AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036150 MtrunA17_Chr1g0203051 42.953 149 85 0 1 149 70 218 9.32e-34 132
MsaG036150 MtrunA17_Chr1g0203051 32.759 116 76 2 151 265 507 621 9.44e-13 70.1
MsaG036150 MtrunA17_Chr1g0171741 46.825 126 66 1 11 136 134 258 7.91e-33 124
MsaG036150 MtrunA17_Chr2g0308841 46.512 129 65 2 151 277 100 226 1.11e-32 124
MsaG036150 MtrunA17_Chr8g0389811 40.496 121 72 0 1 121 75 195 1.70e-26 105
MsaG036150 MtrunA17_Chr2g0287651 34.483 145 95 0 1 145 66 210 4.29e-22 99.0
MsaG036150 MtrunA17_Chr2g0287651 30.457 197 117 4 151 339 505 689 1.05e-16 82.4
MsaG036150 MtrunA17_Chr2g0331371 43.103 116 66 0 6 121 51 166 1.22e-21 92.4
MsaG036150 MtrunA17_Chr6g0473421 45.000 80 44 0 6 85 51 130 1.82e-18 82.0
MsaG036150 MtrunA17_Chr1g0198761 35.570 149 80 5 16 152 53 197 7.15e-18 85.9
MsaG036150 MtrunA17_Chr2g0294841 34.459 148 83 5 15 152 50 193 6.60e-15 76.6
MsaG036150 MtrunA17_Chr1g0172931 42.529 87 50 0 6 92 72 158 1.31e-14 71.2
MsaG036150 MtrunA17_Chr2g0276841 31.690 142 90 4 16 152 76 215 7.43e-14 73.6
MsaG036150 MtrunA17_Chr1g0202341 28.986 138 95 2 8 144 49 184 2.14e-13 72.0
MsaG036150 MtrunA17_Chr2g0290391 32.867 143 85 3 16 152 57 194 2.20e-13 72.0
MsaG036150 MtrunA17_Chr5g0431001 33.333 147 86 4 16 152 67 211 6.24e-13 70.1
MsaG036150 MtrunA17_Chr5g0448761 30.216 139 95 2 8 144 58 196 1.30e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036150 AT5G28530.4 32.143 140 90 3 16 152 61 198 7.77e-17 82.8
MsaG036150 AT5G28530.5 32.143 140 90 3 16 152 61 198 8.23e-17 82.8
MsaG036150 AT5G28530.3 32.143 140 90 3 16 152 61 198 1.14e-16 82.4
MsaG036150 AT5G28530.2 32.143 140 90 3 16 152 61 198 1.14e-16 82.4
MsaG036150 AT5G28530.1 32.143 140 90 3 16 152 61 198 1.19e-16 82.4
MsaG036150 AT1G10240.2 31.690 142 90 4 16 152 54 193 1.58e-13 72.8
MsaG036150 AT1G10240.1 31.690 142 90 4 16 152 54 193 1.58e-13 72.8
MsaG036150 AT4G38180.1 31.206 141 94 3 6 144 69 208 5.13e-11 65.1

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGAACAGGACATATTT+TGG 0.217076 7:+28104666 None:intergenic
GGTTGCTAGGCATGCGAATT+TGG 0.260542 7:-28104387 MsaT036150.1:CDS
ACGAAGATGGTTCACATTAC+TGG 0.272975 7:-28104421 MsaT036150.1:CDS
AATTTGCAAAGGAGTCAATA+AGG 0.330201 7:-28104451 MsaT036150.1:CDS
ATGTTGCAAAGTTGCAATTT+GGG 0.334757 7:-28106106 MsaT036150.1:CDS
