Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG035969 | AES77954.1 | 89.524 | 210 | 22 | 0 | 1 | 210 | 1 | 210 | 4.43e-138 | 396 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG035969 | tr|G7KUQ7|G7KUQ7_MEDTR | 89.524 | 210 | 22 | 0 | 1 | 210 | 1 | 210 | 2.12e-138 | 396 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG035969 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG035969 | MtrunA17_Chr7g0255511 | 74.312 | 218 | 51 | 2 | 1 | 218 | 1 | 213 | 3.30e-119 | 338 |
| MsaG035969 | MtrunA17_Chr7g0222361 | 88.439 | 173 | 20 | 0 | 38 | 210 | 1 | 173 | 1.18e-115 | 328 |
| MsaG035969 | MtrunA17_Chr7g0225121 | 74.479 | 192 | 44 | 2 | 1 | 192 | 1 | 187 | 3.02e-101 | 292 |
| MsaG035969 | MtrunA17_Chr7g0225091 | 67.143 | 210 | 50 | 3 | 1 | 210 | 1 | 191 | 1.23e-98 | 286 |
| MsaG035969 | MtrunA17_Chr7g0222371 | 84.298 | 121 | 19 | 0 | 23 | 143 | 1 | 121 | 8.69e-72 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATGACTGAGATTGAATT+TGG | 0.189345 | 7:-25208116 | MsaT035969.1:CDS |
| TTGAATTCTTAGTTTCAAAA+TGG | 0.214469 | 7:+25208588 | None:intergenic |
| ATTCTTCATCATCTTCAAAT+TGG | 0.223485 | 7:+25208655 | None:intergenic |
| CTCCTTCCCCTTGTTCTCAT+TGG | 0.229008 | 7:+25208700 | None:intergenic |
| CAAATTTGTGAGAACTTTGA+TGG | 0.256763 | 7:+25208399 | None:intergenic |
| AAGAAATGGAGGAATTTCTT+TGG | 0.278435 | 7:+25208520 | None:intergenic |
| TCTTGAGTCTCATTGTCTTC+TGG | 0.296217 | 7:+25208055 | None:intergenic |
| ACATATAGATGAAGTGAATA+AGG | 0.341762 | 7:-25208168 | MsaT035969.1:CDS |
| CACTGCCACTCCTTTCCATA+TGG | 0.342482 | 7:-25208291 | MsaT035969.1:CDS |
| GATTGAATTTGGTTCAATTT+CGG | 0.345163 | 7:-25208105 | MsaT035969.1:CDS |
| AGAAATGGAGGAATTTCTTT+GGG | 0.353550 | 7:+25208521 | None:intergenic |
| TCCAAGCTTCGTTAAAAGCT+TGG | 0.373123 | 7:+25208351 | None:intergenic |
| AATTACTCAAATGGTAATCA+AGG | 0.414383 | 7:-25208326 | MsaT035969.1:CDS |
| TGAATTCTTAGTTTCAAAAT+GGG | 0.419158 | 7:+25208589 | None:intergenic |
| ACTCCTTTCCATATGGGTTG+TGG | 0.424172 | 7:-25208284 | MsaT035969.1:CDS |
| TTCACTACTCCACGTGCATT+AGG | 0.424339 | 7:-25208425 | MsaT035969.1:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7:-25208242 | MsaT035969.1:CDS |
| ATTGATATCCACAACCCATA+TGG | 0.434960 | 7:+25208276 | None:intergenic |
| GCTTGGATAAATTACTCAAA+TGG | 0.446981 | 7:-25208335 | MsaT035969.1:CDS |
| TCACAAATTTGAGAGAAGTC+TGG | 0.482572 | 7:-25208387 | MsaT035969.1:CDS |
| ACTGCCACTCCTTTCCATAT+GGG | 0.486001 | 7:-25208290 | MsaT035969.1:CDS |
| TTATTCACTTCATCTATATG+TGG | 0.494069 | 7:+25208170 | None:intergenic |
| ATTTGGTTCAATTTCGGAGG+AGG | 0.516489 | 7:-25208099 | MsaT035969.1:CDS |
| TTGGATTCATTGTTGATCAG+AGG | 0.552579 | 7:-25208493 | MsaT035969.1:CDS |
| GAAGTGAATAAGGCTGATCA+AGG | 0.555258 | 7:-25208158 | MsaT035969.1:CDS |
| TTCATTGTTGATCAGAGGAA+AGG | 0.555662 | 7:-25208488 | MsaT035969.1:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7:-25208241 | MsaT035969.1:CDS |
| GTGCAACACCAATGAGAACA+AGG | 0.569603 | 7:-25208708 | MsaT035969.1:CDS |
| TGAATTTGGTTCAATTTCGG+AGG | 0.572045 | 7:-25208102 | MsaT035969.1:CDS |
| CAAGCGAAAGGCCATCGTGA+TGG | 0.579476 | 7:-25208618 | MsaT035969.1:CDS |
| ATTACTCAAATGGTAATCAA+GGG | 0.589859 | 7:-25208325 | MsaT035969.1:CDS |
| TGAGAACAAGGGGAAGGAGA+AGG | 0.594646 | 7:-25208696 | MsaT035969.1:CDS |
| CACCAATGAGAACAAGGGGA+AGG | 0.620535 | 7:-25208702 | MsaT035969.1:CDS |
| TATCCACAACCCATATGGAA+AGG | 0.633718 | 7:+25208281 | None:intergenic |
| ACTACTCCACGTGCATTAGG+TGG | 0.640042 | 7:-25208422 | MsaT035969.1:CDS |
| TCATCTATATGTGGAATATG+CGG | 0.642794 | 7:+25208179 | None:intergenic |
| GCAACACCAATGAGAACAAG+GGG | 0.642885 | 7:-25208706 | MsaT035969.1:CDS |
| TGCAACACCAATGAGAACAA+GGG | 0.650590 | 7:-25208707 | MsaT035969.1:CDS |
| GAACAATGTGAACAAGCGAA+AGG | 0.655169 | 7:-25208630 | MsaT035969.1:CDS |
| ACAACCCATATGGAAAGGAG+TGG | 0.680303 | 7:+25208286 | None:intergenic |
| ATGGGATTCTTCCATCACGA+TGG | 0.687731 | 7:+25208607 | None:intergenic |
| TGATGGCCACCTAATGCACG+TGG | 0.714086 | 7:+25208416 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 25208064 | 25208732 | 25208064 | ID=MsaG035969 |
| Chr7 | mRNA | 25208064 | 25208732 | 25208064 | ID=MsaT035969.1;Parent=MsaG035969 |
| Chr7 | exon | 25208064 | 25208732 | 25208064 | ID=MsaT035969.1.exon1;Parent=MsaT035969.1 |
| Chr7 | CDS | 25208064 | 25208732 | 25208064 | ID=cds.MsaT035969.1;Parent=MsaT035969.1 |
| Gene Sequence |
| Protein sequence |
