AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036087


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036087 MtrunA17_Chr7g0267601 83.969 524 63 4 1 524 1 503 0.0 834
MsaG036087 MtrunA17_Chr4g0008701 83.588 524 81 2 1 524 1 519 0.0 829
MsaG036087 MtrunA17_Chr3g0128721 44.330 582 254 12 1 524 1 570 2.96e-133 398
MsaG036087 MtrunA17_Chr8g0361741 53.516 256 115 1 13 268 13 264 7.54e-71 228
MsaG036087 MtrunA17_Chr3g0107381 33.333 165 106 2 1 164 1 162 2.82e-24 101
MsaG036087 MtrunA17_Chr5g0432691 32.051 156 106 0 7 162 4 159 1.30e-22 94.7
MsaG036087 MtrunA17_Chr8g0351751 37.405 131 80 1 4 132 1 131 4.54e-22 95.5
MsaG036087 MtrunA17_Chr3g0107331 34.000 150 98 1 6 154 2 151 1.03e-21 92.0
MsaG036087 MtrunA17_Chr4g0014321 35.246 122 79 0 6 127 3 124 2.93e-21 92.8
MsaG036087 MtrunA17_Chr3g0107351 31.373 153 104 1 6 157 2 154 1.63e-20 88.6
MsaG036087 MtrunA17_Chr3g0107361 32.026 153 103 1 6 157 2 154 1.88e-20 88.6
MsaG036087 MtrunA17_Chr4g0013041 37.302 126 77 1 7 132 4 127 3.18e-20 89.0
MsaG036087 MtrunA17_Chr3g0107341 30.719 153 105 1 6 157 2 154 1.04e-19 86.3
MsaG036087 MtrunA17_Chr4g0014031 37.805 164 89 3 1 160 1 155 3.00e-19 87.0
MsaG036087 MtrunA17_Chr2g0283941 30.345 145 100 1 7 150 4 148 8.69e-19 85.9
MsaG036087 MtrunA17_Chr3g0110131 38.462 117 72 0 6 122 3 119 3.36e-18 84.3
MsaG036087 MtrunA17_Chr1g0197321 32.174 115 77 1 6 120 3 116 9.22e-18 80.5
MsaG036087 MtrunA17_Chr5g0420351 35.833 120 73 2 1 120 1 116 5.13e-17 78.6
MsaG036087 MtrunA17_Chr2g0296831 36.800 125 76 1 1 125 1 122 8.66e-17 76.6
MsaG036087 MtrunA17_Chr4g0014121 39.583 96 58 0 37 132 62 157 2.02e-16 77.8
MsaG036087 MtrunA17_Chr4g0014131 31.875 160 82 4 37 175 34 187 2.20e-16 78.6
MsaG036087 MtrunA17_Chr1g0197461 27.586 145 104 1 6 150 2 145 8.96e-16 74.7
MsaG036087 MtrunA17_Chr1g0188681 33.043 115 76 1 6 120 3 116 2.34e-15 73.6
MsaG036087 MtrunA17_Chr1g0197261 30.000 150 101 2 1 150 1 146 3.24e-15 73.2
MsaG036087 MtrunA17_Chr4g0012761 34.694 147 92 2 6 149 3 148 6.50e-14 69.7
MsaG036087 MtrunA17_Chr1g0193311 37.815 119 69 2 6 120 3 120 1.09e-13 68.9
MsaG036087 MtrunA17_Chr7g0218151 32.653 147 95 2 6 149 3 148 4.59e-13 67.0
MsaG036087 MtrunA17_Chr1g0188661 31.973 147 96 2 6 149 3 148 4.68e-13 67.0
MsaG036087 MtrunA17_Chr1g0188651 28.834 163 104 3 6 167 3 154 8.53e-13 66.2
MsaG036087 MtrunA17_Chr3g0107461 24.359 156 114 2 6 157 3 158 3.64e-12 64.7
MsaG036087 MtrunA17_Chr3g0106501 29.545 88 62 0 35 122 4 91 3.48e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036087 AT2G40210.1 27.933 179 124 3 4 182 1 174 4.36e-20 92.4
