AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036264

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036264 MtrunA17_Chr7g0224051 90.128 1175 83 4 46 1217 1 1145 0.0 2165
MsaG036264 MtrunA17_Chr3g0114161 75.122 1226 245 14 20 1188 25 1247 0.0 1798
MsaG036264 MtrunA17_Chr1g0191141 69.397 879 245 11 322 1189 238 1103 0.0 1194
MsaG036264 MtrunA17_Chr1g0191141 59.402 234 84 4 20 247 8 236 1.54e-73 266
MsaG036264 MtrunA17_Chr5g0437621 63.213 859 281 13 355 1187 453 1302 0.0 1064
MsaG036264 MtrunA17_Chr5g0437621 43.731 327 154 8 4 313 3 316 3.28e-65 242
MsaG036264 MtrunA17_Chr7g0233021 36.630 718 388 22 478 1169 537 1213 2.90e-113 383
MsaG036264 MtrunA17_Chr7g0233031 33.826 745 417 19 460 1175 535 1232 8.09e-113 382
MsaG036264 MtrunA17_Chr7g0225791 33.511 752 439 17 460 1188 577 1290 5.65e-112 380
MsaG036264 MtrunA17_Chr7g0224031 81.279 219 39 2 751 968 25 242 7.60e-112 349
MsaG036264 MtrunA17_Chr7g0232971 33.506 773 440 21 445 1188 571 1298 5.88e-108 369
MsaG036264 MtrunA17_Chr4g0047081 33.836 730 427 15 478 1188 548 1240 1.18e-107 367
MsaG036264 MtrunA17_Chr7g0232991 33.122 631 356 15 595 1188 61 662 1.52e-93 315
MsaG036264 MtrunA17_Chr2g0296421 31.330 699 387 20 457 1144 385 1001 1.28e-88 310
MsaG036264 MtrunA17_Chr8g0352361 30.835 707 414 18 443 1144 216 852 1.39e-88 306
MsaG036264 MtrunA17_Chr7g0233001 32.907 547 328 12 657 1188 3 525 8.56e-76 261
MsaG036264 MtrunA17_Chr7g0224021 76.991 113 24 1 576 688 1 111 5.90e-54 184
MsaG036264 MtrunA17_Chr2g0320781 33.745 243 154 4 896 1137 4 240 2.13e-37 141
MsaG036264 MtrunA17_Chr5g0393461 23.284 670 380 24 569 1196 163 740 1.30e-31 134
MsaG036264 MtrunA17_Chr5g0403921 26.884 584 336 20 641 1209 206 713 2.81e-30 129
MsaG036264 MtrunA17_Chr8g0384531 24.957 581 326 22 594 1136 372 880 1.19e-29 128
MsaG036264 MtrunA17_Chr5g0394201 24.472 568 324 18 641 1196 385 859 1.05e-23 108
MsaG036264 MtrunA17_Chr4g0052341 25.000 540 317 20 637 1160 763 1230 7.54e-23 106
MsaG036264 MtrunA17_Chr1g0190391 29.289 239 139 9 600 833 576 789 4.12e-22 103
MsaG036264 MtrunA17_Chr1g0205891 25.251 499 283 15 641 1127 398 818 2.36e-21 101
MsaG036264 MtrunA17_Chr3g0137091 24.395 496 303 16 641 1127 311 743 3.41e-21 100
MsaG036264 MtrunA17_Chr4g0040301 26.174 596 292 27 647 1158 18 549 1.10e-20 98.6
MsaG036264 MtrunA17_Chr4g0066741 24.078 515 298 17 641 1146 210 640 4.99e-20 97.1
MsaG036264 MtrunA17_Chr2g0286291 24.466 515 296 18 641 1146 213 643 8.34e-19 92.8
MsaG036264 MtrunA17_Chr1g0190381 34.969 163 94 4 996 1149 196 355 1.69e-18 90.9
MsaG036264 MtrunA17_Chr4g0066651 22.951 488 305 12 647 1127 1017 1440 2.60e-16 85.1
MsaG036264 MtrunA17_Chr3g0114111 64.815 54 19 0 1142 1195 1 54 4.62e-14 68.6
MsaG036264 MtrunA17_Chr7g0257101 31.250 176 113 5 971 1145 1173 1341 1.39e-12 72.8
MsaG036264 MtrunA17_Chr3g0095091 29.609 179 115 4 957 1133 856 1025 1.53e-12 72.8
MsaG036264 MtrunA17_Chr5g0442141 30.168 179 114 4 957 1133 902 1071 2.15e-12 72.0
MsaG036264 MtrunA17_Chr1g0183681 30.714 140 91 3 1005 1144 750 883 4.94e-12 70.9
MsaG036264 MtrunA17_Chr2g0320751 35.000 120 72 3 1008 1127 868 981 2.85e-11 68.2
MsaG036264 MtrunA17_Chr1g0168031 29.932 147 95 4 983 1127 978 1118 8.30e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036264 AT3G42670.2 60.714 784 280 11 422 1187 480 1253 0.0 944
