AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036043


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036043 MtrunA17_Chr4g0008701 80.688 523 92 3 1 518 1 519 0.0 794
MsaG036043 MtrunA17_Chr7g0267601 79.962 524 78 7 1 518 1 503 0.0 777
MsaG036043 MtrunA17_Chr3g0128721 43.836 584 248 10 1 518 1 570 3.17e-127 383
MsaG036043 MtrunA17_Chr8g0361741 51.673 269 127 1 13 281 13 278 2.31e-69 224
MsaG036043 MtrunA17_Chr5g0432691 32.692 156 104 1 7 161 4 159 2.96e-20 87.8
MsaG036043 MtrunA17_Chr3g0107381 32.121 165 107 3 1 163 1 162 5.06e-20 89.0
MsaG036043 MtrunA17_Chr3g0107331 30.968 155 106 1 6 159 2 156 7.47e-19 84.0
MsaG036043 MtrunA17_Chr8g0351751 35.115 131 82 2 4 131 1 131 1.15e-17 82.8
MsaG036043 MtrunA17_Chr4g0014321 31.410 156 95 3 6 154 3 153 3.38e-17 80.9
MsaG036043 MtrunA17_Chr4g0013041 28.780 205 133 5 7 205 4 201 1.09e-16 79.0
MsaG036043 MtrunA17_Chr3g0107351 30.065 153 105 2 6 156 2 154 1.93e-16 77.0
MsaG036043 MtrunA17_Chr3g0107361 30.719 153 104 2 6 156 2 154 1.94e-16 77.0
MsaG036043 MtrunA17_Chr1g0197321 33.043 115 75 2 6 119 3 116 9.13e-16 74.7
MsaG036043 MtrunA17_Chr3g0107341 29.412 153 106 2 6 156 2 154 1.07e-15 74.7
MsaG036043 MtrunA17_Chr2g0283941 28.387 155 109 2 7 159 4 158 2.78e-15 75.5
MsaG036043 MtrunA17_Chr4g0014031 35.897 156 95 3 1 154 1 153 5.76e-15 74.3
MsaG036043 MtrunA17_Chr3g0110131 30.769 156 107 1 6 160 3 158 2.12e-14 73.2
MsaG036043 MtrunA17_Chr5g0420351 34.167 120 74 3 1 119 1 116 5.23e-13 67.0
MsaG036043 MtrunA17_Chr1g0188681 27.607 163 107 4 6 166 3 156 1.41e-12 65.9
MsaG036043 MtrunA17_Chr1g0197261 32.500 120 76 3 1 119 1 116 3.26e-12 64.7
MsaG036043 MtrunA17_Chr2g0296831 34.400 125 78 2 1 124 1 122 3.26e-12 63.5
MsaG036043 MtrunA17_Chr4g0014121 36.458 96 60 1 37 131 62 157 8.13e-12 64.3
MsaG036043 MtrunA17_Chr1g0197461 26.207 145 105 2 6 149 2 145 1.28e-11 62.8
MsaG036043 MtrunA17_Chr4g0014131 27.922 154 95 4 37 174 34 187 2.32e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036043 AT2G40210.1 28.743 167 115 3 4 169 1 164 6.74e-17 82.8
MsaG036043 AT1G65300.1 33.913 115 75 1 6 119 2 116 1.15e-15 77.8
MsaG036043 AT5G06500.1 30.000 150 103 2 6 154 3 151 1.84e-14 73.6
MsaG036043 AT3G05860.3 29.801 151 100 4 1 149 1 147 8.42e-14 71.6
MsaG036043 AT3G05860.1 29.801 151 100 4 1 149 1 147 9.87e-14 71.6
MsaG036043 AT5G26650.1 30.075 133 90 3 7 138 2 132 1.77e-13 72.4
