AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036131


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036131 MtrunA17_Chr7g0223351 84.971 173 26 0 1 173 1 173 3.14e-108 306
MsaG036131 MtrunA17_Chr7g0223361 68.208 173 54 1 1 172 1 173 1.64e-85 249
MsaG036131 MtrunA17_Chr7g0273661 59.868 152 59 2 7 156 7 158 5.33e-59 181
MsaG036131 MtrunA17_Chr1g0204951 41.566 166 94 3 7 169 22 187 8.58e-38 128
MsaG036131 MtrunA17_Chr5g0394621 39.881 168 96 3 7 169 20 187 3.96e-36 124
MsaG036131 MtrunA17_Chr1g0204971 39.759 166 97 3 7 169 22 187 1.86e-34 120
MsaG036131 MtrunA17_Chr5g0394631 41.520 171 91 6 7 169 229 398 2.48e-33 122
MsaG036131 MtrunA17_Chr5g0394631 37.654 162 96 3 7 163 20 181 2.52e-28 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036131 AT2G32020.1 39.884 173 100 3 1 171 12 182 6.77e-47 151
MsaG036131 AT2G32030.1 39.759 166 96 3 8 171 24 187 1.02e-42 141
MsaG036131 AT3G22560.1 41.520 171 96 3 3 169 2 172 6.66e-38 128

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTATTGGGTTCAATTAT+TGG 0.148532 7:+27803835 MsaT036131.1:CDS
TGCTATTGGGTTCAATTATT+GGG 0.160306 7:+27803836 MsaT036131.1:CDS
TGCAAAGTTTAAAGCAGATT+TGG 0.219727 7:+27803809 MsaT036131.1:CDS
TTCTGACTTGCAAAGGTTGC+AGG 0.286836 7:+27803917 MsaT036131.1:CDS
GCAAAGTTTAAAGCAGATTT+GGG 0.349624 7:+27803810 MsaT036131.1:CDS
TTAAACTTTGCATAAACAAT+TGG 0.394119 7:-27803798 None:intergenic
TTTGCAAGTCAGAAAACTCT+TGG 0.405968 7:-27803908 None:intergenic
TCAGCATCACTTTCTTTGAA+TGG 0.406038 7:-27803609 None:intergenic
CATCAAGACATATTGATTGT+TGG 0.420749 7:-27803746 None:intergenic
AGACATATTGATTGTTGGAA+TGG 0.429733 7:-27803741 None:intergenic
GACATATTGATTGTTGGAAT+GGG 0.431307 7:-27803740 None:intergenic
TTGACTTACATAACTCCAAT+CGG 0.443073 7:-27803677 None:intergenic
CATCACTTTCTTTGAATGGC+CGG 0.455673 7:-27803605 None:intergenic
TGCTGACGATGTGTTGTTGT+GGG 0.482447 7:+27803626 MsaT036131.1:CDS
GGGTTCAATTATTGGGGCCA+AGG 0.491840 7:+27803843 MsaT036131.1:CDS
GCTATTGGGTTCAATTATTG+GGG 0.503790 7:+27803837 MsaT036131.1:CDS
TGTCTTGATGATCACTCCAT+TGG 0.505623 7:+27803759 MsaT036131.1:CDS
CATGGCTTCACAAAGGCTGT+TGG 0.533558 7:+27803959 MsaT036131.1:CDS
AAAGTTGGCTTTCATAGGGA+GGG 0.561963 7:+27803984 MsaT036131.1:CDS
TGACTTACATAACTCCAATC+GGG 0.564921 7:-27803676 None:intergenic
TAGGGAGGGATTGTTCAGAA+AGG 0.567572 7:+27803998 MsaT036131.1:CDS
ATGCTGACGATGTGTTGTTG+TGG 0.569510 7:+27803625 MsaT036131.1:CDS
ATGAGTCAAGGATCACTCTC+CGG 0.583353 7:+27803586 MsaT036131.1:CDS
AGTCATGCAAAATACCCGAT+TGG 0.592545 7:+27803662 MsaT036131.1:CDS
GATTGGAGTTATGTAAGTCA+AGG 0.598815 7:+27803679 MsaT036131.1:CDS
TGACTCATGATCTATTGCCA+TGG 0.604394 7:-27803571 None:intergenic
AAAAGTTGGCTTTCATAGGG+AGG 0.608005 7:+27803983 MsaT036131.1:CDS
ACTAATCAAACAACATCATG+AGG 0.616668 7:-27804086 None:intergenic
ATTGGAGTTATGTAAGTCAA+GGG 0.627205 7:+27803680 MsaT036131.1:CDS
TGGCAATAGATCATGAGTCA+AGG 0.642231 7:+27803574 MsaT036131.1:CDS
TAATATTCTTGTAGACAACA+TGG 0.652110 7:+27803941 MsaT036131.1:CDS
TAGACAACATGGCTTCACAA+AGG 0.690615 7:+27803952 MsaT036131.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 27803573 27804103 27803573 ID=MsaG036131
Chr7 mRNA 27803573 27804103 27803573 ID=MsaT036131.1;Parent=MsaG036131
Chr7 exon 27803573 27804103 27803573 ID=MsaT036131.1.exon1;Parent=MsaT036131.1
Chr7 CDS 27803573 27804103 27803573 ID=cds.MsaT036131.1;Parent=MsaT036131.1
Gene Sequence

>MsaT036131.1

ATGGCAATAGATCATGAGTCAAGGATCACTCTCCGGCCATTCAAAGAAAGTGATGCTGACGATGTGTTGTTGTGGGCAAGTGATGAAAGAGTCATGCAAAATACCCGATTGGAGTTATGTAAGTCAAGGGAACAAGCTTTGAATTTCATAAAAAAAGAATGTACTTACCCATTCCAACAATCAATATGTCTTGATGATCACTCCATTGGAATTGTTTTTGTACTTCCAATTGTTTATGCAAAGTTTAAAGCAGATTTGGGTTTTGCTATTGGGTTCAATTATTGGGGCCAAGGTATAGCCACAAAAGCACTTAAGATTCTCTTGTCTCAAGTGTTCCAAGAGTTTTCTGACTTGCAAAGGTTGCAGGCTAATATTCTTGTAGACAACATGGCTTCACAAAGGCTGTTGGAAAAAGTTGGCTTTCATAGGGAGGGATTGTTCAGAAAGGTTTTTCATCAGAAAGGGAATATTGAGAATGCATATATTTTCAGTTTCTTGGCTACTGATGAAATTCCTCATGATGTTGTTTGA

Protein sequence

>MsaT036131.1

MAIDHESRITLRPFKESDADDVLLWASDERVMQNTRLELCKSREQALNFIKKECTYPFQQSICLDDHSIGIVFVLPIVYAKFKADLGFAIGFNYWGQGIATKALKILLSQVFQEFSDLQRLQANILVDNMASQRLLEKVGFHREGLFRKVFHQKGNIENAYIFSFLATDEIPHDVV*