCCTGGGTATTTTGATAAGGAGGTTTATGAGATACTTGATTCGCCGTCGATGGCGGTGGCAGCGGCTGCAGCTGTGGCAGCATCGGTTGTTGTTCCGGTGAATGTGTCCGTGTCGGTTTCGGAGACGGTTGGAGGGGATGAGGAGGTTCATATTTATGATAGTAATAGGAAAGTGAGTGGTGAGGATGGGTTGTTTTCTGATTTTGAGAAGGATGAGGTTTTGGTGGCTAATAAGAATGTTCATGTTCCTTCACCTGTTCCTATATCAGAGAAGCAGTTTCTACCACTCCTCCGAGGCTGCCAAGGAGAAGGAAATGCTCAAGGTACCAACAATGAGAAACAACCAGCCTCGAATCCAGAAACGGGCTCTACATCTCAAGGGGAACGTAAGAGGAAGCGGTTCGCAACCAATGGAGACGGCAATGAAGAGGAGGAAAGTTTGCATAGTCAATTAATTGATGTATTGGAGAAAAATGGGAAGATGCTAAGTGAACAATTAGAGGCTCAGAACATCAATTTTCAGTTGGATCGCCAGCAGCAGAATGAAACTGCAAGTAGCATAGTTGCTGTGCTTGATAAGCTTGCAAATGCTCTTGGAAGAATTGCTGATAAGCTGTAG

Protein sequence

>MsaT035902.1

MALEQPNPNSDDGTVNGNGTPPSSAVNGGEDGKPTARLPRWTRQEILVLIQGKSDAESRFKPGRNGSGFGSSEPKWALVSSYCKKHGVNRGPIQCRKRWSNLAGDYKKIKEWESQVRDETESFWLMRNDLRRERKLPGYFDKEVYEILDSPSMAVAAAAAVAASVVVPVNVSVSVSETVGGDEEVHIYDSNRKVSGEDGLFSDFEKDEVLVANKNVHVPSPVPISEKQFLPLLRGCQGEGNAQGTNNEKQPASNPETGSTSQGERKRKRFATNGDGNEEEESLHSQLIDVLEKNGKMLSEQLEAQNINFQLDRQQQNETASSIVAVLDKLANALGRIADKL*