MsaG036043 AT1G31640.1 32.174 115 76 2 6 119 2 115 2.61e-13 72.4
MsaG036043 AT1G31630.1 32.174 115 76 2 6 119 2 115 5.58e-13 70.5
MsaG036043 AT3G05860.2 29.801 151 100 4 1 149 1 147 5.68e-13 68.6
MsaG036043 AT5G27960.1 28.571 133 92 3 7 138 2 132 3.55e-12 68.2
MsaG036043 AT2G28700.1 28.293 205 131 8 4 202 1 195 8.34e-12 67.0
MsaG036043 AT5G48670.1 34.454 119 77 1 4 121 1 119 3.55e-11 65.1
MsaG036043 AT1G65330.1 30.973 113 77 1 8 119 4 116 5.58e-11 63.9
MsaG036043 AT2G15660.1 30.636 173 98 8 5 170 3 160 6.16e-11 63.2

Find 103 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTTCTCAAGGAAATA+TGG 0.173617 7:+26458977 MsaT036043.1:CDS
TTGCCTCCTCAAGGATATTT+TGG 0.199048 7:+26459062 MsaT036043.1:CDS
AGTATTATTCTCAATCAATT+TGG 0.203967 7:-26458112 None:intergenic
TTGGCTAATCAAGCCGATTT+TGG 0.211096 7:+26458795 MsaT036043.1:CDS
CAAAATCTTCAAAGTTAAAT+TGG 0.246957 7:-26459307 None:intergenic
TTGACTCAAACCTGGTTATT+AGG 0.247950 7:-26458284 None:intergenic
GATTGTCACCTGCTCCATTT+TGG 0.265823 7:-26458178 None:intergenic
GGGCATTTCAGTAGCAATAT+TGG 0.280920 7:+26458999 MsaT036043.1:CDS
GAAAGGGTTGTTCACCAAAA+TGG 0.296295 7:+26458164 MsaT036043.1:CDS
AGAAAATGAAGAGACTTTAA+TGG 0.303503 7:+26458404 MsaT036043.1:CDS
CCAACAACCCTTGTTAGATT+TGG 0.312296 7:+26458671 MsaT036043.1:CDS
ATGACAACATAGATAACATT+TGG 0.344877 7:+26459225 MsaT036043.1:CDS
AAGGCATGTGCGGTTGTGTT+TGG 0.348949 7:+26458210 MsaT036043.1:CDS
TTTCTTTCTCAAGGAAATAT+GGG 0.363502 7:+26458978 MsaT036043.1:CDS
AATGGTGATGGGGTACATTC+TGG 0.370012 7:+26458621 MsaT036043.1:CDS
GGTGACAATCTTATGCATTA+AGG 0.370450 7:+26458191 MsaT036043.1:CDS
GGCATTTCAGTAGCAATATT+GGG 0.382881 7:+26459000 MsaT036043.1:CDS
GTGATACAAAACCATCAATA+TGG 0.384286 7:+26458238 MsaT036043.1:CDS
TTTGAAGATCAAGAAAACTT+TGG 0.393915 7:+26459194 MsaT036043.1:CDS
AGGCATGTGCGGTTGTGTTT+GGG 0.393959 7:+26458211 MsaT036043.1:CDS
TTTGAAGTTCAGGGGAACTT+TGG 0.396318 7:+26459416 MsaT036043.1:CDS
GTTATCAAAATCGCCAAAAT+CGG 0.397560 7:-26458808 None:intergenic
CAGGTTTGAGTCAATGTCAT+TGG 0.398460 7:+26458293 MsaT036043.1:CDS
AACTTTGGCAGCAATATTGA+TGG 0.399013 7:+26459533 MsaT036043.1:CDS
AATTATTCCGAAGGAAATCC+AGG 0.399449 7:-26459617 None:intergenic
CTTCAACCCTGGATTTCCTT+CGG 0.403734 7:+26459610 MsaT036043.1:CDS
GTAGGAGATGGCCATATTGA+TGG 0.410732 7:-26458249 None:intergenic
CTACCACATGCAAATTTGAA+TGG 0.411798 7:+26459140 MsaT036043.1:CDS
ATGGTGATGGGGTACATTCT+GGG 0.421537 7:+26458622 MsaT036043.1:CDS
