AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036172 MtrunA17_Chr7g0222981 97.268 183 5 0 1 183 1 183 1.52e-131 366
MsaG036172 MtrunA17_Chr1g0212511 61.290 186 50 5 1 165 5 189 4.42e-67 206
MsaG036172 MtrunA17_Chr3g0144021 45.026 191 79 4 9 176 7 194 1.07e-45 149
MsaG036172 MtrunA17_Chr8g0392991 48.387 62 32 0 68 129 96 157 3.68e-13 66.6
MsaG036172 MtrunA17_Chr4g0043891 39.773 88 48 1 68 150 8 95 6.41e-13 65.1
MsaG036172 MtrunA17_Chr2g0283141 41.935 62 36 0 66 127 53 114 1.06e-11 62.4
MsaG036172 MtrunA17_Chr4g0031341 45.161 62 34 0 68 129 25 86 6.85e-11 59.3
MsaG036172 MtrunA17_Chr5g0436661 31.193 109 60 2 68 175 33 127 9.03e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036172 AT4G35550.1 57.237 152 45 2 17 148 33 184 4.84e-47 155
MsaG036172 AT1G20700.3 47.333 150 56 4 15 141 24 173 6.29e-36 124
MsaG036172 AT1G20700.1 47.333 150 56 4 15 141 24 173 8.63e-36 124
MsaG036172 AT1G20710.1 46.457 127 48 2 21 128 14 139 1.81e-25 97.4
MsaG036172 AT1G20700.2 54.321 81 32 1 66 141 31 111 7.38e-23 89.4
MsaG036172 AT3G18010.1 45.161 62 34 0 68 129 76 137 4.92e-12 63.5
MsaG036172 AT2G33880.1 41.935 62 36 0 66 127 53 114 2.22e-11 62.0
MsaG036172 AT2G33880.2 41.935 62 36 0 66 127 53 114 2.53e-11 61.6
MsaG036172 AT5G45980.1 41.935 62 36 0 66 127 53 114 5.60e-11 60.5

