AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036153


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036153 MtrunA17_Chr2g0308841 53.216 171 80 0 257 427 56 226 9.34e-64 208
MsaG036153 MtrunA17_Chr1g0203051 39.524 210 122 2 28 237 34 238 1.73e-42 160
MsaG036153 MtrunA17_Chr1g0203051 34.595 185 118 1 234 415 437 621 5.49e-28 117
MsaG036153 MtrunA17_Chr1g0171741 36.041 197 124 2 11 206 65 260 3.30e-36 135
MsaG036153 MtrunA17_Chr8g0389811 42.188 128 74 0 55 182 61 188 2.58e-32 122
MsaG036153 MtrunA17_Chr2g0331371 38.608 158 95 1 27 182 2 159 5.95e-26 105
MsaG036153 MtrunA17_Chr2g0287651 33.333 165 110 0 73 237 70 234 1.04e-25 111
MsaG036153 MtrunA17_Chr2g0287651 35.260 173 112 0 256 428 460 632 1.33e-24 107
MsaG036153 MtrunA17_Chr6g0473421 38.760 129 77 1 27 153 2 130 8.11e-24 97.4
MsaG036153 MtrunA17_Chr5g0448761 30.061 163 112 2 77 237 59 221 2.85e-17 85.1
MsaG036153 MtrunA17_Chr1g0202341 27.919 197 129 4 50 237 15 207 1.40e-16 82.8
MsaG036153 MtrunA17_Chr1g0172931 36.634 101 64 0 60 160 58 158 3.45e-16 75.9
MsaG036153 MtrunA17_Chr1g0198761 35.252 139 78 6 90 218 59 195 1.11e-14 77.0
MsaG036153 MtrunA17_Chr2g0276841 31.429 140 89 4 84 218 76 213 9.31e-14 73.9
MsaG036153 MtrunA17_Chr2g0294841 32.414 145 86 5 83 218 50 191 1.56e-13 73.2
MsaG036153 MtrunA17_Chr3g0118611 23.889 180 132 3 276 453 350 526 1.29e-11 67.0
MsaG036153 MtrunA17_Chr5g0431001 25.688 218 129 5 54 250 25 230 2.07e-11 66.2
MsaG036153 MtrunA17_Chr6g0458391 23.741 278 170 11 6 250 10 278 2.67e-11 66.2
MsaG036153 MtrunA17_Chr2g0324011 24.242 198 140 3 245 438 391 582 3.64e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036153 AT4G38180.1 30.000 170 114 4 70 237 67 233 2.81e-16 82.4
MsaG036153 AT4G38180.1 25.263 190 134 4 263 447 470 656 1.98e-12 70.1
MsaG036153 AT1G10240.2 28.421 190 118 7 35 218 14 191 8.70e-16 80.9
MsaG036153 AT1G10240.1 28.421 190 118 7 35 218 14 191 8.70e-16 80.9
MsaG036153 AT5G28530.4 32.061 131 86 2 90 218 67 196 1.51e-14 76.6
MsaG036153 AT5G28530.5 32.061 131 86 2 90 218 67 196 1.54e-14 76.6
MsaG036153 AT5G28530.3 32.061 131 86 2 90 218 67 196 1.56e-14 76.6
MsaG036153 AT5G28530.2 32.061 131 86 2 90 218 67 196 1.56e-14 76.6