MsaG036087 AT1G65300.1 36.522 115 73 0 6 120 2 116 5.75e-20 90.5
MsaG036087 AT3G05860.3 31.387 137 90 2 1 136 1 134 7.56e-17 80.9
MsaG036087 AT3G05860.1 31.387 137 90 2 1 136 1 134 8.04e-17 80.9
MsaG036087 AT3G05860.2 31.387 137 90 2 1 136 1 134 2.03e-16 78.6
MsaG036087 AT5G26650.1 31.579 114 78 0 7 120 2 115 3.86e-16 80.5
MsaG036087 AT5G27960.1 31.579 114 78 0 7 120 2 115 1.34e-15 78.2
MsaG036087 AT1G31630.1 32.174 115 77 1 6 120 2 115 3.46e-15 77.4
MsaG036087 AT5G06500.1 29.167 144 101 1 6 149 3 145 5.55e-15 75.1
MsaG036087 AT1G65330.1 32.743 113 76 0 8 120 4 116 5.92e-15 75.9
MsaG036087 AT1G22590.2 28.440 109 75 1 6 114 3 108 1.12e-14 72.4
MsaG036087 AT2G15660.1 30.058 173 100 7 5 171 3 160 1.51e-14 73.9
MsaG036087 AT1G31640.1 31.304 115 78 1 6 120 2 115 1.64e-14 76.3
MsaG036087 AT5G48670.1 35.897 117 75 0 6 122 3 119 1.94e-14 74.7
MsaG036087 AT2G28700.1 29.915 117 82 0 4 120 1 117 2.24e-14 74.7
MsaG036087 AT5G26630.1 32.212 208 121 6 1 199 1 197 7.99e-14 71.2

Find 104 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTTCCTCAAGAAAATTT+TGG 0.166377 7:-27180884 MsaT036087.1:CDS
TTGACTCATACTTGGTTATT+AGG 0.193064 7:+27181896 None:intergenic
TTGCCTCCTCAAGGATATTT+TGG 0.199048 7:-27181106 MsaT036087.1:CDS
AGTATTATTCTCAATCAATT+TGG 0.203967 7:+27182071 None:intergenic
AACTTTGGCGGCAATATTAA+TGG 0.210065 7:-27180632 MsaT036087.1:CDS
TTGGCTAATCAAGCCGATTT+TGG 0.211096 7:-27181385 MsaT036087.1:CDS
AACAATATAGGTAGCAATAT+TGG 0.211965 7:-27181169 MsaT036087.1:CDS
CAAAATCTTCAAAGTTAAAT+TGG 0.246957 7:+27180858 None:intergenic
GATTGTCACCTGCTCCATTT+TGG 0.265823 7:+27182005 None:intergenic
GAAAGGGTTGTTCACCAAAA+TGG 0.296295 7:-27182019 MsaT036087.1:CDS
GATGGTGATGGGGTACATTC+TGG 0.296453 7:-27181559 MsaT036087.1:CDS
CCAACAACCCTTGTTAGATT+TGG 0.298734 7:-27181509 MsaT036087.1:CDS
AGAAAATGAAGAGACTTTAA+TGG 0.303503 7:-27181776 MsaT036087.1:CDS
GAGGCATGTGCGGTTGTGTT+TGG 0.344211 7:-27181970 MsaT036087.1:CDS
GTTATCAAAATTGCCAAAAT+CGG 0.349719 7:+27181372 None:intergenic
TTTGAAGTTCACAGAAACTT+TGG 0.364250 7:-27180749 MsaT036087.1:CDS
TGTTTCTTCCTCATGGAAAT+CGG 0.364437 7:-27181204 MsaT036087.1:CDS
ACTTTGTTGACAATAATTAC+AGG 0.379077 7:-27180583 MsaT036087.1:CDS
ACAATATAGGTAGCAATATT+GGG 0.381596 7:-27181168 MsaT036087.1:CDS
AGGCATGTGCGGTTGTGTTT+GGG 0.389221 7:-27181969 MsaT036087.1:CDS
GTGATACAAAACCATCAATA+TGG 0.392745 7:-27181942 MsaT036087.1:CDS
TTTGATGATCAAGAAAACTT+TGG 0.393915 7:-27180974 MsaT036087.1:CDS
TCTTCTAAAGTAAAAGATAT+TGG 0.398866 7:+27181580 None:intergenic