MsaG036264 AT3G42670.2 35.737 319 188 7 4 319 3 307 3.77e-49 191
MsaG036264 AT3G42670.3 60.714 784 280 11 422 1187 480 1253 0.0 944
MsaG036264 AT3G42670.3 35.737 319 188 7 4 319 3 307 3.77e-49 191
MsaG036264 AT3G42670.1 60.714 784 280 11 422 1187 480 1253 0.0 944
MsaG036264 AT3G42670.1 35.737 319 188 7 4 319 3 307 3.77e-49 191
MsaG036264 AT3G42670.4 60.560 786 280 11 422 1187 480 1255 0.0 943
MsaG036264 AT3G42670.4 35.737 319 188 7 4 319 3 307 4.19e-49 191
MsaG036264 AT5G20420.1 60.614 782 283 12 423 1187 486 1259 0.0 938
MsaG036264 AT5G20420.1 36.145 332 171 11 4 317 2 310 3.25e-45 179
MsaG036264 AT3G24340.1 33.062 738 421 16 447 1177 443 1114 1.52e-107 365
MsaG036264 AT1G05490.1 32.102 785 436 25 462 1209 685 1409 3.97e-105 363
MsaG036264 AT2G16390.2 31.701 735 407 23 428 1144 193 850 2.22e-89 309
MsaG036264 AT2G16390.1 31.701 735 407 23 428 1144 190 847 2.25e-89 309
MsaG036264 AT2G21450.3 32.214 655 362 20 508 1144 92 682 7.42e-85 292
MsaG036264 AT2G21450.1 32.214 655 362 20 508 1144 183 773 2.08e-84 293
MsaG036264 AT2G21450.2 32.214 655 362 20 508 1144 190 780 2.24e-84 293
MsaG036264 AT3G19210.2 25.199 627 358 23 606 1197 180 730 3.92e-31 133
MsaG036264 AT3G19210.1 24.483 629 362 22 606 1197 180 732 9.65e-31 131
MsaG036264 AT3G19210.3 24.483 629 362 22 606 1197 180 732 1.70e-30 130
MsaG036264 AT5G63950.1 25.088 570 334 19 596 1136 367 872 2.03e-29 127
MsaG036264 AT5G63950.2 25.265 566 321 20 596 1136 367 855 5.31e-28 123
MsaG036264 AT1G08600.4 28.807 243 131 8 602 833 715 926 6.58e-23 107
MsaG036264 AT1G08600.4 33.735 166 92 4 996 1149 1123 1282 1.34e-17 89.7
MsaG036264 AT1G08600.3 28.807 243 131 8 602 833 715 926 6.58e-23 107
MsaG036264 AT1G08600.3 33.735 166 92 4 996 1149 1123 1282 1.34e-17 89.7
MsaG036264 AT1G08600.2 28.807 243 131 8 602 833 715 926 6.58e-23 107
MsaG036264 AT1G08600.2 33.735 166 92 4 996 1149 1123 1282 1.34e-17 89.7
MsaG036264 AT3G06010.1 25.874 572 312 23 641 1196 425 900 5.38e-22 103
MsaG036264 AT1G03750.1 24.712 607 302 25 639 1158 159 697 2.41e-21 101
MsaG036264 AT4G31900.3 23.626 546 300 19 596 1127 214 656 3.93e-21 100
MsaG036264 AT4G31900.2 23.732 552 292 20 596 1127 173 615 4.14e-21 100
MsaG036264 AT4G31900.1 23.732 552 292 20 596 1127 214 656 4.84e-21 100
MsaG036264 AT2G25170.1 24.803 508 288 20 640 1127 294 727 6.87e-19 94.0
MsaG036264 AT2G25170.4 24.803 508 288 20 640 1127 294 727 6.87e-19 94.0
MsaG036264 AT2G25170.2 24.803 508 288 20 640 1127 311 744 8.25e-19 93.6
MsaG036264 AT2G25170.3 24.803 508 288 20 640 1127 320 753 8.85e-19 93.6
MsaG036264 AT3G06400.2 23.002 513 306 15 641 1146 211 641 3.64e-18 91.3
MsaG036264 AT3G06400.1 23.002 513 306 15 641 1146 211 641 3.79e-18 91.3
MsaG036264 AT3G06400.3 23.002 513 306 15 641 1146 211 641 4.43e-18 90.9
MsaG036264 AT1G08600.1 33.735 166 92 4 996 1149 1102 1261 1.19e-17 89.7
MsaG036264 AT5G66750.1 31.731 208 126 6 1006 1212 540 732 4.38e-17 87.4
MsaG036264 AT2G44980.1 25.714 525 288 22 647 1150 80 523 2.66e-14 78.6
MsaG036264 AT3G57300.1 34.483 145 89 3 1006 1150 1222 1360 1.96e-13 75.9
MsaG036264 AT3G57300.4 34.483 145 89 3 1006 1150 1222 1360 1.96e-13 75.9
MsaG036264 AT3G57300.2 34.483 145 89 3 1006 1150 1255 1393 2.27e-13 75.9
MsaG036264 AT2G44980.3 24.908 546 299 23 640 1150 72 541 3.49e-13 74.7