ACATTTGTTTGCAATCTAGA+AGG 0.335384 7:-28105975 MsaT036150.1:CDS
TTTGCAATCTAGAAGGGTTC+AGG 0.337269 7:-28105968 MsaT036150.1:CDS
GATGTTGCAAAGTTGCAATT+TGG 0.338447 7:-28106107 MsaT036150.1:CDS
CCAGAGAATTTACATGTTCC+AGG 0.348297 7:-28104242 MsaT036150.1:CDS
ATTCTCTGGTCCACCTTCAT+TGG 0.348700 7:+28104256 None:intergenic
ATCTAACAAATGAAATTAAC+AGG 0.356593 7:-28104295 MsaT036150.1:CDS
ACATTACTGGGATTCGCATT+TGG 0.373559 7:-28104408 MsaT036150.1:CDS
ATTAAGTACTTTGGTAATGC+TGG 0.376669 7:-28105729 MsaT036150.1:CDS
AAGATACTAGGGATACCTTT+TGG 0.380935 7:+28104699 None:intergenic
CATGAAACCCGTTGCGGTTG+TGG 0.386866 7:-28105900 MsaT036150.1:CDS
GATGGGTGATTACATCGATA+TGG 0.389759 7:+28105862 None:intergenic
CGAAGATGGTTCACATTACT+GGG 0.401396 7:-28104420 MsaT036150.1:CDS
GGGTGTCTAACAAAGATACT+AGG 0.406435 7:+28104687 None:intergenic
TGCATGTTCTTGTCTTAGAA+TGG 0.413399 7:-28104570 MsaT036150.1:CDS
AAGATTCAAATTAAGTACTT+TGG 0.415197 7:-28105738 MsaT036150.1:CDS
TCAACACTGTAGTCTCGTAT+TGG 0.428689 7:+28104801 None:intergenic
CAATGAATAAATTGTAGAAC+AGG 0.440957 7:+28104655 None:intergenic
CAACACTGTAGTCTCGTATT+GGG 0.441223 7:+28104802 None:intergenic
ATTTGCAAAGGAGTCAATAA+GGG 0.442855 7:-28104450 MsaT036150.1:CDS
CCGCAACGGGTTTCATGTAC+TGG 0.444224 7:+28105906 None:intergenic
GGGATTCGCATTTGGTTGCT+AGG 0.444717 7:-28104400 MsaT036150.1:CDS
CATCAACATCCTTGTAAGCT+AGG 0.445568 7:+28104339 None:intergenic
CAGTGTTGAAGACCAATCTT+AGG 0.451929 7:-28104787 MsaT036150.1:CDS
TTTACATGTTCCAGGGGAGT+TGG 0.463269 7:-28104234 MsaT036150.1:CDS
CCTGGAACATGTAAATTCTC+TGG 0.467772 7:+28104242 None:intergenic
GTGGGTTGTTGGCTGCTCAT+AGG 0.473653 7:-28105779 MsaT036150.1:CDS
TTGACAAAGTATCGAGATGA+AGG 0.474931 7:-28104635 MsaT036150.1:CDS
AGTTTCTGACCTAGCTTACA+AGG 0.475090 7:-28104348 MsaT036150.1:CDS
GTCTGTTTCGCATAGTCCAT+TGG 0.476618 7:-28104603 MsaT036150.1:CDS
CCTCTCAAGCGACCTAAGAT+TGG 0.477460 7:+28104775 None:intergenic
AAAGGGTGTGGGCATAGCAC+GGG 0.480342 7:-28104179 MsaT036150.1:CDS
GGTAATGCTGGAATCAAAGT+TGG 0.481875 7:-28105717 MsaT036150.1:CDS
ATCGAGATGAAGGTTATGTC+TGG 0.500336 7:-28104625 MsaT036150.1:CDS
AAAGTTGCAATTTGGGAGCC+TGG 0.501207 7:-28106099 MsaT036150.1:CDS
TGACAAACATTGTGGGTTGT+TGG 0.505785 7:-28105790 MsaT036150.1:CDS
CGAGCATATCATAGCGGTGC+TGG 0.506873 7:-28104528 MsaT036150.1:CDS
ATCAAAGTTGGTCAAATGAT+TGG 0.515889 7:-28105705 MsaT036150.1:intron
