AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036132

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036132 MtrunA17_Chr7g0223351 93.750 176 11 0 1 176 1 176 5.65e-123 343
MsaG036132 MtrunA17_Chr7g0223361 68.786 173 53 1 1 172 1 173 1.01e-83 244
MsaG036132 MtrunA17_Chr7g0273661 57.595 158 65 2 1 156 1 158 2.30e-58 179
MsaG036132 MtrunA17_Chr1g0204951 42.771 166 92 3 7 169 22 187 1.27e-39 133
MsaG036132 MtrunA17_Chr1g0204971 40.964 166 95 3 7 169 22 187 1.02e-36 125
MsaG036132 MtrunA17_Chr5g0394631 40.936 171 92 6 7 169 229 398 2.78e-35 127
MsaG036132 MtrunA17_Chr5g0394631 36.420 162 98 3 7 163 20 181 2.82e-29 111
MsaG036132 MtrunA17_Chr5g0394621 39.286 168 97 3 7 169 20 187 7.59e-35 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036132 AT2G32020.1 40.462 173 99 3 1 171 12 182 3.79e-45 147
MsaG036132 AT2G32030.1 40.361 166 95 3 8 171 24 187 1.38e-42 140
MsaG036132 AT3G22560.1 42.690 171 94 3 3 169 2 172 2.38e-42 140

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAGTTTAAAGCAGATTT+AGG 0.191234 7:+27807242 MsaT036132.1:CDS
TGCTATTGGGTTCAACTATT+GGG 0.215244 7:+27807268 MsaT036132.1:CDS
ATGCTATTGGGTTCAACTAT+TGG 0.242032 7:+27807267 MsaT036132.1:CDS
TCCTGATTTGGTAAGGTTGC+AGG 0.261225 7:+27807349 MsaT036132.1:CDS
GCAGATTTAGGTTATGCTAT+TGG 0.268381 7:+27807254 MsaT036132.1:CDS
ATTCCAAGACTTTCCTGATT+TGG 0.305046 7:+27807337 MsaT036132.1:CDS
CAGATTTAGGTTATGCTATT+GGG 0.314452 7:+27807255 MsaT036132.1:CDS
TGTCTTGATGATCACTCTAT+TGG 0.344658 7:+27807191 MsaT036132.1:CDS
GCCTGCAACCTTACCAAATC+AGG 0.389988 7:-27807350 None:intergenic
TCCTGAACAATCCCTCCCTT+TGG 0.399368 7:-27807427 None:intergenic
TCAGCATCACTTTCTTTGAA+TGG 0.407648 7:-27807041 None:intergenic
CATCAAGACATATTGATTGT+TGG 0.420749 7:-27807178 None:intergenic
TTAAACTTTGCATCAAGATA+AGG 0.441955 7:-27807230 None:intergenic
AGACATATTGATTGTTGGAA+TGG 0.451178 7:-27807173 None:intergenic
TGCTGATGATGTGTTGTTGT+GGG 0.452299 7:+27807058 MsaT036132.1:CDS
TCCAAAGGGAGGGATTGTTC+AGG 0.453041 7:+27807426 MsaT036132.1:CDS
CATCACTTTCTTTGAATGGC+CGG 0.455673 7:-27807037 None:intergenic
GGAAGGGTATTTATAACAAA+GGG 0.457413 7:+27807447 MsaT036132.1:CDS
AAGAATCTTCACTGCTTTCG+TGG 0.476541 7:-27807303 None:intergenic
AAGACTTTCCTGATTTGGTA+AGG 0.487902 7:+27807342 MsaT036132.1:CDS
TTACCAAATCAGGAAAGTCT+TGG 0.502664 7:-27807340 None:intergenic
CTGCTTTCGTGGCTACACCT+TGG 0.503138 7:-27807292 None:intergenic
TGGAAAATGTTGGTTTCCAA+AGG 0.508065 7:+27807411 MsaT036132.1:CDS
CGATGAAATTCCTCATGCTA+AGG 0.513932 7:+27807508 MsaT036132.1:CDS
GGGTTCAACTATTGGGGCCA+AGG 0.514130 7:+27807275 MsaT036132.1:CDS
GCTATTGGGTTCAACTATTG+GGG 0.535162 7:+27807269 MsaT036132.1:CDS
AGGAAGGGTATTTATAACAA+AGG 0.538353 7:+27807446 MsaT036132.1:CDS
ATGCTGATGATGTGTTGTTG+TGG 0.542577 7:+27807057 MsaT036132.1:CDS
GATGAAATTCCTCATGCTAA+GGG 0.544681 7:+27807509 MsaT036132.1:CDS
AGTCATGCAATATATGCGAT+TGG 0.560410 7:+27807094 MsaT036132.1:CDS
GGAAAATGTTGGTTTCCAAA+GGG 0.561564 7:+27807412 MsaT036132.1:CDS
AATGTTGGTTTCCAAAGGGA+GGG 0.578884 7:+27807416 MsaT036132.1:CDS
AAGAGTCAAGGATCACTCTC+CGG 0.583353 7:+27807018 MsaT036132.1:CDS
AAATGTTGGTTTCCAAAGGG+AGG 0.598591 7:+27807415 MsaT036132.1:CDS
ACTAATTAACCCTTAGCATG+AGG 0.612515 7:-27807518 None:intergenic
TTATAACAAAGGGAATGTAG+AGG 0.639782 7:+27807457 MsaT036132.1:CDS
CTTAGCATGAGGAATTTCAT+CGG 0.640114 7:-27807507 None:intergenic
TGGCAATAGATCAAGAGTCA+AGG 0.642231 7:+27807006 MsaT036132.1:CDS
AGGGAGGGATTGTTCAGGAA+GGG 0.644482 7:+27807431 MsaT036132.1:CDS
AAGGGAGGGATTGTTCAGGA+AGG 0.654455 7:+27807430 MsaT036132.1:CDS
GATTGGAGTTATGTAAAACA+CGG 0.661703 7:+27807111 MsaT036132.1:CDS
ATTGGAGTTATGTAAAACAC+GGG 0.678767 7:+27807112 MsaT036132.1:CDS
ACTTTGCATCAAGATAAGGA+AGG 0.680492 7:-27807226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 27807005 27807535 27807005 ID=MsaG036132
Chr7 mRNA 27807005 27807535 27807005 ID=MsaT036132.1;Parent=MsaG036132
Chr7 exon 27807005 27807535 27807005 ID=MsaT036132.1.exon1;Parent=MsaT036132.1
Chr7 CDS 27807005 27807535 27807005 ID=cds.MsaT036132.1;Parent=MsaT036132.1
Gene Sequence