MsaG036264 AT2G44980.2 25.046 539 293 23 647 1150 80 542 5.62e-13 74.3
MsaG036264 AT2G13370.1 30.978 184 110 6 957 1136 910 1080 8.65e-13 73.9
MsaG036264 AT2G13370.2 30.978 184 110 6 957 1136 910 1080 8.65e-13 73.9
MsaG036264 AT2G13370.3 30.978 184 110 6 957 1136 910 1080 8.65e-13 73.9
MsaG036264 AT5G19310.2 29.843 191 121 4 987 1176 427 605 1.18e-12 73.2
MsaG036264 AT5G19310.1 29.843 191 121 4 987 1176 691 869 1.44e-12 73.2
MsaG036264 AT5G43530.1 31.410 156 97 5 975 1127 1094 1242 2.68e-12 72.0
MsaG036264 AT2G28290.3 32.298 161 101 4 1015 1173 1094 1248 3.61e-12 72.0
MsaG036264 AT2G28290.4 32.298 161 101 4 1015 1173 1094 1248 3.61e-12 72.0
MsaG036264 AT2G28290.6 32.298 161 101 4 1015 1173 1095 1249 3.64e-12 72.0
MsaG036264 AT2G28290.2 32.298 161 101 4 1015 1173 1094 1248 3.70e-12 72.0
MsaG036264 AT2G28290.1 32.298 161 101 4 1015 1173 1094 1248 3.86e-12 72.0
MsaG036264 AT2G28290.5 32.298 161 101 4 1015 1173 1095 1249 3.90e-12 72.0
MsaG036264 AT5G05130.1 34.959 123 74 3 1006 1127 725 842 5.01e-12 71.2
MsaG036264 AT2G18760.3 37.500 96 59 1 1049 1144 840 934 1.16e-11 70.1
MsaG036264 AT2G18760.4 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1
MsaG036264 AT2G18760.1 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1
MsaG036264 AT2G18760.2 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1
MsaG036264 AT2G18760.7 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1
MsaG036264 AT2G18760.5 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1
MsaG036264 AT2G18760.6 37.500 96 59 1 1049 1144 781 875 1.20e-11 70.1

Find 194 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACACTTACATGAAGAATT+TGG 0.169887 7:-30589824 MsaT036264.1:CDS
GCTCAGAACAAATCTTATTT+TGG 0.176054 7:+30587792 None:intergenic
AGCACTTCGTGAACAAATTT+TGG 0.230747 7:+30588276 None:intergenic
AGGGCTTTCTCTCAAAGTTT+TGG 0.255357 7:+30588458 None:intergenic
GAATGAAACCTTCTTTAAAA+AGG 0.258801 7:+30590503 None:intergenic
CTGAAGTGGTTAAGCATTAT+AGG 0.265442 7:-30590617 MsaT036264.1:CDS
AACCTTGATTTAGTGCTTCT+AGG 0.271299 7:+30588405 None:intergenic
TTTGTTTCTTCATTTATAAT+TGG 0.272218 7:+30589778 None:intergenic
TATACGTTCAATGAAGAAAT+TGG 0.272322 7:-30589430 MsaT036264.1:CDS
CCTGGAGTTCCAAGACCTTC+TGG 0.279541 7:-30588618 MsaT036264.1:CDS
AAACAGCATTTGAAGCAATA+AGG 0.285770 7:-30591651 MsaT036264.1:intron
AATTCATGGTTCGGATGATA+TGG 0.288963 7:-30589980 MsaT036264.1:CDS
CCATGAATTCGCCTCCTTGC+AGG 0.300379 7:+30589994 None:intergenic
AAGATTAAACTTACTGGAAT+AGG 0.303498 7:+30591427 None:intergenic
GTCCAACCCGATTTGACATT+TGG 0.313761 7:+30591449 None:intergenic
TGATGACTTGTCTGCCATTT+GGG 0.322544 7:-30589707 MsaT036264.1:CDS
TTCCAATGGCACCTAGAAAA+AGG 0.326300 7:-30594193 None:intergenic
CCAGGAAGAACCGTAGGTTT+TGG 0.328085 7:+30588636 None:intergenic
TGGTGATGTAAAACATGTTT+TGG 0.329183 7:-30588598 MsaT036264.1:CDS
CAGCGGTTCAAAGATACTTC+TGG 0.337244 7:-30587581 MsaT036264.1:CDS
ACAGAAACTGCAGAAGAAAA+TGG 0.339883 7:-30587977 MsaT036264.1:CDS
AAATACTTTCAATGGCAAAA+TGG 0.340921 7:-30587682 MsaT036264.1:CDS
CTGATGACTTGTCTGCCATT+TGG 0.349617 7:-30589708 MsaT036264.1:CDS
CTCTACCTCGAGAGGGTAAC+TGG 0.351257 7:+30587942 None:intergenic
CCAAAACCTACGGTTCTTCC+TGG 0.354433 7:-30588636 MsaT036264.1:CDS
CAGCTTCGTGAGGTTGTCTC+TGG 0.354705 7:+30590811 None:intergenic
ATCAAATTTGATTTAAGAAA+AGG 0.357435 7:-30587817 MsaT036264.1:CDS
