AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036135

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036135 MtrunA17_Chr7g0223311 91.420 338 22 4 1 335 40 373 0.0 550
MsaG036135 MtrunA17_Chr4g0010151 40.132 304 154 9 9 308 55 334 3.49e-50 171
MsaG036135 MtrunA17_Chr4g0031411 41.600 250 99 10 16 252 55 270 5.64e-41 146
MsaG036135 MtrunA17_Chr2g0328021 41.597 238 69 10 16 235 26 211 1.36e-40 144
MsaG036135 MtrunA17_Chr7g0237921 37.421 318 153 11 13 323 53 331 2.41e-40 144
MsaG036135 MtrunA17_Chr8g0374251 77.778 63 14 0 39 101 69 131 7.48e-32 122
MsaG036135 MtrunA17_Chr8g0367161 57.143 91 29 1 12 102 16 96 7.94e-31 119
MsaG036135 MtrunA17_Chr8g0374111 59.551 89 29 2 40 122 31 118 2.00e-30 115
MsaG036135 MtrunA17_Chr2g0282441 56.311 103 37 2 23 118 11 112 2.44e-29 115
MsaG036135 MtrunA17_Chr8g0341671 66.667 75 24 1 20 93 1 75 2.86e-29 112
MsaG036135 MtrunA17_Chr3g0127201 62.353 85 27 1 8 92 2 81 4.64e-29 113
MsaG036135 MtrunA17_Chr3g0117601 59.091 88 31 2 1 88 29 111 7.72e-29 113
MsaG036135 MtrunA17_Chr5g0411951 88.000 50 6 0 39 88 44 93 8.46e-29 113
MsaG036135 MtrunA17_Chr4g0061761 86.538 52 7 0 37 88 39 90 9.60e-29 113
MsaG036135 MtrunA17_Chr3g0126201 47.414 116 50 2 37 152 50 154 1.49e-28 112
MsaG036135 MtrunA17_Chr4g0030051 83.333 54 9 0 37 90 40 93 3.44e-28 110
MsaG036135 MtrunA17_Chr8g0346551 72.464 69 14 1 40 103 26 94 9.10e-28 109
MsaG036135 MtrunA17_Chr1g0188851 60.274 73 29 0 22 94 27 99 1.63e-27 109
MsaG036135 MtrunA17_Chr2g0326001 78.571 56 12 0 37 92 39 94 3.07e-27 108
MsaG036135 MtrunA17_Chr2g0308011 88.000 50 6 0 37 86 36 85 5.11e-27 105
MsaG036135 MtrunA17_Chr2g0282511 86.000 50 7 0 37 86 44 93 1.01e-26 107
MsaG036135 MtrunA17_Chr1g0177251 79.245 53 11 0 40 92 14 66 2.73e-26 105
MsaG036135 MtrunA17_Chr2g0282051 69.697 66 18 1 30 95 38 101 4.39e-26 105
MsaG036135 MtrunA17_Chr4g0047761 73.770 61 14 1 35 95 69 127 2.99e-25 103
MsaG036135 MtrunA17_Chr6g0453321 76.471 51 12 0 40 90 138 188 8.37e-24 101
MsaG036135 MtrunA17_Chr2g0283811 76.000 50 12 0 40 89 49 98 1.10e-23 95.5
MsaG036135 MtrunA17_Chr4g0042481 56.962 79 23 2 40 118 124 191 4.97e-23 99.4
MsaG036135 MtrunA17_Chr8g0355261 69.811 53 16 0 40 92 120 172 7.85e-23 98.6
MsaG036135 MtrunA17_Chr7g0217581 74.510 51 13 0 40 90 234 284 8.09e-23 99.4
MsaG036135 MtrunA17_Chr3g0092111 67.213 61 16 1 40 100 138 194 1.18e-22 98.2
MsaG036135 MtrunA17_Chr6g0460721 57.534 73 27 1 18 90 73 141 3.24e-22 95.5
MsaG036135 MtrunA17_Chr5g0417571 49.425 87 42 1 4 90 106 190 3.96e-22 95.1