GGCAGCAATATTGATGGAGA+TGG 0.423343 7:+26459539 MsaT036043.1:CDS
GAAAATGAAGAGACTTTAAT+GGG 0.430958 7:+26458405 MsaT036043.1:CDS
TTGTCTAAAGTAAAAGATAT+TGG 0.434434 7:-26458600 None:intergenic
CATTCTGGGGTTAACATGTA+TGG 0.436691 7:+26458636 MsaT036043.1:CDS
GGAGGTCAAAGCAACTTTCA+AGG 0.462180 7:+26459083 MsaT036043.1:CDS
CATGATATGTTTCTTTCTCA+AGG 0.468470 7:+26458969 MsaT036043.1:CDS
ATATCAACACCACCATTGCT+TGG 0.469029 7:-26459491 None:intergenic
TACCACATGCAAATTTGAAT+GGG 0.473760 7:+26459141 MsaT036043.1:CDS
TTTACTTTAGACAATGGTGA+TGG 0.480639 7:+26458609 MsaT036043.1:CDS
GAAGATCAAGAAAACTTTGG+AGG 0.480766 7:+26459197 MsaT036043.1:CDS
TACAAGAGCAACTTCAACCC+TGG 0.483870 7:+26459599 MsaT036043.1:CDS
GAGGTCTAGGCATTTAGAAA+AGG 0.487608 7:+26458539 MsaT036043.1:CDS
CCAAATCTAACAAGGGTTGT+TGG 0.489048 7:-26458671 None:intergenic
CCTACACCTCCAGATCTTAG+TGG 0.490566 7:+26458756 MsaT036043.1:CDS
GAAGTTCAGGGGAACTTTGG+AGG 0.495790 7:+26459419 MsaT036043.1:CDS
ATCAATATGGCCATCTCCTA+CGG 0.501169 7:+26458251 MsaT036043.1:CDS
GATGGAGGAGGATTATTAAG+AGG 0.505755 7:-26458570 None:intergenic
ATGGTTGTTATTGAAACCAC+TGG 0.506528 7:-26458715 None:intergenic
AGATCACTAGAAAGAGGTCT+AGG 0.508379 7:+26458526 MsaT036043.1:CDS
CAATCACCACCACTAAGATC+TGG 0.508447 7:-26458765 None:intergenic
CGCTATAAGAAAAGATTGAA+AGG 0.508733 7:+26458147 MsaT036043.1:CDS
CAACATGCAAATTTGAGTGC+TGG 0.511397 7:+26459251 MsaT036043.1:CDS
TTACTTTAGACAATGGTGAT+GGG 0.517853 7:+26458610 MsaT036043.1:CDS
TGAGAATAATACTCAAAGAA+AGG 0.521008 7:+26458122 MsaT036043.1:CDS
CCACTAAGATCTGGAGGTGT+AGG 0.531744 7:-26458756 None:intergenic
AACAACCATGGAAGTAGCAT+GGG 0.534265 7:+26458729 MsaT036043.1:CDS
GCATCCCCATGCTACTTCCA+TGG 0.535942 7:-26458734 None:intergenic
GTTGTTCACCAAAATGGAGC+AGG 0.556851 7:+26458170 MsaT036043.1:CDS
TCTTCAGCCACCGTAGGAGA+TGG 0.560949 7:-26458261 None:intergenic
AAATTAAGATCACTAGAAAG+AGG 0.562753 7:+26458520 MsaT036043.1:CDS
TGGTGGTGATTGTGATAACT+TGG 0.565716 7:+26458776 MsaT036043.1:CDS
TTTCTCGACCAAATCTAACA+AGG 0.571412 7:-26458679 None:intergenic
AATATGGCCATCTCCTACGG+TGG 0.571701 7:+26458254 MsaT036043.1:CDS
TACTTTAGACAATGGTGATG+GGG 0.572598 7:+26458611 MsaT036043.1:CDS
TAATAATCCTCCTCCATCGT+TGG 0.573169 7:+26458575 MsaT036043.1:CDS
ATTGGTGCCAACGATGGAGG+AGG 0.576273 7:-26458582 None:intergenic
CAATGACATTGACTCAAACC+TGG 0.576547 7:-26458292 None:intergenic
TAACAACCATGGAAGTAGCA+TGG 0.577578 7:+26458728 MsaT036043.1:CDS