MsaG036153 AT5G28530.1 32.061 131 86 2 90 218 67 196 1.65e-14 76.6
MsaG036153 AT4G38170.1 25.424 177 129 2 258 434 230 403 2.29e-11 66.6

Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGTTTGCAAAGATTAA+TGG 0.202245 7:-28116753 MsaT036153.1:CDS
GAGTATCTAATAATGATATT+AGG 0.223767 7:+28115401 None:intergenic
CTTACTAAGATTCACCAAAT+TGG 0.273526 7:+28115087 None:intergenic
AGTTTCTGATTTGGCATATC+AGG 0.280882 7:-28115062 MsaT036153.1:CDS
TGTCTTAGAATGGAATCTAT+TGG 0.286177 7:-28115274 MsaT036153.1:CDS
GCTGATCACCAATCTCAATA+TGG 0.303020 7:-28115529 MsaT036153.1:CDS
GGTTGCGAGACACGCCAATT+TGG 0.319259 7:-28115101 MsaT036153.1:CDS
CTATGGCAATGCTGGAATAA+TGG 0.328742 7:-28116418 MsaT036153.1:CDS
GTCTTAGAATGGAATCTATT+GGG 0.342081 7:-28115273 MsaT036153.1:CDS
TGTGGGCAACATCTCATGTT+AGG 0.342490 7:-28115688 MsaT036153.1:CDS
CCACACCTGGTTTCGTGTCT+TGG 0.351926 7:+28116603 None:intergenic
ATCAAGATGGTTCACACTAC+TGG 0.359918 7:-28115135 MsaT036153.1:CDS
AGTATCTAATAATGATATTA+GGG 0.360092 7:+28115402 None:intergenic
TGTGTAATCTAGAAGGATTT+AGG 0.362208 7:-28116665 MsaT036153.1:CDS
TAGTAAGGAAGTTTCTGATT+TGG 0.367852 7:-28115071 MsaT036153.1:CDS
ACACTACTGGGATTCACATT+TGG 0.373928 7:-28115122 MsaT036153.1:CDS
TGACACCGCTGGTGACGATT+TGG 0.375975 7:-28116946 MsaT036153.1:CDS
CTTTAATAACGTGACCTTTG+CGG 0.378527 7:+28116721 None:intergenic
TAAATGCACTTGTCTTAGAA+TGG 0.394455 7:-28115284 MsaT036153.1:CDS
AGAGAAGCATTGCGGGTTGT+TGG 0.401759 7:-28116487 MsaT036153.1:CDS
ATCGATAACTATGGCAATGC+TGG 0.402549 7:-28116426 MsaT036153.1:CDS
GTGCATATTGATATAAATTC+AGG 0.402681 7:-28116561 MsaT036153.1:CDS
AAATATCGAGACGAGGGATA+TGG 0.410059 7:-28115343 MsaT036153.1:CDS
TGTTAATAGAGAAGCATTGC+GGG 0.410334 7:-28116494 MsaT036153.1:CDS
AAGATTCAAATCGATAACTA+TGG 0.410922 7:-28116435 MsaT036153.1:CDS
TGATTCAGCACAAGATACTT+AGG 0.418044 7:+28115196 None:intergenic
CACAAAAGAAATATTCATCT+TGG 0.422432 7:-28115449 MsaT036153.1:CDS
GCTTCTCTATTAACATATCA+TGG 0.424924 7:+28116502 None:intergenic
TCGAGGTTTAGCCATGAAAA+TGG 0.425862 7:-28114980 MsaT036153.1:CDS
AGTATCTTGTGCTGAATCAT+TGG 0.438285 7:-28115192 MsaT036153.1:CDS
TCAATTAAGTAAGATTCGTC+TGG 0.440807 7:+28116876 None:intergenic
TCAATGAGATGTTTGAGAAA+AGG 0.443065 7:-28115715 MsaT036153.1:CDS