MsaG036135 MtrunA17_Chr7g0254161 67.241 58 18 1 39 96 102 158 5.66e-22 95.9
MsaG036135 MtrunA17_Chr5g0417601 68.966 58 17 1 40 97 142 198 1.06e-21 94.7
MsaG036135 MtrunA17_Chr5g0417551 45.000 120 51 4 40 153 129 239 2.25e-21 93.6
MsaG036135 MtrunA17_Chr6g0460731 67.308 52 17 0 39 90 97 148 2.43e-21 93.6
MsaG036135 MtrunA17_Chr5g0417561 43.548 124 47 4 40 153 121 231 6.38e-21 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036135 AT3G55370.2 37.193 285 120 12 19 286 59 301 7.93e-38 138
MsaG036135 AT3G55370.1 40.553 217 90 7 19 235 59 236 9.15e-37 135
MsaG036135 AT3G55370.3 40.000 230 97 8 6 235 93 281 1.06e-36 135
MsaG036135 AT2G37590.1 72.152 79 20 1 16 94 69 145 6.22e-35 130
MsaG036135 AT1G07640.2 70.732 82 22 1 14 95 55 134 2.07e-34 129
MsaG036135 AT1G07640.1 72.727 77 19 1 19 95 4 78 2.99e-34 127
MsaG036135 AT1G07640.3 70.732 82 22 1 14 95 63 142 3.06e-34 128
MsaG036135 AT5G02460.1 70.130 77 21 1 16 92 75 149 1.04e-32 125
MsaG036135 AT2G28810.1 34.768 302 130 8 22 311 79 325 2.96e-32 123
MsaG036135 AT2G28810.2 34.768 302 130 8 22 311 62 308 3.33e-32 122
MsaG036135 AT1G51700.1 79.032 62 13 0 39 100 34 95 3.70e-31 116
MsaG036135 AT5G65590.1 80.328 61 12 0 39 99 42 102 1.69e-30 118
MsaG036135 AT5G60200.1 58.889 90 31 2 37 122 54 141 1.79e-30 116
MsaG036135 AT2G28510.1 80.952 63 10 1 37 99 47 107 3.35e-30 116
MsaG036135 AT1G21340.1 56.322 87 37 1 10 95 9 95 3.43e-30 115
MsaG036135 AT5G66940.1 52.294 109 45 2 40 141 34 142 5.60e-30 114
MsaG036135 AT5G60850.1 56.989 93 33 2 28 113 38 130 8.07e-30 116
MsaG036135 AT3G21270.1 83.929 56 9 0 39 94 30 85 1.44e-29 112
MsaG036135 AT3G45610.1 52.941 102 37 2 1 102 11 101 2.45e-29 113
MsaG036135 AT5G62940.1 88.235 51 6 0 38 88 73 123 2.14e-28 113
MsaG036135 AT4G38000.1 71.212 66 19 0 39 104 42 107 8.56e-28 109
MsaG036135 AT3G50410.1 75.439 57 14 0 40 96 32 88 1.61e-27 108
MsaG036135 AT1G28310.1 75.439 57 14 0 39 95 28 84 2.98e-27 109
MsaG036135 AT1G28310.2 75.439 57 14 0 39 95 52 108 3.99e-27 109
MsaG036135 AT3G52440.1 70.769 65 18 1 34 98 21 84 4.81e-27 107
MsaG036135 AT3G52440.2 70.769 65 18 1 34 98 40 103 7.06e-27 107
MsaG036135 AT4G00940.1 58.667 75 31 0 18 92 48 122 5.89e-26 105
MsaG036135 AT4G00940.3 58.667 75 31 0 18 92 48 122 5.89e-26 105
MsaG036135 AT4G00940.2 58.667 75 31 0 18 92 48 122 5.89e-26 105
MsaG036135 AT3G61850.2 62.857 70 26 0 40 109 64 133 1.09e-25 104
MsaG036135 AT3G61850.3 62.857 70 26 0 40 109 64 133 1.09e-25 104
MsaG036135 AT3G61850.1 62.857 70 26 0 40 109 76 145 1.19e-25 104