AAAGATATTGGTGCCAACGA+TGG 0.578886 7:-26458588 None:intergenic
GTAGGTGTGTTGCCTCCTCA+AGG 0.582180 7:+26459053 MsaT036043.1:CDS
GCTATAAGAAAAGATTGAAA+GGG 0.593678 7:+26458148 MsaT036043.1:CDS
TGGTGATGGGGTACATTCTG+GGG 0.594600 7:+26458623 MsaT036043.1:CDS
GATATTGGTGCCAACGATGG+AGG 0.597015 7:-26458585 None:intergenic
GCAGCAATATTGATGGAGAT+GGG 0.598875 7:+26459540 MsaT036043.1:CDS
TTCTTTCTCAAGGAAATATG+GGG 0.600624 7:+26458979 MsaT036043.1:CDS
ACATTATTGGTAGCAACGCA+AGG 0.601393 7:+26459114 MsaT036043.1:CDS
CCAACACTAGGATTTGTATG+TGG 0.605205 7:-26459362 None:intergenic
TTCTCGACCAAATCTAACAA+GGG 0.605573 7:-26458678 None:intergenic
CATGAAAATCCAAGCAATGG+TGG 0.610991 7:+26459482 MsaT036043.1:CDS
CCACATGAAAATCCAAGCAA+TGG 0.614633 7:+26459479 MsaT036043.1:CDS
TCACCTGAAAATTCTAGTGG+TGG 0.615267 7:+26458858 MsaT036043.1:CDS
CCACATACAAATCCTAGTGT+TGG 0.620141 7:+26459362 MsaT036043.1:CDS
ATTAGGTCTTCAGCCACCGT+AGG 0.620414 7:-26458267 None:intergenic
TTCCCATTCAAATTTGCATG+TGG 0.620497 7:-26459143 None:intergenic
ATTATTCCGAAGGAAATCCA+GGG 0.624169 7:-26459616 None:intergenic
CCATCACCTGAAAATTCTAG+TGG 0.626301 7:+26458855 MsaT036043.1:CDS
GAAAATCCTAGTGGTGGTGT+AGG 0.627650 7:+26459035 MsaT036043.1:CDS
CTTATGCATTAAGGCATGTG+CGG 0.631995 7:+26458200 MsaT036043.1:CDS
TCATCACATGAAAATCATAG+TGG 0.635133 7:+26458939 MsaT036043.1:CDS
ATGCATCAAATTGAAAACGA+AGG 0.641960 7:+26458435 MsaT036043.1:CDS
ACACTAGGATTTGTATGTGG+TGG 0.642124 7:-26459359 None:intergenic
AAAGTTGATCAAATGACCAG+TGG 0.646800 7:+26458699 MsaT036043.1:CDS
AGTGGTTTCAATAACAACCA+TGG 0.647385 7:+26458717 MsaT036043.1:CDS
AACACACCTACACCACCACT+AGG 0.650603 7:-26459041 None:intergenic
CCTCCAAAATATCCTTGAGG+AGG 0.656666 7:-26459065 None:intergenic
ATGATACATGTAGCAACATG+TGG 0.657144 7:+26459450 MsaT036043.1:CDS
TGACCTCCAAAATATCCTTG+AGG 0.660910 7:-26459068 None:intergenic
TGGCTGAAGACCTAATAACC+AGG 0.668231 7:+26458274 MsaT036043.1:CDS
TCACCACCACTAAGATCTGG+AGG 0.674015 7:-26458762 None:intergenic
TCACATGAAAATCCTAGTGG+TGG 0.674534 7:+26459029 MsaT036043.1:CDS
ACAGCAACATTACCAACACT+AGG 0.677687 7:-26459374 None:intergenic
CAATCACATGAAAATCCTAG+TGG 0.684612 7:+26459026 MsaT036043.1:CDS
ACACCTCCAGATCTTAGTGG+TGG 0.688719 7:+26458759 MsaT036043.1:CDS
ACAACCATGGAAGTAGCATG+GGG 0.707731 7:+26458730 MsaT036043.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 26458081 26459637 26458081 ID=MsaG036043