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGACCAGTGTGTATAAT+TGG 0.216618 7:+28443184 MsaT036172.1:CDS
ACCTGTGCAACTTCAAAGTT+TGG 0.323786 7:+28443074 MsaT036172.1:CDS
TGTATAATTGGTTTCAGAAT+AGG 0.361284 7:+28443196 MsaT036172.1:CDS
GAGATCTTGTTGAGAAGATA+AGG 0.380614 7:-28442821 None:intergenic
TCCTAGTAAGGAAAAGATAA+AGG 0.397905 7:+28443125 MsaT036172.1:CDS
TACACACTGGTCTCTGAAAT+CGG 0.399480 7:-28443177 None:intergenic
CTCTGAAATCGGCCCGTGCT+TGG 0.426359 7:-28443166 None:intergenic
TCAACCTCAGTCTCTACTTC+CGG 0.438371 7:-28443264 None:intergenic
AAATAATGTTAATGATGAAC+CGG 0.459463 7:+28443245 MsaT036172.1:CDS
GACTGAGATGCAACGATCTC+CGG 0.461176 7:-28443309 None:intergenic
CGAATATTTGAAGCTGAGAC+GGG 0.462716 7:+28443099 MsaT036172.1:CDS
ATGAACAAGTCGAAACTCTT+CGG 0.463263 7:+28442743 MsaT036172.1:CDS
TCGTAAACTGTCATCTGATT+CGG 0.469903 7:-28443373 None:intergenic
CACTTGCATCATTCCAACAT+TGG 0.480071 7:-28442707 None:intergenic
AATTCAAGTCCGCTAAGTAC+TGG 0.486943 7:-28443350 None:intergenic
CTACTAGGCTGAGGCAGCGT+TGG 0.489931 7:+28443043 MsaT036172.1:CDS
CAATGATAAGAAAACTGAAC+CGG 0.495461 7:+28443290 MsaT036172.1:CDS
AAGAGTTTCGACTTGTTCAT+CGG 0.503160 7:-28442740 None:intergenic
GGTTTCAGAATAGGCGTGCT+AGG 0.518890 7:+28443205 MsaT036172.1:CDS
GCGAATATTTGAAGCTGAGA+CGG 0.525730 7:+28443098 MsaT036172.1:CDS
TGAGACGGGGACTCCTAGTA+AGG 0.526200 7:+28443113 MsaT036172.1:CDS
TCCAAACTTTGAAGTTGCAC+AGG 0.527601 7:-28443075 None:intergenic
TTTAATCAGAACGCCAATGT+TGG 0.533338 7:+28442694 MsaT036172.1:CDS
GAATAGGCGTGCTAGGTCAA+AGG 0.534265 7:+28443212 MsaT036172.1:CDS
AATAGGCGTGCTAGGTCAAA+GGG 0.534387 7:+28443213 MsaT036172.1:CDS
CAACAAGATCTCACTCTCAC+TGG 0.535564 7:+28442832 MsaT036172.1:CDS
TTTGTGCAGCCAGTACTTAG+CGG 0.536719 7:+28443341 MsaT036172.1:CDS
CTTTGAAGTTGCACAGGTGT+TGG 0.537712 7:-28443069 None:intergenic
ACCGCTGACTTAACCAAGCA+CGG 0.545012 7:+28443153 MsaT036172.1:CDS
CCCGTGCTTGGTTAAGTCAG+CGG 0.597671 7:-28443154 None:intergenic
CCGCTGACTTAACCAAGCAC+GGG 0.640067 7:+28443154 MsaT036172.1:CDS
CTGAAACCAATTATACACAC+TGG 0.642488 7:-28443190 None:intergenic
GAATATTTGAAGCTGAGACG+GGG 0.658394 7:+28443100 MsaT036172.1:CDS
TGAACCGGAAGTAGAGACTG+AGG 0.698771 7:+28443260 MsaT036172.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 28442673 28443410 28442673 ID=MsaG036172
Chr7 mRNA 28442673 28443410 28442673 ID=MsaT036172.1;Parent=MsaG036172
Chr7 exon 28442673 28442853 28442673 ID=MsaT036172.1.exon1;Parent=MsaT036172.1
Chr7 CDS 28442673 28442853 28442673 ID=cds.MsaT036172.1;Parent=MsaT036172.1
Chr7 exon 28443040 28443410 28443040 ID=MsaT036172.1.exon2;Parent=MsaT036172.1
Chr7 CDS 28443040 28443410 28443040 ID=cds.MsaT036172.1;Parent=MsaT036172.1
Gene Sequence

>MsaT036172.1

ATGGAGTGGCAGAAGAACTGTTTTAATCAGAACGCCAATGTTGGAATGATGCAAGTGAATGTGATGACCGATGAACAAGTCGAAACTCTTCGGAAACAAATAGCAGTTTATTCTGTAATCTCTGAACAACTCATTCAAACGCATAAAACCTTATCTTCTCAACAAGATCTCACTCTCACTGGGTCTACTAGGCTGAGGCAGCGTTGGACACCAACACCTGTGCAACTTCAAAGTTTGGAGCGAATATTTGAAGCTGAGACGGGGACTCCTAGTAAGGAAAAGATAAAGGAGATAACCGCTGACTTAACCAAGCACGGGCCGATTTCAGAGACCAGTGTGTATAATTGGTTTCAGAATAGGCGTGCTAGGTCAAAGGGAAAGCAGCAAAATAATGTTAATGATGAACCGGAAGTAGAGACTGAGGTTGATTCCAATGATAAGAAAACTGAACCGGAGATCGTTGCATCTCAGTCTGCTCAAAATTTGTGCAGCCAGTACTTAGCGGACTTGAATTCCGAATCAGATGACAGTTTACGACGCTCTAGAAACTGA
Protein sequence

>MsaT036172.1

MEWQKNCFNQNANVGMMQVNVMTDEQVETLRKQIAVYSVISEQLIQTHKTLSSQQDLTLTGSTRLRQRWTPTPVQLQSLERIFEAETGTPSKEKIKEITADLTKHGPISETSVYNWFQNRRARSKGKQQNNVNDEPEVETEVDSNDKKTEPEIVASQSAQNLCSQYLADLNSESDDSLRRSRN*