CTCGTATTGGGAATTAAGAT+CGG 0.525697 7:+28104814 None:intergenic
CCTTCATGTTCAAGAAGCAA+AGG 0.534160 7:-28104197 MsaT036150.1:CDS
TGTAGTATATGCGATGCAGC+TGG 0.535453 7:-28104122 MsaT036150.1:CDS
GGTACGATCTGTCATCCAAA+AGG 0.535783 7:-28104714 MsaT036150.1:CDS
ATAAGGGGAAATTACGAAGA+TGG 0.537087 7:-28104434 MsaT036150.1:CDS
CCTTTGCTTCTTGAACATGA+AGG 0.540439 7:+28104197 None:intergenic
CATTTGTTTGCAATCTAGAA+GGG 0.541599 7:-28105974 MsaT036150.1:CDS
AAATCAAAGTACTCCAATGA+AGG 0.541860 7:-28104269 MsaT036150.1:CDS
CAAAGGGTGTGGGCATAGCA+CGG 0.558534 7:-28104180 MsaT036150.1:CDS
AGTCTCTAGTGTTGAATCGT+TGG 0.565920 7:-28104478 MsaT036150.1:CDS
CTTCATGTTCAAGAAGCAAA+GGG 0.568108 7:-28104196 MsaT036150.1:CDS
TGTTCAAGAAGCAAAGGGTG+TGG 0.571034 7:-28104191 MsaT036150.1:CDS
ACGATTCAACACTAGAGACT+CGG 0.571760 7:+28104481 None:intergenic
CGATTCAACACTAGAGACTC+GGG 0.572407 7:+28104482 None:intergenic
GGGTGTGGGCATAGCACGGG+TGG 0.580265 7:-28104176 MsaT036150.1:CDS
GGTGTCTAACAAAGATACTA+GGG 0.580712 7:+28104688 None:intergenic
CAGAGAATTTACATGTTCCA+GGG 0.581872 7:-28104241 MsaT036150.1:CDS
AGTATATTATCCAACTCCCC+TGG 0.581886 7:+28104224 None:intergenic
GTTCAAGAAGCAAAGGGTGT+GGG 0.583267 7:-28104190 MsaT036150.1:CDS
GCATAGCACGGGTGGTACAA+TGG 0.586809 7:-28104168 MsaT036150.1:CDS
ACTTGTCATTGCTTAATCGT+AGG 0.589079 7:+28104080 None:intergenic
GAATTTGGTTTATCTTTGCA+AGG 0.595174 7:-28104372 MsaT036150.1:CDS
CCAATCTTAGGTCGCTTGAG+AGG 0.601635 7:-28104775 MsaT036150.1:CDS
TCAAAGTACTCCAATGAAGG+TGG 0.603482 7:-28104266 MsaT036150.1:CDS
CATGCAAATTGATTGTCCAA+TGG 0.608703 7:+28104587 None:intergenic
TCGTTGGTCACAATTTGCAA+AGG 0.617164 7:-28104462 MsaT036150.1:CDS
TGTTGAGTGACAAACATTGT+GGG 0.620764 7:-28105797 MsaT036150.1:CDS
TTTGCAAAGGAGTCAATAAG+GGG 0.629931 7:-28104449 MsaT036150.1:CDS
AGACAGAGGTCTAACAGCAG+AGG 0.634039 7:-28105943 MsaT036150.1:CDS
AACTGTAATGTACCAACGAT+GGG 0.642841 7:+28105845 None:intergenic
TCGATGTAATCACCCATCGT+TGG 0.645124 7:-28105857 MsaT036150.1:CDS
ATGTTGAGTGACAAACATTG+TGG 0.650930 7:-28105798 MsaT036150.1:CDS
ACAGCAGAGGAGCGTAAGCG+TGG 0.663483 7:-28105930 MsaT036150.1:CDS
AGAGAATTTACATGTTCCAG+GGG 0.665809 7:-28104240 MsaT036150.1:CDS
GTACAATGGAAAGACGTAGA+CGG 0.671436 7:-28104154 MsaT036150.1:CDS
GAAGGGTTCAGGAAAGACAG+AGG 0.686044 7:-28105957 MsaT036150.1:CDS
AAACTGTAATGTACCAACGA+TGG 0.697178 7:+28105844 None:intergenic