Chr7 mRNA 26458081 26459637 26458081 ID=MsaT036043.1;Parent=MsaG036043
Chr7 exon 26458081 26459637 26458081 ID=MsaT036043.1.exon1;Parent=MsaT036043.1
Chr7 CDS 26458081 26459637 26458081 ID=cds.MsaT036043.1;Parent=MsaT036043.1
Gene Sequence

>MsaT036043.1

ATGACTAGAATCAGAAAAAAGAAGATCACATCCAAATTGATTGAGAATAATACTCAAAGAAAGGCGCGCTATAAGAAAAGATTGAAAGGGTTGTTCACCAAAATGGAGCAGGTGACAATCTTATGCATTAAGGCATGTGCGGTTGTGTTTGGGCTGTGTGATACAAAACCATCAATATGGCCATCTCCTACGGTGGCTGAAGACCTAATAACCAGGTTTGAGTCAATGTCATTGGATGTACAATCAAAAAATAAGACTGACCAATTGACTTTTTTGAAGGATAAGGGCAAAAAACTAGAAGCAAGCCTTGCAAAGATCGAAAAAGAAAATGAAGAGACTTTAATGGGAGCTTATATGCATCAAATTGAAAACGAAGGTAAGTCTTTAGATGATTTTGATCATGATGTGCGTAATCGCTTGATTGCATTTATTCTTGAAAAAATTAAGATCACTAGAAAGAGGTCTAGGCATTTAGAAAAGGAGATCTTGCCTCTTAATAATCCTCCTCCATCGTTGGCACCAATATCTTTTACTTTAGACAATGGTGATGGGGTACATTCTGGGGTTAACATGTATGGACATGTTTTGAACCAACAACCCTTGTTAGATTTGGTCGAGAAAGTTGATCAAATGACCAGTGGTTTCAATAACAACCATGGAAGTAGCATGGGGATGCCTACACCTCCAGATCTTAGTGGTGGTGATTGTGATAACTTGGCTAATCAAGCCGATTTTGGCGATTTTGATAACAACTTTTATAACGGCATGGGAATACCATCACCTGAAAATTCTAGTGGTGGTTTTGATGCTGCGTTTTTTCAAGATAATTTGGAAGATTTTGATGGTGTCATGGAGATATCATCACATGAAAATCATAGTGGTAGTGTTCATGATATGTTTCTTTCTCAAGGAAATATGGGGCATTTCAGTAGCAATATTGGGATACAATCACATGAAAATCCTAGTGGTGGTGTAGGTGTGTTGCCTCCTCAAGGATATTTTGGAGGTCAAAGCAACTTTCAAGGTTTTGATGACATTATTGGTAGCAACGCAAGGATACTACCACATGCAAATTTGAATGGGAATGTTGATTTGTTGCCTCCTCAAGAAAATTTTGAAGATCAAGAAAACTTTGGAGGTTTTGATGACAACATAGATAACATTTGGATACAACATGCAAATTTGAGTGCTGGTAATACATTGCTTCCTCAAGAAAAATTTGGAGACCAATTTAACTTTGAAGATTTTGATAACAATGCAATTAGCAACATGTTGATACCACCACATACAAATCCTAGTGTTGGTAATGTTGCTGTTTTGTCTCATCAAGGAAATTTTGAAGTTCAGGGGAACTTTGGAGGTTTTGATGATGATACATGTAGCAACATGTGGAAACCTCCACATGAAAATCCAAGCAATGGTGGTGTTGATATGATGAATTTTCATCAAAACAACTTTGGCAGCAATATTGATGGAGATGGGATGCAGAGTTTTAATGCGAACTTTGTTGACAATAATTACAAGAGCAACTTCAACCCTGGATTTCCTTCGGAATAA

Protein sequence

>MsaT036043.1

MTRIRKKKITSKLIENNTQRKARYKKRLKGLFTKMEQVTILCIKACAVVFGLCDTKPSIWPSPTVAEDLITRFESMSLDVQSKNKTDQLTFLKDKGKKLEASLAKIEKENEETLMGAYMHQIENEGKSLDDFDHDVRNRLIAFILEKIKITRKRSRHLEKEILPLNNPPPSLAPISFTLDNGDGVHSGVNMYGHVLNQQPLLDLVEKVDQMTSGFNNNHGSSMGMPTPPDLSGGDCDNLANQADFGDFDNNFYNGMGIPSPENSSGGFDAAFFQDNLEDFDGVMEISSHENHSGSVHDMFLSQGNMGHFSSNIGIQSHENPSGGVGVLPPQGYFGGQSNFQGFDDIIGSNARILPHANLNGNVDLLPPQENFEDQENFGGFDDNIDNIWIQHANLSAGNTLLPQEKFGDQFNFEDFDNNAISNMLIPPHTNPSVGNVAVLSHQGNFEVQGNFGGFDDDTCSNMWKPPHENPSNGGVDMMNFHQNNFGSNIDGDGMQSFNANFVDNNYKSNFNPGFPSE*