GTAGGAGATGGCCATATTGA+TGG 0.410732 7:+27181931 None:intergenic
GTTTCTTCCTCATGGAAATC+GGG 0.417845 7:-27181203 MsaT036087.1:CDS
CAATGACATTGACTCATACT+TGG 0.420310 7:+27181888 None:intergenic
CATGATATGTTTCTTCCTCA+TGG 0.427152 7:-27181211 MsaT036087.1:CDS
GAAAATGAAGAGACTTTAAT+GGG 0.430958 7:-27181775 MsaT036087.1:CDS
CAAGTATGAGTCAATGTCAT+TGG 0.431413 7:-27181887 MsaT036087.1:CDS
ATGGTGATGGGGTACATTCT+GGG 0.432610 7:-27181558 MsaT036087.1:CDS
CATTCTGGGGTTAACATGTA+TGG 0.436691 7:-27181544 MsaT036087.1:CDS
GGCGGCAATATTAATGGAGA+TGG 0.442349 7:-27180626 MsaT036087.1:CDS
TGTGCGGTTGTGTTTGGGCC+AGG 0.446264 7:-27181964 MsaT036087.1:CDS
CCAAATCTAACAAGGGTTGT+TGG 0.460158 7:+27181509 None:intergenic
GGAGGTCAAAGCAACTTTCA+AGG 0.462180 7:-27181085 MsaT036087.1:CDS
GATGATCAAGAAAACTTTGG+AGG 0.463709 7:-27180971 MsaT036087.1:CDS
ATATCAACACCACCATTGCT+TGG 0.469029 7:+27180674 None:intergenic
TACAGGAACAACTTCAACCC+TGG 0.480219 7:-27180566 MsaT036087.1:CDS
TTTCATCAAAACAACTTTGG+CGG 0.485753 7:-27180644 MsaT036087.1:CDS
GTTGGAGGAGGATTATTAAG+AGG 0.490332 7:+27181610 None:intergenic
GATGTCTAGGCATTTAGAAA+AGG 0.494516 7:-27181641 MsaT036087.1:CDS
ATCAATATGGCCATCTCCTA+CGG 0.501169 7:-27181929 MsaT036087.1:CDS
TTTACTTTAGAAGATGGTGA+TGG 0.506036 7:-27181571 MsaT036087.1:CDS
ATGGTTGTTATTGAAACCAC+TGG 0.506528 7:+27181465 None:intergenic
TTACTTTAGAAGATGGTGAT+GGG 0.515841 7:-27181570 MsaT036087.1:CDS
GATATTGGTGCCAACGTTGG+AGG 0.517309 7:+27181595 None:intergenic
TGAGAATAATACTCAAAGAA+AGG 0.521008 7:-27182061 MsaT036087.1:CDS
ATACCACCACATACAAATCA+TGG 0.521732 7:-27180809 MsaT036087.1:CDS
GACAATCTTATGTGGAATTG+AGG 0.522788 7:-27181989 MsaT036087.1:CDS
GTATCTCCATGCTACTTCCA+TGG 0.531474 7:+27181446 None:intergenic
CCTCCACTAACAGATCTTAG+TGG 0.531606 7:-27181424 MsaT036087.1:CDS
ATTGGTGCCAACGTTGGAGG+AGG 0.533160 7:+27181598 None:intergenic
CTAACAGATCTTAGTGGTGG+TGG 0.538898 7:-27181418 MsaT036087.1:CDS
GTTATAAGAAAAGATTGAAA+GGG 0.540577 7:-27182035 MsaT036087.1:CDS
AACAACTTTGATGGTGGCAT+GGG 0.547295 7:-27181352 MsaT036087.1:CDS
ATATTAATGGAGATGGGATG+CGG 0.549478 7:-27180619 MsaT036087.1:CDS
CGTTATAAGAAAAGATTGAA+AGG 0.551031 7:-27182036 MsaT036087.1:CDS
GAAGTTCACAGAAACTTTGG+AGG 0.552988 7:-27180746 MsaT036087.1:CDS
GCGGCAATATTAATGGAGAT+GGG 0.556348 7:-27180625 MsaT036087.1:CDS
GTTGTTCACCAAAATGGAGC+AGG 0.556851 7:-27182013 MsaT036087.1:CDS
TTTGATAACAACTTTGATGG+TGG 0.557716 7:-27181358 MsaT036087.1:CDS
TCTTCAGCCACCGTAGGAGA+TGG 0.560949 7:+27181919 None:intergenic
CCACCACTAAGATCTGTTAG+TGG 0.562274 7:+27181421 None:intergenic