CCAGTACATGAAACCCGTTG+CGG 0.717976 7:-28105906 MsaT036150.1:CDS
TCATTGCGAGCATATCATAG+CGG 0.792144 7:-28104534 MsaT036150.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 28104085 28106156 28104085 ID=MsaG036150
Chr7 mRNA 28104085 28106156 28104085 ID=MsaT036150.1;Parent=MsaG036150
Chr7 exon 28104085 28104845 28104085 ID=MsaT036150.1.exon2;Parent=MsaT036150.1
Chr7 CDS 28104085 28104845 28104085 ID=cds.MsaT036150.1;Parent=MsaT036150.1
Chr7 exon 28105706 28106156 28105706 ID=MsaT036150.1.exon1;Parent=MsaT036150.1
Chr7 CDS 28105706 28106156 28105706 ID=cds.MsaT036150.1;Parent=MsaT036150.1
Gene Sequence

>MsaT036150.1

ATGAACATGTTTAACTTGCAAATTGAAGATGTTGCAAAGTTGCAATTTGGGAGCCTGGAAGTTGCCTACAATTTTTATTGTTGGTTTGCAAAGATGAACGATTTTGCTGTCAGAAAAGGTCAAGTTATTAAAAATAAGAACGGTGATGTTCTTCAACAAACATTTGTTTGCAATCTAGAAGGGTTCAGGAAAGACAGAGGTCTAACAGCAGAGGAGCGTAAGCGTGGTCCAGTACATGAAACCCGTTGCGGTTGTGGAGCAAAATTTCGAGTCCATATCGATGTAATCACCCATCGTTGGTACATTACAGTTTTCATCTTTGATCACAATCATGAAATGTTGAGTGACAAACATTGTGGGTTGTTGGCTGCTCATAGGAAGCTCAGTGAGTCAGACAAGATTCAAATTAAGTACTTTGGTAATGCTGGAATCAAAGTTGGTCAAATGATTGAGGTTCAAGCCGATCTTAATTCCCAATACGAGACTACAGTGTTGAAGACCAATCTTAGGTCGCTTGAGAGGTCTGCCTCAAAACAGTTTACCAAAGAAATTTTTGAGATGGTACGATCTGTCATCCAAAAGGTATCCCTAGTATCTTTGTTAGACACCCAAAATATGTCCTGTTCTACAATTTATTCATTGACAAAGTATCGAGATGAAGGTTATGTCTGGTCTGTTTCGCATAGTCCATTGGACAATCAATTTGCATGTTCTTGTCTTAGAATGGAATCTATAAGAATTCATTGCGAGCATATCATAGCGGTGCTGGTATATCTTGATTTTGTTGATTTTCCCGAGTCTCTAGTGTTGAATCGTTGGTCACAATTTGCAAAGGAGTCAATAAGGGGAAATTACGAAGATGGTTCACATTACTGGGATTCGCATTTGGTTGCTAGGCATGCGAATTTGGTTTATCTTTGCAAGGAAGTTTCTGACCTAGCTTACAAGGATGTTGATGATTATAAAAATTATTTGGAATATCTAACAAATGAAATTAACAGGCTTAAATCAAAGTACTCCAATGAAGGTGGACCAGAGAATTTACATGTTCCAGGGGAGTTGGATAATATACTTAATCCTTCATGTTCAAGAAGCAAAGGGTGTGGGCATAGCACGGGTGGTACAATGGAAAGACGTAGACGGACACAGACTTGTAGTATATGCGATGCAGCTGGACACAACAAACGATCTTGTCCTACGATTAAGCAATGA

Protein sequence

>MsaT036150.1

MNMFNLQIEDVAKLQFGSLEVAYNFYCWFAKMNDFAVRKGQVIKNKNGDVLQQTFVCNLEGFRKDRGLTAEERKRGPVHETRCGCGAKFRVHIDVITHRWYITVFIFDHNHEMLSDKHCGLLAAHRKLSESDKIQIKYFGNAGIKVGQMIEVQADLNSQYETTVLKTNLRSLERSASKQFTKEIFEMVRSVIQKVSLVSLLDTQNMSCSTIYSLTKYRDEGYVWSVSHSPLDNQFACSCLRMESIRIHCEHIIAVLVYLDFVDFPESLVLNRWSQFAKESIRGNYEDGSHYWDSHLVARHANLVYLCKEVSDLAYKDVDDYKNYLEYLTNEINRLKSKYSNEGGPENLHVPGELDNILNPSCSRSKGCGHSTGGTMERRRRTQTCSICDAAGHNKRSCPTIKQ*