TTGTGGAAGATCCTTCTAAA+TGG 0.362063 7:-30587321 MsaT036264.1:CDS
AAAGAGGTTCTGCTACTTAC+TGG 0.365932 7:-30587658 MsaT036264.1:CDS
TTCATCAACGTTTATGAAAG+TGG 0.366193 7:-30588078 MsaT036264.1:CDS
GTTGATGATAATGTTACTTT+GGG 0.371974 7:-30590045 MsaT036264.1:CDS
TGTTACAATATCAAGTGATT+TGG 0.373273 7:-30590070 MsaT036264.1:CDS
CCTTCTAGAAGGATCAGATT+TGG 0.373446 7:-30587164 MsaT036264.1:CDS
TTGAGTATCCATAAAATGTA+AGG 0.379955 7:+30591576 None:intergenic
AGAAGAACCTATGTAACGTT+TGG 0.381332 7:+30590245 None:intergenic
AATTCCTCTGATACACTTCC+TGG 0.382519 7:-30588054 MsaT036264.1:CDS
AAACGAAATGGGAAGGGATT+CGG 0.382759 7:-30589951 MsaT036264.1:CDS
CTATGCTCCAAATGTCAAAT+CGG 0.384080 7:-30591456 MsaT036264.1:CDS
GCAGCAAGTTGTCTTCTTGA+TGG 0.386624 7:-30589664 MsaT036264.1:CDS
TCATCAAGTATCAAGATTCC+AGG 0.388586 7:+30588438 None:intergenic
AAGGTTGAGGAAATGCTTAA+TGG 0.392142 7:-30588388 MsaT036264.1:CDS
GTCTGCCATTTGGGAAGAAA+TGG 0.392168 7:-30589698 MsaT036264.1:CDS
GAAGAATTTGGATAGTCTTC+CGG 0.394022 7:-30589812 MsaT036264.1:CDS
AGTTACTTGAAGTTATTTCC+TGG 0.397468 7:-30588786 MsaT036264.1:CDS
TTGACAAACAGAAGTAATAA+AGG 0.397493 7:-30589886 MsaT036264.1:CDS
GCAGCATTTGCTTGAGGCGC+GGG 0.398158 7:-30588202 MsaT036264.1:CDS
CGAACGAGGCCATACAAGAT+AGG 0.400717 7:-30590195 MsaT036264.1:CDS
GTCATGTTCACAAATTTCAT+TGG 0.407210 7:+30589453 None:intergenic
CCTGCAAGGAGGCGAATTCA+TGG 0.412723 7:-30589994 MsaT036264.1:CDS
TATTTGAGAAATACTTTCAA+TGG 0.412834 7:-30587690 MsaT036264.1:CDS
ATCACAGTCAAGATTAGTCT+TGG 0.426980 7:-30590565 MsaT036264.1:CDS
TCACAGTCAAGATTAGTCTT+GGG 0.431530 7:-30590564 MsaT036264.1:CDS
GGATGGTAAGAGTGGTGAAA+AGG 0.434748 7:-30590157 MsaT036264.1:CDS
GATCTAGATTTGTTTGAAAG+AGG 0.437409 7:-30587634 MsaT036264.1:CDS
GAAGAACCTATGTAACGTTT+GGG 0.437927 7:+30590246 None:intergenic
AGTTGATGATAATGTTACTT+TGG 0.438485 7:-30590046 MsaT036264.1:CDS
TTCACAACATTCATAAAAGA+AGG 0.444285 7:+30590414 None:intergenic
TGTGAACTTCAAATCGGAAA+AGG 0.444435 7:-30590397 MsaT036264.1:CDS
GTTACTTGAAGTTATTTCCT+GGG 0.446436 7:-30588785 MsaT036264.1:CDS
CTCTCTGTGTTCGACGGCCA+TGG 0.448149 7:+30588670 None:intergenic
AAATAAGCTCCTATCTTGTA+TGG 0.448197 7:+30590186 None:intergenic
CATGTGTAAAGTGTGGATTT+TGG 0.450682 7:+30588741 None:intergenic
AGGTTCTTCTGTTGAGAAGT+TGG 0.452731 7:-30590232 MsaT036264.1:CDS
CCAAATCTGATCCTTCTAGA+AGG 0.453881 7:+30587164 None:intergenic
TGTTTGTCAAACAAATTGAA+TGG 0.453921 7:+30589899 None:intergenic
GTAAATTTCTACGAGGATCA+AGG 0.454603 7:-30590765 MsaT036264.1:CDS
TTGCATGAGGGATGGTTCCA+TGG 0.456415 7:+30588893 None:intergenic
GAATGTTGTGAACTTCAAAT+CGG 0.456729 7:-30590403 MsaT036264.1:CDS
TAGCCTCTTCAATTCATCTT+CGG 0.456753 7:+30587846 None:intergenic
GGACAGCAAGGCCTTCTAGA+AGG 0.458099 7:-30587175 MsaT036264.1:CDS
ATATCACATGCTCCTGGAGC+TGG 0.460717 7:-30588840 MsaT036264.1:CDS
GATAGGAGCTTATTTGATAG+AGG 0.460980 7:-30590178 MsaT036264.1:CDS
TGCAAGAGCCACAGTCTGTT+AGG 0.460999 7:-30587138 MsaT036264.1:CDS
TGCGAATATCAGATGACAAA+GGG 0.468200 7:+30591542 None:intergenic
TATGATTATGAAGAATGAAA+AGG 0.468297 7:-30587230 MsaT036264.1:CDS
CGCAGCATTTGCTTGAGGCG+CGG 0.471984 7:-30588203 MsaT036264.1:CDS
ATGTCATTAAATCAAATTGA+AGG 0.472208 7:-30590300 MsaT036264.1:CDS
GTGTATCAGTTATTGACGAC+CGG 0.474170 7:-30587418 MsaT036264.1:CDS
TATAAATGAAGAAACAAAGT+TGG 0.475973 7:-30589773 MsaT036264.1:CDS