MsaG036135 AT4G21050.1 64.706 68 23 1 37 104 23 89 1.34e-25 102
MsaG036135 AT3G61850.5 62.857 70 26 0 40 109 64 133 1.70e-25 104
MsaG036135 AT3G61850.4 62.857 70 26 0 40 109 76 145 1.89e-25 104
MsaG036135 AT4G24060.1 75.000 56 14 0 37 92 52 107 3.30e-25 104
MsaG036135 AT1G47655.1 75.000 52 13 0 35 86 26 77 1.51e-23 96.7
MsaG036135 AT2G46590.1 84.783 46 7 0 40 85 70 115 2.67e-23 99.4
MsaG036135 AT2G46590.2 84.783 46 7 0 40 85 82 127 2.91e-23 99.4
MsaG036135 AT3G47500.1 74.510 51 13 0 40 90 112 162 3.55e-23 100
MsaG036135 AT1G29160.1 75.472 53 12 1 40 92 64 115 4.46e-23 94.7
MsaG036135 AT2G34140.1 75.472 53 12 1 40 92 60 111 5.14e-23 94.4
MsaG036135 AT1G64620.1 76.471 51 12 0 37 87 48 98 5.65e-23 98.2
MsaG036135 AT1G69570.1 74.510 51 13 0 40 90 134 184 1.27e-22 97.8
MsaG036135 AT5G39660.3 55.000 80 25 2 40 119 140 208 1.69e-22 98.2
MsaG036135 AT5G39660.1 55.000 80 25 2 40 119 140 208 1.69e-22 98.2
MsaG036135 AT5G39660.2 55.000 80 25 2 40 119 140 208 1.69e-22 98.2
MsaG036135 AT5G62430.1 54.167 72 32 1 19 90 36 106 1.98e-22 95.9
MsaG036135 AT4G21040.1 51.064 94 35 2 40 133 27 109 6.45e-22 93.2
MsaG036135 AT1G26790.1 50.633 79 37 1 12 90 87 163 9.36e-22 95.1
MsaG036135 AT4G21080.1 61.538 65 23 1 40 104 27 89 8.54e-21 90.5
MsaG036135 AT4G21030.1 57.692 52 22 0 40 91 23 74 2.74e-14 70.9

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAACTTGTCGTCGTTAT+TGG 0.174969 7:+27885273 MsaT036135.1:CDS
AAGAAATTTGTTTCGTTCTA+AGG 0.177053 7:+27885841 MsaT036135.1:CDS
GAAATAGTAATGATCTATTT+TGG 0.191335 7:+27885978 MsaT036135.1:CDS
CAAATTGGATTATTTCAATT+TGG 0.211059 7:+27885794 MsaT036135.1:CDS
AGTAATTTGCAACCACAAAT+TGG 0.232158 7:+27885779 MsaT036135.1:CDS
GCAAATTCTTCTTCTAATAA+TGG 0.247534 7:+27885458 MsaT036135.1:CDS
GTGCTGCTGCAGCATCATTC+TGG 0.303371 7:-27885169 None:intergenic
CAATTTGGTGGAGAAAATAA+TGG 0.326366 7:+27885809 MsaT036135.1:CDS
TGAGGGCATTGGTTCTTTGT+TGG 0.348674 7:+27885580 MsaT036135.1:CDS
TGGAGATGATCGTTATGATT+AGG 0.351307 7:-27885395 None:intergenic
CTTGGTAGTGGTTCTTCAAT+TGG 0.353175 7:+27885647 MsaT036135.1:CDS
TGATGAACTTGTAGCGAATT+CGG 0.372887 7:-27885728 None:intergenic
CAGGTCGGTGCCGGTGCTGC+TGG 0.390469 7:+27885416 MsaT036135.1:CDS
AATTGAAATAATCCAATTTG+TGG 0.391831 7:-27885791 None:intergenic
CTGTTGGAGGTGGTTGTAGA+AGG 0.393447 7:+27885324 MsaT036135.1:CDS
GAACGAAACAAATTTCTTTG+TGG 0.401844 7:-27885836 None:intergenic
CATTGTTCACCAATGCCATT+TGG 0.402281 7:-27885698 None:intergenic
GTGTTTGTTGATTCACATCT+TGG 0.421696 7:-27885200 None:intergenic