>MsaT036132.1

ATGGCAATAGATCAAGAGTCAAGGATCACTCTCCGGCCATTCAAAGAAAGTGATGCTGATGATGTGTTGTTGTGGGCAAGTGATGAAAGAGTCATGCAATATATGCGATTGGAGTTATGTAAAACACGGGAACAAGCTTTGAATTTCATAAAAAATGAATGCATTTATCCATTCCAACAATCAATATGTCTTGATGATCACTCTATTGGAATTGTTTTTGTCCTTCCTTATCTTGATGCAAAGTTTAAAGCAGATTTAGGTTATGCTATTGGGTTCAACTATTGGGGCCAAGGTGTAGCCACGAAAGCAGTGAAGATTCTTTTGTCTTGTGTATTCCAAGACTTTCCTGATTTGGTAAGGTTGCAGGCTAATACTCGTCCAGACAACATAGCTTCTCAAAAACTGTTGGAAAATGTTGGTTTCCAAAGGGAGGGATTGTTCAGGAAGGGTATTTATAACAAAGGGAATGTAGAGGATTTCTGTATTTTTAGTTTCTTGTCTACCGATGAAATTCCTCATGCTAAGGGTTAA
Protein sequence

>MsaT036132.1

MAIDQESRITLRPFKESDADDVLLWASDERVMQYMRLELCKTREQALNFIKNECIYPFQQSICLDDHSIGIVFVLPYLDAKFKADLGYAIGFNYWGQGVATKAVKILLSCVFQDFPDLVRLQANTRPDNIASQKLLENVGFQREGLFRKGIYNKGNVEDFCIFSFLSTDEIPHAKG*