TCAATTTGATTTAATGACAT+TGG 0.476376 7:+30590303 None:intergenic
AGATTGAAGATGAAATACTT+AGG 0.478427 7:-30587288 MsaT036264.1:CDS
TTTCCGATCTTTCTTGCATG+AGG 0.479980 7:+30588880 None:intergenic
AAGAAGAATGGTGCTGCTGC+TGG 0.481843 7:-30590093 MsaT036264.1:CDS
AATAAAGAGGTTTATGCGAC+TGG 0.484157 7:-30590714 MsaT036264.1:CDS
TGGAACTTCCTACAGAATTG+AGG 0.485813 7:-30588368 MsaT036264.1:CDS
AGTATCCATTTCTTCCCAAA+TGG 0.486016 7:+30589693 None:intergenic
AAATATCTTTCAAAGAAGAA+TGG 0.486991 7:-30590105 MsaT036264.1:CDS
ATTTGATTTAATGACATTGG+CGG 0.487064 7:+30590306 None:intergenic
TCAATGGCAAAATGGAAAAG+AGG 0.487364 7:-30587674 MsaT036264.1:CDS
GATTGAAGATGAAATACTTA+GGG 0.488087 7:-30587287 MsaT036264.1:CDS
ATTTGCTTGAGGCGCGGGCG+AGG 0.490931 7:-30588197 MsaT036264.1:CDS
TTTACATCACCAGAAGGTCT+TGG 0.492630 7:+30588609 None:intergenic
ATAAGTATCGAAGAACTACT+TGG 0.498443 7:-30587381 MsaT036264.1:CDS
CAAATGGCAGACAAGTCATC+AGG 0.504596 7:+30589709 None:intergenic
TATGCTCCAAATGTCAAATC+GGG 0.504825 7:-30591455 MsaT036264.1:CDS
AACCTATGTAACGTTTGGGT+TGG 0.506431 7:+30590250 None:intergenic
TCAATCAACGCTTGTGCTGA+AGG 0.506468 7:-30587556 MsaT036264.1:CDS
GTGAACTTCAAATCGGAAAA+GGG 0.506922 7:-30590396 MsaT036264.1:CDS
ACACCCAAGTGTTCTTGTCA+TGG 0.509409 7:-30588544 MsaT036264.1:CDS
AAGAAACGAAATGACGAGAA+AGG 0.509910 7:+30588812 None:intergenic
TCTGTTGAGAAGTTGGAAGT+TGG 0.511537 7:-30590225 MsaT036264.1:CDS
CACCCAAGTGTTCTTGTCAT+GGG 0.512599 7:-30588543 MsaT036264.1:CDS
TTACGATAAGCATCATCAAA+TGG 0.513234 7:+30594152 None:intergenic
ACCATGGATGTATCGATCCA+AGG 0.517448 7:+30587908 None:intergenic
ATCAAAAGCTCTACCTCGAG+AGG 0.518482 7:+30587934 None:intergenic
GTGCCTGTGTATCTTATCCA+TGG 0.520986 7:-30588687 MsaT036264.1:CDS
GGAGCTTATTTGATAGAGGA+TGG 0.523316 7:-30590174 MsaT036264.1:CDS
GTTATTGACGACCGGGTCGA+TGG 0.524989 7:-30587410 MsaT036264.1:CDS
ATAATTGGCTCTTCTTCAGC+CGG 0.534892 7:+30589793 None:intergenic
AGATGAAATACTTAGGGAGA+TGG 0.537029 7:-30587281 MsaT036264.1:CDS
CCAGAAGGTCTTGGAACTCC+AGG 0.539229 7:+30588618 None:intergenic
GATCTTTCTTGCATGAGGGA+TGG 0.540113 7:+30588885 None:intergenic
AGTAAAATCCTCAATTCTGT+AGG 0.540703 7:+30588360 None:intergenic
AAGGAGGCGAATTCATGGTT+CGG 0.541286 7:-30589989 MsaT036264.1:CDS
CACAGTCAAGATTAGTCTTG+GGG 0.541514 7:-30590563 MsaT036264.1:CDS
ATCCTAGAAGCACTAAATCA+AGG 0.543534 7:-30588407 MsaT036264.1:CDS
TATAATCTCAAGCTGTTCAT+CGG 0.543979 7:+30587999 None:intergenic
TTGATAGAGGATGGTAAGAG+TGG 0.544017 7:-30590165 MsaT036264.1:CDS
GAAAGCGCAGCATTTGCTTG+AGG 0.546756 7:-30588208 MsaT036264.1:CDS
TTCCGATCTTTCTTGCATGA+GGG 0.548848 7:+30588881 None:intergenic
ATCCCTCATGCAAGAAAGAT+CGG 0.551602 7:-30588883 MsaT036264.1:CDS
TGTGTCATATCACATGCTCC+TGG 0.551693 7:-30588846 MsaT036264.1:CDS
GGAATCTTGATACTTGATGA+AGG 0.552776 7:-30588435 MsaT036264.1:CDS
TCTGCAAAGAGAAGCCTGCA+AGG 0.554610 7:-30590008 MsaT036264.1:CDS
GGAAGAAATGGATACTGCTA+TGG 0.554695 7:-30589686 MsaT036264.1:CDS
AAATGAAGAAACAAAGTTGG+AGG 0.554893 7:-30589770 MsaT036264.1:CDS
ATCACGAGGCATATTCGTTA+CGG 0.555592 7:+30587204 None:intergenic
CTTCAAAATAATAGTTGCGA+AGG 0.555833 7:-30590663 MsaT036264.1:CDS
CCATGGATGTATCGATCCAA+GGG 0.557758 7:+30587909 None:intergenic
CTCCAAATGTCAAATCGGGT+TGG 0.559335 7:-30591451 MsaT036264.1:CDS
AGTTAGATTCTAAATACTTG+AGG 0.561826 7:-30588248 MsaT036264.1:CDS