ATTGGATTATTTCAATTTGG+TGG 0.443223 7:+27885797 MsaT036135.1:CDS
TATTCTTCAAGTGTTAGTAT+TGG 0.447776 7:+27885869 MsaT036135.1:CDS
CAGCAGCACCGGCACCGACC+TGG 0.456075 7:-27885415 None:intergenic
AACCAAAAGCACTAGATTTG+AGG 0.456139 7:-27886076 None:intergenic
TCAAGTTAATACAGTTAAAG+TGG 0.456282 7:+27885901 MsaT036135.1:CDS
GGTTGCAAATTACTCAAGAA+AGG 0.462145 7:-27885770 None:intergenic
GTTACAAATGGAAATTGTGT+TGG 0.468460 7:-27885521 None:intergenic
CGATGTAAAGAGGTTACAAA+TGG 0.474364 7:-27885533 None:intergenic
CTTCTACAACCACCTCCAAC+AGG 0.475660 7:-27885323 None:intergenic
CGAAACGTTCCTGTTGGAGG+TGG 0.475928 7:+27885314 MsaT036135.1:CDS
TCTTCAATTGGTAATGGTAA+TGG 0.476358 7:+27885659 MsaT036135.1:CDS
AGTGGTTCTTCAATTGGTAA+TGG 0.485640 7:+27885653 MsaT036135.1:CDS
GCTCGATCGGTCATTGATAC+TGG 0.489149 7:-27885128 None:intergenic
CTTCGAAACGTTCCTGTTGG+AGG 0.496892 7:+27885311 MsaT036135.1:CDS
GTTGAGTTTCAACTTGGTAG+TGG 0.507591 7:+27885635 MsaT036135.1:CDS
ATACTGGTCTTCTAGACCCT+TGG 0.510341 7:-27885112 None:intergenic
AGTTATGTTTCTGAGGGCAT+TGG 0.510514 7:+27885569 MsaT036135.1:CDS
GTCGCTTTGACGATGTAAAG+AGG 0.511568 7:-27885543 None:intergenic
GTTGCAAATTACTCAAGAAA+GGG 0.514735 7:-27885769 None:intergenic
GGAAATTGTGTTGGAAAATG+AGG 0.514769 7:-27885512 None:intergenic
GATCATCTCCAGGTCGGTGC+CGG 0.519214 7:+27885407 MsaT036135.1:CDS
GGCTCTTTGTTGATACCAAA+TGG 0.520108 7:+27885683 MsaT036135.1:CDS
CTGGTGGTTCTAGCTCAGCA+AGG 0.527734 7:+27885435 MsaT036135.1:CDS
CGACAATAGTTATGTTTCTG+AGG 0.529372 7:+27885562 MsaT036135.1:CDS
GAAGCTGATGGCAGCTACCA+AGG 0.530104 7:+27885095 MsaT036135.1:CDS
GCTAGAACCACCAGCAGCAC+CGG 0.537679 7:-27885426 None:intergenic
GCACTTCGAAACGTTCCTGT+TGG 0.543248 7:+27885308 MsaT036135.1:CDS
ACTTTAACTGTATTAACTTG+AGG 0.543542 7:-27885899 None:intergenic
ACTAATTCTAATGTGAATGT+TGG 0.552195 7:+27885485 MsaT036135.1:CDS
CAAGGTGGTGAAGTGAAACT+AGG 0.554007 7:-27886049 None:intergenic
CACTAGATTTGAGGATCACA+AGG 0.554201 7:-27886067 None:intergenic
GACAATAGTTATGTTTCTGA+GGG 0.557481 7:+27885563 MsaT036135.1:CDS
GTGGAAGAAAACAATCATCA+AGG 0.560168 7:+27885920 MsaT036135.1:CDS
CTTTGACATCTTAGCTCGAT+CGG 0.562486 7:-27885141 None:intergenic
TAATCATAACGATCATCTCC+AGG 0.573464 7:+27885397 MsaT036135.1:CDS
GTCGGTGCCGGTGCTGCTGG+TGG 0.577708 7:+27885419 MsaT036135.1:CDS
TCAATTGGTAATGGTAATGG+TGG 0.579040 7:+27885662 MsaT036135.1:CDS
TCGTTATGATTAGGTTTCAG+AGG 0.584218 7:-27885386 None:intergenic
CGTCGTTATTGGACTAGAGG+TGG 0.585783 7:+27885284 MsaT036135.1:CDS