CAGTGTTGCAGACCAGTCTA+AGG 0.459285 7:-28115501 MsaT036153.1:CDS
TTGATATAAATTCAGGTCGT+TGG 0.459962 7:-28116554 MsaT036153.1:CDS
GTGGGCAACATCTCATGTTA+GGG 0.462616 7:-28115687 MsaT036153.1:CDS
TTCATCTTTGAATGTACAAA+CGG 0.464037 7:+28115616 None:intergenic
GCTGGTGGGTATGATAAGGT+TGG 0.466209 7:-28116363 MsaT036153.1:CDS
TTGAATATAGACAACATTGA+TGG 0.468403 7:-28117029 MsaT036153.1:CDS
CAATTTGGTGAATCTTAGTA+AGG 0.472298 7:-28115086 MsaT036153.1:CDS
CTGATCACCAATCTCAATAT+GGG 0.482888 7:-28115528 MsaT036153.1:CDS
CACGTTATTAAAGATAAGAA+TGG 0.499881 7:-28116711 MsaT036153.1:CDS
GTCAATGTAACTCATGATGA+TGG 0.504196 7:-28116999 MsaT036153.1:CDS
CTGGTGGGTATGATAAGGTT+GGG 0.505137 7:-28116362 MsaT036153.1:CDS
GTCTACTCAGTTCATCAGTA+AGG 0.506109 7:+28115008 None:intergenic
CACGAAACCAGGTGTGGTTG+TGG 0.506910 7:-28116597 MsaT036153.1:CDS
TTTCACGCCACAACCACACC+TGG 0.510809 7:+28116590 None:intergenic
GGTGCATTTGCAAATGCTGC+TGG 0.519175 7:-28116381 MsaT036153.1:CDS
CCTCTCGAGCGACCTTAGAC+TGG 0.519542 7:+28115489 None:intergenic
TGTTTGAGAAAAGGAATATG+TGG 0.523368 7:-28115706 MsaT036153.1:CDS
TGTAGACAATATTGTGAAGA+TGG 0.524311 7:-28116850 MsaT036153.1:CDS
GATTCAGCACAAGATACTTA+GGG 0.524884 7:+28115197 None:intergenic
CTGCCTGCCCATATTGAGAT+TGG 0.525441 7:+28115521 None:intergenic
GCGGGTTGTTGGCTGCTCAC+AGG 0.526567 7:-28116476 MsaT036153.1:CDS
TCAAGATGGTTCACACTACT+GGG 0.549463 7:-28115134 MsaT036153.1:CDS
TGTCAAGGAATAAATATCAA+AGG 0.551099 7:+28115366 None:intergenic
ATAATGGTTACTCAAATGAT+TGG 0.552942 7:-28116402 MsaT036153.1:CDS
AACCATCTTGATAGTTTCCA+CGG 0.556190 7:+28115146 None:intergenic
GAAAATATTCTACACACTGG+TGG 0.557474 7:+28115569 None:intergenic
CATTTAAATTCATCGTTGAG+TGG 0.559914 7:+28115301 None:intergenic
TTGGCCTGGAAGAAAACAGT+TGG 0.561624 7:-28115739 MsaT036153.1:intron
TCACCAATCTCAATATGGGC+AGG 0.573156 7:-28115524 MsaT036153.1:CDS
GTTTGAGAAAAGGAATATGT+GGG 0.574502 7:-28115705 MsaT036153.1:CDS
ATCCGTGGAAACTATCAAGA+TGG 0.579257 7:-28115148 MsaT036153.1:CDS
GCATTTGCAAATGCTGCTGG+TGG 0.585164 7:-28116378 MsaT036153.1:CDS
TCAATGTAACTCATGATGAT+GGG 0.586905 7:-28116998 MsaT036153.1:CDS
CCAGTCTAAGGTCGCTCGAG+AGG 0.592281 7:-28115489 MsaT036153.1:CDS