TGGTGGTGGTTGTGATAACT+TGG 0.567708 7:-27181404 MsaT036087.1:CDS
ACATTACAGGTAGCAATACA+AGG 0.567975 7:-27181054 MsaT036087.1:CDS
AGATCACTAGAAAGATGTCT+AGG 0.570407 7:-27181654 MsaT036087.1:CDS
AATATGGCCATCTCCTACGG+TGG 0.571701 7:-27181926 MsaT036087.1:CDS
TTTCTCAACCAAATCTAACA+AGG 0.573944 7:+27181501 None:intergenic
TAATAATCCTCCTCCAACGT+TGG 0.577508 7:-27181605 MsaT036087.1:CDS
AAAGATATTGGTGCCAACGT+TGG 0.577514 7:+27181592 None:intergenic
GTAGGTGTGTTGCCTCCTCA+AGG 0.582180 7:-27181115 MsaT036087.1:CDS
TACTTTAGAAGATGGTGATG+GGG 0.582286 7:-27181569 MsaT036087.1:CDS
CCAACACCATGATTTGTATG+TGG 0.583138 7:+27180803 None:intergenic
GAGCAGGTGACAATCTTATG+TGG 0.583955 7:-27181997 MsaT036087.1:CDS
TAACAACTTTGATGGTGGCA+TGG 0.586911 7:-27181353 MsaT036087.1:CDS
TGGTGATGGGGTACATTCTG+GGG 0.588241 7:-27181557 MsaT036087.1:CDS
TAACAACCATGGAAGTAGCA+TGG 0.590557 7:-27181452 MsaT036087.1:CDS
TTTATTCTGAAGGAAATCCA+GGG 0.595152 7:+27180549 None:intergenic
TTTCTTCCTCATGGAAATCG+GGG 0.609048 7:-27181202 MsaT036087.1:CDS
TTCTCAACCAAATCTAACAA+GGG 0.610146 7:+27181502 None:intergenic
CATGAAAATCCAAGCAATGG+TGG 0.610991 7:-27180683 MsaT036087.1:CDS
CCACATGAAAATCCAAGCAA+TGG 0.614633 7:-27180686 MsaT036087.1:CDS
TCACCTGAAAATTCTAGTGG+TGG 0.615267 7:-27181322 MsaT036087.1:CDS
CCACATACAAATCATGGTGT+TGG 0.616507 7:-27180803 MsaT036087.1:CDS
ATTAGGTCTTCAGCCACCGT+AGG 0.620414 7:+27181913 None:intergenic
CCATCACCTGAAAATTCTAG+TGG 0.626301 7:-27181325 MsaT036087.1:CDS
GAAAATCCTAGTGGTGGTGT+AGG 0.627650 7:-27181133 MsaT036087.1:CDS
TCATCACATGAAAATCATAG+TGG 0.635133 7:-27181241 MsaT036087.1:CDS
ATGCATCAAATTGAAAACGA+AGG 0.641960 7:-27181745 MsaT036087.1:CDS
AAAGTTGATCAAATGACCAG+TGG 0.646800 7:-27181481 MsaT036087.1:CDS
AGTGGTTTCAATAACAACCA+TGG 0.647385 7:-27181463 MsaT036087.1:CDS
TTCTCATTCAGATTTGCATG+TGG 0.650408 7:+27181025 None:intergenic
AACACACCTACACCACCACT+AGG 0.650603 7:+27181127 None:intergenic
TTCTTCCTCATGGAAATCGG+GGG 0.655717 7:-27181201 MsaT036087.1:CDS
CCTCCAAAATATCCTTGAGG+AGG 0.656666 7:+27181103 None:intergenic
ATGATACATGTAGCAACATG+TGG 0.657144 7:-27180715 MsaT036087.1:CDS
ATGTGGAATTGAGGCATGTG+CGG 0.658684 7:-27181980 MsaT036087.1:CDS
TGACCTCCAAAATATCCTTG+AGG 0.660910 7:+27181100 None:intergenic
CCACTAAGATCTGTTAGTGG+AGG 0.673960 7:+27181424 None:intergenic
TCACATGAAAATCCTAGTGG+TGG 0.674534 7:-27181139 MsaT036087.1:CDS
ACAACATAGATAGCAGCATG+TGG 0.675578 7:-27180940 MsaT036087.1:CDS
CAATCACATGAAAATCCTAG+TGG 0.684612 7:-27181142 MsaT036087.1:CDS
CCACTAACAGATCTTAGTGG+TGG 0.700550 7:-27181421 MsaT036087.1:CDS