ACTAATGTTGAGTTTCAACT+TGG 0.589155 7:+27885629 MsaT036135.1:CDS
AAGCTGATGGCAGCTACCAA+GGG 0.593247 7:+27885096 MsaT036135.1:CDS
CAAATGGCATTGGTGAACAA+TGG 0.594140 7:+27885699 MsaT036135.1:CDS
TCTGTCAGCGTTGAAGCTGA+TGG 0.599724 7:+27885083 MsaT036135.1:CDS
TGTCGTCGTTATTGGACTAG+AGG 0.602398 7:+27885281 MsaT036135.1:CDS
ATGGCATTGGTGAACAATGG+AGG 0.607659 7:+27885702 MsaT036135.1:CDS
AATGGAAATGGAAATGGAAG+TGG 0.608244 7:+27886001 MsaT036135.1:CDS
GAAGTGGTAATGAAAATGCA+TGG 0.613061 7:+27886017 MsaT036135.1:CDS
TTGTTGATACCAAATGGCAT+TGG 0.626903 7:+27885689 MsaT036135.1:CDS
TGGTGGTTCTAGCTCAGCAA+GGG 0.640666 7:+27885436 MsaT036135.1:CDS
CATAACGATCATCTCCAGGT+CGG 0.682152 7:+27885401 MsaT036135.1:CDS
TAGATTTGAGGATCACAAGG+TGG 0.736101 7:-27886064 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 27885080 27886087 27885080 ID=MsaG036135
Chr7 mRNA 27885080 27886087 27885080 ID=MsaT036135.1;Parent=MsaG036135
Chr7 exon 27885080 27886087 27885080 ID=MsaT036135.1.exon1;Parent=MsaT036135.1
Chr7 CDS 27885080 27886087 27885080 ID=cds.MsaT036135.1;Parent=MsaT036135.1
Gene Sequence

>MsaT036135.1

ATGTCTGTCAGCGTTGAAGCTGATGGCAGCTACCAAGGGTCTAGAAGACCAGTATCAATGACCGATCGAGCTAAGATGTCAAAGATACACCAGAATGATGCTGCAGCAGCACAGAAATGTCCAAGATGTGAATCAACAAACACAAAGTTCTGTTACTACAACAACTACAATCTTTCACAACCACGCCATTTTTGCAAAACTTGTCGTCGTTATTGGACTAGAGGTGGTGCACTTCGAAACGTTCCTGTTGGAGGTGGTTGTAGAAGGAACAACAAAAGAAGAAAAAGTAATTCAGCATCAAAATCACCTCTGAAACCTAATCATAACGATCATCTCCAGGTCGGTGCCGGTGCTGCTGGTGGTTCTAGCTCAGCAAGGGCAAATTCTTCTTCTAATAATGGTTGCACTAATTCTAATGTGAATGTTGGAATGCCTCATTTTCCAACACAATTTCCATTTGTAACCTCTTTACATCGTCAAAGCGACAATAGTTATGTTTCTGAGGGCATTGGTTCTTTGTTGGCCAAAAATATGAGCAATAGTACTACAACTAATGTTGAGTTTCAACTTGGTAGTGGTTCTTCAATTGGTAATGGTAATGGTGGCTCTTTGTTGATACCAAATGGCATTGGTGAACAATGGAGGTTTCCGAATTCGCTACAAGTTCATCAACAACAATATCAGCAATTCCCTTTCTTGAGTAATTTGCAACCACAAATTGGATTATTTCAATTTGGTGGAGAAAATAATGGTGAACCACAAAGAAATTTGTTTCGTTCTAAGGAAACATATTCTTCAAGTGTTAGTATTGGAATGATGCCTCAAGTTAATACAGTTAAAGTGGAAGAAAACAATCATCAAGGTTTGAGTTTGCCAAAAAATCTCTTGAATGGTTCAGGAAATAGTAATGATCTATTTTGGAATGGAAATGGAAATGGAAGTGGTAATGAAAATGCATGGAATGAAGTTCCTAGTTTCACTTCACCACCTTGTGATCCTCAAATCTAG
Protein sequence

>MsaT036135.1

MSVSVEADGSYQGSRRPVSMTDRAKMSKIHQNDAAAAQKCPRCESTNTKFCYYNNYNLSQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKRRKSNSASKSPLKPNHNDHLQVGAGAAGGSSSARANSSSNNGCTNSNVNVGMPHFPTQFPFVTSLHRQSDNSYVSEGIGSLLAKNMSNSTTTNVEFQLGSGSSIGNGNGGSLLIPNGIGEQWRFPNSLQVHQQQYQQFPFLSNLQPQIGLFQFGGENNGEPQRNLFRSKETYSSSVSIGMMPQVNTVKVEENNHQGLSLPKNLLNGSGNSNDLFWNGNGNGSGNENAWNEVPSFTSPPCDPQI*