AAATAGTGATAGTTCTGAAG+TGG 0.562045 7:-30590631 MsaT036264.1:CDS
GTTAAATGTTGATGCACAGA+AGG 0.562995 7:-30589854 MsaT036264.1:CDS
TAAGATTACGTATCATGTTG+TGG 0.564409 7:-30590460 MsaT036264.1:CDS
AAGAACTACTTGGAAAGAGT+GGG 0.567137 7:-30587371 MsaT036264.1:CDS
TAGCCCATGACAAGAACACT+TGG 0.567881 7:+30588540 None:intergenic
TGGATAAGATACACAGGCAC+GGG 0.569069 7:+30588690 None:intergenic
GGTAAGAGTGGTGAAAAGGT+AGG 0.572115 7:-30590153 MsaT036264.1:CDS
TTGCCGAAGATGAATTGAAG+AGG 0.577257 7:-30587849 MsaT036264.1:CDS
CATCAAGTATCAAGATTCCA+GGG 0.577717 7:+30588439 None:intergenic
GAAGAACTACTTGGAAAGAG+TGG 0.579673 7:-30587372 MsaT036264.1:CDS
CCCTTGGATCGATACATCCA+TGG 0.580673 7:-30587909 MsaT036264.1:CDS
TACGAGGATCAAGGTTCCCT+AGG 0.580792 7:-30590756 MsaT036264.1:CDS
ACTTAGGGAGATGGTTGAAG+AGG 0.582988 7:-30587272 MsaT036264.1:CDS
GTCCAACCCAAACGTTACAT+AGG 0.585169 7:-30590252 MsaT036264.1:CDS
TTGTCTTCTTGATGGAACTG+AGG 0.585263 7:-30589656 MsaT036264.1:intron
GTTGCAGGGTTCAAATGTAG+AGG 0.586141 7:-30589488 MsaT036264.1:intron
AGTGTCGAAAAGCGATGATG+AGG 0.586488 7:-30589740 MsaT036264.1:CDS
GAAGCACTAAATCAAGGTTG+AGG 0.587727 7:-30588401 MsaT036264.1:CDS
GGAATATTCTGTACTAGCTG+TGG 0.590871 7:-30589409 MsaT036264.1:CDS
ATTGACGACCGGGTCGATGG+AGG 0.593637 7:-30587407 MsaT036264.1:CDS
GATGAATTGAAGAGGCTAGA+CGG 0.597436 7:-30587841 MsaT036264.1:CDS
TCACAACATTCATAAAAGAA+GGG 0.597727 7:+30590415 None:intergenic
GAAAAGGTAGGCGATGCTGC+TGG 0.601604 7:-30590141 MsaT036264.1:CDS
AGTGTATATATTTGTAAACC+AGG 0.603594 7:+30588036 None:intergenic
AGTGGTTAAGCATTATAGGT+GGG 0.609331 7:-30590613 MsaT036264.1:CDS
CTTATCTTCCTCCATCGACC+CGG 0.610407 7:+30587399 None:intergenic
AAGTGGTTAAGCATTATAGG+TGG 0.612238 7:-30590614 MsaT036264.1:CDS
ATTTGCACACAGAAAGTACA+TGG 0.612438 7:-30588481 MsaT036264.1:CDS
AAGCAATAAGGAATGATGCA+TGG 0.613601 7:-30591639 MsaT036264.1:CDS
GGAACTCCAGGAAGAACCGT+AGG 0.613738 7:+30588630 None:intergenic
AAAACTTTGAGAGAAAGCCC+TGG 0.619123 7:-30588456 MsaT036264.1:CDS
TTGAATCTCTCTGTGTTCGA+CGG 0.624793 7:+30588664 None:intergenic
ATGCGAATATCAGATGACAA+AGG 0.625364 7:+30591541 None:intergenic
CAAAATCCACACTTTACACA+TGG 0.625539 7:-30588740 MsaT036264.1:CDS
GATTCTAAATACTTGAGGAG+AGG 0.628538 7:-30588243 MsaT036264.1:CDS
TAAACCAGGAAGTGTATCAG+AGG 0.630562 7:+30588050 None:intergenic
TGTATCAGTTATTGACGACC+GGG 0.631867 7:-30587417 MsaT036264.1:CDS
TCGAACACAGAGAGATTCAA+CGG 0.634741 7:-30588661 MsaT036264.1:CDS
TGCTGTCCTTCTGTATCACG+AGG 0.637077 7:+30587190 None:intergenic
CAAGAAAGATCGGAAACAAG+TGG 0.645212 7:-30588873 MsaT036264.1:CDS
TTCAACGGCTCCAAAACCTA+CGG 0.645818 7:-30588646 MsaT036264.1:CDS
AAAGAGGAGAAACAATACAG+TGG 0.648975 7:-30589119 MsaT036264.1:CDS
ATGGATAAGATACACAGGCA+CGG 0.650745 7:+30588689 None:intergenic
ACAAACAGAAGTAATAAAGG+TGG 0.652555 7:-30589883 MsaT036264.1:CDS
TCAAAAGCTCTACCTCGAGA+GGG 0.652702 7:+30587935 None:intergenic
GAGCATTGATCAGCTTCGTG+AGG 0.658839 7:+30590801 None:intergenic
CGGCCATGGATAAGATACAC+AGG 0.663444 7:+30588684 None:intergenic
AAAGGGATCAATAGTACCTG+TGG 0.665894 7:-30590379 MsaT036264.1:CDS
GAATGATGCATGGCAACCTG+TGG 0.667323 7:-30591629 MsaT036264.1:CDS
CGTGATACAGAAGGACAGCA+AGG 0.667482 7:-30587187 MsaT036264.1:CDS
TACCACCAGTTACCCTCTCG+AGG 0.667880 7:-30587947 MsaT036264.1:CDS
GAAAGATCGGAAACAAGTGG+TGG 0.673137 7:-30588870 MsaT036264.1:CDS