ACACCATGATTTGTATGTGG+TGG 0.720749 7:+27180806 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 27180550 27182124 27180550 ID=MsaG036087
Chr7 mRNA 27180550 27182124 27180550 ID=MsaT036087.1;Parent=MsaG036087
Chr7 exon 27180550 27182124 27180550 ID=MsaT036087.1.exon1;Parent=MsaT036087.1
Chr7 CDS 27180550 27182124 27180550 ID=cds.MsaT036087.1;Parent=MsaT036087.1
Gene Sequence

>MsaT036087.1

ATGACTAGAATCAGAAAAAAGAAGATCACATCCAAATTGATTGAGAATAATACTCAAAGAAAGGCGCGTTATAAGAAAAGATTGAAAGGGTTGTTCACCAAAATGGAGCAGGTGACAATCTTATGTGGAATTGAGGCATGTGCGGTTGTGTTTGGGCCAGGTGATACAAAACCATCAATATGGCCATCTCCTACGGTGGCTGAAGACCTAATAACCAAGTATGAGTCAATGTCATTGGATGTACAATCGAAAAATAAGACTGACCAATTGACTTTTTTGAAGGATAAGGGCAAAAAACTTGAAGCAAGCCTTGCAAAGATCGAAAAAGAAAATGAAGAGACTTTAATGGGAGCTTATATGCATCAAATTGAAAACGAAGGTAAGTCTTTAGATGATTTTGATCATGATGTGCTGAATCGTTTGATTGCTTTTACACTTGAAAAAATTAAGATCACTAGAAAGATGTCTAGGCATTTAGAAAAGGAGATCTTGCCTCTTAATAATCCTCCTCCAACGTTGGCACCAATATCTTTTACTTTAGAAGATGGTGATGGGGTACATTCTGGGGTTAACATGTATGGACATGTTTTGAACCAACAACCCTTGTTAGATTTGGTTGAGAAAGTTGATCAAATGACCAGTGGTTTCAATAACAACCATGGAAGTAGCATGGAGATACCTCCACTAACAGATCTTAGTGGTGGTGGTTGTGATAACTTGGCTAATCAAGCCGATTTTGGCAATTTTGATAACAACTTTGATGGTGGCATGGGAATACCATCACCTGAAAATTCTAGTGGTGGTTTTTATGCAGCGTTTTTTCAAGATAATTTGCAAGATTTTGATAGTATCATGGAGATATCATCACATGAAAATCATAGTGGTAGTGTTCATGATATGTTTCTTCCTCATGGAAATCGGGGGCATTTTATTAACAATATAGGTAGCAATATTGGGATACAATCACATGAAAATCCTAGTGGTGGTGTAGGTGTGTTGCCTCCTCAAGGATATTTTGGAGGTCAAAGCAACTTTCAAGGTTTTGATGACATTACAGGTAGCAATACAAGGATACTACCACATGCAAATCTGAATGAGAATGTTGATTTGTTGCCTCCTCAAGAAAATTTTGATGATCAAGAAAACTTTGGAGGTTTTGATGACAACATAGATAGCAGCATGTGGATACAACATGCAAATTTTAGTGGTGGTGATACATTGCTTCCTCAAGAAAATTTTGGAGACCAATTTAACTTTGAAGATTTTGATAACAACACTGGTAGCAACATGTTGATACCACCACATACAAATCATGGTGTTGGTAATGTTGATGTTTTGTCTCATCAAAGAAATTTTGAAGTTCACAGAAACTTTGGAGGTTTTGATGATGATACATGTAGCAACATGTGGAAACCTCCACATGAAAATCCAAGCAATGGTGGTGTTGATATGATGACTTTTCATCAAAACAACTTTGGCGGCAATATTAATGGAGATGGGATGCGGAGTTTTAATGCGAACTTTGTTGACAATAATTACAGGAACAACTTCAACCCTGGATTTCCTTCAGAATAA

Protein sequence

>MsaT036087.1

MTRIRKKKITSKLIENNTQRKARYKKRLKGLFTKMEQVTILCGIEACAVVFGPGDTKPSIWPSPTVAEDLITKYESMSLDVQSKNKTDQLTFLKDKGKKLEASLAKIEKENEETLMGAYMHQIENEGKSLDDFDHDVLNRLIAFTLEKIKITRKMSRHLEKEILPLNNPPPTLAPISFTLEDGDGVHSGVNMYGHVLNQQPLLDLVEKVDQMTSGFNNNHGSSMEIPPLTDLSGGGCDNLANQADFGNFDNNFDGGMGIPSPENSSGGFYAAFFQDNLQDFDSIMEISSHENHSGSVHDMFLPHGNRGHFINNIGSNIGIQSHENPSGGVGVLPPQGYFGGQSNFQGFDDITGSNTRILPHANLNENVDLLPPQENFDDQENFGGFDDNIDSSMWIQHANFSGGDTLLPQENFGDQFNFEDFDNNTGSNMLIPPHTNHGVGNVDVLSHQRNFEVHRNFGGFDDDTCSNMWKPPHENPSNGGVDMMTFHQNNFGGNINGDGMRSFNANFVDNNYRNNFNPGFPSE*