TGCTGCTGGTGGGTATGATA+AGG 0.594589 7:-28116367 MsaT036153.1:CDS
GATCCCTTGTGAGCATATTG+TGG 0.601137 7:-28115251 MsaT036153.1:CDS
GTGGTCCAAGACACGAAACC+AGG 0.602270 7:-28116608 MsaT036153.1:CDS
GCAAACGACAATGACACCGC+TGG 0.611845 7:-28116957 MsaT036153.1:CDS
CATTTGCAAATGCTGCTGGT+GGG 0.618076 7:-28116377 MsaT036153.1:CDS
TCATTGGTCACAATTTGCAA+AGG 0.618936 7:-28115176 MsaT036153.1:CDS
CTTGACAAAATATCGAGACG+AGG 0.625437 7:-28115350 MsaT036153.1:CDS
TGAAGCATTTCATAGCCACA+TGG 0.625788 7:-28115641 MsaT036153.1:CDS
ATCGAAAATATTCTACACAC+TGG 0.626984 7:+28115566 None:intergenic
ACCGCCACAATATGCTCACA+AGG 0.627656 7:+28115247 None:intergenic
TTCATCCAAATCGTCACCAG+CGG 0.634435 7:+28116941 None:intergenic
ATAGACAACATTGATGGTGA+AGG 0.635617 7:-28117023 MsaT036153.1:CDS
ATGTTAATAGAGAAGCATTG+CGG 0.638277 7:-28116495 MsaT036153.1:CDS
AACTGAGTAGACTGAAATCG+AGG 0.648645 7:-28114997 MsaT036153.1:CDS
AAGGATTTAGGTATGACAGA+GGG 0.653871 7:-28116653 MsaT036153.1:CDS
AATAACGTGACCTTTGCGGA+CGG 0.654807 7:+28116725 None:intergenic
TTGACAAAATATCGAGACGA+GGG 0.659491 7:-28115349 MsaT036153.1:CDS
CCCTTGTGAGCATATTGTGG+CGG 0.661298 7:-28115248 MsaT036153.1:CDS
CAATGTAACTCATGATGATG+GGG 0.663298 7:-28116997 MsaT036153.1:CDS
TTTGCAAAGGAGTCTATCCG+TGG 0.667803 7:-28115163 MsaT036153.1:CDS
TGGGCAACATCTCATGTTAG+GGG 0.677310 7:-28115686 MsaT036153.1:CDS
GGGCAACATCTCATGTTAGG+GGG 0.677920 7:-28115685 MsaT036153.1:CDS
ACATCAGAGAAACGCAAGCG+TGG 0.681208 7:-28116627 MsaT036153.1:CDS
GAAGGATTTAGGTATGACAG+AGG 0.687850 7:-28116654 MsaT036153.1:CDS
CCAAGACACGAAACCAGGTG+TGG 0.688722 7:-28116603 MsaT036153.1:CDS
GAAGCATTTCATAGCCACAT+GGG 0.694477 7:-28115640 MsaT036153.1:CDS
GGGGAATACGAACTACATCA+GGG 0.695608 7:-28115666 MsaT036153.1:CDS
AATGTACAAACGGACCCATG+TGG 0.708271 7:+28115626 None:intergenic
CCGCCACAATATGCTCACAA+GGG 0.726641 7:+28115248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 28114973 28117057 28114973 ID=MsaG036153
Chr7 mRNA 28114973 28117057 28114973 ID=MsaT036153.1;Parent=MsaG036153
Chr7 exon 28114973 28115749 28114973 ID=MsaT036153.1.exon2;Parent=MsaT036153.1
Chr7 CDS 28114973 28115749 28114973 ID=cds.MsaT036153.1;Parent=MsaT036153.1