GCGAATATCAGATGACAAAG+GGG 0.675109 7:+30591543 None:intergenic
ATATCGAATAGAGAAACCAC+AGG 0.679700 7:+30590363 None:intergenic
TTTGCACCATGTGTAAAGTG+TGG 0.681578 7:+30588734 None:intergenic
AGCCCATGACAAGAACACTT+GGG 0.690203 7:+30588541 None:intergenic
AGTATCTTTGAACCGCTGCG+AGG 0.704953 7:+30587586 None:intergenic
AATCAACTCAGATAACGATG+AGG 0.714220 7:-30588145 MsaT036264.1:CDS
AATATGCCTCGTGATACAGA+AGG 0.715485 7:-30587196 MsaT036264.1:CDS
AAGTTTGAAGATCCTCGCAG+CGG 0.715513 7:-30587598 MsaT036264.1:CDS
GCCTAAGACGATCAAAACGT+AGG 0.727623 7:-30590278 MsaT036264.1:CDS
GCAAAGAGAAGCCTGCAAGG+AGG 0.740126 7:-30590005 MsaT036264.1:CDS
TACCTCGAGAGGGTAACTGG+TGG 0.749946 7:+30587945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 30587132 30594210 30587132 ID=MsaG036264
Chr7 mRNA 30587132 30594210 30587132 ID=MsaT036264.1;Parent=MsaG036264
Chr7 exon 30587132 30589162 30587132 ID=MsaT036264.1.exon5;Parent=MsaT036264.1
Chr7 CDS 30587132 30589162 30587132 ID=cds.MsaT036264.1;Parent=MsaT036264.1
Chr7 exon 30589369 30589503 30589369 ID=MsaT036264.1.exon4;Parent=MsaT036264.1
Chr7 CDS 30589369 30589503 30589369 ID=cds.MsaT036264.1;Parent=MsaT036264.1
Chr7 exon 30589657 30590862 30589657 ID=MsaT036264.1.exon3;Parent=MsaT036264.1
Chr7 CDS 30589657 30590862 30589657 ID=cds.MsaT036264.1;Parent=MsaT036264.1
Chr7 exon 30591441 30591667 30591441 ID=MsaT036264.1.exon2;Parent=MsaT036264.1
Chr7 CDS 30591441 30591667 30591441 ID=cds.MsaT036264.1;Parent=MsaT036264.1
Chr7 exon 30594156 30594210 30594156 ID=MsaT036264.1.exon1;Parent=MsaT036264.1
Chr7 CDS 30594156 30594210 30594156 ID=cds.MsaT036264.1;Parent=MsaT036264.1
Gene Sequence

>MsaT036264.1

ATGGCACCTAGAAAAAGGCATTTGCAACAATTGAATCCATTTGATGATGCTTATCCATTTGAAGCAATAAGGAATGATGCATGGCAACCTGTGGAGCTTATAAAAATTGAGTTTGGAAAAACAACCTTACATTTTATGGATACTCAACAAATGCAAACCCCTTTGTCATCTGATATTCGCATAAGATCAAGAAAAGCTACTTCATTTGATTGCTCTAACTTTTTACGACGCGGAATTGACATATCTATGCTCCAAATGTCAAATCGGGTTGGACCTATTCCAACGTCAATCGATGCAAGAATAAATTCAATCCAGAGACAACCTCACGAAGCTGATCAATGCTCATGTCAGTTTTATGTAAATTTCTACGAGGATCAAGGTTCCCTAGGTAGAAATATAAAAACACTTAATAAAGAGGTTTATGCGACTGGAATAGATCGAATTTTCATCCTCCAACGGCTTCAAAATAATAGTTGCGAAGGCTTAATAAAAAATAGTGATAGTTCTGAAGTGGTTAAGCATTATAGGTGGGATTCATCTGAAGATTGCTCTTCATTATCACAGTCAAGATTAGTCTTGGGGAGATTTTTATCTGATCTTTCGTGGTTCGTTACAACATCCTTTTTAAAGAAGGTTTCATTCAGTATCCGATCTGTTCAAAATAAGATTACGTATCATGTTGTGGAAAACAATACTGATATTATTGACCCTTCTTTTATGAATGTTGTGAACTTCAAATCGGAAAAGGGATCAATAGTACCTGTGGTTTCTCTATTCGATATGTTTTGTGATTATGATGAAGAAGAAGATGAAGAGCCGCCAATGTCATTAAATCAAATTGAAGGCCTAAGACGATCAAAACGTAGGAATGTCCAACCCAAACGTTACATAGGTTCTTCTGTTGAGAAGTTGGAAGTTGGTACTTTTCGAACGAGGCCATACAAGATAGGAGCTTATTTGATAGAGGATGGTAAGAGTGGTGAAAAGGTAGGCGATGCTGCTGGCGAAATTCCTTCAAAATATCTTTCAAAGAAGAATGGTGCTGCTGCTGGTGTTACAATATCAAGTGATTTGGAAGTTGATGATAATGTTACTTTGGGACATTATTATAGCTTCTGCAAAGAGAAGCCTGCAAGGAGGCGAATTCATGGTTCGGATGATATGGATTTTGAAACGAAATGGGAAGGGATTCGGTTCAAAAAAGGAGCTCAAACAAAAAGATACCATTCAATTTGTTTGACAAACAGAAGTAATAAAGGTGGCAGAACGTTAAATGTTGATGCACAGAAGGAAATAATTGACACTTACATGAAGAATTTGGATAGTCTTCCGGCTGAAGAAGAGCCAATTATAAATGAAGAAACAAAGTTGGAGGAAGAAAAAGTGTCGAAAAGCGATGATGAGGAAGAAAATCCTGATGACTTGTCTGCCATTTGGGAAGAAATGGATACTGCTATGGCAGCAAGTTGTCTTCTTGATGGAACTGAGGGTTCAAATGTAGAGGTTTTAGCAGACACCAATGAAATTTGTGAACATGACTATACGTTCAATGAAGAAATTGGAATATTCTGTACTAGCTGTGGCTCTGTGAAAACTGAGATACGAGACATTACTGAACTCGTTGTGGAAAGACAAAAATGGCAGAAAGAGGAGAAACAATACAGTGGAGAAGTTAATGATGAACAAAAATCAGAGGCAAAAGTTGATGAAGATGATAATAATGATATGTTCTCGACTCATGCTACTGATCGTGATGAACCAATACCTGCAGAAAAAGACAATGTTTGGGAATTGATTCCAGAACTCAAAGAAAAGATGCATGCGCACCAGCAAAAAGCTTTTGAGTTTCTTTGGAAAAACATTGCTGGCTCCATGGAACCATCCCTCATGCAAGAAAGATCGGAAACAAGTGGTGGATGTGTCATATCACATGCTCCTGGAGCTGGAAAAACCTTTCTCGTCATTTCGTTTCTTGTAAGTTACTTGAAGTTATTTCCTGGGAAAAGACCATTAGTCCTTGCACCAAAATCCACACTTTACACATGGTGCAAAGAGTTCAAAAAATGGAAAGTTCCCGTGCCTGTGTATCTTATCCATGGCCGTCGAACACAGAGAGATTCAACGGCTCCAAAACCTACGGTTCTTCCTGGAGTTCCAAGACCTTCTGGTGATGTAAAACATGTTTTGGATTGTCTTGGAAAAATAAAAAAGTGGCATTCACACCCAAGTGTTCTTGTCATGGGCTATACTTCATTTTTAGCCTTAATGAGACAAGATACAAAATTTGCACACAGAAAGTACATGGCCAAAACTTTGAGAGAAAGCCCTGGAATCTTGATACTTGATGAAGGTCACAATCCTAGAAGCACTAAATCAAGGTTGAGGAAATGCTTAATGGAACTTCCTACAGAATTGAGGATTTTACTTTCTGGCACTTTGTTTCAGAACAATTTCTGCGAATATTTCAACACATTGTGTTTAGCAAGACCAAAATTTGTTCACGAAGTGCTACGAGAGTTAGATTCTAAATACTTGAGGAGAGGTAATAGAGAGAAGAAAGCGCAGCATTTGCTTGAGGCGCGGGCGAGGAAATTCTTCTTGAACAACATTGCTACGAAAATCAACTCAGATAACGATGAGGAAAAAATGCAGGGTCTACATGTGCTGCGAAAGATCACGAGCAGTTTCATCAACGTTTATGAAAGTGGAAATTCCTCTGATACACTTCCTGGTTTACAAATATATACACTGTTCATGAATACGTCCGATGAACAGCTTGAGATTATACAGAAACTGCAGAAGAAAATGGCTGAGTGTACCACCAGTTACCCTCTCGAGGTAGAGCTTTTGATAACCCTTGGATCGATACATCCATGGTTGATCAAAACAGCAACACCATGTGCAGATAAGTTTTTTGCCGAAGATGAATTGAAGAGGCTAGACGGAATCAAATTTGATTTAAGAAAAGGTTCCAAAATAAGATTTGTTCTGAGCCTAATCTCACGTGTAGTGAAAAATGAAAAGGTTCTTATCTTTTGTCACTACCTTGCACCTCTGAGATTTTTCATAGAGTTATTTGAGAAATACTTTCAATGGCAAAATGGAAAAGAGGTTCTGCTACTTACTGGTGATCTAGATTTGTTTGAAAGAGGAAAAATGATTGATAAGTTTGAAGATCCTCGCAGCGGTTCAAAGATACTTCTGGCTTCAATCAACGCTTGTGCTGAAGGTATTAGTTTAACAGCTGCTTCTAGAGTGATATTTTTGGATTCTGAATGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGTCCTGGTCAGCAAAAAATGGTTTATGTGTATCAGTTATTGACGACCGGGTCGATGGAGGAAGATAAGTATCGAAGAACTACTTGGAAAGAGTGGGTTTCTTGCATGATTTTTAGTGAGGAGTTTGTGGAAGATCCTTCTAAATGGCAAGCAGAAAAGATTGAAGATGAAATACTTAGGGAGATGGTTGAAGAGGATAAGTCTAAAGCTATTCATATGATTATGAAGAATGAAAAGGATTCCGTAACGAATATGCCTCGTGATACAGAAGGACAGCAAGGCCTTCTAGAAGGATCAGATTTGGTTATGCAAGAGCCACAGTCTGTTAGGCACTAG
Protein sequence

>MsaT036264.1

MAPRKRHLQQLNPFDDAYPFEAIRNDAWQPVELIKIEFGKTTLHFMDTQQMQTPLSSDIRIRSRKATSFDCSNFLRRGIDISMLQMSNRVGPIPTSIDARINSIQRQPHEADQCSCQFYVNFYEDQGSLGRNIKTLNKEVYATGIDRIFILQRLQNNSCEGLIKNSDSSEVVKHYRWDSSEDCSSLSQSRLVLGRFLSDLSWFVTTSFLKKVSFSIRSVQNKITYHVVENNTDIIDPSFMNVVNFKSEKGSIVPVVSLFDMFCDYDEEEDEEPPMSLNQIEGLRRSKRRNVQPKRYIGSSVEKLEVGTFRTRPYKIGAYLIEDGKSGEKVGDAAGEIPSKYLSKKNGAAAGVTISSDLEVDDNVTLGHYYSFCKEKPARRRIHGSDDMDFETKWEGIRFKKGAQTKRYHSICLTNRSNKGGRTLNVDAQKEIIDTYMKNLDSLPAEEEPIINEETKLEEEKVSKSDDEEENPDDLSAIWEEMDTAMAASCLLDGTEGSNVEVLADTNEICEHDYTFNEEIGIFCTSCGSVKTEIRDITELVVERQKWQKEEKQYSGEVNDEQKSEAKVDEDDNNDMFSTHATDRDEPIPAEKDNVWELIPELKEKMHAHQQKAFEFLWKNIAGSMEPSLMQERSETSGGCVISHAPGAGKTFLVISFLVSYLKLFPGKRPLVLAPKSTLYTWCKEFKKWKVPVPVYLIHGRRTQRDSTAPKPTVLPGVPRPSGDVKHVLDCLGKIKKWHSHPSVLVMGYTSFLALMRQDTKFAHRKYMAKTLRESPGILILDEGHNPRSTKSRLRKCLMELPTELRILLSGTLFQNNFCEYFNTLCLARPKFVHEVLRELDSKYLRRGNREKKAQHLLEARARKFFLNNIATKINSDNDEEKMQGLHVLRKITSSFINVYESGNSSDTLPGLQIYTLFMNTSDEQLEIIQKLQKKMAECTTSYPLEVELLITLGSIHPWLIKTATPCADKFFAEDELKRLDGIKFDLRKGSKIRFVLSLISRVVKNEKVLIFCHYLAPLRFFIELFEKYFQWQNGKEVLLLTGDLDLFERGKMIDKFEDPRSGSKILLASINACAEGISLTAASRVIFLDSEWNPSKTKQAIARAFRPGQQKMVYVYQLLTTGSMEEDKYRRTTWKEWVSCMIFSEEFVEDPSKWQAEKIEDEILREMVEEDKSKAIHMIMKNEKDSVTNMPRDTEGQQGLLEGSDLVMQEPQSVRH*