Chr7 exon 28116347 28117057 28116347 ID=MsaT036153.1.exon1;Parent=MsaT036153.1
Chr7 CDS 28116347 28117057 28116347 ID=cds.MsaT036153.1;Parent=MsaT036153.1
Gene Sequence

>MsaT036153.1

ATGGATTTGAATATAGACAACATTGATGGTGAAGGTGTCAATGTAACTCATGATGATGGGGAGTGTTCTGATAGCGATGCAAACGACAATGACACCGCTGGTGACGATTTGGATGAAAATTCAAACAGCTTCAATGAATCAACACACTCAGATCAAAATCCAGACGAATCTTACTTAATTGATGATGTAGACAATATTGTGAAGATGGATATGTTTAACTTGAAGAATGAAGATGTTTCGAAGTTGCAATTTCGTAATCTTGAAGTTGCGTACTCATTTTTTTGTTGGTTTGCAAAGATTAATGGTTTTGCCGTCCGCAAAGGTCACGTTATTAAAGATAAGAATGGAGATGTTGTTCAACAAACTTTTGTGTGTAATCTAGAAGGATTTAGGTATGACAGAGGGTTAACATCAGAGAAACGCAAGCGTGGTCCAAGACACGAAACCAGGTGTGGTTGTGGCGTGAAATTTCGAGTGCATATTGATATAAATTCAGGTCGTTGGTACATCACACTTTTCAGTTATGAGCACAACCATGATATGTTAATAGAGAAGCATTGCGGGTTGTTGGCTGCTCACAGGAAACTGACAAAGTCAGATAAGATTCAAATCGATAACTATGGCAATGCTGGAATAATGGTTACTCAAATGATTGGTGCATTTGCAAATGCTGCTGGTGGGTATGATAAGGTTGGGTTATTGAAGAAAGATAAAACAGTTGGATCAATGAGATGTTTGAGAAAAGGAATATGTGGGCAACATCTCATGTTAGGGGGAATACGAACTACATCAGGGTGTGAAGCATTTCATAGCCACATGGGTCCGTTTGTACATTCAAAGATGAATATGACTGATTTTGTAAAGCAGTTCCACCAGTGTGTAGAATATTTTCGATTTAGAGAGGTTCAAGCTGATCACCAATCTCAATATGGGCAGGCAGTGTTGCAGACCAGTCTAAGGTCGCTCGAGAGGTCAGCTTCAAAACAATTCACAAAAGAAATATTCATCTTGGTACATTTTGTCCTTAAAAAGGTTTCCCTAATATCATTATTAGATACTCAACATATGTCATCCTTTGATATTTATTCCTTGACAAAATATCGAGACGAGGGATATGGTTTTCGAGTTTCGCATTGTCCACTCAACGATGAATTTAAATGCACTTGTCTTAGAATGGAATCTATTGGGATCCCTTGTGAGCATATTGTGGCGGTGCTAGTATATCTTGATTTTGCTGATTTCCCTAAGTATCTTGTGCTGAATCATTGGTCACAATTTGCAAAGGAGTCTATCCGTGGAAACTATCAAGATGGTTCACACTACTGGGATTCACATTTGGTTGCGAGACACGCCAATTTGGTGAATCTTAGTAAGGAAGTTTCTGATTTGGCATATCAGGATGTTGATGATTATAAAAATTATTTAAATTACCTTACTGATGAACTGAGTAGACTGAAATCGAGGTTTAGCCATGAAAATGGTCCATAG

Protein sequence

>MsaT036153.1

MDLNIDNIDGEGVNVTHDDGECSDSDANDNDTAGDDLDENSNSFNESTHSDQNPDESYLIDDVDNIVKMDMFNLKNEDVSKLQFRNLEVAYSFFCWFAKINGFAVRKGHVIKDKNGDVVQQTFVCNLEGFRYDRGLTSEKRKRGPRHETRCGCGVKFRVHIDINSGRWYITLFSYEHNHDMLIEKHCGLLAAHRKLTKSDKIQIDNYGNAGIMVTQMIGAFANAAGGYDKVGLLKKDKTVGSMRCLRKGICGQHLMLGGIRTTSGCEAFHSHMGPFVHSKMNMTDFVKQFHQCVEYFRFREVQADHQSQYGQAVLQTSLRSLERSASKQFTKEIFILVHFVLKKVSLISLLDTQHMSSFDIYSLTKYRDEGYGFRVSHCPLNDEFKCTCLRMESIGIPCEHIVAVLVYLDFADFPKYLVLNHWSQFAKESIRGNYQDGSHYWDSHLVARHANLVNLSKEVSDLAYQDVDDYKNYLNYLTDELSRLKSRFSHENGP*